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  • 1987  (116)
  • 1
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    Plant systematics and evolution 155 (1987), S. 45-48 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Inuleae ; Phagnalon ; Ph. rechingeri spec. nova ; Taxonomy ; Flora of Iran
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phagnalon rechingeri spec. nova from S Baluchestan (Iran) is described as a species new to science and illustrated; its relationships to other species of the genus, in particular to thePh. woodii group from S Arabia, are discussed.
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  • 2
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    Plant systematics and evolution 155 (1987), S. 49-54 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Chenopodiaceae ; Noaea griffithii ; Salsola griffithii ; Taxonomy ; sand vegetation ; psammophytes ; Flora SW Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Noaea griffithii, which hitherto was known from the type collection only, is transferred toSalsola by reason of its horizontal embryo and other fruit characters. The description is completed; ecological, phytocoenological and distributional data are given. The species is a common endemic psammophyte of SE Iran, S Afghanistan and adjacent Pakistan Baluchistan.
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  • 3
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    Plant systematics and evolution 155 (1987), S. 27-43 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Cyperaceae ; Carex ; Endemics ; floristical connections ; Flora Iranica area ; Flora of: Iran ; Afghanistan ; Iraq ; Turkemenia ; Pakistan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 88 species and 20 subspecies ofCarex, altogether 94 taxa, occur in the area ofRechinger's Flora Iranica. Of these, 48 taxa, have an Euro-Siberian, 11 an Irano-Turanian and 35 a Central Asiatic distribution, the last including five species with connections to E. and tropical SE. Asia. The Saharo-Sindian element is missing. Endemic taxa are found in all groups. Eight taxa, endemic to the NW. Himalayas occur in the E. part of the area. The Euro-Siberian taxa are concentrated in the NW. of the area, but have connections to the east; similarly, Central-Asiatic taxa are concentrated in E. Afghanistan and N. Pakistan, but have connections to the west. Some Euro-Siberian taxa are widely distributed in the area, but avoid the most arid regions. The Irano-Turanian taxa are few and extend from Egypt through the area of Flora Iranica to Kazakhstan SSR and Central China.
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  • 4
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    Plant systematics and evolution 155 (1987), S. 15-25 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Cousinia ; Generic distribution map ; centres of diversity ; distribution patterns ; Flora of the Irano-Turanian Region ; Flora of SW. Asia ; of Iran ; Iraq ; Afghanistan ; Pakistan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract On the basis of new floristic works a distribution map of the genusCousinia is given and discussed. The generic distribution area ofCousinia is nearly identical with the Irano-Turanian Region, but most of the 662 species are concentrated on the Iranian and Turkestanian mountain regions. Here, both in the eastern and in the western parts of the distribution area, four centres of diversity with high numbers of—mostly endemic—species are found: The western Tienshan (61 species), Pamiro-Alaj (169), NE-Afghanistan (80), NW-Afghanistan (44); Kopetdagh (66 in Iran, 33 in Turkmenia), Elburz (66), the northern part of Zagros (44), Azerbaijan (36). The outlines of the generic area are formed by genetic-historical and by climatic factors. The distribution patterns ofCousinia species confirm the close connections between the Iranian and the Turkestanian mountains as centres of origin and conservation of palaeo-xeromorphic mountain floras, contrasting with the Turanian lowland flora.
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  • 5
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    Plant systematics and evolution 155 (1987), S. 67-69 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Caryophyllaceae ; Acanthophyllum ; Diaphanoptera ; Ochotonophila ; Scleranthopsis ; Capsule dehiscence ; Flora of Iran ; Afghanistan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The shape and the mode of dehiscence of the capsule had been regarded as good differential characters betweenAcanthophyllum and related genera.—Studies of these characters, including the shape of the ovary, in species ofAcanthophyllum, Diaphanoptera, Ochotonophila andScleranthopsis show, however, that they cannot be used as differential characters for the genusAcanthophyllum.
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  • 6
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    Plant systematics and evolution 155 (1987), S. 71-75 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rubiaceae ; Rubieae ; Galium ; G. subfalcatum ; spec. nova ; G. campylotrichum ; spec. nova ; G. tetraphyllum ; spec. nova ; Flora of Pakistan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three new species ofGalium from the NW. Himalaya in Pakistan are described and illustrated.
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    Plant systematics and evolution 155 (1987), S. 77-87 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rubiaceae—Rubieae:Galium gymnopetalum ; spec. nova ; G. lahulense ; spec. nova ; G. saipalense ; spec. nova ; G. nepalense ; spec. nova ; G. serpylloides Royle exHook. f. typified ; Alpine flora of the Himalaya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Galium serpylloides agg. comprises a group of locally endemic, disjunct and vicarious taxa in the (sub)alpine zone of the W. Himalaya, from W to E:G. gymnopetalum, G. lahulense, G. serpylloides s. str.,G. saipalense, andG. nepalense. Four of these are new to science;G. serpylloides is newly typified. The main characters of the five species are compared and illustrated, their affinities and evolution are discussed, and a map of distribution is presented.
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  • 8
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    Plant systematics and evolution 155 (1987), S. 93-103 
    ISSN: 1615-6110
    Keywords: Angiosperms ; new taxa ; Arenaria ; Campanula ; Scutellaria ; Stachys ; Calamintha ; Aristolochia ; Allium ; Flora of Turkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 10 new Turkish taxa are described:Arenaria eliasiana, A. sivasica, A. monscragus, A. angustifolioides; Campanula lycica; Scutellaria orientalis subsp.tortumensis; Stachys choruhensis, S. tundjeliensis; Calamintha caroli-henricana; Aristolochia rechingeriana, the latter two species named in honour ofKarl Heinz Rechinger;Allium vuralii.
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  • 9
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    Plant systematics and evolution 155 (1987), S. 89-92 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Hypericaceae ; Hypericum ; H. dogonbadanicum ; H. socotranum subsp.smithii ; Chorology ; phytogeography ; Flora of Iran ; Socotra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypericum dogonbadanicum Assadi, a local species from theQuercus brandtii forests of the Zagros Mountains in south-western Iran, belongs to sect.Campylosporus (Spach)R. Keller and is most closely related to a Socotran endemic,H. socotranum Good subsp.smithii N. Robson. Despite the inclusion of these forests in the Irano-Turanian Element of the Iran flora byZohary (1963),H. dogonbadanicum appears to belong rather to the Mediterranean dwarf-shrub maquis type of community and therefore to be a member ofZohary's (1963) Mediterranean/Irano-Turanian group of Iranian species.—The key position of the Socotran endemics to the understanding of the inter-sectional relationships is discussed.
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  • 10
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    Plant systematics and evolution 155 (1987), S. 105-132 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Scrophulariaceae ; Veronica ; Veronica agrestis group ; V. bungei ; V. ceratocarpa ; V. persica ; V. polita ; V. siaretensis ; V. francispetae ; V. agrestis ; V. opaca ; V. filiformis ; Allopolyploidy ; significance of polyploidy ; neophytes ; anthropochory ; migration ; evolution of weeds ; Flora of Iran ; SW. Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A character analysis reveals a clearly intermediate position of the tetraploidV. persica (2n = 28) between the two diploid speciesV. polita andV. ceratocarpa (both 2n = 14) which are morphologically rather different and have been placed by several authors in different sections of the genus.V. ceratocarpa is native to subhumid deciduous forests of the Caucasus and of the Elburz mountains (N. Iran);V. polita has its centre of variation in the Elburz range where it grows in therophyte habitats. Three other closely related species,V. bungei, V. siaretensis, andV. francispetae, are endemic to the Elburz range which is the main centre of diversity and variability of theV. agrestis group. This comprises all the above mentioned species and also two more European weeds:V. agrestis andV. opaca. Veronica polita, was probably originally native to open places in deciduous mountain forests, before becoming a weed in neolithic times and migrating to Europe; nowadays it has an almost world-wide distribution. The allotetraploidV. persica combines the ecological characters of its parents, the slightly xerophyticV. polita and the more mesophyticV. ceratocarpa, thus being preadapted to become a highly successful weed with a large ecological range. It has spread rapidly almost all over the world since the early 19th century.
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  • 11
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    Plant systematics and evolution 155 (1987), S. 165-187 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Guttiferae ; Hypericaceae ; Growth form ; vicarious taxa ; nomenclature ; Flora of the Mediterranean ; of Greece ; of Crete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphology, altitudinal distribution and phytocoenology ofHypericum empetrifolium Willd., var.oliganthum Rech. fil. and var.tortuosum Rech. fil. are reassessed and as a result, the taxa are treated at subspecific rank. Essential characters proved to be growth form, number of flowers, foliage and epidermal surface characters, shape of seeds and testa sculpturing. The different growth forms can be interpreted as adaptations to the local habitats.Hypericum empetrifolium Willd. subsp.empetrifolium is a low subshrub of various vegetation types known as phrygana whereasHypericum empetrifolium Willd. subsp.oliganthum (Rech. f.)Hagemann grows as a cushion-like dwarf shrub in chasmophyte associations rich in relic species.Hypericum empetrifolium Willd. subsp.tortuosum (Rech. f.)Hagemann is confined in the high mountain regions and grows as a true dwarf espalier shrub with low spreading shoots.
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  • 12
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    Plant systematics and evolution 155 (1987), S. 189-195 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Anacardiaceae ; Pistacia lentiscus var.chia. ; Taxonomy ; mastic production ; Flora of Greece
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The author discusses the history of an unusual form ofPistacia lentiscus L. (referred to as var.chia (Desf. exPoiret) DC. on the basis of literature data and personal field observations on Chios island. As a result of incisions made on the trunk and stems the tree exudes a specific resinous gum called mastic. The production of mastic currently amounts to 160–170 tons per annum and plays an important role in the economy of the island constituting the main source of income for approx. 20 villages in the south of Chios. The history of mastic dates back 2500 years to the time ofHerodotus. The author points out that on the plantation only male individuals are cultivated; these have been selected over a long period based on mastic yield. As they represent a group of cultivated clones it would be preferable to consider them as a collective cultivar ‘Chia’.
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    Plant systematics and evolution 155 (1987), S. 197-213 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Lamiaceae (Labiatae) ; Thymus sect.Teucrioides ; Th. teucrioides ; Th. rechingeri spec. nov. ; Th. leucospermus spec. nov. ; Taxonomy ; Mountain flora of Greece
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheThymus teucrioides Boiss. & Spruner aggregate is revised and the following new taxa, all from the alpine zone in the Greek mountains, are described:Th. leucospermus Hartvig from the calcareous mountains of Pindhos and Mt Parnassos in Sterea Ellas,Th. rechingeri Hartvig with the subsp.macrocalyx Hartvig from calcareous mountains in Sterea Ellas and N Peloponnissos, andTh. teucrioides subsp.alpinus Hartvig from the serpentine areas of N Pindhos. In the variableTh. teucrioides s. str. many characters have turned out to be markedly geographically correlated and many local populations can be distinguished by a particular combination of characters.
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  • 14
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    Plant systematics and evolution 155 (1987), S. 215-217 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Fabaceae ; Onobrychis aliacmonia ; Endemism ; threatened plants ; rediscovery ; Flora of Greece (Makedhonia, Peloponnisos)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Onobrychis aliacmonia was discovered in 1956 and described in 1973, the year when it was last collected. Its single known locality was flooded soon afterward by an artificial lake now extending over the middle portion of the Aliakmon Valley (western Greek Makedhonia), and the species was believed extinct. It has now been rediscovered in the southern Peloponnisos, in an area far south of the locus classicus and in a widely different ecological context.
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    Plant systematics and evolution 155 (1987), S. 251-256 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Caryophyllaceae ; Silene ; S. fabaria ; S. thebana ; S. ionica ; New combination ; Flora of Greece
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Silene thebana Orph. exBoiss. is transferred as a subspecies toS. fabaria (L.)Sm., resulting in the new combinationS. fabaria (L.)Sm. subsp.thebana (Orph. exBoiss.)Melzh. The chromosome count of 2n = 24 is recorded for the first time for this taxon.
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  • 16
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    Plant systematics and evolution 155 (1987), S. 219-249 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Lamiaceae ; Scutellaria rubicunda ; S. rupestris ; S. brevibracteata ; Biosystematics ; experimental crosses ; morphological variation ; genetic differentiation ; Flora of Greece
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Strict sterility barriers where found between theScutellaria populations on Sicily and the Greek populations. The material from Samos is separated by a strict sterility barrier from the other Greek populations. In contrast, the populations on mainland Greece and adjacent islands are all ± interfertile. Three species are recognized,S. rubicunda Hornem., endemic to Sicily, andS. brevibracteata subsp.icarica, endemic to Samos and Ikaria. All other populations are referred toS. rupestris with eight subspecies, most of which are endemic to one island or to one mountain. Three new subspecies are described, viz. subsp.rechingeri and subsp.olympica, endemic to mt Vourinos and mt Olympus in North Greece, respectively, and subsp.caroli-henrici, native to the Malea peninsula of Peloponnisos. The phytogeographical connections and genetic differentiation within and between populations are discussed.
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    Plant systematics and evolution 155 (1987), S. 277-282 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Bromus fasciculatus subsp.delilei. ; Callus shape ; leafsheath indumentum ; Near East and Mediterranean flora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The syntypes ofBromus madritensis var.delilei Boiss. comprise two different elements: specimens ofB. haussknechtii Boiss. and ofB. fasciculatus C. Presl s.l. By its lectotypification and on the basis of new morphological characters, the validation ofB. fasciculatus subsp.delilei (Boiss.)H. Scholz 1971 was substantiated. This subspecies represents the eastern marginal segregate of the south-Mediterranean species.B. fasciculatus var.alexandrinus Thell. is a minor variant of the typical subspecies.
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  • 18
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    Plant systematics and evolution 155 (1987), S. 257-276 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Boraginaceae ; Pulmonaria ; P. dacica s.l. ; P. mollis s.l. ; P. rubracarnica-stiriaca-vallarsae group ; P. australis s.l. ; Paraskevia ; P. cesatiana ; Polyploidy ; dysploidy ; diploid ; tetraploid and new triploid ; hypo- and hypertriploid species ; chorology ; phylogeny ; Flora of Eurasia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Comparative morphological, karyological and chorological studies prove the distinctness of the SE European and Asiatic populations ofPulmonaria mollis s.l., to be treated asP. dacica (Figs. 4, 5).P. mollis s.str. is interpreted as a relatively young taxon, which probably has evolved from an older stock ofP. dacica ancestors during the Pleistocene (Figs. 6, 8) and might have migrated, perhaps with oak woodland communities, from SE to C. Europe. A similar evolution can be postulated for S—SE European dysploidP. rubra-carnica-stiriaca-vallarsae group; this may have involved still unknown tetraploids (P. carnica?, Fig. 7). This group is linked via the hypertriploid speciesP. vallarsae with theP. saccharata group and at least the eastern species of theP. australis group. The monotypic genusParaskevia apparently marks the earliest divergence from the common Tertiary ancestral stock (Fig. 8). It exhibits a tetraploid chromosome number (2n = 28) but has preserved the most primitive characters. — Some comments on the systematics and nomenclature ofP. australis andParaskevia are added.
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  • 19
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    Plant systematics and evolution 155 (1987), S. 311-318 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Zygophyllaceae ; Fagonia ; F. kassasii ; F. bruguieri ; F. schimperi ; F. olivieri ; Flavonoids ; chemosystematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seven flavonol glycosides were identified from the main taxa of theF. bruguieri complex. Of these, kaempferol 3-rhamno-galactoside, Quercetin 3-rhamnogalactoside and Quercetin 3-galactoside are new records for the genusFagonia L. and theZygophyllaceae s. str. The distribution of these flavonoid glycosides is discussed with respect to the morphology, chemosystematics, and possible phylogeny of the complex and the genus.
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    Plant systematics and evolution 155 (1987), S. 283-306 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Geraniaceae ; Geranium ; New hybrids ; interspecific fertility level as index of relationship ; autopolyploidy ; allopolyploidy ; asymmetry in reciprocal crossings ; Hogenboom's theory of incongruity ; climatic influence on crossing success
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cross-pollinations were carried out among 11 briefly described species ofGeranium. Eight species pairs produced hybrids, of which five had not been reported before. The close relationship ofG. purpureum, G. robertianum andG. rubescens (sect.Ruberta) was confirmed; they form a polyploid series (diploid, tetraploid and octoploid on base x = 16). ForG. canariense (sect.Anemonifolia), another octoploid on base x = 16, the results suggest greater affinity with the former section.G. maderense andG. palmatum of sect.Anemonifolia (2n = 68) are confirmed as closely related to each other.G. maderense produced hybrids withG. robertianum (2n = 64; sect.Ruberta) and withG. cataractarum (2n = 36; sect.Unguiculata). Meiosis in the latter hybrid suggests allopolyploidy between parents with 2n = 32 and 2n = 36. Whereas all these species clearly form a very close alliance,G. lucidum (sect.Lucida) andG. macrorrhizum andG. dalmaticum (both sect.Unguiculata), appear genetically more isolated from them. One plant ofG. macrorrhizum ×G. dalmaticum was raised. — In crosses where hybrids or non-germinating seeds resulted, the reciprocal cross in the majority of cases produced a greatly inferior result or none at all. This asymmetry of response could in some cases be explained by inability of pollentubes from short-styled parents to reach the ovary of a long-styled species and in others by a modification of Hogenboom's theory of incongruity, but neither explanation works for every case. In all our asymmetric results where the ploidy level differed the diploid was the successful female, not the tetraploid, as is usually the case. — Some variation in results from year to year could be attributed to weather conditions.
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    Plant systematics and evolution 155 (1987), S. 319-332 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Orchidaceae ; Habenaria ; Flora of New Guinea and Australia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 5 new taxa ofHabenaria, namelyH. bougainvillae, H. elongata R. Br. var.leptophylla, H. ensigera, H. rechingeri andH. trichoglossa, are described and illustrated, with reference to affinities to related Australian and Indo-malayan species. The occurrence in New Guinea of severalHabenaria spp. typical for a savanna-like vegetation, led to look more thoroughly at these taxa:H. elongata R. Br. andH. ochroleuca R. Br., considered so far to be endemic in Northern Australia, andH. khasiana Hook. f., hitherto only known from southeastern Asia.
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    Plant systematics and evolution 155 (1987), S. 333-337 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Geraniaceae ; Pelargonium sect.Hoarea ; P. caroli-henrici spec. nova ; Taxonomy ; Flora of South Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pelargonium caroli-henrici spec. nova (sect.Hoarea) is described. The new species is apparently endemic to quartzite areas of Vanrhynsdorp Division in the western Cape Province (South Africa). The chromosome number 2n = 22 was counted on specimens in cultivation.
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    Plant systematics and evolution 155 (1987), S. 339-347 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Beaufortia ; Chamaelaucium ; Drosera ; Pultenaea ; Flora of Australia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four new species of the generaBeaufortia, Chamaelaucium, Drosera, andPultenaea are described based on the author's collections from SW. Australia. Their affinities are discussed and full illustrations are provided.
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    Plant systematics and evolution 155 (1987), S. 349-354 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Lentibulariaceae ; Pinguicula lignicola ; P. casabitoana ; Heteromorphy ; Flora of Cuba
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    Topics: Biology
    Notes: Abstract Some new morphological features of the endemicPinguicula lignicola of Cuba are discussed, a detailed description of the heteromorphic habit and illustrations of this epiphytic species are presented. The identity of the other member of sect.Discoradix, P. casabitoana from Española, is considered.
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    Plant systematics and evolution 156 (1987), S. 1-4 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Moraceae ; Ficus ; Nuclear DNA contents ; speciation ; woody habit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nuclear DNA contents in 15 species of large tropical hardwood genusFicus have been determined by cytophotometry. The 2 C-values are rather low and uniform, suggesting no appreciable changes during speciation. The small genome size is discussed in relation to woody habit.
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    Plant systematics and evolution 156 (1987), S. 5-11 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Caryophyllaceae ; Silene latifolia ; S. alba ; S. pratensis ; Geographic variation in pollen morphology ; clinal variation ; detrended correspondence analysis ; character incongruence ; pollen dimorphism
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    Notes: Abstract Reticulate and microechinate pollen forms withinSilene latifolia (S. alba, S. pratensis) were first described from N. America, where the species is an introduced weed. A previous study showed that the two forms also exist in Europe and intergrade along a zone roughly congruent with intergradation zones in seed morphology and flavone glycosylation genotypes. The present survey of pollen from herbarium specimens is more extensive and covers localities from nearly the whole of the species' native Eurasian and North African range. The first axis of a detrended correspondence analysis (DCA) of qualitative characterstate data for 11 pollen morphological characters shows a gradient between the two extreme exine types, reticulate and microechinate. When mapped, the co-ordinates of pollen samples on this axis indicate a broad zone of intergradation which coincides approximately with the somewhat sharper transition between low- and high-tubercle seeds in C. and N. Europe, but becomes diffuse in Italy and the Mediterranean region and diverges from the seed transition zone in SW. Asia and the Middle East. The biological significance of the pollen morphs is unknown; the variation pattern in pollen morphology, unlike that in seeds, is not consistently correlated with macroclimate.
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    Plant systematics and evolution 156 (1987), S. 29-42 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Lactuceae ; Scorzonera ; Karyology ; karyosystematics ; chromosome morphology ; evolution ; Flora of the Iberian Peninsula
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    Notes: Abstract A karyological study of 15 taxa ofScorzonera L. from the Iberian Peninsula has been made. The chromosome numbers found inS. hispanica var.pinnatifida, S. baetica, S. reverchonii, S. angustifolia, S. laciniata var.calcitrapifolia and var.subulata (2n = 14) are new. Diploid cytotypes with 2n = 14 and 2n = 12 prevail, andS. hispanica var.crispatula is the only taxon which exhibits autopolyploidy (2n = 14, 28). x = 7 is considered to be the base chromosome number within the genus, with x = 6 being derived from it by translocation. This and detailed karyotype analyses allow to group the Iberian Peninsula species ofScorzonera into three groups.
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  • 28
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Ligularia calthifolia ; Araceae ; Acorus calamus ; Evolution ; phylogenetic trees ; 4.5 S rRNA ; 5 S rRNA ; chloroplasts
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    Notes: Abstract Chloroplast 4.5 S rRNAs of the monocotAcorus calamus and the dicotLigularia calthifolia have been sequenced. Phylogenetic trees for the chloroplast 4.5 S and 5 S rRNAs and also for cytosol 5 S rRNAs have been constructed by several methods. They are compared with previous studies. Evidently, it is necessary to consider the inequality of nucleotide substitution rates in different lines for adequate phylogenetic reconstructions. Some relevant conclusions are presented. The possibilities and prospects for using data on low-molecular-weight rRNAs from cytosol and organelles for deducing phylogenetic relationships in plants are discussed.
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    Plant systematics and evolution 156 (1987), S. 55-59 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Potamogetonaceae ; Hydrocharitaceae ; seagrasses ; Zostera marina ; Z. noltii ; Posidonia oceanica ; Cymodocea nodosa ; Halophila stipulaceae ; Chromosome numbers ; cytotaxonomy
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    Notes: Abstract The chromosome numbers of the five European seagrasses have been determined in material from several sites along the coasts of the Atlantic Ocean, the North Sea and the Mediterranean:Zostera marina L., 2n = 12;Z. noltii Hornem., 2n = 12;Posidonia oceanica (L.)Delile, 2n = 20;Cymodocea nodosa (Ucria)Aschers., 2n = 14, 2n = 28;Halophila stipulacea (Forsk.)Aschers., 2n = 18. The difference in chromosome morphology betweenZ. marina andZ. noltii supports the division of the genus into two subgenera.
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    Plant systematics and evolution 156 (1987), S. 61-67 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Cucurbitaceae ; Citrullus lanatus ; C. colocynthis ; C. ecirrhosus ; Praecitrullus fistulosus ; Acanthosicyos naudinianus ; Isozymes ; seed protein electrophoresis ; phylogeny ; domestication
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    Notes: Abstract Electrophoretic analysis of 26 enzyme coding genes was conducted on accessions of threeCitrullus species and the relatedPraecitrullus fistulosus andAcanthosicyos naudinianus. The isozyme phylogeny of the genusCitrullus and the related species was constructed based on pairwise measurements of the respective genetic distances between the species and races.P. fistulosus andA. naudinianus form two distinct outgroups toCitrullus which is characterized by two main clusters: The first includes twoC. colocynthis races and the second,C. lanatus andC. lanatus var.citroides, which are more closely related to each other than they are toC. ecirrhosus. The isozyme phylogeny is consistent with the variability in six seed protein bands and with the crossability relations among the examined species.
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    Plant systematics and evolution 156 (1987), S. 91-115 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Neottieae ; Limodorinae ; Cephalantherinae ; Neottinae ; Listerinae ; Pollination ; evolutionary trends ; phylogenetics of orchids
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    Notes: Abstract The various classifications of the orchid tribeNeottieae are reviewed and a new classification is proposed that divides the tribe into three subtribes,Neottiinae, Limodorinae, andCephalantherinae, based primarily on characters of the column (gynostemium). A cladistic analysis illustrates that these three subtribes are more closely related to one another than either is to any other group in subfam.Neottioideae, although there are very few apomorphic characters for the tribe. Pollination biology is also discussed showing links between breeding systems and distribution. There is also a possible role between column and labellum morphology and the emergence of a deceptive pollination syndrome from one of reward.
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    Plant systematics and evolution 156 (1987), S. 69-89 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Valerianaceae ; Xylem transfer cells
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    Notes: Abstract Stems, incl. rhizomes, and roots of 42 species ofValerianaceae were investigated in order to reveal the occurrence, structure and distribution of xylem transfer cells. Within nodes and internodes their frequency, distribution and gradients of development are similar to other families. — Within the secondary xylem of some species transfer cells can develop from cambial derivates, inValeriana tuberosa andPatrinia villosa even from pith cells. Within the turnip ofV. tuberosa transfer cells are very frequent and well developed. Here, after degradation of the cell-wall ingrowths they can be redifferentiated into storage cells which usually contain starch grains (“Hüllenstärkekörner”). In the transitional zone between stem and root of some predominantly herbaceous taxa transfer cells are often very frequent and form large protuberances before they degrade and lignify. SEM observations inValeriana decussata show that the cell-wall ingrowths are degradated at the beginning of lignification with the exception of brush-like protuberances remaining in the half-bordered pit-pairs. During the subsequent process of lignification the simple pits of a wall adjacent to a vessel can be transformed into corresponding pit-pairs. In this case the residues of the protuberances within the pit chamber can be transformed into incrustations similar to the vestures of bordered pits described byBailey (1933). Structural similarities between the brush-like protuberances in the half-bordered pits of theValeriana transfer cells and the ingrowths found inLauraceae (Castro 1982, 1985) are evident. Supposedly, all the cambial derivatives inValerianaceae can develop protuberances at least within their pits. Thus, it appears possible to interpret the vestures of the bordered pits as rudimentary protuberances, and to suggest that they have a specific function in the selective transport of solutes.
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    Plant systematics and evolution 156 (1987), S. 117-126 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Papilionoideae ; Vigna minima ; Polymorphism ; floral breeding systems ; pollination
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    Notes: Abstract Four types of floral breeding systems—(i) chasmogamy, (ii) aerial pseudocleistogamy, (iii) subterranean pseudocleistogamy and (iv) obligate subterranean true cleistogamy—are observed in the populations ofVigna minima inhabiting the Western Ghats (India). Five categories of phenotypes are recognized based on the number and kinds of floral breeding systems found in a given individual. The frequencies of different categories of phenotypes not only show intra- and interpopulation variation, but also fluctuate from generation to generation suggesting differences in the genetic structure of populations. This polymorphism in the breeding system of a single species is unique and may be adaptive. Obligate subterranean true cleistogamy and amphicarpy appear to be adaptations to jungle fires and soil erosion.—The flowers are of the flag-blossom type and insect visitors act as tripping agents. The tripping mechanism together with the polymorphic floral breeding system result in a balanced mixture of selfing and outcrossing. Such a recombination system may enhance the fitness ofV. minima which is essentially a colonizing species.
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    Plant systematics and evolution 156 (1987), S. 133-141 
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    Keywords: Angiosperms ; monocotyledons ; Eriocaulaceae ; Moldenkeanthus ; Paepalanthus ; Leiothrix ; Syngonanthus ; Systematics ; flower morphology
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    Notes: Abstract The genusMoldenkeanthus has to be rejected; its two species have to be placed inPaepalanthus Kunth. The supposed differential character of fused filaments and petals as well as the fusions between petals and stigmas are due to erroneous observations. A revised and emended description ofPaepalanthus itremensis (Morat)Stützel comb. nov. andPaepalanthus bosseri (Morat)Stützel comb. nov. is presented. Furthermore, it is shown that the generaPaepalanthus andLeiothrix also can be distinguished by characters of the gynoecium even when appendices are lacking.
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    Plant systematics and evolution 156 (1987), S. 127-131 
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    Keywords: Angiosperms ; Scrophulariaceae ; Orobanchaceae ; Lesquereuxia = Siphonostegia syriaca ; Parasitism ; secondary haustoria ; primary haustorium ; apical meristem ; evolution
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    Notes: Abstract In the parasiticScrophulariaceae andOrobanchaceae, two types of contact organs exist: secondary and primary haustoria. Secondary haustoria are lateral organs, developing in large numbers and only when the seedling is fully established. In contrast, a primary haustorium represents the first developmental stage of the seedling itself. In the root system of the parasiticLesquereuxia syriaca (=Siphonostegia syriaca) there are only secondary haustoria, but a few of them apparently develop in a terminal position. This is achieved by transferring the haustorial initiation region closer to the root apex. One can interpret this as a transformation of the apical meristem into a meristematic haustorial tissue. On the condition that an extreme shortening (abbrevation) of the primary root could happen, we discuss the transformation of the terminal secondary into a primary haustorium.
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    Plant systematics and evolution 156 (1987), S. 143-150 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Cyperaceae ; Pseudomonads ; Juncus-variation ; cotyledonary sheath
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    Notes: Abstract Aspects of the life history ofKyllinga monocephala are described. Anther wall development corresponds to the Monocot type. The endothecium shows spiral thickenings. The tapetum is glandular and has uninucleate cells. Ubisch granules are present. Mature pollen grains (pseudomonads) are 3-celled at maturity. Ovules are bitegmic, crassinucellate and develop a funicular obturator. The embryo development conforms to theJuncus-variation of the Onagrad type. Endosperm, seed coat and pericarp are described.
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    Plant systematics and evolution 156 (1987), S. 151-157 
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    Keywords: Angiosperms ; Lythraceae ; Lythrum salicaria ; Evolution ; heterostyly ; pollen ; gender specialization
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    Notes: Abstract Some of the factors governing seed-set in three populations ofLythrum salicaria are examined during two seasons. Plants spatially segregated from legitimate neighbours by distances exceeding 1m set few seeds. Plants with small inflorescences set fewer seeds than those with medium or large inflorescences. Flowers having self pollen applied to the stigma before legitimate pollination set fewer seed per flower than those pollinated with legitimate pollen only. Seeds of the mid-style morph germinated more effectively than those of the long style morph and seeds of the short-style morph showed very low levels of germination.
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    Plant systematics and evolution 156 (1987), S. 183-188 
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    Keywords: Angiosperms ; Poaceae ; Psathyrostachys ; Hybrids ; meiosis ; C-banding patterns
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    Notes: Abstract Hybrids between the Chinese endemic speciesPsathyrostachys huashanica Keng and the SW. Asian speciesP. fragilis (Boiss.)Nevski (all 2n = 14) developed normally but were completely sterile. Meiotic analyses revealed a high chiasma frequency indicating that the two species as well asP. juncea (Fisch.)Nevski share the same basic genome (called N). The hybrid nature of the plants was established through karyotype analysis and Giemsa C-banding.
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    Plant systematics and evolution 156 (1987), S. 197-206 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Cruciferae ; Dolichorhynchus arabicus ; genus et spec. nov. ; Arabidopsis erysimoides ; spec. nov. ; Flora of Saudi Arabia
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    Notes: Abstract A new genus ofCruciferae, Dolichorhynchus Hedge & Kit Tan (D. arabicus Hedge & Kit Tan) and a new speciesArabidopsis erysimoides Hedge & Kit Tan, both from northern Saudi Arabia, are described and illustrated. An addendum lists new Crucifer generic or species records for the Arabian peninsula.
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    Plant systematics and evolution 156 (1987), S. 189-195 
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    Keywords: Angiosperms ; Papaveraceae ; Papaver somniferum subsp.somniferum ; subsp.setigerum ; P. glaucum ; P. gracile ; Triploidy ; crossing relationships ; meiotic chromosome pairing ; F2 chromosome number segregation
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    Notes: Abstract Results obtained from crossing experiments betweenP. somniferum subsp.somniferum (2n = 22) and subsp.setigerum (2n = 44),P. glaucum (2n = 14) andP. gracile (2n = 14) and from the observation of meiotic chromosome pairing in the various hybrids obtained do not provide straightforward evidence for the hypothesis thatP. somniferum originated as a triploid hybrid between taxa similar toP. glaucum andP. gracile (Kadereit 1986a, b).—On the one hand, the pattern of crossability found reflects the closer similarity of subsp.somniferum toP. glaucum and of subsp.setigerum toP. gracile, which was interpreted as segregation of parental characters, and the high frequency of 2n = 28 chromosomes among F2-progeny from the hybrid subsp.somniferum × subsp.setigerum (2n = 33) might reveal n = 7 as the base number also ofP. somniferum. On the other hand, however, the general difficulty of obtaining hybrids, and the low incidence of bivalent formation in their meiosis, probably indicating a lack of chromosome homology between the different species, do not fit the above hypothesis.—These results are in marked contrast to the morphological similarity between the three species involved.
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    Plant systematics and evolution 157 (1987), S. 1-7 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Onagraceae ; Corsinipollenites oculusnoctis parvus ; Fossil pollen grains ; pollen grain ultrastructure ; viscin threads
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    Notes: Abstract Fossil onagraceous pollen grains from two Upper Miocene localities in E. Austria were investigated by LM and EM. Exine structure and sculpture as well as viscin threads suggest affinities with the extant genusCircea.
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    Plant systematics and evolution 157 (1987), S. 9-31 
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    Keywords: Angiosperms ; Ericaceae ; Rhododendron ; Systematics ; infrageneric classification ; phylogeny ; flavonoids ; anthocyanins ; flower colour
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    Notes: Abstract Recent studies have improved the infrageneric classification ofRhododendron, including my own investigations on flavonoids and anthocyanins as chemosystematic markers. From a synoptical comparison of morphological, anatomical and phytochemical characters a new system for the genus is proposed. Phylogenetic character progressions and relationships among subgenera, sections and subsections are discussed and illustrated. Key positions for subg.Candidastrum between chori subgenerumRhododendron andNomazalea, and for subg.Choniastrum between chori subgenerumHymenanthes andNomazalea are suggested.
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    Plant systematics and evolution 157 (1987), S. 33-47 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Secale cereale ; S. montanum ; S. vavilovii ; S. silvestre ; Rye ; evolution ; genetic similarity ; isozymes
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    Notes: Abstract The genetic frequencies of 9 isozyme loci have been estimated in 23 samples of 4 species ofSecale by means of starch gel electrophoresis. The populations ofS. silvestre andS. vavilovii were monomorphic and uniform within each species, those ofS. montanum andS. cereale were polymorphic for most of the isozyme loci. On the basis of isozyme patterns as well as allelic and genotypic frequencies of isozyme loci,S. silvestre can be distinguished fromS. vavilovii, and both fromS. cereale andS. montanum; but there is no clear differentiation between the two latter species. Clusters constructed from genetic distances separateS. silvestre andS. vavilovii, whereasS. cereale andS. montanum were grouped together. The isozymatic data presented here, along with cytogenetic and life habit data, agree with the generally admitted existence of 4 species inSecale, and support the relationships suggested byKhush & Stebbins (1961).
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Mimosoideae ; Acacia terminalis ; Bee- and bird-pollination ; extrafloral nectaries ; intraspecific variation
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    Notes: Abstract Intraspecific variation has been found for several pollination-related characteristics in two isolated populations of the self-incompatible treeAcacia terminalis: floral characteristics including colour and flowering time; style length; size and colour of extrafloral nectaries on the leaf petioles; chemical components of the extrafloral nectar; different taxa of bee pollinators; and frequency differences in bird pollinators. These differences possibly reflect the evolution of two different pollination syndromes within this species.
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    Plant systematics and evolution 157 (1987), S. 63-71 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Alismataceae ; Damasonium ; Breeding system ; autogamy ; protandry ; pollen ; seeds
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    Notes: Abstract The reproductive biology of the genusDamasonium was studied in cultivation and in nature. A correlation was shown between flowering time and latitude. All species are self-compatible but the N. American member of the genus (D. californicum) is strongly protandrous and probably experiences high levels of outcrossing in nature. In the European taxa the stamens and the stigmas mature simultaneously but the levels of inbreeding appear to be greater in the southern species (D. bourgeai, D. polyspermum) than in the northern (tetraploid) speciesD. alisma. The Australian memberD. minus resembles the southern European species in being strongly autogamous and bud-pollination was shown to occur. Floral morphology, stigma exertion, pollen/ovule (P/O) ratio and seed weight each show a strong correlation with the degree of autogamy. Vegetative reproduction occurs only inD. californicum andD. alisma through the production of corm-buds. This study suggests that autogamy inDamasonium is accompanied by a shift in resource allocation from male (pollen) toward female (seed) function. However, further studies are required to assess the quality of seeds and their survivorship in nature.
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    Plant systematics and evolution 157 (1987), S. 73-84 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Coreopsis ; Cladistics ; phylogeny ; chromosome numbers ; flavonoid compounds
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    Notes: Abstract A cladistic study of all 44 species of North AmericanCoreopsis was performed using 35 characters. The resulting cladogram indicated that all 11 sections are monophyletic. At the intersectional level, two lineages were revealed, one consisting of six sections occurring almost exclusively in Mexico and California, and another comprising five sections restricted largely to the eastern and southeastern United States. The cladogram is similar to phylogenies produced by less explicit methods but it differs in two major respects: the monotypic sect.Silphidium is placed with other sections from the southeastern United States rather than with Mexican sections, and sect.Anathysana from Mexico is more closely allied with the three California sections than with sect.Electra from Mexico.
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    Plant systematics and evolution 157 (1987), S. 95-103 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Umbelliferae ; Apiaceae ; Lomatium ; Cladistics ; phenetics ; phylogeny ; sister-taxon
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    Notes: Abstract Lomatium is the largest North American genus ofApiaceae (=Umbelliferae). Relations amongLomatium and other genera have not been well understood. TheApiaceae-Araliaceae lineage and genera of North AmericanApiaceae were analyzed phenetically and cladistically in turn, and seven phenetic alliances of genera are identified. Data on frequencies of character-state combinations were assembled and usingSporne's notions of maximal and minimal co-variation of states, two character-states were identified that are derived among Umbellifer genera. Using cladistic analysis of the alliance consisting ofLomatium and six other genera we identified three sister groups toLomatium:Polytaenia alone;Polytaenia withPseudocymopterus, and a group of four genera. Not all genera in the group of seven withLomatium are supported by autapomorphies impeding phylogenetic reconstructions. Both phenetics and cladistics have helped to suggest and evaluate relationships; to call attention to groups requiring further study, and in particular to identify derived character-states.
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    Plant systematics and evolution 157 (1987), S. 143-159 
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    Keywords: Angiosperms ; Pollen exine sculpturing ; self-incompatibility systems
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    Notes: Abstract The exine morphology and pore size of pollen from diverse angiosperm taxa with homomorphic gametophytic, homomorphic sporophytic, and heteromorphic self-incompatibility have been studied. The results indicate that correlations alleged to exist between the type of self-incompatibility system and the nature of the pollen wall morphology should be treated with considerable caution.
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  • 49
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Hordeum ; sectt.Critesion andAnisolepis ; Isozyme patterns ; kernel proteins ; phylogenetic relationships
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    Notes: Abstract Slab gel electrophoresis techniques have been applied to the study of isozyme and kernel protein patterns in 20 accessions ofHordeum chilense and related species in order to elucidate their phylogenetic relationships. On the basis of our results we can conclude that: (1) Conventional classification based on morphological characters does not totally agree with biochemical data. (2) Sectt.Anisolepis andCritesion seem to be clearly differentiated. (3) The accessions classified asH. compressum present biochemical phenotypes quite different from the rest of the species. (4)H. stenostachys, H. muticum andH. chilense constitute a group of variable species with many biochemical similarities and close phylogenetic relationships. (5) The evolutionary pattern of these American species seems to follow a model of reticulate evolution.
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    Plant systematics and evolution 157 (1987), S. 121-141 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Asteroideae ; Brachyscome (=Brachycome)lineariloba ; B. breviscapis ; Life pattern ; annual inbreeding ; ascending dysploidy ; amphidiploidy ; karyotype ; hybridization ; meiotic pairing ; Flora of Australia
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    Notes: Abstract A comparison of karyotypes ofBrachyscome breviscapis (2n = 8),B. lineariloba cytodemes E (2n = 10), B (2n = 12) and C (2n = 16) suggests that these species have a homoelogous basic set of four chromosome pairs, two large pairs and two small, and that theB. lineariloba cytodemes E, B and C are related toB. breviscapis by successive additions of small chromosomes. A pronounced asynchrony of chromosome condensation between these large and small chromosomes has been observed. In the artificial hybrids betweenB. dichromosomatica (2n = 4) ×B. breviscapis, and theB. lineariloba cytodemes, theB. dichromosomatica chromosomes are similar in size and condensation behaviour to the small chromosomes ofB. breviscapis and ofB. lineariloba cytodemes E, B and C. Meiotic pairing in these hybrids also demonstrates the strong affinities between these chromosomes. It is suggested thatB. breviscapis may be of amphidiploid origin between a species with two large early condensing chromosome pairs and another,B. dichromosomatica-like species with two small late condensing pairs. It seems most likely that the additional small and late condensing chromosomes inB. lineariloba cytodemes E, B and C are derived from theB. dichromosomatica-like parent, and that each addition increases vigour, fecundity and drought tolerance, allowing these cytodemes to colonize more open and arid environments. Transmission of the univalents in the quasidiploidB. lineariloba cytodeme E was verified as being via the pollen, and not via the embryo sacs.
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    Plant systematics and evolution 157 (1987), S. 161-180 
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    Keywords: Angiosperms ; Solanaceae ; Nicotiana ; Nuclear DNA ; heterochromatin ; chromosome differentiation ; evolution
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    Notes: Abstract A survey of 51 species fromNicotiana subgg.Tabacum, Rustica andPetunioides has shown that evolution was accompanied by a five-fold variation in nuclear DNA amounts. This variation, however, was not directly correlated with the changes in chromosome number. Drastic rearrangement of karyotypes is characteristic for the evolution ofNicotiana spp. Significant gain or loss in nuclear DNA has often accompanied such changes, but DNA variation has also occurred without significant changes in karyotype arrangements.—The distribution of nuclear DNA is discontinuous inNicotiana, species cluster into DNA groups with consistently regular increments in the mean DNA amounts. The discontinuities are viewed as “steady states” in terms of genomic balance and biological fitness.—Changes in the amount of nuclear DNA and in the heterochromatin are compared with the morphological, chromosomal and adaptive changes which accompanied speciation in 14 subgeneric sections. The evolutionary significance of DNA variation is discussed.
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    Plant systematics and evolution 157 (1987), S. 181-186 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Capparaceae ; Capparis spinosa ; C. ovata ; Hawkmoths ; Proxylocopa ; Pollination syndrome ; nectar flow
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    Topics: Biology
    Notes: Abstract Two closely related species ofCapparis, C. ovata andC. spinosa, which are exposed to the same pollination environment were studied. The nectar volume and concentration ofC. ovata are higher than inC. spinosa. Both species have similar pollination efficiency, but the relative contribution of each pollinator is different.C. ovata is pollinated mainly hy hawkmoths while the principal pollinators inC. spinosa are bees.
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  • 53
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    Plant systematics and evolution 157 (1987), S. 187-199 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Melastomataceae ; Phyllagathis ; Taxonomy ; inflorescence and floral morphology ; Flora of Malesia and Malaysia
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    Topics: Biology
    Notes: Abstract Phyllagathis tuberculata King and two closely allied new species,P. magnifica A. Weber andP. stonei A. Weber, are described and illustrated. Within the genus, these species form a distinct and isolated group which is restricted to Peninsular Malaysia.P. tuberculata occurs in Perak (probably confined to G. Bujang Melaka),P. magnifica andP. stonei are found in the mountains on the Pahang/Selangor border (Genting Highlands, Gombak valley). The distinctive characters of the three species are listed and some general information relating to inflorescence morphology, tubercle anatomy, fruit structure and seed dispersal is provided.
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  • 54
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    Plant systematics and evolution 157 (1987), S. 201-217 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Liliaceae ; Asphodelaceae ; Bulbine bulbosa ; Heterochromatin ; C-banding patterns ; polyploids ; NORs ; B chromosomes
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    Topics: Biology
    Notes: Abstract C-banding studies support earlier evidence thatB. bulbosa, as a previously circumscribed, is heterogeneous, consisting of three distinct entities: (1) theB. bulbosa complex (B. bulbosa s. str.) at 4x (2n = 24), 8x (2n = 48) and 12x (2n = 72) ploidy levels, (2) the “rock lily” and (3) the Kroombit population (both 2n = 46). Each of these three main groups has a distinctive banding profile, though centromeric and telomeric dot bands, variably expressed, are common to all. In theB. bulbosa complex, substantial heterochromatin development, apart from bands associated with the NORs on chromosomes 1 L, 2 S and 3 L, occurs only at the terminal regions of the short arms of the large and middlesized acrocentric chromosomes, with considerable polymorphic and polytypic variation in the number and size of the heterochromatic blocks, especially at the 4x level. Queensland 8xB. bulbosa populations differ in having terminal heterochromatin, probably associated with NORs, on 11 S and 12 S, and in having some strong interstitial bands. The differences appear to correlate with attributes relating to flower morphology, and may have systematic significance. The karyotypes of “rock lily” and Kroombit are somewhat similar but the former has a characteristic C-band profile with multiple interstitial bands on chromosomes 1–5 and 7–9, whereas the latter has only one interstitial band on chromosome 9.
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  • 55
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    Plant systematics and evolution 157 (1987), S. 219-245 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Hieracium sect ; Pilosellina ; Mode of reproduction ; cytology ; crossing and germination experiments
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    Topics: Biology
    Notes: Abstract Five species are recognized inHieracium subgen.Pilosella sect.Pilosellina Fries. Four are diploid (2x, 2n = 18), one (H. pilosella L.) is highly variable morphologically and cytologically (from 2x to 10x), in its mode of reproduction (self-incompatibility, agamospermy, amphimixis, apo-amphimixis) and in its hybridization pattern. A part of this huge agamic complex was analysed by comparing sexual 4x and apomictic 5x plants (crossing and germination experiments, measurements of vegetative reproduction by stolons etc.). In the experimental garden apomictic 5x produced more stolons than the sexual 4x plants and the total length of the stolons per rosette was greater. However, in nature, the competitive potential of the sexual plants seems to be higher, presumably as a result of the higher mortality of ramets in 5x. Sexual 4x plants often grow in dense and grazed grass vegetation, whereas 5x apomicts often occur in dunes with patchy vegetation. Apomicts produce more capitula per rosette, and sexual rosettes form only about 60% of the number of viable achenes as compared to apomictic ones. Therefore, apomicts appear to be characterized by a greater colonizing ability than sexual plants. Apomictic plants produce equal numbers of viable achenes under conditions of both open pollination and isolation. Sexual plants do not form any viable achenes after isolation and produce a somewhat lower percentage of achenes after open pollination than do apomictics. 5xreproduce exclusively apomictically. Apo-amphimixis was never observed in pentaploids and only very rarely in tetraploids. Addition hybrids are very rare. The cross sexual 4x × apomictic 5x failed in 70% of the attempts, but the recombination of genomes carrying genes for apomixis is possible and results in apomictic 4x and sexual 5x, both with a reduced number of viable achenes. In nature sexual and apomictic plants may occur in close proximity. In such cases the germination rate of the achenes of 4x and 5x is lower; this may indicate that apomictic plants fertilize sexual plants in nature (unidirectional gene-flow). 5x plants form euploid gametes carrying two or three genomes. The results of the crossing experiments can be explained in terms ofNogler's theory of monogenic inheritance of apospory.
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    Plant systematics and evolution 158 (1987), S. 1-10 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Hordeum spontaneum ; Electrophoresis ; morphological and earliness traits ; analysis of variance ; discriminant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Samples from eleven populations of wild barley were examined for metric growth and reproductive traits in a “common garden” field trial. Descendants of these plants were examined for electrophoretically determined genotypes. In most cases each population had one or more predominant electrophoretically detectable genotypes and many infrequent genotypes. Analysis of variance ofHordeum spontaneum shows that the between-population variance component contributed the bulk of the observed variation in metric traits, with only a small proportion of the total variation contributed by the between-genotype within-population variance component. Nevertheless, a full 20% of the F values for the among genotype analysis were significant at the 5% level. In addition, using discriminant analysis, electrophoretically determined genotypes could be easily distinguished on the basis of trait (i.e., metric) measurements. The joint use of electrophoresis (to identify genotypes) and of trait measurements is a powerful tool for investigating intrapopulation genetic variation.
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  • 57
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    Plant systematics and evolution 158 (1987), S. 23-27 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Annonaceae ; Annona ; Duguetia ; Guatteria ; Uvaria ; Pollen ultrastructure ; fragile exines ; systematics of primitive angiosperms
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    Topics: Biology
    Notes: Abstract Besides tectate and columellate, 3-layered exine types, in theAnnonaceae, one also finds very fragile, thin exine types. Their single exine layer corresponds either to a former tectum (including infratectal layer) or a former basal layer. The interpretation of the different origin of the remaining layers is based on their different structure and position within the intine. The fact that reduced exine types are obviously not always homologous should be regarded in systematic interpretations.
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  • 58
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    Plant systematics and evolution 158 (1987), S. 11-22 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Orchidaceae ; Thelymitra antennifera ; Hibbertia ; Pimella ; Goodenia ; Helichrysum ; Stackhousia ; Lasioglossum ; Syrphus damaster ; Eurys ; Pollination ; deceit ; floral mimicry ; Flora of Australia
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    Notes: Abstract Thelymitra antennifera is found to be a general mimic of the search image of other co-blooming yellow or cream-flowered species including members of the following genera:Hibbertia, Goodenia and, to a lesser extent,Helichrysum, Pimelia andStackhousia. The most common pollinators areLasioglossum (Chilalictus) spec. and the introducedSyrphus damaster (hover fly) as well as a solitary wasp,Eurys spec.—T. antennifera acts by deceit, involving opticals (yellow colour and pollen imitation) as well as olfactory (sweet odour) signals. Capsule production is low and density dependent, a situation demonstrated in other species which have evolved pollination syndromes involving deceitful mimicry.
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  • 59
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    Plant systematics and evolution 158 (1987), S. 37-46 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Glycine spp. ; Genome ; adventitious roots ; pod ; taxonomy
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    Topics: Biology
    Notes: Abstract Adventitious roots were observed on 3 wild perennialGlycine species, in short-poddedG. clandestina, G. latifolia and mostG. tabacina (2x, 4x, 6x), while other species lacked adventitious roots. This suggests that the adventitious roots trait is associated with B genome species. Intra- and interspecific F1 hybrids reveal that adventitious roots apparently are inherited recessively. The presence of adventitious roots or short-poddedG. clandestina, coupled with infertility with longer-poddedG. clandestina, and enzymatic and leaflet morphology differences between the twoG. clandestina subgroups, supports segregation of the short-podded form as a separate taxon.
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    Plant systematics and evolution 158 (1987), S. 29-35 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Acanthaceae ; Ruspolia ; Ruttya ; ×Ruttyruspolia ; Intergeneric hybrid ; enzyme electrophoresis
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    Topics: Biology
    Notes: Abstract A plant collected in South Africa in the early 1960's has been considered an intergeneric hybrid with the parental taxa beingRuspolia hypocrateriformis (Vahl)Milne-Redhead var.australis Milne-Redhead andRuttya ovata Harv. The intermediate morphology of the plant provided the strongest evidence of its hybrid origin. The natural hybrid, named formally as ×Ruttyruspolia A. Meeuse & de Wet, is highly sterile. Crosses between the two presumed parental taxa produced two plants that are very similar to the putative natural hybrid. We had examined the presumed parental species and the natural and artificial hybrids using enzyme electrophoresis. The two parental species are highly differentiated at genes specifying soluble enzymes; they have a genetic identity of 0.51. They have no common alleles at two genes, and contain alternative alleles in very different frequencies at two loci.Ruttya andRuspolia exhibit both unique and common alleles at two additional genes. The natural and artificially produced plants of ×Ruttyruspolia are identical electrophoretically and contain alleles unique to each of the parental species at two genes. In addition, individuals of ×Ruttyruspolia combine alternative high frequency alleles from each parent at two loci. Allozymes provide strong confirming evidence for the hybrid origin of naturally occurring ×Ruttyruspolia because the products of specific alleles either unique to or highly characteristic of the two putative parental taxa are found combined in ×Ruttyruspolia.
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    Chromatographia 23 (1987), S. 796-802 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Steroids ; Rat tissue ; Purification by extraction
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    Topics: Chemistry and Pharmacology
    Notes: Summary A high-performance liquid chromatographic method for analysis of steroids in the tissue of laboratory animals is described. A two-stage solid extraction procedure, utilizing Sep-pak C18 and graphitized carbon black, gives a purified product. Steroids in this extract were separated by multistep gradient elution with a reversedphase liquid chromatography system, using acetonitrilewater as solvent. Several columns with different geometry and phase loading were investigated. Optimum resolution was obtained with a medium covered and spherical particle. The applicability of this method was successfully demonstrated in the analysis of the testis and adrenal gland of rat.
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    Chromatographia 23 (1987), S. 814-817 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Thin layer chromatography ; Ammonium tungstophosphate ; Aminoacids
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    Topics: Chemistry and Pharmacology
    Notes: Summary The chromatographic behaviour of aminoacids on columns of silica coated with different percentages of ammonium tungstophosphate at several buffer phosphate concentrations is reported. The stationary phase shows a high selectivity towards aminobutyric acids and methyl derivatives of tryptophan due to the distance between the carboxylic group and the −NH 3 + group involved in the cation-exchange process and/or to the steric hindrance by a methyl substituent. A relationship between HPLC and HPTLC data has been verified and applied to the prediction of the separation of six aminoacids.
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    Chromatographia 23 (1987), S. 934-938 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Calibration ; Confidence interval ; Limit of determination ; Limit of calibration
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    Topics: Chemistry and Pharmacology
    Notes: Summary In HPLC calibration the expressions lowest calibration limit and determination limit are defined in statistical terms. The lowest calibration limit is the minimum mass in the measured series of calibration points. It is calculated from the confidence interval of the inverse of the calibration function as the lowest mass limit that may be differentiated from zero mass with a preset probability of error. If the calculated lowest calibration limit is lower than the actual data, points at lower concentration may be measured. The determination limit is the smallest concentration of an analysis that is differentiated from the concentration zero or an apparent blind value in the calibration curve with a given probability of error. Using two different UV-detectors (variable wavelength and photodiode-array) the lowest calibration limit is experimentally evaluated and compared with specific data for the detectors.
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  • 64
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pre-column derivatization ; Ultraviolet detector sensitivity ; Dihydroartemisinin ; Diacetyldihydrofluorescein
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    Topics: Chemistry and Pharmacology
    Notes: Summary The use of diacetyldihydrofluorescein (DADF) for derivatization of dihydroartemisinin (dihydroqinghaosu, DHQHS) is proposed. The reaction between DHQHS and this reagent in the presence of 4-dimethylaminopyridine (DMAP) and N,N′-dicyclohexylcarbodiimide (DCC) was complete in 8 hours at room temperature giving about 80 per cent theoretical yield. The derivative showed intense UV absorption, thus providing a sensitivity of 0.1 nanogram by UV detection after column separation. The influences of the ratio of the reagents, reaction temperature, chromatographic conditions and the extent of detection linearity were investigated. The reaction gave consistent results and chromatographic separation was not affected by an excess of the reagent or side products.
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    Chromatographia 23 (1987), S. 395-400 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Photokinetic data ; Reaction mechanism ; (E)-1-phenylpropene
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    Topics: Chemistry and Pharmacology
    Notes: Summary A combined method of high-performance liquid chromatography and UV-absorption spectroscopy is described for use in dynamic systems. It provides a way of obtaining detailed mechanistic information as well as quantitative data (rate constants, quantum yields), even though neither mechanism nor photoproducts are known. The kinetic treatment of the photoreaction of (E)-1-phenylpropene was successful, showing a photo-isomerization and a parallel step. Information on this additional step is obtained by using HPLC to observe the changes in the concentrations of the reactants' selectivity. The necessity of process control is demonstrated.
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    Chromatographia 23 (1987), S. 419-422 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fatty acid esters ; Particulate material in seawater
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    Topics: Chemistry and Pharmacology
    Notes: Summary A method for the determination of fatty acids of the molecular weight range of propionic to erucic acid is reported. The acids are converted to either p-bromophenacyl-or p-phenylphenacylesters and the esters are separated by HPLC and detected by UV-absorption. Twentytwo esters, including saturated, monoolefinic and polyunsaturated ones were separated within one HPLC-run. The detection limit is about 5 pmol per fatty acid. The application of the method to particulate material is described.
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  • 67
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Anion-exchange stationary phases ; Polymer coating ; Cross-linking
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    Topics: Chemistry and Pharmacology
    Notes: Summary PHEB=POLY(2-hydroxy, 3N-ethylenediamino)butadiene has been synthesized. This unsaturated pre-polymer can be immobilized on different, preferably inorganic supports of suitable porosity such as silica or alumina by cross-linking. Weak anion-exchange phase are obtained. Such phases do not have the disadvantages of organic polymeric phases, regarding pressure stability and swelling by solvent influence. The ion-exchange capacity and the retentivity of IE-separations can deliberately be varied via the thickness of the cross-linked polymer layer. Alumina can also be coated with PHEB applying the same cross-linking procedure. Such phases exhibit an exceptional chemical stability even when operated with mobile phases at very high pH-values. Separations of excellent selectivity are achieved for inorganic anions, as well as with mixtures of aliphatic and aromatic free acids also including hydroxy- (mono- or poly)carboxylic acids.
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  • 68
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase systems ; Stationary phase-solute interactions ; Polycyclic aromatics
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    Topics: Chemistry and Pharmacology
    Notes: Summary Capacity factors of peropyrene-type polycyclic aromatic hydrocarbons were measured using 15 different liquid chromatographic reversed phase systems. On the basis of the retention data the electric interaction indices have been calculated. Application of these indices for structure-retention studies on peropyrene-type polycyclic aromatic hydrocarbons is demonstrated.
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    Chromatographia 23 (1987), S. 31-32 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Gonadal steroids ; Epimers
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    Topics: Chemistry and Pharmacology
    Notes: Summary Chromatographic separation of biologically active epimeric steroids was carried out using a combination of normal and reversed phases. Testosterone (17β-OH) was separated from its 17α-OH epimer epitestosterone using a normal phase silica column whereas their reduced 5α-metabolites were separated on a reversed phase system. The separation of other gonadal steroids including the epimers 20α- and 20β-hydroxypregn-4-en-3-one is also discussed. The technique is particularly useful for separating mixtures of naturally occurring steroid epimers prior to radioimmunoassay.
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  • 70
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ammonium acetate buffer eluent ; Chlorophylls and degradation products ; Marine phytoplankton
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    Topics: Chemistry and Pharmacology
    Notes: Summary An RP-HPLC procedure which separates chlorophylls and their degradation products is described. By employing an amonium acetate buffered mobile phase and a linear gradient elution, complex mixtures of chloroand phaeopigments are separated in less than 30 minutes. The method has been applied to the control of chlorophyll enzymatic degradation in cultures of two algal species, and has also been successfully used in the analysis of algal xanthophylls.
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  • 71
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Behaviour of metal complexes ; Solvophobic theory
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    Topics: Chemistry and Pharmacology
    Notes: Summary The chromatographic behaviour of Cu(II), Fe(II), Ni(II) and Co(II) complexes with 4-(2-pyridylazo)resorcinol was studied. The experimental results were very sensitive even to less obvious operational parameters like the kind of the cation (sodium or ammonium) of the phosphate salt used as buffer. The puzzling pattern of the data obtained was interpreted in terms of the equations formulated by Horvath's ‘olvophobic” theory which correlates the capacity factors of the solutes with the surface tension of the mobile phase. A satisfactory rationalization of the data was obtained together with qualitative information on the stoichiometry and charge state of metal complexes. Useful suggestions about the direction to address the experimental work in order to obtain the best results was also obtained.
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  • 72
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ligand Exchange ; Diethylenetriaminepentaacetic acid ; Transition metal ion separation
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    Topics: Chemistry and Pharmacology
    Notes: Summary Basic alumina-bonded diethylenetriaminepentaacetic acid (DTPA) has been utilized for the separation and preconcentration of some transition metal ions on the basis of ligand exchange. Breakthrough capacity and rate of sorption have been studied. The distribution coefficients of 16 transition metal ions have been determined in demineralized water, 0.01 M sodium citrate and in four different pH systems. On the basis of differences in Kd values some quantitative separations of metal ions have been achieved. The greater selectivity behaviour (higher Kd values) of the adsorbent for Pt(IV)and Cr(III) has been utilized for their preconcentration in the presence of other metal ions. The method has been employed for the recovery of Pt(IV) and Cr(III) from tapwater and sea-water samples.
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  • 73
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Gel permeation chromatography ; Cheese peptides ; Amino acids
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    Topics: Chemistry and Pharmacology
    Notes: Summary The nitrogen fraction from cheese, soluble in phosphotungstic acid has been analyzed by gel permeation and high performance liquid chromatography. Elution profiles of this fraction on Sephadex G-10 show that there are no peptides of molecular weight higher than 700 daltons. Six fractions have been obtained. The first one has the peptides and some amino acids. Fractions II to VI contains mostly free amino acids. Fraction I has been separated into many peaks by HPLC. Six or seven of these peaks are presumed to correspond to peptides.
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  • 74
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Small-bore packed columns ; Air pollutants ; PAH, nitro and oxy-PAH ; Aldehydes
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    Topics: Chemistry and Pharmacology
    Notes: Summary The preparation of small bore columns (1 mm i.d.) packed with reversed-phase materials is described. High efficiency (Hmin=2 dp), coupled with a small C term (0.005 sec) have been obtained so that high speed can be combined with sufficient resolution for the separation of complex organic mixtures. These columns have been used for the separation of air pollutants dispersed in different matrices (air, particulate matter and rain water). Practical applications include the determination of aldehydes in air and emission samples, PAHs, nitrated and oxygenated PAHs in dust sampled from the stack of an industrial emission or collected in a urban area and the analysis of organic components dissolved in rain water collected at a rural site. Specific detection of these pollutants has been achieved by connecting small bore columns to UV absorbance, voltammetric and fluorimetric detectors. Mass spectra of some specific components have been recorded using the off-line technique.
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    Chromatographia 23 (1987), S. 825-828 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Gasoline ; Single-ring aromatic hydrocarbons ; Polycyclic aromatic hydrocarbons
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    Topics: Chemistry and Pharmacology
    Notes: Summary This work reports a rapid and easy procedure for the analysis of the aromatic fraction in gasoline. No sample pretreatment is required, since the gasoline is diluted in methanol and directly injected into a liquid chromatograph. A spectrophotometer detector and a spectrofluorimeter detector are used in series. The procedure has been applied to a large number of Italian and European refined gasoline samples.
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    Chromatographia 23 (1987), S. 109-111 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Normal and reverse phase ; Aldicarb ; Aldicarb sulfoxide ; Aldicarb sulfone
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    Topics: Chemistry and Pharmacology
    Notes: Summary Aldicarb, aldicarb sulfoxide, and aldicarb sulfone were chromatographed on an octyl-silica bonded-phase column and on an unmodified silica column using acetonitrile/water mobile phases. The elution order of the analytes from the silica column was different from that using the octyl-silica bonded phase and allowed confirmation of residues of aldicarb sulfoxide in citrus nectar. Isocratic elution of the unmodified silica column allowed rapid sample throughput.
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  • 77
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ligand-exchange chromatography ; Chiral bonded phase ; Racemate resolution ; Amino acids
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    Topics: Chemistry and Pharmacology
    Notes: Summary The resolution of racemates on chiral chemically bonded silica gel by high-performance ligand-exchange chromatography is described. The stationary phase was prepared by bonding (−) trans 1,2 cyclohexanediamine to silica gel through the coupling agent 3-glycidoxypropyl silane. The resolution of several α-amino acids and of compounds of pharmaceutical interest is reported.
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    Chromatographia 23 (1987), S. 320-324 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Microbore capillary columns ; Polymer based octadecylsilica bonded phase ; Fat soluble vitamins
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    Topics: Chemistry and Pharmacology
    Notes: Summary Separation behavior of fat soluble vitamins on various chemically bonded materials in non-aqueous reversedphases microcolumn liquid chromatography has been investigated. The best performance was found on a polymer-based octadecyl bonded phase. Optimization of separation for vitamins D2, D3, E and E acetate has been tried using this polymer-based column and it has been found that a binary mobile phase consisting of acetonitrile and methanol gave complete separation of fat soluble vitamins.
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    Chromatographia 23 (1987), S. 331-336 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Microbore columns ; Injection techniques
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary This paper focuses attention on the potentially larger signal-to-noise ratios produced by microbore columns in comparison with conventional columns. The increased chromatographic signals by the application of microbore columns are due to the lower chromatographic dilution of elution profiles which are proportional to the square of the column inner radius. Generally less than 1μl sample should be injected into microbore systems to obtain the full benefit of the column performance. However, since more sample can be loaded on conventional columns compared to microbore columns the advantage of improved signal-to-noise ratio can only be realised in situations where very little sample is available. To inject more than 1μl sample, at the same time avoiding extra band-broadening effects, suitable injection techniques must be available. In this study three injection methods for microbore systems that meet this condition, are studied and compared.
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  • 80
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Anion-exchange chromatography ; Xylenol orange ; Separation of nickel and zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A liquid chromatographic procedure for separating nickel and zinc has been developed. Xylenol orange, which is one of the sensitive and commercially available color-forming agents, was used as a component of the mobile phase. The two ions could be separated on weak anion-exchange gels within 10 min. The procedure was suitable for the determination of nickel and zinc in relatively pure solutions with fairly high sensitivity. The described liquid chromatographic analysis would be also potentially applicable for any aqueous sample containing trace levels of metal cations at 1–10 ppm.
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  • 81
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Oligosaccharides ; Interference by salts ; Complex biological samples ; Enzymatic assays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A method is described for the determination of oligosaccharides in samples of high salt content by RP-HPLC with water as eluent and refractive index detection. The samples, mussel processing wastes which are used as media for microfungus and yeast cultures and hence for enzymatic assays (amilolitic capacity) of these microorganisms that develop within the wastes, have been treated with ethanol to precipitate the macromolecular organic matter and desalted by a modified mixed bed resin before chromatographic analysis is carried out.
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  • 82
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    Chromatographia 23 (1987), S. 579-582 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Backflush technique ; Coal-derived procucts ; Separation of phenolic compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A rapid method was developed for the direct extraction of phenolic compounds from coal-derived products and subsequent isocratic HPLC analysis in a reversed-phase system by using the backflush technique.
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  • 83
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Non-porous gel ; Alkaline-earth cations ; Arsenazo III
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A liquid chromatographic procedure for separating alkaline-earth-metal cations with a non-porous gel column has been developed. Arsenazo III, which is one of the sensitive and commercially available color-forming agents, was used as a component of the mobile phase. Magnesium and calcium could be separated on the nonporous gel column within 55 seconds. The procedure was suitable for the separation of alkaline-earth-metal cations in relatively pure solutions with high sensitivity. Because of its rapidity the described procedure is also potentially applicable for any aqueous sample.
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  • 84
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Biphenols ; Dihydroxyphenylpropanes ; Voltammetric detection ; UV photometric detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The retention behaviour of mono- and dihydroxy derivatives of biphenol and bis(hydroxyphenyl)propanes was studied on chemically bonded C-18, CN-, phenyl, phenoxypropyl- and NH2 stationary phases and on silica gel. The effects of the mobile phase, i.e., of the methanol content, the ionic strength, pH and the contents of cationic and anionic ion-pairing agents on the retention data were investigated. In the reversed-phase systems, all the substances are satisfactorily separated except for 3- and 4-hydroxybiphenyl; these isomers can be separated on silica gel, using a mixture of heptane and propanol as the mobile phase. Sodium dodecylsulphate, present at concentrations higher than the critical micelle concentration (about 10−2 M, depending on the methanol content), causes a decrease in the retention times and an improvement in the separation. UV absorption spectra, calibration curves and the detection limit values were obtained at two wavelengths and at various salt concentrations in the mobile phase. Hydrodynamic voltammograms of the solutes were measured in a carbon fibre detector and the calibration curves and detection limit values were measured at the optimal voltage (+1.2 V/Ag/AgCl) as a function of the mobile phase composition. For most test substances voltammetric detection is two to three times more sensitive than UV detection. The combination of a UV and a voltammetric detector in series has been utilized to identify the products of microbial degradation of biphenyl.
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  • 85
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Thin-layer chromatography ; Ion pairing system ; Log P prediction ; Group contributions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A group of 17 β-carbolines was studied in HPLC and TLC systems in order to predict their partition coefficients (log P values). On account of the basic or acid character of some of these compounds, an ion pairing system gave the best results. Both HPLC and TLC data were comparable for log P prediction but severe pH conditions required the use of TLC plates. Retention data are quantitatively related to lipophilicity (expressed as the Hansch constant) and polarity (as the inductive constant) of the solute molecule.
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  • 86
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chitosan-coated silica gel ; Nucleotides ; Amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new stationary phase was prepared by immobilizing the chitosan, a natural hydrophilic polymer, on microparticolate silica gel. The effect of the polarity, pH and ionic strength of the mobile phase has been studied in order to find optimal conditions for the separation of nucleotides and aminoacids. The influence of the properties of the mobile phase on the retention was examined, allowing to employ the chitosan-coated silica gel both to anion exchange and adsorption, depending on the pH and the polarity of the eluent used.
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  • 87
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase systems ; Phases-solute interactions ; Molecular polarizability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Variations in the electrostatic interaction have been studied using polycyclic aromatic hydrocarbons as standard compounds and different reversed-phase HPLC systems. Various methanol-water and acetonitrile-water binary mixtures were applied as the mobile phase. The stationary phases investigated consist of octadecylsilica and arenyl-silica. Using polarizability, dipole moment and ionization potential values of the solutes and mobile phase components as molecular descriptors an electrostatic interaction model is proposed.
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  • 88
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    Chromatographia 23 (1987), S. 93-96 
    ISSN: 1612-1112
    Keywords: Paper chromatrography ; Column liquid chromatography ; Copper-impregnated zinc silicate ; Separation of amines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Papers impregnated with Cu(II) sorbed on zinc silicate have been used to study the ligand exchange chromatographic behavior of amines. RF values of 16 amines have been studied in 6 different systems. On the basis of differences in RF values various analytically important qualitative separations of amines on impregnated papers and quantitative separations on columns of copper(II) sorbed on zinc silicate have been achieved.
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  • 89
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Linear isotherms ; Octadecyl bonded silica ; Styrene divinylbenzene ; Frontal analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Two reversed-phase LC systems were investigated by frontal analysis for the determination of linear chromatographic conditions, as defined according to the isotherm concept. The Partisil ODS-3 bonded silica and the PRP-1 polystyrene-divinylbenzene resin were used as stationary phases together withtrans-2-hexen-al as test solute and methanol-water mixtures as mobile phases. Particular attention was paid to the respective influence of the two main parameters which may cause sorbent overloading, that is, the capacity factor (k′) and the solute concentration in the mobile phase (Cm). Provided that k′Cm≤10−2 M, linear chromatographic behaviour was observed for both sorbents, the maximum capacities of which were found greater than 1mmolg−1.
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  • 90
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    Chromatographia 23 (1987), S. 189-194 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Instrument bandbroadening by extrapolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Methods to determine the contribution of the chromatographic equipment to the total band broadening which involve replacing the column by a union or a capillary tube are not suitable as they involve a fundamental change in the chromatographic system. The linear extrapolation method, based on the estimation of the relative influence of the instrument variance on solutes with different capacity factors, is a more attractive alternative method since the column remains in the chromatographic system. This method is only valid when a number of conditions are satisfied. By meeting these conditions the error in the instrument variance by using the linear extrapolation method was determined. At the same time, ways to minimise these errors were studied. Use of the linear extrapolation method in combination with conventional columns of 4.6 mm i.d. appears to yield inaccurate results. In combination with microbore columns the method can be used, provided the columns have a maximum length of 5cm and contain a packing material with a particle size of 2 or 3μm. The error in the determined instrument variance is then of the order of 2μl2.
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  • 91
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    Chromatographia 23 (1987), S. 209-214 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Review of triglyceride analysis ; Column temperature gradients ; Combined LC and other techniques
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The present review cites 54 papers dealing with reversephase HPLC analysis of triglycerides. The objective of the review was not merely to list the main contributions made in recent years but also to present a discussion of those papers representing qualitative advances and improvements helping to obviate the difficulties associated with this type of analysis. The use of mobile phase polarity and column temperature gradients and the combination of HPLC with other analytical methods such as mass spectrometry or GLC analysis of fatty acids are examples of such contributions.
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  • 92
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    Chromatographia 23 (1987), S. 254-256 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Synthesis of stationary phases ; Influence of surface pH ; Influence of temperature, concentration and reaction ; time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new way to produce liquid chromatographic stationary phases is suggested. The method is based upon a solide-phase reaction between the silica surface and the adsorbed silane molecules. The role of surface pH is demonstrated. The influence of other reaction conditions (concentration of the silane solution, effect of temperature and reaction time) is discussed.
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  • 93
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chiral stationary phases ; Succinimido carbamate intermediates ; Amino acid enantiomers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Chiral stationary phases (CSPs) for liquid chromatography were prepared by the way of an activated carbamate intermediate. The amino group of aminopropylsilyl silica gel was first activated by carbamylation with disuccinimido carbonate (DSC). The obtained activated carbamate silica gel (ACsil) proved useful as an intermediate for the preparation of urea-type CSPs. The reaction of ACsil with (S)- of (R)-1-(α-naphthyl)-ethylamine gave naphthylethylurea type CSPs. These CSPs were also obtained directly from aminopropylsilyl silica gel by its reaction with optically active (S)- or (R)-succinimido 1-(α-naphthyl)ethyl carbamate (SINEC). Several phenylthiohydantoin amino acid enantiomers and p-bromophenylcarbamyl amino acid enantiomers were resolved on the CSPs by elution with aqueous mobile phase.
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  • 94
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Gas chromatography ; Bonded silica ; Residual silanol groups
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Silanol groups of chromatographic material surfaces were determined by chemical reaction with methyl-lithium. Evolved methane was measured by a gas chromatographic method using a column packed with molecular sieves. This chromatographic technique leads to very reproducible results, particularly for the control of the hydroxylation of a silica gel and for the analysis of residual groups at the surface of bonded silica.
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  • 95
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Aldehydes ; Annular denuder ; Environmental analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An improved procedure for the HPLC determination of C1−C3 aldehydes in environmental samples is described. These air pollutants are quantitatively collected on annular denuders coated with 2,4-Dinitrophenylhydrazine and analyzed as hydrazone derivatives by HPLC with UV absorbance and voltammetric detection. Collection efficiencies of aldehydes are reported and compared with other available methods. Practical examples of the application of the method are given.
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  • 96
    ISSN: 1612-1112
    Keywords: Estriol ; Urine ; Menstrual Cycle ; Column liquid chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high-performance liquid chromatography (HPLC) assay for estriol in nonpregnancy urine is described. After Enzymic hydrolysis, the estriol is extracted from urine by the sorbent trap technique utilizing graphitized carbon black (Carbopack B). After some washing steps, estriol is desorbed by a suitable solvent system. After solvent removal, the sample is injected into an HPLC column for estriol quantification. Analytical recovery of estriol was 96.1%. The precision of the method was 2.6 and 4.9% respectively at 145 and 10.6ng/ml of urine. The limit of sensitivity was set at 0.8 ng/ml of urine. The mean contents of estriol in the follicular and luteal phases were respectively 11.3 and 38.8 ng/ml of urine.
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  • 97
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Isohydric solvents ; Butyrophenones ; Direct-silica gel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The behaviour of butyrophenones in high performance liquid chromatography on silica gel using isohydric solvent systems has been studied. The influence of column temperature, water content, acidity and polarity of isohydric solvents on the retention, column efficiency and resolution of a series of butyrophenones have been investigated. Through the results obtained more insight has been gained into the theoretical aspects of the adsorption process on silica gel with isohydric solvent systems.
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  • 98
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase separation ; Oligodeoxyribonucleotides ; Isolation of pure material
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A reversed-phase chromatographic column suitable for the purification of chemically synthesized large oligodeoxyribonucleotides (oligo-DNA) was prepared. The specifications of this column are; the selected silica (Toyo Soda silica) with large pore size (at least 150 Å) and small particle diameter (5 μm desired) should be grafted only with monochloro alkylating reagent of long alkyl chain (sufficiently C18) so that the carbon content of the resultant packing material is 15–16%. Using this column, we could isolate the targeted large oligo-DNA (up to 50mer) in a large scale (75 μg per one cycle) from the impurities in the reaction mixture formed during the automated synthesis by the phosphite method.
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  • 99
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    Chromatographia 23 (1987), S. 292-300 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Separation of sugars ; Review
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary This contribution to the problems of carbohydrate (sugar) column liquid chromatography, evaluates several currently used systems (reversed phase octadecylated silica gel, amino modified silica gel, cation exchangers on polystyrene and on silica gel basis, polyol derivatised silica gel and anion exchange systems). The elution pattern, analysis time, column efficiency and column life time expectancy are considered to be the important points for this comparison. The application of silica gel-based cation exchanger and of polyol derivatised silica gel is new to this field. The comparison and/or evaluation tries to be critical and to come to a conclusion for the choice of a recommended system.
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  • 100
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Packings of octadecylated microporous glass ; Dimethadione and trimethadione in serum ; Pancreatic stones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The liquid chromatographic determination of trimethadione (TMO) and its methabolite, dimethadione (DMO) were studied by high-performance liquid chromatography (HPLC) on octadecyl-modified microporous glasses, prepared with toluene solution containing octadecyl-dimethylchlorosilane, using two types of microporous glass with various mean pore diameters and/or specific surface areas. Using acetonitrile-water mixtures as eluents, TMO and DMO in human serum were separated on the glasses studied, but with different degrees of resolution. In this present study, we report the development of a rapid and selective HPLC method for the simultaneous analysis of TMO and DMO in human serum.
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