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  • Coleoptera  (136)
  • Springer  (136)
  • Institute of Physics
  • 1995-1999  (34)
  • 1990-1994  (102)
  • 1940-1944
  • 1996  (34)
  • 1994  (58)
  • 1993  (44)
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  • 1995-1999  (34)
  • 1990-1994  (102)
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
    Abiotic mortality factors of the coffee berry borer (Hypothenemus hampei) (1994)
    Springer
    Entomologia experimentalis et applicata 71 (1994), S. 201-209 
    Details
    ISSN: 1570-7458
    Keywords: Hypothenemus hampei ; coffee berry borer ; abiotic mortality ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mortality of the coffee berry borer was studied under controlled laboratory conditions in Tapachula, Mexico. For adult female borers subjected to a range of relative humidities (RH) without food at 25°C, the longest mean survival time (20 days) was obtained at 93.5% RH. Adult borer survival was also studied at a range of temperatures for a fixed relative humidity (93.5% RH); at 20°C mean survival time was 28 days. Fecundity and mortality of borer stages in berries was studied for a range of humidities at 25°C. Maximum fecundity was obtained at 90 and 93.5% RH. Immature stages were ejected from the berry at 84% RH and above, which is interpreted as a form of brood hygiene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426404
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  • 2
    Electronic Resource
    Electronic Resource
    Field trapping of Metamasius hemipterus with synthetic aggregation pheromone (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 453-460 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Curculionidae ; Metamasius hemipterus ; aggregation pheromone ; redundancy ; field trapping ; sex-ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The five synthetic pheromone components of the West Indian sugarcane borer (WISB), Metamasius hemipterus (L.) (Coleoptera, Curculionidae) were tested in the field. The combination of sugarcane (SC) and the major pheromone compound, 4-methyl-5-nonanol (1) was attractive. However, the addition of 2-methyl-4-heptanol (2) or 2-methyl-4-octanol (3) was required to reach high catch levels while 5-nonanol (4) or 3-hydroxy-4-methyl-5-nonanone (5) did not enhance WISB attraction. The redundancy phenomenon, here reported for the first time in rhynchophorinous species, was observed between compounds 2 and 3. SC +1 +3 was more attractive than living male baits, however, the sex-ratio of the catches was equivalent between both treatments. The sex-ratio of catches was affected by the qualitative composition of the pheromone formulation. Compound 3 had a sexual role, attracting more females while 5 seemed to play an aggregation role, luring both sexes in the same proportion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343468
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  • 3
    Electronic Resource
    Electronic Resource
    Behavioral responses of Colorado potato beetle larvae to combinations of temperature and insolation, under field conditions (1994)
    Springer
    Entomologia experimentalis et applicata 72 (1994), S. 255-263 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Chrysomelidae ; Leptinotarsa decemlineata ; behavior ; thermal biology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In short-term field trials at combinations of ambient temperature (°C) and insolation (W·m−2), larval Colorado potato beetles (Leptinotarsa decemlineata [Say] [Coleoptera: Chrysomelidae]) were observed after their release on the adaxial surface of leaflets on potato plants (Solanum tuberosum L. Solanaceae). The larvae either began feeding or moved under the leaflet; mean interval from release to expression of these behaviors (2.9±0.05 min [n =358]) was independent of air temperature and insolation. Proportion of larvae moving under the leaflet increased logistically with both air temperature and insolation. A 1 W·m−2 change in insolation (P) evoked the same effect on this proportion as a 0.0838 °C change in air temperature (T a ), so the two quantities were combined as T*=T a +P·0.0838 °C/(W·m−2), which has units of °C. The proportion of larvae moving under the leaflet increased logistically with T*. In 1-day field trials we monitored air temperature, insolation and proportion of larvae under the leaflet, and compared the latter to predictions from the logistic regression derived from the short-term trials. Consistently more larvae occurred under leaflets than predicted from the logistic regression; this bias diminished as T* increased until at T*≥40 °C, observed and predicted proportions were equal. This pattern of deviation from the predictions of the logistic regression is consistent with a thermoregulatory strategy in which larvae move away from hostile conditions, rather than seek optimal conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02383813
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  • 4
    Electronic Resource
    Electronic Resource
    Mortality in Oryzaephilus surinamensis following short-term exposure to conditioned kernels by high-density culture (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 555-557 
    Details
    ISSN: 1570-7458
    Keywords: Oryzaephilus surinamensis ; Coleoptera ; Cucijidae ; population density ; semiochemicals ; lethal secretions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343481
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  • 5
    Electronic Resource
    Electronic Resource
    Evaluation of artificial diets and two species of natural prey as laboratory food forChilocorus spp. (1993)
    Springer
    Entomologia experimentalis et applicata 69 (1993), S. 13-20 
    Details
    ISSN: 1570-7458
    Keywords: Artificial diets ; Coccinellidae ; rearing ; biological control ; Chilocorus spp. ; Coleoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Promising diets were screened and the most successful modified with additives used in artificial diets for other entomophagous insects. Two suitable diets were obtained, one for adults and one for larvae ofChilocorus nigritus (Fabricius) (Coleoptera: Coccinellidae). They were still inferior to natural prey and not adequate as the sole food source for rearing consecutive generations. They are valuable as substitute food in the insectary during shortages of natural prey. Oleander scaleAspidiotus nerii Bouché andAsterolecanium miliaris (Boisduval) were evaluated as natural prey forC. nigritus and two other potential biocontrol agents in southern Africa,C. bipustulatus (Linnaeus) andC. infernalis Mulsant.A. nerii andA. miliaris were suitable for all life stages ofC. nigritus and adults ofC. bipustulatus andC. infernalis. A. miliaris was inadequate for larvae ofC. bipustulatus andC. infernalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380669
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  • 6
    Electronic Resource
    Electronic Resource
    Avoidance of willows from moderately polluted area by leaf beetle, Melasoma lapponica: effects of emission or induced resistance? (1996)
    Springer
    Entomologia experimentalis et applicata 79 (1996), S. 355-362 
    Details
    ISSN: 1570-7458
    Keywords: aerial pollution ; heavy metals ; sulphur dioxide ; feeding preference ; Salix ; Melasoma lapponica ; Coleoptera ; Chrysomelidae ; population density ; induced resistance ; plant communication ; Kola Peninsula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied preference for willows along a pollution gradient on the Kola Peninsula, Russia, by the leaf beetle, Melasoma lapponica. Multiple tests with leaf disks demonstrated low preference for Salix borealis, S. caprea and S. phylicifolia from the plot situated 14 km from the smelter, in comparison with conspecific plants from plots situated at 1 and 29 km distances. This pattern was observed when testing beetles orginating from any plot both in 1993 and 1994, using both young and mature leaves of S. borealis. Although fumigation of S. borealis with realistic SO2 concentration (100 μg/m3) increased plant palatability, preference for plants from our study plots did not correlate with plot-specific mean SO2 concentrations. Furthermore, no correlation with foliar concentrations of the main metal pollutants (Ni and Cu) was found. Palatability of plants was negatively correlated with population density of M. lapponica, which peaked in the moderately polluted plot 14 km from the smelter. Within this plot, beetles clearly preferred non-damaged bushes of S. borealis to previously damaged bushes. We therefore conclude that low preference of S. borealis from the moderately polluted area was caused by plant resistance induced by severe damage from M. lapponica in previous years rather than by pollution impact. However, S. caprea and S. phylicifolia had little damage from M. lapponica, and low palatability of these species in the moderately polluted plot suggests changes in plant quality similar to changes in heavily damaged bushes of S. borealis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186294
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  • 7
    Electronic Resource
    Electronic Resource
    Examination of anAspergillus flavus resistant inbred of maize for crossresistance to sap beetle vectors (1994)
    Springer
    Entomologia experimentalis et applicata 71 (1994), S. 177-180 
    Details
    ISSN: 1570-7458
    Keywords: aflatoxin ; Carophilus ; Zea mays ; corn ; plant resistance ; Coleoptera ; Nitidulidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382385
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  • 8
    Electronic Resource
    Electronic Resource
    Intraspecific variability in adult flea beetle behaviour and larval performance on an atypical host plant (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 160-162 
    Details
    ISSN: 1570-7458
    Keywords: Phyllotreta nemorum ; Barbarea vulgaris ; Coleoptera ; Chrysomelidae ; Cruciferae ; resistance ; genetics of insects ; inheritance ; Y-linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194748
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  • 9
    Electronic Resource
    Electronic Resource
    Glandular trichomes ofSolanum berthaultii alter host preference of the Colorado potato Beetle,Leptinotarsa decemlineata (1994)
    Springer
    Entomologia experimentalis et applicata 70 (1994), S. 217-225 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Chrysomelidae ; Leptinotarsa decemlineata ; Colorado potato beetle ; Solanaceae ; Solanum berthaultii ; plant resistance ; glandular trichomes ; host preference ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Choice and no-choice studies were conducted to determine how the glandular trichomes of the wild potato,Solanum berthaultii Hawkes, affect host preference of the Colorado potato beetle,Leptinotarsa decemlineata (Say). Given a feeding choice betweenS. tuberosum andS. berthaultii, larvae and adults preferred the foliage ofS. tuberosum, but adults were more discriminating. When foliage ofS. berthaultii was appressed toS. tuberosum leaflets, fewer adults fed on the appressed leaflets. When given a choice between ‘trichome-intact’ and ‘trichome-removed’S. berthaultii foliage, adults preferred to feed on the latter. The preference for ‘trichome-removed’ foliage and the percent of adults initiating feeding, increased with the degree of trichome removal. These studies provide evidence that the resistance ofS. berthaultii is associated with feeding deterrents localized in the glandular trichomes, thatS. berthaultii possesses more than one mechanism of resistance to the Colorado potato beetle, and that the expression of resistance is dependent on the developmental stage of the insect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380555
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  • 10
    Electronic Resource
    Electronic Resource
    The behaviour of 9 stored product beetles at pitfall trap arenas and their capture in millet (1993)
    Springer
    Entomologia experimentalis et applicata 66 (1993), S. 161-169 
    Details
    ISSN: 1570-7458
    Keywords: avoidance behaviour ; capture efficiency ; Coleoptera ; time-lapse video ; visual recording
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pitfall trap capture forSitophilus oryzae (L.),S. zeamais (Motschulsky),S. granarius (L.),Tribolium confusum (Duval),T. castaneum (Herbst);Oryzaephilus surinamensis (L.),Cryptolestes pusillus (Schonherr),Rhyzopertha dominica (Fabr.), andProstephanus truncatus (Horn) in millet was assessed by visual and time-lapse video recordings. The behaviour of different beetle species in arenas containing millet was monitored over 24 h and the frequency of encounters with the trap rim resulting in capture recorded. The capture efficiency of four types of pitfall traps (i.e. polystyrene, polythene, glass and tin-plated steel can) with rims exposed or submerged below the millet surface level were compared. Capture was related to beetle size, locomotory rate, and beetle behaviour at the trap rim as well as trap design and placement. The lighter and smaller species were least captured. Glass jars were more effective than plastic and metal containers. Traps placed with their rims submerged below the grain surface level were more efficient than those with rims exposed. Capture rate was unrelated to trap size. The frequency of encounters with trap rims was not correlated with capture rate. Three types of avoidance behaviours at the trap rims i.e. probing, skirting and spontaneous retreat, were related to capture rate, spontaneous retreat being the most effective escape mechanism and probing least.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382285
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  • 11
    Electronic Resource
    Electronic Resource
    Distribution of overwintering Colorado potato beetle in and near Massachusetts potato fields (1993)
    Springer
    Entomologia experimentalis et applicata 66 (1993), S. 191-196 
    Details
    ISSN: 1570-7458
    Keywords: Leptinotarsa decemlineata (Say) ; Coleoptera ; Chrysomelidae ; distribution ; diapause ; overwintering ; mortality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382289
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  • 12
    Electronic Resource
    Electronic Resource
    The role of symbiotes in flight activity ofSitophilus weevils (1994)
    Springer
    Entomologia experimentalis et applicata 70 (1994), S. 201-208 
    Details
    ISSN: 1570-7458
    Keywords: Sitophilus oryzae ; S. zeamais ; Coleoptera ; Curculionidae ; flight activity ; symbiosis ; vitamin ; amino acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les performances de vol de nombreuses souches deSitophilus oryzae L. etS. zeamais Mots. (Coleoptera, Curculionidae) ont été comparées sur blé. Il s’avère que les mâles sont plus actifs que les femelles et queS. zeamais possède une meilleure aptitude au vol queS. oryzae. De plus, dans les deux espèces élevées sur blé, seuls les charançons normalement symbiotiques sont capables de voler, tandis que les aposymbiotiques ont perdu ce pouvoir. En complémentant la nourriture avec des vitamines (acide pantothénique et riboflavine) et certains acides aminés (phénylalanine et proline), cette activité a pu être restaurée en partie. L’influence de la symbiose sur l’aptitude au vol du charançon du genreSitophilus est commentée en fonction du métabolisme énergétique (coenzymes, proline) et du rôle joué par la symbiose dans ce comportement qui permet d’augmenter la dissémination de l’espèce.
    Notes: Abstract The flight activity (take-off) of various strains ofSitophilus oryzae L. andS. zeamais Mots. (Coleoptera, Curculionidae) have been compared on wheat. It was shown that males are more active than females and thatS. zeamais, is more active thanS. oryzae. Furthermore, in the two species, when reared on wheat kernels, only symbiotic weevils were able to fly, while aposymbiotic insects did not. By supplementation of the diet with vitamins (pantothenic acid, riboflavin) and amino acids (phenylalanine and proline), this activity could be restored in part. The influence of symbiosis on the flight ability ofSitophilus weevil is discussed in relation to the energy metabolism (coenzymes, proline), the role played by symbiosis in this behaviour and its possible effect on the dissemination of the species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380553
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  • 13
    Electronic Resource
    Electronic Resource
    Physiology and costs of resistance to herbivory and disease in Brassica (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 231-237 
    Details
    ISSN: 1570-7458
    Keywords: Leptosphaeria maculans ; Peronospora parasitica ; fungal pathogens ; Phyllotreta cruciferae ; flea beetles ; Coleoptera ; Chrysomelidae ; Cruciferae ; chitinase ; myrosinase ; allocation costs ; plant defense mechanisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used artificial selection experiments to study genetic allocation costs and physiological mechanisms of resistance to herbivory and fungal disease. Genetic costs to resistance were present in some instances and absent in others. Genetic resistance to the fungal pathogen, Leptosphaeria maculans was cost-free, while resistance to Peronospora parasitica showed a negative genetic correlation between disease resistance and growth rate. Leptosphaeria resistant genotypes had 13% higher chitinase activity. Genetic increases in myrosinase activity were correlated with increased resistance to flea beetles (Phyllotreta cruciferae), but resulted in lower plant fecundity, presumably due to production costs of myrosinase. Genetic costs of resistance may maintain genetic variation in natural plant populations. These studies demonstrate the predictive and explanatory power of a functional approach to plant-herbivore and plant-pathogen interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194764
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  • 14
    Electronic Resource
    Electronic Resource
    Tritrophic interactions among larval parasitoids, bruchids and Leguminosae seeds; influence of pre-and post-emergence learning on parasitoids' response to host and host-plant cues (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 293-296 
    Details
    ISSN: 1570-7458
    Keywords: ectoparasitoids ; semiochemicals ; parasite host-selection ; Eupelmus vuilleti ; Hymenoptera ; Eupelmidae ; Dinarmus basalis ; Pteromalidae ; Coleoptera ; Bruchidae ; Bruchidius atrolineatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194778
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  • 15
    Electronic Resource
    Electronic Resource
    Geographic variation in feeding and mating preferences in the Phratora tibialis complex (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 311-314 
    Details
    ISSN: 1570-7458
    Keywords: leaf beetles ; Coleoptera ; Chrysomelidae ; feeding preference ; mating ; willows ; Salix
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194781
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  • 16
    Electronic Resource
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    Effects of host plant and cucurbitacin on growth of larval Diabrotica undecimpunctata howardi (1996)
    Springer
    Entomologia experimentalis et applicata 81 (1996), S. 47-51 
    Details
    ISSN: 1570-7458
    Keywords: southern corn rootworm ; plant-insect interaction ; plant chemistry ; Coleoptera ; Chrysomelidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of host plant and dietary cucurbitacin on the growth of larval southern corn rootworm (SCR), Diabrotica undecimpunctata howardi Barber (Chrysomelidae: Luperini), were investigated. SCR were reared on four hosts: corn, Zea mays; peanuts, Arachis hypogaea; and two squash varieties, Cucurbita pepo cv. Ambassador (containing cucurbitacin D (0.08 mg g−1 fr.wt.) = bitter), and C. pepo cv. Early Yellow Crookneck (lacking cucurbitacin = non-bitter). Larval growth was significantly greater on corn and peanuts than on either squash variety. After four weeks, adults had emerged from corn and peanut plants, while squash-reared larvae had not yet entered the pupal stage. There was no difference in larval growth on the two varieties of squash. Primary metabolite measurements showed no nutritional differences between the two squash varieties. Artificial diet experiments were used to test the effect of three concentrations of cucurbitacin D (0.0, 0.1, and 0.6 mg g−1 diet) on growth of larval SCR. Larvae reared on diet containing 0.6 mg g−1 cucurbitacin weighed significantly less than larvae reared on diet containing 0.1 mg g−1 or no cucurbitacin after 10 d. No significant difference in growth was measured between the 0.1 mg g−1 diet and the 0.0 mg g−1 diet. Results are discussed relative to theories about the relationship between diabroticites and cucurbitacins.
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    URL: http://dx.doi.org/10.1007/BF00187837
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  • 17
    Electronic Resource
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    Disrupting spring colonization of Colorado potato beetle to nonrotated potato fields (1994)
    Springer
    Entomologia experimentalis et applicata 73 (1994), S. 39-50 
    Details
    ISSN: 1570-7458
    Keywords: Leptinotarsa decemlineata ; Coleoptera ; Chrysomelidae ; distribution ; overwintering ; diapause ; cultural control ; trap cropping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Overwintering Colorado potato beetles (Leptinotarsa decemlineata (Say)) were concentrated primarily within woody borders, and mortality was lower in borders than in potato fields. After overwintering, only 15–44% of live beetles were in the potato fields. In experiments with small plots, colonization of fields from woody borders was reduced ∼60% by a trap crop, either treated with adulticide or with beetles collected daily. Such trap crops, or simply pitfall traps to prevent colonization from woody borders, could significantly reduce early-season adult numbers and subsequent larval populations. However, success is dependent on the local densities of overwintered beetles, and the prevalence of arrestment behavior in the case of trap crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382512
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  • 18
    Electronic Resource
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    Selection response of the Colorado potato beetle for adaptation to the resistant potato,Solanum berthaultii (1994)
    Springer
    Entomologia experimentalis et applicata 73 (1994), S. 101-109 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Chrysomelidae ; Leptinotarsa decemlineata ; Colorado potato beetle ; Solanaceae ; Solanum berthaultii ; potato ; plant resistance ; selection ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thirty cohorts of the Colorado potato beetle (CPB) were reared for ten consecutive generations on the cultivated potato,Solanum tuberosum, and on two accessions (PI 473331 and PI 473334) of the resistant wild species,S. berthaultii. At each generation, selection was imposed for increased survival, decreased development time, and for increased fecundity. Although several parameters responded to selection, net replacement rate (R0) onS. berthaultii was consistently less than 1/3 that on cultivated potato. At the tenth generation, eachS. tuberosum strain female replaced herself with 110 daughters, whereas females from strains reared on PI 473334 and PI 473331 produced an average of 30 and 7 daughters, respectively. Slopes of the R0 regression lines for populations reared onS. tuberosum andS. berthaultii did not differ from zero, indicating no significant response to selection. Our findings suggest that populations of the Colorado potato beetle may have genetic variability in some performance traits for adaptation toS. berthaultii, but that adaptation is unlikely to occur as rapidly as previously reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02383415
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  • 19
    Electronic Resource
    Electronic Resource
    Spring behavioral patterns of the apple blossom weevil (1996)
    Springer
    Entomologia experimentalis et applicata 79 (1996), S. 9-17 
    Details
    ISSN: 1570-7458
    Keywords: Anthonomus pomorum ; temperature ; diel cycle ; Coleoptera ; Curculionidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Individual pairs of overwintered adult apple blossom weevils, Anthonomus pomorum (L.), confined with apple twigs under different ambient temperatures in the laboratory and on apple trees in the field, were observed through day and night for their spring activities. Flight behavior in relation to ambient temperature was also investigated under laboratory conditions using flight stands. Both sexes displayed predominantly nocturnal behavior patterns in both the laboratory and the field. Feeding, crawling, and mating activities increased following sunset in the field or onset of scotophase in the laboratory while resting occurred most frequently during daylight hours. Results of the laboratory experiments showed that temperature affected significantly the activity patterns. The diel pattern of activities became less distinctive at higher temperatures (above 15°C), and total activities in crawling, feeding, and mating were suppressed significantly at lower temperatures (below 5°C). Over 97% of the test weevils initiated take-off response from flight stands at 20°C within the 30 min trial period; however, flight initiation rarely occurred at temperatures 12°C or below. Overall, results of the laboratory and field experiments indicate that A. pomorum is a remarkably cold-adapted insect with ability to crawl, feed, and mate at a few degrees above freezing, a physiological attribute necessary for the exploitation of early stages of apple bud development in the cold early spring.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333816
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  • 20
    Electronic Resource
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    Snowdrop and wheatgerm lectins and avidin as antimetabolites for the control of sugarcane whitegrubs (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 409-414 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Scarabaeidae ; Antitrogus consanguineus ; Antitrogus parvulus ; avidin ; snowdrop lectin ; wheatgerm lectin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Snowdrop and wheatgerm lectins were found to be insecticidal and growth inhibiting dietary proteins for larvae of the sugarcane whitegrub Antitrogus parvulus. At concentrations as low as 0.5 mg of snowdrop lectin per gram of semi-artificial diet, growth was inhibited by 21 days of feeding and significant mortality was apparent by 28 days. Wheatgerm lectin was active at similar concentrations, although expression of the effects was slower. Avidin was found to be a growth inhibiting dietary protein for larvae of Antitrogus consanguineus. At levels as low as 0.01 mg g-1 of diet, growth was inhibited by 28 days of feeding. Avidin caused no significant mortality after 35 days of feeding. Snowdrop and wheatgerm lectins and avidin are insect growth-inhibiting proteins whose genes potentially could be manipulated into sugarcane and improve host-plant resistance to whitegrubs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00188033
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    Flash behavior of femalePhoturis versicolor fireflies (Coleoptera: Lampyridae) in simulated courtship and predatory dialogues (1994)
    Springer
    Journal of insect behavior 7 (1994), S. 843-858 
    Details
    ISSN: 1572-8889
    Keywords: firefly ; entrainment ; insect communication ; Coleoptera ; aggressive mimicry ; bioluminescence ; Lampyridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract FemalePhoturis versicolor fireflies attempt to capture males by responding to heterospecific flash patterns. A mating-dependent switch occurs which affects response timing and frequency of female flashes. We examined the switch using females of known age, mating status, and flash experience to assess how accurate mimicry is, what factors influence it, and what mechanism produces it. Presentations of simulated male flash patterns before and after mating revealed elements of an entrainment mechanism controlling female responsiveness. Unmated females preferentially answered conspecific patterns with variable latencies, averaging 1 s. Mating induced changes in both response frequency and response latency: Females answered heterospecific patterns more frequently, and latencies elicited by conspecific patterns shifted away from the unmated range. Heterogeneity in mean and variance of response latency among individuals indicates that females do not share a discrete reply to a given pattern. Little correspondence exists between latencies of sympatric species andP. versicolor females, suggesting that the flash response mechanism produces entriainment to any rhythmic pattern, not a one-to-one matching between prey and predator latencies. Different selective scenarios underlie strict mimicry versus entrainment mimicry.
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    URL: http://dx.doi.org/10.1007/BF01997130
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    Flight activity ofProstephanus truncatus (Horn) (Coleoptera: Bostrichidae) in relation to population density, resource quality, age, and sex (1996)
    Springer
    Journal of insect behavior 9 (1996), S. 339-351 
    Details
    ISSN: 1572-8889
    Keywords: Prostephanus truncatus ; larger grain borer ; Coleoptera ; Bostrichidae ; flight ; stored product insect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Higher flight activity has been observed in aged, high-density cultures ofProstephanus truncatus (Horn) (Coleoptera: Bostrichidae), but adults in new, lowdensity culture jars showed less flight activity. In order to understand this change in behavior, the effects of population density, age, resource quality, and sex on the flight ofP. truncatus were studied in a wind tunnel. While an immediate density on the release platform had no significant effect on flight, beetles from high-density cultures were more inclined to fly than those from low-density cultures. Resource quality exerted a major influence on flight; insects in food suitable for boring and oviposition seldomly exhibited flight, however, when food was absent or of inferior quality for boring and oviposition, the dominant behavior was flight. Also, insects maintained for a week in food suitable for boring and oviposition were less ready to fly than those maintained in food unsuitable for boring and oviposition. The optimum age range for flight activity was before the peak of reproduction and insects rarely flew before 4 days or after 32 days of emergence. There were no significant differences between the flight activity of males and that of females. Based on these results, we conclude that age and resource quality are major influences on the flight activity ofP. truncatus and a hypothesis is proposed in which reproductively active male and female beetles disperse from habitats of low resource quality to those that support their reproductive behavior. The practical implications of these results and the possible role of the male-produced aggregation pheromone are discussed.
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    URL: http://dx.doi.org/10.1007/BF02213876
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    Behavioural responses of three stored product Coleoptera species to extract of carob (Locust bean),Ceratonia siliqua (1993)
    Springer
    Entomologia experimentalis et applicata 68 (1993), S. 9-13 
    Details
    ISSN: 1570-7458
    Keywords: bioassay ; behavioural response ; Coleoptera ; carob extract ; stored product
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The behavioural responses ofCryptolestes pusillus Oryzaephilus surinamensis andProstephanus truncatus to different doses of carob extract were assessed in a two-choice pitfall bioassay and in plastic grain probe traps. The extract evoked a quick directional response and induced high beetle attraction to treated pitfall arenas compared with the controls. Overall, the extract improved probe trap efficiency by about 50% compared to unbaited probes. Beetle response was dose-dependent with the medium dose of 10 μl probably eliciting optimum response.
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    URL: http://dx.doi.org/10.1007/BF02380576
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    Cantharidin production in a blister beetle (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 171-174 
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    ISSN: 1420-9071
    Keywords: Coleoptera ; Meloidae ; Epicauta funebris ; chemical defense ; biosynthesis ; terpenoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cantharidin, a potent defensive chemical, is present in all ten life stages of the blister beetleEpicauta funebris. The first five larval stages accumulate cantharidin as they feed and grow in size. When disturbed, they exude cantharidin in a milky oral fluid, not in hemolymph which adult beetles reflexively discharge from leg joints. Two subsequent larval stages and the pupa do not feed, grow, regurgitate, or change in their defensive reserves (110 μg cantharidin/insect, regardless of sex). Adult beetles kept in isolation for 60–90 d exhibit a pronounced sexual dimorphism in cantharidin production: the male biosynthesizes about 17 mg of the toxin, representing 10% of his live weight, whereas the female actually loses most of her defensive reserves. But in the wild a female beetle repeatedly acquires cantharidin as copulatory gifts from her mates.
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    Avoidance of competition by spruce bark beetles,Ips typographus andPityogenes chalcographus (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 272-275 
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    ISSN: 1420-9071
    Keywords: Semiochemical ; pheromone ; host selection ; competition ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Bark beetles,Ips typographus andPityogenes chalcographus, attracted by synthetic or natural pheromone to Norway spruce logs,Picea abies, preferred to colonize uninfested logs rather than logs occupied by these beetles, probably as a means of avoiding intra-and interspecific competition. The aggregation pheromone components ofP. chalcographus, chalcogran and methyl (E, Z)-2,4-decadienoate, inhibited the attraction response ofI. typographus to its pheromone components (methyl butenol andcis-verbenol), while the converse was not true. However, verbenone released from colonized bark inhibited pheromonal response ofP. chalcographus.
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    URL: http://dx.doi.org/10.1007/BF01923539
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    Nα-quinaldyl-L-arginine·HCl, a new defensive alkaloid fromSubcoccinella-24-punctata (Coleoptera, Coccinellidae) (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 628-630 
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    ISSN: 1420-9071
    Keywords: Coleoptera ; Coccinellidae ; Subcoccinella-24-punctata ; chemical defense ; quinoline alkaloid ; Nα-quinaldyl-L-arginine·HCl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The isolation of Nα-quinaldyl-L-arginine·HCl (1) from the CoccinellidaeSubcoccinella-24-punctata is reported. The structure, first established on the basis of the analysis of the spectral properties of1, has been confirmed by synthesis. The alkaloid is of endogenous origin and markedly deterrent to ants.
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    URL: http://dx.doi.org/10.1007/BF01969744
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    Is the alkaloid in 2spot ladybirds (Adalia bipunctata) a defence against ant predation? (1993)
    Springer
    Chemoecology 4 (1993), S. 29-32 
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    ISSN: 1423-0445
    Keywords: chemical defence ; alkaloids ; predation ; Coleoptera ; Coccinellidae ; Adalia bipunctata ; Coccinella septempunctata ; Hymenoptera ; Formicidae ; Lasius niger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Garden black ants,Lasius niger L., in a laboratory colony, attacked three species of live ladybirds found near their nest, killing the smaller two species. A second colony was offered artificial diets containing crushed ladybirds of two species, and the ants' choice of feeding site noted. Both the diets were aversive compared to control, but that containing 7spot,Coccinella septempunctata L., was more aversive than the diet containing 2spot,Adalia bipunctata L. The implications of this lesser protection for 2spots in terms of the chemical defence of the species are discussed.
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    URL: http://dx.doi.org/10.1007/BF01245893
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    Performance of first instarChrysophtharta bimaculata larvae (Coleoptera: Chrysomelidae) on nine families ofEucalyptus regnans (Myrtaceae) (1996)
    Springer
    Chemoecology 7 (1996), S. 94-106 
    Details
    ISSN: 1423-0445
    Keywords: herbivory ; plant resistance ; leaf colour ; leaf toughness ; tree growth rate ; leaf nutrients ; terpenes ; Coleoptera ; Chrysomelidae ; Paropsinae ; Chrysophtharta bimaculata ; Myrtaceae ; Eucalyptus regnans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In bagged and unbagged shoot experiments, we investigated the survival and growth rate of first instar larvae ofChrysophtharta bimaculata on 9 families of a natural host,Eucalyptus regnans. Families used had been previously assessed as being either of low or high susceptibility toC. bimaculata damage. In conjunction with larval experiments, we measured 24 tree and leaf characteristics (including foliar elemental concentrations, foliar terpenes, leaf toughness and tree growth rates) and attempted to correlate the plant characters measured with differences in larval performance and previous scorings ofE. regnans family susceptibility. First instar larval growth and survival did not differ significantly across families or between low and high susceptibility family groups (=susceptibility classes), although survival was significantly greater in bagged than unbagged treatments. As predators were in low abundance at the study site, we attribute higher survival rates of larvae in bagged treatments to increased protection from adverse weather conditions. Only one plant character measured, an unidentified foliar phlorglucinol, was significantly negatively correlated with larval survival. Of the 24 plant characters measured, 11 were significantly different between families and 10 were signficantly different between susceptibility classes. Only 4 plant characters were significantly different at both the family and susceptibility class levels;viz. proportion red leaves, tree height at end of season, trunk volume at end of season and relative growth rate based on tree height. Principle Component Analysis using all plant characters measured, or subsets of them, could not separate individual families or susceptibility classes. Our results suggest that herbivore resistance mechanisms inE. regnans do not affectC. bimaculata larvae, but may influence adult feeding and/or oviposition.
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    The use of the tergal gland defensive secretion in male intrasexual aggression of the rove beetle,Aleochara curtula (Coleoptera: Staphylinidae), measured by closed-loop-stripping-analyses and tandem bioassay — mass fragmentography (1996)
    Springer
    Chemoecology 7 (1996), S. 24-33 
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    ISSN: 1423-0445
    Keywords: male aggression ; defensive secretion ; tergal gland ; closed-loop-stripping-analyses ; tandem bioassay ; mass fragmentography ; 2-methyl-1,4-benzoquinone ; Coleoptera ; Staphylinidae ; Aleochara curtula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 2-methyl-1,4-benzoquinone as a target compound of the tergal gland secretion of the rove beetle,Aleochara curtula, was quantified from the reservoirs of individual beetles. Males store less secretion than females, and they evaporate more of the secretion as measured by adsorption from the air (closed-loop-stripping-analysis). The amount of emitted quinone is increased during aggressive interactions of rival males. The pulsed emission of the secretion during aggression is demonstrated by a novel technique combining observation of behavior with the on-line measurement of target fragment ions by mass-spectrometry (tandem bioassay — mass fragmentography). The emission of the secretion is used as a weapon in combats between males and may result in the repulsion of subordinate males from the mating site, but may also serve to mimic females chemically in order to avoid aggressive encounters.
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    URL: http://dx.doi.org/10.1007/BF01240634
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    Quantitative composition of the defensive secretion ofBledius species (Coleoptera: Staphylinidae: Oxytelinae) is adapted to naturally occurring predators (1993)
    Springer
    Chemoecology 4 (1993), S. 63-71 
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    ISSN: 1423-0445
    Keywords: chemical defense ; predator-prey interaction ; synergism ; Coleoptera ; Staphylinidae ; Oxytelinae ; Bledius ; Carabidae ; Dyschirius ; Pogonus ; Dichirotrichus ; Formicinae ; Cataglyphis ; Dermaptera ; Labidura
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The adaptation of defensive secretions to their target organisms was examined for the abdominal gland secretions ofBledius furcatus, B. spectabilis andB. arenarius. Therefore the target organisms of the secretion of theseBledius species (i.e. their predators) had to be identified. At the collection sites examined these were the earwigLabidura riparia, the antCataglyphis bicolor, the flyLispe candicans, different carabids of the generaPogonus, Dichirotrichus, Dyschirius, Bembidion andCalathus and the wading birdsHaematopus ostralegus andCalidris alba. The secretion of the abdominal glands contains the toxin ptoluquinone dissolved in eitherγ-dodecalactone and 1-undecene (B. furcatus andB. spectabilis) or in octanoic acid and octyloctanoate (B. arenarius). The ratio of these solvents is species-specific. Application experiments using some of the natural insect predators (L. riparia, C. bicolor, Pogonus, Di. gustavii, Dyschirius) revealed that these solvent ratios provided a more effective deterrent than other possible ratios. Thus by combining the solvents in certain ratios, the capability of cuticular penetration and therefore the effectiveness of the defensive secretions are adapted to their natural targets.
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    Comparison of the insecticidal effects of water extracted and intact aromatic plants onAcanthoscelides obtectus, a bruchid beetle pest of kidney beans (1994)
    Springer
    Chemoecology 5-6 (1994), S. 1-5 
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    ISSN: 1423-0445
    Keywords: adult mortality ; oviposition inhibition ; larval mortality ; aromatic hydrodistillated and non hydrodistillated plants ; Coleoptera ; Bruchidae ; Acanthoscelides obtectus ; Lamiaceae ; Origanum ; Thymus ; Satureia ; Rosmarinus ; Mentha ; Myrtaceae ; Eucalyptus ; Lauraceae ; Laurus ; Poaceae ; Cymbopogon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Acanthoscelides obtectus (Say) is one of the most damaging pests of kidney beans,Phaseolus vulgaris L. worldwide. However, aromatic plants from the families Lamiaceae, Lauraceae, Myrtaceae and Poaceae can protect kidney beans by direct or delayed insecticidal effect, through increased adult mortality and inhibition of beetle reproduction (both oviposition and adult emergence). The efficiency of hydrodistillated and intact plants fromThymus vulgaris andT. serpyllum, Mentha piperata, Rosmarinus officinalis, Satureia hortensis, Eucalyptus globulus, Laurus nobilis, Origanum vulgare, andCymbopogon nardus was compared. For both extracts,Origanum vulgare had the best effect. The insecticidal effect was induced by more than the essential oils because no significant difference was noticed between distilled and intact plants extract. Inhibition of reproduction was particularly important. These results suggest that lipidic as well as non lipidic, allelochemicals, such as phenolics, or non-protein amino-acids, or flavonoids may be involved in the toxicity of aromatic plants to this beetle.
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    Ladybird defence alkaloids: Structural, chemotaxonomic and biosynthetic aspects (Col.: Coccinellidae) (1994)
    Springer
    Chemoecology 5-6 (1994), S. 173-183 
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    ISSN: 1423-0445
    Keywords: chemical defence ; sequestration ; reflex bleeding ; alkaloids ; chemotaxonomy ; biosynthesis ; repellency ; toxicity ; Coleoptera ; Coccinellidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The defensive mechanisms which protect ladybird beetles (Coccinellidae) against predators are reviewed. Besides behavioural mechanisms, such as thanatosis and reflex bleeding, chemical defence mechanisms are playing a prevalent role. Indeed, ladybirds are protected not only by their smell, but also by repulsive alkaloids, most of which are considered to be of autogenous origin. In a few cases, dietarily-acquired substances are also involved. Particular emphasis is laid on the repellent alkaloids which are contained in the haemolymph of many species. The structures of 34 nitrogen-containing compounds isolated so far are presented, and their distribution within the family is discussed in the light of the most widely accepted classification of these beetles. To conclude, the mode of release of the alkaloids, their variation through the life cycle and their repellent and toxic properties are discussed, as well as the few biosynthetic data yet available.
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    Chemoecological studies of the exocrine glandular larval secretions of two chrysomelid species (Coleoptera):Phaedon cochleariae andChrysomela lapponica (1994)
    Springer
    Chemoecology 5-6 (1994), S. 185-189 
    Details
    ISSN: 1423-0445
    Keywords: oviposition ; repellents ; larval secretion ; Coleoptera ; Chrysomelidae ; Phaedon cochleariae ; Chrysomela lapponica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The exocrine glandular secretions of larvae of the subfamily Chrysomelinae are known to repel conspecific adults, other competitive phytophagous insects and natural enemies. InPhaedon cochleariae, the intraspecific activity of tlc fractions of the larval secretion was tested in order to examine the ecological significance of two fractions containing minor components and a fraction containing the major compound, the cyclopentanoid monoterpene (epi)chrysomelidial. InChrysomela lapponica, the defensive activity of the larval secretion against ants is known from specimens feeding upon willow or birch. The feeding preferences of larvae and adults ofC. lapponica from a Finnish and a Czech population were tested. The Finnish individuals significantly preferred feeding uponSalix borealis, whereas they hardly fed upon birch. The Czech specimens clearly preferred birch (Betula pubescens) to willow species. Application of salicin onto leaves of a willow species free of this phenolglycoside revealed that the Finnish individuals preferred feeding upon leaves with salicin. On the other hand, the Czech individuals avoided feeding upon leaves ofB. pubescens treated with salicin. The chemical composition of the glandular secretion of the Finnish larvae differed from the one of the Czech larvae. GC-MS-analyses of the secretions revealed that salicylaldehyde was the only major component of the secretion of Finnish larvae feeding upon the salicin-containing willowS. borealis. The glandular secretion of the Czech larvae feeding upon birch contained numerous esters of isobutyric acid and 2-methylbutyric acid. When Czech larvae had fed upon a salicin-containing willow (S. fragilis), the major compounds of their secretion were benzoic acid, salicylalcohol and benzoic acid esters; salicylaldehyde was only detected in traces. Thus,C. lapponica individuals from the Finland population adapted so closely to a salicincontaining willow that they clearly prefer this plant for food and that they obviously derive their main larval defensive compound (salicylaldehyde) from their host-plant.
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    Behavior and ecology of Old World Luperini beetles of the genusAulacophora (Coleoptera: Chrysomelidae) (1996)
    Springer
    Chemoecology 7 (1996), S. 150-155 
    Details
    ISSN: 1423-0445
    Keywords: kairomone ; behavior ; cucurbitacin ; Cucurbitaceae ; Coleoptera ; Chrysomelidae ; Luperini ; Aulacophora ; Diabrotica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The attraction of Old World leaf beetles in the genusAulacophora to kairomones and parakairomones which are effective lures for New WorldDiabrotica andAcalymma were investigated. Beetles captured on sticky traps baited with single and multicomponent lures were no different from the control traps for two species ofAulacophora. Yellow colored traps and squash blossoms are attractive toAulacophora beetles which detect sub-microgram quantities of cucurbitacins on silica gel. Leaf feeding behavior and flight activity data are correlated with varietal preference of threeAulacophora species. The common response byDiabrotica andAulacophora to cucurbitacins reinforces the two groups' coevolutionary association with the Cucurbitaceae. The apparent lack of a common response toCucurbita blossom volatiles suggests recent evolutionary pathways are substantially different for these two groups of beetles.
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    Odour profile congruity in two closely related desert tenebrionid beetles: Homology as the basis for a cleptoparasitic relationship? (1996)
    Springer
    Chemoecology 7 (1996), S. 156-161 
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    ISSN: 1423-0445
    Keywords: subsociality ; cleptoparasitism ; mimetism ; odour congruity ; homology ; defensive secretions ; Kalahari desert ; Coleoptera ; Tenebrionidae ; Parastizopus armaticeps ; Eremostibes opacus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Parastizopus armaticeps andEremostibes opacus are two closely related desert tenebrionids which also live in close association, the former having biparental brood care and the latter cleptoparasitising the brood.E. opacus is unable to discriminate between the conspecific and host odour even in the absence of physical contact. Gas chromatographic analysis of headspace volatiles of resting animals showed almost complete qualitative and quantitative odour congruity between them. Comparison of these odour profiles with those of two other tenebrionids sharing the same ecological niche,Gonopus agrestis andHerpiscius sp. (damaralis?) showed that congruity was independent of common foodplant utilisation. It is also independent of common defensive gland secretions. Parallels between resting odour spectra, defensive secretion spectra and systematic status suggest that the origins of congruity lie in odour homology, by means of which the cleptoparasite was able to exploit its host.
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    Volatile substances in the pygidial secretion of gyrinid beetles (Coleoptera: Gyrinidae) (1996)
    Springer
    Chemoecology 7 (1996), S. 191-193 
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    ISSN: 1423-0445
    Keywords: volatile substances ; pygidial secretion ; defence ; communication ; species differences ; Coleoptera ; Gyrinidae ; Gyrinus spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gyrinid beetles are common in freshwater habitats. They have paired pygidial glands with a secretion that contains high molecular weight substances rendering them toxic for predatory fish. In this paper we report on a laboratory study on volatile components released by three different gyrinid species when irritated. The volatile pattern had a clear difference between the different species.Gyrinus substriatus andG. aeratus, both produced 3-methyl-1-butanal and 3-methyl-1-butanol when irritated, but in quite different amounts. The third tested species,G. minutus, did not produce any substances above the detection level. It is suggested that the volatile compounds may be part of the beetles' communication and/or defence system.
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    Sexual dimorphism in the lipid fraction of the defensive secretion ofGastrophysa viridula (Coleoptera: Chrysomelidae) (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 766-770 
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    ISSN: 1420-9071
    Keywords: Gastrophysa viridula ; Chrysomelidae ; Coleoptera ; chemical defence ; acetates ; hydrocarbons ; gas-liquid chromatography ; gas chromatography-mass spectrometry ; sexual dimorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The lipid fraction of the defensive secretion of adultGastrophysa viridula was analyzed by gas-liquid chromatography and gas chromatography-mass spectrometry. Three long chain acetates and two methyl-branched hydrocarbons were identified and their amounts in the secretion of single field-collected and laboratory-reared beetles determined. The composition of the secretion was affected by the sex, reproductive status, and age of the beetle. The ratio of (Z)-11-eicosenyl acetate to (Z)-13-docosenyl acetate ranged from 0.30 to 0.78 in male beetles and from 1.05 to 2.06 in female beetles. The biological significance of this sexual dimorphism is discussed.
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    Female sex pheromone of the longhorn beetleMigdolus fryanus Westwood: N-(2′S)-methylbutanoyl 2-methylbutylamine (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 853-856 
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    ISSN: 1420-9071
    Keywords: Migdolus fryanus ; Coleoptera ; Cerambycidae ; N-2′-methylbutanoyl 2-methylbutylamine ; N-formyll-isoleucine methyl ester ; sex pheromone ; chiral resolution ; dose-response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The first known long-range female-released sex pheromone for the family Cerambycidae is reported fromMigdolus fryanus, a sugarcane pest in South America. Although two female-specific compounds, namely, N-(2′S)-methylbutanoyl 2-methylbutylamine and N-formyll-isoleucine methyl esters were identified, field tests with synthetic chemicals revealed that only the amide was active and that the amino acid derivative neither increased or decreased trap catches by the amide. This is the first identification of amide as a sex pheromone.
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    Apparent transgenerational effects of host plant in the leaf beetle Ophraella notulata (Coleoptera: Chrysomelidae) (1993)
    Springer
    Oecologia 96 (1993), S. 365-372 
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    ISSN: 1432-1939
    Keywords: Coleoptera ; Herbivory ; Host selection ; Maternal effect ; Paternal effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The natural host of Ophraella notulata is Iva frutescens (Asteraceae); its close relative feeds on a related plant, Ambrosia artemisiifolia. We reared beetles on both plants, obtained progeny from the four possible crosses (two sexes X two parental hosts), and reared the progeny on both plant species. Survival to the imaginal stage of progeny reared on Iva varied with both maternal and paternal host. Hatchling feeding response to both plants showed a maternal host X paternal host interaction. Consumption of Ambrosia by adult beetles was, counter to expectation, higher for progeny of Iva-reared males than Ambrosia-reared males. Oviposition response, although based on too few data to be definitive, was peculiar: parental host did not affect oviposition on Ambrosia; on Iva daughters of Iva-reared males laid significantly more eggs than did daughters of Ambrosia-reared males, but only if they had been reared on Iva; those reared on Ambrosia displayed the reverse pattern. We discuss the possibility that nongenetic paternal transmission of host plant effects may explain these results, but offer a somewhat uncomfortable hypothesis of selection as a preferable explanation. An important outcome of the experiment is that it provided no evidence of maternal effects of host plant on offspring feeding or oviposition.
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    Field tests of a balloon-suspended trap system for studying insects in the canopy of tropical rainforests (1994)
    Springer
    Ecological research 9 (1994), S. 357-360 
    Details
    ISSN: 1440-1703
    Keywords: balloon system ; Coleoptera ; pollinators ; tree canopy ; wood borers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A system of suspending traps from a movable balloon was developed and tested on pollinating and wood boring Coleoptera in a tropical rainforest canopy in Pasoh Forest Reserve, Peninsular Malaysia. The balloons, 2.5 m in diameter, were filled with helium gas for levitation and moored in different directions by three pieces of thin rope. Traps in which benzyl acetate and ethanol were used as attractants for the pollinators and wood borers, respectively, were suspended from the balloon at different heights. The trapping results show that the balloon system can be used for studying canopy insects and their vertical distribution on a spatial and temporal scale.
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    URL: http://dx.doi.org/10.1007/BF02348423
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    Evidence for volatile chemical attractants in the beetleMaladera matrida argaman (Coleoptera: Scarabaeidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2673-2685 
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    ISSN: 1573-1561
    Keywords: Maladera matrida ; Coleoptera ; Scarabaeidae ; collection of volatiles ; field trapping ; olfactometer ; attractants ; host plant volatiles ; synergism ; aggregation ; sex pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract TheMaladera matrida beetle (Coleoptera, Scarabaeidae, Melolonthinae), a relatively new species to science, was first identified in Israel in 1983. In the course of field observations it was found that adultM. matrida beetles emerged from the soil at sunset to feed and mate. During the first 20 min of flight, most of the beetles were males. The females emerged shortly afterwards, and aggregations numbering 20–30 individuals with equal proportions of males and females were eventually formed on peanut plants. Laboratory olfactometer bioassays showed that peanut leaves (food) attracted both males and females. Field-trapping experiments and olfactometer studies showed thatM. matrida beetles were highly attracted by live virgin females in the presence of food (cut-up peanut leaves). Another set of field trapping experiments indicated that airborne volatiles produced by live virgin females plus food had the same attracting ability as live virgin females plus food. The attraction exerted by the combination of live virgin females and peanut leave volatiles suggests a synergism effect. Accordingly, we propose a two-stage mechanism of chemical communication in theM. matrida beetles: first, the males cause mechanical damage to the host plant to attract both sexes; later, the females emit attractants (sex pheromone) while eating or shortly thereafter.
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    Mechanism of aggregation behavior inMaladera matrida Argaman (Coleoptera: Scarabaeidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 361-371 
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    ISSN: 1573-1561
    Keywords: Coleoptera ; Scarabaeidae ; Maladera matrida ; aggregation ; plant volatiles ; olfactometer ; attractant ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract AdultMaladera matrida Argaman (Coleoptera: Scarabaeidae: Melolonthinae) males emerge from soil for an active period at dusk, a few minutes before the females. Adults are found during most of the active hours on the foliage in aggregations composed of an equal sex ratio. The mechanism of aggregation behavior ofM. matrida beetles was studied in a Y-shaped olfactometer. No evidence was found for the existence of an aggregation pheromone released either by males or by females, but behavior tests indicate that adultM. matrida beetles, males as well as females, are attracted to volatiles of an injured host plant. The following scenario is suggested: Males emerge daily from soil at dusk, a few minutes before the females, and immediately start feeding. Additional males are attracted to the injured host's volatiles and form aggregations. When females emerge from soil, the attractant volatiles are concentrated in spots, and the females join the aggregations, forming an equal sex ratio.
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    Tasting green leaf volatiles by larvae and adults of Colorado potato beetle,Leptinotarsa decemlineata (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 753-769 
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    ISSN: 1573-1561
    Keywords: Contact chemoreception ; electrophysiology ; taste ; leaf alcohols ; galeal sensilla ; feeding behavior ; host selection ; gustatory coding ; Colorado potato beetle ; Leptinotarsa decemlineata ; Coleoptera ; Chrysomelidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Larvae and adults of the Colorado potato beetle,Leptinotarsa decemlineata (Say), are shown to have galeal gustatory cells that are highly sensitive to distillate of potato leaf extracts, (E)-2-hexen-1-ol, (E)-2-hexenal, and other saturated and unsaturated six-carbon alcohols. In larvae and adults, the sensory response patterns elicited by leaf homogenate, leaf distillate and a mixture of these two extracts differ in subtle ways. Beetle larvae feed most readily on Millipore disks treated with leaf homogenate and the mixture, but they did not feed on disks treated with leaf distillate. The differences in behavioral response and sensory input are used to derive a potential gustatory code that may stimulate different levels of feeding. This code may be disrupted by compounds present in nonhost leaves, thus leading to reduced feeding. Possible interactions of sapid leaf volatiles, amino acids, sugars, and potentially deterrent plant compounds are discussed.
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    Impact of acidic deposition onEncelia farinosa gray (Compositae: Asteraceae) and feeding preferences ofTrirhabda geminata horn (Coleoptera: Chrysomelidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 97-105 
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    ISSN: 1573-1561
    Keywords: Encelia farinosa ; Asteraceae ; Trirhabda geminata ; Coleoptera ; Chrysomelidae ; feeding preferences ; acidic deposition ; plant stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Container grownEncelia farinosa were exposed to three 3-hr episodes of acidic fog (pH 2.5) typical of events in southern California. Adults and larvae of the specialist leaf-feeding herbivore,Trirhabda geminata, preferred to feed on the acidic-treated foliage compared to control fogged (pH 6.3–6.5) foliage. Previous feeding damage on the plants did not affect feeding preference. The acidic-fogged foliage was significantly higher in total nitrogen and soluble protein but not different from control-treated tissue in water content. Stress on native populations of this drought-deciduous shrub caused by atmospheric pollutants may also result in altered feeding ecology of the beetle.
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    Reduced offspring production in bark beetleTomicus piniperda in pine bolts baited with ethanol and α-pinene, which attract antagonistic insects (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 1429-1444 
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    ISSN: 1573-1561
    Keywords: Tomicus piniperda ; Thanasimus formicarius ; Rhizophagus depressus ; Rhizophagus ferrugineus ; Epuraea spp. ; Acanthocinus aedilis ; predators ; competitors ; attractants ; α-pinene ; ethanol ; Scolytidae ; Cleridae ; Rhizophagidae ; Cerambycidae ; Coleoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bolts of Scots pine,Pinus sylvestris L., attacked by the bark beetleTomicus piniperda (L.) were baited with ethanol and α-pinene to attract antagonistic insects and thereby enhance their detrimental effects on the production of bark beetle progeny. Unbaited and caged bolts were included in the experiments as controls. Attraction of beetles to the bolts and subsequent emergence were estimated using traps. Six phloem-feeding species (potential competitors ofT. piniperda) and four predatory species were caught in significantly higher numbers at the baited bolts than at the unbaited ones. The number of offspring and the productivity ofT. piniperda were four to seven times higher in unbaited bolts than in baited bolts. Exclusion of other insects, by using cages, resulted in a nine-fold increase in the number ofT. piniperda offspring per square meter and productivity (offspring per egg gallery) compared with unbaited, exposed bolts.Hylurgops palliatus (Gyll.) (Scolytidae) andRhagium inquisitor (L.) (Cerambycidae) attacked both the baited and unbaited bolts, whereasAcanthocinus aedilis (L.) (Cerambycidae) andPytho depressus (L.) (Pythidae) reproduced almost exclusively in the baited ones. Large numbers of larvae ofThanasimus (Cleridae) andRhizophagus (Rhizophagidae) emerged from both the baited and unbaited bolts. Adults ofPlegaderus vulneratus (Panzer) andCylister linearis (Er.) (Histeridae) emerged almost exclusively from the baited bolts. The low progeny production ofT. piniperda in the baited bolts was attributed largely to the influence of adults ofRhizophagus andEpuraea (Nitidulidae), and larvae ofThanasimus andA. aedilis.
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    Male-produced aggregation pheromone ofCarpophilus obsoletus (Coleoptera: Nitidulidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 1483-1493 
    Details
    ISSN: 1573-1561
    Keywords: Carpophilus obsoletus ; sap beetle ; Coleoptera ; Nitidulidae ; aggregation pheromone ; hydrocarbon ; tetraene ; date
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Males ofCarpophilus obsoletus Erichson produce an aggregation pheromone to which both sexes respond. The pheromone was identified by GC-MS as (2E,4E,6E,8E)-3,5,7-trimethyl-2,4,6,8-undecatetraene (1), which is also a minor constituent of the pheromone blends ofC. hemipterus (L.),C. freemani Dobson, andC. lugubris Murray. The pheromone was synergized in wind-tunnel bioassays by propyl acetate, a “host-type” coattractant. In a dose-response study, 50 pg of1, plus propyl acetate, was significantly more attractive than just propyl acetate. Pheromone emission from groups of 65 males, feeding on artificial diet, averaged 2.2 ng/male/day. Emissions from individual males were larger, averaging 72 ng/day and ranging as high as 388 ng/day. Synthetic1 was tested in a date garden in southern California (500 µg/rubber septum), using fermenting whole-wheat bread dough as the coattractant. The pheromone plus dough attracted significantly more beetles than dough alone (means were 4.2 and 0.0 beetles per week per trap). Captured beetles were 54% females. Field trap catches were highest during the months of July and August.
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    Identification and synthesis of female sex pheromone of Oriental beetle,Anomala orientalis (Coleoptera: Scarabaeidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2415-2427 
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    ISSN: 1573-1561
    Keywords: Anomala orientalis ; Oriental beetle ; Coleoptera ; Scarabaeidae ; sex pheromone ; (Z)- and (E)-7-tetradecen-2-one ; DMDS derivatization ; flight tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Females of the Oriental beetle,Anomala orientalis (Waterhouse), release a sex pheromone composed of a 9:1 blend of (Z)- and (E)-7-tetradecen-2-one. The double-bond position of the pheromone was determined by DMDS derivatization and interpretation of the fragmentation patterns produced by monounsaturated ketones. In a sustained-flight tunnel, males responded by flying toward female beetles and attempting to copulate with them. Both effluvium and whole-body extracts of OB females were analyzed, and the activity was found only in the airborne extracts. Flight-tunnel bioassays also showed that a synthetic 90:10Z/E blend on a rubber septum was attractive and that the responses of males to this blend were equivalent toZ isomer alone, but much better than to the singleE isomer.
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    Physiological sources of variation in chemical defense ofOreina gloriosa (Coleoptera: Chrysomelidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 395-410 
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    ISSN: 1573-1561
    Keywords: Oreina gloriosa ; Coleoptera ; Chrysomelidae ; chemical defense ; cardenolides ; quantitative variation ; aging ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defensive secretion of the alpine chrysomelidOreina gloriosa is a complex mixture of mainly cardenolides and tyrosine betaine. Individually sampled secretions of adult laboratory-reared and field-collected beetles were analyzed by reverse-phase HPLC; 16 secretion components were quantified. Quantities and concentrations of different components were significantly affected by the age, sex, and reproductive status of individual beetles. Aging was correlated with marked increases (up to 4.4-fold) and decreases (up to 2.7-fold) of quantities and concentrations of several components. Differences between the sexes were smaller, but quantities of all components and concentrations of several components were larger in laboratory-reared females than in males. There was less of one component of the secretion in mated than unmated females, but the concentrations of four secretion components were higher (up to 1.6-fold) in mated females.
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    Toxicity of allyl isothiocyanate-amended soil toLimonius californicus (Mann.) (Coleoptera: Elateridae) wireworms (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1033-1046 
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    ISSN: 1573-1561
    Keywords: Rapeseed ; Brassica spp. ; allyl isothiocyanate ; glucosinolates ; allelochemicals ; Limonius californicus (Mann.) ; Coleoptera ; Elateridae ; toxicity ; sublethal effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Acute toxicity of soil amended with allyl isothiocyanate (AITC) to three size classes ofLimonius californicus (Mann.) wireworms was determined in the laboratory. Wireworms were exposed to AITC at initial concentrations of 120–300 nmol/g soil for one day. During this time, extractable AITC concentrations decreased by 66 to 93 %. Probit analysis estimated LC50 values of 238 and 226 nmol/g soil at one day posttreatment for medium and large wireworms, respectively. For small wireworms, LC50 values decreased from 211 to 157 nmol/g soil during 1–137 days posttreatment. Sublethal concentrations of AITC significantly reduced feeding activity of treated wireworms at three posttreatment times and over the entire 137 days. Wireworm weight was not significantly affected by AITC. The potential exists to use glucosinolate-containing plant tissue as an isothiocyanate (ITC) source to reduce crop damage caused byL. californicus wireworms.
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    Host recognition by entomopathogenic nematodes: Behavioral response to contact with host feces (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1219-1231 
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    ISSN: 1573-1561
    Keywords: Host recognition behavior ; entomopathogenic nematodes ; feces ; Spodoptera exigua ; Lepidoptera ; Noctuidae ; Popillia japonica ; Coleoptera ; Scarabaeidae ; Blattella germanica ; Blatteria ; BlattellidaeAcheata domesticus ; Orthoptera ; Gryllidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Host recognition by entomopathogenic nematodes may occur through contact with insects' excretory products, cuticle, or gut contents. We analyzed the behavioral responses of four species of entomopathogenic nematodes during contact with feces of natural or experimental hosts. Host recognition by nematodes was manifested in alterations in the frequency and/or duration of one or more search parameters including forward crawling, headwaving, body-waving, stopping, backward crawling, head-rubbing, and headthrusting.Heterorhabditis bacteriophora andSteinernema glaseri showed behavioral responses to contact with feces of their natural hosts,Spodoptera exigua (Lepidoptera) andPopillia japonica (Coleoptera), and to the experimental hosts,Acheata domesticus (Orthoptera) andBlatella germanica (Blatteria).Steinernema carpocapsae responded only toB. germanica feces, whereas5. scapterisci did not significantly respond to any of the insect species. During contact with cockroach feces, all nematodes, exceptS. scapterisci, showed avoidance behavior. We suggest that ammonia present in cockroach feces is inhibitory to nematodes. Specific host recognition by entomopathogenic nematodes may be an important mechanism to maintain host affinities.
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    Unusual periodicity of sex pheromone production in the large black chaferHolotrichia parallela (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1381-1391 
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    ISSN: 1573-1561
    Keywords: Holotrichia parallela ; large black chafer ; scarab beetle ; Coleoptera ; Scarabaeidae ; isoleucine methyl ester ; linalool ; sex pheromone ; circabidian periodicity ; pheromone titer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (R)-(−)-Linalool was identified as a minor component sex pheromone of the scarab beetleHolotrichia parallela (Coleoptera: Scarabaeidae). Field evaluations revealed that, although not attractive per se, (R)-(−)-linalool enhances the attractiveness of the major sex pheromone,L-isoleucine methyl ester (LIME). Analyses of the pheromone titers in the glands of field-collected females demonstrated the occurrence of peak levels of 48-hr (“circabidian”) periodicity. The levels of LIME in the glands of 45-day-old virgin females increased over three times from the scototo the photophase of a calling day, but the amounts of (R)-(−)-linalool did not significantly change. Virgin females had in average two times more LIME and 3.6 times more (R)-(−)-linalool than the average amount found in the field-captured beetles throughout the season.
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    Field evaluation ofAnomala schonfeldti ohaus (Coleoptera: Scarabaeidae) synthetic sex pheromone (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1453-1459 
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    ISSN: 1573-1561
    Keywords: Anomala schonfeldti ; Popillia japonica ; scarab beetle ; Coleoptera ; Scarabaeidae ; 2-(E)-nonenol ; sex pheromone ; mark-and-recapture ; field test ; mass trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Synthetic 2-(E)-nonenol, previously identified as the sex pheromone ofAnomala schonfeldti (Coleoptera: Scarabaeidae), is demonstrated to be very attractive to males in the field. Nevertheless, no significant differences were found between treatments with 1, 5, 10, and 20 mg dosages. Males ofA. schonfeldti were more significantly attracted to traps at 30 cm high than at 90 cm. Although the observed behavior seemed to indicate a trend of more attraction to buried traps than those placed at 30 cm, there was no statistical difference between the two treatments. Pheromone-baited traps caught significantly more beetles than traps containing three virgin females. Over 70% of released beetles were recaptured in six traps surrounding the point of release and separated from each other by 50 m, suggesting a possible use of the pheromone (in combination with floral compounds) in mass trapping.
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    Grain weevil,Sitophilus granarius (L.): Antennal and behavioral responses to male-produced volatiles (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 1639-1654 
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    ISSN: 1573-1561
    Keywords: Sitophilus granarius ; grain weevil ; Coleoptera ; aggregation pheromone ; electroantennogram ; coupled GC-EAG ; behavioral bioassay ; circular dichroism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Coupled GC-EAG techniques have been applied to the study of volatiles from the grain weevil,Sitophilus granarius. for the first time. The size of EAG response was independent of the sex of the responding insect but was consistently larger to extracts of males than those of females. This difference was reflected in a behavioral preference for the male extracts by mated adults of both sexes tested together and virgin adults of both sexes tested separately. The GC-EAG results provide evidence for two materials that are released specifically by the males. Using circular dichroism. one has been found to be identical stereochemically with the (2S,3R)-sitophilate reported by others as the aggregation pheromone in a different strain. This enhances the prospects for the development of a single pheromone lure that would be generally applicable whatever the origin of the strain. The small amount of sitophilate found in the males suggests that it is not stored in large amounts. The other material, present in such a small amount that it has yet to be fully characterized, elicits a higher antennal activity than sitophilate and may have a significant role to play in enhancing the trap catch of this economically important pest.
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    Dabbing and shooting of benzo- and naphthoquinone secretions: Defensive strategies of bark-inhabiting aleocharine (col.: Staphylinidae) and tenebrionid (col.: Tenebrionidae) beetle larvae (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1337-1354 
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    ISSN: 1573-1561
    Keywords: Coleoptera ; Staphylinidae ; Tenebrionidae ; larvae ; defensive glands ; quinone ; naphthoquinone ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The abdominal glands of three bark-inhabiting larvae of generaBolitochara, Leptusa (Staphylinidae), andHypophloeus (Tenebrionidae) were studied chemically and morphologically. Behavior of the larvae indicated that secretion is emitted only after severe disturbance of the larvae. These mechanical contacts may also occur incidentally with coinhabiting nonpredatory arthropods when the beetle larvae move within small interstices under bark. Depending on the species, the secretions contained 1,4-benzoquinone derivatives and three 6-alkyl-naphthoquinones dissolved within various alkanes, alkenes, ethyl-, isopropyl-, and isoamylesters. More erratically distributed gland constituents also detected were acetophenone, benzyl propionate, and methyl hydroxybenzoate. In the laboratory, synthetic quinone-containing solutions simulating those found inLeptusa andBolitochara larvae acted as strong topical irritants and caused further damage to last-stageCalliphora vomitoria larvae if hydrocarbons or esters were used as solvents. The natural secretions ofHypophloeus versipellis elicited considerable mortality in two subcortical sciarid larvae cooccurring with tenebrionid larvae. Bioassay and secretion chemistry of the Staphylinidae/Tenebrionidae larval secretions indicated that they are typical defensive secretions that act topically. Morphological data characterized Bolitocharini larvae as possessing protuberant abdominal tergites supplied with an interiorly situated gland reservoir. After mechanical contact, the defensive secretion is topically applied to other arthropods by dabbing this tergal protuberance on targets. The defensive gland ofHypophloeus versipellis is unusual in possessing a movable reservoir opening situated at the anterior border of tergite IX. By this peculiar gland morphologyHypophloeus larvae are capable of shooting secretion droplets frontally from their slightly depressed dorsal abdominal surface without bending their abdominal tips dorsally. This seems an adaptation to the interstitial habitat of the larvae. The types of defensive glands and their phylogenetic value in Aleocharinae/Tenebrionidae larvae are discussed.
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    Response on the ground of bark beetle and weevil species colonizing conifer stumps and roots to terpenes and ethanol (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1393-1403 
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    ISSN: 1573-1561
    Keywords: Hylastes cunicularius ; Hylastes brunneus ; Hylastes opacus ; Dryocoetes autographus ; Hylobius abietis ; Hylobius pinastri ; α-pinene ; terpenes ; turpentine ; ethanol ; ground traps ; Coleoptera ; Scolytidae ; Curculionidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Responses of threeHylastes species,Dryocoetes autographus, and twoHylobius species to terpenes and ethanol were studied in field experiments on clear-cut forest sites in Sweden using baited ground traps.α-Pinene alone did not attract any of the six species. A terpene blend (spruce turpentine consisting mainly ofα-pinene,β-pinene, and 3-carene) attractedHylastes cunicularius, H. brunneus, andHylobius abietis in some experiments, but not in others. The attractiveness of ethanol also varied; the only species consistently attracted wasH. abietis. Baits containing both terpenes and ethanol, particularly the combination of spruce turpentine and ethanol, were attractive to all species exceptHylobius pinastri. InH. abietis, the terpene plus ethanol/ ethanol catch ratios increased during early summer. Seasonal differences in catch levels were observed inH. cunicularius andH. abietis. The addition ofα-pinene reduced the attractiveness of the combination of spruce turpentine and ethanol toH. cunicularius, H. opacus, andD. autographus. The differences in response to the volatiles between species are probably related to differences in reproductive behavior and host preferences.
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    What factors influence the diversity of saproxylic beetles? A multiscaled study from a spruce forest in southern Norway (1996)
    Springer
    Biodiversity and conservation 5 (1996), S. 75-100 
    Details
    ISSN: 1572-9710
    Keywords: saproxylic ; Coleoptera ; spruce forest ; spatial scale ; conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: The diversity in different groups of obligate saproxylic beetles was related to ecological variables at three levels of spatial scale in mature spruce-dominated forest. The variables were connected to: (i) decaying wood, (ii) wood-inhabiting fungi, (iii) the level of disturbance, (iv) landscape ecology, and (v) vegetational structure. Several strong relationships were found at medium (1 km2) and large scales (4 km2), while only weak relationships were found at a small scale (0.16 ha; 1 ha=104 m2). This may be explained by the local variations in habitat parameters and the high mobilities of many beetle species. Factors connected to decaying wood and wood-inhabiting fungi were clearly the most important factors at all scale levels. In particular, the variables diversity of dead tree parts, number of dead trees of large diameter and number of polypore fungi species increased the species richness of many groups and increased the abundance of many species. Eight species were absent below a certain density of decaying wood per 1 or 4 km2. Former extensive cutting was a negative factor at large scale, probably because of decreasing recolonization with increasing distance to the source habitats. Thinning reduced the diversity of species associated with birch. The development of guidelines favouring the diversity of saproxylic beetles are discussed below.
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    URL: http://dx.doi.org/10.1007/BF00056293
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    Reproductive biology ofFrangula alnus (Rhamnaceae) in southern Spain (1994)
    Springer
    Plant systematics and evolution 193 (1994), S. 173-186 
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    ISSN: 1615-6110
    Keywords: Rhamnaceae ; Frangula ; Diptera ; Coleoptera ; Hymenoptera ; Mecoptera ; Breeding system ; phenology ; pollination biology ; protandry ; reproductive success ; self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various aspects of the reproductive biology of the perennial hermaphroditeFrangula alnus Mill. were studied in two populations located in the province of Cádiz, southern Spain. Flowering extends from the second week of May to early July. The small, whitegreenish, entomophilous flowers are incompletely protandrous and last 8–10 days, but pollen transfer takes place only on days 1–3. A very weak, pleasant odour along with nectar and pollen attract over 45 insect species, of which 21 are probable pollinators. These are mainlyDiptera but also includeHymenoptera and the unusual (as flower visitors)Mecoptera. Reproduction is exclusively sexual and strictly xenogamous, with pollen transfer depending solely on insect vectors. Although flower morphology and individual flower phenology do not fully prevent self-pollination, and geitonogamy can easily take place, the level of autogamy was negligible. Therefore, some self-incompatibility mechanism is operative in this species. Only 2.8% of open-pollinated flowers set fruit. At the flower level, fruit initiation was apparently limited by availability of cross-pollen, which in turn seemed influenced by the structure of the population. The seed/ovule ratio in ripe fruits was ca. 50%. Predispersal (maternal) reproductive success (percent ovules becoming filled seeds) was 1.42%. For mature individuals this corresponded to ca. 430 to 1560 potential offspring per year.
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    Analysis of divergence of Alphitobius diaperinus satellite DNA — roles of recombination, replication slippage and gene conversion (1994)
    Springer
    Molecular genetics and genomics 242 (1994), S. 297-304 
    Details
    ISSN: 1617-4623
    Keywords: Repetitive sequences ; Sequence variability ; Satellite DNA evolution ; Coleoptera ; X70332
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Satellite DNA is highly abundant in Alphitobius diaperinus (Tenebrionidae, Coleoptera), comprising 25% of the total genomic DNA. Sequence analysis reveals an average GC content of 50.8% and the presence of three different groups of satellite monomer variants, tH1, tH2 and tH3 with corresponding lengths of 123, 128 and 126 bp. Their mutual homologies range between 65 and 81 %. Sequence comparison shows that the monomer variant tH2 has been formed by a recombination process between tH1 and tH3, which have a low average homology of only 65.15%. The longest stretch of 100% homology between the recombining units is 17 bp and is located 3′ to the predicted recombination site. There is also an indication from sequence analysis that replication slippage and gene conversion play a part in the formation of satellite units and contribute to their divergence. The tH1, tH2 and tH3 monomer variants are organized in higher order repeating structures: a dimer, composed of tH1, and tH3, and a trimer containing tH1, tH2 and tH3 in series. The dimeric and trimeric repeat units furthermore create three higher order satellite subfamilies. Two of them contain either tandemly arranged dimers or trimers, while the third one is composed of both types of repeats, mutually interspersed.
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    Multiple-chromosome sex systems in the darkling beetles Blaps gigas and Blaps gibba (Coleoptera, Tenebrionidae) (1996)
    Springer
    Genetica 97 (1996), S. 225-233 
    Details
    ISSN: 1573-6857
    Keywords: karyology ; banded chromosomes ; Coleoptera ; darkling beetles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied mitotic and meiotic chromosomes in the males of two species of Blaps: B. gigas and B. gibba. Karyological characteristics such as the occurrence of a multivalent configuration at diakinesis and two types of metaphase-II spreads support the notion that multiple-chromosome sex systems involving five chromosomes in B. gigas and eight chromosomes in B. gibba have developed in these species. Results obtained by means of silver staining and C-banding techniques suggest that the complex sex systems occurring in B. gigas and B. gibba may have originated from exchanges of terminal ribosomal genes among the Y chromosome and some autosomes.
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    URL: http://dx.doi.org/10.1007/BF00054629
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    Aggregation pheromones inDryocoetes affaber (Mann.) (Coleoptera: Scolytidae): stereoisomerism and species specificity (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 111-124 
    Details
    ISSN: 1573-1561
    Keywords: Semiochemicals pheromones ; Dryocoetes affaber ; Dryocoetes confusus ; Coleoptera ; Scolytidae ; enantiomers ; diastereoisomers ; exo-brevicomin ; endo-brevicomin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chemical analysis of whole body extracts and volatiles produced by feeding malesDryocoetes affaber (Mann.) disclosed (+)-exo-brevicomin and (+)-endo-brevicomin [(+)EXOB and (+)ENDOB], as the major insect-produced potential pheromones. Laboratory bioassays and field-trapping experiments demonstrated that (+)ENDOB is the main pheromone component, and (-)ENDOB has an inhibiting effect. EXOB either as (+) or (±) appears to be a multifunctional pheromone. It has a synergistic effect in blends of EXOB and ENDOB in ratios up to 1:1, and it is inhibitory at higher ratios. (-)EXOB was inactive. The most attractive blend forD. affaber was a 1:2 blend of (+)EXOB and (+)ENDOB. When this blend was compared with a 9:1 blend, the best known blend forDryocoetes confusus Swaine, the responses by beetles of each of the two species were highly specific, providing evidence for pheromonal exclusion between the two congenerics. We conclude that the combined effect of chirality and the ratio of geometrical isomers of brevicomin determines both the level of response and the species-specificity of the chemical signal inD. affaber.
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    URL: http://dx.doi.org/10.1007/BF02065994
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    Comparison of walking locomotory reactions of two forms ofCallosobruchus maculatus males subjected to female sex pheromone stimulation (Coleoptera: Bruchidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2917-2930 
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    ISSN: 1573-1561
    Keywords: Callosobruchus maculatus ; Coleoptera ; Bruchidae ; flightless form ; flight form ; tubular olfactometer ; walking behavior ; intermittent stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A comparison of the walking locomotory reactions of flightless and flight formCallosobruchus maculatus males when subjected to an air current scented with female sex pheromone was undertaken in a tubular olfactometer. The pheromone was delivered to the males either as short pulses or as a continuous flow. To analyze the males' reactions, three behavioral sequences were defined (sequence 1: male sensitivity/arousal; sequence 2: male reactivity; sequence 3; male progression and source location). Although flightless and flight form males were sensitive and reactive in all experiments, their locomotory displacement differed depending on the stimulus conditions. The flightless form males' response remained roughly the same whatever the stimulus conditions (i.e., they always reached the pheromone source). In contrast, the flight form males displayed a markedly reduced response when subjected to a continuous stimulation, indicating that intermittent on-off pheromone stimulation is required in order to sustain their upwind walking progress. This effect could be the result of sensory adaptation and/or habituation in the central nervous system of the flight form, requiring a flickering signal that is unnecessary for the flightless one.
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    Aggregation pheromone of palmetto weevil,Rhynchophorus cruentatus (F.) (Coleoptera: Curculionidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 505-515 
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    ISSN: 1573-1561
    Keywords: Coleoptera ; Curculionidae ; Rhynchophorus cruentatus ; palmetto weevil ; S. palmetto ; aggregation pheromone ; 5-methyl-4-octanol ; cruentol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract 5-Methyl-4-octanol is the major aggregation pheromone of the palmetto weevil,Rhynchophorus cruentatus (F.). The pheromone (cruentol) was identified by coupled gas chromatographic-electroantennographic (GC-EAD) analysis of male-produced volatiles, coupled GC-mass spectrometry (MS) in electron impact and chemical ionization mode, and coupled GC-high resolution MS. In laboratory and field assays, a diastereomeric mixture of synthetic cruentol greatly enhanced attraction of weevils to cabbage palmetto,Sabal palmetto (Walter), stem tissue, indicating that cruentol and host volatiles are synergistically attractive. An attractive lure in combination with efficient traps should facilitate development of semiochemical-based management forR. cruentatus.
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    Oxidative responses in soybean foliage to herbivory by bean leaf beetle and three-cornered alfalfa hopper (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 639-650 
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    ISSN: 1573-1561
    Keywords: Soybean ; lipoxygenase ; peroxidase ; polyphenol oxidase ; trypsin inhibitor ; ascorbate oxidase ; oxidative stress ; Lepidoptera ; Noctuidae ; Helicoverpa zea ; corn earworm ; Cerotoma trifurcata ; bean leaf beetle ; Coleoptera ; Chrysomelidae ; Spissistilus festinus ; three-cornered alfalfa hopper ; Homoptera ; Membracidae ; induced resistance ; interspecific competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Variation in induced responses in soybean is shown to be dependent, in part, upon herbivore species. Herbivory by the phloem-feeding three-cornered alfalfa hopper caused increases in the activities of several oxidative enzymes including lipoxygenases, peroxidases, ascorbate oxidase, and polyphenol oxidase. Bean leaf beetle defoliation caused increased lipoxygenase activity, but had little effect upon peroxidase, polyphenol oxidase, ascorbate oxidase, or trypsin inhibitor levels in either field or greenhouse studies. In one field experiment, prior herbivory by the bean leaf beetle subsequently reduced the suitability of foliage to the corn earwormHelicoverpa zea. The contribution of these findings to emerging theories of insect-plant interactions is discussed.
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    Influence of white pine watering regimes on feeding preferences of spring and fall adults of the white pine weevilPissodes strobi (Peck) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 831-847 
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    ISSN: 1573-1561
    Keywords: Pissodes strobi ; white pine weevil ; Coleoptera ; Curculionidae ; Pinus strobus ; white pine ; plant vigor ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Spring and fall adults of the white pine weevil,Pissodes strobi (Peck), were exposed in no-choice and two-choice tests to bark from water-stressed and non-water-stressed white pine (Pinus strobus L.), which had also been exposed or not exposed to weevil attack. This experiment demonstrated that the weevils could discriminate between bark from water-stressed white pine and preferred bark from the nonstressed plants. The weevils also preferred bark from nonstressed plants that were previously exposed to weevil damage. Spring and fall adults displayed the same feeding preferences. No sex differences were found in feeding preferences. Less nitrogen, phosphorus, and potassium were found in bark from the nonstressed plants, and the potassium level was higher in damaged plants. We expect that the biological performance of the weevil should be favored by vigorously growing plants rather than by stressed plants.
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    Geometrical and optical isomerism of pheromones in two sympatricDryocoetes species (Coleoptera: Scolytidae), mediates species specificity and response level (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2169-2182 
    Details
    ISSN: 1573-1561
    Keywords: Semiochemicals ; pheromones ; Dryocoetes confusus ; Dryocoetes affaber ; Coleoptera ; Scolytidae ; enantiomers ; diastereoisomers ; exo-brevicomin ; endo-brevicomin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a field-trapping experiment, western balsam bark beetles,Dryocoetes confusus Swaine, were highly attracted to a 5∶1 mixture of (±)-exo-and (±)-endo-brevicomin. Beetles in the sympatric speciesD. affaber (Mann.), were best attracted to a 1∶1 blend of these semiochemicals [either (±)∶(±) or (±)∶(±)], suggesting that both geometrical isomers are pheromone components in these species. In laboratory bioassays and further field experiments, attraction ofD. confusus was greatest when the (+) enantiomers of both geometrical isomers of brevicomin were presented in a 9∶1 ratio. Responses by maleD. confusus to attractive mixtures were reduced in the presence of (−)-exo-brevicomin. Exploitation of the complete range of variability in pheromone structure (both geometrical and optical isomerism) would allow for optimization and regulation of response levels within a species and also could maintain reproductive isolation among sympatric congeneric species primarily through production and response to species-specific blends.
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    Stereospecific antennal response by red turpentine beetle,Dendroctonus valens to chiral monoterpenes from ponderosa pine resin (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2193-2202 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Dendroctonus valens ; EAG ; electroantennogram ; enantiomer ; kairomone ; host attraction ; bark beetle ; α-pinene ; β-pinene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The antennal response ofDendroctonus valens to host monoterpenes from the resin of ponderosa pine was studied using the electroantennogram (EAG) technique. Male and female beetles were given a single dose of each of 11 different monoterpenes. Response amplitude to the different compounds did not vary between sexes and was generally well correlated with results from field attraction studies. Response to (S)-(−)-β-pinene was greatest. The relative amplitude of the responses to the (R)-(+) and (S)-(−) enantiomers of a-pinene, however, were reversed from their relative attractiveness in the field. A dose-response study was conducted for the (R)-(+) and (S) -(−) enantiomers of a-pinene, plus a reciprocal differential saturation test with successive doses of first one enantiomer ofα-pinene and then the other. Comparison of EAG traces suggests different receptors for the two stereoisomers ofα-pinene. Differential saturation curves suggest that while one set of receptors may respond to both enantiomers, some receptors respond only to the (S)-(−) enantiomer.
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    Aggregation pheromone ofCarpophilus antiquus (Coleoptera: Nitidulidae) and kairomonal use ofC. lugubris pheromone byC. antiquus (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2203-2216 
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    ISSN: 1573-1561
    Keywords: Carpophilus antiquus ; C. lugubris ; C. freemani ; Coleoptera ; Nitidulidae ; pheromone ; kairomone ; hydrocarbon ; tetraene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Males ofCarpophilus antiquus Melsheimer (Coleoptera: Nitidulidae) emit an aggregation pheromone that was found to be a novel hydrocarbon, (3E,5E,7E,9E)-6,8-diethyl-4-methyl-3,5,7,9-dodecatetraene. A synthetic scheme and spectra (mass and proton NMR) are given for the compound. Beetles produced the pheromone when feeding on a variety of media, including the brewer's yeast-based artificial diet, fermenting whole-wheat bread dough, corn, and prunes; live baker's yeast was generally added to the food media. Males held individually produced, on average, 25 × more pheromone per beetle than males held in groups of 10 or more. Pheromone was not produced until males were at least 5 days old but was still detected from the oldest beetles tested (47 days). In field tests, the pheromone was attractive to both sexes ofC. antiquus, and it was synergized by food volatiles: A combination of pheromone and fermenting whole wheat dough attracted 2.5× more beetles than pheromone alone, but dough by itself was not significantly more attractive than the control. Semiochemical interactions were studied amongC. antiquus and two other sympatric species for which pheromones are known,C. lugubris Murray andC. freemani Dobson.C. antiquus responded readily to the pheromone ofC. lugubris, but all other interspecific responses to the pheromones were weak. In a sample of naturally infested corn ears, the presence ofC. antiquus was strongly associated with the presence ofC. lugubris, as would be expected if the pheromone ofC. lugubris serves as a kairomone forC. antiquus.
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    Identification of branched alkanes in lipids ofLeptinotarsa decemlineata say andTribolium destructor by GC-MS: A comparison of main-beam and link-scanned spectra (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2197-2212 
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    ISSN: 1573-1561
    Keywords: Insects hydrocarbons ; alkanes ; Colorado beetle ; Leptinotarsa decemlineata Say ; Tribolium destructor ; Coleoptera ; Teneboionidae ; choysomelidae ; mass spectrometry ; linked scanning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Branched hydrocarbons were identified in the lipids ofLeptinotarsa decemlineata Say andTribolium destructor by gas chromatography, ordinary electron impact mass spectrometry, and linked, scanned, daughterion monitoring. This methodology allowed us to revise our earlier results based only on GC-MS data confirming the existence of only monomethyl-, dimethyl-, and trimethylalkanes in the hydrocarbons ofL. decemlineata Say. The hydrocarbons fromTribolium destructor consist ofn-alkanes, 3-methylalkanes, internally branched monomethylalkanes and dimethylalkanes. Daughter-ion monitoring can be particularly important for distinguishing between incidentally overlapped GC peaks of hydrocarbons from different series. A trace, for example, of dimethylalkane coeluating withn-alkane was easily identified in GC peak of hydrocarbon mixture ofT. destructor. Link scans confirmed also molecular weights for the compounds without molecular ions in the mass spectra. Structural assignment of the compounds were verified by comparison of the experimental and calculated values of the GC retention Kovats indexes (KI).
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    Behavioral and electrophysiological responses of the banana weevilCosmopolites sordidus to host plant volatiles (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 267-277 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Curculionidae ; Cosmopolites sordidus ; banana ; weevil ; Musa sp. ; pseudostem ; rhizome ; EAG ; olfactometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male and femaleCosmopolites sordidus were attracted to freshly cut banana rhizome and pseudostem in a still-air olfactometer. Females responded similarly to odors from a comparatively resistant and from a susceptible cultivar of banana, when presented as either freshly cut tissue or as Porapak-trapped volatiles. Females were also attracted to rotting banana pseudostem and to volatiles collected from it. Males and females gave similar responses to host tissue in both the behavioral bioassay and to collected volatiles in EAG recordings. Weevils did not respond, either behaviorally or electrophysiologically, to a synthetic mixture of mono- and sesqiterpenes, which made up over 9% of the volatiles collected from pseudostem.
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    Olfactory receptor cell responses ofIps grandicollis (eichhoff) (Coleoptera: Scolytidae) to intra- and interspecific behavioral chemicals (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 699-712 
    Details
    ISSN: 1573-1561
    Keywords: Ips ; Dendroctonus ; Coleoptera ; Scolytidae ; interspecific attraction ; behavioral chemicals ; single olfactory cells ; electrophysiology ; pheromones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electrophysiological recordings from antennal olfactory receptor cells were obtained fromIps grandicollis. Recordings were made from olfactory receptor cells from nine regions of the antennae in response to stimulation with the semiochemicalsα-pinene, frontalin,endo-brevicomin, verbenone,trans-verbenol,cis-verbenol, ipsdienol, and ipsenol. In many cases, up to two cells were recorded concurrently from the same location. When compared to males, females had a greater percentage of cells responsive to the primary pheromones ofDendroctonus frontalis, frontalin andtrans-verbenol, and ofIps spp., ipsdienol and ipsenol. Among females, more cells responded totrans-verbenol and theIps-produced volatiles than to host or otherD. frontalis-produced compounds. Olfactory cells of males responded mostly tocis-verbenol, followed byα-pinene, verbenone,trans-verbenol, andendo-brevicomin. Of those cells responsive primarily to one compound, the greatest percentage were responsive totrans-verbenol in females and to verbenone in males. The response of the antennal olfactory receptor cells to semiochemicals used by male and femaleI. grandicollis is consistent with the presence of these compounds during the host colonization period for each sex. Our results, which show a lack of specificity in most pheromone and host odor receptor cells, is in contrast with previously published accounts of olfactory receptor cell specificity in otherIps species.
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    Evidence for allelochemical attraction of the coffee berry borer,Hypothenemus hampei, by coffee berries (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 763-769 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Hypothenemus hampei ; host selection ; kairomones ; olfaction ; Coffea sp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Petri dish choice tests conducted on the coffee berry borer (CBB),Hypothenemus hampei, showed that females were able to discriminate between coffee berries at different ripening stages. A Y-shaped glass olfactometer was used to demonstrate that coffee berries emitted volatile chemicals that elicited upwind movement by female CBB. Olfactometer tests with three different solvent extracts of berries showed that at least some of the attractive chemical(s) released by the coffee berries could be extracted with acetone.
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    The effect of short-range host odor stimuli on host fruit finding and feeding behavior of plum curculio adults (Coleoptera: Curculionidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 825-835 
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    ISSN: 1573-1561
    Keywords: Conotrachelus nenuphar ; Coleoptera ; Curculionidae ; host odor ; host location ; feeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In laboratory assays, we investigated responses of female plum curculios (PCs),Conotrachelus nenuphar (Herbst), to host and nonhost fruit or leaf odor when PCs were crawling on experimental tree branchlets or twigs. In choice tests where test specimens were hung from the ends of a wooden crosspiece, PCs made significantly more visits to host plum fruit than to plum leaves, nonhost tomato fruit, wax models of plum fruit, or blanks (wire). In similar tests, PCs made significantly more visits to plum leaves compared to nonhost maple leaves or to blanks. PCs in test chambers that contained host or nonhost odor were significantly more prone to feed on wax plum models in the presence of odor from host fruit or host leaves compared to odor from nonhost fruit or leaves or a water blank. In choice tests offering alternating cluster types on an apple branchlet, PCs visited leaf clusters bearing a host apple fruit more than leaf clusters without a fruit. In tests to assay the distance at which PCs can detect an individual host fruit, PCs crawled from the central stem of an apple branchlet onto a side stem significantly more often when an apple fruit on a side stem was hung 2 cm from the central stem compared to 4 or 8 cm away. Our combined results suggest that PCs use host fruit odor to locate host fruit at close range.
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    Interactions between components of the aggregation pheromone during chemotaxis by the bark beetleIps paraconfusus (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 863-879 
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    ISSN: 1573-1561
    Keywords: Olfaction ; ipsenol ; ipsdienol ; cis-verbenol ; chemotaxis ; perception ; discrimination ; neural models ; logit models ; stimulus-response models ; Ips paraconfusus ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The male-produced aggregation pheromone inIps paraconfusus is composed of three compounds. Female bark beetles were exposed to combinations of these compounds, presented as point sources in an enclosed, circular arena. By itself,cis-verbenol (cV) had no effect on the number of beetles that reached the source. Either ipsenol (Ip) alone or ipsdienol (Id) alone strongly increased the number that reached the source, with Id producing a dose-response curve with a much steeper slope. cV moved the onset of the response to Id to higher doses of Id, but the response rose more rapidly after onset than when cV was absent. Overall, cV inhibited the effect of Id except at the highest dose. cV affected the onset of the response to Ip little or none, but strongly increased the slope of the response, synergizing the effect of Ip. The responses to combinations of Id and Ip were related to the log of a linear combination of their doses. The results are consistent with a model where Id and Ip act at a single site of action, but with different potencies, while cV appears to modify the effects of Id and Ip, rather than affecting the site of action directly.
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    Green leaf volatiles as antiaggregants for the mountain pine beetle,Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae) (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 1861-1875 
    Details
    ISSN: 1573-1561
    Keywords: Mountain pine beetle ; Dendroctonus ponderosae ; Coleoptera ; Scolytidae ; antiaggregant ; verbenone ; green leaf volatiles ; 1-hexanol ; (E)-2-hexen-1-ol ; (E)-3-hexen-1-ol ; (Z)-2-hexen-1-ol ; (Z)-3-hexen-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We tested the hypothesis that green leaf volatiles act as antiaggregants for the mountain pine beetle (MPB),Dendroctonus ponderosac Hopkins. In coupled gas chromatographic-electroantennographic detection (GC-EAD) analysis MPB antennae responded to 30 ng doses of all six-carbon green leaf alcohols tested [1-hexanol, (E)-2-hexen-1-ol, (Z)-2-hexen-1-ol, (E)-3-hexen-1-ol, and (Z)-3-hexen-1-ol], but not to the aldehydes, hexanal or (E)-2-hexenal, or to alcohol or aldehyde homologues with more or fewer than six carbon atoms. In field trapping experiments a blend of green leaf alcohols [1-hexanol, (Z)-2-hexen-1-ol, (E)-3-hexen-1-ol and (Z)-3-hexen-1-ol] effectively disrupted the response to attractive semiochemicals; a blend of the aldehydes hexanal and (E)-2-hexenal was inactive. The two best disruptants. (E)-2-hexen-1-ol and (Z)-3-hexen-1-ol, reduced catches of both sexes to levels not significantly different from catches in unbaited control traps. They also reduced the attack on trees baited with attractive MBP pheromones to a level not significantly different from that on unbaited control trees. Neither of the clerid predators captured,Enoclerus sphegeus (F.) norThanasimus undatulus (Say), was repelled by green leaf volatiles. Our results suggest that green leaf alcohols are promising disruptants which may be used to supplement the antiaggregation pheromone, verbenone, in protecting single high-value trees as well as carefully selected stands with low-level populations of MPBs.
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    URL: http://dx.doi.org/10.1007/BF02028509
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    Scarab beetleAnomala albopilosa albopilosa utilizes a more complex sex pheromone system than a similar speciesA. cuprea (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 2001-2010 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scarabaeidae ; GC-EAD ; methyl benzoate ; 2-(E)-nonenal ; 2-(E)-nonenol ; (R, Z)-5-(−)-(oct-1-enyl)oxacyclopentan-2-one
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Four compounds have been identified as sex pheromone constituents of the scarab beetleAnomala albopilosa albopilosa, namely (R, Z)-5-(−)-(oct-1-enyl)oxacyclopentan-2-one (buibuilactone), 2-(E)-nonenol, 2-(E)-nonenal, and methyl benzoate (in the ratio 10:3:3:1). The diel rhythm of pheromone release inA. a. albopilosa showed a peak at the beginning of the scotophase, which is also a peak of mating activity. On the other hand, the similar speciesA. cuprea utilizes a two-constituent sex pheromone, having a common major component, but the pheromone is released both during scoto-and photophase. Temporal difference in mating activity and pheromone release along with chemical diversity seem to form the basis for maintaining species specificity in pheromonal communication betweenA. a. albopilosa andA. cuprea.
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    Effect of ethanol and α-pinene on response of ambrosia beetle,Trypodendron lineatum, to lineatin-baited funnel and drainpipe traps (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 2187-2196 
    Details
    ISSN: 1573-1561
    Keywords: Trypodendron lineatum ; Scolytidae ; ambrosia beetle ; lineatin ; ethanol ; α-pinene ; pheromone ; semiochemical ; Coleoptera ; trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The response ofT. lineatum to ethanol and α-pinene, when used with the pheromone lineatin, was tested for two trap types. Funnel traps, which are passive barrier traps, caught significantly more beetles than drainpipe traps, which require an active response by the beetle to enter the trap. However, the response ofT. lineatum to the semiochemical treatments did not significantly differ for the two trap types. Treatments that included α-pinene with the pheromone, either with or without ethanol, caught significantly moreT. lineatin than those with the pheromone alone. When ethanol and or α-pinene were added to the pheromone significantly more female beetles were trapped than with pheromone alone. Male-female ratios were significantly lower for both types of traps when ethanol was included in the bait than for lineatin alone or with α-pinene. A higher percentage of male beetles entered the drainpipe traps than was captured with funnel traps.
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    URL: http://dx.doi.org/10.1007/BF02029539
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    Male-produced aggregation pheromone ofCarpophilus mutilatus (Coleoptera: Nitidulidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 107-118 
    Details
    ISSN: 1573-1561
    Keywords: Carpophilus mutilatus ; sap beetle ; Coleoptera ; Nitidulidae ; aggregation pheromone ; hydrocarbon ; triene ; date ; host volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Males ofCarpophilus mutilatus Erichson produce an aggregation pheromone to which both sexes respond. The pheromone includes two hydrocarbon components, (3E,5E,7E)-5-ethyl-7-methyl-3,5,7-undecatriene (1) and (3E,5E,7E)-6-ethyl-4-methyl-3,5,7-decatriene (2). These were emitted in a 10∶1 ratio and in a total amount of ca. 5 ng per feeding male per day. All tested doses of1 and2, from 0.03 to 30 ng, were more attractive than controls in wind-tunnel tests, but there was no evidence of synergism between these trienes. Dramatic synergism between the pheromone and a food-type coattractant occurred in the field, however. In a date garden in southern California, traps with a combination of synthetic1 and fermenting whole-wheat bread dough attracted 22 times more beetles than dough by itself and 295 times more than1 by itself. Volatile collections from males also contained three oxygenated compounds that were absent from females. One of these was tetradecanal (ca. 5 ng per male per day), but the structures of the other two are presently undetermined (0.8 and 1.1 ng per male per day). No function for these was demonstrated. One compound originating in the artificial diet, 2-phenylethanol, was particularly attractive in the wind-tunnel bioassay, as was the chromatographic solvent, methanol.
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    URL: http://dx.doi.org/10.1007/BF00987476
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    Photoisomerization of antiaggregation pheromone verbenone: Biological and practical implications with respect to the mountain pine beetle,Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1749-1759 
    Details
    ISSN: 1573-1561
    Keywords: Mountain pine beetle ; Dendroctonus ponderosae ; Coleoptera ; Scolytidae ; antiaggregation pheromone ; verbenone ; chrysanthenone ; photoisomerism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Release of the antiaggregation pheromone, verbenone, at 3.8 mg/ day from a concentrated source within a multiple-funnel trap completely inhibited response by the mountain pine beetle (MPB),Dendroctonus ponderosae Hopkins, to attractive semiochemical lures. When aerial applications were simulated and verbenone was released at the same rate from beads lying in a 2×2-m area on the forest floor 15–35 cm below a trap, the response of the MPB was inhibited by only 50%. This reduced inhibition may be explained in part by the photoisomerism of verbenone. When exposed to full sunlight on two occasions, the times required for 50% of verbenone vapors to be converted to chrysanthenone were 75 and 100 min, respectively. Trap and tree-baiting experiments indicated no biological activity of chrysanthenone. Rapid photoisomerization could reduce the concentration of verbenone below biologically active levels and would allow the MPB to colonize trees close to already occupied hosts, contributing to the characteristic clumped distribution of MPB attack. The rate of verbenone photoisomerization may vary according to geographic location, stand elevation and density, and should be considered before verbenone is applied to control the MPB and other bark beetles.
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    Emetic voidance of stomach lining induced by massive beetle ingestion in a beluga whale (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1833-1836 
    Details
    ISSN: 1573-1561
    Keywords: Beluga whale ; Delphinapterus leucas ; Coleoptera ; Carabidae ; emesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00982311
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    Male response to natural sex pheromone ofMigdolus fryanus westwood (Coleoptera: Cerambycidae) females as affected by daily climatic factors (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2347-2351 
    Details
    ISSN: 1573-1561
    Keywords: Migdolus fryanus ; Coleoptera ; Cerambycidae ; mating behavior ; sex pheromone ; climatic factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Males of the sugarcane borer,Migdolus fryanus (Coleoptera: Cerambycidae), are attracted to females by means of a sex pheromone. Mating usually occurs during a few days from October to March under field conditions in São Paulo State, Brazil. This work reports on mating of this species as affected by daily climatic factors, during a single nuptial flight. Maximum male capture by the natural sex pheromone occurred from 10∶00 to 11∶00 AM at air and soil temperatures of 30.0°C and relative humidity of 57.0%. As these temperatures increased, females burrowed into the soil, as they are more sensitive to heat than males. Thus, it was concluded that sex pheromone-mediated mating in this cerambycid is directly affected by temperatures of air and soil.
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    Laboratory and field evidence for maleproduced aggregation pheromone inRhynchophorus cruentatus (F.) (Coleoptera: Curculionidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1195-1203 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Curculionidae ; Rhynchophorus cruentatus ; Sabalpalmetto ; aggregation pheromone ; olfactometer ; field trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Laboratory and field assays were conducted to determine if palmetto weevil,Rhynchophorus cruentatus (F.), adults produce an aggregation pheromone. Attraction of females in a Y-tube olfactometer to conspecific males was greater than to clean air. Male and female attraction to conspecific male volatiles combined with host-palm,Sabal palmetto (Walter), volatiles was greater than to host-palm volatiles alone. Similarly, more weevils were caught in the field in traps baited with conspecific males plus host-palm tissue than in similar traps baited with only males, or palm tissue, or females, or females plus palm tissue. These results suggest thatR. cruentatus males produce an aggregation pheromone(s) that is highly attractive to conspecific adults of both sexes when combined with host-palm volatiles. This study is an important step towards understanding the chemical ecology ofR. cruentatus.
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    Insecticidal effect of essential oils from mediterranean plants uponAcanthoscelides Obtectus Say (Coleoptera, Bruchidae), a pest of kidney bean (Phaseolus vulgaris L.) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1233-1244 
    Details
    ISSN: 1573-1561
    Keywords: Essential oils ; Labiatae ; Umbelliferae ; Lauraceae ; citrus limon ; Cymbopogon nardus ; alyptus globulus ; istica fragrans ; insecticidal ; effect ; LC50 ; Acanthoscelides obtectus Say ; Bruchidae ; Coleoptera ; terpenoids ; benzenoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The bioactivity of 22 essential oils from aromatic and medicinal plants was tested uponAcanthoscelides obtectus Say (Coleoptera, Bruchidae), a pest of kidney bean (Phaseolus vulgaris L.). The insecticidal effect was evaluated by determination of 24- and 48-hr LC50 and LC50 (from 1.50 mg/ dm3 to more than 1000 mg/dm3). Isoprenoids and phenylpropanoids were identified by gas chromatography. The most efficient essential oils were extracted from plants belonging to Labiatae.Origanum marjorana andThymus serpyllum essential oils were the most toxic.
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    The scarab beetleAnomala cuprea utilizes the sex pheromone ofPopillia japonica as a minor component (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1303-1313 
    Details
    ISSN: 1573-1561
    Keywords: Japanese beetle ; cupreous chafer ; GC-EAD ; (R,Z)-5-(−)-(oct-1-enyl)oxacyclopentan-2-one ; (R,Z)-5-(−)-(dec-1-enyl)oxacyclopentan-2-one ; sex pheromone ; Anomala cuprea ; Popillia japonica ; Coleoptera ; Scarabaeidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract GC-EAD analyses revealed that the scarab beetleAnomala cuprea, the cupreous chafer, utilizes, in addition to the previously identified major sex pheromone (R,Z)-5-(−)-(oct-1-enyl)oxacyclopentan-2-one, a minor component, (R,Z)-5-(−)-(dec-1-enyl)oxacyclopentan-2-one, which has been previously identified as the sex pheromone of the Japanese beetle. Release of the sex pheromone blend did not significantly differ when collected from feeding or starving female beetles, nor did it differ from volatiles collected in the scoto- and photophase. However, after mating, the amount and the ratio of the two components changed. Field tests revealed that traps baited with the synthetic sex pheromone captured more beetles than traps containing only virgin females. Based on field experiments, 10 mg of a 90∶10 blend of the pheromone was suggested as appropriate for monitoring of the cupreous chafer, although the optimal ratio for attractiveness is yet to be established. The occurrence of minor components in the pheromone system of other scarab beetles is also discussed.
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    Reproductive biology of the yellow-spotted longicorn beetle,Psacothea hilaris (pascoe) (Coleoptera: Cerambycidae). III. Identification of contact sex pheromone on female body surface (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 259-270 
    Details
    ISSN: 1573-1561
    Keywords: Cuticular hydrocarbon ; contact pheromone ; sex pheromone ; mating behavior ; Coleoptera ; Cerambycidae ; Psacothea hilaris ; (Z)-21-methyl-8-pentatriacontene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A major component of female contact sex pheromone of the yellow-spotted longicorn beetle,Psacothea hilaris (Pascoe), was isolated from the elytra and identified as (Z)-21-methyl-8-pentatriacontene. The synthetic compound released the typical mating behavior including holding, mounting, and abdominal bending in males, although its activity was considerably lower than the extract of female elytra when treated on a gelatin capsule as an artificial female model.
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    URL: http://dx.doi.org/10.1007/BF02055097
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    Studies on Bacillus thuringiensis strains isolated from Swedish soils: insect toxicity and production of B. cereus-diarrhoeal-type enterotoxin (1994)
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 406-409 
    Details
    ISSN: 1573-0972
    Keywords: Bacillus cereus-diarrhoeal-type enterotoxin ; Bacillus thuringiensis ; Coleoptera ; Diptera ; Lepidoptera ; thuringiensin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract At moderate concentration, 23 of 40 strains of Bacillus thuringiensis isolated from Sweden were toxic to Trichoplusia ni and five were toxic to Aedes aegypti. Five of the strains were toxic to Diabrotica undecimpunctata at high concentration, two were toxic to Heliothis virescens at low concentration and five produced thuringiensin (formerly called β-exotoxin). No strain was toxic towards the beet armyworm Spodoptera exigua at low concentration. Twenty-three of the strains produced a B. cereus-diarrhoeal-type enterotoxin.
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    Release of ethylene from pruned olive logs: Influence on attack by bark beetles (Coleoptera, Scolytidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2513-2521 
    Details
    ISSN: 1573-1561
    Keywords: Semiochemicals ; kairomones ; ethylene ; ethrel ; Olea europaea ; olive tree ; Phloeotribus scarabaeoides ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In recently pruned olive logs, an increase in ethylene release has been observed between 48 and 72 hr after pruning. The values reached, as well as the duration of ethylene release, varied greatly from one log to another. PioneerPhloeotribus scarabaeoides females have shown a preference for logs in which ethylene emission was higher. In logs treated with ethrel, a significant increase in ethylene emission was observed, together with a greater period of release. Therefore, the use of logs treated with ethrel could be of great importance in the control of this pest of olive trees.
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    2-(E)-nonen-1-ol: Male attractant for chafersAnomala vitis Fabr. andA. dubia Scop. (Coleoptera: Scarabaeidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2481-2487 
    Details
    ISSN: 1573-1561
    Keywords: Anomala vitis ; Anomala dubia ; chafer ; Coleoptera ; Scarabaeidae ; Melolonthidae ; Rutelinae ; attractant ; 2-(E)-nonen-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Traps baited with 2-(E)-nonen-1-ol alone or in combination with other compounds caught large numbers of males of both the vine chafer,Anomala vitis Fabr. and the margined vine chafer,A. dubia Scop. (Coleoptera: Scarabaeidae), vineyard and orchard pests. In a dosage test, the largest numbers were caught by traps baited with 10 mg of 2-(E)-nonen-1-ol, which was the highest dosage tested. This is the first report on male attractants for chafer species occurring in Europe.
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    Repeatability of female response to ipsdienol enantiomeric mixtures by pine engraver,Ips pini (Coleoptera: Scolytidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2611-2622 
    Details
    ISSN: 1573-1561
    Keywords: Ips pini ; Coleoptera ; Scolytidae ; repeatability ; female choice ; response phenotype ; bark beetle ; pheromone ; communication systems ; multiple measurements ; individual variation ; ipsdienol ; enantiomers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Repeatability, a measure of the proportion of variance in a character that occurs among rather than within individuals, is assessed for the phenotypic trait of female response preference for enantiomeric blends of ipsdienol inIps pini at two pheromone concentrations—1 µg and 5 μg of ipsdienol. Average female response shows greater repeatability at the higher pheromone dosage when females are tested in two successive sets of five assays than when assayed in three successive sets or at the lower dosage. Repeatability within each set of five assays is highest for the first set and decreases thereafter. Thus the response phenotype of females for enantiomeric blends of ipsdienol in this experiment is context dependent; female choice of an enantiomeric blend differed between dosages and among sets of assays.
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    Response ofDendroctonus brevicomis Le Conte (Coleoptera: Scolytidae) to different release rates and ratios of aggregation semiochemicals and the inhibitors verbenone and ipsdienol (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 2931-2941 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Dendroctonus brevicomis ; western pine beetle ; aggregation ; verbenone ; ipsdienol ; inhibitors ; antiaggregants ; doseresponse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The response of western pine beetle,Dendroctonus brevicomis Le Conte, to different release rates of the aggregation semiochemicals (attractants)exo-brevicomin, frontalin, and myrcene and the inhibitors verbenone and ipsdienol was investigated. Release rates of verbenone ranging from 0.18 mg/ 24 hr to 1.2 mg/24 hr did not result in significant reductions in mean trap catch ofD. brevicomis. In contrast, very low release rates of ipsdienol (0.02–0.4 mg/24 hr) significantly reduced trap catch compared to controls. The combination of verbenone and ipsdienol, released at rates above 0.09 and 0.02 mg/24 hr, respectively, resulted in significantly lower trap catches ofD. brevicomis in attractant-baited traps. Results of an experiment testing a factorial combination of different release rates of verbenone and attractants suggest that response is not ratio-specific. The response curve ofD. brevicomis to the levels of verbenone was similar across all levels of attractants, while the response to equivalent ratios of attractants to verbenone was not similar, suggesting that the behavior of the beetles is primarily influenced by the absolute release rate of verbenone.
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    Aggregation pheromone of Australian SAP beetle,Carpophilus davidsoni (Coleoptera: Nitidulidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 3207-3219 
    Details
    ISSN: 1573-1561
    Keywords: Aggregation pheromone ; triene ; tetraene ; hydrocarbon ; Coleoptera ; Nitidulidae ; Carpophilus davidsoni ; Australian sap beetle ; trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A male-produced aggregation pheromone was identified for the Australian sap beetle,Carpophilus davidsoni Dobson (Coleoptera: Nitidulidae), by bioassay-guided fractionation of volatiles collected from feeding beetles. The most abundant components were: (2E,4E,6E)-5-ethyl-3-methyl-2,4,6-nonatriene, (3E,5E,7E)-6-ethyl-4-methyl-3,5,7-decatriene, (2E,4E,6E,8E)-3,5,7-trimethyl-2,4,6,8-undecatetraene, and (2E,4E,6E,8E)-7-ethyl-3,5-dimethyl-2,4,6,8-undecatetraene. The relative abundance of these components in collections from individual males feeding on artificial diet was 100:7:9:31, respectively. Pheromone production began within several days after males were placed onto diet medium and continued for at least 20 weeks. Peak production was 〉3 µg total pheromone per male per day. Males in groups of 50–60 emitted less pheromone (the peak level was 0.09 µg per beetle per day), and the emissions from groups contained relatively little tetraene (proportions of the components listed above were 100:7:2:7, respectively). Three additional trienes and one additional tetraene were identified in minor amounts; the entire eight-component male-specific blend is qualitatively identical and quantitatively similar to that of the North American sibling species,C. freemani Dobson. A synthetic blend of the four major components on rubber septa, prepared to emit in the same proportions as from individual males, was highly attractive in the field when synergized with fermenting whole-wheat bread dough. Cross-attraction was observed in the field involving the pheromones ofC. davidsoni, C. hemipterus (L.), andC. mutilatus Erichson. Potential uses of the pheromones in pest management are discussed.
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    Long-chain free fatty acids: Semiochemicals for host location by western corn rootworm larvae (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 3335-3344 
    Details
    ISSN: 1573-1561
    Keywords: Diabrotica virgifera virgifera ; fatty acids ; linoleic acid ; oleic acid ; stearic acid ; semiochemical ; attractants ; western corn rootworm ; host location ; Coleoptera ; Chrysomelidae ; Zea mays ; kairomone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A bioassay-driven sequential fractionation scheme was used to isolate fractions of a crude dichloromethane maize seedling extract behaviorally active to larvae of the western corn rootworm,Diabrotica virgifera virgifera LeConte. (Z,Z)-9,12-Octadecadienoic (linoleic) acid, (Z)-9-octadecenoic (oleic) acid, and octadecanoic (stearic) acid were identified from a purified fraction of maize extract that was attractive to western corn rootworm larvae in choice tests with equal levels of carbon dioxide on both sides of the choice. When synthetic linoleic, oleic, and stearic acids were tested together in the amounts and proportions found in the attractive fraction (1000, 800, and 300 ng of linoleic, oleic, and stearic acids, respectively), significantly more western corn rootworm larvae were found on the side with synthetic free fatty acids plus carbon dioxide than on the side with carbon dioxide alone. Results of the choice-test bioassays were not significantly different when the synthetic blend of free fatty acids was substituted for the purified maize fraction. Neither the purified extract nor the synthetic blend was behaviorally active in preliminary single-choice experiments without carbon dioxide. Linoleic, oleic, and stearic acids were also tested individually in the choice test bioassay with carbon dioxide on both sides of the choice to determine a dose-response curve. Linoleic and oleic acid each had one dose that was significantly attractive in conjunction with carbon dioxide on both sides of the choice, but stearic acid was not active in the doses tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02033730
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  • 92
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    Production of cardenolides versus sequestration of pyrrolizidine alkaloids in larvae ofOreina species (Coleoptera, Chrysomelidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 555-568 
    Details
    ISSN: 1573-1561
    Keywords: Oreina spp. ; Coleoptera ; Chrysomelidae ; larval defense ; cardenolides ; pyrrolizidine alkaloids ; sequestration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Adult leaf beetles of the genusOreina are known to be defended either by autogenously produced cardenolides or by pyrrolizidine alkaloids (PAs) sequestered from the food plant, or both. In this paper we analyze larvae of differentOreina species and show that the larvae contain the same defensive toxins as the adults in quantities similar to those released in the adults' secretion. Both classes of toxins are found in the body and hemolymph of the larvae, despite their different origins and later distribution in the adults. Larvae of sequestering species differed in their PA patterns, even though they fed on the same food plants. The concentration in first-instar larvae of a PA-sequestering species was similar to that in fourth-instar larvae. In all stages examined, the amount of PAs per larva did not greatly exceed the estimated uptake of one day. Eggs of two oviparous species contained large concentrations of the adult's toxins, while neonates of a sequestering larviparous species had no PAs.
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    URL: http://dx.doi.org/10.1007/BF02059597
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  • 93
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    Ethyl propionate: Synergistic kairomone for african palm weevil,Rhynchophorus phoenicis L. (Coleoptera: Curculionidae) (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 889-897 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Rhynchophorus phoenicis ; Elaeis quineensis ; kairomone ; synergism ; oil palm ; palm weevil ; palm volatiles ; host selection ; primary attraction ; aggregation pheromone ; 3-methyl-octan-4-ol ; ethyl acetate ; ethyl propionate ; isobutyl propionate ; ethyl butyrate ; ethyl isobutyrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Small trunk pieces of a freshly felled 10-year-old oil palm,Elaeis quineensis (Jacq.), were placed in a modified Nalgene desiccator, and volatiles captured for six days on Porapak Q. Gas chromatographic (GC) analysis of Porapak-Q-trapped volatiles with both flame ionization (FID) and electroantennographic detection (EAD) using male or femaleR. phoenicis antennae revealed several EAD-active compounds. They were identified as: ethyl acetate, ethyl propionate, isobutyl propionate, ethyl butyrate, and ethyl isobutyrate. In field experiments in the La Me Research Station, Côte d'Ivoire, ethyl propionate (50 mg/24 hr) but not all esters combined (50 mg/24 hr each) significantly increased capture ofR. phoenicis in pheromone-baited (3 mg/24 hr) traps. One kilogram of 1- to 3-day-old palm tissue was significantly more effective than ethyl propionate in enhancing pheromone attraction. Superior attraction of palm tissue may be attributed to additional as yet unknown semiochemicals. Alternatively, release rates and/or ratios of synthetic volatiles differed from those of palm tissue at peak attraction.
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    URL: http://dx.doi.org/10.1007/BF02059585
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  • 94
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    Behavioral and electrophysiological response of cabbage seed weevils (Ceutorhynchus assimilis) to conspecific odor (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 979-989 
    Details
    ISSN: 1573-1561
    Keywords: Ceutorhynchus assimilis ; Coleoptera ; Curculionidae ; olfactometer ; sex pheromone ; aggregation pheromone ; electroantennogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The responses ofCeutorhynchus assimilis Payk. (Coleoptera, Curculionidae) to the odor of overwintered and new generation weevils were studied using an olfactometer, choice tests in a laboratory cage, field tests using sticky traps baited with live weevils, and by electroantennograms (EAG's). Unmated male weevils and, to a lesser extent, female weevils of the overwintered generation were attracted to the odor of live unmated over-wintered female weevils. New generation weevils exhibited no behavioral response to conspecific odor. Male and female weevils of the overwintered generation exhibited positive EAGs to hexane extracts of overwintered female weevils, whereas EAGs of new-generation weevils of either sex were unresponsive to these extracts. This suggests that the unmated female weevils from the overwintered generation produce a volatile chemical or chemicals that attracts unmated male and female weevils. The new generation of female weevils does not produce this attractive chemical before overwintering, and male and female weevils of this generation can not detect the chemical(s) via their antennal chemoreceptors until they have undergone their overwintering period.
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    URL: http://dx.doi.org/10.1007/BF02059736
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  • 95
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    Repellent, toxic, and food protectant effects of pithraj,Aphanamixis polystachya extracts against pulse beetle,Callosobruchus chinensis in storage (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 899-908 
    Details
    ISSN: 1573-1561
    Keywords: Aphanamixis polystachya ; pithraj ; repellent ; contact toxicity ; food protectant ; Callosobruchus chinensis ; Coleoptera ; Bruchidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ground leaves, bark, seeds, and four seed extracts of pithraj,Aphanamixis polystachya (family Meliaceae), a locally grown plant in Bangladesh, were evaluated for their repellency, contact toxicity, and food protectant efficacy against adult pulse beetle (Callosobruchus chinensis L.). The seed extracts showed poor repellent effects, but high contact toxicity to adults at 72 hr after application. The ground leaves, bark, and seeds provided good protection for mung beans against pulse beetles, and the seed powder greatly reduced the F1 progeny and seed damage rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02059586
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  • 96
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    Leaf surface extracts ofSolanum berthaultii hawkes deter colorado potato beetle feeding (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 991-1007 
    Details
    ISSN: 1573-1561
    Keywords: Leptinotarsa decemlineata ; Colorado potato beetle ; Coleoptera ; Chrysomelidae ; Solanum berthaultii ; glandular trichomes ; feeding deterrents ; host preference ; plant resistance to insects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Leaf rinses ofS. berthaultii PI 473334 with methylene chloride were deterrent to feeding by the Colorado potato beetle when applied toS. tuberosum tuber and leaf disks. When the leaf rinse was separated into its nonvolatile and volatile fractions and applied to tuber disks, the nonvolatile fraction was highly deterrent, while the volatile fraction reduced consumption, but not significantly compared to the controls. A hexane leaf rinse was not deterrent to feeding, while an acetone rinse was approximately twofold more deterrent than the methylene chloride rinse when applied to leaf disks. Three cycles of bioassay-guided, reversed-phase open-column fractionation of an acetone leaf rinse yielded a relatively polar fraction with low deterrent activity, and two nonpolar fractions exhibiting higher specific activity. Reversed-phase preparative HPLC of these fractions yielded seven active fractions among the 10 assayed. Subsequent analytical HPLC indicated that two fractions each contained a single UV-absorbing compound, while another represented a mixture of at least four compounds. The remaining fractions were composed of complex mixtures of possibly ionic or polymeric compounds that were poorly resolved by HPLC.
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    URL: http://dx.doi.org/10.1007/BF02059737
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  • 97
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    Composition of larval secretion ofChrysomela lapponica (Coleoptera, Chrysomelidae) and its dependence on host plant (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 1075-1093 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Chrysomelidae ; Chrysomela lapponica ; larval secretion ; defense
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defensive secretion ofChrysomela lapponica larvae, which is produced by nine pairs of exocrine dorsal glands, has been chemically analyzed. TheC. lapponica larvae were kept in the laboratory on leaves of either birch (Betula pendula), alder (Alnus glutinosa), or willow (Salix fragilis). Larvae developed normally on birch and willow, whereas those on alder died within a few days. GC-MS analyses of the secretion of larvae on birch and willow revealed that the composition of this secretion differs distinctly from the known ones of several otherChrysomela species feeding exclusively on Salicaceae. In the exocrine secretion of larvae on birch, 69 compounds were identified, which included the main components isobutyric acid, 2-methylbutyric acid, and esters of the two. Several of the esters have not been reported previously from nature. The alcoholic components of the esters may be hydrolysis products ofBetula glycosides. Most components of the secretion of larvae feeding on birch were also found in the secretion of larvae feeding on willow. In addition, major amounts of benzoic acid and salicylalcohol were present in the secretion of the larvae feeding on willow.C. lapponica obviously acquires salicylalcohol by hydrolysis of salicin from willow leaves. However, in contrast to otherChrysomela species,C. lapponica larvae oxidize only traces of salicylalcohol to salicylaldehyde. The repellent activity of single authentic compounds of the secretion of larvae feeding on birch and willow, respectively, was tested in laboratory bioassays with ants (Myrmica sabuleti). Biosynthetic pathways to some identified compounds are suggested and discussed under evolutionary and functional aspects.
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    URL: http://dx.doi.org/10.1007/BF02059744
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  • 98
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    A new bioassay for testing plant extracts and pure compounds using red flour beetleTribolium castaneum Herbst (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 1161-1177 
    Details
    ISSN: 1573-1561
    Keywords: Bioassay ; red flour beetle ; Tribolium castaneum ; Coleoptera ; Tenebrionidae ; ECI ; Minthostachis mollis ; Melaleuca quinquenervia ; Sapindus saponaria ; α-pinene ; β-pinene ; eugenol ; kaurenic acid ; sparteine ; phagodepression ; phagostimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We designed a new bioassay to test plant extract activity against stored product pests. Plant compounds were added to feed disks composed of wheat flour and yeast and fed to the red flour bettle (Tribolium castaneum). By measuring insect mass, disk mass, and insect mortality over time it was possible to calculate a phagodepression index, an antifeedant index, the amount of treatment chemical ingested by the beetles, the mortality rate, and the efficiency of conversion of ingested food. The assay was performed for 60 hr to allow for possible habituation effects and to discriminate between phagodeterrency and physiological stress caused by treatments. α- and β-Pinene, eugenol, kaurenic acid, sparteine, essential oils ofMinthostachis mollis andMelaleuca quinquenervia, and extracts ofSapindus saponaria were tested. Using this assay we detected the presence of both phagodepressant and phagostimulant compounds inS. saponaria extracts, and we quantified the pronounced effects of sparteine onT. castaneum.
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    URL: http://dx.doi.org/10.1007/BF02059751
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    The scarab beetleAnomala albopilosa sakishimana utilizes the same sex pheromone blend as a closely related and geographically isolated species,Anomala cuprea (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 1667-1676 
    Details
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scarabaeidae ; (R,Z)-5-(−)-oct-1-enyl)oxacyclopentan-2-one ; (R,Z)-5-(−)-(dec-1-enyl)oxacyclopentan-2-one ; (R,E)-5-(−)-(oct-1-enyl)oxacyclopentan-2-one ; Anomala albopilosa sakishimana ; Anomala octiescostata ; Anomala cuprea ; pheromone release ; GC-EAD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two components were identified in the sex pheromone system of the green chafter,Anomala albopilosa sakishimana Nomura: (R,Z)-5-(−)-(oct-1-enyl)oxacyclopentan-2-one (buibuilactone) and (R,Z)-5-(−)-(dec-1-enyl)oxyacyclopentan-2-one (japonilure), which have been previously identified as sex pheromone constituents ofA. cuprea andA. octiescostata. A female-specific minor component, (R,E)-5-(−)-(oct-1-enyl)oxacyclopentan-2-one, did not seem to be involved in pheromonal communication because it was not EAD active, but its role remained unclear. A synthetic blend of the two components captured significantly more beetles than any other treatments. Nevertheless, the fact that both the synthetic sex pheromone and field-captured female beetles were weak lures convinced us that the sex pheromone system may be only part of a complex communication system, probably involving plant volatiles. Although the sex pheromone was released during both the scotophase and photophase, there was an increase of 60% in the photophase.
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    URL: http://dx.doi.org/10.1007/BF02059888
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  • 100
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    Kairomone from dandelion,Taraxacum officinale, attractant for scarab beetleAnomala octiescostata (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 1697-1704 
    Details
    ISSN: 1573-1561
    Keywords: cis-3-Hexenyl acetate ; benzaldehyde ; phenylacetaldehyde ; benzyl alcohol ; phenethyl alcohol ; phenylacetonitrile ; benzyl benzoate ; anethol ; geraniol ; phenethyl propionate ; kairomone ; sex pheromone ; dandelion ; Taraxacum officionale ; Coleoptera ; Scarabidae ; Anomala oritescostata ; scarab beetle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The attraction of the scarab beetleAnomala octiescostata to dandelion,Taraxacum officinale, was demonstrated to be chemically mediated by a mixture ofcis-3-hexenyl acetate, benzaldehyde, phenylacetaldehyde, benzyl alcohol, phenethyl alcohol, phenylacetonitrile, and benzyl benzoate, in the ratio 4:8:14:3:5:19:11. Combination of the synthetic kairomone and sex pheromone (buibuilactone + japonilure, 8:2), significantly increased the total catches ofA. octiescostata. Catches of male (but not female) beetles were significantly higher with the kairomone-pheromone blend than with kairomone alone. The synergistic effect of the kairomone from dandelion on the attractiveness did not significantly differ from that of a food-type lure, anethol, geraniol, and phenethyl propionate (9:0.5:0.5). The latter combined with the synthetic sex pheromone resulted in better attraction of female (but not male)A. octiescostata than the sex pheromone alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02059891
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