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  • 1
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25112 | 18721 | 2018-08-23 05:48:23 | 25112 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Menmiopsis leidyi was entered into Caspian Sea from Black Sea and mass population constitutes in the southern Caspian Sea. In this surveys, the relationship between length and wet weight measured and length and weight followed by this equation: n=384, R^2=0.87 w=0.003L^9005 . The relationship of dry and wet weight calculated, in this surveys, the ratio of dry weight to wet weight was equal to 1.615% in 12 salinity. In vitro, the clearance rate at 12 and 27 C were 52.48 and 107.33 ml/h, respectively, daily ration at above temperatures were 1.98 and 16.65 respectively. Mnemiopsis leidyi can feed Acartia in accordance to 16.5 mg/1gbw (body weight) at 27 C in one day. The average digestive time in 12 C and 27 C were 77.50 min and 36 min respectively. The digestive value in 27 C was 2.5 fold than 12 C. The result indicated that clearance rate, daily ration and digest were increased in high temperature. Stomach content of Mnemiopsis leidyi was shown that this organism utilized from Acartia and Balanus, in addition, stomach content of kilka including two species of zooplankton from the southern Caspian Sea. Stomach contents of clupeonella cultiventris contain more than 54 percent of Acartia and its nauplii. This frequency observed in cluponella engrauliformis and clupeonella grimmi. On basis of zooplankton sampling in the sea, over 90 percentage of zooplankton populations contain Acartia.
    Keywords: Biology ; Iran ; Caspian Sea ; Menmiopsis leidyi ; Zooplankton ; Population ; Survey ; Feeding ; Cetenophora
    Repository Name: AquaDocs
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25327 | 18721 | 2018-09-12 09:54:28 | 25327 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Present project was conducted in shrimp farm located in east of Chabahar in Sistan and Balouchestan province.Twentieth sampling stations at three locations (i.e. 2 stations in supply channel,13 stations in drainage channel and 5 stations in Gwatar Gulf) were selected to determine physical and chemical factors such as; temperature, salinity, dissolved oxygen, pH, nitrate, nitrite, ammonia, phosphate and biological parameters for example Chlorophyll a, phytoplankton and zooplankton. Sampling was conducted once a month from March 2003 until January 2004. The results revealed different bioenvironmental area in the three locations (i.e. supply channel, drainage channel and Gwatar Gulf) over the study period. Concentration of salinity ,pH , nitatr ,nitrite ,ammonia and chlorophyll a in drainage channel were more than supply channel and Gawtar Gulf. While concentation dissolved oxygen and phosphate were in drainage channel less than Gawtar Gulf.Also, sediment with small grain size (clay and silt) caused uptake phosphate and amount phosphate decreased in drainage channel. Considering with lack of environment standards in Iran shrimp effluent, data compared with others countries standard. Results showed that with considerable farm area, value of temperature, salinity, dissolved oxygen and pH were in permit range in Gwatar Gulf while concentation nitrate, phosphate and chlorophyll a were sometimes more than permit range that could cause plankton bloom. Also, Gwatar Gulf were influence monsoon. Nitate, phosphate, chlorophyll a and phytoplankton increased in gwatar Gulf.Regards with that postmonsoon was simultaneus with shrimp harvest.Therefore, increase some parameters could be caused from shrimp effluent or postmonsoon. Thirty four phytoplankton genus were identified including diatoma, dinoflagellate, cyanophyta and chlorophyta. In each area maxiumum percentage of phytoplankton were belonged to diatoma (63%) in supply channel, cyanophyta (51.7%) in drainage channel and dinoflagellate (94.5%) in Gwatar Gulf.Amount and range chemical and physical factors and plankton composition were different in drainage channel, supply channel and Gwatar Gulf. Cyanophyta could grow more in drainage channel.Percentage of aboundance and diversity of phytoplankton changed in 2002 inrelation to years ago( before shrimp culture, 2000 year and 2001 year) specially in Gwatar Gulf. Mainly percentage of zooplankton were copepoda and thintinida respectively.Diversity zooplankton decreased ratio to years ago. In drainage channel, abundance total of phytoplankton and zooplankton were more than Gwatar Gulf and supply channel.
    Keywords: Aquaculture ; Iran ; Sistan and Blouchestan ; Gwatar ; Chabahar ; Environmental effect ; Shrimp culture ; Survey ; Sampling ; Temperature ; Salinity ; Dissolved oxygen ; pH ; Chlorophyll a ; Phytoplankton ; Zooplankton ; Diatoma ; Cyanophyta ; Chlorophyta ; Copepoda
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  • 3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25145 | 18721 | 2018-08-26 13:53:20 | 25145 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: A survey on Parasitic infections on endemic fishes of North of west Azarbaijan province were done during July 2001 Oct 2002. Altogether 267 specimens from 11 different fish species were examined. The fishes were collected from seven stations of Aras, Zangbar and Ghotor Rivers. The fishes were transported alive to the laboratory where they were meaursed and weighted. Then they were killed by cutting the spinal cord.. According to the results of this study 28 different parasites were revealed, 23 of them were detected to the species level and 5 of them to the genus level. The metazoans are as follows: The monogeneans, are Dactylogyrus extensus , D. chramuli, D. lenkorani, D. kendalanicus, Silurodiscoides siluri, Diplozoon megan and Gyrodoctilus sp. Two digeneans :Diplostomum spathaceum , Allocreadium ispoporum. Three Cestods: Ligula intestinalis, Digrama sp. and Caryophylleous laticeps. Two nematods: Rhabdochona hellichi, Eustrongylides excisus and only one acanthocephalus, Neoechinorhynchus rutili . The Protozoans are: Ichtyophthirius multifilis, Trichodina perforata, Chilodonella sp., Amphileptus branchiarum, Apiosoma sp/. Vorticella sp/. Myxobolus musayevi, M. cristatus. And two protozans from freshwater fishes: Stylonchia pustulata. and Arcella vulgaris. According the results of this study five new parasites and six new hosts for Iranian. Freshwater fishes were introduced. Diplozoon megan from: Abramis brama, Carassius carassius, Capoeta capoeta, Cyprinus carpio, Rutilus rutilus and Alburnoides bipunctatus. Trichodina perforata from Lucioperca lucioperca, Capoeta capoeta, Alburnoides bipunctatus, Barbus capito and Silurus glanis. Myxobolus cristatus, Tetrahymena pyriformis and Amphileptus branchiarum from Capoeta capoeta. Finally , Stylonchia pustulata and Arcella vulgaris for the first time were recoreded from freshwaters of Iran.
    Keywords: Health ; Iran ; West Azerbaijan province ; Aras River ; Zangbar ; Ghotor River ; Survey ; Specimens ; Species ; Genus ; Parasite ; Dactylogyrus extensus ; D. chramuli ; D. lenkorani ; D. kendalanicus ; Silurodiscoides siluri ; Diplozoon megan ; Gyrodoctilus sp. ; Allocreadium ispoporum ; Digrama sp. ; Rhabdochona hellichi ; Eustrongylides excisus
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25150 | 18721 | 2018-08-26 16:58:21 | 25150 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This Research has been done monthly in Sefidroud River and its tributaries from Agust 2003 to July 2004. From the total number of 2027 specimens of caught fish, 800 and 629 were males and females respectively, and 598 specimens were undistinguished. Samples were caught by cast nets. Fork length range of all specimen was 33-255 mm (119.56 ±46) and their weight rang was 0.42-257.5 gr (34.4 ±39.06) gr and their age range was 0-5 (1.76 ±0.959) year. The average of relative frequency in C.c.gracilis was 65.1 % . The average of condition factor was 1.07±0.13. Between Fork length and weight of C.c.gracilis. Was exponential relation and coefficient of correlation was estimated 99.11%. Growth cofficient (k) was 0.114 ; L_∞ was estimated 373.5 mm. C.c.g consumed of Chrysophyta phylum more than other Phytoplankton phyla in Sefidroud river and from Bentic point of view, consumed of clay, Filamentous algae detritus, insects, seeds. Absolute fecundity range was 1823-9274 (4553.8 ±2169.1) eggs. Considering the GSI and frequency percent of maturity stages, spawning period of the fish begins from April and peak of spawning is in June and terminates in July.
    Keywords: Biology ; Iran ; Sefidroud River ; Survey ; Capoeta capoeta gracilis ; Aquaculturize ; Species ; Specimens ; Males ; Females ; Fish ; Samples ; Weight ; C.c.gracilis ; Growth ; Chrysophyta phylum ; Phytoplankton phyla ; Filamentous algae detritus ; GSI ; Maturity ; Spawning
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  • 5
    Publication Date: 2021-07-16
    Description: The survey effect of salinity, turbidity and dissolved oxygen performed on Rutilus frisii Kutum juveniles with aim, which was determined about survival rate and histological changes in gill and kidney tissues. Juveniles provided from the Rajai's fish propagation center and then examined in Caspian Sea Ecology Research Center laboratory. The juveniles were investigated on three weight group (200-〈400, 400-〈600, 600-〈1000 mg) and two dissolved oxygen levels (7.6 ± 0.19, 3.8 ± 0.15 mg/L)in fresh water and Caspian sea water(12.5 ppt). Also, six turbidity levels (50 FTU, 430FTU, 2600 FTU, 7800 FTU, 15600 FTU) were probed in freshwater during 168hr. Each treatment has three replications. The factorial test evaluated for survival rate in treatments. The results shown that there was a significant difference among treatments in the different levels of factors, (p〈0.05, Duncan test). The juveniles' survival rate has decreased than control treatment in brackish water and turbidity in freshwater. It was demonstrated that with increasing fish weight, therefore, the survival rate is increased. The lowest survival rates under salinity stress (〉75%) had shown in 400-〈600mg weight group with dissolve oxygen: 3.8±0.15SE mg/l. In addition, the lowest survival rates under turbidity stress (95.83%) had shown that in weight group less than 400 mg with turbidity 15600 FTU. Therefore, survival of juveniles was high. However, gill tissue of juveniles under turbidity and salinity stress became short (necrosis) and thick (edema and hyperplasia) in the end of experiments. The juveniles gill tissues were not changes in the river clear freshwater. Therefore, the factors of gill tissue deformation were salinity and suspended particles in the water. However, the structures of kidney in the different groups of juveniles were similar. Nevertheless, juveniles glomerular diameter increased with increasing weight (p〈0.05, Duncan test). Furthermore, there was a increasing the internal cavity of the proximal and distal tubules and decreasing of glomeruli in diameter in the transfer of juveniles from freshwater to brackish water. Although the survival rate of juveniles is acceptable in this study, but there was abnormal changes in the structure of them gills. It appears that this change will cause a disruption in trend of juvenile's growth.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus frisii Kutum ; Juvenile ; Salinity ; Turbidity ; Oxygen ; Survey ; Survival rate ; Gill tissue ; Freshwater ; Brackishwater
    Repository Name: AquaDocs
    Type: monograph
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25174 | 18721 | 2018-09-03 17:15:43 | 25174 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.
    Keywords: Biology ; Iran ; Caspian Sea ; Guilan Province ; Golestan province ; Sepidrud River ; Assessment ; Sturgeons ; Population ; Genetic ; PCR-RFLP ; Microsatellite ; Species ; Samples ; Tissue ; Acipenser persicus ; A. gueldenstaedtii ; A. stellatus ; A. nudiventris ; Huso huso ; Survey
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  • 7
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
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  • 8
    Publication Date: 2021-07-16
    Description: Survey is conducted to evaluate the effects of different intensities and durations of red light spectrums on growth performance and survival of Caspian trout (Salmo trutta caspius) in Coldwater Fishes Research Center (CFRC). According to the standard methods and manuals, selected 1.1±0.2 g (1755 individuals) larvae transferred from a registered and well-known hatchery and introduced to the fish tanks in CFRC after quarantine and adaptation procedures. The trial designed for 9 treatments with 3 repeats and fish tanks (California troughs) stocked by 65 larvae. All tanks were separated by dark plastic sheets individually to more controlling actions on special lighting regimes, except controls. Lighting actions were conducted as long as survey stage for 36 days by economic lamps (P: 40/50 W, =550/880 lum) on lighting durations of 12/12, 10/14, 14/10 h (on/off) and 24 (on) by 40/50 W lamps respectively for treatment 1 to 8. Controls are considered in treatment No. 9 by natural and environmental lighting regime. The biometry operations were carried out in a 6 days interval. Results were analyzed with SPSS (Version 12) software and by ANOVA one way analysis method and multiple levels of Duncan Test; graphs were illustrated by Excel software. According to the results, most of the measured factors as growth indicators (FW, WG, FCR and SGR) in treatments (1-8) had showed significant differences in compare of control. In this case, oily drips in retina layer of eye s larvae absorb the short light spectrums and let the long spectrums to be transferred. Thus because of more vision, feeding behavior could develop with lower stress condition. Moreover, some growth factor (SGR, WG and FCR) rates in larvae showed better values significantly in treatment (1) (12/12 h lighting with 550 lum) in compare of other treatments and controls (P≤ 0.05). In addition, there are not any harmonized and expected growth parameters with synchronized red lighting intensities and durations in the Caspian trout larvae. It seems that recent demonstrations were influenced by physiological effects of some endocrinal glands and brain neurotransmitters.
    Keywords: Aquaculture ; Iran ; Caspian trout ; Salmo trutta caspius ; Larvae ; Red light ; Light strengthen ; Light duration ; Growth ; Survey ; Survival ; Coldwater ; Feeding
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25200 | 18721 | 2018-09-03 17:38:28 | 25200 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Anzali wetland at south west of Caspian located along of North West and southeast and with its unique feature were add on the Ramsar convention list in 1975. There were recorded a high rich of fauna and flora including 276 plant species, 23 reptiles and amphibians, 60 fishes, 26 mammals and 195 birds. Anzali wetland were encountered with numerous disorder with the eutrophication and the early dismissing. The first studies on Anzali wetland started by many international institutions in 1964. In this study the data of biotic and abiotic factors in Anzali wetland analyzed which belongs to different sites (15 to 38 stations) during 1991 to 2000 and were sampled seasonally or monthly in different years. The results showed the increasing trend of phytoplankton abundance from 7.9 to 66 million C./l. and the western part had the most abundant. The Cyanophyta had the most development than other 7 phylum observed in the area. In Anzali wetland identified 143 phytoplankton genus that 9 genus dominated in more than 80 % replicates and 7 genus had the abundance of more than 5 million cell/l. The Zooplankton abundant increased from 580 to 2200 ind./l. The Rotatoria had the most abundant followed by Copepoda. There was identified 104 zooplankton genus that 5 genus were dominated in more than 80 % replicates and 11 genus had the abundance of more than 30 ind./L. The chlorophyll a varied from 8.8 to 50.2 μg/l. and had a increasing trend after 1996, meanwhile it was measured higher than 25 μg/l in most of the stations. The results of benthic study showed that the Chironomidae and Tubificidae were observed more than other organisms which comprised 12.2 to 23.5% of total biomass of benthic organisms in studied period. The range of biomass was 1.1 to 7.8 g/m2. The ichthyologic survey showed presence of 60 species that 11 species were exotic. 28 species belong to freshwater, 17 species were anadromous and 8 species were resident in estuary. The highest and lowest fish catch were 640 and 288 ton in 1994 and 1999 respectively and it was about 500 ton at the end of period. The hydrochemical factors had the various fluctuation so that the nutrients parameters had a significant difference over studied period. Total nitrogen was varied from 0.78 to 1.41 mg/l and total phosphorus varied from 0.05 to 0.1 μg/l.. The oxygen dissolved amount was from 4.6 to 8.7. EC was between 3 to 5 ms/cm during September and October that indicated of Caspian water projection. According to geographic position of station, all biotic and abiotic data, were arranged. The data bank is used by Idrisi software to produce of necessary maps. Analysis of data showed that there must be a responsible department with a vast authority. Conservation and eutrophication prevention of Anzali wetland connect to special attention to catchment area and landuse management of the wetland. Also there is an urgent need for well-developed technologies and managements to reduce the organic pollution and its environmental impacts on the Anzali Lagoon.
    Keywords: Limnology ; Iran ; Caspian Sea ; Anzali wetland ; Ramsar ; Anzali Lagoon ; Limnological ; Survey ; GIS ; Fauna ; Flora ; Cyanophyta ; Phylum ; Phytoplankton ; Zooplankton ; Chlorophyll a ; Biomass ; Species ; Hydrochemical ; Nutrients ; Dissolved oxygen
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25201 | 18721 | 2018-09-05 16:03:37 | 25201 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Use and enrichment of live food resource in fish farms have been interested and highly demanded. Crustacean are one of the important groups. The Pontogammarus maeoticus dominated in southern Caspian Sea shore with a high abundance. This study was designed in order to adaptation and usage of amphipoda in fish culture ponds. The first part have been surveyed the laboratory experiments including of; to increasing and developing of P.maeoticus in 200 litter container, the effects of salinity on growth and survival of amphipods in many aquariums, the culture of common carp with amphipods and growth determination of them. Chemical composition analysis of P.maeoticus and carps fed by amphipods in compare to cultured carps from ordinary ponds. Due to concern about common healthy the heavy metal concentration has been measured in P. maeoticus, carp which were fed by amphipods and the cultured carps in earth ponds. In second phase; the adaptation of two amphipods species, P. maeoticus and Obesogammarus acuminatus was studied in fish ponds where some cages with sandy soft substrate had been provided for amphipoda replacement. Also a small surface of ponds surrounded by net and covered by Azola plant, a habitat suitable for to putting of O. acuminatus. Production of amphipoda had not the successfully results in large tanks. Aquariums with Caspian Sea water had the prosper results where the specimens were breeding and developing properly, even though in some aquarium with freshwater increased the amphipods number. The chemical composition had not significant difference between two kind of cultured carps while the organic component in amphipoda had a high quality. The better quality of cultured carp by amphipod diet have been confirmed by organoleptic test. The results of heavy metal measurement in amphipoda showed a high concentration which some of them were transmitted to cultured carps. Result of amphipoda replacement in cage was not satisfy and the specimens were died after some days. According to hydro-chemical parameters the oxygen poorness and high trophy levels were the affective factor to abolish of specimens in cages. It seems that there are many type of P.maeoticus that can be adapted in different salinities. The molecular differentiation should be investigated to choose the suitable type of this spices for utilization in freshwater fish ponds. In other hand it can be used in fish culture ponds that will be supplied by brackish water.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Adaptation ; Amphipoda ; Fish ; Culture ; Ponds ; Enrichment ; Pontogammarus maeoticus ; Amphipoda ; Survey ; P.maeoticus ; Common carp ; O. acuminatus ; Oxygen ; Specimens ; Brackish water
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