ALBERT

Notes: The role of tree plantations as facilitators of tropical forest restoration in degraded lands has been explored recently, but there are few data on the effect of different tree species on invasion of the plant understory. We evaluated early patterns of understory composition in three-year-old native tree plantations in lowland Costa Rica using two pure-species treatment (Jacaranda copaia and Vochysia guatemalensis) and one mixed-species treatment (J. copaia, V. guatemalensis, Stryphnodendron microstachyum, and Calophyllum brasiliense). We also monitored woody invasion in unplanted control areas dominated by grasses. The understory of the different plantation treatments differed in light environment, woody-plant growth and recruitment, and quantity and quality of woody regeneration. Forest tree invasion appeared to be enhanced under Vochysia, while shrubs were more abundant under the Jacaranda and mixed-species treatments. Woody plant growth, herbaceous cover, and understory light availability were highest under Jacaranda, intermediate under mixed species, and lowest under Vochysia. Soil-stored seeds seemed an important source for woody plant recruitment in Jacaranda and mixed species and of minimal importance under Vochysia, probably due to light suppression. It appears that competition from grasses is a major factor influencing early woody invasion in our study area. We found no woody recruitment after one year in the unplanted controls. We suggest that to promote the use of plantations as tools of forest restoration, there is a need to gather basic ecological information on how different tree species may influence patterns of plant understory colonization.

Notes: Prescribed burns are increasingly being used in ecological restoration and vegetation management. Despite the accumulation of scientific information on fire behavior and fire effects, however, in many cases fires are prescribed without consideration of such information and often simply because of evidence of past fires. Rather than basing fire management plans on ideas of the historical “natural” occurrence of fire, we present the case for fire management being based on the fire effects desired. Effective fire management and development of proper fire prescriptions require an understanding of fire processes and heat transfer that explain fire behavior characteristics, as well as an understanding of how fire behavior is coupled to specific fire effects. We provide a basic introduction to these concepts and processes, which will help in understanding the importance of having a more technical understanding of fire. The discussion includes the processes of heat transfer and the relative role of various fuel variables in these processes, as well as the concepts of fire intensity, rate of spread, fuel consumption, duff consumption, fire frequency, and the ecological effects associated with variation in these characteristics of fire behavior.

Notes: Restoration ecologists are increasingly aware of the potential to re-create chalk grassland on abandoned farmland. Success is often hampered by lack of desirable species in the seed bank and by poor dispersal from nearby sites. In certain schemes, the input of seed may be essential. Locally collected seed is desirable but availability is limited. We examined whether lower sowing rates than currently recommended may be successfully utilized, facilitating more-efficient use of available seed. Experimental plots on former agricultural land were sown at different rates in a randomized complete block, and the vegetation was surveyed for two years. We compared species richness and cover for chalk grassland plants and weeds - species not associated with chalk grassland communities. Values for cover and abundance were matched with data for communities of the British National Vegetation Classification (NVC). Species richness for chalk grassland plants increased with sowing rate and with time, although after two years there was no significant difference between the treatments sown at 0.4, 1.0, and 4.0 grams of seed per square meter. Weed species decreased with increasing rate and time. After two seasons, the vegetation on all treatment plots was similar to that of recognized NVC chalk grassland communities, while the controls were dominated by weeds and showed signs of developing into species-poor grassland. Higher rates rapidly eliminated weeds, but even a small inoculum of seed seemed to significantly enhance establishment of desirable plants and to reduce weed cover. We conclude that lower sowing rates would enable the desired vegetation to become established successfully, under appropriate conditions and management regimes. Lower rates allow for the re-creation of sizable areas using local seed, and they minimize damage to donor sites.

Notes: Books reviewed in this article: Ecology of Greenways — Design and Function of Linear Conservation Areas. Daniel S. Smith and Paul Cawood Hellmund, editors Reconstruction of Fragmented Ecosystems: Global and Regional Perspectives. D. Saunders, R. Hobbs, and P. Ehrlich, editors Defining Sustainable Forests G. H. Aplet, N. Johnson, J. T. Olson, and V. A. Sample, editors

Notes: Seed bank experiments are described to assess the species richness potential of coal slurry ponds reclaimed as wetlands (ranging from 6 to more than 40 years old). Experimental treatments test the drawdown and flooded conditions characteristic of the vegetation dynamics of emergent wetlands in the Upper Mississippi Valley. More seedlings, primarily annuals, emerged from exposed wet sediments (freely drained) than under continuous flooded sediments in cold ponds (339 versus 136 seedlings m−2, respectively) and in natural ponds (163 versus 47, respectively). More seeds were produced by plants established in freely drained conditions than under flooded conditions from sediments in the coal ponds (26546 versus 1842 seeds m−2, respectively) and the natural ponds (28430 versus 4526, respectively). Similarly, more biomass was also produced by these plants in freely drained than under flooded conditions in coal ponds (118 versus 47 g m−2, respectively) and natural ponds (118 versus 52, respectively). Fertilization (NPK) did not affect germination for the most part, but it did affect seed set and biomass production, especially for C4 annuals such as Echinochloa crusgalli and Panicum dichotomiflorum. I propose that lime (calcium carbonate) and fertilizer be applied during the first few scheduled drawdowns for these coal slurry ponds reclaimed as wetlands to increase the number of species and to allow their more rapid development as self-sustaining systems.

Notes: Efforts to restore fish communities of the Kissimmee River will require carefully defined criteria for assessing success. A goal of regaining communities mirroring those in the historical river may not be an appropriate target because the ecological conditions of the river before channelization are poorly known. The Kissimmee River is in a biogeographic region historically low in fish diversity, and no comparable rivers in that region remain substantially unaltered by human activity to permit their use as reference sites indicative of conditions in the Kissimmee before channelization. I propose alternative criteria for assessing restoration success emphasizing expectations for ecosystem function in similar floodplain rivers. Assessing ecosystem function will be less simple than assessing criteria such as fish condition or density of selected species. But criteria based solely on fish-population characteristics cannot be justified quantitatively. Information integrated from several levels of biotic organization (individuals, populations, communities, and systems) should be drawn upon in making conclusions about restoration success. I develop a conceptual model to outline aspects of ecosystem function that could serve as a basis for evaluation of the restoration of fish communities of the Kissimmee River. The model focuses on the dynamics of the flux of floodplain-channel nutrients and the movement of larvae, juvenile, and adult fishes and macroinvertebrates. The present community may be dominated more by species tolerant of low-oxygen conditions, such as gar and bowfin, than the restored community will be. I propose that nest sites may be the limiting recruitment success of substrate spawning species in the channelized river and that these species, including sunfish and large-mouth bass, will increase in abundance after restoration. Also, species relying on floodplain habitats, including sun-fish species, darters, and some minnows, may also increase in frequency with restoration of floodplain-channel hydro-logical conditions and habitats. The observation that no species are known to have disappeared from the Kissimmee River, and its relatively simple community structure compared to rivers of comparable size elsewhere, are encouraging for prospects of successful restoration.

Notes: Restoration of the Kissimmee River and floodplain ultimately will involve restoring 70 km of river channel and riparian zone and 11,000 ha of wetland over a period of two decades. Restoring ecosystem integrity is a crucial goal of the project, and the evaluation program is designed to assess the success of this endeavor. Major components of the riverine and floodplain ecosystem will be evaluated, guided by conceptual models of their structure and function. These studies will be referenced to historic conditions of the past and to present-day conditions in the channelized system. Enhanced connectivity and interactions between the river and floodplain, the interplay of abiotic and biotic variables, and interactions between trophic levels will restructure the channelized river and the largely drained floodplain that now exist. The key to evaluating the success of this ambitious project will be selecting measurements of the structure and function of the river and floodplain ecosystems that are responsive to this large-scale manipulation. The timing and duration of floodplain inundation, improved dissolved oxygen conditions, germination and establishment of wetland vegetation, and enhancement and expansion of rheophilic benthic invertebrate populations are critical initial elements of restoration. Further expected outcomes are an increase in the primary productivity of the ecosystem, expansion of the fish community into the reopened channels and onto the reflooded floodplain, and improved visitation and use by waterbirds in the restored regions. We highlight predictions of some of these key linkages and primary structural and functional attributes of the restored river and floodplain that should be measured.

Notes: Beach nourishment is an engineering solution to erosion of beaches. As in any restoration project, the goals of beach nourishment are the restoration of habitat to promote survival of plants and animals and to maintain aesthetically pleasing sites for humans. Unfortunately, beach nourishment sometimes alters parameters of the natural beach, decreasing the reproductive success of sea turtles. Engineers have recognized this problem and are working to improve nourishment practices. Biologists must specify problems incurred by sea turtles as a result of beach nourishment so that they may be addressed. A review of the literature on sea turtles and beach nourishment found certain problems repeatedly identified. For nesting females, characteristics induced by nourishment can cause (1) beach compaction, which can decrease nesting success, alter nest-chamber geometry, and alter nest concealment, and (2) escarpments, which can block turtles from reaching nesting areas. For eggs and hatchlings, nourishment can decrease survivorship and affect development by altering beach characteristics such as sand compaction, gaseous environment, hydric environment, contaminant levels, nutrient availability, and thermal environment. Also, nests can be covered with excess sand if nourishment is implemented in areas with incubating eggs. The extent and implication of each problem are discussed, and future research initiatives are proposed.

Notes: We investigated the role of artificially introduced Panicum virgatum (switch grass) on the sequential natural revegetation of 15- and 35-year-old tailings in the Adirondack region of northern New York. Switch grass covered approximately 48% of the 15-year-old Chaumont Tailings. Establishment of switch-grass stands improved the fertility of the site by adding organic matter, raising pH, and elevating cation exchange capacity and concentrations of major nutrients (N, P, and K). Switch-grass stands also aided the initial recruitment of such pioneer species as Populus spp. (aspens), Salix spp. (willows), and Betula spp. (birches). This facilitation of recruitment of woody species is explained as follows: (1) robust switch-grass stands physically captured the wind-disseminated seeds of these species; (2) switch grass acted as a “nurse crop” for these species, thus these species were able to increase their density vigorously through root or stem sprouting; (3) a combination of both. Switch grass decreased its cover (14%), however, as observed in the 35-year-old South Tailings. As switch grass declined, such vigorous “root-suckering” species as aspens increased in dominance, followed by the invasion of Prunus pensylvanica (pin cherry).

Notes: The purpose of this study was to clarify the effect of grazing exclosures on the recovery and rehabilitation of overgrazed steppe vegetation on varying slope aspects in the Loess Plateau of northwest China. The annual precipitation in the area studied was 400–480 mm. Soil samples were taken on nine slopes in the five-year exclosure and on five slopes outside the exclosure after a vegetation survey; they were then analyzed chemically. Mean number of species recorded per 0.25 m2 was lower on the south-facing slope than all other slopes. The reverse trend was observed for aerial biomass. Species diversity estimated by information content was higher in the grazing zone than in a 3200-ha protected zone within an exclosure. From species ordination by principal component analysis, species with lower coverage in the grazing zone were Poa sphondylodes, Roegneria purpurascens, Hierochloe odorata, and Potentilla bifurca, which are all recognized as indicator species for rehabilitation efforts. In the soil surface layer, calcium contents were low, and the total contents of carbon and nitrogen were high on the north-facing slope in the exclosure. The protection by exclosure of overgrazed steppe was seen to be effective because the accumulation of soil organic matter increased and water balance improved.

Notes: Theoretical work on population viability and extinction probabilities, empirical data from Canis lupus (gray wolf) populations, and expert opinion provide only general and conflicting conclusions about the number of wolves and the size of areas needed for conservation of wolf populations. There is no threshold population size or proven reserve design that guarantees long-term (century or more) survival for a gray wolf population. Most theoretical analyses of population viability have assumed a single, isolated population and lack of management intervention, neither of which is likely for wolves. Data on survival of actual wolf populations suggest greater resiliency than is indicated by theory. In our view, the previous theoretical treatments of population viability have not been appropriate to wolves, have contributed little to their conservation, and have created unnecessary dilemmas for wolf recovery programs by overstating the required population size. Nonetheless, viability as commonly understood may be problematic for small populations at the fringe of or outside the contiguous species range, unless they are part of a metapopulation. The capability of existing nature reserves to support viable wolf populations appears related to a variety of in situ circumstances, including size, shape and topography of the reserve; productivity, numbers, dispersion, and seasonal movement of prey; extent of poaching inside; degree of persecution outside; exposure to enzootica; attitudes of local people; and proximity to other wolf populations. We estimate that a population of 100 or more wolves and a reserve of several thousand square kilometers may be necessary to maintain a viable population in complete isolation, although 3000 km2 or even 500–1000 km2 may be adequate under favorable circumstances. In most cases, management intervention is probably necessary to assure the viability of relatively small, isolated populations. Because most reserves may be inadequate by themselves to ensure the long-term survival of wolf populations, favorable human attitudes toward the species and its management must be recognized as paramount, and cooperation of neighboring management jurisdictions will be increasingly important.

Notes: Dieout of Ammophila breviligulata— death of the major dune-stabilizing plants — has been observed along the north and mid-Atlantic coast of the United States for the past decade and a half. Pathogenic nematodes have been identified as the probable causal agents; they can bring about a complete dieout of sand dune vegetation, with Ammophila breviligulata being the first species to die. Typically, such an area would remain barren for up to five years before plants could be successfully introduced. Applications of fertilizer and dolomitic limestone were tested in the field as a possible management strategy to alleviate the vulnerability of a denuded dune to erosion by making it possible to plant such a site earlier than usual. These applications were also tested in an area of weakened and dying plants to determine if the vegetation could be saved before complete dieout occurred. By creating soil conditions conducive to vigorous plant growth, it was hypothesized that the plants could better withstand the stress of nematode attack. The addition of N-P-K macronutrient fertilizer resulted in increased growth and spread of plants introduced into a site where the grass had been dead for only one to two years. Results indicate that application of fertilizer would be necessary only every other year at most. Micronutrient application, at the concentration used in this study, had little or a somewhat detrimental effect. The addition of dolomitic limestone increased the survival of newly introduced plants. It was also found that the application of macronutrients to a site of moribund vegetation could not only rescue the plants in that site, but could also increase their growth, vigor, and spread, thereby preventing further loss of plant cover essential to dune stabilization.

Notes: Our objectives were to evaluate the use of microcatchments in the establishment of Leucaena retusa (little-leaf leadtree) and Atriplex canescens (four-wing saltbush) and their role in the initiation of autogenic landscape restoration processes on a shallow semiarid site. Three six-month-old seedlings of either Leucaena retusa or Atriplex canescens were planted in 1.5-m2 microcatchments. An equal number of seedlings was planted in control plots (unmodified soil surface). The water collection effects on shrub survival, standing biomass, and the natural immigration of herbaceous vegetation were determined over 42 months. Planting in microcatchment basins doubled Leucaena seedling survival and resulted in a five-fold increase in standing biomass, compared to the control, during the first growing season. There was a significant increase in soil organic matter in the microcatchment basins within 32 months. At the same time, microcatchments planted with Atriplex canescens seedlings had a ten-fold increase in seedling standing biomass compared to the control. Forty-two months after transplanting, the herbaceous standing crop was significantly greater near Atriplex canescens or in microcatchment basins than in plots with unmodified surface soil. Basins containing Atriplex produced significantly more herbaceous vegetation than basins containing Leucaena, and empty basins produced the least herbaceous vegetation of three basin treatments. These data suggest that landscape-scale procedures that concentrate scarce resources (water, organic matter, nutrients, and propagules), establish keystone species, and ameliorate microenvironmental conditions can initiate autogenic restoration of degraded semiarid ecosystems.

Notes: This paper interprets private label marketing as a retailer instrument for overcoming the double-marginalization problem inherent in the distribution of well-known manufacturer brands. Retailers with some degree of market power carry private label substitutes for popular national brands in order to capture more profit from the vertical structures they share with brand manufacturers. The net effect of private label marketing is to improve the performance of distribution channels. After presenting a formal model and deriving analytical results, the paper gathers some empirical evidence that supports these results.

Notes: This paper studies the hypothesis that large firms have more bargaining power with suppliers than do small firms, using data from the cable television industry. Employing techniques from the “new empirical lo,” the effect of owner size on marginal costs is inferred from the effect of owner size on observable product market choices. In the cable industry, the downstream firms decide how many subscriptions of cable to sell and how many channels to offer in the cable package. lf large firms have lower costs than small firms, then large firms should be willing to supply more than small firms, at all prices. The effects of bargaining power are identified separately from the effects of scale economies by exploiting the structure of the cable industry. Scale economies, in the cable industry, are likely to stem from regional size, while bargaining power is likely to stem from national size. By controlling for regional size, estimates of the effect of owner national size on the willingness to supply cable subscriptions and to offer channels indicate that large downstream firms offer significantly more subscriptions and channels at all prices than do small downstream firms. These results provide some of the first systematic, industry-specific, evidence consistent with the bargaining-power hypothesis.

Notes: The role of product warranty in segmentation of consumer durable product markets is highlighted. I demonstrate that consumer moral hazard and heterogeneity in product usage create variation in the valuation of product warranties by the different segments in the market. In this context, the firm, by offering a self-selecting menu of base warranty and extended warranties, satisfies the warranty demands of the various segments of the population. The consumer choice behavior prediction of the theory with regard to extended warranty is empirically validated with data from a survey of new car buyers.

Notes: Research on durable goods has shown that because of a time inconsistency problem, a monopolist manufacturer prefers to rent rather than sell its product. We reexamine the relative profitability of renting versus selling from a marketing perspective. In particular, using a simple linear demand formulation, we assume a durable goods monopolist has to use downstream intermediaries to market its product. In contrast to the case of an integrated monopolist, we find that when the monopolist has to rely on intermediaries, then it prefers to go through an intermediary that sells rather than one that rents its product. Similarly, the intermediary that sells the product is more profitable than the intermediary that rents the product. However, if the monopolist can commit to a set of prices, then the intermediary that rents is more profitable than the intermediary that sells.

Notes: Empirically validating and testing the specification of game theoretic models has received limited attention in the marketing literature. The authors provide an econometric framework for estimating the parameters of response functions when the observed data in the market place are the Nash equilibrium outcomes of an underlying dynamic duopoly game specification. Specifically, the estimation procedure accounts for the joint endogeneity of market shares and marketing efforts of market rivals using a system of simultaneous equations that included the market response function and the Nash equilibrium conditions. A formal statistical test is used to detect model misspecification. The empirical analysis is carried out using data from four product markets: pharmaceutical, soft drink, beer, and detergent. Comparisons are provided with conventional estimation of the response function parameters in which the equilibrium conditions are ignored in the estimation. Managerial implications of the empirical results are discussed.

Notes: Private information creates a cost of operating a hierarchy, which becomes larger as the hierarchical distance between the information source and the decision maker increases. When information about a firm's capabilities is dispersed among the individuals in the firm, production is inefficient even though everyone behaves rationally. Because hierarchies need rents in order to function, a firm with a long hierarchy may not be viable in a competitive industry.

Notes: A dynamic model of product rivalry is developed for a market in which firms choose price and advertising intensity. The model, a state-space game, is implemented using data that consist of weekly price, sales, and promotional activity for four brands of saltine crackers sold by four chains of grocery stores in a small town. A number of questions can be asked of this data. First, is advertising predatory (merely changing market shares) or cooperative (shifting out market demand)? Second, are price and advertising own and cross-strategic complements or substitutes? And finally, do investments in stocks of goodwill and in price reductions make firms tough and aggressive or soft and accommodating?

Notes: Why are moving sales a successful and widespread phenomenon? How can it be optimal for a seller to disclose her low valuation for the item to be sold? We propose an explanation based on the “lemons problem” in bargaining with asymmetric information about quality. Disclosing a low valuation signals that there are significant gains from trade, so that trade takes place when it wouldn't otherwise, and all agents are made better off.

Notes: I present tests of a competitive rationale for price promotions. In a model with a population of informed and uninformed customers, price competition yields a static equilibrium in which each seller draws a price from a specified density function. Price data on coffee and saltine crackers products are used to test whether the sample of prices on each product could have possibly come from the theoretically specified density function. The results suggest that some markets are indeed consistent with the marginal distributions of prices predicted by the model. Furthermore, in the process of testing this rationale for price promotions, estimates are obtained for the marginal cost of each product, the number of competing goods, and the percentage of informed consumers. The resulting excess variability of these estimates across competing brands can also raise questions with respect to the empirical validity of the model.

Notes: It is common in catastrophic food-contamination events that consumers fail to adjust instantaneously to a normal consumption level. One explanation is that consumers only gradually accept new positive information as being trustworthy. The gradual establishment of the trustworthiness of the released information depends on both positive and negative media coverage over time. We examine the individual “trust” effects by extending the prospective reference theory (Viscusi, 1989) to include a dynamic adjustment process of risk perception. Conditions that allow aggregation of changes in risk perceptions across individuals are described. The proposed model describes a general updating process of risk perceptions to media coverage and can be applied to explain the temporal impact of media coverage on consumption of a broad range of goods (food or nonfood). A case study of milk contamination is conducted to demonstrate consumer demand adjustment process to a temporarily unfavorable shock. The results suggest that effects of positive and negative information to adjustment of consumption and risk perception are asymmetric over time.

Notes: The scaling of administered doses to achieve equal degrees of toxic effect in different species has been relatively poorly examined for noncancer toxicity, either empirically or theoretically. We investigate empirical patterns in the correspondence of single oral dose LD, values across several mammalian species for a large number of chemicals based on data reported in the RTECSQ database maintained by the National Institute for Occupational Safety and Health. We find a good correspondence of LD, values across species when the dose levels are expressed in terms of mgadministered per kg of body mass. Our findings contrast with earlier analyses that support scaling doses by the 3/4-power of body mass to achieve equal subacute toxicity of antineoplastic agents. We suggest that, especially for severe toxicity, single- and repeated-dosing regimes may have different cross-species scaling properties, as they may depend on standing levels of defenses and rate of regeneration of defenses, respectively.

Notes: Although occupational exposure limits are sought to establish health-based standards, they do not always give a sufficient basis for planning an indoor air climate that is good and comfortable for the occupants in industrial work rooms. This paper considers methodologies by which the desired level, i.e., target level, of air quality in industrial settings can be defined, taking into account feasibility issues. Risk assessment based on health criteria is compared with risk-assessment based on “Best Available Technology” (BAT). Because health-based risk estimates at low concentration regions are rather inaccurate, the technology-based approach is emphasized. The technological approach is based on information on the prevailing concentrations in industrial work environments and the benchmark air quality attained with the best achievable technology. The prevailing contaminant concentrations are obtained from a contaminant exposure databank, and the benchmark air quality by field measurements in industrial work rooms equipped with advanced ventilation and production technology. As an example, the target level assessment has been applied to formaldehyde, total inorganic dust and hexavalent chromium, which are common contaminants in work room air.

Notes: Cultural Theory, as developed by Mary Douglas, argues that differing risk perceptions can be explained by reference to four distinct cultural biases: hierarchy, egalitarianism, individualism, and fatalism. This paper presents empirical results from a quantitative survey based on a questionnaire devised by Karl Dake to measure these cultural biases. A large representative sample (N = 1022) was used to test this instrument in the French social context. Correlations between cultural biases and perceptions of 20 social and environmental risks were examined. These correlations were very weak, but were statistically significant: cultural biases explained 6%, at most, of the variance in risk perceptions. Standard sociodemographic variables were also weakly related to risk perceptions (especially gender, social class, and education), and cultural biases and sociodemographic variables were themselves inter correlated (especially with age, social class, and political outlook). The authors compare these results with surveys conducted in other countries using the same instrument and conclude that new methods, more qualitative and contextual, still need to be developed to investigate the cultural dimensions of risk perceptions. The paper also discusses relationships between perceptions of personal and residual risk, and between perceived risk and demand for additional safety measures. These three dimensions were generally closely related, but interesting differences were observed for some risk issues.

Notes: The North American Free Trade Agreement (NAFTA) and the General Agreement on Tariffs and Trade (GATT) have focused attention on risk assessment of potential insect, weed, and animal pests and diseases of livestock. These risks have traditionally been addressed through quarantine protocols ranging from limits on the geographical areas from which a product may originate, postharvest disinfestation procedures like fumigation, and inspections at points of export and import, to outright bans. To ensure that plant and animal protection measures are not used as nontariff trade barriers, GATT and NAFTA require pest risk analysis (PRA) to support quarantine decisions. The increased emphasis on PRA has spurred multiple efforts at the national and international level to design frameworks for the conduct of these analyses. As approaches to pest risk analysis proliferate, and the importance of the analyses grows, concerns have arisen about the scientific and technical conduct of pest risk analysis. In January of 1997, the Harvard Center for Risk Analysis (HCRA) held an invitation-only workshop in Washington, D.C. to bring experts in risk analysis and pest characterization together to develop general principles for pest risk analysis. Workshop participants examined current frameworks for PRA, discussed strengths and weaknesses of the approaches, and formulated principles, based on years of experience with risk analysis in other setting and knowledge of the issues specific to analysis of pests. The principles developed highlight the both the similarities of pest risk analysis to other forms of risk analysis, and its unique attributes.

Notes: A substantial body of risk research indicates that women and men differ in their perceptions of risk. This paper discusses how they differ and why. A review of a number of existing empirical studies of risk perception points at several problems, regarding what gender differences are found in such studies, and how these differences are accounted for. Firstly, quantitative approaches, which have so far dominated risk research, and qualitative approaches give different, sometimes even contradictory images of women's and men's perceptions of risk. Secondly, the gender differences that appear are often left unexplained, and even when explanations are suggested, these are seldom related to gender research and gender theory in any systematic way. This paper argues that a coherent, theoretically informed gender perspective on risk is needed to improve the understanding of women's and men's risk perceptions. An analysis of social theories of gender points out some relations and distinctions which should be considered in such a perspective. It is argued that gender structures, reflected in gendered ideology and gendered practice, give rise to systematic gender differences in the perception of risk. These gender differences may be of different kinds, and their investigation requires the use of qualitative as well as quantitative methods. In conclusion, the arguments about gender and risk perception are brought together in a theoretical model which might serve as a starting point for further research.

Notes: The AC electric and magnetic fields associated with high voltage power lines have become a concern as a possible health risk. In most cases the strength of these fields decreases as the inverse square of the distance from the line. In earlier work, we found that laypeople do not understand how rapidly field strength decreases with distance. Most believe that any high voltage power line they can see is exposing them to strong fields. This paper confirms the earlier finding and explores a number of strategies which might be used in risk communications to correct this misperception. We found it relatively easy to provide subjects with a better understanding of the range-dependency of magnetic field strength. Moreover, the quality of this acquisition was apparently independent of the manner in which they were instructed. Such successful instruction is markedly different from the well-established difficulty of teaching people about many qualitative domains, such as physics or ideas in probability. Clearly, while some erroneous beliefs are highly resistant to change, others can be altered quite readily. We suspect that an important distinction between knowledge about the range-dependency of power-frequency magnetic fields and less tractable topics involves the presence or absence of prior folk-theories or “mental models” of the domain.

Notes: In the spring of 1993, about 39% of Milwaukee-area residents suffered through a nationally publicized illness brought about by cryptosporidium, a parasite that had infested the metropolitan drinking water supply. Our study, based on a telephone survey of 610 local adult residents, indicates that worry about becoming ill in the future with cryptosporidiosis relates more strongly and consistently to public reliance on, and use of, media for cryptosporidium information than do a range of risk perception and experience variables. We propose that more studies should take an audience-centered approach to understanding risk communication.

Notes: This paper seeks to compare two frameworks which have been proposed to explain risk perceptions, namely, cultural theory and the psychometric paradigm. A structured questionnaire which incorporated elements from both approaches was administered to 129 residents of Norwich, England. The qualitative risk characteristics generated by the psychometric paradigm explained a far greater proportion of the variance in risk perceptions than cultural biases, though it should be borne in mind that the qualitative characteristics refer directly to risks whereas cultural biases are much more distant variables. Correlations between cultural biases and risk perceptions were very low, but the key point was that each cultural bias was associated with concern about distinct types of risks and that the pattern of responses was compatible with that predicted by cultural theory. The cultural approach also provided indicators for underlying beliefs regarding trust and the environment; beliefs which were consistent within each world view but divergent between them. An important drawback, however, was that the psychometric questionnaire could only allocate 32% of the respondents unequivocally to one of the four cultural types. The rest of the sample expressed several cultural biases simultaneously, or none at all. Cultural biases are therefore probably best interpreted as four extreme world views, and a mixture of qualitative and quantitative research methodologies would generate better insights into who might defend these views in what circumstances, whether there are only four mutually exclusive world views or not, and how these views are related to patterns of social solidarity, and judgments on institutional trust.

Notes: Public responses to nuclear technologies are often strongly negative. Events, such as accidents or evidence of unsafe conditions at nuclear facilities, receive extensive and dramatic coverage by the news media. These news stories affect public perceptions of nuclear risks and the geographic areas near nuclear facilities. One result of these perceptions, avoidance behavior, is a form of “technological stigma” that leads to losses in property values near nuclear facilities. The social amplification of risk is a conceptual framework that attempts to explain how stigma is created through media transmission of information about hazardous places and public perceptions and decisions. This paper examines stigma associated with the U.S. Department of Energy's Rocky Flats facility, a major production plant in the nation's nuclear weapons complex, located near Denver, Colorado. This study, based upon newspaper analyses and a survey of Denver area residents, finds that the social amplification theory provides a reasonable framework for understanding the events and public responses that took place in regard to Rocky Flats during a 6-year period, beginning with an FBI raid of the facility in 1989.

Notes: This paper presents benchmark (BMD) calculations and additional regression analyses of data from a study in which scores from 26 scholastic and psychological tests administered to 237 6- and 7-year-old New Zealand children were correlated with the mercury concentration in their mothers' hair during pregnancy. The original analyses of five test scores found an association between high prenatal mercury exposure and decreased test performance, using category variables for mercury exposure. Our regression analyses, which utilized the actual hair mercury level, did not find significant associations between mercury and children's test scores. However, this finding was highly influenced by a single child whose mother's mercury hair level (86 mgkg) was more than four times that of any other mother. When that child was omitted, results were more indicative of a mercury effect and scores on six tests were significantly associated with the mothers' hair mercury level. BMDs calculated from five tests ranged from 32 to 73 mgkg hair mercury, and corresponding BMDLs (95% lower limits on BMDs) ranged from 17 to 24 mgkg. When the child with the highest mercury level was omitted, BMDs ranged from 13 to 21 mgkg, and corresponding BMDLs ranged from 7.4 to 10 mgkg.

Notes: Truck transport of radioactive material (RAM), e.g., spent nuclear fuel (SNF), normally maximizes use of Interstate highways, which are safer and more efficient for truck transport in general. In the estimation of transportation risks, population bordering a route is a direct factor in determining consequences and an indirect factor in determining exposure times, accident probabilities and severities, and other parameters. Proposals to transport RAM may draw intense resistance from “stakeholders” based on concern for population concentrations along urban segments but the length of a route segment is also a determinative factor in estimating the transport risks. To quantify the relative importance of these two factors, a potential route for transport of SNF (strict use of Interstate highways) was selected and compared with a modified version that bypassed urban areas. The results suggest that emphasis on Interstate highways minimizes total route and urban segment risks.

Notes: In 1984, based on epidemiological data on cohorts of coke oven workers, USEPA estimated a unit risk for lung cancer associated with continuous exposure from birth to 1 pg/m3 of coke oven emissions, of 6.2 × This risk assessment was based on information on the cohorts available through 1966. Follow-up of these cohorts has now been extended to 1982 and, moreover, individual job histories, which were not available in 1984, have been constructed. In this study, lung cancer mortality in these cohorts of coke oven workers with extended follow-up was analyzed using standard techniques of survival analysis and a new approach based on the two stage clonal expansion model of carcinogenesis. The latter approach allows the explicit consideration of detailed patterns of exposure of each individual in the cohort. The analyses used the extended follow-up data through 1982 and the detailed job histories now available. Based on these analyses, the best estimate of unit risk is 1.5 × with 95% confidence interval = 1.2 × 10-”1.8 X

Notes: This project describes a methodology for assessing relative risk along a transportation corridor utilizing waterborne transportation on the busiest port area in the world, the lower Mississippi River (from the mouth of Southwest Pass up through Baton Rouge, Louisiana). The paper calculates a relative risk scale, using data obtained from maritime experts, previous research, and existing databases. The research aggregates the vessel traffic data and geographic risk location data to produce relative risk scores for each mile along the River from the mouth of Southwest Pass to the termination of shipping at the U.S. 190 bridge across the River at Baton Rouge. This is done in a very simple and practical way for this initial model: (1) each vessel traveling the Mississippi is classified according to its risk potential for those miles that it passes in route to where it docks, and (2) points along the river are assigned a relative risk score based upon risk variables identified by expérts identified through a standard sampling procedure. The relative risk scores for river miles are combinations of these two factors.

Notes: Real-world exposure measurements are a necessary ingredient for subsequent detailed study of the risks from an environmental pollutant. For volatile organic compounds, researchers are applying exhaled breath analysis and the time dependence of concentrations as a noninvasive indicator of exposure, dose, and blood levels. To optimize the acquisition of such data, samples must be collected in a time frame suited to the needs of the mathematical model, within physical limitations of the equipment and subjects, and within logistical constraints. Additionally, one must consider the impact of measurement error on the eventual extraction of biologically and physiologically relevant parameters. Given a particular mathematical model for the elimination kinetics (in this case a very simple pharmacokinetic model based upon a multitenn exponential decay function that has been shown to fit real-world data extremely well), we investigated the effects on synthetic data caused by sample timing, random measurement error, and number of terms included in the model. This information generated a series of conditions for collecting samples and performing analyses dependent upon the eventual informational needs, and it provided an estimate of error associated with various choices and compromises. Though the work was geared specifically toward breath sampling, it is equally applicable to direct blood measurements in optimizing sampling strategy and improving the exposure assessment process.

Notes: Health risks from fossil, renewable and nuclear reference energy systems are estimated following a detailed impact pathway approach. Using a set of appropriate air quality models and exposure-effect functions derived from the recent epidemiological literature, a methodological framework for risk assessment has been established and consistently applied across the different energy systems, including the analysis of consequences from a major nuclear accident. A wide range of health impacts resulting from increased air pollution and ionizing radiation is quantified, and the transferability of results derived from specific power plants to a more general context is discussed.

Notes: This paper investigates electrical overheating events aboard a habitable spacecraft. The wire insulation involved in these failures plays a major role in the entire event scenario from threat development to detection and damage assessment. Ideally, if models of wire overheating events in microgravity existed, the various wire insulations under consideration could be quantitatively compared. However, these models do not exist. In this paper, a methodology is developed that can be used to select a wire insulation that is best suited for use in a habitable spacecraft. The results of this study show that, based upon the Analytic Hierarchy Process and simplifying assumptions, the criteria selected, and data used in the analysis, Tefzel is better than Teflon for use in a habitable spacecraft.

Notes: Abstract Phase relations and mineral chemistry for garnet (Grt), orthopyroxene (Opx), sapphirine (Spr), water-undersaturated cordierite (Crd), osumilite (Osu), sillimanite (Sil), K-feldspar (Kfs), quartz (Qtz) and a water-undersaturated liquid (Liq) have been determined experimentally in the system KFMASH (K2O-FeO-MgO-Al2O3-SiO2-H2O) under low PH2O and fO2 conditions. Four compositions have been studied with 100 [Mg/(Mg + Fe)] ranging from 65.6 to 89.7. Based on our experimental data, a P-T grid is derived for the KFMASH system in the presence of quartz, orthopyroxene and liquid. Osumilite has been found in various mineral assemblages from 950 to 1100°C and 7.5 to 11 kbar. In the temperature range 1000-1100°C, the pair Os-Grt is stable over a pressure range of about 3kbar. The divariant reaction Os + Opx = Grt + Kfs + Qtz runs to the right with increasing pressure. Because osumilite is the most magnesian phase it is restricted to Mg-rich compositions at high pressure. The reaction defining the upper pressure stability limit of Os-Grt is located around 11 kbar with a nearly flat dP/dT slope over the temperature range 950–100°C. Over the entire temperature range investigated osumilite is not stable beyond 12 kbar. The data imply a restricted pressure range between 11 and 12 kbar for the stability of the assemblage Os-Opx-Sil-Kfs-Qtz. At 1050°C and above, osumilite occurs in various mineral assemblages together with the high-T pair Spr-Qtz.When coexisting with garnet, orthopyroxene or sapphirine, osumilite is always the most magnesian phase. At 1050 and 1100°C, liquid is invariably the most Fe-rich phase in the run product.Our data support a theoretical P-T grid for the KFMAS system in which osumilite is stable outside the field of the high-T assemblage Spr-Qtz. Moreover, our grid indicates that Os-Opx-Sil-Kfs-Qtz has a more restricted pressure and compositional stability domain than Os-Grt, in agreement with natural occurrences. Osumilite is stable over a large pressure range, such that in Mg-rich rocks, and at high temperature, it can occur at any depth in normal thickness continental crust.

Notes: Abstract Mg-gabbros from East Ligurian ophiolites (Northern Apennines, Italy) display a high-temperature/low-pressure recrystallization localized along ductile shear zones. In deformed gabbros, the igneous diopside is recrystallized into granoblastic aggregates of neoblastic diopside and minor red-brown amphibole. The latter displays a pargasitic composition, with high amounts of AlIV Na(A) and Ti (± 1.8, 0.7 and 0.4 atoms per formula unit, respectively). Major element composition of neoblastic minerals highlight equilibration temperature conditions in the range 800–950° C. Red-brown Ti-pargasite also occurs as a minor interstitial constituent, presumably growing from a residual trapped liquid, in the differentiated lithologies (Fe-Ti-diorites) of the plutonic ophiolitic complex.By means of ion microprobe (SIMS technique), rare earth (La, Ce, Nd, Sm, Eu, Gd, Dy, Er, Yb) and selected trace elements (Sr, Y, Cr, V, Sc, Zr, Ti) have been analysed in igneous and neoblastic diopside, as well as in Ti-pargasites. Ti-pargasites have also been analysed for F and Cl, and compared with the halogen composition of the amphiboles, mainly hornblendes to actinolites, which are related to the subsequent low-temperature brittle evolution.Neoblastic Ti-pargasite from deformed Mg-gabbros bears close compositional similarities with igneous Ti-pargasite from undeformed Fe-Ti-diorites, whereas it is geochemically distinct from the amphiboles post-dating the ductile event. In particular, Ti-pargasites have relatively high contents of F, REE, Y, Zr and Sr, which are not consistent with crystallization in the presence of seawater-derived hydrothermal fluids.High-grade recrystallization probably developed in the presence of volatile-rich igneous fluids, either trapped between the cumulus minerals or injected into the ductile shear zones from outside the local system. An alternative hypothesis comprises the absence of fluid phase and the development of ductile shear zones in Ti-pargasite-rich layers. The petrological features of deformed gabbros recovered from present-day slow-spreading ridges and fracture zones bear close similarities with the investigated ophiolitic metagabbros. In East Ligurian ophiolites, high-grade ductile shear zones have been related to the initial stages of the uplift of the gabbro-peridotite complex to the sea-floor.

Notes: Abstract A petrographic and petrological analysis of exceptionally well-preserved hydrothermal veins from the Merrimack synclinorium, north-eastern Connecticut, has been carried out in order to place new field-based constraints on fracture aperture dimensions and porosity in the lower continental crust. The veins preserve substantial open space today in outcrop, and contain mineral assemblages including subhedral to euhedral crystals of quartz, kyanite and almandine-rich garnet. Textural evidence indicates unequivocally that the vein minerals grew into macroscopic (mm- to cm-scale) open space between the vein walls. The veins are interpreted to have been large-aperture fractures along which significant advective fluid infiltration and chemical reaction occurred. The porosity of the rock mass due to open space between fracture walls today is c. 0.3%, but it could have been as large as several percent when the flow system was active. Quantitative thermobarometry results from vein mineral assemblages indicate that the fractures formed at pressures and corresponding crustal depths of c. 0.8 GPa and c. 30km, and temperatures of 550–600° C. The depth of fracture formation corresponds to published estimates of the maximum burial depth of the Merrimack synclinorium during the Acadian orogeny. The formation of large-aperture fractures could increase significantly the transient permeability of the deep crust, and therefore influence metamorphic heat and mass transfer.

Notes: Abstract 40Ar/39Ar ages from white mica in rocks of the internal zone of the Brooks Range contractional orogen indicate that the Nanielik antiformal duplex developed at about 120 Ma and was remobilized on its southern boundary at c. 108 Ma. Blueschist facies metamorphism accompanied development of the antiform. The timing of the blueschist facies event and creation of the antiform overlap the period of shallow-seated deformation in the foreland fold and thrust belt and sedimentation in the foreland basin of the Brooks Range. Blueschist facies P-T conditions may therefore characterize the thicker parts of orogenic wedges in some orogenic systems; ancient blueschists need not necessarily be interpreted as indicators of active subduction or continent-continent collision.Microprobe analysis using quantitative wavelength-dispersive and electron backscattered electron imaging methods was used to characterize the composition of white micas in the dated samples. None of the samples was compositionally homogeneous; many contained 2-3 populations of white mica, including both potassic and sodic varieties. Samples which had undergone (in sequence) amphibolite, albite-epidote amphibolite and blueschist facies metamorphic events retained muscovites relict of the amphibolite facies event. Samples that had undergone only the blueschist facies event also contained multiple populations of mica, some probably from detrital sources.

Notes: Abstract Ductilely deformed amphibolite facies tectonites comprise two adjacent terranes in east-central Alaska. These terranes differ in protoliths, structural level and cooling ages. A structurally complex zone of gently north-dipping tectonites separates the two terranes. The northern, structurally higher Taylor Mountain terrane includes garnet amphibolite, biotite ± hornblende gneiss, marble, quartzite, metachert, pelitic schist and cross-cutting granitoids of intermediate composition (including the Late Triassic to Early Jurassic Taylor Mountain batholith). Lithological associations and isotopic data from the granitoids indicate an oceanic or marginal basin origin for the Taylor Mountain terrane. 40Ar/39Ar metamorphic cooling ages from the Taylor Mountain terrane are latest Triassic to earliest Middle Jurassic. The southern, structurally lower Lake George subterrane of the Yukon-Tanana terrane is made up of quartz-biotite schist and gneiss, augen gneiss, pelitic schist, garnet amphibolite and quartzite; we interpret it to comprise a continental margin and granitoid belt built on North American crust. Metamorphic cooling ages from the Lake George subterrane are almost entirely Early Cretaceous.Geothermobarometric analysis of garnet rims and adjacent phases in garnet amphibolite and pelitic schist from the Taylor Mountain terrane and Lake George subterrane indicate peak metamorphic conditions of 7.5-12 kbar at 555-715° C in the northern part of the Taylor Mountain terrane, in which NNE-vergent shear fabrics are preserved; 6.5-10.8 kbar at 520-670° C within the contact zone between the two terranes, in which NW-vergent shear fabrics predominate; and 6.8-11.8 kbar at 570-700° C in the Lake George subterrane of the Yukon-Tanana terrane, in which NW-vergent shear is recorded in the northern part of the study area and SE-vergent shear in the southern part. Where the two shear-sense directions occur together in the northern Lake George subterrane and, locally, in the contact zone, fabrics that record NW-vergent shear are more penetrative and preceded fabrics that record SE-vergent shear.We interpret the pressure, temperature, kinematic and age data to indicate that the metamorphism of the Taylor Mountain terrane and Lake George subterrane took place during different phases of a latest Palaeozoic through early Mesozoic shortening episode resulting from closure of an ocean basin now represented by klippen of the Seventymile-Slide Mountain terrane. High- to intermediate-pressure metamorphism of the Taylor Mountain terrane took place within a SW-dipping (present-day coordinates) subduction system. High- to intermediate-pressure metamorphism of the Lake George subterrane and the structural contact zone occurred during NW-directed overthrusting of the Taylor Mountain, Seventymile-Slide Mountain and Nisutlin terranes, and imbrication of the continental margin in Jurassic time. The difference in metamorphic cooling ages between the Taylor Mountain terrane and adjacent parts of the Lake George subterrane is best explained by Early Cretaceous unroofing of the Lake George subterrane caused by crustal extension, recorded in its younger top-to-the-SE fabric.

Notes: Abstract High-P/T metamorphic parageneses are preserved within two late Palaeozoic to early Mesozoic assemblages of the southern Klamath Mountains that show contrasting structural styles and mineral parageneses reflecting formation in different parts of a subduction-zone regime. Blueschist facies tectonites of the Stuart Fork terrane represent a coherent subduction complex formed at relatively deep crustal levels, whereas the chaotic metasedimentary mélange of the eastern Hayfork terrane contains a diverse range of metamorphic parageneses reflecting complex structural mixing of metamorphic components at shallower levels. The convergent-margin-type accretionary metamorphism evident in both terranes pre-dates Middle Jurassic low-P/T metamorphism resulting from regional tectonic contraction and magmatism.The epidote- to lawsonite-zone Stuart Fork blueschists (and eclogites locally) formed at pressures of about 6-11 kbar and temperatures of 250-400° C. Deformed matrix material of the eastern Hayfork mélange formed at similar temperatures but lower pressures, on the order of 3-6 kbar. The mélange contains a diverse assemblage of tectonic blocks that formed under a range of P-T conditions, including those of the blueschist, pumpellyite-actinolite, greenschist and upper greenschist to amphibolite facies.The succession of mineral parageneses and inferred P-T conditions of the eastern Hayfork blocks reflect those of igneous protolith formation, structural mixing, subduction-zone metamorphism, olistolith transport, and tectonic and erosional denudation. Although temporal relations are not well constrained, the evolution of these terranes is consistent with formation within a single convergent-margin system.

Notes: Abstract In the Su-Lu ultrahigh-P terrane, eastern China, many coesite-bearing eclogite pods and layers within biotite gneiss occur together with interlayered metasediments now represented by garnet-quartz-jadeite rock and kyanite quartzite. In addition to garnet + omphacite + rutile + coesite, other peak-stage minerals in some eclogites include kyanite, phengite, epidote, zoisite, talc, nyböite and high-Al titanite. The garnet-quartz-jadeite rock and kyanite quartzite contain jadeite + quartz + garnet + rutile ± zoisite ± apatite and quartz + kyanite + garnet + epidote + phengite + rutile ± omphacite assemblages, respectively. Coesite and quartz pseudomorphs after coesite occur as inclusions in garnet, omphacite, jadeite, kyanite and epidote from both eclogites and metasediments. Study of major elements indicates that the protolith of the garnet-quartz jadeite rock and the kyanite quartzite was supracrustal sediments. Most eclogites have basaltic composition; some have experienced variable ‘crustal’contamination or metasomatism, and others may have had a basaltic tuff or pyroclastic rock protolith.The Su-Lu ultrahigh-P rocks have been subjected to multi-stage recrystallization and exhibit a clockwise P-T path. Inclusion assemblages within garnet record a pre-eclogite epidote amphibolite facies metamorphic event. Ultrahigh-P peak metamorphism took place at 700–890° C and P〉28 kbar at c. 210–230 Ma. The symplectitic assemblage plagioclase + hornblende ± epidote ± biotite + titanite implies amphibolite facies retrogressive metamorphism during exhumation at c. 180–200 Ma. Metasedimentary and metamafic lithologies have similar P-T paths. Several lines of evidence indicate that the supracrustal rocks were subducted to mantle depths and experienced in-situ ultrahigh-P metamorphism during the Triassic collision between the Sino-Korean and Yangtze cratons.

Notes: Abstract Blueschists occurring as layers in calcite marbles of the Meliata unit occur along the so-called Roznava tectonic line situated in the southern part of the Gemericum, Slovakia. Mineral assemblages and compositions from seven blueschists localities and one occurrence of amphibolite facies rocks overprinted by blueschist metamorphism were investigated. The most common minerals in the blueschists are blue amphibole, epidote and albite. Some Fe2+- and Al-rich rocks also contain garnet and chloritoid, respectively. Na-pyroxene with a maximum 50% jadeite component was also found. The blue amphiboles correspond mostly to crossite and also to glaucophane and ferroglaucophane in some samples. Almandine- and spessartine-rich garnet has very low MgO content (〈3 wt%). The Si content in phengite ranges between 3.3 and 3.5 pfu calculated on the basis of 11 oxygens. The zoning patterns of blue amphibole, garnet and chloritoid suggest their formation during a prograde stage of metamorphism. The P-T conditions of metamorphism are estimated to be about 380–460° C and 10–13 kbar. Pressures of 7.5–8.5 kbar and temperatures of 350–370° C were obtained for some actinolite- and aegirine-rich rocks. Apart from chlorite, other mafic minerals formed during retrograde metamorphism are biotite and occasionally also actinolite.

Notes: Abstract The Port aux Basques gneisses comprise three lithostratigraphic units separated by major fault zones: the Grand Bay Complex; the Port aux Basques Complex; and the Harbor le Cou Group. A similar regionally developed polyphase history of penetrative deformation characterizes each of these units. Thickening during D1 produced rare recumbent folds (F1) and an axial planar schistosity (S1), overprinted by D2 recumbent folds (F2), and transposed during development of a locally penetrative, differentiated crenulation cleavage (S2). In western sectors of the area, D2 was associated with NW-directed reverse shearing. The NE-trending structural grain reflects D3 transpression, partitioned into dextral transcurrent movement along major shear zones and development of upright-to-steeply inclined, periclinal folds (F3) and a variably penetrative schistosity (S3).Amphibolite facies metamorphism increases in grade from west to east across the area. Microstructures, including porphyroblast-matrix foliation relations and internal textural unconformities in garnet, indicate episodic porphyroblast nucleation and growth, which reflect a prograde traverse sequentially across univariant reactions during syntectonic metamorphism. Garnet, kyanite and staurolite porphyroblasts are wrapped by the S2 foliation, but each may contain trails of inclusions that define S1; commonly these trails preserve early stages of S2 crenulation cleavage development. Progressive and sequential reaction out of kyanite, staurolite and muscovite in favour of sillimanite, garnet, biotite and K-feldspar, and the development of an increasing volume of anatectic migmatite in south-eastern sectors of the area record syn- to late-D2 peak metamorphic conditions. Microstructural relationships and petrogenetic grid considerations indicate clockwise trajectories in P-T space for units of the Port aux Basques gneisses. Peak metamorphic conditions are estimated to have been 620–650° C at ≤8kbar in the west and 700–750° C at ≤8 kbar in the east.Titanite from an upper amphibolite facies calc-silicate gneiss yields U-Pb ages of c. 420 Ma, interpreted to date cooling shortly after the thermal peak in these gneisses. Variable D3 strain was associated with some recrystallization of hornblende and micas. 40Ar/39Ar hornblende plateau isotope correlation ages range from c. 419 to c. 393 Ma, from east to west across the area, and are interpreted to record cooling through c. 500° C coeval with or soon after D3 deformation. The range in ages may record the effects of heterogeneous D3 deformation and differential uplift from south-east to north-west associated with displacement on major shear zones. 40Ar/39Ar muscovite plateau ages cluster at c. 390 Ma, and date cooling through c. 375° C during regional exhumation. Cooling rates are moderate to fast and may indicate a component of tectonic exhumation.The Port aux Basques gneisses are a product of Silurian collisional tectonics. The higher grade of metamorphism in comparison with adjacent areas of the Canadian Appalachians is interpreted to reflect greater thickening due to juxtaposition of the St Lawrence promontory (Laurentian margin) with the Cabot promontory (Avalonian margin) during closure of the Iapetus Ocean.

Notes: Abstract Carbon isotope thermometry has been applied to coexisting calcite and graphite in marbles from throughout the Adirondack Mountains, New York. Eighty-nine calcite-graphite pairs from the amphibolite grade NW Adirondacks change systematically in temperature north-westwards from 680 to 640 to 670° C over a 30-km distance, reflecting transitions from amphibolite facies towards granulite facies to the north-west and to the south-east. Temperature contours based on calcite-graphite thermometry in the NW Adirondacks parallel mineral isograds, with the orthopyroxene isograd falling above 675° C, and indicate that regional metamorphic temperatures were up to 75° C higher than temperatures inferred from isotherms based on cation and solvus thermometry (Bohlen et al. 1985). Fifty-five calcite-graphite pairs from granulite grade marbles of the Central Adirondacks give regional metamorphic temperatures of 670–780° C, in general agreement with cation and solvus thermometry.Data for amphibolite and granulite grade marbles show a 12%oo range in δ13Ccal and δ13Cgr. A strong correlation between carbon isotopic composition and the abundance of graphite (Cgr/Crock) indicates that the large spread in isotopic compositions results largely from exchange between calcite and graphite during closed system metamorphism. The trends seen in δ13C vs. Cgr/Crock and δ13Ccal vs. δ13Cgr could not have been preserved if significant amounts of CO2-rich fluid had pervasively infiltrated the Adirondacks at any time. The close fit between natural data and calculated trends of δ13C vs. Cgr/Crock indicates a biogenic origin for Adirondack graphites, even though low δ13C values are not preserved in marble.Delamination of 17 graphite flakes perpendicular to the c-axis reveals isotopic zonation, with higher δ13C cores. These isotopic gradients are consistent with new graphite growth or recrystallization during a period of decreasing temperature, and could not have been produced by exchange with calcite on cooling due to the sluggish rate of diffusion in graphite. Samples located 〉2km from anorthosite show a decrease of 0.5-0.8%oo in the outer 100 μ of the grains, while samples at distances over 8 km show smaller core-to-rim decreases of c.0.2%oo. Correlation between the degree of zonation and distance to anorthosite suggests that the isotopic profiles reflect partial overprinting of higher temperature contact metamorphism by later granulite facies metamorphism. Core graphite compositions indicate contact metamorphic temperatures were 860–890° C within 1 km of the Marcy anorthosite massif. If samples with a significant contact metamorphic effect (Δ(cal-gr) 〈3.2%oo) are not included, then the remaining 38 granulite facies samples define the relation Δ13C(cal-gr) = 3.56 ± 106T-2 (K).

Notes: Native grasses may be used for multiple, sometimes conflicting, goals in revegetation projects. Woody plants are frequently desired for moose browse and timber in Alaska, but naturally occurring Calamagrostis canadensis (bluejoint reedgrass) hinders the establishment of these desired species. Seven grass cultivars of Alaskan origin were evaluated for their ability (1) to stabilize the soil, (2) to reduce regeneration of C. canadensis, (3) to allow openings for natural colonization, and (4) to permit establishment of desirable rooted cuttings. Cultivars tested are “Arctared” Festuca rubra (red fescue), “Alyeska”Arctagrostis latifolia (polar-grass), “Nugget”Poa pratensis (bluegrass) “Norcoast”Deschampsia beringensis (Bering hairgrass), “Nortran”Deschampsia caespitosa (tufted hairgrass), “Gruening” Poa alpina (alpine bluegrass), and “Sourdough” Calamagrostis canadensis. These were tested as single species and in multi-species mixtures, with two seeding rates of the multi-species mixture (0.5, 0.25 seeds/cm2). Experimental plots included unfertilized, unfertilized with rooted Salicaceae cuttings, and fertilized (350 kg/ha 20:20:10). A control plot was not seeded. After three growing seasons, Nortran D. caespitosa and Arctared F. rubra were the most successful cultivars. They provided 87% to 98% of the seeded-species cover for soil stabilization and suppressed C. canadensis on the fertilized subplots without reducing species diversity. Gruening Poa alpina was less than 3 cm tall, and it helped stabilize the site without interfering with woody plant establishment. Although cuttings were shorter under some seed treatments compared to the nonseeded control, heights of cuttings were not related to cover of seeded cultivars (r = 0.09, p 〉 0.55) but were positively correlated with total vascular plant cover (r= 0.61, p 〈 0.001).

Notes: Throughout tropical moist climates, Dicranopteris linearis fernlands can develop as a result of rain forest clearance followed by frequent burning. In Sri Lanka, D. linearis fern-lands are capable of suppressing the regeneration of rain forest. Field experiments were conducted at Sinharaja Man and Biosphere Reserve, a rain forest where fernlands occupy substantial areas of the reserve boundary. The experiment's objective was to identify methods for initiating forest regeneration in fernlands dominated by D. linearis Three disturbance treatments were used to initiate seedling regeneration: clean weed, root removal, and till. We hypothesized that increasing the severity of the soil disturbance would establish vegetation with higher species richness and diversity, greater above-ground dry biomass, and higher percentage cover and seedling density. Results indicate only partial support for this hypothesis. Dry biomass was greatest in till treatments, the most severe soil disturbance. By comparison, species richness and diversity, seedling density, and percentage cover were greatest in root-removal treatments, though in many instances the differences were not significant. The study clearly demonstrated that any kind of soil disturbance can facilitate the establishment of herbs, shrubs, and trees in a fernland dominated by D. linearis. Results showed that herbs, sedges, grasses, and pioneer shrubs represented greater proportions of seedling recruits than did pioneer trees. Seedlings of primary-forest tree species were nearly nonexistent. In general, results showed that soil disturbance can play an important role in site preparation for the purpose of initiating non-fern vegetation in fernlands dominated by D. linearis.

Notes: The effect of a pipeline corridor constructed through an ecological reserve in Southern California was investigated by assessing plant species composition and soil chemistry. A homogeneous plant community comprised primarily of exotic annuals was found along the entire length of the corridor. This community has low similarity to the adjacent native plant communities. Soil organic matter was significantly less on the disturbed corridor than in contiguous undisturbed areas. Both available nitrogen and extractable phosphorus values were greater in the disturbed corridor. By contrast, total nitrogen was significantly higher outside the pipeline. The more labile litter of the exotic annuals allows increased mineralization along the corridor than does the more recalcitrant litter of the native perennial shrubs in the undisturbed areas. Once established, the weedy exotic annual litter may completely turn over organic matter and nitrogen, favoring the persistence of the weedy annuals. These exotic annuals appear to be moving into three of the native communities - grassland, coastal sage, and oak woodland - that have less organic matter and a more open plant canopy. Poor restoration efforts can lead to the establishment of such exotics, subsequent invasion into the surrounding undisturbed habitat, and degradation of the reserve.

Notes: A critical element of the ongoing effort to restore the ecological integrity of Florida's Kissimmee River ecosystem is the reestablishment of pre-channelization habitat structure and function. Restoration of habitat will form the basis for responses by most biological components of the ecosystem and will provide a key indicator of the success of the restoration effort. This paper evaluates the relative importance of a range of abiotic and biotic habitat parameters in the existing and historic Kissimmee River ecosystem and provides a conceptual framework for predicting expected spatial and temporal responses of river and floodplain habitats to the restoration project. Among the ecological factors and process that influenced the development, dynamics, and maintenance of river and floodplain habitat structure, hydrology is expected to be of central importance in eliciting restoration responses in the Kissimmee River Ecosystem. Based on the assumption that the restoration plan will reestablish historic hydrologic characteristics, predictions are made of expected responses by geomorphic and vegetative components of the Kissimmee River's habitat structure. Recommendations are made regarding key habitat parameters requiring long term tracking and analysis and utilization of a geographic information system(GIS). A hierarchical habitat classification scheme is provided as a foundation for all components of the restoration evaluation program.

Notes: When the Kissimmee River was channelized in the 1960s and 1970s and placed under stage-fluctuation management, the dynamic interactions between the river and the flood-plain were essentially removed. Correspondingly, aquatic invertebrate life in the river and floodplain ecosystem shifted from a riverine to a more lacustrine fauna. A relinkage of the Kissimmee River with the floodplain following restoration will result in numerous changes to such ecologically important factors as streamflow, substrate composition, food quality and quantity, and water quality, all of which will influence invertebrate communities. These factors and their function in the ecosystem as the fauna shifts from predominantly lacustrine back to riverine are presented in a conceptual model. As an integral component of all aquatic ecosystems and a key link between primary producers and higher trophic levels, aquatic invertebrates are a valuable group with which to evaluate the recovery of the Kissimmee River. Utilization of a geographic information system mapping approach linking expected increased habitat heterogeneity and invertebrate richness with restoration efforts is suggested as an economical means of monitoring recovery of the Kissimmee River ecosystem.

Notes: Despite the critical role insects play in ecosystem functioning, there has been little study of factors affecting their reestablishment in restored ecosystems. The goals of this research were to quantify the nectar resources provided by reclaimed coal surface mines and to examine the role nectar resources play in determining butterfly community composition on these sites. Adult butterfly communities and nectar resources were sampled on 18 reclaimed coal surface-mined sites and five unmined hardwood sites in southwestern Virginia. Recently, reclaimed sites provided an average of 300 times the nectar abundance of the surrounding hardwoods, and nectar abundance and species richness decreased with time since reclamation. Total nectar abundance was highly correlated with total butterfly abundance and species richness for the entire flight season; these variables were also significantly correlated among sites during most of the 12 sampling periods during the flight season. In only a few cases, however, were butterfly and nectar abundance and species richness significantly correlated within individual sites during the flight season. These results suggest that, although adults of many butterfly species move in response to nectar availability, nectar resources are not sufficiently limiting that their life histories have evolved to maximize nectar resources temporally. While planting species in restored areas that provide abundant nectar will likely attract adult butterflies, this is only one of a number of habitat variables that must be considered in efforts to restore butterfly populations. Finally, adult butterflies appear to have limited utility as indicators of revegetation success.

Notes: The restoration of the northern jarrah (Eucalyptus marginata) forest after bauxite mining is a major objective of Alcoa of Australia Limited. The typically variable and sometimes low emergence of broadcast seed of jarrah-forest plant species may relate to microclimatic changes associated with mining disturbance. This study examined the effect of the presence and absence of a canopy and topographic position in the post-mining landscape on the emergence of four canopy species (E. marginata, E. calophylla, E. patens, and E. diversicolor) and related these patterns to detailed measures of surface soil temperature and moisture. The absence of a canopy in the restoration appeared to result in adverse microclimatic conditions for the successful early establishment of E. marginata and E. calophylla from seed, particularly in the low topographic regions of the restoration. Emergence beneath a canopy compared to that in the open was 17% and 6%, respectively, for E. marginata and 23% and 2%, respectively, for E. calophylla. For both species, emergence was also greater at upland than at lowland open restoration sites (9% and 3%, respectively, for E. marginata; 4% and 0.3%, respectively, for E. calophylla). In contrast, canopy removal and position on the topographic landscape did not reduce the early establishment success of E. patens and E. diversicolor. Field measurements revealed that soils were drier and that diurnal temperature fluctuations were wider in the open restoration sites than beneath a canopy. Furthermore, cold conditions were more frequent at lowland than at upland restoration sites, suggesting the occurrence of cold-air drainage to Jew-lying areas. It is therefore possible that the field emergence patterns reflected the lower tolerance of E. marginata and E. calophylla than both E. diversicolor and E. patens to cold and dry surface-soil conditions. The ecological significance and practical implications of the results are discussed.

Notes: River and stream restoration projects are increasingly numerous but rarely subjected to systematic post-project evaluation. The few such evaluation studies conducted have indicated a high percentage of failures. Thus, post-project evaluation (and dissemination of results) is essential if the field of river restoration is to advance. Effective evaluation of project success should include: (1) Clear objectives, essential to identity potential incompatibilities among project objectives and to provide a framework for design of project evaluation. (2) Baseline data, needed as an objective basis for evaluating change caused by the project and encompassing as long a pre-project period as possible (including a detailed historical study). (3) Good study design, to demonstrate the effects of restoration projects in the complex riverine environment. (4) Commitment to the long term, to detect effects evident only years following project completion; in general, monitoring should continue for at least a decade, with surveys conducted after each flood above a predetermined threshold. (5) Willingness to acknowledge failures, or rather to recognize that each restoration project constitutes an experiment, so that a failure can be just as valuable to the science as a success, provided we can learn from it (which requires objective, robust post-project evaluation).

Notes: Festuca idahoensis (Idaho fescue) was a common native perennial bunchgrass in the sagebrush steppe of the western United States until the introductions of domestic livestock and alien plants. Restoration of Idaho fescue to degraded sites will likely involve reseeding, and one of the factors affecting reseeding success is germinability of the seeds employed. We investigated effects of after-ripening and storage temperature on germinability of Idaho fescue seeds collected from a central Oregon site. Six months of after-ripening were required before maximum germination was obtained. Storage of dry seeds at either room temperature (20°C) or at cooler, alternating temperatures (5/15°C) did not alter the rate at which dormancy was lost. Storage at the warmer temperature promoted rapid germination in seeds that had broken dormancy. Seed longevity varied greatly from year to year. Seeds produced in a very dry year had poorer germination and shorter longevity than seeds produced during a year with near normal precipitation. Because seed dispersal occurs in late July and early August for Idaho fescue in central Oregon, a six-month after-ripening requirement ensures that the greatest potential germination coincides with the spring period most likely to provide sufficient moisture for seedling establishment.

Notes: We conducted an experiment with the dual aims of (1) examining the feasibility of establishing a species-rich grassland using a commercially available grass and wild-flower seed mixture and (2) examining the effects of different defoliation and fertilizer managements on the productivity, species richness, diversity, and composition of a species-rich grassland established on a site reclaimed after opencast coal mining. The use of the seed mixture successfully established a sward of some 18–25 species per square meter. The species composition was enriched to some extent by recruitment of unsown species, principally from the soil seed bank. Hay-type defoliation management produced greater dry matter yield and species richness than grazing defoliation, but grazing defoliation produced greater species diversity. Fertilizer application had no significant effect on dry matter production but reduced species diversity. Ordination analysis revealed that both defoliation and fertilizer management significantly affected species composition. The response obtained by individual species was explicable largely by their comparative biology.

Notes: Books reviewed in this article: World Soil Erosion and Conservation. D. Pimentel, editor Environmental Impacts of Mining: Monitoring, Restoration and Control. M. Sengupta

Notes: We investigate why different states in the United States choose different regulatory plans in their telecommunications industry. We present a simple theoretical model and an empirical analysis of the issue. We find that a state is more likely to replace rate-of-return regulation with incentive regulation when: (1) residential basic local service rates have historically been relatively high; (2) allowed earnings under rate-of-return regulation in the state have been either particularly high or particularly low; (3) the state's leaders tend to come from both major political parties, rather than from a single party; (4) the state's urban population is growing relatively rapidly; and (5) the bypass activity of competitors in the state is less pronounced.

Notes: After the initial breakthrough in the research phase of R&D, a new product undergoes a process of change, improvement, and adaptation to market conditions. We model the strategic behavior of firms in this development phase. We emphasize that a key dimension to this competition is the innovation that leads to product differentiation and quality improvement. In a duopoly model with a single adoption choice, we derive endogenously the level and diversity of product innovations. We demonstrate the existence of equilibria in which one firm enters early with a low-quality product while the other continues to develop the technology and eventually markets a high-quality good. In such an equilibrium, no monopoly rent is dissipated and the later innovator makes more profits. Incumbent firms may well be the early innovators, contrary to the predictions of the “incumbency inertia” hypothesis.

Notes: This paper addresses the strategic commitment value of various price and nonprice vertical contracts. Assuming contract offers are sufficiently public to have strategic importance, we focus on commitment as a necessary and separable condition to be met. The analysis has two components. First, considering the relationship between a monopolist manufacturer and his imperfectly competing retailers, enforcement conditions necessary for alternative vertical contracts to have commitment value are identified in the static (reputation-free) context. It is shown that external help (i.e., enforcement by nonsignatories) is necessary for bilateral minimum resale price maintenance (price floors) and exclusive territory contracts to have commitment value, whereas maximum resale price maintenance (price ceilings) do not require such help (i.e., they are internally enforcing). Second, the paper provides an analysis of the U.S. case law and discusses the empirical validity of the “external enforcement” assumption that is shown to underlie recent proposals to make certain vertical restraints illegal.

Notes: Human exposure assessments require a linkage between toxicant concentrations in occupied spaces and the receptor's mobility pattern. Databases reporting distinct populations' mobility in various parts of the home, time outside the home, and time in another building are scarce. Temporal longitudinal trends in these mobility patterns for specific age and gender groups are nonexistent. This paper describes subgroup trends in the spatial and temporal mobility patterns within the home, outside the home, and in another building for 619 Iowa females that occupied the same home for at least 20 years. The study found that the mean time spent at home for the participants ranged from a low of 69.4% for the 50-59 year age group to a high of 81.6% for the over 80-year-old age group. Participants who lived in either one- or two- story homes with basements spent the majority of their residential occupancy on the first story. Trends across age varied for other subgroups by number of children, education, and urbadrural status. Since all of these trends were nonlinear, they indicate that error exists when assuming a constant, such as a 75% home occupancy factor, which has been advocated by some researchers and agencies. In addition, while aggregate data, such as presented in this report, are more helpll in deriving risk estimates for population subgroups, they cannot supplant good individual-level data for determining risks.

Notes: The National Research Council has recommended the use of an analytic/deliberative decision making process in environmental restoration decisions that involve multiple stakeholders. This work investigates the use of the results of risk assessment and multiattribute utility analysis (the “analysis”) in guiding the deliberation. These results include the ranking of proposed remedial action alternatives according to each stakeholder's preferences, as well as the identification of the major reasons for these rankings. The stakeholder preferences are over a number of performance measures that include the traditional risk assessment metrics, e.g., individual worker risk, as well as programmatic, cultural, and cost-related impacts. Based on these results, a number of proposals are prepared for consideration by the stakeholders during the deliberation. These proposals are the starting point for the formulation of actual recommendations by the group. In our case study, these recommendations included new remedial action alternatives that were created by the stakeholders after an extensive discussion of the detailed analytical results.

Notes: Comprehensive uncertainty analyses of complex models of environmental and biological systems are essential but often not feasible due to the computational resources they require. “Traditional” methods, such as standard Monte Carlo and Latin Hypercube Sampling, for propagating uncertainty and developing probability densities of model outputs, may in fact require performing a prohibitive number of model simulations. An alternative is offered, for a wide range of problems, by the computationally efficient “Stochastic Response Surface Methods (SRSMs)” for uncertainty propagation. These methods extend the classical response surface methodology to systems with stochastic inputs and outputs. This is accomplished by approximating both inputs and outputs of the uncertain system through stochastic series of “well behaved” standard random variables; the series expansions of the outputs contain unknown coefficients which are calculated by a method that uses the results of a limited number of model simulations. Two case studies are presented here involving (a) a physiologically-based pharmacokinetic (PBPK) model for perchloroethylene (PERC) for humans, and (b) an atmospheric photochemical model, the Reactive Plume Model (RPM-IV). The results obtained agree closely with those of traditional Monte Carlo and Latin Hypercube Sampling methods, while significantly reducing the required number of model simulations.

Notes: . Experimental evidence has been gathered to show that the life cycle of the myxozoan gallbladder parasite Zschokkella nova Klokacewa, 1914, which infects the fish Carassius carassius, has a complex life cycle with alternation of two hosts (fish and Oligochaeta) and two developmental phases (myxosporean and actinosporean). The gut epithelium of the oligochaete, Tubifex tubifex, exposed experimentally to Z. nova, obtained from C. carassius, became infected with organisms resembling Actinosporea. The spore structure and cube-like network of the interconnected spores is reminiscent of Siedleckiella silesica Janiszewska, 1952, although the spores are very different in size and number of sporoplasm nuclei. The life cycle of Z. nova resembles that of the whirling disease agent Myxosoma cerebralis described by Wolf and Markiw, which also alternates between fish and oligochaete hosts.

Notes: . Organisms in the phylum Apicomplexa appear to have a large extrachromosomal DNA which is unrelated to the mitochondrial DNA. Based on the apparent gene content of the large (35 kb) extrachromosomal DNA of Plasmodium falciparum, it has been suggested that it is a plastid-like DNA, which may be related to the plastid DNA of rhodophytes. However, phylogenetic analyses have been inconclusive. It has been suggested that this is due to the unusually high A + T content of the Plasmodium falciparum large extrachromosomal DNA. To further investigate the evolution of the apicomplexan large extrachromosomal DNA, the DNA sequence of the organellar ribosomal RNA gene from Toxoplasma gondii, was determined. The Toxoplasma gondii rDNA sequence was most similar to the large extrachromosomal rDNA of Plasmodium falciparum, but was much less A + T rich. Phylogenetic analyses were carried out using the LogDet transformation to minimize the impact of nucleotide bias. These studies support the evolutionary relatedness of the Toxoplasma gondii rDNA with the large extrachromosomal rDNA of Plasmodium falciparum and with the organellar rDNA of another parasite in the phylum Apicomplexa, Babesia bovis. These analyses also suggest that the apicomplexan large extra-chromosomal DNA may be more closely related to the plastid DNA of euglenoids than to those of rhodophytes.

Notes: . Seabottom sediments from a discontinued Philadelphia-Camden 40-Mile ocean sewage disposal site were cultured for cyst-forming free-living amoebae. Barge delivered wastes were discharged at the site from 1973 until 1980 when the site was closed. One station at the southeast margin of the site was sampled at a depth of approximately 50 m, twice in 1978 and once in 1982, 1983 and 1984. Sediment from the 1978 collection yielded Acanthamoeba polyphaga, Vahlkampfia sp., and an unknown amoeba with stellate endocysts similar to those of A. astronyxis. Trophozoites and cysts of the isolate were typical of those described for the genus Acanthamoeba. Biochemical tests employing enzyme electrophoresis and morphological studies on live and stained specimens showed that the isolate was distinct from other well-described species within the family Acanthamoebidae Sawyer & Griffin, 1975.

Notes: . Alexandrium tamarense (Lebour) Balech (=Gonyaulax tamarensis Lebour) has been widely distributed and occasionally abundant in coastal waters of Long Island, New York in recent years. However, the distribution on the New Jersey coast has been sparse and this fact cannot be explained by this dinoflagellate's ability to migrate, or by its nutritional and physiological characteristics, or by the region's general suitability for phytoplankton. Therefore, the possibility that New Jersey coastal waters might be chemically exclusionary for A. tamarense seemed worth exploring. In a limited approach, we tested for water quality detrimental to the species in one New Jersey site (Great Bay) with a series of annual assays. Parsonage Creek, Long Island, New York, was assayed for comparison; this creek is assumed to have had at least marginal suitablity for A. tamarense, based on its reported long-term presence. Results provide tentative support for our working hypothesis, i.e. Great Bay chemical water quality is generally unfavorable for A. tamarense. Inhibition of A. tamarense growth, or culture decline, occurred in both assay series, but was substantially greater in Great Bay water. Inimical water quality was the most important factor distinguishing the two sites. Chelation with EDTA had greatest overall benefit in Great Bay assays, suggesting that lower availability of a natural chelator in the bay could be a secondary factor. Assay metal response is problematic, but we believe it permits speculation that essential metals could be partially limiting to A. tamarense in Great Bay, but would not be a critical regulator.

Notes: . Experimental transmission of two bacterial endosymbionts to symbiont-free isolates of Acanthamoeba spp. was studied to determine specificity of the host-symbiont relationship. Both symbionts originated from amoebic isolates displaying an identical mitochondrial DNA EcoRI fingerprint (group AcUW II). Symbioses were readily established in one amoebic isolate which displayed a homologous mtDNA fingerprint (group AcUW II). Exposure of a heterologous amoebic isolate (group AcUW IV) to the two symbionts resulted in either cell death or encystation without the establishment of symbioses. While symbioses were established with an amoebic isolate from a second heterologous group (AcUW I), a unique membranous sheath appeared and persisted around one of the symbionts which did not exist in the original host. An isolate representing a third heterologous amoebic group (AcUW VI) was variable in its susceptibility with one symbiont unable to infect the host and the other becoming established only after an initial reaction in which trophozoites rounded-up and floated off the substrate. These studies suggest that a specific recognition system exists between particular isolates of Acanthamoeba and their symbionts, and that the appearance of a killer phenotype is related to contact between mismatched, though recognized, pairs.

Notes: . Microsporidian spores were developed from cells which were grown in vitro from a human liver lesion which was due to larval Echinococcus multilocularis. The microsporidian spores developed in the same fashion as an Encephalitozoon cuniculi. The Encephalitozoon-like spores were completely separated on Percoll gradients. The separated spores contained DNA capable of amplification by two different primer sets designed for the polymerase chain reaction (PCR) of E. multilocularis DNA. However, the cell DNA from which microsporidium developed was thoroughly insensitive to the PCR using the E. multilocularis primer sets. The results strongly suggested that Encephalitozoon should be taken into consideration, when DNA isolated from larval E. multilocularis is analyzed.

Notes: . We have used the anti-phosphoprotein antibody MPM-2 to examine changes in phosphorytation of cortical proteins during cilia regeneration in Tetrahymena thermophila. Although numerous cortical proteins are phosphorylated in both nondeciliated and deciliated cells, deciliation induces a dramatic increase in the phosphorylation of a 90-kDa cortical protein. The 90-kDa protein remained phosphorylated during cilia regeneration and then gradually became dephosphorylated. The 90-kDa protein was phosphorylated and dephosphorylated normally in Tetrahymena mutants that assemble short cilia, suggesting that achievement of full length is not the signal that triggers dephosphorylation of the 90-kDa protein. When initiation of cilia assembly is blocked, the 90-kDa protein becomes phosphorylated and remains phosphorylated for an extended period of time, suggesting that initiation of cilia elongation triggers eventual dephosphorylation of the 90-kDa protein, regardless of how long the cilia actually become.

Notes: . Giardia lamblia trophozoites express on their surfaces one of a set of cysteine-rich antigenically variant proteins, called variant-specific surface proteins, which comprise the majority of proteins detected by surface labeling. While these VSP proteins may be immunodominant proteins important in the host immune response to G. lamblia, the ability to switch expression from one VSP to another may provide a means for the trophozoites to avoid the host immune response. The first VSP characterized, VSPA6 (from the A6 clone of the WB isolate, originally termed CRP170), contains 18–23 copies of a 65 amino acid repeat. We have now used the repeat as a probe to isolate from a WBA6 genomic library two genes related to vspA6 (called vspA6-S1, vspA6-S2). Sequence analysis of the vspA6-S1 gene revealed nearly two complete copies of the 195 bp repeat and substantial nucleotide and translated amino acid similarity in the coding regions 5’and 3’to the repeats. The vspA6-S2 gene, while still related, showed greater divergence from vspA6 than vspA6-S1 in the nonrepeat coding region and contained nearly four copies of a 201 bp repeat that was 75% identical to the 195 bp vspA6 repeat. These results suggest that gene duplication followed by divergence has played a key role in the generation of the vsp gene repertoire.

Notes: . The glycerophosphate oxidase (GPO), the unique terminal oxidase of bloodstream trypanosome (TAO), appears to be functionally similar to the alternative oxidases of some plants and higher fungi. Immunoblotting of mitochondrial proteins of bloodstream trypomastigotes of Trypanosoma brucei brucei with monoclonal or polyclonal antibodies to Sauromatum guttatum (voodoo lily) and Symplocarpus foetidus (skunk cabbage) alternative oxidases respectively revealed two proteins of about 33 kDa (p33) and 68 kDa (p68). These proteins are not present in procyclic trypomastigotes. Electrophoresis under rigorous denaturing conditions indicated p68 to be the dimer of p33. Indirect immunofluorescent studies of bloodstream and procyclic trypomastigotes with monoclonal antibody to plant alternative oxidase also showed the localization of 33 kDa protein in the mitochondria of the bloodstream trypomastigotes. The functional TAO activity could be solubilized efficiently from the mitochondrial membrane of the bloodstream trypomastigotes by 1% NP-40 or 10 mM lauryl maltoside. When fractionated by Superose 12 gel filtration chromatography, p33 was co-purified with the TAO enzymatic activity. The apparent molecular size of the active enzyme complex was found to be 160 kDa. Gradual disappearance of the 33 kDa protein and the TAO enzymatic activity were well correlated during in vitro differentiation of the bloodstream to procyclic trypomastigotes. This study implies that the net biosynthesis of p33, an essential subunit of TAO, is decreased during differentiation from bloodstream to procyclic trypomastigotes.

Notes: . The microsporidium, Enterocytozoon bieneusi, causes a severe, debilitating, chronic diarrhea in patients with the acquired immunodeficiency syndrome. Specific diagnosis of intestinal microsporidiosis, especially due to Enterocytozoon, is difficult and there is no known therapy that can completely eradicate this parasite. Preliminary studies indicate that a short term (about 6 months) in vitro culture of this parasite yielding low numbers of spores, may be established by inoculating human lung fibroblasts and/or monkey kidney cell cultures with duodenal aspirates and or biopsy from infected patients. The cultures may subsequently be used for the isolation and molecular analysis of parasite DNA.

Notes: . A new Myxosporea, Ceratomyxa sparusaurati n. sp., was found in the gall bladder and bile of cultured gilthead sea bream (Sparus aurata L.) from different Spanish fish farms. It is clearly different from all the previously reported Ceratomyxa from sparids, and it is distinguished from other members of the genus by the shape and size of the spores. Prevalence of infection was 2.15% in an Atlantic farm, 48.7% in a Mediterranean farm and 28.6% in the facilities of the Instituto de Acuicultura de Torre de la Sal. The cell-in-a-cell pattern was found through all the sporogenesis and the general ultrastructure resembled other Myxosporea. Primary cells with two developing spores, harbored other secondary cells. Disporous sporoblasts contained numerous membrane-bound inclusions, a few lipid droplets and polysaccharides as evidenced by cytochemistry. In mature spores, binucleate sporoplasmic cells contained abundant Thièry-negative sporoplasmosomes.

Notes: . The immobilization antigens (i-antigens) are a class of highly abundant surface membrane proteins found on a number of holotrich ciliates. In Ichthyophthirius multifiliis (an obligate parasite of fish) these antigens appear to be targets of the host immune response. While the i-antigens of Ichthyophthirius are predominantly membrane-associated proteins, we now find that they are released into the water surrounding the parasite in a highly enriched form. The membrane-associated and water soluble proteins appear indistinguishable by antigenic means, as well as by several biochemical criteria including peptide mapping, mobility in reducing and non-reducing SDS-polyacrylamide gels, and relative glycosylation. Antibodies raised against the membrane-associated antigens react with the water soluble proteins on Western blots. Not surprisingly, immunocytochemical localization studies show binding of these antibodies to surface membranes of the cell. In addition, however, antibody binding is also detectible on the membranes of a secretory organelle (that is, mucocysts) present in the cortical cytoplasm. The significance of these findings with regard to the potential role of the i-antigens in infection and immunity is discussed.

Notes: . The complete nucleotide sequence has been established for two genes (maeA and maeB) coding for different subunits of the hydrogenosomal malic enzyme [malate dehydrogenase (decarboxylating) EC 1.1.1.39] of Trichomonas vaginalis. Two further genes (maeC and maeD) of this enzyme have been partially sequenced. The complete open reading frames code for polypeptides of 567 amino acids in length. These two open reading frames are similar with less than 12 percent pairwise nucleotide differences and less than 9 percent pairwise amino acid differences. The open reading frames of the two partially sequenced genes correspond to the amino-terminal part of the polypeptides coded and are similar to the corresponding parts of the completely sequenced ones. The deduced translation products of the two complete genes differ in their calculated pI values by 1.5 pH unit. The genes code for polypeptides which contain 12 or 11 amino-terminal amino-acyl residues not present in the proteins isolated from the cell. Other hydrogenosomal enzymes also have similar amino-terminal extensions which probably play a role in organellar targeting and translocation of the newly synthesized polypeptides. A comparison of 19 related enzymes from bacteria and eukaryotes with the maeA product revealed 34–45 percent amino acid identity. Phylogenetic reconstruction based on nonconservative amino acid differences with maximum parsimony (phylogenetic analysis using parsimony, PAUP) and distance based (neighbor-joining, NJ) methods showed that the T. vaginalis enzyme is the most divergent of all eukaryotic malic enzymes, indicating its long independent evolutionary history.