ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Genetics
  • Wiley-Blackwell  (34)
  • Blackwell Publishing Ltd
  • 1985-1989
  • 1980-1984  (34)
  • 1925-1929
  • 1981  (34)
  • 1
    Electronic Resource
    Electronic Resource
    Masthead (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020101
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Haltere determination and mutations at the bithorax locus (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 49-73 
    Details
    ISSN: 0192-253X
    Keywords: determination ; Drosophila ; haltere disc ; homeotic mutation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Mutations at the bithorax locus transform anterior haltere tissue into anterior wing. These transformations could in principle be due to the mutations altering either the expression or cell heredity functions of determination. I have studied two alleles of the bithorax locus bx3 and bx34e using disc culture techniques and found that both produce their transformations by altering the expression of the determined state. I have also found that the expression of the temperature-sensitive allele, bx34e, can be altered by temperature shifts during the culture period. Evidence has been obtained that suggests that such changes in expression do not require growth or cell division.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020106
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The immaturity interval in Tetrahymena: Genetic and environmental sources of variation (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 159-170 
    Details
    ISSN: 0192-253X
    Keywords: Tetrahymena hegewischi ; timing of maturity ; cellular differentiation ; genetic ; environmental variation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The development of sexual maturity has been studied in Tetrahymena hegewischi. Progeny lines do not typically change from immaturity to mating with all different mating types during a single test interval, but about 30% do mature abruptly. Some testers are more likely than others to participate in the earliest mating reactions of progeny lines which do not mature abruptly. Subcaryonidal vegetative pedigrees of 10 pairs from 4 crosses revealed considerable intrapair variation in the time, measured in fissions, of maturity. The average intrapair coefficient of variation was 20%. A nested ANOVA revealed significant genomic effects on the immaturity interval, but no significant cytoplasmic or caryonidal effects; 56% of the total variation was non-genomic. Growth in different environments had highly significant effects on the immaturity interval. Subclones grown at 27°C with alternate day transfers took on the average 2 to 3 times as many fissions to mature as sister subclones grown at 27°C with daily transfers. Subclones grown at 18°C or 34°C and transferred on alternate days had intermediate maturation times. The greatest range in the immaturity interval among lines of the same genotype was from 34 to 143 fissions. The development of maturity in this species involves genetic control of timing, but the genetic differences are obscured by a large amount of intraclonal variation and sensitivity to the environment.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020203
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Masthead (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020301
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Organization of mitochondrial DNA in normal and texas male sterile cytoplasms of maize (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 319-336 
    Details
    ISSN: 0192-253X
    Keywords: maize ; mitochondrial DNA ; recombinant DNA ; cms-T ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Recombinant DNA and hybridization techniques have been used to compare the organization of mitochondrial DNA (mtDNA) from normal (N) and Texas male sterile (T) cytoplasms of maize. Bam H1 restriction fragments of normal mtDNA were cloned and used in molecular hybridizations against Southern blots of Bam H1 digested N and T mtDNA. Fifteen of the 35 fragments were conserved in both N and T as indicated by hybridization to comigrating bands in their restriction patterns. Only three fragments produced autoradiographs whose differences could reasonably be attributed to single changes in the cleavage site of the enzyme while approximately half (17/35) of the clones resulted in more complicated differences between N and T. The autoradiographs produced by these 17 clones indicated multiple cleavage site changes and/or sequence rearrangements of the mtDNA. Patterns of six of these 17 clones indicated partial duplication of the sequence and two showed variation in the intensity of hybridization between N and T, which may be related to the molecular heterogeneity phenomenon found in maize mitochondrial genomes. The large proportion of changes observed between N and T mtDNA indicates that rearrangements may have played an important role in the evolution of the maize mitochondrial genome.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020402
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Triploidy permits survival of an inviable amphibian hybrid (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 357-367 
    Details
    ISSN: 0192-253X
    Keywords: amphibian hybrids ; exogastrulation ; hybrid lethality ; nucleocytoplasmic interactions ; triploidy ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Hybrids between species frequently arrest early in development. In the frog hybrid Rana catesbeiana female × Rana clamitans male, the embryo shows a characteristic development to an exogastrula which dies. This hybrid can be rescued by pressure suppression of the second polar body, which results in the addition of another haploid set of R catesbeiana chromosomes to the embryo. The triploid hybrid expresses genes from both species and can develop normally through metamorphosis. The results show that an R catesbeiana egg containing a full haploid set of R clamitans chromosomes is capable of development and that the usual developmental arrest caused by the R clamitans genome responds to chromosomal dosage.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020405
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Probable somatic DNA rearrangements in mating type determination in Tetrahymena thermophila: A review and a model (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 185-202 
    Details
    ISSN: 0192-253X
    Keywords: somatic DNA alteration ; nuclear differentiation ; mating types ; ciliate genetics ; immunoglobulin genes ; Tetrahymena thermophila ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Experimental data on mating type determination in T. thermophila, collected by Nanney, Allen, and their collaborators over a period of 25 years, are reinterpreted in the light of our current understanding of macronuclear genetics. A strong case is developed supporting the idea that mating type determination involves the developmental alteration of somatic DNA that occurs regularly in developing macronuclei in conjugating pairs. A. testable DNA deletion/splicing model is developed that although based on a few simple, plausible assumptions, explains the observations remarkably well. The model is in (at least) superficial analogy to the mechanism that must be involved to explain the somatic differentiation and alteration of DNA sequences that ultimately constitute an expressed vertebrate immunoglobulin gene. Because of the genetic, biochemical, and micromanipulative versatility of Tetrahymena, it may well turn out to be a uniquely suitable microbial eukaryotic experimental system for the study of developmental alterations of somatic DNA.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020205
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    The expression of β-galactosidase during preimplantation mouse embryogenesis (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 1-12 
    Details
    ISSN: 0192-253X
    Keywords: β-galactosidase ; preimplantation ; mouse ; Bgl ; paternal effect ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Lysosomal acid hydrolase expression during preimplantation mouse embryogenesis has proved useful in estimating when mRNA transcription commences during this period. Previous work from this laboratory has shown that α-galactosidase and β-glucuronidase undergo 50- to 100-fold increases in activity between the two-cell stage and the blastocyst stage [1, 2]. Here we show that β-galactosidase activity levels undergo a similar change. We also demonstrate that mouse strains with the Bgl-sh allele produce cleavage stage embryos with 2-4-fold higher activity levels than strains with the Bgl-sd allele. Bgl has been shown to control β-galactosidase levels in adult mouse tissues [3]. Unfertilized egg β-galactosidase levels are also regulated by Bgl, but loci distant from Bgl modify egg expression. The distant sites are not observed to act during cleavage. Hybrid embryos (Bgl-sd/h) show intermediate activity levels to the parental types. The timing of the deviation of hybrid embryo β-galactosidase activity levels from maternal-type activity levels is used to estimate when transcription of genes governing β-galactosidase expression occurs.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020102
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    A mutation with pleiotropic effects on macronuclearly differentiated functions in Paramecium tetraurelia (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 23-34 
    Details
    ISSN: 0192-253X
    Keywords: Paramecium tetraurelia ; trichocysts, nuclear differentiation ; cytoplasmic inheritance ; determination ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The mtFE mutation isolated in Paramecium tetraurelia affects mating type differentiation, trichocyst excretion, and viability. Its effect on mating type has already been shown to correspond to a restriction to the E mating type interpreted by an inefficiency of nuclear O-determining factors. In this paper we study the other two phenotypic characteristics whose hereditary transmission displays two unusual features. (1) In crosses between a wild-type strain and the mutant strain, the mutant characteristics do not reappear in F2 in the wild-type cytoplasmic lineage but only in F3 after the homozygous clones have undergone an additional nuclear reorganization. (2) Some F2 wild-type clones, in the mutant cytoplasmic lineage, retain some of the phenotypic characteristics of the mutant. We propose that the mtF gene product plays a role in the control of several macronuclearly differentiated functions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020104
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Influence of homoeotic genes on the aldehyde oxidase pattern in imaginal discs of Drosophila melanogaster (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 75-87 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila melanogaster ; homoeosis ; imaginal discs ; aldehyde oxidase ; pattern formation ; compartments ; selector genes ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In a study of the regulation of enzyme patterns in imaginal discs the aldehyde oxidase pattern was determined for some homoeotic mutations of D. melanogaster. Earlier indications that suggested that this pattern follows the determinitive state of compartments within imaginal discs were confirmed by the aldehyde oxidase (AO) pattern of both the wing and haltere discs from en1; bx3, en1; pbx, and en1; bx3 pbx larvae and the antennal discs from Antp73b and ssa larvae.We additionally analyzed whether AO activity depended on the determinative state of an entire compartment or was expressed autonomously in clones. Homozygous engrailed clones were induced by mitotic recombination. From the AO clones found in normally negative areas of the posterior compartment it was concluded that enzyme activity depended upon the determinative state of the cells and was not a function of the compartment as a whole.The results are described with reference to a scheme in which compartmental and subcompartmental selector genes are thought to determine a binary code on which AO patterns depend.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020107
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    The production of chimeric rats and their use in the analysis of the hooded pigmentation pattern (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 131-146 
    Details
    ISSN: 0192-253X
    Keywords: rat chimeras ; hooded pigmentation ; melanocyte ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: New, improved media and procedures for making rat chimeric embryos and culturing them in vitro have been developed. We have produced 27 rat chimeras: 20 males and 7 females. This ratio of males to females is consistent with that seen in mouse chimeras, suggesting that rat sex chimeras develop as phenotypic males. By aggregating embryos containing appropriate genetic markers for pigment cell differentiation, it is possible to produce chimeras that elucidate the site of action of the hooded gene. The coat color patterns of black ↔ black hooded chimeras display a white belly spot. In black ↔ albino hooded chimeras, small patches of white hair appear on the head and a large white spot occurs on the belly. Black ↔ agouti hooded chimeras display both agouti and nonagouti pigmentation over the entire surface of the chimera. These animals are fully pigmented with no white spots. In black ↔ albino non-hooded chimeras, rather small irregular patches of black and white hairs are distributed throughout the pelage. Histological examination of sections of hair follicles obtained from the white areas in the head of black ↔ albino hooded chimeras revealed amelanotic melanocytes. On the other hand, hair bulbs from the white belly spots do not contain any such melanocytes. Thus the white hairs of the head are due to the presence of albino melanocytes, but the white hairs of the belly are due to the total absence of melanocytes. All these observations are consistent with the conclusion that the hooded gene acts within melanoblasts, probably to retard their migration from the neural crest and/or to prevent their entrance into the hair follicles of the white areas of hooded rats.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020111
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Visualization of the F9 antigen on sperm from normal and T/t-complex mutants by immunoscanning electron microscopy (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 171-183 
    Details
    ISSN: 0192-253X
    Keywords: sperm ; F9 antigen ; T/t-complex ; immunolabeling ; scanning electron microscopy ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The antigens defined by conventional syngeneic antiserum against F9 embryonal carcinoma cells were localized on mature sperm using immunolabeling and scanning electron microscopy. Labeling patterns were compared for normal (+ / +) mice and mice bearing recessive t-haplotypes. The results showed that antigens detected by intact anti-F9 antiserum are expressed similarly in all genotypes, except for sperm from mice bearing the t12-haplotype where the frequency of labeled cells was reduced. Labeling with the IgM fraction of anti-F9 antiserum was lower on sperm from all t-genotypes examined, with sperm from + /t12 males showing the most marked reduction. In all cases, the labeling patterns were similar, and included a labeling of the whole sperm head with complete anti-F9 antiserum and a restriction of the label to the postacrosomal region when the IgM fraction was used.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020204
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Announcements (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 219-222 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020207
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Revertants of selfing (gad) mutants in Physarum polycephalum (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 253-267 
    Details
    ISSN: 0192-253X
    Keywords: myxomycete ; Physarum polycephalum ; mating type ; sexual compatibility ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The conversion of the uninucleate amoebal form of Physarum polycephalum to the multi-nucleate plasmodial form is under the control of a genetic region which contains matA (or mt), a determinant of mating specificity. The region is the site of most gad mutations, which give amoebae the ability to produce plasmodia in clones without mating (ie, to self). In the present study, nonselfing revertants were isolated from two matA2-derived gad mutants and two matA3-derived gad mutants. Some revertants were found to have regained exactly, or nearly, the same phenotype as the original matA2 or matA3 strain. Others expressed new mating types, having gained the ability to mate with strains of the parental matA type. The results are compatible with a model in which new mating types arise from forward mutations (gad) and back mutations (npf or no plasmodium formation) occurring successively in a single gene, matA.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020304
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    The developmental appearance of androgen receptor protein and androgen inducibility of the gus gene complex in mouse kidney (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 269-277 
    Details
    ISSN: 0192-253X
    Keywords: β-glucuronidase ; androgen ; receptor ; development ; mouse ; kidney ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: During postnatal development of mouse kidney the androgen responsiveness of epithelial cells for β-glucuronidase induction, cellular hypertrophy, and other enzyme inductions appears coincidentally with a rise in androgen receptor protein. Initially, a low level of receptor is present but no response is seen. Beginning at about 12 days of age responsiveness begins to increase, reaches a half-maximal level at 18-20 days, and full responsiveness by 28-30 days. The limiting factor appears to be levels of androgen receptor protein.Our experiments shed no light on the question of why each androgen responsive cell type in the organism differentiates the capacity to induce a different array of proteins. However, they do suggest that responsiveness of the β-glucuronidase gene does not appear until a minimum threshold level of receptor is exceeded, and that the response of the gene may not be saturated even at the highest levels of receptor reached.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020305
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Non-random X-chromosome expression early in mouse development (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 305-315 
    Details
    ISSN: 0192-253X
    Keywords: X-chromosome inactivation ; mouse ; PGK-1 ; embryonic cell lineage ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have used a sensitive electrophoretic technique for estimating the activity, or ratio, of two allozymes of the X-chromosome-linked enzyme phosphoglycerate kinase (PGK-1), in order to investigate the randomness of X-chromosome expression in the derivatives of the three primary cell lineages of the early mouse conceptus. The maternally derived Pgk-1 allele is preferentially expressed in the derivatives of the primitive endoderm and trophectoderm lineages at 6 1/2 days post coitum in Pgk-1a/Pgk-1b heterozygous conceptuses, and in the one informative 5 1/2-day heterozygous conceptus analysed. This evidence for preferential expression of the maternally derived X chromosome (Xm), so soon after the time of X-chromosome inactivation, favors the possibility that the preferential expression of Xm is a consequence of primary non-random X-chromosome inactivation, rather than a secondary selection phenomenon. The majority of embryos analysed at 4 1/2 and 5 1/2 days pc produced only a single PGK-1 band, corresponding to the allozyme produced by the Pgk-1 allele on Xm, although 50% of these embryos should have been heterozygous females. Possible explanations are discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020308
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Studies of fs(1)1621, a mutation producing ovarian tumors in Drosophila melanogaster (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 203-218 
    Details
    ISSN: 0192-253X
    Keywords: cytokinesis ; fusome ; intercellular bridges ; oogenesis ; sterility ; tumor mutations ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The ethyl methane sulfonate-induced mutation, fs(1)1621, resides at 11.7 on the genetic map and within segment 4F1-5A1 of the cytological map of the X chromosome. When homozygous, fs(1)1621 renders females semisterile but has no effect on their viability; nor does it affect the viability or fertility of hemizygous males. Heterozygous females are fertile and have cytologically normal ovaries. The ovaries of homozygous females first produce normal oocytes, which, if fertilized, can develop into adult males or females. After this period, ovarian chambers containing only pseudonurse cells are formed, and finally mutant germaria produce only tumors. These contain hundreds to thousands of cells that appear to be derived from germarial cystocytes, because they occasionally form clones of interconnected cells and also can differentiate into endopolyploid pseudonurse cells. Raising the temperature speeds the rate at which tumors form; lowering it increases the probability of pseudonurse cell differentiation. Df(1)C159 includes fs(1)1621. The pattern of ovarian chamber production is more temperature sensitive in hemizygous females than in homozygous ones. The morphology of hemizygous tumors and the number of dividing cells within them also differ from homozygotes. These observations support the hypothesis that fs(1)1621 is producing a product, that less is produced by one gene than by two, and that the product plays a role in the mitosis and cytokinesis of ovarian cystocytes.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020206
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Genetic analysis of mating type differentiation in Paramecium tetraurelia. III. A mutation restricted to mating type E and affecting the determination of mating type (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 13-22 
    Details
    ISSN: 0192-253X
    Keywords: nuclear differentiation ; cytoplasmic inheritance ; Paramecium tetraurelia ; mating type ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In P. tetraurelia each cell is determined to express only one of the two complementary mating types, O and E. This determination is under cytoplasmic control and seems to be achieved only by the commitment or noncommitment to the expression of mating type E. All the previously known mutations affecting the differentiation of mating type prevent the expression of the E mating type (O-restricted mutations) without affecting the determination process. An E-restricted mutation was obtained: mtFE. Its phenotypic properties indicate that the mutation affects the determination process itself. When an O cell becomes mtFE/mtFE it acquires the E mating type and an E-determining cytoplasm. We propose that this constitutive determination for the E mating type is due to the inefficiency of a factor which is normally active in an O cell. This factor would act like a repressor and stabilize the E functions under an inactive state.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020103
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    A biochemical analysis of cartilage proteoglycan in the avian mutant micromelia-abbott (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 35-48 
    Details
    ISSN: 0192-253X
    Keywords: proteoglycan ; micromelia ; avian embryo ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Embryos homozygous for the recessive lethal gene, micromelia-Abbott, develop a severe form of micromelia, a parrot-like beak, and hemorrhagic skin. Feather development is also retarded. The reduction in length of the long bones of the leg can be traced to their cartilaginous stage Quantitative analysis of sulfated proteoglycan (PGS), a major macromolecular component of cartilage matrix, reveals that mutant tibiae, femora, and sterna contain significantly less uronic acid per μg of DNA than normal rudiments, indicating reduced accumulation of PGS in the mutant. Incorporation of radioactive precursors into cartilage PGS is severely reduced in relatively early developmental stages of a particular organ, but this reduction becomes less severe in cartilage taken from the same rudiment at a later developmental stage. Analysis of the sedimentation rate of PGS in sucrose gradients reveals no difference between normal and mutant in all cartilaginous types at all ages. These results suggest that the quantity and not the quality of PGS is affected in this mutant. The observation that the addition of para-nitrophenyl-β-D-xyloside to the culture medium can stimulate glycosaminoglycan synthesis to normal levels is interpreted to mean that the reduced levels of PGS may be the result of a reduced availability of the xylosylated protein backbone for PGS.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020105
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Genetic regulation of alcohol dehydrogenase C2 in the mouse. Developmental consequences of the temporal locus (Adh-3t) and positioning of Adh-3 on chromosome 3 (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 89-98 
    Details
    ISSN: 0192-253X
    Keywords: alcohol dehydrogenase C2 ; isozymes ; temporal locus ; genetics ; chromosome 3 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The tissue specificity of a proposed cis-acting temporal locus (Adh-3t), which regulates alcohol dehydrogenase C2 (ADH-C2) activity in mouse reproductive tissue extracts, has been examined in C5 7BL/6J, SM/J, F1 (SM/J × C5 7BL/6J) mice as well as in progeny of an (F1 [SM/J × C5 7BL/6J] × C5 7BL/6J) back-cross. Electrophoretic variants for ADH-C2, previously used to localize the gene (Adh-3) encoding this enzyme on chromosome 3, enabled the relative parental contributions to ADH-C2 phenotype in F1 and backcross mouse tissues to be determined. These analyses demonstrated that (1) stomach, kidney, lung, adrenals, seminal vesicles, epididymis, uterus, and ovary ADH-C2 is encoded by a single locus (Adh-3); Adh-3t is differentially active in various tissues, eg, lung exhibits no apparent activity whereas the temporal locus is fully active in seminal vesicles; (3) Adh-3t is probably differentically active in different cells of some tissues, eg, adrenals. Specific activity profiles of stomach and epididymal ADH-C2 during the neonatal development of C5 7BL/6J, SM/J, and F1 (SM/J × C5 7BL/6J) male mice supported the proposal for a cis-acting temporal locus for this enzyme. Genetic analyses examining segregation of Adh-3 and Adh-3t among backcross progeny suggested that these are distinct but closely linked loci, since one recombinant among 256 progeny was observed. Linkage data of Adh-3 with Va (varitint-waddler) and de (droopy ear) was also obtained, which suggested that Adh-3 is localized on chromosome 3 between Va and de.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020108
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Inhibition by CIPC of mitosis and development in Dictyostelium discoideum and the isolation of CIPC-resistant mutants (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 99-111 
    Details
    ISSN: 0192-253X
    Keywords: CIPC as a mitotic inhibitor of Dictyostelium ; inhibition of Dictyostelium development by CIPC ; CIPC-resistant mutants ; Dictyostelium ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The anti-mitotic herbicide isopropyl N-(3-chlorophenyl) carbamate (CIPC) prevents the growth of amoebae of Dictyostelium discoideum without killing the cells for a period of time equivalent to one generation. During in-hibition, amoebae accumulate in prophase and metaphase of mitosis. After removal of CIPC, they continue through mitosis and then divide.The addition of CIPC to amoebae under starvation conditions prevents aggregation and concomitant cell elongation. The cells, however, do not lose their ability to adhere to a surface, and they remain viable. When CIPC is added to amoebae which have formed streams, it leads to the disintegration of streams into small clusters of cells and to a loss of cell elongation.Post-aggregation stages of development can be inhibited by CIPC at the mound, slug, or Mexican hat stages. Slugs break apart into distinct aggregates.Mutants resistant to CIPC can be obtained easily. Among these mutants, many become temperature sensititive for growth (27°C) or development (27°C or 15.5°C). Others show various abnormalities at the normal temperature (22°C). Most mutants are cross resistant to the microtubule inhibitors nocodazole and thiabendazole, and some are also resistant to CIPC during development.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020109
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Genetic control of the developmental program of L-glycerol-3-phosphate dehydrogenase isozymes in Drosophila melanogaster: Identification of a cis-acting temporal element affecting GPDH-3 expression (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 113-129 
    Details
    ISSN: 0192-253X
    Keywords: temporal genes ; GPDH isozymes ; regulation ; development, Drosophila ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The complete developmental program of glycerol-3-phosphate dehydrogenase in wild type Drosophila is described with respect to activity, isozyme expression, and GPDH-specific CRM. Variants of this developmental program have been isolated from natural populations which affect the rate of accumulation of only the GPDH-3 isozyme in both the larval and adult stages of development. This activity variation segregates as a single gene which is tightly linked to the structural element on Chromosome II, exhibits cis-control, and is tissue specific in expression. This gene meets all the criteria for temporal regulatory genes.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020110
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    Masthead (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020201
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    The genetics of dopa decarboxylase in Drosophila melanogaster. III. Effects of a temperature sensitive dopa decarboxylase deficient mutation on female fertility (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 223-235 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila ; dopa decarboxylase ; female fertility ; temperature sensitive lethal mutant ; differential tissue-specific responses to temperature ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The mutation Ddcts1 effects female sterility when homozygous, hemizygous, or heterozygous over a series of Ddc null alleles (Ddcx) indicating that some aspect of Ddc gene function is necessary for female fertility. Ovary transplant experiments demonstrate that the female sterility phenotype is ovary autonomous. Two to 3% of the total DDC activity measurable in newly hatched females is localized in their previtellogenic ovaries. The degree to which females heterozygous for Ddcts1 over different Ddc null alleles are fertile at 22°C reflects a continuous spectrum of allelic complementation similar to that observed for the effects of these genotypes on viability at 30°C. Fertility of all the Ddcts1/Ddcx females tested is significantly depressed at 30 vis-a-vis 22°C providing evidence that it is the DDC enzyme activity itself which is required for female fertility. Ddcts1/Ddcts1 homozygous and Ddcts1/Df hemizygous females are nonconditionally, completely sterile at 18, 20, 22, 25, and 30°C. Although all homo- and hemizygous females do lay some eggs, no evidence of embryogenesis or fertilization has ever been detected. The absolute, nonconditional sterility of Ddcts1 homo- and hemizygous females stands in stark contrast to the conventional temperature dependent effects of these same genotypes on viability and to the temperature sensitive effects of Ddcts1/Ddcx heterozygous females on both fertility and viability. Reasons for these tissue-specific and genotypic differences are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020302
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    Developmental controls of morphological mutants of Phaseolus vulgaris L.: Differential expression of mutant loci in plant organs (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 279-290 
    Details
    ISSN: 0192-253X
    Keywords: temperature-sensitive mutant ; cytokinin ; hormonal metabolism ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Developmental controls of morphological mutants of Phaseolus vulgaris L. conditioned by two independent loci, DL1 and DL2, were examined through grafting experiments and hydroponic studies. Phenotypes of mutant classes were duplicated by unions of scions and stocks derived from different genotypes. Results indicate that DL1 and DL2 regulate a root and shoot factor respectively, contributing to the mutant types. The allelic dosages of DL1 in the root and DL2 in the shoot rather than the genotype of the whole plant per se determine the severity of the mutant expression. Plants heterozygous for both loci with a temperature-sensitive expression of the mutant phenotype were used to determine physiological components involved. The primary abnormal developmental event associated with the appearance of mutant phenotypes, the restricted root growth at high temperature, could be overcome by the addition of cytokinin in hydroponic solution. These observations suggest that DL1 and DL2 may be related to the regulation of hormonal function or metabolism.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020306
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Masthead (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020401
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Endogenous formation of prostanoids and the levels of long-chain fatty acyl CoA derivatives in suspensive and adhesive cells from in vitro embryoid bodies of teratoma OTT 6050 (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 337-347 
    Details
    ISSN: 0192-253X
    Keywords: suspensive and adhesive teratoma cells ; teratoma embryoid bodies ; cell differentiation ; endogenous prostanoid biosynthesis ; long-chain fatty acyl CoA derivatives ; mass fragmentography ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Attachment of the cell surface to a substratum may play a critical role in initiating some cellular developmental commitments and in sustaining differentiation of cells that have already been specialized. Embryoid bodies of teratoma OTT6050 were divided, on day 10 of initial culture, into myogenic adhesive cells which were already (at day 6) characterized by endogenous prostaglandin (PG)I2 formation and little-specialized suspensive cells which formed only thromboxane (TX)B2 in the same culture system. Since at day 10 both cell types reached a stationary phase in which the nature of each cell was mature enough for the analyses with mass fragmentographic technique and gas chromatography- mass spectrometry (GCMS), the total levels of predominant long-chain fatty acyl CoA (acyl CoA) derivatives could be measured comparatively as methyl esters after methanolysis. It was found as a result of major differentiation that adhesive cells had a rather low ratio of arachidonyl CoA to stearyl CoA, although adhesive cells accumulated a larger total amount of acyl CoA derivatives than that accumulated in suspensive cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020403
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    Intracellular cAMP in Dictyostelium discoideum: Characterization of an aggregation-deficient mutant with elevated cAMP pools (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 369-383 
    Details
    ISSN: 0192-253X
    Keywords: Dictyostelium discoideum ; aggregation-deficient mutants ; intracellular cAMP ; adenylate cyclase ; defective spore differentiation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Forty aggregation-deficient mutants of Dictyostelium discoideum were screened for changes in intracellular cAMP during the first 10 hr of starvation. The pools in 39 of the mutants remained low and relatively static during this period. However, amoebae of one mutant, strain HC151, exhibited significantly elevated levels of intracellular cAMP during vegetative growth and for several hours after starvation. A more detailed analysis of this mutant indicated that the elevated cAMP pools in these cells are a consequence of the premature appearance and partial activation of an adenylate cyclase. The mutation(s) altering adenylate cyclase regulation in this strain appears to map in linkage group IV. Complementation tests between strain HC151 and another mutant, HH201, which has recently been shown to produce an adenylate cyclase activity precociously [1], indicated that the mutations affecting adenylate cyclase activity in these strains map at different loci. Although both of these mutations behave recessively in heterozygous diploids with respect to gross development, an examination of early cAMP metabolism and terminal spore differentiation in these diploids suggest that these mutations are at least partially expressed during some stage(s) of the developmental cycle.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020406
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    An X chromosome locus in Drosophila melanogaster that enhances survival of the triplo-lethal genotype, Dp-(Tpl) (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 147-158 
    Details
    ISSN: 0192-253X
    Keywords: triplo-lethal locus ; Tpl ; gene-dosage ; X chromosome ; Drosophila melanogaster ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Only a single locus (Tpl) is known in the Drosophila melanogaster genome that leads to early lethality when present as a heterozygous duplication (three doses) or deficiency (one dose). We report the recovery of third instar larvae (and of occasional adults) carrying a duplication for the triplo-lethal locus, Dp(Tpl). Karyotype analysis of the larvae showed that the individuals surviving were almost entirely 3X;2A metafemales. We examined the question of whether the entire X or a single X locus was a major factor permitting survival. X-Y translocations were used to produce females hyperploid for different portions of the X and carrying Dp(Tpl). Analysis of metaphase chromosomes by quinacrine fluorescence pattern indicates that the X chromosome region between 6D and 7DE must be present in an extra copy to enhance the survival of Tpl duplication-bearing females. Another type of experiment suggests that it is the region between 7C and 7DE which is essential.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020202
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    Developmental modulation of protein synthesis in Drosophila primary embryonic cell cultures (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 237-252 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila ; embryonic cultures ; protein synthesis ; stage specific ; differentiation ; two-dimensional gel electrophoresis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The patterns of proteins synthesized during embryonic development in Drosophila melanogaster have been examined by two-dimensional gel electrophoresis. Primary cell cultures prepared from donor embryos synchronized to ± 1 hr were labeled with [35S]methionine at 5, 11.5, 14.5, and 26 hr after oviposition. Of approximately 400 to 500 proteins detected, the synthesis of about 50 is developmentally modulated. The greatest number of changes in the synthesis of stage-specific proteins occurs at 11.5 and 14.5 hr after oviposition, periods just prior to and during the times of the greatest overt morphological and biochemical changes. At 11.5 hr, 35 stage-specific proteins are synthesized, including 19 that are not present at the previous stage examined. At 14.5 hr, 34 stage-specific proteins can be detected, including 11 newly synthesized proteins. However, 12 proteins from the previous stage are no longer synthesized. At the completion of embryonic differentiation, at 26 hr, no new proteins are synthesized and the synthesis of many present in earlier stages has decreased or stopped. Comparison of patterns of embryonic proteins to those synthesized by two Drosophila continuous cell lines reveals that the majority of proteins are common to all. However, only about 40% of the embryonic stage-specific proteins are present in either cell line. In addition, there are several proteins unique to each cell line that are not observed in any of the embryonic stages.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020303
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    Announcing (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 317-317 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020309
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    Enzyme patterns in trisomy 19 of the mouse (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 291-303 
    Details
    ISSN: 0192-253X
    Keywords: mouse ; trisomy ; gene dosage ; enzyme activity pattern ; phosphoglycerate mutase (PGAM) ; glutamate oxaloacetate transaminase (GOT) ; isozyme ; developmental pattern ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Activity patterns of cytosolic and mitochondrial enzymes of carbohydrate and amino acid metabolism have been measured in murine trisomy 19. In spite of marked hypoplasia, no significant alterations of the patterns (per gram of organ weight) were observed, with the exception of glutamate oxaloacetate transaminase (GOT-1), and phosphoglycerate mutase (PGAM). Clear-cut gene dosage effects in liver, brain, heart, skeletal muscle, and erythrocytes of fetal and newborn mice, confirm the assignment of GOT-1 to chromosome 19. Data obtained for PGAM demonstrate that one of the two different subunits leading to organ-specific isozyme patterns of the dimer enzyme protein is coded on chromosome 19 (gene Pgam-1). Dosage effects are fully expressed in liver, brain, and erythrocytes (AA-type isozyme), but not in skeletal muscle (BB-type isozyme). Dosage effects on the hybrid AA-AB-BB-isozyme pattern in the course of development of the heart muscle, were demonstrated by means of quantitative activity measurement after electrophoretic separation. The comparison of enzyme patterns of eusomic and trisomic erythrocytes, produced after injection of fetal stem cells into irradiated adult carriers (transplantation chimaeras), revealed enzyme activity ratios that were similar to those produced by erythrocytes of adult euploid and trisomic mice. This is in agreement with the chromosome assignments and dosage effects mentioned above.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020307
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Induction of the shift in melanin synthesis in lethal yellow (Ay/a) mice in vitro (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 349-356 
    Details
    ISSN: 0192-253X
    Keywords: agouti locus ; lethal yellow gene ; MSH ; dibutyryl cyclic AMP ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Alleles at the agouti locus determines the type of pigment produced in hair-bulb melanocytes. In order to analyze the mechanism of agouti gene function, an attempt was made to induce the shift in melanin synthesis in vitro. Skin explants from newborn yellow mice with genotype Ay/a were cultured with the method using membrane-filter and roller tube. Production of black pigment in the hair bulbs was observed when the explants were cultured in the presence of α-melanocyte stimulating hormone (α-MSH). Electron-microscopic observation indicates that the induced black pigments are eumelanin that is normally found in hair-bulb melanocytes of genotypically black mice. The eumelanin synthesis was also induced by cAMP, DbcAMP, or theophylline. This α-MSH-induced eumelanin synthesis was suppressed by actinomycin D or cycloheximide, suggesting that the α-MSH-induced eumelanogenesis requires de novo transcription and/or translation.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020404
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    The analysis of mammalian development using allelic isozyme variants (1981)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 2 (1981), S. 385-405 
    Details
    ISSN: 0192-253X
    Keywords: allelic isozyme variants ; mammalian development ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The past decade has seen an explosion of interest in mammalian embryos. Techniques of molecular and genetic analysis coupled with advances in in vitro culture and experimental manipulation of mammalian embryos have provided important insights into mechanisms of embryogenesis. Many of these recent advances have been facilitated by the use of allelic isozyme variants as autonomous cell markers or representative gene products. Investigations aimed at exploring cell lineages and cell commitment, the timing and regulation of gene expression, X chromosome inactivation, and cell interactions have depended on the availability of appropriate isozyme variants. Results from such experiments are summarized here in order to demonstrate the usefulness of this approach and to stimulate its wider application in developmental biology.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020020407
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...