Publication Date:
1992-06-26
Description:
B lymphocytes and macrophages express closely related immunoglobulin G (IgG) Fc receptors (Fc gamma RII) that differ only in the structures of their cytoplasmic domains. Because of cell type-specific alternative messenger RNA splicing, B-cell Fc gamma RII contains an insertion of 47 amino acids that participates in determining receptor function in these cells. Transfection of an Fc gamma RII-negative B-cell line with complementary DNA's encoding the two splice products and various receptor mutants indicated that the insertion was responsible for preventing both Fc gamma RII-mediated endocytosis and Fc gamma RII-mediated antigen presentation. The insertion was not required for Fc gamma RII to modulate surface immunoglobulin-triggered B-cell activation. Instead, regulation of activation involved a region of the cytoplasmic domain common to both the lymphocyte and macrophage receptor isoforms. In contrast, the insertion did contribute to the formation of caps in response to receptor cross-linking, consistent with suggestions that the lymphocyte but not macrophage form of the receptor can associate with the detergent-insoluble cytoskeleton.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Amigorena, S -- Bonnerot, C -- Drake, J R -- Choquet, D -- Hunziker, W -- Guillet, J G -- Webster, P -- Sautes, C -- Mellman, I -- Fridman, W H -- New York, N.Y. -- Science. 1992 Jun 26;256(5065):1808-12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratoire d'Immunologie Cellulaire et Clinique, INSERM U 255, Institut Curie, Paris, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1535455" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Antigen-Antibody Complex/metabolism
;
Antigen-Antibody Reactions/genetics/immunology
;
Antigens, CD/genetics/*immunology
;
Antigens, Differentiation/genetics/*immunology
;
B-Lymphocytes/*immunology
;
Calcium/metabolism
;
*DNA-Binding Proteins
;
Dose-Response Relationship, Immunologic
;
Endocytosis/genetics/immunology
;
Humans
;
Immunohistochemistry
;
Lymphocyte Activation/immunology
;
Microscopy, Electron
;
Molecular Sequence Data
;
Receptors, Fc/genetics/*immunology
;
Receptors, IgG
;
Repressor Proteins/pharmacology
;
Transcription Factors/pharmacology
;
Transfection
;
Viral Proteins
;
Viral Regulatory and Accessory Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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