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  • nitrogen  (49)
  • photosynthesis  (41)
  • RFLP
  • Springer  (107)
  • American Physical Society
  • 1990-1994  (107)
  • 1990  (107)
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  • Springer  (107)
  • American Physical Society
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  • 1990-1994  (107)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of paleolimnology 4 (1990), S. 1-22 
    ISSN: 1573-0417
    Keywords: sulfate ; carbon ; nitrogen ; hydrogen ; organic matter ; enrichment factor ; lake sediments ; paleolimnology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract This paper discusses the use of S as a paleolimnological tracer of limnetic sulfate concentration. A positive relationship (p〈0.05) was found between limnetic sulfate and sediment S concentrations for the Great Lakes, English Lakes, and lakes from the Adirondack and Northern New England regions. There is a positive correlation (p〈0.05) between C and S concentration in sediment across all regions studied. The importance of C in affecting S content in sediment was also examined by a series of cores taken at different water depths in Big Moose Lake (Adirondacks). There was a strong relationship between C and S among cores with sediment from deeper water having higher C and S concentrations (r 2=0.99). Sulfur from the shallower cores had greater concentrations of chromium-reducible S (pyrite), while cores from deeper waters had a greater proportion of organic S fractions including C-bonded S and ester sulfates. For assessing historical changes in S accumulation in sediments, enrichment factors were calculated for the PIRLA lakes. Pre-1900 net sediment accumulation rates of S were very similar across all regions. Sulfur enrichment was greatest in Adirondack sediment which had total post-1900 S accumulation of 1.1 to 7.4 times pre-1900 S accumulation. Sediment from Northern New England (NNE) generally had lower S concentration than Adirondack sediments and S enrichment factors ranged from 1.2 to 2.1. Sediment from the Northern Great Lakes States region had similar S concentration and distribution with depth to NNE sediment. In two Northern Florida lakes, sediment showed little variation in S concentration with depth, but in two other lakes from the same region, there was higher S concentration in deeper layers. Lakes which had the greatest enrichment factors also exhibited the most marked changes in C:S ratios. Ratios of C:N showed little variation (10.6 to 26.1) among the PIRLA lakes. A first order model indicated slow decomposition within these organic rich sediments. Elemental concentrations and ratios of sediment from a variety of lakes and reservoirs were complied. Maximum and minimum elemental ratios for all the data were 28 to 8.1 for C:N, 0.81 to 0.11 for C:H, and 675 to 12.5 for C:S, respectively. For the C:S ratios in all regions except the Great Lakes, the maximum ratio was less than 231. Both the maximum and minimum amount of N and H concentration of organic matter is related to biotic processes. The minimum concentration of S is regulated not only by nutrient demands but also by non-assimilatory processes. Sulfur incorporation into sediments is a function of a complex of factors, but limnetic sulfate concentration and organic matter content play a major role in regulating the S content of sediment. Further quantification of S incorporation pathways will aid in the paleolimnological interpretation of sediment S profiles. Such information is also important in assessing how S sediment pools will respond to decreases in limnetic sulfate concentration which may occur with decreases in inputs from acidic deposition.
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  • 2
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    Nutrient cycling in agroecosystems 21 (1990), S. 167-170 
    ISSN: 1573-0867
    Keywords: Mo ; interaction ; soybean ; deficiency ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Recent on-farm liming experiments showed that Mo deficiency in soybean [Glycine max (L.) Merr.] is widespread in northern Alabama. In contrast, a long-term, fertility-rotation experiment in the same area showed no response to Mo during 33 yr when Mo was added bienially to corn [Zea mays L.] in the rotation; however, soybean foliage had the chlorotic appearance of Mo deficiency. The objective of this study was to determine if Mo deficiency was being missed by comparing only two fertilizer treatments. Each rotation-fertilizer treatment plot was split into two, with one-half receiving MO at a 100 g ha−1 foliar rate after seedling emergence, while the other half received none. Yields were increased by Mo in 13 to 16 fertilizer treatments in 1985 and 15 out of 16 in 1986. Leaf-N concentrations and seed weight had comparable increased amounts by the Mo supplement. Without the Mo supplement, there was a response to lime but not to P, K, or a Mo-containing micronutrient mixture; with the Mo supplement, there was no response to liming, but a definite response to P and K (in addition to Mo). The lack of response to Mo when applied to corn in a 2-yr rotation over 33 yr led to the erroneous conclusion that these soils were not Mo deficient for soybean.
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  • 3
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    Nutrient cycling in agroecosystems 22 (1990), S. 71-78 
    ISSN: 1573-0867
    Keywords: Boron ; critical level ; grain ; nitrogen ; nodulation ; nutrition ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A two year field study on the effect of nitrogen N and boron B fertilization on the nodulation, mineral nutrition and grain yield of cowpea was carried out in the Nigerian savanna where fairly widespread B deficiency has been reported. Treatments consisted of four levels of N (0, 15, 30 and 60 kg/ha in 1986 and 0, 15, 30 and 45 kg/ha in 1987) and three levels of B (0, 1.5 and 3.0 kg/ha). Cowpea responded positively though nonsignificantly to N fertilization up to 30 kg N/ha. However, N had no effect on the N, P, K and B content of index leaves. Boron application consistently reduced grain yield. Like N, applied B had no effect on the N, P, K concentration of index leaves but increased B concentration highly significantly (P 〈 0.001). The critical level of B toxicity in index leaves was approximated to be 21pm under field condition. Application of N and B depressed nodulation.
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  • 4
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    Nutrient cycling in agroecosystems 23 (1990), S. 97-103 
    ISSN: 1573-0867
    Keywords: Carbon ; mineralization ; nitrogen ; organic fertilizer ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The C and N mineralization characteristics of two organic N fertilizers were determined in a soil-less incubation system at three temperature regimes. Protox (derived from activated sewage sludge) initially degraded more rapidly by microbial action compared with dried blood. However, dried blood released more CO2-C and inorganic N towards the end of the incubation periods. The rate of microbial degradation increased with temperature. Mineralization characteristics of protein-based N sources are discussed in relation to organic N nutrition of vegetable crops.
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  • 5
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    Nutrient cycling in agroecosystems 23 (1990), S. 105-112 
    ISSN: 1573-0867
    Keywords: Ammonium ; fertigation ; nitrate ; nitrogen ; trickle irrigation ; urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The movement and transformations of ammonium-, urea- and nitrate-N in the wetted volume of soil below the trickle emitter was studied in a field experiment following the fertigation of N as ammonium sulphate, urea and calcium nitrate. Effects on soil pH in the wetted volume were also investigated. During a fertigation cycle (emitter rate 2lh−1) applied ammonium was concentrated in the surface 10 cm of soil immediately below the emitter and little lateral movement occurred. In contrast, because of their greater mobility in the soil, fertigated urea and nitrate were more evenly distributed down the soil profile below the emitter and had moved laterally in the profile to 15 cm radius from the emitter. The conversion of applied N to nitrate-N was more rapid when urea rather than ammonium-N was applied suggesting that the accumulation of large amounts of ammonium below the emitter in the ammonium sulphate treatment probably retarded nitrification. Following their conversion to nitrate-N, both fertigated ammonium sulphate and urea caused acidification in the wetted soil volume. Acidification was confined to the surface 20 cm of soil in the ammonium sulphate treatment, however because of its greater mobility, fertigation with urea (2lh−1) resulted in acidification occurring down to a depth of 40 cm. Such subsoil acidity is likely to be very difficult to ameliorate. Increasing the trickle discharge rate from 2lh−1 to 4lh−1 reduced the downward movement of urea and encouraged its lateral spread in the surface soil. As a consequence, acidification was confined to the surface (0–20 cm) soil.
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  • 6
    ISSN: 1573-5028
    Keywords: retrotransposon ; Pisum ; segregation ; PCR ; RFLP ; mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA sequence between two legumin genes in Pisum is a member of the copia-like class of retrotransposons and represents one member of a polymorphic and heterogeneous dispersed repeated sequence family in Pisum. This sequence can be exploited in genetic studies either by RFLP analysis where several markers can be scored together, or the segregation of individual elements can be followed after PCR amplification of specific members.
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  • 7
    ISSN: 1573-5028
    Keywords: cDNA ; cyanogenesis ; β-glucosidase ; RFLP ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Li locus in white clover controls the presence of cyanogenic β-glucosidase (linamarase) activity in leaf tissue, such that plants homozygous for the ‘null’ allele (li) have no linamarase activity in this tissue. The isolation of a cDNA clone from linamarase mRNA is described. The cDNA clone is used to further characterise alleles of the Li locus. Northern blot analysis shows that plants homozygous for the ‘null’ allele (li li) produce very reduced levels of mRNA which hybridises to the cDNA. Heterozygous plants (Li li), which have intermediate levels of enzyme activity, produce intermediate levels of mRNA. Southern blot analysis of Hind III digested genomic DNA shows that the white clover genome contains three genes with homology to the linamarase cDNA and that at least two of these genes segregate independently. Analysis of the cosegregation of linamarase activity and the presence of genomic restriction fragments identifies the genomic sequence specifying linamarase structure and indicates either a structural or cis acting control function of the Li locus.
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  • 8
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    Theoretical and applied genetics 79 (1990), S. 577-582 
    ISSN: 1432-2242
    Keywords: Genetic marker ; RFLP ; Quantitative traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Digestion of genomic DNA with the restriction endonuclease Avail disclosed a probable insertion deletion of approximately 200 base pairs (bp) near the prolactin gene. Two alleles were apparent as three distinct hybridization patterns. These alleles were statistically associated with quantitative trait loci among sons of one elite Holstein sire family. The favorable genotype was correlated with the presence of a 1.15-kb hybridization band inherited from the sire when genomic DNA was probed with a full-length cDNA for prolactin. Pedigree estimates of genetic merit among genotypes were similar, differing by only 19.3 kg for milk in ancestor merit. Comparisons of genetic estimates for quantitative yield traits in offspring of this heterozygous sire showed significant (P〈0.05) differences between homozygous genotypes for predicted difference milk (PDM), predicted difference dollars (PD$), cheese yield dollars, and protein dollars. The estimated differences between homozygous genotypes for USDA Transmitting Abilities of PDM, PD$, Cheese Yield $ and Protein $ were 282.93 kg, $74.35, $48.58 and $53.67, respectively. However, the estimated breeding values from progeny ranged over 900 kg in transmitting ability for milk. Frequency of the favorable marker allele was estimated to be 0.231 in the elite cow population used as dams of sons. These results demonstrate the potential of molecular biological techniques to discriminate between individuals within a family and to predict breeding values for selection schemes.
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  • 9
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    Theoretical and applied genetics 80 (1990), S. 673-679 
    ISSN: 1432-2242
    Keywords: Rice ; Tissue culture ; Somaclonal variation ; RFLP ; Methylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Regenerants of rice were examined by RFLP analysis to determine the occurrence and extent of somaclonal variation. DNA polymorphisms were observed both among plants regenerated from different callus cultures as well as among sibling plants derived from a single callus. Regardless of the basal medium, a higher degree of genetic instability was found among plants regenerated from callus cultures maintained for longer incubation periods (67 days) than among those from shorter incubation periods (28 days). Detailed analysis showed that in several regenerants, there was a close correlation among those plants exhibiting DNA rearrangements and those with apparent methylation changes. Such alterations were observed with both structural and housekeeping genes.
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  • 10
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    Theoretical and applied genetics 80 (1990), S. 680-686 
    ISSN: 1432-2242
    Keywords: Retrotransposon ; Zea ; RFLP ; Bs1 ; Transposable element
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty-eight accessions from Zea and 20 accessions from related genera were probed for the presence of Bs1, a retrotransposon originally found in maize. All maize and teosinte plants tested show the presence of Bs1 in one to five densely hybridizing bands. The mean copy numbers of Bs1 elements among the maize and teosinte accessions were similar: 2.92 and 3.25, respectively, with no large differences between any subgroups. Most exotic maize samples exhibited two common bands of 7.8 kb and 4.7 kb. Section Zea teosintes (but not teosintes of section Luxuriantes) also show the presence of a common band of the same size as the smaller common band in maize. At reduced stringency, Tripsacum dactyloides exhibited a single hybridizing band at 6.9 kb. Results argue for the evolution of maize from a mexicana or parviglumis teosinte, and the evolution of the Bs1 element within the tribe Andropogoneae. Additionally, recombinant inbred lines were probed for the presence of Bs1, in order to map the chromosomal locations of Bs1 elements in four different maize lines. Two of the recombinant inbred parental lines had an element (Bs1-F) on chromosome 5, while the other two lines had an element (Bs1-S) on chromosome 8. Restriction site polymorphisms have apparently arisen in the vicinity of Bs1-S since its insertion. Segregation analysis of other lines was also performed; the data indicate that Bs1 has the distribution expected of a transposable element, different locations in different lines, and not that of a fixed gene locus. However, the common bands in the Zea mays lines and the recombinant inbred data imply that Bs1 is not highly mobile.
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  • 11
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    Theoretical and applied genetics 79 (1990), S. 360-368 
    ISSN: 1432-2242
    Keywords: RFLP ; Solanum ; Phylogenetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Phylogenetic relationships between 14 wild and 3 cultivatedSolanum species, including the European potato,Solanum tuberosum ssp.tuberosum, were inferred using DNA restriction fragment length polymorphisms (RFLPs) as discriminating characters. Phenetic trees were obtained based on distance matrices as well as on parsimony methods, which were not significantly altered either by the computational method used, or by the individual plant genotypes or RFLP markers chosen for the analysis. The reliability of the tree topologies was assessed and, as expected, it increased with the number of polymorphic restriction fragments scored. The individual genotypes within each species, the different species themselves, and the main branches of the trees were clearly separated from each other. The least reliable parts in the trees were the positions of closely related species within the main clusters.S. tuberosum spp.tuberosum formed one group closely related withS. tuberosum spp.andigena,S. stenotomum, andS. canasense. This was well separated from a second group formed byS. sparsipilum,berthaultii,kurtzianum,gourlayi,vernei,spegazzinii,chacoense, andmegistacrolobum. Two further branches were formed byS. demissum andS. acaule, and byS. polyadenium,S. pinnatisectum,S. stoloniferum, andS. etuberosum. The phenetic trees presented here supported the description of relationships amongSolanum species based on biosystematic studies, with the exception of the placement ofS. chacoense andS. megistacrolobum.
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  • 12
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    Theoretical and applied genetics 79 (1990), S. 705-712 
    ISSN: 1432-2242
    Keywords: Erysiphe graminis ; Genetic map ; Repetitive ; DNA ; RFLP ; Virulence genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genome organization of the biotrophic barley powdery mildew fungus was studied using restriction fragment length polymorphism (RFLP). Genomic DNA clones containing either low-or multiple-copy sequences appeared to be the best RFLP markers, as they frequently revealed polymorphisms that could be readily detected. A total of 31 loci were identified using 11 genomic DNA clones as probes. Linkage analysis of the 31 RFLP loci and five virulence loci resulted in the construction of seven groups of linked loci. Two of these contained both RFLP markers and virulence genes. RFLP markers were found to be very efficient in characterizing mildew isolates, as only three markers were necessary to differentiate 28 isolates. The DNA of the barley powdery mildew fungus appeared to contain a considerable number of repetitive sequences dispersed throughout the genome.
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  • 13
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    Theoretical and applied genetics 79 (1990), S. 763-768 
    ISSN: 1432-2242
    Keywords: DNA fingerprint ; M13 probe ; RFLP ; Paternity analysis ; Apple
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Analysis of minisatellite DNA sequences, yielding so called DNA “fingerprints”, has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 “fingerprint” probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.
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  • 14
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    Theoretical and applied genetics 80 (1990), S. 437-448 
    ISSN: 1432-2242
    Keywords: Lycopersicon ; Phylogeny ; RFLP ; Genetic variation ; Breeding system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Forty single-copy, nuclear probes of known chromosomal position were used to examine restriction fragment length polymorphism in the tomato genus Lycopersion. The probes were from three libraries: one cDNA, and two genomic libraries ⦓ne genomic made with EcoRI and the other with PstI. Total DNA from 156 plants representing eight species was cut with five different restriction enzymes and scored in 198 probe-enzyme combinations. Genetic distances between accessions (populations) and species were calculated from the resultant restriction patterns and proportion of shared bands. Accessions belonging to the same species largely clustered together, confirming their current classification. However, one mountain accession, classified as L. peruvianum var. humifusum (LA2150), was sufficiently distinct from the other accessions of L. peruvianum that it may qualify as a separate species L. esculentum and L. pimpinellifolium were the least clearly differentiated, possibly reflecting introgressive hybridization, known to have been promoted by man in recent history. Dendrograms constructed from cDNA versus genomic clones were nearly identical in their general grouping of species. The dendrograms revealed two major dichotomies in the genus: one corresponding to mating behavior [self-compatible (SC) versus self-incompatible (SI) species] and the other corresponding to fruit color (red versus green-fruited species). The ratio of withinversus between-accession diversity was much lower for SC species, indicating that most of the diversity within these species exists between populations, rather than within populations. Overall, the amount of genetic variation in the SI species far exceeded that found in SC species. This result is exemplified by the fact that more genetic variation could be found within a single accession of one of the SI species (e.g., L. peruvianum) than among all accessions tested of any one of the SC species (e.g., L. esculentum or L. pimpinellifolium). Results from this study are discussed in relationship to germ plasm collection/utilization and with regard to the use of RFLPs in tomato breeding and genetics.
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  • 15
    ISSN: 1432-2242
    Keywords: Tomato ; RFLP ; rDNA ; Tobacco mosaic virus ; Introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We studied rDNA restriction fragment length polymorphism between two tomato lines used for F1 hybrid seed production: line A, containing the Tm-1 gene responsible for tobacco mosaic virus tolerance introgressed from the wild species Lycopersicon hirsutum, and line B, a tobacco mosaic virus sensitive line. Hybridization patterns led to distinct rDNA maps with two size classes, 10.4 and 10.7 kb, in line A and a single, 8.9-kb class in line B. Size differences were located in the intergenie sequence (IGS). A highly specific 54-bp TaqI fragment was cloned from the line A IGS and used in dot blot experiments to probe total DNA from line A, line B, and their F1 hybrid. It proved capable of discriminating B from A and the hybrid. This probe could thus serve to screen inbreds in commercial seed lots where line A is used as male. This fragment showed 80–90% sequence homology with the 53-bp subrepeats previously characterized in a region of the tomato IGS close to the 25S rRNA gene. Preliminary comparison of rDNA in line A and several wild related species indicated that the L. hirsutum H2 genotype was the closest to line A. rDNA variations between line A and this wild genotype could be explained by recombination during the introgression process involving numerous backcrosses or by an important intraspecific polymorphism. Our results strongly suggest that Tm-1 and the rDNA were introgressed together into tomato from L. hirsutum through linkage drag.
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  • 16
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    Theoretical and applied genetics 79 (1990), S. 465-469 
    ISSN: 1432-2242
    Keywords: Soybean ; RFLP ; Quantitative trait loci ; Germination ; Hard seededness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hard seededness in soybean [Glycine max (L.) Merr.] is a quantitative trait that affects the germination rate, viability, and quality of stored seeds. We have used 72 restriction fragment length polymorphisms (RFLPs) to identify genomic regions containing quantitative trait loci (QTL) affecting hard seededness in a segregating population from a G. max by a Glycine soja (Sieb. & Zucc.) cross. Five independent RFLP markers were found to be associated with variation in the hard-seeded trait. These markers and the epistatic interactions between them explain 71% of the variation for hard seededness. A genomic region associated with the i locus accounted for 32% of the variation in this segregating population. This study illustrates one approach to physiological genetic studies in plants.
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  • 17
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    Theoretical and applied genetics 80 (1990), S. 385-389 
    ISSN: 1432-2242
    Keywords: RFLP ; Tomato ; Probe source ; Probe length ; Restriction enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Since the construction and use of RFLP genetic maps depends on the ability of cloned sequences to detect polymorphism, we have attempted to determine conditions under which maximum levels of polymorphism can be detected. Forty cloned nuclear sequences from three different libraries (cDNA, EcoRI genomic, and Pstl genomic) were hybridized to total DNA from 149 plants representing eight species of the tomato genus Lycopersicon. Five different restriction enzymes were employed in this study. We examined the relationship between polymorphism (number of restriction patterns) and clone size, restriction enzyme, size of hybridizing restriction fragments, and clone source (library). We found no relationship between clone size (ranging from 0.4 to 5.3 kb) and polymorphism. There was a strong positive relationship (r 2 = 0.79) between polymorphism and the average size of the fragments produced by each restriction enzyme. cDNA clones hybridized to larger fragments compared to genomic clones. cDNAs also detected significantly more polymorphism (approximately 25% more) than genomic clones — possibly indicating high levels of sequence variability in introns and/or areas flanking coding regions.
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  • 18
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    Theoretical and applied genetics 79 (1990), S. 81-88 
    ISSN: 1432-2242
    Keywords: Cultivated rice ; Wild rice ; RFLP ; Ribosomal RNA genes ; Hybridisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism of the rDNA spacer was studied in the genus Oryza using a cloned rice rDNA probe. One-hundred-five accessions, including 58 cultivated rice and 47 wild species with various genome types, were analysed. Seven size classes differing from one another by an “increment” of ca. 300 bp were observed amongst the Asiatic cultivated rice of the species O. sativa. A general tendency from a smaller spacer in the Japonica subtypes to longer ones in Indica is observed. Classification as Japonica or Indica on the basis of rDNA pattern generally agrees with classification based on isozyme patterns. In contrast, African rice of the species O. glaberrima does not display any rDNA size variation. When wild species are considered, extensive variation is observed, but the fragment sizes do not fall into regularly increasing size classes except for O. rufipogon and O. longistaminata. The variation is greater in these species than in the cultivated ones.
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  • 19
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    Theoretical and applied genetics 79 (1990), S. 235-240 
    ISSN: 1432-2242
    Keywords: Glycine spp. ; RFLP ; Taxonomy ; Wild perennial species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total DNA from callus tissue of 28 accessions representing seven wild perennial Glycine species was compared using recombinant genomic probes derived from G. max, the soybean. Using two probes, we show that this molecular approach both confirms and extends the model for the taxonomic relationships between the species derived from morphological and cytogenetic data, and that it provides clear evidence that RFLP analysis of genomic sequences has the potential for revealing the derivation of the member species of the wild perennial Glycine taxon. Although, in this preliminary report, the sample size for each species is small, it is clear that the greatest between-accession variation occurs in G. tabacina (B2B2) and G. clandestine (A1A1), suggesting that these may be the taxa from which further speciation occurred in the subgenus.
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  • 20
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    Theoretical and applied genetics 80 (1990), S. 545-551 
    ISSN: 1432-2242
    Keywords: Somatic fusion ; Solanum brevidens ; RFLP ; Solanum tuberosum ; DNA introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism (RFLP) markers were used to distinguish the chromosomes of Solanum brevidens from those of potato (S. tuberosum) in a fertile somatic hybrid. The hybrid had markers that account for all 24 chromosome arms from each parent, indicating that the hybrid contained at least one copy of each chromosome from each parent. The markers were then used to follow segregation of chromosomes in sexual progeny that resulted from a cross of the somatic hybrid with the potato cultivar ‘Katahdin’. Approximately 10% of the sexual progeny lacked one or more of the markers specific to S. brevidens. No one chromosome or marker appeared to be lost preferentially. This infrequent absence of a chromosome marker derived from the wild parent could be explained by intergenomic pairing and recombination. The loss of a marker band for chromosome 8, coupled with the retention of two flanking markers, suggested that a small region of DNA was deleted during regeneration of the somatic hybrid. These results show the value of RFLP analysis when applied to somatic hybrids and their progeny. Clearly, RFLPs will be useful for following the DNA from wild species during its introgression into potato cultivars.
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  • 21
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    Journal of applied phycology 2 (1990), S. 293-296 
    ISSN: 1573-5176
    Keywords: heavy metal ; photosynthesis ; periphyton ; tolerance ; Cyanophyceae ; genetic adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study was made of the tolerance to Cu of 11 strains of Cyanophyceae and 7 strains of eukaryotes. These had all been tested within 6 months after isolation for their photosynthetic activity when exposed to Cu (Takamuraet al., 1989) and had repeatedly been subcultured in the medium without Cu for 2 years. Photosynthetic measurements were made in two ways: precultured in medium without Cu or precultured (for one subculture) in medium containing Cu (645 μg 1−1). The results were compared with those obtained within 6 months of isolation. The tolerance of the eukaryotes did not change significantly in any case, but most strains of Cyanophyceae lost their tolerance to Cu within a few subcultures in medium without Cu; however tolerance recovered following one subculture in medium containing an intermediate level of Cu. This rapid adaptation cannot be explained by a constitutive mutation.
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  • 22
    ISSN: 1573-5176
    Keywords: Cystoseira barbata ; photosynthesis ; light ; temperature ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The net photosynthesis of the Mediterranean brown seaweedCystoseira barbata f.repens is measured according to irradiance, temperature and salinity. There is not only, a good utilization of low light intensities (light-shade adaptation), but also a specific ability to use a broad range of irradiance, which corresponds in the photosynthesis-irradiance curves to a high initial slope and an extended light saturation level from 300 to 1500 μmol photon m−2 s−1; only very high irradiances induce photoinhibition. Maximum net photosynthesis occurred at temperatures ranging from 20 °C to 30 °C. The alga tolerates not only a low level of salinity, but also a slight increase in salinity; however, at more than 47.5 g 1−1 NaCl, oxygen exchange is significantly reduced. Light, temperature and salinity requirements are discussed, taking into account ecological considerations. Yields and quality of alginic acid are presented according to the irradiance and yearly evolutionin situ in order to aid future cultivation of this species.
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  • 23
    ISSN: 1573-5176
    Keywords: Gracilaria ; strain selection ; growth ; photosynthesis ; rubisco ; agar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain selection procedure using Gracilaria verrucosa gametophytic sporelings was found to be an efficient tool for the improvement of Gracilaria strains. Two strains, C-2 and A-18, which were isolated and grown clonally, showed higher growth rates under high and low temperature conditions, respectively, than the local Gracilaria conferta. Growth rate, photosynthesis and chlorophyll, which were measured under different temperature and photon flux densities, demonstrated an overall advantage of the selected strains over the wild type strains of both G. verrucosa and G. conferta. Growth rates were also generally in positive correlation with the carboxylase activity of Rubisco. The G. verrucosa wild type also had a 40% higher agar content than G. conferta. The selected strains thus showed higher potential for outdoor cultivation than local wild type populations.
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  • 24
    ISSN: 1573-5036
    Keywords: disturbance ; fertilizer ; nitrogen ; nutrient enrichment ; phosphorus ; secondary succession ; species richness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of annual additions of mineral N and P (100 kg ha−1) on plant species composition and annual aboveground net primary production (ANPP) were investigated during the first three years following disturbance in a semi-arid ecosystem. Additions of N reduced richness of perennial plant species during years 2 and 3, while P reduced the number of perennial species only in year 3. From year 1 to year 2, annual and biennial species richness declined in all treatments while ANPP of annual species increased greatly. Added N increased ANPP of annual species while it decreased ANPP of most perennial species relative to the unfertilized control treatment. Community similarities were higher for the control and native vegetation than for other pairs of treatments using both species presence and plant production data. Nitrogen additions have retarded but not completely arrested secondary succession in this system.
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  • 25
    ISSN: 1573-5036
    Keywords: eucalypt ; foliar analysis ; legume ; nitrogen ; phosphorus ; plant nutrients ; plant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The sensitivity of tissue nutrient concentrations to changes in plant age and the supply of P and N was compared between leaves and associated twigs in two forest species. In a young regrowth stand, tissues were sampled on three occasions from the mid-crown position of karri (Eucalyptus diversicolor F. Muell.) and Bossiaea laidlawiana Tovey and Morris, a major understorey legume. Leaves and twigs were also sampled from young plants of B. laidlawiana growing in a mature eucalypt stand to which P treatments had been applied. Nitrogen application increased N concentrations in twigs of karri and B. laidlawiana, but not in leaves. Phosphorus application increased P concentrations in both leaves and twigs of karri but the average increases were proportionally greater in twigs (65%) than in leaves (36%). Over the sampling period, P concentrations in leaves declined, while those in twigs were relatively stable. In B. laidlawiana, P supply also had a larger effect on P concentrations in twigs than in leaves. Addition of 200 kg P ha−1 increased average P concentrations in twigs by 109% in the regrowth stand and by 215% in the mature stand while the corresponding increases in leaves were only 11% and 27%. Concentrations of other nutrients in both species were also affected by N and P application, the most notable being a decline in the concentrations of the minor nutrients, Zn and Cu, with increased P supply. The increased N concentrations in twigs of karri, and the increased P concentrations in tissues of both species, were associated with responses of karri to added N and P, and of B. laidlawiana to added P. This indicates that tissue concentrations of N and P were generally below critical concentrations where N and P were not applied. The results show that for these species twigs may be a better tissue than leaves for diagnosing deficiencies or predicting N and P requirements. The ratio of P concentrations in twigs to P concentrations in leaves also increased with added P. It is suggested that this ratio may be a useful index if it reduces the variability caused by sampling position within the crown or genetic differences between plants.
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  • 26
    ISSN: 1573-8469
    Keywords: Canopy enclosure ; stomatal conductance ; light response curve ; light use efficiency ; photosynthesis ; Solanum tuberosum L. ; transpiration ; water use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Aardappelplanten (Solanum tuberosum L.) cv.Saturna werden onderworpen aan stress als gevolg vanVerticillium dahliae en droogte. In vroege stadia van de groei werden stomataire geleiding, transpiratie en netto fotosynthese bij lichtverzadiging (PAR〉300 W m−2) gemeten aan individuele bladeren en met een mobiel instrumentarium met behulp van gewaskappen. Er werden geen significante verschillen gevonden in de waarden van de stomataire geleiding en de gasuitwisslingskarakteristieken als gevolg vanV. dahliae-besmetting tot een maand na opkomst. Daarna leidde infectie metV. dahliae tot een afname van de stomataire geleiding, transpiratie en netto fotosynthese, speciaal bij oudere bladeren en bij planten die meer aan zonlicht waren blootgesteld. Soms vertoondeV. dahliae interactie met droogte en bleken beide effecten minder dan optelbaar. De hoge waarden van de variatiecoëfficiënten maakten een groot aantal metingen per behandeling noodzakelijk; dit was vooral het geval bij metV. dahliae geïnfecteerde planten hetgeen aantoont datV. dahliae vooral in het begin van de groei niet alle bladeren in gelijke mate aantast. Door de matigende invloed van de integratie van alle bladlagen en mogelijk doordat de bovenste bladeren werden gestimuleerd, werd de totale gewasfotosynthese in mindere mate beïnvloed doorV. dahliae dan de individuele bladfotosynthese. De bovenste niet geïnfecteerde bladeren bleken verantwoordelijk voor het grootste gedeelte van de gewas-fotosynthese. De resultaten tonen aan, dat volgend op een infectie metV. dahliae, de fotosynthese reeds in een vroeg stadium van de groei wordt verminderd als een gevolg van droogtestress in de bladeren.
    Notes: Abstract Potato (Solanum tuberosum L.) plants cv.Saturna were subjected to infection withVerticillium dahliae and drought stress. At the early stages of growth, stomatal conductance, transpiration and net photosynthesis were measured at light saturation (PAR〉300 m−2) on individual leaves and with mobile field equipment with the aid of field enclosures. No significant changes in stomatal conductance and gas exchange characteristics occurred as a result ofV. dahliae instomatal conductance, transpiration and and photosynthetic rates, especially on older leaves and on plants exposed to direct sunlight for a longer period of time. In combination with drought,V. dahliae only occasionally showed interaction; their effects being less than additive. High values of coefficients of variatoon necessitated a high number of measurements per treatment; the more so in the inoculated plants which shows thatV. dahliae seems to affect certain leaves while not affecting others early in growth. Crop photosynthesis was less reduced byV. dahliae than individual leaf photosynthesis due to the levelling effect of integration over the whole canopy and possibly through a stimulation of the top leaves. The upper non-affected leaves are responsible for the bulk of photosynthetic crop activity. The results indicate that following an infection withV. dahliae photosynthesis is reduced early in growth as a result of drought stress in the leaves.
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  • 27
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    Molecular genetics and genomics 220 (1990), S. 489-491 
    ISSN: 1617-4623
    Keywords: Daucus ; Carrot ; Plastid DNA ; RFLP ; Paternal plastid inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The plastid DNAs of the species Daucus carota (ssp. sativus, libanotifolia, gingidium), D. maximus and D. muricatus were compared by restriction enzyme analysis. A number of restriction fragment length polymorphisms (RFLPs) were observed. As expected from taxonomic data the degree of plastid DNA homology between D. carota and D. maximus is significantly higher (97%) than between D. carota and D. muricatus (70%). On the basis of RFLPs of plastid DNA the mode of plastid inheritance in interspecific crosses between D. muricatus and D. c. sativus was analysed. The results clearly indicate paternal plastid inheritance. Thus Daucus is the second genus among angiosperms transmitting predominantly male plastids.
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  • 28
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    Molecular genetics and genomics 223 (1990), S. 324-328 
    ISSN: 1617-4623
    Keywords: Rice ; Protoplast ; RFLP ; Methylation ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To determine whether regeneration of rice plants from protoplast culture induces DNA polymorphisms, progeny plants from direct regenerants of such cultures were examined for restriction fragment length polymorphisms (RFLP analysis). Significantly increased levels of DNA polymorphism were found compared with those in non-tissue culture control plants. Analysis with gene sequences representative of different functional domains, revealed that such polymorphisms are apparently widespread and not associated with any particular region. Analysis by comparative digestion with both methylation-sensitive and insensitive restriction enzymes revealed that methylation changes cannot be regarded as a major factor in the induction of these DNA polymorphisms.
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  • 29
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    Plant and soil 123 (1990), S. 67-71 
    ISSN: 1573-5036
    Keywords: Dalbergia sissoo ; fertilization ; nitrification ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of added ammonium, phosphorus, potassium, and gypsum on net nitrogen mineralization was studied in soil beneath a six-year-old plantation of the N2-fixing tree Dalbergia sissoo in Pakistan. Soil with and without amendments was placed in polyethylene bags and incubated, buried in the soil, for 30 days. After that time the soil was analyzed and net ammonium and nitrate production and net nitrogen mineralization were calculated. The addition of ammonium stimulated nitrification indicating that the process was substrate limited. The inhibition of nitrification by Nitrapyrin showed that the process is autotrophic in these soils. Gypsum addition lowered soil pH from 8.0 to 7.2 and significantly stimulated ammonification, nitrification and net nitrogen mineralization. The addition of potassium more than tripled the soil K:Na ratio. Net ammonium and nitrate production and net nitrogen mineralization all increased in this treatment. The addition of phosphorus had no significant effect on soil nitrogen dynamics.
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  • 30
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    Plant and soil 127 (1990), S. 213-218 
    ISSN: 1573-5036
    Keywords: dry matter yield ; nickel ; nitrogen ; nutrient concentration ; wheat roots ; wheat tops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A glasshouse experiment was conducted to study the effect of Ni on the growth and nutrients concentration in wheat (Triticum aestivum Cv. WH 291) in the presence and absence of applied N as urea. Responses to N application were observed up to 120 μg N g−1 soil. No response to Ni was observed in the dry matter yield of wheat tops (leaves + stem) in the absence of applied N while in the presence of applied N, significant yield increases were obtained at 12.5μg Ni g−1 soil. Nickel was not toxic to wheat up to 50μg Ni g−1 soil in the presence of 120μg N g−1 soil. Nitrogen and Ni concentration in wheat tops and roots increased with increasing levels of applied N and Ni, respectively. Applied Ni had an antagonistic effect on N concentration. Similarly, N reduced the Ni concentration in the wheat tissues. Positive growth responses to Ni were associated with 22 and 15μg Ni g−1 in wheat tops, in the presence of applied N at 60 and 120μg N g−1 soil, while Ni toxicity was associated with 63, 92.5 and 112.5μg Ni g−1 in wheat tops, in the absence and presence of applied N at 60 and 120μg N g−1 soil, respectively.
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  • 31
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    Plant and soil 128 (1990), S. 21-30 
    ISSN: 1573-5036
    Keywords: allocation ; clearcut ; mineralization ; nitrogen ; prescribed fire
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Past and current work on biological processes related to nitrogen fluxes and cycling in natural and disturbed Mediterranean forest sites are discussed. In natural conditions, the main point reviewed is mineral nitrogen availability in the soil, and particularly the process of mineralization (ammonification, nitrification) in the field as well as nitrogen uptake by Pinus pinea. Some aspects of nitrogen translocation within the trees are also considered. Perturbation of the nitrogen status, and especially nitrogen mineralization, as a result of manipulation of forest sites are discussed by comparing both a holm oak coppice with a clearcut and a Pinus halepensis woodland before and after prescribed fire.
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  • 32
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    Plant and soil 125 (1990), S. 19-27 
    ISSN: 1573-5036
    Keywords: amino acid ; deficiency ; essentiality ; malate ; micronutrient ; nickel ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Barley (Hordeum vulgare L. cv. ‘Onda’) plants were grown in nutrient solutions supplied either 0 (no Ni added), 0.6, or 1.0 μM NiSO4. Plants supplied 0 μM Ni developed Ni deficiency symptoms; Ni deficiency resulted in the disruption of nitrogen metabolism, and affected the concentration of malate and various inorganic anions in roots, shoots, and grain of barley. The concentrations of 10 of the 11 soluble amino acids determined were 50–200% higher in 30-day-old shoots of plants supplied inadequate Ni levels than in shoots of Ni-supplied plants. The total concentration of all amino acids determined was higher in roots and grain of Ni-deficient plants. Concentrations of NO3 - and Cl- were also higher in Ni-deficient barley shoots than in Ni-sufficient barley shoots. In contrast, the concentration of alanine in shoots of Ni-deficient barley was reduced to one-third of the concentration in Ni-sufficient plants. The shoot concentrations of malate and SO4 2- were also depressed under Ni-deficient conditions. Total nitrogen concentration in grain, but not in shoots, of Ni-deficient plants was significantly increased over that found in Ni-adequate plants. Nickel deficiency results in marked disruptions of N metabolism, malate and amino acid concentrations in barley. These results are discussed in view of the possible roles of Ni in plants.
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  • 33
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    Plant and soil 125 (1990), S. 119-128 
    ISSN: 1573-5036
    Keywords: ammonia ; application method ; application rate ; environment ; grassland ; nitrogen ; slurry ; volatilization ; wind speed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three experiments were conducted to examine the influence of slurry application rate, wind speed and applying slurry in narrow bands on ammonia (NH3) volatilization from cattle slurry surface-applied to grassland. The experiments were conducted in the field using a system of small wind tunnels to measure NH3 loss. There was an inverse relationship between slurry application rate and the proportion of NH4 +-N volatilized. From slurry applied at 20, 40, 60, 80, 100 and 120 m3 ha-1, the respective proportions of NH4 +-N lost by NH3 volatization in 6 days were 60, 56, 49, 40, 44 and 44%. The negative relationship was most pronounced in the first 24 hours after application when 57–77% of the total loss for 6 days occurred. Wind speed had a positive effect on NH3 volatilization, although the effect was small in relation to the total loss; increasing the wind speed from 0.5 to 3.0 m s-1 increased the total 5 day loss by a factor of 0.29. The effect of wind speed was also most pronounced in the first 24 hours when much of the NH3 loss took place. The effect of reducing the surface area of the applied slurry was examined by comparing NH3 volatilization from slurry broadcast across plots with that applied in narrow bands. Although the rate of NH3 volatilization was considerably smaller from the banded application immediately after the slurry was applied, the difference between the treatments progressively narrowed until 2 days after application, after which a higher rate was maintained from the banded slurry. After 5 days the total loss from the banded application was 83% of that from broadcast slurry.
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  • 34
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    Plant and soil 125 (1990), S. 109-117 
    ISSN: 1573-5036
    Keywords: ammonia ; environment ; grassland ; mechanical separation ; nitrogen ; slurry ; volatilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three experiments were conducted using a system of small wind tunnels to measure ammonia (NH3) volatilization from cattle slurry after surface application to land. In each experiment slurry was applied at a rate equivalent to 80 m3 ha-1, providing the equivalent of approximately 100 kg NH4 +-N ha-1. The first experiment compared NH3 volatilization from the liquid fraction obtained by mechanical separation of slurry with that from unseparated slurry. The total NH3 loss over six days from unseparated and separated slurry were very similar, being 38 and 35% respectively of the NH4 +-N applied. For the first five hours, the rate of NH3 loss was higher from the unseparated slurry, thereafter it was consistently lower. In the second experiment, slurry was ponded in a tray to examine whether impeded infiltration or changes in the NH4 + concentration or overall pH of the slurry influenced the rapid decline in rate soon after application that is characteristic of NH3 volatilization from animal slurries applied to land. It appeared, however, that other factors such as resistance to diffusion within the slurry and/or at the slurry surface were mostly responsible for the rapid decline in rate. In the third experiment, in which NH3 volatilization was measured from slurry applied to grassland or bare soil, the total loss from slurry applied to grassland was approximately 1.5 times that from slurry applied to bare soil.
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  • 35
    ISSN: 1573-5079
    Keywords: cytochrome c ; photosynthesis ; photosynthetic bacteria ; electron transport ; Chloroflexus aurantiacus ; green bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The membrane-bound photooxidizable cytochrome c-554 from Chloroflexus aurantiacus has been purified. The purified protein runs as a single heme staining band on SDS-PAGE with an apparent molecular mass of 43 000 daltons. An extinction coefficient of 28 ± 1 mM−1 cm−1 per heme at 554 nm was found for the dithionite-reduced protein. The potentiometric titration of the hemes takes place over an extended range, showing clearly that the protein does not contain a single heme in a well-defined site. The titration can be fit to a Nernst curve with midpoint potentials at 0, +120, +220 and +300 mV vs the standard hydrogen electrode. Pyridine hemochrome analysis combined with a Lowry protein assay and the SDS-PAGE molecular weight indicates that there are a minimum of three, and probably four hemes per peptide. Amino acid analysis shows 5 histidine residues and 29% hydrophobic residues in the protein. This cytochrome appears to be functionally similar to the bound cytochrome from Rhodopseudomonas viridis. Both cytochrome c-554 from C. aurantiacus and the four-heme cytochrome c-558-553 from R. viridis appear to act as direct electron donors to the special bacteriochlorophyll pair of the photosynthetic reaction center. They have a similar content of hydrophobic amino acids, but differ in isoelectric point, thermodynamic characteristics, spectral properties, and in their ability to be photooxidized at low temperature.
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  • 36
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    Photosynthesis research 23 (1990), S. 67-72 
    ISSN: 1573-5079
    Keywords: leaf size ; light ; photosynthesis ; shading ; soybeans ; specific leaf weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study investigated the basis of the negative relationship between leaf size and photosynthetic rate per unit of area among five cultivars of soybeans. Exposure of developing mainstem leaves to light, and sizes and light saturated photosynthesis rates of those leaves at maturity were compared in cultivars grown in field plots for two years at Beltsville, Maryland, USA. Plants were grown both in stands at 2.5 cm by 1 m spacing and as isolated plants. While cultivar differences in leaf size were large and consistent in both planting arrangements, significant cultivar differences in light saturated photosynthetic rates were found only in plants grown in stands. Similarly, leaf size was significantly correlated with specific leaf weight only for plants grown in stands. The mainstem apex and developing mainstem leaves experienced more severe shading in large-leaved cultivars than in small-leaved cultivars when plants were grown in stands. Thus, cultivar differences in photosynthetic capacity were probably a consequence of differences in the exposure of developing leaves to light.
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  • 37
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    Photosynthesis research 23 (1990), S. 313-318 
    ISSN: 1573-5079
    Keywords: thylakoid membranes ; electron transfer ; photoacoustic spectroscopy ; energy storage ; photosynthesis ; plastoquinone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthetic energy storage yield of uncoupled thylakoid membranes was monitored by photoacoustic spectroscopy at various measuring beam intensities. The energy storage rate as evaluated by the half-saturation measuring beam intensity (i50) was inhibited by 3-(3,4-dichlorophenyl)-1,1 dimethylurea, by heat inactivation or by artificial electron acceptors specific for photosystem I or photosystem II; and was activated by electron donors to photosystem I. The reactions involving both photosystems were all characterized by a similar maximal energy storage yield of 16±2 percent. The data could be interpreted if we assumed that the energy storage elicited by the photosystems at 35 Hz is detected at the level of the plastoquinone pool.
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  • 38
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    Photosynthesis research 25 (1990), S. 77-82 
    ISSN: 1573-5079
    Keywords: autobiography ; chloroplast structure ; chloroplast proteins ; chloroplast lipids ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An autobiographic report of studies on the elucidation of the structure of the chloroplast is presented here. It deals with the discovery of the lamellar structure of chloroplasts by polarization-microscopy, the representation of their layer-like structure with the ultraviolet microscope and the results obtained by the electron-microscope leading to the discovery of the structural elements of the lamellar system. These lamellar structures were in the form of vesicles, and were named thylakoids. Isolation of the chloroplasts made it possible to determine their chemical composition. Amphiphilic lipids, together with water, create bimolecular layers and, therefore, are responsible for the structure of the thylakoid-membranes. The role of membrane proteins was emphasized. The isolation of the individual polypeptides was possible after dissociation in sodium-dodecyl-sulfate. Antisera to these polypeptides were used to localize them in the membrane. These antisera are able to inhibit special steps in the electron transport. Our results of the spectroscopic examination of photosynthetic membrane components are also discussed.
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  • 39
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    Photosynthesis research 24 (1990), S. 109-113 
    ISSN: 1573-5079
    Keywords: atrazine ; diuron ; photosystem II ; photosynthesis ; thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The binding of the herbicide atrazine to thylakoid membranes is often used to quantify Photosystem II reaction centres. Two atrazine binding sites, with high and low affinities, have been observed on the D1 and D2 polypeptides of Photosystem II, respectively (McCarthy S., Jursinic P. and Stemler A. (1988) Plant Physiol. 86S:46). We have observed that the accessibility of the low-affinity binding sites is variable, being limited in freshly isolated thylakoids or in fresh frozen-thawed thylakoids, but increasing during storage of the membranes on ice. In contrast, the accessibility of the high-affinity binding sites, which are titratable at low concentrations (〈 500 nM) of herbicide, is much less variable, although the dissociation constant is greatly influenced by ethanol. We conclude that to quantify Photosystem II reaction centres by atrazine binding, it is sufficient and more reliable to assay only the high-affinity binding sites.
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  • 40
    ISSN: 1573-5079
    Keywords: cyanobacteria ; photosynthesis ; psbC gene ; psbD gene ; translational start codon ; overlapping genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The unicellular photoheterotrophic cyanobacterium Synechococcus sp. PCC 7002 was shown to encode two genes for the Photosystem II reaction center core protein D2 and one gene for the reaction center chlorophyhll-binding protein CP43. These three genes were cloned and their DNA sequences determined along with their flanking DNA sequences. Northern hybridization experiments show that both genes which encode D2, psbD1 and psbD2, are expressed at roughly equivalent levels. For each of the two psbD genes, there are 18 nucleotide differences among the 1059 nucleotides which are translated. The DNA sequences surrounding the coding sequences are nearly 70% divergent. Despite the DNA sequence differences in the genes, the proteins encoded by the two genes are predicted to be identical. The proteins encoded by psbD1 and psbD2 are ∼92% homologous to other sequenced cyanobacterial psbD genes and ∼86% homologous to sequenced chloroplast-encoded psbD genes. The single gene for CP43, psbC, overlaps the 3′ end of psbD1 and is co-transcribed with it. Results from previous sequencing of psbC genes encoded by chloroplasts suggest that the 5′ end of the psbC gene overlaps the 3′ end of the coding sequence of psbD by ∼50 nucleotides. In Synechococcus sp. PCC 7002, the methionine codon previously proposed to be the start codon for psbC is replaced by an ACG (threonine) codon. We propose an alternative start for the psbC gene at a GTG codon 36 nucleotides downstream from the threonine codon. This GTG codon is preceded by a consensus E. coli-like ribosome binding sequence. Both the GTG start codon and its preceding ribosome binding sequence are conserved in all psbC genes sequenced from cyanobacteria and chloroplasts. This suggests that all psbC genes start at this alternative GTG codon. Based on this alternative start codon, the gene product is ∼85% identical to other cyanobacterial psbC gene products and ∼77% identical to eucaryotic chloroplast-encoded psbC gene products.
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  • 41
    ISSN: 1573-5079
    Keywords: inorganic carbon transport ; light ; Chlamydomonas ; CO2 exchange ; photorespiration ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract The effect of photon flux density on inorganic carbon accumulation and photosynthetic CO2 assimilation was determined by CO2 exchange studies at three, limiting CO2 concentrations with a ca-1 mutant of Chlamydomonas reinhardiii. This mutant accumulates a large internal inorganic carbon pool in the light which apparently is unavailable for photosynthetic assimilation. Although steady-state photosynthetic CO2 assimilation did not respond to the varying photon flux densities because of CO2 limitation, components of inorganic-carbon accumulation were not clearly light saturated even at 1100 μmol photons m-2 s-1, indicating a substantial energy requirement for inorganic carbon transport and accumulation. Steady-state photosynthetic CO2 assimilation responded to external CO2 concentrations but not to changing internal inorganic carbon concentrations, confirming that diffusion of CO2 into the cells supplies most of the CO2 for photosynthetic assimilation and that the internal inorganic carbon pool is essentially unavailable for photosynthetic assimilation. The estimated concentration of the internal inorganic carbon pool was found to be relatively insensitive to the external CO2 concentration over the small range tested, as would be expected if the concentration of this pool is limited by the internal to external inorganic carbon gradient. An attempt to use this CO2 exchange method to determine whether inorganic carbon accumulation and photosynthetic CO2 assimilation compete for energy at low photon flux densities proved inconclusive.
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    Photosynthesis research 25 (1990), S. 17-24 
    ISSN: 1573-5079
    Keywords: delayed light ; oxygen evolution ; photosynthesis ; thermoluminescence (money plant, pothos aurea leaf)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of ultraviolet light on thermoluminescence, oxygen evolution and the slow component of delayed light has been investigated in chloroplasts and Pothos leaves. All peaks including peak V (48°C) were inhibited by UV. However, the peak at 48°C which was induced by DCMU was enhanced following UV irradiation of chloroplasts at ambient temperature (23°C) whereas peak II (-12°C) and peak III (10°C) which were also induced by DCMU were inhibited. Chloroplasts treated with DCMU and dark incubated for several minutes at ambient temperature prior to recording of glow curves have also shown enhancement of peak at 48°C. A slow component of delayed light and photosystem II activity of chloroplasts were inhibited by UV whereas photosystem I activity was marginally affected. These results corroborate involvement of photosystem II in generating thermoluminescence and slow components of delayed light in photosynthetic materials.
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  • 43
    ISSN: 1573-5079
    Keywords: carbon dioxide ; Glycine max ; photosynthesis ; stomatal conductances ; water potential ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO2 concentrations and CO2-assimilation rates for the two soil moisture treatments were compared during the pod elongation and seed enlargement stages of crop development. Leaf CO2-assimilation rates were measured with either ambient (340 μl CO2 l−1) or CO2-enriched (1800 μl CO2 l−1) air. Although seed yield and leaf area per plant were decreased an average of 48 and 31%, respectively, as a result of drought stress, leaf water potentials were reduced only an average of 0.27 MPa during the sampling period. Afternoon leaf CO2-assimilation rates measured with ambient air were decreased an average of 56 and 49% by soil moisture deficit for Williams 82 and A3127, respectively. The reductions in leaf photosynthesis of both cultivars were associated with similar decreases in leaf stomatal conductance and with small increases in leaf intercellular CO2 concentration. When the CO2-enriched air was used, similar afternoon leaf CO2-assimilation rates were found between the soil moisture treatments at each stage of crop development. These results suggest that photosynthetic capacity of soybean leaves is not reduced by severe soil moisture deficit when a stress develops gradually under field conditions.
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  • 44
    ISSN: 1573-5079
    Keywords: chloroplast coupling factor ; induction ; thioredoxin ; regulation ; ATP synthase ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simultaneous, non-invasive measurements were made of the rate of photosynthetic CO2 fixation and the state of activation of the chloroplast CF1CF0-ATP synthase (CF) in field-grown sunflower (Helianthus annuus L.) during the dark-to-light transition at sunrise. CO2 fixation showed a linear response with light intensity from zero to about 500–700 μE m-2 s-1. However, at light intensities of only 5–22 μE m-2 s-1, the energetic threshold for activation of the CF was found to be significantly lowered (as compared to the pre-dawn state), presumably through reduction of the regulatory sulfhdryl groups of the γ-subunit of the CF. When these studies were extended to chamber-grown plants, it was found that as little as 5 seconds of illumination at 4 μE m-2 s-1 caused apparently full CF reduction. It is clear, therefore, that the catalytic activation of CF is not rate limiting to the induction of carbon assimilation under field conditions during a natural dark-to-light transition at sunrise. A model, based on the redox properties of the regulatory sulfhydryls, was developed to examine the significance of sulfhydryl midpoint potential in explaining the differences in light sensitivity and oxidation and reduction kinetics, between the CF and other thioredoxin-modulated chloroplast enzymes. Computer simulations of the light-induced regulation of three representative thioredoxin-modulated enzymes are presented.
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  • 45
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    Plant and soil 123 (1990), S. 217-222 
    ISSN: 1573-5036
    Keywords: growth chamber ; nutrient solution ; 15NO3 assimilation ; photosynthesis ; relative growth rate ; seedlings ; short-term NaCl stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Short-term studies for comparing some primary metabolic and growth-responses to salt stress in seedlings of two maize genotypes differing in drought resistance were carried out under controlled conditions. Both genotypes revealed high yielding ability in favourable environments. Treatments: Control (Hoagland-Arnon No 1 solution) and salt stress (Hoagland-Arnon solution plus NaCl, Ψs = −0.84MPa). It was found that in both genotypes the activity of the principal metabolic pathway supplying reduced nitrogen (15N) for the synthesis of amino acids and proteins as well as the assimulatory number (14CO2—assimilation relation rate per chlorophyll unit) were decreased under the effect of the stress. These effects were more marked in the resistant genotype. In this genotype the stress induced metabolic activity decline was accompanied by a corresonding reduction of the relative growth rate. Conversely, continuing growth, resulting probably from accumulation of solutes, was observed in the susceptible genotype. On the basis of these and other observations it is assumed that the resistant genotype manifests short-term energy saving stress reactions.
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  • 46
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    Plant and soil 128 (1990), S. 283-286 
    ISSN: 1573-5036
    Keywords: effectiveness ; isoenzymes ; Pisum sativum ; restriction fragment length polymorphism ; RFLP ; Rhizobium leguminosarum bv. viceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Twenty isolates of Rhizobium leguminosarum bv. viceae were isolated at random from one field and examined for symbiotic plasmid fragment length polymorphisms and for isoenzyme patterns. The latter are most probably chromosome markers. With one exception both methods separated the isolates into the same 13 different groups. The largest group was represented 7 times according to isoenzymes and 8 times according to RFLP. This fixed non-random association of plasmid and chromosomal genotypes is consistent with a clonal population structure; it indicates limited exchange of plasmids under natural conditions. Seventeen isolates of 11 groups were highly effective and 2 isolates in one group almost ineffective.
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  • 47
    ISSN: 1573-5036
    Keywords: calcium ; copper ; desert ; ground water ; iron ; magnesium ; manganese ; nitrogen ; phosphorus ; Prosopis glandulosa ; rooting patterns ; sodium ; symbiotic nitrogen fixation ; trace metals ; water table ; water use efficiency ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mesquite plants (Prosopis glandulosa var. Torreyana) were grown in 2-m long columns 20 cm in diameter, and provided with a constant, stable ground water source 10 cm above the sealed base of the column. Ground water contained 0, 1 or 5 mM nitrate, or a mixed salt solution (1.4, 2.8, or 5.6 dS m-1) with the ionic ratios of ground water found in a field stand of Prosopis at Harper's Well (2.8 dS m-1). Water uptake in the highly salinized columns began to decrease relative to low salt columns when soil salinity probes 30 cm above the column base read approximately 28 dS m-1, a potential threshold for mesquite salt tolerance. Prosopis growth increased with increasing nitrate, and decreased with increasing salinity. Water use efficiency was little affected by treatment, averaging approximately 2 g dry matter L-1 water used. Most fine roots were recovered from a zone about 25 cm above the ground water surface where water content and aeration appeared to be optimal for root growth. Root-shoot ratio was little affected by nitrate, but increased slightly with increasing salinity. Plant tissue P concentrations tended to increase with increasing salinity and decrease with increasing N, approaching potentially deficient foliage concentrations at 5 mM nitrate. The whole-plant leaf samples increased in sodium concentration both with added salt and with added nitrate. Foliar manganese concentrations increased with increasing salt in the absence of nitrate. Concentrations of sodium in leaves were low (〈10 g kg-1), considering the high salt concentrations in the ground water. Prosopis appears to exclude sodium very effectively, especially from its younger leaves. Although Prosopis is highly salt tolerant, the degree to which it utilizes soil nitrate in place of biologically fixed N may lower its salinity tolerance and affect its nutrient relations in phreatic environments.
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  • 48
    ISSN: 1573-5036
    Keywords: grassland ; leaching ; leaf litter ; macro-organic matter ; mineralization ; nitrogen ; ploughing ; roots ; stubble
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The amounts of organic matter in the stubble, litter, root and soil macro-organic matter fractions of two swards of perennial ryegrass that had received normal applications of either fertilizer or cattle urine were, on average for the four fractions, about 3000, 500, 11,500 and 8,800 kg ha−1. The swards had been established 8 or 15 years previously and each was sampled at intervals over a period of about one year. The amounts of N contained in the four fractions were, on average, 68, 12, 249 and 240 kg ha−1, a total of 569 kg N ha−1. With other swards, increasing rates of application of fertilizer N were found to have little effect on the amounts of organic matter in stubble and roots. Concentrations of N in the organic matter of the stubble and roots, however, increased significantly with increasing rate of fertilizer application, though, with stubble, moderate rates of application had little effect. Assessments based on these data, together with other published information, indicate that the amount of N mineralized from the combined stubble, litter, root and macro-organic matter fractions during the first year after ploughing may range from about 40 kg to at least 360 kg N ha−1 depending on the age of the sward and its recent management. The amount mineralized is likely to increase with age of sward, with increasing rate of fertilizer N and with utilisation by grazing rather than cutting.
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  • 49
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    Plant and soil 123 (1990), S. 155-159 
    ISSN: 1573-5036
    Keywords: cultivars ; nitrogen ; roots ; tropic climate ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Due to the high price of fertilizer the input of N for grain maize production must be kept low in many parts of the world. Low input cultivars have been suggested to meet this requirement. Screening of a group of tropical cultivars revealed two high input, two low input and two intermediate cultivars with regard to N utilization. One of the causes of an interaction between genotype and N fertilization might be differences in root morphology. Screening for such differences at an early seedling stage would facilitate the selection for low input varieties. This hypothesis was tested by growing seedlings of the six varieties at different levels of N until the fourth leaf stage. There was no significant interaction between genotypes and N supply. At low and medium N supply, the total seedling biomass was the same but at low N a higher proportion of dry weight was found in the roots. Total biomass was reduced at high N. Low input and intermediate cultivars had higher shoot and root dry weights than did high input cultivars but no significant differences in root surface area were found. Root surface area was greatest at low N. Number and total length of seminal roots were significantly lower for high input varieties which, in combination with a relatively high root surface area, points to an intensive root type.
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  • 50
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    Plant and soil 124 (1990), S. 33-37 
    ISSN: 1573-5036
    Keywords: leaf area ; nitrogen ; mineral nutrition ; phosphorus ; photoperiod ; Triticum ; wheat ; spikelet initiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of N and P on the number of spikelets of spring wheat (Triticum aestivum L.), grown in nutrient solution, were studied under 8 h and 16 h photoperiods. The effect of P was apparent only at a high rate of N supply and the effects of N were increased significantly at a high rate of P supply. Increasing N supply increased the number of spikelets due to a promotion of the rate of spikelet initiation. It also increased the leaf-blade area and the dry matter weight of the plants at the stage of terminal spikelet initiation. These effects of N were much greater under the short photoperiod than under the long photoperiod. The practical significance of these findings for winter-grown wheat in temperate regions is pointed out.
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  • 51
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    Plant and soil 128 (1990), S. 97-101 
    ISSN: 1573-5036
    Keywords: calcium ; magnesium ; nitrogen ; nitrogen saturation ; Norway spruce ; nutritional imbalance ; Picea abies ; soil solution ; sulphur/nitrogen ratio ; tree nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Differences in nitrogen cycling and in the nutrition of trees are significantly coupled to the levels of nitrogen input and to the nitrate levels in the soil solution. Relatively high nitrogen supply can cause unbalanced nutrition on sites which contain either low or moderate amounts of other nutrients. This is indicated by low cation/nitrogen ratios in foliage and also by the S/N ratio falling temporarily below 0.030.
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  • 52
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    Euphytica 51 (1990), S. 33-39 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; photosynthesis ; chlorophyll fluorescence ; harvest index ; biological yield ; economic yield ; short straw ; dwarfism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Improvements in bread wheat productivity have been related to changes in plant morphology and function associated with a large increase in the harvest index for a more or less constant biological yield. The appearance of short genotypes possessing dwarfism genes may modify markedly the objectives of breeding as the upper limits of the harvest index are approached. The aim of the investigations presented here was to identify some contrasts between short and tall genotypes in terms of the physiological characteristics associated with grain yield, so as to orientate more efficiently the selection of genotypes, with or without dwarfism genes, for productivity. Various parameters of flag leaf functioning (photosynthesis rate, chlorophyll fluorescence index, leaf area duration) were related to the biological and economic yields and the harvest index for two groups of genotypes that were differentiated by their height. For all genotypes, the relationships between the various traits and the grain yield were difficult to ascertain. For the tall genotypes without dwarfism genes, the classical relationships between grain yield, harvest index, flag leaf area duration and net photosynthesis rate were confirmed. Moreover, the rate of chlorophyll fluorescence decrease (Rfd) during the slow Kautsky kinetics phase, which is representative of the leaf photosynthesis at low light, was found to be an excellent marker of economic yield. Chlorophyll fluorescence decrease was closely related to grain yield and also with other factors that are known to be important in its expression (harvest index, flag leaf area duration). In very short genotypes, the biological yield and directly related factors (leaf area, plant height) were the main parameters associated with economic yield, since the harvest index had approached its upper limit. The selection of short genotypes must therefore maintain the biological yield through an increase in the size of the aerial organs to counterbalance the decrease in height.
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  • 53
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    Photosynthesis research 23 (1990), S. 131-162 
    ISSN: 1573-5079
    Keywords: photosynthesis ; Photosystem II ; review ; structure/function aspects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the last few years our knowledge of the structure and function of Photosystem II in oxygen-evolving organisms has increased significantly. The biochemical isolation and characterization of essential protein components and the comparative analysis from purple photosynthetic bacteria (Deisenhofer, Epp, Miki, Huber and Michel (1984) J Mol Biol 180: 385–398) have led to a more concise picture of Photosystem II organization. Thus, it is now generally accepted that the so-called D1 and D2 intrinsic proteins bind the primary reactants and the reducing-side components. Simultaneously, the nature and reaction kinetics of the major electron transfer components have been further clarified. For example, the radicals giving rise to the different forms of EPR Signal II have recently been assigned to oxidized tyrosine residues on the D1 and D2 proteins, while the so-called Q400 component has been assigned to the ferric form of the acceptor-side iron. The primary charge-separation has been meaured to take place in about 3 ps. However, despite all recent major efforts, the location of the manganese ions and the water-oxidation mechanism still remain largely unknown. Other topics which lately have received much attention include the organization of Photosystem II in the thylakoid membrane and the role of lipids and ionic cofactors like bicarbonate, calcium and chloride. This article attempts to give an overall update in this rapidly expanding field.
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  • 54
    ISSN: 1573-5079
    Keywords: photosynthesis ; thermal emission ; P700 ; quantum yield ; energy conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thermal emission and photochemical energy storage were examined in photosystem I reaction center/core antenna complexes (about 40 Chl a/P700) using photoacoustic spectroscopy. Satisfactory signals could only be obtained from samples bound to hydroxyapatite and all samples had a low signal-to-noise ratio compared to either PS I or PS II in thylakoid membranes. The energy storage signal was saturated at low intensity (half saturation at 1.5 W m-2) and predicted a photochemical quantum yield of 〉90%. Exogenous donors and acceptors had no effect on the signal amplitudes indicating that energy storage is the result of charge separation between endogenous components. Fe(CN)6 -3 oxidation of P700 and dithionite-induced reduction of acceptors FA-FB inhibited energy storage. These data are compatible with the hypothesis that energy storage in PS I arises from charge separation between P700 and Fe-S centers FA-FB that is stable on the time scale of the photoacoustic modulation. High intensity background light (160 W m-2) caused an irreversible loss of energy storage and correlated with a decrease in oxidizable P700; both are probably the result of high light-induced photoinhibition. By analogy to the low fluorescence yield of PS I, the low signal-to-noise ratio in these preparations is attributed to the short lifetime of Chl singlet excited states in PS I-40 and its indirect effect on the yield of thermal emission.
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  • 55
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    Photosynthesis research 25 (1990), S. 225-232 
    ISSN: 1573-5079
    Keywords: state 1-state 2 transitions ; Mutant nicotiana (tobacco aurea mutant Su/su) ; photoacoustics ; photosynthesis ; energy distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photoacoustic detection of oxygen evolution and Emerson enhancement in state 1 and state 2 were compared in a tobacco wild type and mutant (Su/su) deficient in chlorophyll. The mutant shows smaller changes in the distribution of excitation energy between the two photosystems than the wild type. Analysis of Emerson enhancement saturation curves indicates that in the mutant which is deficient in grana partitions and shows less stacking, state 1-state 2 transitions reflect changes in the yield of energy transfer from PS II to PS I (spillover). On the other hand, the wild type containing large grana shows changes in absorption cross-sections of the two photosystems upon state transitions. NaF, a specific phosphatase inhibitor, blocks the transition to state 1, indicating that LHC II phosphorylation has a role in excitation energy regulation in both the mutant as well as the wild type. It is demonstrated that N-ethylmaleimide, a specific sulfhydryl reagent, blocks the transition to state 2, suggesting that a disulfide-sulfhydryl redox couple activates the LHC II kinase in vivo.
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  • 56
    ISSN: 1573-5079
    Keywords: LED-Array Spectrophotometer ; difference spectroscopy ; time resolved spectra ; photosynthesis ; cytochrome b/f complex ; cytochrome b 559 ; heat-stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new type of computer controlled spectrophotometer is described which is based on an array of independent, monochromatic pulsed light sources consisting of light emitting diodes (LED) equipped with narrow band interference filters. The LEDs are sequentially pulsed at a high repetition rate. The absorbance information at specific wavelengths is sampled in the μs-time range, using a computer-controlled, highly selective technique of synchronous amplification. A first prototype of this LED Array Spectrophotometer allows simultaneous recording of kinetic changes at 16 different wavelengths in the range from 530 to 600 nm, with a time resolution of 1 ms/point. Special features of the new type of spectrophotometer are: Weak integrated measuring light intensity, high signal/noise ratio even with scattering samples like intact leaves, active baseline adjustment by LED current regulation, computer control of system operation and data analysis. To deconvolute the complex absorbance changes in the cytochrome α-band region, ‘standard spectra’ of the major components are stored in computer memory and used for curve fitting of difference spectra and kinetic changes. As an example of application, the light-induced absorbance changes in a heat-pretreated spinach leaf are analysed. The system effectively separates specific absorbance changes of C550, cyt f, cyt b 559 and cyt b 563 from a large background of non-specific changes.
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  • 57
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    Photosynthesis research 26 (1990), S. 59-66 
    ISSN: 1573-5079
    Keywords: Video imaging ; fluorescence induction kinetics ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A fluorescence video imaging system utilizing relatively inexpensive commercial components is described. The instrument utilizes a black and white CCD video camera detector, a commercial video imaging board and a IBM-AT compatible computer. The color output of the imaging board greatly aids in the users ability to visually discriminate areas of interest in the video field. Software development that enables the user to capture kinetic traces in real time from the video images is also described. The system is used to monitor fluorescence from photosynthetic systems. The usefulness of the system in screening for photosynthetic mutants is also demonstrated. The cost of the system can be kept below $12,000.
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  • 58
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    Photosynthesis research 25 (1990), S. 119-128 
    ISSN: 1573-5079
    Keywords: adaptation ; genetic variation ; photosynthesis ; wild emmer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wild emmer wheat (Triticum dicoccoides Korn) has shown wide genetic diversity for disease resistance and morpho-physiological traits of economic importance. Our objectives were to test for genetic variation (VG) in photosynthetic characteristics residing within and between native populations sampled from three ecogeographical regions of Israel, and to identify potential sources of high photosynthetic efficiency for future wheat improvement. Accessions sampled in the center of wild emmer distribution (upper Jordan Valley) in a relatively narrow geographical range showed the greatest diversity in CO2-assimilation rate per unit leaf area (A) or per unit chlorophyll (A/Chl). Genetic variation was absent for internal CO2 concentration (Ci) and water-use efficiency (WUE) and generally lacking for stomatal conductance (gs). Leaf area, although quite variable, was not a significant cofactor in assessing genetic potential for photosynthesis. Accessions within a given population showed 10-times more variation in A and A/Chl than populations sampled from different locations in a region. Accessions with the highest photosynthetic efficiency were derived from upland steppic populations located in marginal habitats extending southward into Israel. Some accessions having high photosynthetic capacity (A=32 μmol m-2 s-1) with no significant reduction in leaf size constitute a potentially valuable genetic resource yet untapped for genetic improvement of hexaploid (T. aestivum L.) wheat.
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  • 59
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    Photosynthesis research 25 (1990), S. 309-316 
    ISSN: 1573-5079
    Keywords: photoacoustics ; photosynthesis ; O2-evolution ; gas-exchange ; heat evolution ; chlorophyll fluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A newly developed photoacoustic system for measurement of photosynthetic reactions in intact leaves is described. The system is based on pulsed light-emitting diodes, the pulse program and pulse response analysis being computer controlled. Separation of various components in the overall photoacoustic signal is achieved by curve fitting analysis of the responses following individual measuring light pulses in the millisecond time domain. This procedure is in distinction to the conventionally used analysis in the frequency domain, with the advantage that various signal components are obtained by on-line deconvolution, yielding simultaneous recordings of photothermal (complement of energy storage) and photobaric (evolution and uptake) signals. The basic components of the new system are described by block diagrams and the principal steps for deconvolution of the overall photoacoustic response are outlined. An example of application with simultaneous recording of chlorophyll fluorescence is given. It is apparent that the photobaric uptake component represents a significant part of the overall signal, particularly during induction of photosynthesis after dark-adaptation. This component probably contains not only O2-uptake but uptake of CO2 as well.
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  • 60
    ISSN: 1573-5079
    Keywords: Chloroplasts ; Kranz anatomy ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several leaf anatomical and ultrastructural characteristics usually related with photosynthetic capacity were examined in two Flaveria species with strong differences in anatomy and their F1 hybrid. Flaveria trinervia (Spreng.) Mohr (C4) was the female parent and F. brownii A.M. Powell (C4-like) was the male parent. Quantitative anatomical analysis was made on transverse sections of leaves at both the light and electron microscope level. Four kinds of photosynthetic tissues were considered: bundle sheath (BS), mesophyll adjacent to the BS, mesophyll not adjacent to the BS, and larger spongy mesophyll cells. Flaveria trinvervia partitioned a larger proportion of its photosynthetic cells to BS and the mesophyll layer adjacent to BS and also possessed larger chloroplasts, especially in BS, than did F. brownii. These results suggest that although F. brownii is very C4-like, its anatomy is not as completely C4 as is the case for F. trinervia. In the F1 hybrid the relative contribution of the different tissues to the total photosynthetic tissue volume and area per unit leaf area was quite similar to that of F. trinervia. On the other hand, the chloroplast density and size of the F1 hybrid were fairly similar to those of F. brownii, especially in BS. Thus, there was no evidence of maternal inheritance in the chloroplast characteristics studied. A negative correlation (P〈0.05) between chloroplast size and density was observed among species and relicates within each kind of tissue. This correlation was highest (r=−0.94, P〈0.001) for the BS and when values were plotted on a logarithmic scale. Thus, higher chloroplast numbers for F. brownii and the F1 hybrid were offset by larger chloroplasts in F. trinervia. Less complete C4 photosynthesis in F. brownii may be partially due to incomplete development of Kranz anatomy usually associated with C4 photosynthesis.
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  • 61
    ISSN: 1573-5117
    Keywords: phytoplankton collapses ; hypertrophic ; nitrogen ; phosphorus ; sedimentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Short-term changes in phytoplankton and zooplankton biomass have occurred 1–3 times every summer for the past 5 years in the shallow and hypertrophic Lake Søbygård, Denmark. These changes markedly affected lake water characteristics as well as the sediment/water interaction. Thus during a collapse of the phytoplankton biomass in 1985, lasting for about 2 weeks, the lake water became almost anoxic, followed by rapid increase in nitrogen and phosphorus at rates of 100–400 mg N M−2 day−1 and 100–200 mg P m−1 day−1. Average external loading during this period was about 350 mg N m−2 day−1 and 5 mg P m−2 day−1, respectively. Due to high phytoplankton biomass and subsequently a high sedimentation and recycling of nutrients, gross release rates of phosphorus and nitrogen were several times higher than net release rates. The net summer sediment release of phosphorus was usually about 40 mg P m−2 day−1, corresponding to a 2–3 fold increase in the net phosphorus release during the collapse. The nitrogen and phosphorus increase during the collapse is considered to be due primarily to a decreased sedimentation because of low algal biomass. The nutrient interactions between sediment and lake water during phytoplankton collapse, therefore, were changed from being dominated by both a large input and a large sedimentation of nutrients to a dominance of only a large input. Nitrogen was derived from both the inlet and sediment, whereas phosphorus was preferentially derived from the sediment. Different temperature levels may be a main reason for the different release rates from year to year.
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  • 62
    ISSN: 1573-5117
    Keywords: submerged macrophyte ; Ceratophyllum demersum ; litter ; decomposition ; pyrolysis mass spectra ; residual mass ; carbon ; nitrogen ; phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study was made of decomposition ofCeratophyllum demersum litter over a 17-day period under controlled conditions of temperature and oxygen (5, 10 and 18 °C; aerobic and anaerobic) and over a 169-day period in the field (Lake Vechten, The Netherlands). Litter, water and sediment were sampled on the 0, 2, 4, 7 and 17th day under controlled conditions and on the 0, 17, 49, 127 and 169th day in the field. The litter was analyzed quantitatively for dry mass, ash, carbon, nitrogen, phosphorus and qualitatively of organic composition by pyrolysis mass spectrometry. The water was analyzed for the elemental concentrations of organic carbon (total and dissolved), nitrogen (total, ammonia and particulate) and phosphorus (total and orthophosphate) and for the concentrations of photosynthetic pigments and bacteria. The sediment was analyzed for the elemental concentrations of nitrogen, carbon and phosphorus, and for bacterial numbers. The pattern of litter mass loss fitted an exponential model fairly well. Mass decreased faster under controlled aerobic than under anaerobic conditions and the decrease was stimulated by increasing temperature, relatively more in the range of 5 to 10 °C (by 20%) than in the range of 10 of 18 °C (by 2%). The residual mass ranged from 73 to 43% of initial under controlled aerobic conditions and from 84 to 65% under anaerobic conditions after 17 days. It decreased far less in the field, to 38% of initial mass in the field after 169 days. The litter initially lost a carbohydrate fraction by leaching in all treatments. The protein content decreased initially as well but increased subsequently at increasing temperature stimulated under anaerobic conditions. The changes in organic composition were correlated with those in nitrogen but not with those in carbon and phosphorus contents. The organic composition of litter incubated in the field differed from that of litter incubated in the laboratory. The field residues contained less proteinaceous material than the laboratory residues. The changes in carbon, nitrogen and phosphorus concentrations in the litter showed different patterns. The carbon concentration generally increased, the nitrogen concentration initially dropped and increased subsequently, and the phosphorus concentration initially dropped and remained relatively constant subsequently. Chemical immobilization of the decomposition process may have occurred in the laboratory, but was unlikely in the field. Carbon, nitrogen and phosphorus left the litter initially largely in particulate form and were recovered in the water. The ratio dissolved: total nutrient concentration was lower under controlled aerobic than under anaerobic conditions. Increasing temperature stimulated bacterial use of dissolved organic carbon and nitrogen. A rapid nutrient flow occurred from macrophyte litter, via water to sediment. The phytoplankton biomass in the water was greatly stimulated by substances freed from the decomposing litter. Diatoms increased generally relatively more than green algae, predominating alternatively with green algae under aerobic conditions and continuously under anaerobic conditions. Bacterial numbers in the water initially increased, partly due to transgression of bacteria from the sediment-water interface to the water and partly due to an actual increase in community biomass. The bacteria returned largely to the sediment-water interface, stimulated by increasing temperature, as most of the substrate readily usable by them had left the litter in the litter-bag and was associated with the upper sediment layers. It is feasible that the annual die-off of theC. demersum population of Lake Vechten barely affects nutrient cycling in the lake, because the contribution to the nutrient pools of the lake when fully mixed is only small. However, small particles originating from decomposingC. demersum litter may influence the lake considerably by decreasing water transparency and serving as a food source for filter-feeders and detritivorous macrofauna.
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  • 63
    ISSN: 1573-5117
    Keywords: hydrography ; nutrients ; photosynthesis ; subsurface chlorophyll maxima ; southeastern Brazil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The physical and chemical environment, and the phytoplankton primary production of southeastern Brazil were studied in relation to the general oceanographic structure during two research cruises (winter and summer). In each cruise, a total of 91 stations were occupied. Data were collected on the spatial distribution of nutrients, phytoplankton biomass and photosynthetic capacity over the coastal, shelf and oceanic areas off São Paulo, Paraná and Santa Catarina States. During wintertime, the mixing processes between tropical warm waters of the Brazil Current and subantarctic waters of the Malvinas Current formed strong environmental gradients. The drainings of Rio de La Plata and Lagoa dos Patos are transported northwards by coastal currents, enriching the shelf waters off Santa Catarina State with inorganic nutrients and consequently increasing the chlorophyll ‘a’ to the highest concentrations (〉 3.5 mg m −3) measured during the two cruises. In slope waters chlorophyll values were always low (0.05–0.45 mg m −3). The chlorophyll within the euphotic layer varied from 8.8–36.7 and 1.2–18.5 mg m−2 during winter and summer, respectively. The surface photosynthetic rates during winter and summer cruises ranged respectively from 0.21–9.17 and 0.66–19.60 mgC/mgChl.a/h. The mean rates were higher in nearshore waters and decreased seaward. The thermal structure of the water column affected the vertical distribution of chlorophyll a and photosynthesis within the euphotic zone; During unstratified periods (winter) they were uniformly distributed but the occurrence of subsurface peaks of chlorophyll and strong photosynthetic inhibition of low light adapted cells in deeper layers are associated to the seasonal thermocline. Occasionally, upwelling of deep waters from shelf break enriched the deeper euphotic layers in offshore areas. Intensive upwelling was observed off Paranagua Bay (Parana State) and the mechanisms of its formation are discussed.
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  • 64
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    Hydrobiologia 202 (1990), S. 61-69 
    ISSN: 1573-5117
    Keywords: Gulls ; phosphorus ; nitrogen ; eutrophication ; excretion ; nutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nutrient excretion rates and the annual contribution of P from the feces of the gullsLarus argentatus andL. marinus (and of N fromL. argentatus) to the nutrient budget of Gull Pond (Wellfleet), a soft water seepage lake, have been estimated. Intensive year-round gull counts by species were combined with determinations of defecation rate and the nutrient content of feces to quantitatively assess the P loading rates associated with regular gull use of this coastal pond on a seasonal and annual basis. Total P loading from gulls was estimated to be 52 kg yr−1, with 17 kg fromL. argentatus and 35 kg fromL. marinus, resulting from about 5.0 × 106 h yr−1 and 1.7 × 106 h yr−1 of pond use. This compares with P loading estimates of 67 kg yr−1 from upgradient septic systems, 2 kg yr−1 from precipitation and 3 kg yr−1 from unpolluted ground water. Fifty-six percent of annual gull P loading was associated with migratory activity in late fall. Estimated annual N loading byL. argentatus was 14 kg TKN, 206 g NO3-N, and 1.85 g g NH3-N.
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  • 65
    ISSN: 1573-5117
    Keywords: Ecklonia cava ; Phaeophyta ; photosynthesis ; respiration ; seaweed ; zoosporangial sori
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthetic rates were compared between Ecklonia cava bladelets with and without zoosporangial sori sampled from the subtidal zone (about 5 m deep) in Nabeta Bay, Shimoda, Japan. Photosynthetic rates of bladelets were lower in the sorus portion than in the non-sorus portion on the basis of area, dry weight and chlorophyll a. Respiration rates were higher in the sorus portion than in the non-sorus portion on the basis of area and chlorophyll a, whereas they were almost the same on a dry weight basis. The differences were mainly due to a large difference in dry weight per unit bladelet area between the sorus and the non-sorus portion. Light compensation points were higher in the sorus portion than in the non-sorus portion.
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  • 66
    ISSN: 1573-5117
    Keywords: nitrogen ; phosphorus ; bluegill ; plankton ; mesocosm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We conducted an outdoor mesocosm experiment of factorial design consisting of three levels of nutrient supply (no nutrient addition and additions of nitrogen and phosphorus in ratios of 10:1 and 45:1) cross-classified with two levels of bluegill (Lepomis macrochirus) (presence and absence). Nutrient supply significantly affected total phosphorus (TP), total nitrogen (TN), TN: TP ratio, turbidity, Secchi depth, phytoplankton chlorophyll, filamentous blue-green algae, periphyton chlorophyll, Asplanchna and non-predatory rotifers. The presence of bluegill significantly increased TP, turbidity, diatoms, unicellular green algae, colonial blue-green algae, filamentous blue-green algae, periphyton chlorophyll, Asplanchna and non-predatory rotifers, and decreased Secchi depth, cladocerans, cyclopoid copepodids, copepod nauplii and chironomid tube densities. Nutrient supply and fish effects were not independent of each other as shown by significant nutrient × fish interaction effects for TP, Secchi depth, filamentous blue-green algae, periphyton chlorophyll, Asplanchna and non-predatory rotifers.
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  • 67
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    Hydrobiologia 202 (1990), S. 61-69 
    ISSN: 1573-5117
    Keywords: Gulls ; phosphorus ; nitrogen ; eutrophication ; excretion ; nutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nutrient excretion rates and the annual contribution of P from the feces of the gulls Larus argentatus and L. marinus (and of N from L. argentatus) to the nutrient budget of Gull Pond (Wellfleet), a soft water seepage lake, have been estimated. Intensive year-round gull counts by species were combined with determinations of defecation rate and the nutrient content of feces to quantitatively assess the P loading rates associated with regular gull use of this coastal pond on a seasonal and annual basis. Total P loading from gulls was estimated to be 52 kg yr−1, with 17 kg from L. argentatus and 35 kg from L. marinus, resulting from about 5.0 × 106 h yr−1 and 1.7 × 106 h yr−1 of pond use. This compares with P loading estimates of 67 kg yr−1 from upgradient septic systems, 2 kg yr−1 from precipitation and 3 kg yr−1 from unpolluted ground water. Fifty-six percent of annual gull P loading was associated with migratory activity in late fall. Estimated annual N loading by L. argentatus was 14 kg TKN, 206 g NO3-N, and 1.85 g g NH3-N.
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  • 68
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    Hydrobiologia 203 (1990), S. 93-97 
    ISSN: 1573-5117
    Keywords: sediments ; nitrogen ; phosphorus ; nutrient limitation ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A diffusion enrichment technique is presented which allows for chemical enrichment of soft surficial and shallow subsurface sediments and subsequent measurement of O2 production. The sediment is enriched by inserting a perforated tube containing dialysis tubing filled with a nutrient/agar mixture. O2 production by surficial sediment is measured using an inverted, translucent, polyethylene chamber over the sediment. The inside of the chamber contains a collapsible bag connected to the water outside the chamber. When water overlying the sediment is withdrawn from a sampling port, it is displaced with water from outside the chamber, thus preventing contamination of water samples with pore water from below. The technique was tested by enriching near-shore sediments in a large oligotrophic lake with inorganic N and P. NHinf4/p+ additions significantly stimulated benthic primary production as measured by 02 production, whereas enrichment with POinf4/3- had no effect.
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  • 69
    ISSN: 1573-5117
    Keywords: Gracilaria ; growth ; light ; photosynthesis ; RuBPcase ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The local species Gracilaria conferta and the foreign G. verrucosa were grown together under a wide range of photon flux density and temperature conditions. Gracilaria verrucosa showed a higher growth rate, especially under low temperatures, and higher photosynthetic performances as well as higher ribulose-1,5-bisphosphate carboxylase activity as compared with G. conferta. Gracilaria verrucosa also showed a better quality and yield of agar, suggesting that this species could be more suitable than G. conferta for outdoor cultivation in Israel and may improve winter growth in ponds. Growth rate and agar quality (gel strength) were rated as the most suitable characteristics influencing the preference of strains for outdoor cultivation.
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  • 70
    ISSN: 1573-5117
    Keywords: nitrogen ; phosphate ; phytoplankton ; Mediterranean lagoon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A network of 63 stations was used on four occasions (June 1986, October 1986, February 1987, and May 1987) to study the spatio-temporal distribution of inorganic nutrients in Thau Lagoon (‘l'étang de Thau’), which covers 7500 hectares on the French Mediterranean coast. Three environmental factors, revealed by multiple regression models, govern the distributions observed. Allochthonous inputs from the watershed enrich the environment with nitrogen and phosphorus compounds in the winter and autumn. Internal sources are essentially localized in the shellfish breeding zone of the lagoon. In the summer, shellfish excretions and the rapid remineralization of organic deposits produce ammonium ions. Uptake by phytoplankton has a much larger impact on the seasonal variation of inorganic nitrogen than on that of phosphorus; the latter is present in excess in the lagoon waters. Thus, nitrogen appears to be the primary limiting nutrient for the development of the chorophyllous biomass.
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  • 71
    ISSN: 1573-5117
    Keywords: Gelidium ; photosynthesis ; physiological responses ; Pterocladia ; Rhodophyta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Manometric studies were conducted on Pterocladia capillacea, Gelidium latifolium and Gelidium spinulosum from the Azores, Portugal to determine optimal values of temperature, light and salinity for growth. Physiological responses were considered in relation to vertical distribution patterns of these species commonly observed throughout the Azores. Optimal parameters for the growth of Pterocladia capillacea, Gelidium latifolium and G. spinulosum were 17 to 25 °C, a photon flux density between 200 and 300 µmol m−2 s−1 and salinities of 25 to 35‰.
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  • 72
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    Biogeochemistry 10 (1990), S. 67-79 
    ISSN: 1573-515X
    Keywords: deserts ; ecosystem ; nitrogen ; nutrient cycling ; soils ; southwestern United States
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract A lower limit for nitrogen loss from desert ecosystems in the southwestern United States was estimated by comparing nitrogen inputs to the amount of nitrogen stored in desert soils and vegetation. Atmospheric input of nitrogen for the last 10 000 years was conservatively estimated to be 2.99 kg N/m2. The amount of nitrogen stored in desert soils was calculated to be 0.604 kg N/m3 using extant data from 212 profiles located in Arizona, California, Nevada, and Utah. The average amount of nitrogen stored in desert vegetation is approximately 0.036 kg N/m2. Desert conditions have existed in the southwestern United States throughout the last 10 000 years. Under such conditions, vertical leaching of nitrogen below a depth of 1 m is small (ca. 0.028 kg N/m2 over 10 000 years) and streamflow losses of nitrogen from the desert landscape are negligible. Thus, the discrepancy found between nitrogen input and storage represents the amount of nitrogen lost to the atmosphere during the last 10 000 years. Loss of nitrogen to the atmosphere was calculated to be 2.32 kg N/m2, which is 77% of the atmospheric inputs. Processes resulting in nitrogen loss to the atmosphere from desert ecosystems include wind erosion, ammonia volatilization, nitrification, and denitrification. Our analysis cannot assess the relative importance of these processes, but each is worthy of future research efforts.
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  • 73
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    Biogeochemistry 11 (1990), S. 1-22 
    ISSN: 1573-515X
    Keywords: disturbance ; ecosystems ; forests ; indirect interactions ; landscape ecology ; Minnesota ; nitrogen ; nutrient cycling ; path analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Path analysis was used to determine the importance of long-term disturbance regime and the relative importances of correlations among vegetation patterns, disturbance history, and nitrogen (N) mineralization in old-growth forests of northwestern Minnesota. Leaf biomass (estimated by allometric equations), fire history (from fire scars on Pinus resinosa trees), and N mineralization rates (estimated from incubationsin situ) were determined from sample plots dominated by Betula papyrifera, Populus tremuloides, andP. grandidentata a mixture ofAcer saccharumandTilia americana, or Quercus borealis andOstrya virginiana. Results showed that topographic and soil-moisture controls on N mineralization, vegetation patterns, and disturbance are substantially stronger than is suggested by direct correlation. Indirect interactions among ecosystem variables played in important role. These interactions probably include the tendency for species that cycle large amounts of N to colonize more mesic sites that burned rarely in the past. Soil moisture was correlated both directly with N mineralization and indirectly, through its effects on vegetation pattern, and thus, litter quality. Although disturbance regime also depended on topography, the strengths of relationships between disturbance regime and other variables were relatively weak. These dependencies suggested that long-term fire regime is probably more a consequence than a cause for vegetation and fertility patterns. Topography, through its effects on soil moisture and microclimate, is an overriding influence on ecosystem properties, which in turn influence fire regime.
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  • 74
    ISSN: 1573-515X
    Keywords: cumulative ; flow ; GIS ; landscape ; lead ; nitrogen ; phosphorus ; suspended solids ; watershed ; wetlands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract A method was developed to evaluate the cumulative effect of wetland mosaics in the landscape on stream water quality and quantity in the nine-county region surrounding Minneapolis—St. Paul, Minnesota. A Geographic Information System (GIS) was used to record and measure 33 watershed variables derived from historical aerial photos. These watershed variables were then reduced to eight principal components which explained 86% of the variance. Relationships between stream water quality variables and the three wetland-related principal components were explored through stepwise multiple regression analysis. The proximity of wetlands to the sampling station was related to principal component two, which was associated with decreased annual concentrations of inorganic suspended solids, fecal coliform, nitrates, specific conductivity, flow-weighted NH4 flow-weighted total P, and a decreased proportion of phosphorus in dissolved form(p 〈 0.05). Wetland extent was related to decreased specific conductivity, chloride, and lead concentrations. The wetland-related principal components were also associated with the seasonal export of organic matter, organic nitrogen, and orthophosphate. Relationships between water quality and wetlands components were different for time-weighted averages as compared to flow-weighted averages. This suggests that wetlands were more effective in removing suspended solids, total phosphorus, and ammonia during high flow periods but were more effective in removing nitrates during low flow periods.
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  • 75
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    Biogeochemistry 11 (1990), S. 23-43 
    ISSN: 1573-515X
    Keywords: acid precipitation ; ammonium ; mass balance ; nitrate ; nitrogen ; retention
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The relative contribution of HN03 to precipitation acidity in eastern Canada has increased in recent years leading to some concern that the relative importance of NO− 3 deposition in acidification of terrestrial and aquatic ecosystems may increase. To gauge the extent of this impact, annual mass balances for N0− 3 and NH+ 4 were calculated for several forested catchments and lakes in Ontario. Retention of NH+ 4 (R NH4) by forested catchments was consistently high compared to retention of NO3 − (R NO3) which was highly variable. Retention of inorganic nitrogen was influenced by catchment grade and areal water discharge. In lakes, the reciprocals of retention of N0− 3 and NH+ 4 were linearly related to the ratio of lake mean depth to water residence time (z/τ; equal to areal water discharge), and retention did not appear to be a function of degree of acidification of the lakes. Net N consumption-based acidification of lakes, defined as the ratio of annual NH; mass to N0− 3 mass consumption, was negatively correlated with /τ and N consumption-related acidification was most likely to occur when − was 〈 1.5 m yr−1. If retention mechanisms are unaffected by changes in deposition, changes in deposition will still result in changes in surface water concentrations although the changes will be of similar proportions. Therefore, ‘NO− 3 saturation’ should not be defined by concentrations alone, but should be defined as decreasing long-term, average NO− 3 retention in streams and lakes in response to long-term increases in NO− 3 deposition. Analysis o f survey data will be facilitated by grouping lakes and catchments according to similar characteristics.
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  • 76
    ISSN: 1573-515X
    Keywords: nitrogen ; snow ; flux
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Increased emissions of nitrogen compounds to the atmosphere by human activities have been well documented. However, in order to better quantify these anthropogenic emissions, better knowledge of natural emissions rates must be known. In addition, variation in natural emissions through time should be documented. In this note we present data collected and/or analyzed by us for NO3 − in recent snow from remote regions of the world. We also summarize existing data sets from other remote regions. This is done to establish a better understanding of NO3 − deposition rates in these regions as well as to add more information to our global understanding of NO3 − deposition.
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  • 77
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    Plant cell, tissue and organ culture 21 (1990), S. 185-189 
    ISSN: 1573-5044
    Keywords: ammonium nitrate ; Malus ; nitrogen ; potassium nitrate ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of some macronutrients, especially NH4NO3 and KNO3, on root development of microcuttings from 3 apple scion cultivars is discussed. A reduction of the level of NH4NO3 in the medium from full strength to 1/4 strength significantly increased the percentage rooting of ‘Gala’ and ‘Royal Gala’, but not ‘Jonagold’. Further reduction of NH4NO3 level from 1/4 strength to zero significantly reduced the percentage of rooting in ‘Gala’ but not ‘Royal Gala’. ‘Jonagold’ rooted best at zero concentration NH4NO3. Without NH4NO3, rooting percentages were as high as 100% for all 3 cultivars when KNO3 was provided at full strength. The results show that adventitious roots can be induced on apple scion cultivars by media manipulation.
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  • 78
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    Biogeochemistry 9 (1990), S. 117-134 
    ISSN: 1573-515X
    Keywords: nitrogen ; Mediterranean ; natural versus anthropogenic atmospheric nitrogen ; atmospheric input ; riverine input ; marine ecosystems ; primary production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Bulk inorganic nitrogen deposition was monitored over a period of 3 years at the Bavella Pass (Corsica, France). Annual fluxes range between 126 and 150μmol.m−2.d-−1, increasing slightly with annual rainfall. Natural background average concentrations of rain water and associated fluxes were estimated from a classification of rain events into ‘natural’ (Oceanic and Saharan), polluted and composite. Long range transport of incoming polluted air masses increases the atmospheric wet nitrogen input by at least a factor of 1.6 in this Mediterranean area. Extrapolation of atmospheric dissolved inorganic nitrogen input to the Western Mediterranean leads to fluxes of 80 to l00μmol.m−2.d-−1. This atmospheric input is in the same order of magnitude as the inorganic nitrogen riverine input. As a consequence, the nitrogen budget for the Mediterranean has had to be reassessed. Atmospheric wet inorganic nitrogen input is of noticeable importance to marine Mediterranean ecosystems, representing on average 10 to 25% of new production in the Western Basin, with values of up to 60% in oligotrophic zones.
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    Plant cell, tissue and organ culture 22 (1990), S. 205-211 
    ISSN: 1573-5044
    Keywords: CO2 enrichment ; Cymbidium ; in vitro ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photosynthetic characteristics of the Cymbidium plantlet in vitro cultured on Hyponex-agar medium with 2% sucrose were determined based on the measurements of CO2 concentration inside and outside of the culture vessels. The CO2 measurements were made with a gas chromatograph at a PPF (photosynthetic photon flux) of 35, 102 and 226 μmol m-2 s-1, a chamber air temperature of 15, 25 and 35°C and a CO2 concentration outside the vessel of approximately 350, 1100 and 3000 ppm. The net photosynthetic rates were determined on individual plantlets and were expressed on a dry weight basis. The steady-state CO2 concentration during the photoperiod was lower inside the vessel than outside the vessel at any PPF greater than 35 μmol m-2s-1 and at any chamber air temperature. The photosynthetic response curves relating the net photosynthetic rate, PPF, and CO2 concentration in the vessel and chamber air temperature were similar to those for Cymbidium plants grown outside and other C3 plants grown outside under shade. The results indicate that CO2 enrichment for the plantlets in vitro at a relatively high PPF would promote photosynthesis and hence the growth of chlorophyllous shoots/plantlets in vitro and that the plantlets in vitro would make photoautotrophic growth under environmental conditions favorable for photosynthesis.
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    Ecological research 5 (1990), S. 111-130 
    ISSN: 1440-1703
    Keywords: Big woody materials ; Decomposition rate ; Foothill diperocarp forest ; nitrogen ; turnover time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The dynamics of aboveground big woody organs over 10 cm diameter was studied at a mature foothill dipterocarp forest in West Sumatra. The biomass of big woody organs was estimated to be 519 m3 ha−1 or 408 metric ton ha−1 by means of a pipe model theory. The diameter distribution showed a convex curve and the mode was found at a diameter of about 20 cm. The standing mass of big dead woody litter on the forest floor was 116 m3 ha−1, which accounted for 22% by voume or 9.5% by weight of the biomass of living organs respectively. Thedbh observation with two 1-ha plots for 4 yr and 5 yr respectively revealed that the average net production rate was 9.5 ton ha−1 yr−1. The death rate (7.9 ton ha−1 yr−1) accounted for 83% of the net production rate and was nearly equivalent to the decay rate (7.5 ha−1 yr−1) of dead wood on the forest floor. The balance between the death and decay rates was confirmed for each diameter class. Average turnover periods for big woody organs and dead woody litter were estimated to be 43 and 8.1 yr, respectively. Standing masses of live anddead woody materials accumulated in the study forest were approximately equal to those obtained in a mature tropical lowland rainforest, whereas the flow rates were lower, being only 70% of the corresponding values.
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  • 81
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    Nutrient cycling in agroecosystems 22 (1990), S. 29-35 
    ISSN: 1573-0867
    Keywords: Zinc ; nitrogen ; interaction ; limed ; unlimed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A green house experiment was conducted to study the interaction effect of Zn and N in wheat (S-308). Zinc was applied as ZnSO4.7H2O at 0, 5, 10 and 20 mg per kg, and nitrogen as urea at 0, 75 and 150 mg per kg. In the absence of added N and lime 5 mg Zn per kg increased the grain, straw and root weight, but the application of either N (75 and 150 mg per kg) or lime (4000 mg CaCO3 per kg), 10 mg Zn per kg responded significantly. However, when N and lime were added together, 20 mg Zn per kg increased the grain, straw and root weight significantly. Irrespective of Zn and N, the grain, straw and root weights were higher in limed that in unlimed soils. The application of N increased the Zn concentration in wheat tops and roots in unlimed soils, and decreased it in limed soils. However, because of an increase in wheat yield, the uptake of Zn by wheat tops and roots also increased with N application both in limed and unlimed soils. The addition of Zn to 10 mg per kg, increased the N concentration in the absence of N, but in the presence of N, the addition of Zn to 20 mg per kg decreased the N concentration in wheat tops and roots. The applied Zn to 10 mg per kg in unlimed soils and to 20 mg per kg in limed soils increased the N uptake by wheat tops and roots, respectively. The Zn concentration was higher in absence of lime than in its presence while a reverse trend was true for N concentration.
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  • 82
    ISSN: 1573-0867
    Keywords: Potatoes ; nitrogen ; foliar sprays ; urea ; 15N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of the timing of N fertilizer application on the uptake and partitioning of N within the crop and the yield of tubers has been studied in two experiments. In 1985 either none, 8 or 12 g N m−2 was applied and in 1986 none, 12 or 18 g N m−2. Fertilizer N was applied either at planting, around the time of tuber initiation or half at planting and the remainder in four foliar sprays of urea during tuber bulking.15N-labelled fertilizer was applied to measure the recovery of fertilizer N in the crops. There was an apparent pre-emergence loss of nitrate from the soil when N was applied at planting in 1986, thereby reducing the efficiency of fertilizer use. Applying the N at tuber initiation delayed and reduced the accumulation of N in the canopy compared with crops receiving all their fertilizer at planting. Foliar sprays of urea slightly increased both tuber yields and tuber N contents when compared to a single application at planting. The proportion of the fertilizer N recovered in the crop was little affected by the rate of N application, but a greater proportion of foliar-applied N was recovered than N broadcast at planting, due partly to pre-emergence losses of nitrate in 1986. It is suggested that late applications of N was foliar sprays can be of benefit to crops with a long growing season and reduce environmental losses of N.
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    Nutrient cycling in agroecosystems 21 (1990), S. 171-177 
    ISSN: 1573-0867
    Keywords: Bermudagrass ; nitrogen ; model ; nutrient uptake ; forages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Equations were developed to estimate concentration of nitrogen for bermudagrass forage [Cynodon dactylon, (L.) Pers.] as related to applied nitrogen level and harvest interval. Data from six field studies were used in the analysis. Estimates of N concentration in forage obtained with these equations agreed with data from other studies with fertilizer and waste application to bermudagrass (correlation coefficient of 0.94). Concentration of nitrogen decreased linearly with harvest interval (up to 6 weeks) and increased exponentially with nitrogen level. These equations should provide relevant information for crop production and environmental quality.
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  • 84
    ISSN: 1572-9567
    Keywords: Burnett apparatus ; compressibility factor ; density ; ethane ; methane ; mixtures ; nitrogen ; refractive index ; Z-meter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The European Gas Research Group (GERG; Groupe Européen de Recherches Gazières) initiated a round-robin test of six Z-meters manufactured by Desgranges et Huot, a Burnett apparatus, and an interferometric device to back up the pVT data of the Z-meters. Two gas mixtures were measured. One mixture contained 49.7 mol% of methane and 50.3 mol% of nitrogen; the second mixture 81.3 mol% of methane, 16.4 mol% of ethane, and 2.3 mol% of propane. The test temperatures were mainly 280 and 300 K for the first mixture and 290 and 320 K for the second mixture. The maximum pressures were 8 MPa for Z-meters and 12 MPa for the Burnett apparatus and the grating interferometer. The experimental compressibility factors Z of the six Z-meters are generally in agreement within ±0.05%. The agreement with the reference data from the Burnett apparatus and the refractive index measurements is also within ± 0.05%. Only two isotherms of the binary mixtures differ by about 0.1% from the other data. Recent natural gas measurements show substantially the same results.
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  • 85
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    International journal of thermophysics 11 (1990), S. 201-211 
    ISSN: 1572-9567
    Keywords: enthalpy ; heat capacity ; high temperatures ; nitrogen ; virial coefficients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Calculations of the second virial coefficients and their derivatives for the Hulburt-Hirschfelder (HH) and other accurate interaction potentials are used to determine the thermodynamic properties of nitrogen at high temperatures. Unlike the usual methods employing partition functions, which are most accurate at low temperatures where the energy levels are precisely known, the virial coefficient method depends on integrating over potential energy functions which provide a useful description of energies even near the top of the potential well, a region where the vibrational-rotational energy levels are not readily accessible. This makes this method particularly useful for predicting high-temperature properties outside the range of laboratory measurements and beyond the useful limits of the partition function approach. In the present work, we use the virial coefficient method to predict the heat capacities and enthalpies of nitrogen up to 25,000 K.
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  • 86
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    International journal of thermophysics 11 (1990), S. 597-601 
    ISSN: 1572-9567
    Keywords: measurement techniques ; nitrogen ; parallel-plate apparatus ; thermal conductivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A parallel-plate apparatus is suited for accurate measurements of the thermal conductivity coefficient of fluids over a wide range of densities. This is illustrated by measurements of the thermal conductivity coefficient of nitrogen at a temperature of 308.15 K and at pressures up to 20.1 MPa with an accuracy of 0.5%. The agreement with a recent correlation based on accurate measurements by other authors is satisfactory.
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  • 87
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    International journal of thermophysics 11 (1990), S. 897-910 
    ISSN: 1572-9567
    Keywords: binary mixtures ; carbon dioxide ; cylindrical resonator ; electrostatic transducers ; ethane ; gas ; isotherm ; methane ; mixtures ; multicomponent ; mixtures ; natural gas ; nitrogen ; propane ; sound speed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A description of a fixed-path length acoustic resonator which uses electrostatic transducers for sound generation and detection is given. Also, a summary of the measurements on 13 binary and 4 multicomponent gas mixtures of natural gas components is given. Data were obtained at pressures to 10 MPa for five isotherms at 25 K increments from 250 to 350 K. The binary mixtures are primarily methane-rich, with either ethane, nitrogen, carbon dioxide, or propane as the second constituent. The multicomponent mixture compositions represent four naturally occurring natural gas mixtures.
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  • 88
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    Biodegradation 10 (1990), S. 309-328 
    ISSN: 1572-9729
    Keywords: estuaries ; groundwater ; nitrogen ; nitrogen cycle ; nitrogen loading
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We examined the importance of nitrogen inputs from groundwater and runoff in a small coastal marine cove on Cape Cod, MA, USA. We evaluated groundwater inputs by three different methods: a water budget, assuming discharge equals recharge; direct measurements of discharge using bell jars; and a budget of water and salt at the mouth of the Cove over several tidal cycles. The lowest estimates were obtained by using a water budget and the highest estimates were obtained using a budget of water and salt at the Cove mouth. Overall there was more than a five fold difference in the freshwater inputs calculated by using these methods. Nitrogen in groundwater appears to be largely derived from on site septic systems. Average nitrate concentrations were highest in the region where building density was greatest. Nitrate in groundwater appeared to behave conservatively in sandy sediments where groundwater flow rates were high (〉 11/m2/h), indicating that denitrification was not substantially reducing external nitrogen loading to the Cove. Nitrogen inputs from groundwater were approximately 300 mmol-N/m3/y of Cove water. Road runoff contributed an additional 60 mmol/m3/y. Total nitrogen inputs from groundwater and road runoff to this cove were similar in magnitude to river dominated estuaries in urbanized areas in the United States.
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  • 89
    ISSN: 1573-5087
    Keywords: Lemna gibba ; cytokinins ; isopentenyladenosine ; zeatinriboside ; abscisic acid ; nitrogen ; phosphorus ; relative growth rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relationship between endogenous cytokinin content and relative growth rate (RGR) was studied in cultures of Lemna gibba L. G3 supplied with daily doses of mineral nutrients that were increased exponentially over time. At the optimal level of nutrient supply the RGR was 30–35% day-1. The RGR was regulated by adjusting the rate of nitrogen supply, or it was restricted by addition of 0.5 μM abscisic acid (ABA). Another approach used to investigate the specific roles of nitrogen (N) and phosphorus (P), was to transfer optimally growing plants to media without N or P but otherwise complete. The plants were harvested at regular intervals for determination of the RGR and levels of cytokinins of the isopentenyladenosine (iPA) and zeatinriboside (ZR) types with an enzyme-linked immunosorbent assay (ELISA). Levels of both iPA- and ZR-type cytokinins decreased when nitrogen was applied to cultures in growth limiting amounts. The cytokinin levels decreased more rapidly than the RGR when either N or P was lacking in the medium, suggesting an early influence of nutrient availability on cytokinin levels which in turn may induce adaptive response by the plant. RGR retardation induced by ABA did not affect cytokinin levels during the first 4 days of the treatment, and the later effects were small. The experiments gave no indication that ABA is involved in the adaptation response of Lemna plants to nutritional stress.
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  • 90
    ISSN: 1573-5117
    Keywords: phytoplankton ; primary production ; phosphorus ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Lake Erken climatic factors such as duration of ice cover, snow-depth and insolation govern the phytoplankton development and the species composition during the spring, with significant variations from year to year. Generally the small diatom, Stephanodiscus hantzschii var. pusillus creates a conspicuous peak at ice-break. In some years motile dinoflagellates start to develop under the ice already in early March, which results in a much longer spring bloom. The highest biomasses were recorded in 1954–1955 with values up to 11 mg 1−1 of fresh weight. The chlorophyll a concentrations have at most reached an epilimnetic average of 30 µg 1−1. The primary production reached a maximum value of 2200 mg C m−2 d−1 in 1955 and the average production for two months during the spring varied from 30 to 64 mg C m−3 d−1. Concerning nutrients, phosphorus was shown to be the limiting nutrient at the end of the spring bloom. This fact was confirmed by orthophosphate concentrations, algal surplus phosphorus content and alkaline phosphatase activity, as well as estimations of inorganic N : P and C : P ratios and nutrient enrichment experiments
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  • 91
    ISSN: 1573-5117
    Keywords: culture ; macroalga ; nitrogen ; nutrition ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seaweeds have physiological mechanisms to acquire, utilize, and store various forms of nitrogen in environments where nitrogen levels vary tremendously in space and time. Knowledge of the nitrogen relationships of seaweeds is required for the development of successful seaweed mariculture. For example, it would seem at first that continuous nitrogen enrichment would be desirable in such systems because maximal seaweed yields are possible only when growth is not nitrogen-limited. Yet such fertilization is wasteful and can result in yield reductions due to the enhancement of epiphyte growth. Because most seaweeds can rapidly taken up high concentrations of nitrogen, far in excess of what is required for current growth demands, enrichments are needed only when internal nitrogen concentrations fall to near the critical level (i.e., the minimal tissue concentration of nitrogen required for maximal growth). Nutrients are best applied at brief pulses of high nitrogen concentrations.
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  • 92
    ISSN: 1573-5117
    Keywords: phosphorus ; alkaline phosphatase activity ; phytoplankton ; Cyanobacteria ; nitrogen ; correspondence analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Alkaline phosphatase activity (APA) was investigated monthly for 4 years in the eutrophic Lake Nantua, a lake colonized by a large population of Cyanobacteria. Total enzymatic activity as well as specific activities (the ratios between APA and biomass expressed as dry weight, chlorophyll a, cell phosphorus) varied strongly but they followed a similar pattern during each year. The data were processed using correspondence analysis. Specific APA was never related to depth but highest activities were always associated with low particulate phosphorus and nitrogen, low dissolved inorganic phosphorus (DIP) concentrations, low chlorophyll a to filament number ratio and zero nitrate in the waters, indicating P and N limiting conditions. However a high N/P ratio, close to Redfield optimum also occurred at these conditions. Low activities were associated only with high chlorophyll a to filament number ratio. The results suggest that, during summer P-depletion and as long as the N/P ratio is close or above an optimum value, the DIP enzymatically regenerated from DOP pool by phosphatases could temporarily contribute to the algal phosphorus supply.
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  • 93
    ISSN: 1573-5079
    Keywords: Glycine max L. ; photosynthesis ; ribulose 1,5-bisphosphate carboxylase/oxygenase ; ribulose 5-phosphate kinase ; senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The abundances of ribulose-1,5-bisphosphate carboxylate/oxygenase (Rubisco) and ribulose-5-phosphate (Ru5P) kinase in field-grown soybean (Glycine max L. Merr.) leaves were quantified by a Western blot technique and related to changes in chlorophyll and photosynthetic capacity during senescence. Even though the leaf content of Rubisco was approximately 80-fold greater than that of Ru5P kinase, the decline in the levels of these two Calvin cycle enzymes occurred in parallel during the senescence of the leaves. Moreover, the decrease in the content of Rubisco was accompanied by parallel decreases of both the large and small subunits of this enzyme but not by an accumulation of altered large or small subunit isoforms. With increasing senescence, decreases in abundances of Rubisco, Ru5P kinase and chlorophyll were closely correlated with the decline in photosynthetic capacity; thus, the specific photosynthetic capacity when expressed per abundance of any of these parameters was rather constant despite an 8-fold decrease in photosynthetic capacity. These results suggest that during senescence of soybean leaves the chloroplast is subject to autolysis by mechanisms causing an approximately 80-fold greater rate of loss of Rubisco than Ru5P kinase.
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  • 94
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    Photosynthesis research 25 (1990), S. 187-197 
    ISSN: 1573-5079
    Keywords: excessive light ; photoprotection ; photosynthesis ; photosystem II regulation ; xanthophyll cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The possibility that zeaxanthin mediates the dissipation of an excess of excitation energy in the antenna chlorophyll of the photochemical apparatus has been tested through the use of an inhibitor of violaxanthin de-epoxidation, dithiothreitol (DTT), as well as through the comparison of two closely related organisms (green and blue-green algal lichens), one of which (blue-green algal lichen) naturally lacks the xanthophyll cycle. In spinach leaves, DTT inhibited a major component of the rapidly relaxing high-energy-state quenching' of chlorophyll fluorescence, which was associated with a quenching of the level of initial fluorescence (F′0) and exhibited a close correlation with the zeaxanthin content of leaves when fluorescence quenching was expressed as the rate constant for radiationless energy dissipation in the antenna chlorophyll. Green algal lichens, which possess the xanthophyll cycle, exhibited the same type of fluorescence quenching as that observed in leaves. Two groups of blue-green algal lichens were used for a comparison with these green algal lichens. A group of zeaxanthin-free blue-green algal lichens did not exhibit the type of chlorophyll fluorescence quenching indicative of energy dissipation in the pigment bed. In contrast, a group of blue-green algal lichens which had formed zeaxanthin slowly through reactions other than the xanthophyll cycle, did show a very similar response to that of leaves and green algal lichens. Fluorescence quenching indicative of radiationless energy dissipation in the antenna chlorophyll was the predominant component of ‘high-energy-state quenching’ in spinach leaves under conditions allowing for high rates of steady-state photosynthesis. A second, but distinctly different type of ‘high-energy-state quenching’ of chlorophyll fluorescence, which was not inhibited by DTT (i.e., it was zeaxanthin independent) and which is possibly associated with the photosystem II reaction center, occurred in addition to that associated with zeaxanthin in leaves under a range of conditions which were less favorable for linear photosynthetic electron flow. In intact chloroplasts isolated from (zeaxanthin-free) spinach leaves a combination of these two types of rapidly reversible fluorescence quenching occurred under all conditions examined.
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  • 95
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    Plant and soil 128 (1990), S. 115-126 
    ISSN: 1573-5036
    Keywords: ammonium ; mineralization ; nitrate ; nitrification ; nitrogen ; roots ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tomato root growth and distribution were related to inorganic nitrogen (N) availability and turnover to determine 1) if roots were located in soil zones where N supply was highest, and 2) whether roots effectively depleted soil N so that losses of inorganic N were minimized. Tomatoes were direct-seeded in an unfertilized field in Central California. A trench profile/monolith sampling method was used. Concentrations of nitrate (NO3 -) exceeded those of ammonium (NH4 +) several fold, and differences were greater at the soil surface (0–15 cm) than at lower depths (45–60 cm or 90–120 cm). Ammonium and NO3 - levels peaked in April before planting, as did mineralizable N and nitrification potential. Soon afterwards, NO3 - concentrations decreased, especially in the lower part of the profile, most likely as a result of leaching after application of irrigation water. Nitrogen pool sizes and rates of microbial processes declined gradually through the summer. Tomato plants utilized only a small percentage of the inorganic N available in the large volume of soil explored by their deep root systems; maximum daily uptake was approximately 3% of the soil pool. Root distribution, except for the zone around the taproot, was uniformly sparse (ca. 0.15 mg dry wt g-1 soil or 0.5 cm g-1 soil) throughout the soil profile regardless of depth, distance from the plant stem, or distance from the irrigation furrow. It bore no relation to N availability. Poor root development, especially in the N-rich top layer of soil, could explain low fertilizer N use by tomatoes.
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  • 96
    ISSN: 1573-5036
    Keywords: anaerobic soils ; bacterial flush ; mineralisation ; nitrification ; nitrogen ; restoration ; soil perfusion ; soluble carbon ; stockpiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Topsoil stockpiled for 4 years resulted in an accumulation of NH4-N at depths of 1m or more in mound, as measured by an ammonia gas-sensing electrode. When leached with water these soils were also found to contain high concentrations of dissolved organic C below 1m. Both NH4-N and DOC were products of microbial mineralisation of soil organic matter that accumulated under anaerobic conditions. When these soils were restored a flush of decomposition took place, fuelled by labile organic matter and soluble nitrogen. Stockpiled soil which underwent an ammonium-rich perfusion regime in the laboratory indicated that in-mound soils rapidly attained greater nitrification potential than surface mound soils and also had greater potential for further mineralisation of organic matter to NH4-N. This further production was seen as a contribution from the bacterial flush, stimulated by the large labile-C pool already present. As the bulk of stored soil was anaerobic, restored soils were seen as potentially wasteful of their N-reserves; the fate of nitrogen and soluble carbon compounds in restored soils is discussed.
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  • 97
    ISSN: 1573-5036
    Keywords: Citrus aurantium L. ; photosynthesis ; VA Mycorrhizae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sour orange (Citrus aurantium L.) grown in low-P (9–12 ppm) and high-P (420 ppm) soil inoculated with or without Glomus intraradices (G.i.), were evaluated for biomass, carbohydrates, ribulose bisphosphate carboxylase (RuBPCase), phosphoenolpyruvate carboxylase (PEPCase) activity, leaf 14CO2 incorporation, and other physiological parameters. Growth of plants in the low-P, noninoculated soil was lowest, with total dry biomass reduced up to half of the low-P, inoculum treatment. Total nonstructural carbohydrates were 40% lower in leaves of plants in the low-P, noninoculated soil, compared with the other treatments. Inoculation of the low-P soil enhanced leaf 14CO2 incorporation by 67%, total chlorophyll content by 28%, and RuBPCase activity by 42%, compared with low-P, noninoculated treatment. Improved P-use efficiency by G.i. in low-P soil was comparable to high-P nutrition in improving leaf 14CO2 incorporation and concentration of major leaf photosynthetic products that include starch and sucrose. Leaf PEPCase activity in the low-P, noninoculated treatment, however, was at least threefold higher than the other treatments, suggesting a possible alteration in organic acid metabolism in sour orange leaves as a result of P deficiency.
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  • 98
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    Plant and soil 129 (1990), S. 219-225 
    ISSN: 1573-5036
    Keywords: drying ; grassland ; heating ; microbial biomass ; mineralization ; nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A dwarf bamboo-type grassland soil (Thick High-humic Andosol) was nitrogen-limited for grass despite the presence of a considerable amount of microbial biomass N. By either treatments of air-drying and subsequent heating, the content of mineral N in the soil was increased by 3.7 g N and 11.7 g N m-2, respectively, after a 55-day incubation period. The efficiency of mineralized N for growth of orchardgrass was compared with nitrate-N added just before cultivation. The dry matter content of the grass increased from 81.7 g (control) to 169 g and to 337 g m-2 in the dried and in the heated soils, respectively, when N application was omitted. Of the mineral N released by air-drying and heating of the soil, 84% and 77% were absorbed by the grass, and 30% and 20% was assumed to be derived from microbial biomass, respectively. In contrast the grass apparently absorbed 54–56% of the 5 g nitrate-N m-2 added to the control and the air-dried soils. It was also noted that fungal biomass N had decreased by 1.5–1.9 g m-2 in the control soil after addition of 10 g nitrate-N m-2.
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  • 99
    ISSN: 1573-5079
    Keywords: CA1P ; CO2 fixation ; enzyme regulation ; photosynthesis ; rubisco ; RuBP carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Metabolism of 2′-carboxy-D-arabinitol 1-phosphate (CA1P) is an important component in the light-dependent regulation of ribulose-1,5-bisphosphate carboxylase (Rubisco) activity and whole leaf photosynthetic CO2 assimilation in many species, and functions as one mechanism for regulating Rubisco activity when photosynthesis is light-limited. Species differ in their capacity to accumulate CA1P, ranging from those which can synthesize levels of this compound approaching or in excess of the Rubisco catalytic site concentration, to those which apparently lack the capacity for CA1P synthesis. CA1P is structurally related to the six carbon transition state intermediate of the carboxylation reaction and binds tightly to the carbamylated catalytic site of Rubisco, making that site unavailable for catalysis. Under steady-state, the concentration of CA1P in the leaf is highest at low photon flux density (PFD) or in the dark. Degradation of CA1P and recovery of Rubisco activity requires light and is stimulated by increasing PFD. The initial degradation reaction is catalyzed by an enzyme located in the chloroplast stroma, CA1P phosphatase, which yields carboxyarabinitol (CA) and inorganic phosphate as its products. The pathway of CA metabolism in the plant remains to be determined. Synthesis of CA1P occurs in the dark, and in Phaseolus vulgaris this process has been shown to be stimulated by low PFD. The pathway of CA1P synthesis and its relationship to the degradative pathway remains unknown at the present time. The discovery of the existence of this previously unknown carbon pathway in photosynthesis indicates that we still have much to learn concerning the regulation of Rubisco activity and photosynthesis.
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  • 100
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    Photosynthesis research 24 (1990), S. 167-173 
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; photosynthesis ; Dunaliella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relaxation of the non-photochemical quenching of chlorophyll fluorescence has been investigated in cells of the green alga Dunaliella following illumination. The relaxation after the addition of DCMU or darkening was strongly biphasic. The uncoupler NH4Cl induced rapid relaxation of both phases, which were therefore both energy-dependent quenching, qE. The proportion of the slow phase of qE increased at increasing light intensity. In the presence of the inhibitors rotenone and antimycin the slow phase of qE was stabilised for in excess of 15 min. NaN3 inhibited the relaxation of almost all the qE. The implications of these results are discussed in terms of the interpretation of the non-photochemical quenching of chlorophyll fluorescence in vivo and the mechanism of qE.
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