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  • United States  (330)
  • Protein Structure, Tertiary  (100)
  • Physics
  • American Association for the Advancement of Science (AAAS)  (433)
  • London : Springer
  • 2000-2004  (433)
  • 2001  (433)
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  • American Association for the Advancement of Science (AAAS)  (433)
  • London : Springer
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  • 2000-2004  (433)
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  • 1
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-09-07
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lawler, A -- New York, N.Y. -- Science. 1999 Dec 17;286:2251.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11542666" target="_blank"〉PubMed〈/a〉
    Keywords: National Academy of Sciences (U.S.) ; Research/organization & administration/*trends ; United States ; United States National Aeronautics and Space Administration/*organization & ; administration
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-24
    Description: Preparing for their 30th conference, neuroscientists contemplate how the field--and the annual meeting--has grown. With the Society for Neuroscience meeting routinely packing in about 25,000 people, some neuroscientists complain about long lines and related inconveniences. But others say that the best reason to go to the meeting is precisely because it's so huge and integrates the ever-growing number of subdisciplines in the field.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Helmuth, L -- New York, N.Y. -- Science. 2000 Oct 27;290(5492):698-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11184193" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Congresses as Topic ; Humans ; *Neurosciences ; *Research ; *Societies, Scientific/organization & administration ; United States
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    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-24
    Description: The U.S. Department of Agriculture requested comments earlier this year to help it decide whether to adopt a formal definition of "distress" as part of its responsibilities under the Animal Welfare Act. But the 2600 pieces of advice it received during a 4-month period that ended earlier this month suggest that its job won't be easy. The comments highlight a deep split between animal activists, who see the potential new regulations as a step toward eliminating all painful procedures, and most researchers, who say that the present system is working well and that no major changes are needed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Holden, C -- New York, N.Y. -- Science. 2000 Nov 24;290(5496):1474-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11185495" target="_blank"〉PubMed〈/a〉
    Keywords: *Animal Experimentation ; *Animal Welfare/legislation & jurisprudence ; Animals ; *Animals, Laboratory ; *Government Regulation ; Pain/veterinary ; *Research ; Stress, Physiological/*veterinary ; United States ; *United States Department of Agriculture/legislation & jurisprudence
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-24
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Balter, M -- New York, N.Y. -- Science. 2000 Oct 6;290(5489):39.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11183145" target="_blank"〉PubMed〈/a〉
    Keywords: Abortifacient Agents, Steroidal/*history ; Aging/drug effects ; Dehydroepiandrosterone/history/pharmacology ; *Drug Approval ; Drug Industry ; Female ; France ; History, 20th Century ; Humans ; Mifepristone/*history ; United States ; United States Food and Drug Administration
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-24
    Description: RNA editing is a fascinating phenomenon that is found in both animal and plant cells. By converting an adenosine base to an inosine (which behaves like guanosine) in RNA that has already been transcribed, certain RNA sequences (and hence the amino acids they encode) are altered. In a Perspective, Keegan, Gallo and O'Connell explore new results showing that activity of the editing enzyme ADAR1 is crucial for normal development of red blood cells in mouse embryos.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Keegan, L P -- Gallo, A -- O'Connell, M A -- New York, N.Y. -- Science. 2000 Dec 1;290(5497):1707-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, UK. liam.keegan@hgu.mrc.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11186391" target="_blank"〉PubMed〈/a〉
    Keywords: Adenosine/metabolism ; Adenosine Deaminase/chemistry/*genetics/*metabolism ; Animals ; Base Pairing ; Central Nervous System/metabolism ; Chimera ; Drosophila/genetics/metabolism ; Embryo, Mammalian/cytology ; Embryo, Nonmammalian ; *Erythropoiesis ; Gene Dosage ; Hematopoietic Stem Cells/cytology/enzymology ; Inosine/metabolism ; Liver/metabolism ; Mice ; Mutation ; Phenotype ; Protein Structure, Tertiary ; *RNA Editing ; RNA Precursors/metabolism ; RNA, Double-Stranded/metabolism ; RNA-Binding Proteins ; Receptors, AMPA/genetics ; Stem Cells/cytology/enzymology ; Teratoma/genetics/pathology
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-24
    Description: Once perceived to be at best ambivalent about science policy, President Bill Clinton is now credited with steering the U.S. government's $80-billion-plus R&D enterprise through one of its most perilous and productive decades. Along the way, supporters say, Clinton and his science-savvy vice president, Al Gore, have won respect from researchers. Although the reviews are not uniformly good, even critics agree that Clinton's term is ending on a much higher note for science than what many initially expected from the former Arkansas governor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2000 Dec 22;290(5500):2234-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11188713" target="_blank"〉PubMed〈/a〉
    Keywords: Budgets ; Government Agencies/economics ; National Institutes of Health (U.S.)/economics ; *Politics ; *Public Policy ; *Research ; Research Support as Topic ; *Science ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-07
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Normile, D -- Marshall, E -- New York, N.Y. -- Science. 2000 Mar 17;287(5460):1958-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10755951" target="_blank"〉PubMed〈/a〉
    Keywords: *Controlled Clinical Trials as Topic/standards ; *Drug Approval ; Europe ; Guidelines as Topic ; *International Cooperation ; Japan ; Placebos ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-24
    Description: A University of Texas (UT) immunologist has admitted to federal officials that he falsified research results over at least a 5-year period, leaving a trail of retracted papers and disgruntled collaborators. The scientist, who resigned and has been barred from receiving federal research grants, was found to have repeatedly duped colleagues by spiking test tubes with doses of a radioactive marker that produced positive results, according to detailed reports by UT and federal investigators.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2000 Oct 13;290(5490):245-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11183367" target="_blank"〉PubMed〈/a〉
    Keywords: *Allergy and Immunology ; Animals ; HLA-B27 Antigen/genetics ; Mice ; Research/*standards ; *Scientific Misconduct ; Texas ; United States ; United States Office of Research Integrity ; Universities
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-02-24
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2000 Oct 6;290(5489):23.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11183138" target="_blank"〉PubMed〈/a〉
    Keywords: Animal Testing Alternatives/*legislation & jurisprudence ; Animal Welfare/*legislation & jurisprudence ; Animals ; *Animals, Laboratory ; Birds ; Mice ; Rats ; United States ; United States Department of Agriculture/*legislation & jurisprudence
    Print ISSN: 0036-8075
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  • 10
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-05-02
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Parens, E -- Juengst, E -- New York, N.Y. -- Science. 2001 Apr 20;292(5516):397.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11330273" target="_blank"〉PubMed〈/a〉
    Keywords: *Bioethics ; Cytoplasm/*transplantation ; *DNA, Mitochondrial ; Female ; Financing, Government ; *Gene Transfer, Horizontal ; Genetic Research ; Guidelines as Topic ; Humans ; *Ovum ; Public Policy ; *Reproductive Techniques ; Research Support as Topic ; United States
    Print ISSN: 0036-8075
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  • 11
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-10
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gillooly, D J -- Stenmark, H -- New York, N.Y. -- Science. 2001 Feb 9;291(5506):993-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11232585" target="_blank"〉PubMed〈/a〉
    Keywords: Adaptor Proteins, Vesicular Transport ; Binding Sites ; Carrier Proteins/chemistry/*metabolism ; Cell Membrane/metabolism ; Clathrin/metabolism ; Coated Pits, Cell-Membrane/metabolism ; *Endocytosis ; Models, Biological ; Nerve Tissue Proteins/chemistry/*metabolism ; Neuropeptides/chemistry/*metabolism ; Phosphatidylinositol 4,5-Diphosphate/*metabolism ; Phosphoproteins/chemistry/*metabolism ; Protein Folding ; Protein Structure, Secondary ; Protein Structure, Tertiary ; *Vesicular Transport Proteins
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  • 12
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-05-02
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2001 Apr 27;292(5517):614-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11330302" target="_blank"〉PubMed〈/a〉
    Keywords: Anti-HIV Agents ; Developing Countries ; Drug Costs ; Drug Industry ; *Drugs, Essential/economics ; Humans ; *National Institutes of Health (U.S.) ; *Patents as Topic ; Research Support as Topic ; United States ; *Universities
    Print ISSN: 0036-8075
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  • 13
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-28
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2001 Mar 23;291(5512):2288.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11269282" target="_blank"〉PubMed〈/a〉
    Keywords: *Biological Warfare ; *International Cooperation ; Research ; United States
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 14
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-21
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2001 Mar 16;291(5511):2060.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11256387" target="_blank"〉PubMed〈/a〉
    Keywords: *Academic Medical Centers ; *Biomedical Research ; Clinical Trials as Topic/standards ; *Conflict of Interest ; Government Regulation ; Guidelines as Topic ; Humans ; Industry ; Lobbying ; Research/*standards ; United States ; *United States Dept. of Health and Human Services
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 15
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-06-16
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2001 Jun 15;292(5524):1989.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11408636" target="_blank"〉PubMed〈/a〉
    Keywords: Administrative Personnel/history ; History, 20th Century ; History, 21st Century ; National Institutes of Health (U.S.)/history/*organization & administration ; United States
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  • 16
    Publication Date: 2001-04-21
    Description: Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cramer, P -- Bushnell, D A -- Kornberg, R D -- GM49985/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Jun 8;292(5523):1863-76. Epub 2001 Apr 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305-5126, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11313498" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Binding Sites ; Conserved Sequence ; Crystallography, X-Ray ; DNA, Fungal/chemistry/metabolism ; Fourier Analysis ; Hydrogen Bonding ; Magnesium/metabolism ; Metals/metabolism ; Models, Molecular ; Molecular Sequence Data ; Promoter Regions, Genetic ; Protein Conformation ; Protein Structure, Quaternary ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Protein Subunits ; RNA Polymerase II/*chemistry/*metabolism ; RNA Processing, Post-Transcriptional ; RNA, Fungal/biosynthesis/chemistry/metabolism ; RNA, Messenger/biosynthesis/chemistry/metabolism ; Saccharomyces cerevisiae/*enzymology/genetics ; Transcription Factors/metabolism ; *Transcription, Genetic
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  • 17
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-08-11
    Description: Chromatin, the physiological template of all eukaryotic genetic information, is subject to a diverse array of posttranslational modifications that largely impinge on histone amino termini, thereby regulating access to the underlying DNA. Distinct histone amino-terminal modifications can generate synergistic or antagonistic interaction affinities for chromatin-associated proteins, which in turn dictate dynamic transitions between transcriptionally active or transcriptionally silent chromatin states. The combinatorial nature of histone amino-terminal modifications thus reveals a "histone code" that considerably extends the information potential of the genetic code. We propose that this epigenetic marking system represents a fundamental regulatory mechanism that has an impact on most, if not all, chromatin-templated processes, with far-reaching consequences for cell fate decisions and both normal and pathological development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jenuwein, T -- Allis, C D -- GM53512/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Aug 10;293(5532):1074-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Research Institute of Molecular Pathology (IMP) at the Vienna Biocenter, Dr. Bohrgasse 7, A-1030 Vienna, Austria. jenuwein@nt.imp.univie.ac.at〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11498575" target="_blank"〉PubMed〈/a〉
    Keywords: Acetylation ; Amino Acid Sequence ; Animals ; Chromatin/chemistry/metabolism/ultrastructure ; *Gene Expression Regulation ; *Gene Silencing ; Genomic Imprinting ; Histones/chemistry/genetics/*metabolism ; Methylation ; Molecular Sequence Data ; Phosphorylation ; Protein Structure, Tertiary ; Transcription, Genetic ; Transcriptional Activation
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  • 18
    Publication Date: 2001-06-12
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goldhaber, P -- Greenspan, J S -- Bowen, W H -- Genco, R J -- Barker, B -- Greene, J C -- Allukian, M Jr -- McCallum, C A -- Slavkin, H -- New York, N.Y. -- Science. 2001 Jun 8;292(5523):1835-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11398827" target="_blank"〉PubMed〈/a〉
    Keywords: Budgets ; National Institutes of Health (U.S.)/economics/legislation & ; jurisprudence/*organization & administration ; Research ; United States
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  • 19
    Publication Date: 2001-11-10
    Description: We describe a molecular switch based on the controlled methylation of nucleosome and the transcriptional cofactors, the CREB-binding proteins (CBP)/p300. The CBP/p300 methylation site is localized to an arginine residue that is essential for stabilizing the structure of the KIX domain, which mediates CREB recruitment. Methylation of KIX by coactivator-associated arginine methyltransferase 1 (CARM1) blocks CREB activation by disabling the interaction between KIX and the kinase inducible domain (KID) of CREB. Thus, CARM1 functions as a corepressor in cyclic adenosine monophosphate signaling pathway via its methyltransferase activity while acting as a coactivator for nuclear hormones. These results provide strong in vivo and in vitro evidence that histone methylation plays a key role in hormone-induced gene activation and define cofactor methylation as a new regulatory mechanism in hormone signaling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Xu, W -- Chen, H -- Du, K -- Asahara, H -- Tini, M -- Emerson, B M -- Montminy, M -- Evans, R M -- 9R01DK57978/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2001 Dec 21;294(5551):2507-11. Epub 2001 Nov 8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Gene Expression Laboratory, Department of Biological Chemistry, University of California Davis Cancer Center/Basic Science, Sacramento, CA 95817, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11701890" target="_blank"〉PubMed〈/a〉
    Keywords: Acetyltransferases/metabolism ; Amino Acid Sequence ; Animals ; Apoptosis ; Cell Line ; Cyclic AMP Response Element-Binding Protein/metabolism ; Dimerization ; E1A-Associated p300 Protein ; *Gene Expression Regulation ; Genes, Reporter ; Histone Acetyltransferases ; Histones/metabolism ; Methylation ; Molecular Sequence Data ; Nerve Growth Factor/pharmacology ; Nuclear Proteins/chemistry/*metabolism ; PC12 Cells ; Protein Structure, Tertiary ; Protein-Arginine N-Methyltransferases/*metabolism ; Rats ; Receptors, Retinoic Acid/*metabolism ; Recombinant Fusion Proteins/metabolism ; Retinoid X Receptors ; *Saccharomyces cerevisiae Proteins ; Signal Transduction ; Somatostatin/genetics ; Trans-Activators/chemistry/*metabolism ; Transcription Factors/metabolism ; *Transcription, Genetic ; Transcriptional Activation ; Transfection ; Tretinoin/metabolism/pharmacology
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-05-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lanza, R P -- Cibelli, J B -- West, M D -- Dorff, E -- Tauer, C -- Green, R M -- New York, N.Y. -- Science. 2001 May 18;292(5520):1299.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11360981" target="_blank"〉PubMed〈/a〉
    Keywords: *Bioethics ; Cell Differentiation ; Cell Line ; *Embryo Research ; Embryo, Mammalian/cytology ; Financing, Organized ; *Government Regulation ; Humans ; National Institutes of Health (U.S.) ; Regeneration ; Research/economics/*legislation & jurisprudence/standards ; *Stem Cells/cytology ; United States ; United States Dept. of Health and Human Services
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  • 21
    Publication Date: 2001-05-02
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goldin, D S -- Dahl, C A -- Olsen, K L -- Ostrach, L H -- Klausner, R D -- New York, N.Y. -- Science. 2001 Apr 20;292(5516):443-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Aeronautics and Space Administration (NASA), Washington, DC 20546, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11330296" target="_blank"〉PubMed〈/a〉
    Keywords: Biomarkers ; Biomarkers, Tumor ; *Biosensing Techniques ; Miniaturization ; *National Institutes of Health (U.S.) ; *Neoplasms/diagnosis/prevention & control/therapy ; *Research ; *Space Flight ; Spacecraft ; United States ; *United States National Aeronautics and Space Administration
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-07
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, E -- New York, N.Y. -- Science. 2001 Jan 26;291(5504):575.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11229397" target="_blank"〉PubMed〈/a〉
    Keywords: Clinical Trials as Topic ; *Confidentiality ; *Dna ; Databases, Factual ; Ethics, Medical ; *Genetic Research ; *Genetics, Medical ; Humans ; Informed Consent ; Internet ; *Medical Records Systems, Computerized ; Private Sector ; *Research ; United States
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  • 23
    Publication Date: 2001-03-17
    Description: Chloroplasts relocate their positions in a cell in response to the intensity of incident light, moving to the side wall of the cell to avoid strong light, but gathering at the front face under weak light to maximize light interception. Here, Arabidopsis thaliana mutants defective in the avoidance response were isolated, and the mutated gene was identified as NPL1 (NPH-like 1), a homolog of NPH1 (nonphototropic hypocotyl 1), a blue light receptor used in phototropism. Hence, NPL1 is likely a blue light receptor regulating the avoidance response under strong light.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kagawa, T -- Sakai, T -- Suetsugu, N -- Oikawa, K -- Ishiguro, S -- Kato, T -- Tabata, S -- Okada, K -- Wada, M -- New York, N.Y. -- Science. 2001 Mar 16;291(5511):2138-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉"Unit Process and Combined Circuit," PRESTO, Japan Science and Technology Corporation, 1-8, Honcho 4-chome, Kawaguchi-city, Saitama 332-0012, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11251116" target="_blank"〉PubMed〈/a〉
    Keywords: Alleles ; Arabidopsis/genetics/*physiology/ultrastructure ; *Arabidopsis Proteins ; Cell Membrane/metabolism ; Chloroplasts/*physiology ; Genes, Plant ; *Light ; Movement ; Mutation ; Phosphoproteins/chemistry/physiology ; Phototropism ; Plant Leaves/metabolism ; Plant Proteins/chemistry/*genetics/*physiology ; Plant Structures/metabolism ; Protein Structure, Tertiary ; RNA, Messenger/genetics/metabolism ; RNA, Plant/genetics/metabolism
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-24
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jungck, J R -- New York, N.Y. -- Science. 2001 Oct 19;294(5542):519-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11669093" target="_blank"〉PubMed〈/a〉
    Keywords: Academies and Institutes ; Government Agencies ; National Institutes of Health (U.S.) ; Science/*education ; United States ; *Universities
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  • 25
    Publication Date: 2001-06-26
    Description: Clinical studies with the Abl tyrosine kinase inhibitor STI-571 in chronic myeloid leukemia demonstrate that many patients with advanced stage disease respond initially but then relapse. Through biochemical and molecular analysis of clinical material, we find that drug resistance is associated with the reactivation of BCR-ABL signal transduction in all cases examined. In six of nine patients, resistance was associated with a single amino acid substitution in a threonine residue of the Abl kinase domain known to form a critical hydrogen bond with the drug. This substitution of threonine with isoleucine was sufficient to confer STI-571 resistance in a reconstitution experiment. In three patients, resistance was associated with progressive BCR-ABL gene amplification. These studies provide evidence that genetically complex cancers retain dependence on an initial oncogenic event and suggest a strategy for identifying inhibitors of STI-571 resistance.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gorre, M E -- Mohammed, M -- Ellwood, K -- Hsu, N -- Paquette, R -- Rao, P N -- Sawyers, C L -- GM07185/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Aug 3;293(5531):876-80. Epub 2001 Jun 21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medicine, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11423618" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Amino Acid Substitution ; Antineoplastic Agents/metabolism/pharmacology/therapeutic use ; Base Sequence ; Benzamides ; Blast Crisis/genetics ; Cell Line ; Drug Resistance, Neoplasm/genetics ; Fusion Proteins, bcr-abl/*metabolism ; Gene Amplification ; *Genes, abl ; Humans ; Hydrogen Bonding ; Imatinib Mesylate ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*drug therapy/*genetics ; Molecular Sequence Data ; Philadelphia Chromosome ; Phosphorylation ; Piperazines/metabolism/*pharmacology/therapeutic use ; Point Mutation ; Protein Structure, Tertiary ; Proto-Oncogene Proteins/metabolism ; Proto-Oncogene Proteins c-abl/antagonists & ; inhibitors/chemistry/*genetics/metabolism ; Proto-Oncogene Proteins c-crk ; Pyrimidines/metabolism/*pharmacology/therapeutic use ; Recurrence ; Signal Transduction
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-03
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bell, A C -- West, A G -- Felsenfeld, G -- New York, N.Y. -- Science. 2001 Jan 19;291(5503):447-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-0540, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11228144" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Chromatin/chemistry/*genetics ; Drosophila/genetics ; Enhancer Elements, Genetic ; *Gene Expression Regulation ; Gene Silencing ; *Genome ; Genomic Imprinting ; Humans ; Models, Genetic ; Promoter Regions, Genetic ; Protein Structure, Tertiary ; *Regulatory Sequences, Nucleic Acid ; Saccharomyces cerevisiae/genetics ; Vertebrates/genetics
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-04-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Taubes, G -- New York, N.Y. -- Science. 2001 Mar 30;291(5513):2538.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11286267" target="_blank"〉PubMed〈/a〉
    Keywords: *Diet, Fat-Restricted ; Dietary Fats/*administration & dosage ; Female ; Humans ; *Life Expectancy ; Male ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-08-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pennisi, E -- New York, N.Y. -- Science. 2001 Aug 10;293(5532):1065.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11498571" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Chromatin/physiology ; England ; Gene Expression Regulation ; History, 20th Century ; History, 21st Century ; Molecular Biology/history ; United States
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  • 29
    Publication Date: 2001-06-09
    Description: The p53 protein is present in low amounts in normally growing cells and is activated in response to physiological insults. MDM2 regulates p53 either through inhibiting p53's transactivating function in the nucleus or by targeting p53 degradation in the cytoplasm. We identified a previously unknown nuclear export signal (NES) in the amino terminus of p53, spanning residues 11 to 27 and containing two serine residues phosphorylated after DNA damage, which was required for p53 nuclear export in colloboration with the carboxyl-terminal NES. Serine-15-phosphorylated p53 induced by ultraviolet irradiation was not exported. Thus, DNA damage-induced phosphorylation may achieve optimal p53 activation by inhibiting both MDM2 binding to, and the nuclear export of, p53.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, Y -- Xiong, Y -- CA65572/CA/NCI NIH HHS/ -- K01 CA087580/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2001 Jun 8;292(5523):1910-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, NC 27599-7295, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11397945" target="_blank"〉PubMed〈/a〉
    Keywords: Active Transport, Cell Nucleus ; Amino Acid Sequence ; Animals ; Cell Fusion ; Cell Line ; Cell Nucleus/*metabolism ; Cells, Cultured ; Cytoplasm/metabolism ; *DNA Damage ; Mice ; Molecular Sequence Data ; Mutation ; *Nuclear Proteins ; Phosphorylation ; Phosphoserine/metabolism ; *Protein Sorting Signals ; Protein Structure, Tertiary ; Proteins/genetics/metabolism ; Proto-Oncogene Proteins/metabolism ; Proto-Oncogene Proteins c-mdm2 ; Recombinant Fusion Proteins/metabolism ; Transfection ; Tumor Suppressor Protein p14ARF ; Tumor Suppressor Protein p53/*chemistry/genetics/*metabolism ; Ubiquitins/metabolism ; Ultraviolet Rays
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-09-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 Sep 21;293(5538):2189.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11567118" target="_blank"〉PubMed〈/a〉
    Keywords: Arsenic/*adverse effects ; *Carcinogens ; Chile ; Humans ; Maximum Allowable Concentration ; National Academy of Sciences (U.S.) ; Neoplasms/*chemically induced/etiology ; Risk Assessment ; Taiwan ; United States ; United States Environmental Protection Agency ; Water Pollutants, Chemical/*adverse effects ; *Water Supply
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-09-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 Aug 31;293(5535):1575.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11533456" target="_blank"〉PubMed〈/a〉
    Keywords: Drug Costs ; Drug Design ; Financing, Government ; National Institutes of Health (U.S.)/*economics ; *Patents as Topic ; Pharmaceutical Preparations/*economics ; Research Support as Topic ; *Taxes ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-18
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 Dec 14;294(5550):2263-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11743168" target="_blank"〉PubMed〈/a〉
    Keywords: History, 21st Century ; Humans ; Medical Oncology/history ; National Institutes of Health (U.S.)/*organization & administration ; *Neoplasms ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-01
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 Nov 30;294(5548):1806-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11729275" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Conservation of Natural Resources/*legislation & jurisprudence ; *Ecology ; *Fisheries/legislation & jurisprudence ; Government ; *Salmon/physiology ; United States
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  • 34
    Publication Date: 2001-12-01
    Description: Heterotrimeric GTP-binding proteins (G proteins) control cellular functions by transducing signals from the outside to the inside of cells. Regulator of G protein signaling (RGS) proteins are key modulators of the amplitude and duration of G protein-mediated signaling through their ability to serve as guanosine triphosphatase-activating proteins (GAPs). We have identified RGS-PX1, a Galpha(s)-specific GAP. The RGS domain of RGS-PX1 specifically interacted with Galpha(s), accelerated its GTP hydrolysis, and attenuated Galpha(s)-mediated signaling. RGS-PX1 also contains a Phox (PX) domain that resembles those in sorting nexin (SNX) proteins. Expression of RGS-PX1 delayed lysosomal degradation of the EGF receptor. Because of its bifunctional role as both a GAP and a SNX, RGS-PX1 may link heterotrimeric G protein signaling and vesicular trafficking.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zheng, B -- Ma, Y C -- Ostrom, R S -- Lavoie, C -- Gill, G N -- Insel, P A -- Huang, X Y -- Farquhar, M G -- AG14563/AG/NIA NIH HHS/ -- CA58689/CA/NCI NIH HHS/ -- DK17780/DK/NIDDK NIH HHS/ -- GM56904/GM/NIGMS NIH HHS/ -- HL53773/HL/NHLBI NIH HHS/ -- HL63885/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2001 Nov 30;294(5548):1939-42.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093-0651, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11729322" target="_blank"〉PubMed〈/a〉
    Keywords: Adrenergic beta-2 Receptor Agonists ; Amino Acid Sequence ; Animals ; COS Cells ; Carrier Proteins/chemistry/*metabolism ; Cattle ; Cell Line ; Cyclic AMP/metabolism ; Endosomes/chemistry/metabolism ; GTP-Binding Protein alpha Subunits, Gs/antagonists & inhibitors/*metabolism ; GTPase-Activating Proteins/chemistry/*metabolism ; Guanosine Triphosphate/metabolism ; Humans ; Mitogen-Activated Protein Kinases/metabolism ; Molecular Sequence Data ; Protein Binding ; Protein Interaction Mapping ; Protein Structure, Tertiary ; Protein Transport ; RGS Proteins/chemistry/*metabolism ; Receptor, Epidermal Growth Factor/metabolism ; Receptors, Adrenergic, beta-2/genetics/metabolism ; Sequence Alignment ; Signal Transduction ; Sorting Nexins ; Substrate Specificity ; *Vesicular Transport Proteins
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lawler, A -- New York, N.Y. -- Science. 2001 Oct 26;294(5543):758-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11679636" target="_blank"〉PubMed〈/a〉
    Keywords: *Exobiology/history ; Extraterrestrial Environment ; History, 20th Century ; History, 21st Century ; *Space Flight/history ; United States ; United States National Aeronautics and Space Administration/history/*organization ; & administration
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-09-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lawler, A -- New York, N.Y. -- Science. 2001 Sep 7;293(5536):1747-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11546847" target="_blank"〉PubMed〈/a〉
    Keywords: Budgets/*legislation & jurisprudence ; *Government ; Public Policy ; Research/economics/legislation & jurisprudence ; Science/*economics/legislation & jurisprudence ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-06-30
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thacker, P -- New York, N.Y. -- Science. 2001 Jun 29;292(5526):2427.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11431551" target="_blank"〉PubMed〈/a〉
    Keywords: Animal Husbandry ; Animals ; *Animals, Wild ; *Cattle ; *Ecosystem ; Midwestern United States ; Poaceae ; *Sciuridae ; United States ; Wyoming
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-07-28
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉De La Cruz, E M -- Pollard, T D -- New York, N.Y. -- Science. 2001 Jul 27;293(5530):616-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11474090" target="_blank"〉PubMed〈/a〉
    Keywords: Actin Depolymerizing Factors ; Actins/*chemistry/*metabolism ; Adenosine Diphosphate/chemistry/*metabolism ; Adenosine Triphosphate/chemistry/metabolism ; Biopolymers/chemistry/metabolism ; *Contractile Proteins ; Crystallography, X-Ray ; Hydrolysis ; Microfilament Proteins/metabolism ; Phosphates/metabolism ; Profilins ; Protein Binding ; Protein Conformation ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Protein Subunits ; Rhodamines/metabolism ; Thymosin/metabolism
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-06-02
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Guenin, L M -- New York, N.Y. -- Science. 2001 Jun 1;292(5522):1659-60.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11387460" target="_blank"〉PubMed〈/a〉
    Keywords: *Bioethics ; Catholicism ; *Embryo Research ; *Embryo, Mammalian/cytology ; Financing, Government ; Humans ; *Morals ; National Institutes of Health (U.S.) ; Philosophy ; Public Policy ; Religion and Science ; *Research ; Research Support as Topic ; *Stem Cells ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-07-14
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pennisi, E -- New York, N.Y. -- Science. 2001 Jul 13;293(5528):194-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11452094" target="_blank"〉PubMed〈/a〉
    Keywords: Budgets ; Financing, Government ; *Museums ; Salaries and Fringe Benefits ; United States
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  • 41
    Publication Date: 2001-06-02
    Description: The GGAs are a multidomain protein family implicated in protein trafficking between the Golgi and endosomes. Here, the VHS domain of GGA2 was shown to bind to the acidic cluster-dileucine motif in the cytoplasmic tail of the cation-independent mannose 6-phosphate receptor (CI-MPR). Receptors with mutations in this motif were defective in lysosomal enzyme sorting. The hinge domain of GGA2 bound clathrin, suggesting that GGA2 could be a link between cargo molecules and clathrin-coated vesicle assembly. Thus, GGA2 binding to the CI-MPR is important for lysosomal enzyme targeting.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhu, Y -- Doray, B -- Poussu, A -- Lehto, V P -- Kornfeld, S -- R01 CA-08759/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2001 Jun 1;292(5522):1716-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Internal Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11387476" target="_blank"〉PubMed〈/a〉
    Keywords: Adaptor Proteins, Vesicular Transport ; Amino Acid Motifs ; Amino Acid Sequence ; Animals ; *Carrier Proteins ; Cations ; Clathrin/metabolism ; Dipeptides/chemistry/metabolism ; L Cells (Cell Line) ; Lysosomes/*enzymology ; Mice ; Molecular Sequence Data ; Mutation ; Protein Sorting Signals ; Protein Structure, Tertiary ; *Protein Transport ; Proteins/chemistry/genetics/*metabolism ; Rats ; Receptor, IGF Type 2/*chemistry/genetics/*metabolism ; Recombinant Fusion Proteins/chemistry/metabolism ; Solubility ; Transcription Factor AP-1/metabolism ; Transport Vesicles/metabolism ; Two-Hybrid System Techniques ; trans-Golgi Network/metabolism
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-07-21
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lawler, A -- New York, N.Y. -- Science. 2001 Jul 20;293(5529):408.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11463885" target="_blank"〉PubMed〈/a〉
    Keywords: *Biological Science Disciplines ; Budgets ; *Extraterrestrial Environment ; International Cooperation ; *Research ; Research Support as Topic ; Spacecraft/*economics ; United States ; United States National Aeronautics and Space Administration/*economics
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-06-16
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thornton, J M -- New York, N.Y. -- Science. 2001 Jun 15;292(5524):2095-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University College Department of Biochemistry and Molecular Biology, London WC1E 6BT, UK. thornton@biochem.ucl.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11408660" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; *Computational Biology ; Computer Simulation ; Databases, Factual ; Evolution, Molecular ; Genome ; *Models, Molecular ; Peptide Library ; *Protein Conformation ; Protein Structure, Tertiary ; Proteins/*chemistry/*physiology ; Proteome ; Sequence Homology, Amino Acid
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-04-18
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pennisi, E -- New York, N.Y. -- Science. 2001 Apr 13;292(5515):183-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11305292" target="_blank"〉PubMed〈/a〉
    Keywords: *Academies and Institutes/economics ; Animals ; *Animals, Zoo ; Budgets ; *Conservation of Natural Resources ; Financing, Government ; *Museums ; Research ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-05-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 May 18;292(5520):1275-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11360969" target="_blank"〉PubMed〈/a〉
    Keywords: *Academies and Institutes ; *Greenhouse Effect ; Humans ; International Cooperation ; Japan ; *Public Policy ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-04-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 Mar 30;291(5513):2533.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11286262" target="_blank"〉PubMed〈/a〉
    Keywords: *Arsenic/adverse effects/analysis ; Humans ; Maximum Allowable Concentration ; Politics ; Risk Factors ; Taiwan/epidemiology ; United States ; United States Environmental Protection Agency ; Urologic Neoplasms/chemically induced/epidemiology ; *Water Pollutants, Chemical/adverse effects/analysis ; Water Supply/*standards
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-17
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 Mar 2;291(5509):1679-80.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11253177" target="_blank"〉PubMed〈/a〉
    Keywords: *Biomedical Research ; *Ethics ; *Ethics, Research ; Federal Government ; *Government Regulation ; Inservice Training ; *Public Policy ; Research/*standards ; Scientific Misconduct ; United States ; *United States Office of Research Integrity ; United States Public Health Service
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-08-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bergmans, M -- New York, N.Y. -- Science. 2001 Aug 3;293(5531):801.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11496901" target="_blank"〉PubMed〈/a〉
    Keywords: *Animal Welfare ; Animals ; *Animals, Laboratory ; *Lobbying ; Research ; United States ; *United States Department of Agriculture
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-07
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaiser, J -- New York, N.Y. -- Science. 2001 Jan 26;291(5504):572-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11229395" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Clinical Trials as Topic ; Confidentiality ; Ethics, Medical ; *Genetic Therapy/adverse effects ; *Government Regulation ; Humans ; Information Services ; National Institutes of Health (U.S.) ; Public Opinion ; Transplantation, Heterologous ; Truth Disclosure ; United States ; *United States Food and Drug Administration
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  • 50
    Publication Date: 2001-06-30
    Description: The pollen extracellular matrix contains proteins mediating species specificity and components needed for efficient pollination. We identified all proteins 〉10 kilodaltons in the Arabidopsis pollen coating and showed that most of the corresponding genes reside in two genomic clusters. One cluster encodes six lipases, whereas the other contains six lipid-binding oleosin genes, including GRP17, a gene that promotes efficient pollination. Individual oleosins exhibit extensive divergence between ecotypes, but the entire cluster remains intact. Analysis of the syntenic region in Brassica oleracea revealed even greater divergence, but a similar clustering of the genes. Such allelic flexibility may promote speciation in plants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mayfield, J A -- Fiebig, A -- Johnstone, S E -- Preuss, D -- New York, N.Y. -- Science. 2001 Jun 29;292(5526):2482-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL 60637, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11431566" target="_blank"〉PubMed〈/a〉
    Keywords: Alleles ; Amino Acid Motifs ; Amino Acid Sequence ; Arabidopsis/chemistry/*genetics ; *Arabidopsis Proteins ; Brassica/chemistry/genetics ; Expressed Sequence Tags ; Genes, Plant ; Genetic Variation ; Genome, Plant ; Lipase/*chemistry/genetics ; Molecular Sequence Data ; *Multigene Family ; Phosphotransferases/chemistry/genetics ; Plant Proteins/*chemistry/genetics ; Pollen/*chemistry ; Protein Structure, Tertiary ; *Proteome ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-21
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lawler, A -- New York, N.Y. -- Science. 2001 Mar 16;291(5511):2073.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11256397" target="_blank"〉PubMed〈/a〉
    Keywords: California ; Exobiology ; Facility Design and Construction ; *Industry ; Information Science ; *Laboratories ; *Research ; Technology ; United States ; *United States National Aeronautics and Space Administration ; *Universities
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  • 52
    Publication Date: 2001-09-08
    Description: Bcl-2 family members bearing only the BH3 domain are essential inducers of apoptosis. We identified a BH3-only protein, Bmf, and show that its BH3 domain is required both for binding to prosurvival Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf is sequestered to myosin V motors by association with dynein light chain 2. Certain damage signals, such as loss of cell attachment (anoikis), unleash Bmf, allowing it to translocate and bind prosurvival Bcl-2 proteins. Thus, at least two mammalian BH3-only proteins, Bmf and Bim, function to sense intracellular damage by their localization to distinct cytoskeletal structures.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Puthalakath, H -- Villunger, A -- O'Reilly, L A -- Beaumont, J G -- Coultas, L -- Cheney, R E -- Huang, D C -- Strasser, A -- CA 80188/CA/NCI NIH HHS/ -- R29 DC003299/DC/NIDCD NIH HHS/ -- New York, N.Y. -- Science. 2001 Sep 7;293(5536):1829-32.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Walter and Eliza Hall Institute of Medical Research, Melbourne, P.O. Royal Melbourne Hospital, 3050 VIC, Australia.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11546872" target="_blank"〉PubMed〈/a〉
    Keywords: *Adaptor Proteins, Signal Transducing ; Amino Acid Sequence ; Animals ; *Anoikis ; Apoptosis Regulatory Proteins ; Calmodulin-Binding Proteins/*metabolism ; Carrier Proteins/*chemistry/genetics/*metabolism ; Cell Line ; Cytoskeleton/metabolism ; *Drosophila Proteins ; Dyneins ; Gene Expression Profiling ; Humans ; *Membrane Proteins ; Mice ; Molecular Motor Proteins/*metabolism ; Molecular Sequence Data ; Mutation ; Myeloid Cell Leukemia Sequence 1 Protein ; *Myosin Type V ; Neoplasm Proteins/genetics/metabolism ; Nerve Tissue Proteins/*metabolism ; Protein Binding ; Protein Structure, Tertiary ; Protein Transport ; *Proto-Oncogene Proteins ; Proto-Oncogene Proteins c-bcl-2/chemistry/genetics/metabolism ; RNA, Messenger/analysis/genetics ; Transfection ; Two-Hybrid System Techniques
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-08-31
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rabino, I -- New York, N.Y. -- Science. 2001 Aug 24;293(5534):1433-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11526938" target="_blank"〉PubMed〈/a〉
    Keywords: *Bioethics ; Data Collection ; Embryo, Mammalian/*cytology ; *Genetics, Medical ; Humans ; *Research ; Societies, Scientific ; *Stem Cells ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-01
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, M -- New York, N.Y. -- Science. 2001 Nov 30;294(5548):1811.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11729279" target="_blank"〉PubMed〈/a〉
    Keywords: Anthrax/etiology/*microbiology ; Bacillus anthracis/*classification/*genetics ; Bioterrorism/*legislation & jurisprudence ; DNA Fingerprinting/*standards ; Humans ; Reproducibility of Results ; United States
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    Publication Date: 2001-05-12
    Description: Epigenetic silenced alleles of the Arabidopsis SUPERMAN locus (the clark kent alleles) are associated with dense hypermethylation at noncanonical cytosines (CpXpG and asymmetric sites, where X = A, T, C, or G). A genetic screen for suppressors of a hypermethylated clark kent mutant identified nine loss-of-function alleles of CHROMOMETHYLASE3 (CMT3), a novel cytosine methyltransferase homolog. These cmt3 mutants display a wild-type morphology but exhibit decreased CpXpG methylation of the SUP gene and of other sequences throughout the genome. They also show reactivated expression of endogenous retrotransposon sequences. These results show that a non-CpG DNA methyltransferase is responsible for maintaining epigenetic gene silencing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lindroth, A M -- Cao, X -- Jackson, J P -- Zilberman, D -- McCallum, C M -- Henikoff, S -- Jacobsen, S E -- GM07104/GM/NIGMS NIH HHS/ -- GM07185/GM/NIGMS NIH HHS/ -- GM29009/GM/NIGMS NIH HHS/ -- GM60398/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Jun 15;292(5524):2077-80. Epub 2001 May 10.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11349138" target="_blank"〉PubMed〈/a〉
    Keywords: Alleles ; Amino Acid Sequence ; Arabidopsis/*genetics/metabolism ; *Arabidopsis Proteins ; Base Sequence ; Chromosome Mapping ; Cloning, Molecular ; CpG Islands ; Crosses, Genetic ; Cytosine/metabolism ; *DNA Methylation ; DNA-Cytosine Methylases/chemistry/*genetics/*metabolism ; Dinucleoside Phosphates/metabolism ; Gene Expression Regulation, Plant ; *Gene Silencing ; Genes, Plant ; Molecular Sequence Data ; Mutagenesis ; Oligonucleotides/*metabolism ; Phenotype ; Protein Structure, Tertiary ; Retroelements ; Transcription Factors/*genetics
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-01
    Description: There seem to be numerous pathways for exporting mRNAs from the nucleus to the cytoplasm. But working out which set of export adaptors and receptors transport individual mRNAs has been very difficult. In a Perspective, Moore and Rosbash discuss a new strategy using cell-penetrating peptide inhibitors for unraveling the routes of mRNA export in living cells (Gallouzi and Steitz).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Moore, M J -- Rosbash, M -- New York, N.Y. -- Science. 2001 Nov 30;294(5548):1841-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA. mmoore@brandeis.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11729289" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antennapedia Homeodomain Protein ; *Antigens, Surface ; Biological Transport/drug effects ; Cell Membrane Permeability ; Cell Nucleus/drug effects/*metabolism ; Cytoplasm/drug effects/*metabolism ; ELAV Proteins ; ELAV-Like Protein 1 ; Fatty Acids, Unsaturated/metabolism/pharmacology ; Gene Products, rev/chemistry/metabolism ; HIV/genetics ; Homeodomain Proteins/chemistry/metabolism ; Humans ; Karyopherins/*metabolism ; Neuropeptides/metabolism ; Nuclear Proteins/metabolism ; *Nucleocytoplasmic Transport Proteins ; Peptide Fragments/chemistry/metabolism/pharmacology ; Protein Binding/drug effects ; Protein Structure, Tertiary ; RNA, Messenger/genetics/*metabolism ; RNA-Binding Proteins/antagonists & inhibitors/chemistry/*metabolism ; *Receptors, Cytoplasmic and Nuclear ; Saccharomyces cerevisiae Proteins/metabolism ; *Transcription Factors ; rev Gene Products, Human Immunodeficiency Virus
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, M -- New York, N.Y. -- Science. 2001 Oct 26;294(5543):759-61.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11679638" target="_blank"〉PubMed〈/a〉
    Keywords: Anthrax/*drug therapy/microbiology ; Anti-Bacterial Agents/pharmacology/therapeutic use ; Anti-Infective Agents/pharmacology/*therapeutic use ; *Antigens, Bacterial ; Bacillus anthracis/*drug effects/genetics ; Bacterial Toxins/chemistry/metabolism ; *Bioterrorism ; Ciprofloxacin/pharmacology/*therapeutic use ; Drug Resistance, Bacterial ; Drug Utilization ; Humans ; Mutation ; Receptors, Cell Surface/metabolism ; Respiratory Tract Infections/*drug therapy/microbiology ; United States ; United States Food and Drug Administration
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-04-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vogel, G -- New York, N.Y. -- Science. 2001 Apr 6;292(5514):31.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11294201" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cloning, Organism/adverse effects/*legislation & jurisprudence ; Congenital Abnormalities/etiology ; DNA Methylation ; Government ; Humans ; Nuclear Transfer Techniques ; United States ; United States Food and Drug Administration
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-09-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Higgins, C F -- Linton, K J -- New York, N.Y. -- Science. 2001 Sep 7;293(5536):1782-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, DuCane Road, London W12 0NN, UK. christopher.higgins@csc.mrc.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11546861" target="_blank"〉PubMed〈/a〉
    Keywords: ATP-Binding Cassette Transporters/*chemistry/metabolism ; Adenosine Triphosphate/metabolism ; Bacterial Proteins/*chemistry/metabolism ; Cell Membrane/metabolism ; Crystallization ; Crystallography, X-Ray/methods ; Dimerization ; Escherichia coli/*chemistry ; Membrane Proteins/*chemistry/metabolism ; Models, Biological ; P-Glycoprotein/chemistry/metabolism ; Protein Structure, Secondary ; Protein Structure, Tertiary
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-05-16
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Singer, S F -- New York, N.Y. -- Science. 2001 May 11;292(5519):1063-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11352049" target="_blank"〉PubMed〈/a〉
    Keywords: Conservation of Energy Resources ; *Greenhouse Effect ; Ice ; Models, Biological ; National Academy of Sciences (U.S.) ; Oceans and Seas ; Risk Assessment ; United Nations ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-03
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Falkow, S -- Kennedy, D -- New York, N.Y. -- Science. 2001 Jan 19;291(5503):397.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11228121" target="_blank"〉PubMed〈/a〉
    Keywords: *Animal Husbandry ; Animals ; Anti-Bacterial Agents/*therapeutic use ; Bacterial Infections/microbiology/veterinary ; Centers for Disease Control and Prevention (U.S.) ; *Drug Resistance, Microbial ; Drug Utilization ; Humans ; United States ; United States Food and Drug Administration ; *Veterinary Drugs
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-09-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brown, K -- New York, N.Y. -- Science. 2001 Aug 31;293(5535):1617-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11533477" target="_blank"〉PubMed〈/a〉
    Keywords: *Computer-Assisted Instruction/economics ; Costs and Cost Analysis ; *Education, Distance/economics ; Faculty ; *Internet ; Science/*education ; United States ; *Universities
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  • 63
    Publication Date: 2001-03-27
    Description: Protein actions are usually discussed in terms of static structures, but function requires motion. We find a strong correlation between phosphorylation-driven activation of the signaling protein NtrC and microsecond time-scale backbone dynamics. Using nuclear magnetic resonance relaxation, we characterized the motions of NtrC in three functional states: unphosphorylated (inactive), phosphorylated (active), and a partially active mutant. These dynamics are indicative of exchange between inactive and active conformations. Both states are populated in unphosphorylated NtrC, and phosphorylation shifts the equilibrium toward the active species. These results support a dynamic population shift between two preexisting conformations as the underlying mechanism of activation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Volkman, B F -- Lipson, D -- Wemmer, D E -- Kern, D -- GM62117/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Mar 23;291(5512):2429-33.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Magnetic Resonance Facility at Madison (NMRFAM), Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11264542" target="_blank"〉PubMed〈/a〉
    Keywords: Allosteric Regulation ; *Bacterial Proteins ; Binding Sites ; DNA-Binding Proteins/*chemistry/genetics/*metabolism ; Models, Molecular ; Motion ; Mutation ; Nuclear Magnetic Resonance, Biomolecular ; PII Nitrogen Regulatory Proteins ; Phosphorylation ; *Protein Conformation ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Signal Transduction ; Time ; *Trans-Activators ; *Transcription Factors
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-01
    Description: Understanding how biochemical pathways are connected in the cell is one of the big challenges facing cell biologists. In a Perspective, von Zastrow and Mostov describe new work that identifies a protein called RGS-PX1 as the linchpin that connects signal transduction activated by G protein-coupled receptors with membrane trafficking events.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉von Zastrow, M -- Mostov, K -- New York, N.Y. -- Science. 2001 Nov 30;294(5548):1845-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Psychiatry, University of California, San Francisco, CA 94143, USA. zastrow@itsa.ucsf.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11729293" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Arrestins/metabolism ; Carrier Proteins/chemistry/*metabolism ; Databases, Genetic ; GTPase-Activating Proteins/chemistry/*metabolism ; Heterotrimeric GTP-Binding Proteins/chemistry/*metabolism ; Humans ; Protein Binding ; Protein Structure, Tertiary ; Protein Transport ; RGS Proteins/chemistry/*metabolism ; Receptor, Epidermal Growth Factor/metabolism ; Signal Transduction ; Sorting Nexins
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-05-02
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Carpenter, J -- New York, N.Y. -- Science. 2001 Apr 27;292(5517):637-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11330316" target="_blank"〉PubMed〈/a〉
    Keywords: Crops, Agricultural/*genetics ; *Ecosystem ; *Genetic Engineering ; *Pesticides/toxicity ; Plants, Genetically Modified ; United States
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  • 66
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-11-29
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Magnus, D -- Caplan, A -- New York, N.Y. -- Science. 2001 Nov 23;294(5547):1651.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11724062" target="_blank"〉PubMed〈/a〉
    Keywords: Bioethics ; Cloning, Organism/*legislation & jurisprudence/standards ; Ethical Review/*standards ; Humans ; *National Academy of Sciences (U.S.) ; Peer Review/*standards ; Public Policy ; Research Personnel/standards ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-11-10
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, O -- New York, N.Y. -- Science. 2001 Nov 9;294(5545):1298.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11701920" target="_blank"〉PubMed〈/a〉
    Keywords: *Antigens, Bacterial ; *Bacillus anthracis ; Bacterial Toxins/chemistry/*metabolism ; Crystallography, X-Ray ; Endocytosis ; Hydrogen-Ion Concentration ; Macrophages/metabolism/microbiology ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Phagocytosis ; Protein Conformation ; Protein Structure, Tertiary ; Receptors, Cell Surface/chemistry/*metabolism ; Receptors, Peptide/chemistry/*metabolism
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  • 68
    Publication Date: 2001-11-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Naylor, R L -- Williams, S L -- Strong, D R -- New York, N.Y. -- Science. 2001 Nov 23;294(5547):1655-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Environmental Science and Policy, Stanford University, Stanford, CA 94305-6055, USA. roz@stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11721035" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Aquaculture/*economics/*legislation & jurisprudence/methods ; Carps/physiology ; Catfishes/parasitology ; *Ecosystem ; Fish Diseases/economics/*parasitology/transmission ; Fisheries/economics/legislation & jurisprudence/methods ; Food Chain ; Food Industry/economics/legislation & jurisprudence/methods ; Host-Parasite Interactions ; International Cooperation ; Marine Biology ; Mollusca/physiology ; Ostreidae/parasitology/physiology ; Pest Control, Biological/economics/methods ; Risk ; Salmon/parasitology/physiology ; Seaweed/physiology ; United States
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  • 69
    Publication Date: 2001-05-19
    Description: Mannose 6-phosphate receptors (MPRs) deliver lysosomal hydrolases from the Golgi to endosomes and then return to the Golgi complex. TIP47 recognizes the cytoplasmic domains of MPRs and is required for endosome-to-Golgi transport. Here we show that TIP47 also bound directly to the Rab9 guanosine triphosphatase (GTPase) in its active, GTP-bound conformation. Moreover, Rab9 increased the affinity of TIP47 for its cargo. A functional Rab9 binding site was required for TIP47 stimulation of MPR transport in vivo. Thus, a cytosolic cargo selection device may be selectively recruited onto a specific organelle, and vesicle budding might be coupled to the presence of an active Rab GTPase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Carroll, K S -- Hanna, J -- Simon, I -- Krise, J -- Barbero, P -- Pfeffer, S R -- DK37332/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2001 May 18;292(5520):1373-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11359012" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Substitution/genetics ; Animals ; Binding Sites ; Cattle ; Cytoplasm/metabolism ; DNA-Binding Proteins/*metabolism ; Endosomes/metabolism ; Golgi Apparatus/metabolism ; Guanosine 5'-O-(3-Thiotriphosphate)/metabolism ; *Intracellular Signaling Peptides and Proteins ; *Pregnancy Proteins ; Protein Binding ; Protein Structure, Tertiary ; Protein Transport ; Receptor, IGF Type 2/chemistry/*metabolism ; Recombinant Fusion Proteins/metabolism ; Substrate Specificity ; Vesicular Transport Proteins ; rab GTP-Binding Proteins/genetics/*metabolism
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-05-31
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Snyder, C S -- New York, N.Y. -- Science. 2001 May 25;292(5521):1485-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11379622" target="_blank"〉PubMed〈/a〉
    Keywords: *Agriculture ; Conservation of Natural Resources ; *Fertilizers ; Nitrogen ; *Oxygen ; United States ; *Water Pollutants, Chemical
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-13
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Holden, C -- New York, N.Y. -- Science. 2001 Oct 12;294(5541):396-411.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11598304" target="_blank"〉PubMed〈/a〉
    Keywords: Academic Medical Centers ; *Biological Science Disciplines ; Career Choice ; Career Mobility ; Education, Graduate ; *Employment ; Faculty ; Female ; Humans ; *Job Satisfaction ; Male ; Private Sector ; Public Sector ; Research ; *Research Personnel/economics ; *Salaries and Fringe Benefits ; *Surveys and Questionnaires ; Teaching ; United States ; Universities ; Women, Working
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-08-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Holden, C -- New York, N.Y. -- Science. 2001 Aug 10;293(5532):1025.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11498550" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Line ; Cloning, Organism/*legislation & jurisprudence ; Embryo Research ; Embryo, Mammalian/*cytology ; Humans ; Politics ; *Research ; *Stem Cells ; United States
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  • 73
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-10
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ferber, D -- New York, N.Y. -- Science. 2001 Feb 9;291(5506):968-73.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11232575" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Conservation of Natural Resources ; *Ecosystem ; Eutrophication ; *Fertilizers ; Nitrogen ; *Oxygen ; Phytoplankton/physiology ; United States ; *Water Pollutants, Chemical ; Zooplankton/physiology
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  • 74
    Publication Date: 2001-10-27
    Description: ErbB-4 is a transmembrane receptor tyrosine kinase that regulates cell proliferation and differentiation. After binding of its ligand heregulin (HRG) or activation of protein kinase C (PKC) by 12-O-tetradecanoylphorbol-13-acetate (TPA), the ErbB-4 ectodomain is cleaved by a metalloprotease. We now report a subsequent cleavage by gamma-secretase that releases the ErbB-4 intracellular domain from the membrane and facilitates its translocation to the nucleus. gamma-Secretase cleavage was prevented by chemical inhibitors or a dominant negative presenilin. Inhibition of gamma-secretase also prevented growth inhibition by HRG. gamma-Secretase cleavage of ErbB-4 may represent another mechanism for receptor tyrosine kinase-mediated signaling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ni, C Y -- Murphy, M P -- Golde, T E -- Carpenter, G -- CA24071/CA/NCI NIH HHS/ -- CA68485/CA/NCI NIH HHS/ -- DK20593/DK/NIDDK NIH HHS/ -- NS39072/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2001 Dec 7;294(5549):2179-81. Epub 2001 Oct 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11679632" target="_blank"〉PubMed〈/a〉
    Keywords: Active Transport, Cell Nucleus ; Amino Acid Sequence ; Amyloid Precursor Protein Secretases ; Animals ; Aspartic Acid Endopeptidases ; COS Cells ; Carbamates/pharmacology ; Cell Division/drug effects ; Cell Line ; Cell Membrane/metabolism ; Cell Nucleus/*metabolism ; Cytoplasm/metabolism ; Dipeptides/pharmacology ; Endopeptidases/*metabolism ; Fatty Acids, Unsaturated/pharmacology ; Humans ; Membrane Proteins/genetics/metabolism ; Metalloendopeptidases/metabolism ; Mice ; Molecular Sequence Data ; Mutation ; Neuregulin-1/pharmacology ; Presenilin-1 ; Protease Inhibitors/pharmacology ; Protein Structure, Tertiary ; Receptor, Epidermal Growth Factor/chemistry/*metabolism ; Receptor, ErbB-4 ; Recombinant Fusion Proteins/metabolism ; Signal Transduction ; Tetradecanoylphorbol Acetate/pharmacology ; Transcriptional Activation ; Tumor Cells, Cultured
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  • 75
    Publication Date: 2001-02-27
    Description: Bag (Bcl2-associated athanogene) domains occur in a class of cofactors of the eukaryotic chaperone 70-kilodalton heat shock protein (Hsp70) family. Binding of the Bag domain to the Hsp70 adenosine triphosphatase (ATPase) domain promotes adenosine 5'-triphosphate-dependent release of substrate from Hsp70 in vitro. In a 1.9 angstrom crystal structure of a complex with the ATPase of the 70-kilodalton heat shock cognate protein (Hsc70), the Bag domain forms a three-helix bundle, inducing a conformational switch in the ATPase that is incompatible with nucleotide binding. The same switch is observed in the bacterial Hsp70 homolog DnaK upon binding of the structurally unrelated nucleotide exchange factor GrpE. Thus, functional convergence has allowed proteins with different architectures to trigger a conserved conformational shift in Hsp70 that leads to nucleotide exchange.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sondermann, H -- Scheufler, C -- Schneider, C -- Hohfeld, J -- Hartl, F U -- Moarefi, I -- New York, N.Y. -- Science. 2001 Feb 23;291(5508):1553-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cellular Biochemistry, Max-Planck-Institut fur Biochemie, D-82152 Martinsried, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11222862" target="_blank"〉PubMed〈/a〉
    Keywords: Adenosine Diphosphate/metabolism ; Adenosine Triphosphatases/*chemistry/*metabolism ; Adenosine Triphosphate/metabolism ; Amino Acid Sequence ; Animals ; Bacterial Proteins/chemistry/metabolism ; Carrier Proteins/*chemistry/*metabolism ; Cattle ; Crystallography, X-Ray ; DNA-Binding Proteins ; *Escherichia coli Proteins ; Evolution, Molecular ; HSC70 Heat-Shock Proteins ; HSP70 Heat-Shock Proteins/*chemistry/*metabolism ; Heat-Shock Proteins/chemistry/metabolism ; Humans ; Hydrolysis ; Models, Molecular ; Molecular Sequence Data ; Protein Conformation ; Protein Isoforms ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Transcription Factors
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-26
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Dec 21;294(5551):2460-1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11752553" target="_blank"〉PubMed〈/a〉
    Keywords: Biotechnology/*legislation & jurisprudence ; Great Britain ; Jurisprudence ; Patents as Topic/*legislation & jurisprudence ; *Protein Engineering ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-06-09
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Klug, A -- New York, N.Y. -- Science. 2001 Jun 8;292(5523):1844-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11397933" target="_blank"〉PubMed〈/a〉
    Keywords: Binding Sites ; Crystallization ; Crystallography, X-Ray ; DNA, Fungal/chemistry/metabolism ; Gene Expression Regulation, Fungal ; Promoter Regions, Genetic ; Protein Conformation ; Protein Folding ; Protein Structure, Quaternary ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Protein Subunits ; RNA Polymerase II/*chemistry/*metabolism ; RNA, Fungal/biosynthesis/chemistry/metabolism ; RNA, Messenger/biosynthesis/chemistry/metabolism ; Saccharomyces cerevisiae/*enzymology/genetics ; Transcription Factors/isolation & purification/metabolism ; *Transcription, Genetic
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-26
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Dec 21;294(5551):2449.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11752543" target="_blank"〉PubMed〈/a〉
    Keywords: Academies and Institutes/*organization & administration ; Biological Science Disciplines ; Government ; History, 21st Century ; National Academy of Sciences (U.S.)/*organization & administration ; National Institutes of Health (U.S.)/organization & administration ; Neoplasms ; *Terrorism ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-26
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Dec 21;294(5551):2443.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11752537" target="_blank"〉PubMed〈/a〉
    Keywords: Anthrax ; Bioterrorism ; DNA Fingerprinting ; Humans ; International Cooperation ; Publishing ; *Research ; Research Support as Topic ; Security Measures/legislation & jurisprudence ; *Terrorism ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-10
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roberts, L -- New York, N.Y. -- Science. 2001 Feb 16;291(5507):1182-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11233424" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Databases, Factual ; Financing, Government/history ; *Genome, Human ; History, 20th Century ; History, 21st Century ; Human Genome Project/economics/*history ; Humans ; Interpersonal Relations ; National Institutes of Health (U.S.)/history ; Patents as Topic/history ; Private Sector ; Public Sector ; Research Support as Topic/history ; Sequence Analysis, DNA/*history/instrumentation/methods ; United States
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-18
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Catterall, W A -- New York, N.Y. -- Science. 2001 Dec 14;294(5550):2306-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of Washington, Seattle, WA 98195, USA. wcatt@u.washington.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11743190" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Motifs ; Amino Acid Sequence ; Bacillus/*chemistry/metabolism ; Bacterial Proteins/antagonists & inhibitors/chemistry/*metabolism ; Calcium Channels/chemistry/metabolism ; Ion Channel Gating ; Ion Transport ; Membrane Potentials ; Potassium Channel Blockers ; Potassium Channels/chemistry/metabolism ; Protein Conformation ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Sodium/*metabolism ; Sodium Channel Blockers ; Sodium Channels/*chemistry/*metabolism ; Static Electricity
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-12
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Dec 7;294(5549):2069-70.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11739924" target="_blank"〉PubMed〈/a〉
    Keywords: Academies and Institutes ; Animals ; Bacteria/*genetics/pathogenicity ; Entamoeba histolytica/*genetics/pathogenicity ; Fungi/*genetics/pathogenicity ; Gene Expression Profiling ; *Genomics ; National Institutes of Health (U.S.) ; Oligonucleotide Array Sequence Analysis ; United States
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  • 83
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-11-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Nov 23;294(5547):1637.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11721028" target="_blank"〉PubMed〈/a〉
    Keywords: *Animal Experimentation ; Animal Rights/economics/legislation & jurisprudence ; Animal Welfare/economics/*legislation & jurisprudence ; Animals ; Birds ; Government ; *Government Regulation ; Housing, Animal/economics/legislation & jurisprudence ; Mice ; *Models, Animal ; Rats ; Research Design/legislation & jurisprudence ; *Rodentia ; United States ; United States Department of Agriculture/*legislation & jurisprudence
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  • 84
    Publication Date: 2001-03-28
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roberts, R J -- Varmus, H E -- Ashburner, M -- Brown, P O -- Eisen, M B -- Khosla, C -- Kirschner, M -- Nusse, R -- Scott, M -- Wold, B -- New York, N.Y. -- Science. 2001 Mar 23;291(5512):2318-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉New England Biolabs, Beverly, MA 01915, USA. roberts@neb.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11269300" target="_blank"〉PubMed〈/a〉
    Keywords: *Archives ; Information Management ; *Information Services ; Information Storage and Retrieval ; *Internet ; National Institutes of Health (U.S.) ; *Periodicals as Topic ; *Publishing ; United States
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  • 85
    Publication Date: 2001-07-28
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cech, T R -- Egan, L W -- Doyle, C -- Gallin, E -- Lichtman, M A -- Queenan, C J 3rd -- Sung, N -- New York, N.Y. -- Science. 2001 Jul 27;293(5530):573.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11474070" target="_blank"〉PubMed〈/a〉
    Keywords: Career Choice ; Costs and Cost Analysis ; Education, Medical/*economics ; National Institutes of Health (U.S.) ; Physicians/economics ; Research/*education ; Research Personnel/*economics ; *Training Support ; United States
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  • 86
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-11-17
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fesik, S W -- Shi, Y -- New York, N.Y. -- Science. 2001 Nov 16;294(5546):1477-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cancer Research, Global Pharmaceutical Research & Development, Abbott Laboratories, Abbott Park, IL 60064, USA. stephen.fesik@abbott.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11711663" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Motifs ; Animals ; *Apoptosis ; Binding Sites ; Carrier Proteins/*chemistry/*metabolism ; *Caspase Inhibitors ; Caspases/chemistry/*metabolism ; Crystallography, X-Ray ; Cysteine Proteinase Inhibitors/chemistry/metabolism ; Dimerization ; Humans ; Hydrogen Bonding ; Intracellular Signaling Peptides and Proteins ; Mitochondria/metabolism ; Mitochondrial Proteins/*chemistry/*metabolism ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Proteins/*chemistry/*metabolism ; X-Linked Inhibitor of Apoptosis Protein
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  • 87
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-11-10
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Nov 9;294(5545):1255-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11701895" target="_blank"〉PubMed〈/a〉
    Keywords: Access to Information/*legislation & jurisprudence ; Financing, Government ; Internet ; *National Institutes of Health (U.S.)/economics/legislation & jurisprudence ; *Peer Review, Research ; Privacy ; *Research Support as Topic ; United States
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 88
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-08-25
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Walters, L -- New York, N.Y. -- Science. 2001 Aug 24;293(5534):1401.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11520952" target="_blank"〉PubMed〈/a〉
    Keywords: *Bioethics ; *Embryo Research ; *Embryo, Mammalian/cytology ; Financing, Government ; *Government Regulation ; Great Britain ; Humans ; Internationality ; National Institutes of Health (U.S.) ; *Public Policy ; Research ; *Research Support as Topic ; *Stem Cells ; United States
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 89
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-12-26
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nishioka, K -- Reinberg, D -- New York, N.Y. -- Science. 2001 Dec 21;294(5551):2497-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11752565" target="_blank"〉PubMed〈/a〉
    Keywords: Acetyltransferases/metabolism ; *Gene Expression Regulation ; Histone Acetyltransferases ; Methylation ; Nuclear Proteins/*metabolism ; Phosphorylation ; Promoter Regions, Genetic ; Protein Structure, Tertiary ; Protein-Arginine N-Methyltransferases/*metabolism ; Receptors, Cytoplasmic and Nuclear/metabolism ; *Saccharomyces cerevisiae Proteins ; Signal Transduction ; Trans-Activators/*metabolism ; Transcription Factors/metabolism ; *Transcription, Genetic
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  • 90
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-11-03
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Nov 2;294(5544):970.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11691957" target="_blank"〉PubMed〈/a〉
    Keywords: Administrative Personnel ; History, 21st Century ; National Institutes of Health (U.S.)/organization & administration ; Science ; Societies, Scientific/*organization & administration ; United States
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  • 91
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-20
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Oct 19;294(5542):493.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11641471" target="_blank"〉PubMed〈/a〉
    Keywords: Academies and Institutes/economics/*organization & administration ; Computational Biology ; History, 21st Century ; National Institutes of Health (U.S.)/organization & administration ; Neoplasms ; Research Support as Topic ; United States
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  • 92
    Publication Date: 2001-08-04
    Description: One of the most dominant influences in the patterning of multicellular embryos is exerted by the Hedgehog (Hh) family of secreted signaling proteins. Here, we identify a segment polarity gene in Drosophila melanogaster, skinny hedgehog (ski), and show that its product is required in Hh-expressing cells for production of appropriate signaling activity in embryos and in the imaginal precursors of adult tissues. The ski gene encodes an apparent acyltransferase, and we provide genetic and biochemical evidence that Hh proteins from ski mutant cells retain carboxyl-terminal cholesterol modification but lack amino-terminal palmitate modification. Our results suggest that ski encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of Hh, and further demonstrate that this lipid modification is required for the embryonic and larval patterning activities of the Hh signal.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chamoun, Z -- Mann, R K -- Nellen, D -- von Kessler, D P -- Bellotto, M -- Beachy, P A -- Basler, K -- New York, N.Y. -- Science. 2001 Sep 14;293(5537):2080-4. Epub 2001 Aug 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut fur Molekularbiologie and Zoologisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11486055" target="_blank"〉PubMed〈/a〉
    Keywords: Acylation ; Acyltransferases/chemistry/*genetics/*metabolism ; Amino Acid Sequence ; Amino Acid Substitution ; Animals ; Body Patterning ; Cholesterol/metabolism ; *Drosophila Proteins ; Drosophila melanogaster/embryology/*genetics/growth & development/metabolism ; Gene Expression ; Genes, Insect ; Hedgehog Proteins ; Insect Proteins/chemistry/genetics/*metabolism ; Molecular Sequence Data ; Mutation ; Palmitic Acid/*metabolism ; Protein Structure, Tertiary ; *Signal Transduction ; Transgenes
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  • 93
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-03-10
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Holden, C -- New York, N.Y. -- Science. 2001 Feb 9;291(5506):967.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11232574" target="_blank"〉PubMed〈/a〉
    Keywords: Amygdala/physiology ; Brain/*physiology ; *Deception ; Fear ; Government Agencies ; Humans ; Lasers ; *Lie Detection ; Magnetic Resonance Imaging ; Reproducibility of Results ; Research Support as Topic ; Thermography ; United States ; Voice
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  • 94
    Publication Date: 2001-03-21
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Robertson, G P -- Barry, P J -- Busta, F F -- Collier, R J -- Keen, N T -- Sederoff, R R -- Simpkins, W W -- Urban, T N -- New York, N.Y. -- Science. 2001 Mar 16;291(5511):2089-90.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11256402" target="_blank"〉PubMed〈/a〉
    Keywords: *Agriculture ; Research ; *Research Support as Topic ; United States ; United States Department of Agriculture/*economics
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  • 95
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-13
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chander, R -- Mervis, J -- New York, N.Y. -- Science. 2001 Oct 12;294(5541):395.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11598303" target="_blank"〉PubMed〈/a〉
    Keywords: *Biological Science Disciplines/economics ; *Salaries and Fringe Benefits ; Societies, Scientific ; *Surveys and Questionnaires ; United States
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  • 96
    Publication Date: 2001-11-27
    Description: We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are folded like actin, with distinctive surface features. Subunits ARPC2 p34 and ARPC4 p20 in the core of the complex associate through long carboxyl-terminal alpha helices and have similarly folded amino-terminal alpha/beta domains. ARPC1 p40 is a seven-blade beta propeller with an insertion that may associate with the side of an actin filament. ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Robinson, R C -- Turbedsky, K -- Kaiser, D A -- Marchand, J B -- Higgs, H N -- Choe, S -- Pollard, T D -- GM-26132/GM/NIGMS NIH HHS/ -- GM-26338/GM/NIGMS NIH HHS/ -- GM-56653/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Nov 23;294(5547):1679-84.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11721045" target="_blank"〉PubMed〈/a〉
    Keywords: Actin Cytoskeleton/*chemistry/*metabolism ; Actin-Related Protein 2 ; Actin-Related Protein 3 ; Actins/*chemistry/*metabolism ; Adenosine Triphosphate/metabolism ; Animals ; Cattle ; Crystallography, X-Ray ; *Cytoskeletal Proteins ; Macromolecular Substances ; Models, Biological ; Models, Molecular ; Muscle, Skeletal ; Protein Structure, Quaternary ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Protein Subunits ; Static Electricity ; Thymus Gland
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  • 97
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-13
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Malakoff, D -- New York, N.Y. -- Science. 2001 Oct 12;294(5541):279-81.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11598273" target="_blank"〉PubMed〈/a〉
    Keywords: Bioterrorism ; *Budgets ; Centers for Disease Control and Prevention (U.S.)/economics ; Embryo, Mammalian/cytology ; Humans ; National Institutes of Health (U.S.)/*economics ; *Research Support as Topic ; Stem Cells ; United States ; United States Dept. of Health and Human Services/economics
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  • 98
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2001-10-13
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Koenig, R -- New York, N.Y. -- Science. 2001 Oct 12;294(5541):284-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11598277" target="_blank"〉PubMed〈/a〉
    Keywords: Agriculture ; *Disasters ; Maryland ; *Plants ; Plants, Genetically Modified ; *Research ; United States ; *United States Department of Agriculture
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  • 99
    Publication Date: 2001-08-18
    Description: Arabidopsis seedling photomorphogenesis involves two antagonistically acting components, COP1 and HY5. COP1 specifically targets HY5 for degradation via the 26S proteasome in the dark through their direct physical interaction. Little is known regarding how light signals perceived by photoreceptors are transduced to regulate COP1. Arabidopsis has two related cryptochromes (cry1 and cry2) mediating various blue/ultraviolet-A light responses. Here we show that both photoactivated cryptochromes repress COP1 activity through a direct protein-protein contact and that this direct regulation is primarily responsible for the cryptochrome-mediated blue light regulation of seedling photomorphogenic development and genome expression profile.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, H -- Ma, L G -- Li, J M -- Zhao, H Y -- Deng, X W -- GM-47850/GM/NIGMS NIH HHS/ -- GM59507/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Oct 5;294(5540):154-8. Epub 2001 Aug 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11509693" target="_blank"〉PubMed〈/a〉
    Keywords: Arabidopsis/genetics/*growth & development/*metabolism ; *Arabidopsis Proteins ; Basic-Leucine Zipper Transcription Factors ; Carrier Proteins/chemistry/genetics/*metabolism ; Cell Nucleus/metabolism ; Crosses, Genetic ; Cryptochromes ; Darkness ; *Drosophila Proteins ; Expressed Sequence Tags ; *Eye Proteins ; Flavoproteins/genetics/*metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genes, Plant ; *Light ; Morphogenesis ; Mutation ; Nuclear Proteins/metabolism ; Oxidation-Reduction ; Phenotype ; *Photoreceptor Cells, Invertebrate ; Plant Proteins/chemistry/genetics/*metabolism ; Plants, Genetically Modified ; Precipitin Tests ; Protein Binding ; Protein Structure, Tertiary ; Receptors, G-Protein-Coupled ; Recombinant Fusion Proteins/metabolism ; Signal Transduction ; *Ubiquitin-Protein Ligases
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  • 100
    Publication Date: 2001-09-08
    Description: Multidrug resistance (MDR) is a serious medical problem and presents a major challenge to the treatment of disease and the development of novel therapeutics. ABC transporters that are associated with multidrug resistance (MDR-ABC transporters) translocate hydrophobic drugs and lipids from the inner to the outer leaflet of the cell membrane. To better elucidate the structural basis for the "flip-flop" mechanism of substrate movement across the lipid bilayer, we have determined the structure of the lipid flippase MsbA from Escherichia coli by x-ray crystallography to a resolution of 4.5 angstroms. MsbA is organized as a homodimer with each subunit containing six transmembrane alpha-helices and a nucleotide-binding domain. The asymmetric distribution of charged residues lining a central chamber suggests a general mechanism for the translocation of substrate by MsbA and other MDR-ABC transporters. The structure of MsbA can serve as a model for the MDR-ABC transporters that confer multidrug resistance to cancer cells and infectious microorganisms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chang, G -- Roth, C B -- GM61905-01/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Sep 7;293(5536):1793-800.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, MB-9, The Scripps Research Institute, La Jolla, CA 92037, USA. gchang@scripps.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11546864" target="_blank"〉PubMed〈/a〉
    Keywords: *ATP-Binding Cassette Transporters ; Adenosine Triphosphate/metabolism ; Amino Acid Sequence ; Bacterial Proteins/*chemistry/genetics/metabolism ; Binding Sites ; Biological Transport ; Crystallography, X-Ray ; Dimerization ; *Drug Resistance, Microbial ; *Drug Resistance, Multiple ; Escherichia coli/*enzymology ; Lipid A/metabolism ; Membrane Proteins/*chemistry/genetics/metabolism ; Models, Molecular ; Molecular Sequence Data ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Sequence Alignment ; Static Electricity ; Structure-Activity Relationship
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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