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  • Angiosperms  (27)
  • Autoradiography  (21)
  • Springer  (48)
  • Annual Reviews
  • Elsevier
  • 2005-2009
  • 1990-1994
  • 1985-1989
  • 1980-1984  (48)
  • 1981  (48)
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  • 2005-2009
  • 1990-1994
  • 1985-1989
  • 1980-1984  (48)
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  • 1
    Electronic Resource
    Electronic Resource
    Incorporation of35S-sulphate in chick blastoderms during elongation and during shortening of the primitive streak (1981)
    Springer
    Development genes and evolution 190 (1981), S. 233-236 
    Details
    ISSN: 1432-041X
    Keywords: Sulphate ; Glycosaminoglycan ; Autoradiography ; Gastrulation ; Chick
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chick blastoderms were cultured for 2 h in the presence of35S-sulphate. The distribution of the grains after light microscope autoradiography was compared in blastoderms during the elongation and during the shortening of the primitive streak. A uniform labeling was observed over the cells in both groups. Accumulation of grains was present in both groups at the ventral side of the upper layer, where transmission electron microscope studies have revealed a basal lamina. An additional accumulation of grains occurred over the cells and in the extracellular spaces of the head process and of the rostral part of blastoderms with shortening primitive streaks. This positivity could be correlated with the presence of ingressing and recently ingressed notochordal cells. Treatment of the sections with chondroitinase ABC and/or HNO2 before dipping in the nuclear emulsion demonstrated that at least chondroitin sulphate and N-sulphated heparan sulphate were present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848309
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  • 2
    Electronic Resource
    Electronic Resource
    Synthesis of the intranuclear crystals in Scolopendrium vulgare (L.) SM (1981)
    Springer
    Planta 152 (1981), S. 137-144 
    Details
    ISSN: 1432-2048
    Keywords: Autoradiography ; Intranuclear crystals ; Nucleus, crystolo-Protein transfer-Pteridophytes ; Scolopendrium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Proteinaceous intranuclear crystals are found in the fern Scolopendrium vulgare. During mitosis these crystals are eliminated from the nucleus into the cytoplasm, where they are dissolved. New crystals appear in the nucleus. The site of synthesis of intranuclear crystal proteins was investigated using quantitative ultrastructural autoradiography after incubation with tritiated lysine. The results suggest a migration of cytoplasmic proteins to the nucleus, part of which would then be incorporated into the intranuclear crystals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391185
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  • 3
    Electronic Resource
    Electronic Resource
    Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat (1981)
    Springer
    Cell & tissue research 216 (1981), S. 215-220 
    Details
    ISSN: 1432-0878
    Keywords: Autoradiography ; 3H-proline ; Osteoblasts ; Sympathectomy ; Guanethidine-sulfate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sympathectomy was carried out in rats by injections of guanethidine-sulfate from birth to 14 days of age. At 45 days of age, the activity of osteoblastic cells was monitored by 3H-proline autoradiography. Effectiveness of sympathectomy was verified by light-microscopic examination of superior cervical and celiac ganglia. Grain counts over periosteal osteoblasts of the femoral diaphysis and osteoblasts mesial to the first molar in the mandible demonstrated a significantly reduced uptake of 3H-proline in the sympathectomized rats. The data provide direct evidence of sympathetic influence on osteoblastic activity and suggest that sympathectomy may result in the loss of a trophic influence which is important in the regulation of osteogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234556
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural morphometry of blastogenesis (1981)
    Springer
    Cell & tissue research 215 (1981), S. 643-649 
    Details
    ISSN: 1432-0878
    Keywords: T-lymphocytes ; Blast cells ; Autoradiography ; Electron microscopy ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled with tritiated thymidine. Random samples of cells were taken for light and electron microscopic morphometry. In comparison to the unstimulated lymphocyte, the stimulated cell increased in size and possessed a greater plasma membrane surface area. Increase in cell size was the result of increases in the volumes of all measured subcellular compartments both in the nucleus and the cytoplasm. Heterogeneity of the stimulated cell population precludes firm conclusions regarding the significance of all these ultrastructural changes, though alterations in cell surface are discussed in the context of known biochemical differences accompanying blastogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233538
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  • 5
    Electronic Resource
    Electronic Resource
    Biosystematic studies onCapsella bursa-pastoris (Brassicaceae): Enzyme polymorphism in natural populations (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 73-94 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Brassicaceae ; Capsella bursa-pastoris ; Isoenzyme polymorphism ; population analyses ; geographic variation ; heterozygosity ; breeding system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genetic variability of natural populations ofCapsella bursapastoris in North- and Middle-Europe has been estimated by means of enzyme assays. Zymograms of 81 populations have been developed. 17 loci could be identified, and 8 of them can be heterozygous. Genetic variability is greater between populations than within. No correlation between actual population sizes and genetic heterogeneity could be detected. Some electromorphs shift their frequencies proportionally to increasing adversity of climatic conditions, some appear to be constant over the whole area, and others are characterized by an apparently irregular variation pattern. Marginal populations comprise a significantly higher proportion of heterozygous plants than central ones. Apart from this clinal variation pattern, a mosaic pattern, strongly related to habitat conditions, was observed: genetic heterogeneity is greater in more intensively disturbed sites. The pattern of genetic variation in natural populations ofCapsella bursa-pastoris is rather highly influenced by the breeding system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00983207
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  • 6
    Electronic Resource
    Electronic Resource
    The breeding system ofEmblingia (Emblingiaceae) (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 63-65 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Emblingia ; Emblingiaceae ; Sapindaceae ; Dodonaea ; Stylobasidaceae ; Stylobasium ; Gyrostemonaceae ; Anemophily ; entomophily ; autogamy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Emblingia is a short lived fire weed with a highly autogamous breeding system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00983205
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  • 7
    Electronic Resource
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    Vergleichende cytotaxonomische Untersuchungen anRanunculus seguieri und der Artengruppe desR. alpestris (Ranunculaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 47-60 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Ranunculaceae ; Ranunculus seguieri ; R. alpestris ; R. traunfellneri ; R. bilobus ; R. crenatus ; Relationship betweenR. seguieri andR. alpestris s. lat.: morphology ; crossing experiments ; chromosome numbers ; karyotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphological and cytological investigations as well as crossing experiments were carried out withRanunculus seguieri Vill. and 4 species of theRanunculus alpestris L. group (R. alpestris L.,R. traunfellneri Hoppe,R. bilobus Bertol.,R. crenatus Waldst. & Kit.). ForR. seguieri andR. alpestris, localities and distribution are given in addition to extensive diagnoses. A key to the species includes morphological characteristics and distribution data forR. traunfellneri, R. bilobus, andR. crenatus. New diagnostic characters are described. Crossing experiments betweenR. seguieri and the species of theR. alpestris group were unsuccessful. All 5 species have a chromosome number of 2n = 16, the record forR. bilobus is new. There is no statistically significant difference between the karyotypes ofR. seguieri andR. alpestris s. str. Nevertheless, according to morphological evidence and crossing experiments,R. seguieri is not closely related to theR. alpestris group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984608
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  • 8
    Electronic Resource
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    A chemotaxonomical study of someBoraginaceae: pyrrolizidine alkaloids and phenolic compounds (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 127-134 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Boraginaceae ; Pyrrolizidine alkaloids ; phenolic compounds ; chemotaxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By means of thin layer chromatography pyrrolizidine alkaloids and phenolic compounds in some members of the familyBoraginaceae, subfamiliesHeliotropioideae andBoraginoideae, were studied. FromOmphalodes verna a main alkaloid was isolated with an isoretronecanol (or stereoisomeric) nucleus. The chemotaxonomical model ofTétényi forBoraginaceae based on fatty acids is generally supported, but relationships betweenHeliotropioideae andCynoglosseae appear to be closer, suggesting parallel developments from common ancesters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00983210
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  • 9
    Electronic Resource
    Electronic Resource
    Embryology ofEriocaulon setaceum (Eriocaulaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 175-188 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Eriocaulaceae ; Eriocaulon setaceum ; Embryology ; systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eriocaulon setaceum can be characterized by: young microsporangium wall with epidermis, endothecium (with fibrous thickenings), and glandular tapetum (uninucleate cells); pollen grains 3-celled, spiraperturate; embryo sac development according to the Polygonum type and with antipodal cyst; endosperm nuclear; embryo small, with incipient differentiation into cotyledonary and epicotylary loci; seed coat mainly from the inner layers of the integuments; pericarp 2-layered and membranous. Embryologically, theEriocaulaceae are nearer to theXyridaceae than to otherFarinosae. Their elevation to the rank of an order,Eriocaulales, therefore appears justified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985181
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  • 10
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    Microgeographic edaphic differentiation in allozyme polymorphisms of wild barley (Hordeum spontaneum, Poaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 287-292 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Hordeum spontaneum ; Allozymes ; microgeographic differentiation ; population genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Allozymic variation in proteins encoded by 22 loci was analyzed electrophoretically in 278 individual plants of wild barley,Hordeum spontaneum, the progenitor of cultivated barley, in four 100 meter transects, in Israel, each equally subdivided into basalt and terra rossa soil types. Significant differentiation according to soil was found in 9 alleles. Our results suggest that allozyme polymorphisms in wild barley are at least partly adaptive, and differentiate by edaphic natural selection rather than by stochastic processes, and/or neutrality of allozymic variants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985193
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  • 11
    Electronic Resource
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    Genetic variation inSolanum pennellii: Comparisons with two other sympatric tomato species (1981)
    Springer
    Plant systematics and evolution 139 (1981), S. 11-45 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Solanaceae ; Lycopersicon ; Solanum pennellii ; Allozymes ; electrophoresis ; isozymes ; mating systems ; self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic variation—primarily in 19 genetic loci of seven enzyme systems—was analyzed in accessions from various parts of the geographic range ofSolanum pennellii, which according to all tested biosystematic criteria behaves like a species ofLycopersicon. In comparison with the largely sympatricL. hirsutum andL. pimpinellifolium, this species exhibits the same trends of reduced allogamy and decreased genetic variation toward the north and south margins of its distribution, though to a much lesser degree; it does not exhibit their trends toward smaller flower size in the same peripheral regions. All three species agree to a considerable extent in the ranking of their tested loci in respect to degree of variablility; however, overall polymorphy is highest inS. pennellii. Except for the appearance of self-compatibility at its southernmost margin,S. pennellii is exclusively and rigidly self-incompatible. Alleles are distributed much more uniformly over the range than in the previously mentioned species, marginal and internal endemic mutants being much less abundant. A marked geographic disagreement is evident in regions of high and low variation. These differences in patterns of genetic variability are reconciled in terms of observed differences in mating systems, probable age of distributions, and adaptive strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00983920
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  • 12
    Electronic Resource
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    Eine neueTillandsia-Art aus Südmexico:T. kolbii (Bromeliaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 259-262 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Bromeliaceae ; Tillandsia subgen.Allardtia ; T. kolbii ; spec. nova ; Flora of Mexico
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new species ofTillandsia is described and discussed in regard to related species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985189
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  • 13
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    Ultrastructure of the stylar canal cells ofCitrus limon (Rutaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 263-274 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rutaceae ; Citrus limon ; Style ; canal cells ; canal filling substance ; ultrastructure ; anatomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of the canal cells and the canal filling substance ofCitrus limon have been studied. At maturity the canal cells are very rich in cytoplasm. Their inner tangential walls lining the canal are much thickened and formed by two layers: the outer corresponds to the original wall, the inner is formed by subsequent deposition of abundant materials of different origin. This thickening occurs at the same time as the filling of the stylar canal. Both events are paralleled by considerable dictyosomic activity, the formation of a large amount of rough endoplasmic reticulum, and the incorporation of small cytoplasmic masses into the cell wall, due to plasmalemma evaginations. — The material in the stylar canal has a heterogeneous ultrastructure aspect and consists of polysaccharides, proteins and lipids; it presumably provides nutrients for the growing pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00985190
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  • 14
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    Resistenzphysiologische Grundlagen der evolutiven Kälteakklimatisation von Sproßpflanzen (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 145-180 
    Details
    ISSN: 1615-6110
    Keywords: Coniferophytina ; Angiosperms ; Arecaceae ; Poaceae ; Quercus ; Citrus ; sclerophyllous trees ; mountain plants ; Acclimatation ; chilling resistance ; frost resistance ; stress ecotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gradations in cold resistance of plants with different latitudinal and altitudinal distribution, and the various categories of chilling and freezing resistance are hypothesized to be evolutionary steps of adaptive responses to increasing low temperature stress and annual seasonality. The gradual lowering of the critical phase transition temperature of biomembrane lipids, the capacity of persistent supercooling of tissues, and the development of a dormancy linked freezing-tolerance are considered to be essential mechanisms resulting in improved acclimatation to low temperature climates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989871
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  • 15
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    Differentiation of Fraction 1 protein in relation to age and distribution of Angiosperm groups (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 89-113 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Nicotiana ; Gossypium ; Lemnaceae ; Fraction 1 protein ; differentiation and age of taxa ; migration to Australia ; land connections ; long distance dispersal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fraction 1 protein (F-1-protein) (ribulose bisphosphate carboxy-lase-oxygenase) contained inLemnaceae has been evolving for at least 50 million years because fossils of these plants have been identified in strata belonging to the Upper Cretaceous. Electrofocusing F-1-protein resolves the large subunit polypeptides coded by extranuclear DNA and the small subunit polypeptides coded by nuclear DNA. Four differences affecting isoelectric points of the large subunit polypeptides and eight affecting the small subunit polypeptides are now present among eleven species representing the four genera comprising theLemnaceae. In comparison, four differences in the large and 13 in the small subunit polypeptides exist among 63 species ofNicotiana; four differences in the large and eight differences in the small subunit polypeptides exist among 19 species ofGossypium. The number of differences in F-1-protein composition being of the same order of magnitude for the generaNicotiana, Gossypium, and the familyLemnaceae, we infer that these Angiosperms are of similar antiquity. Nicotiana species indigenous to Australia and Africa contain F-1-proteins whose large subunit polypeptides are different but some of whose small subunit polypeptides are like those found in species from the Western Hemisphere. The same situation is found for the F-1-protein inGossypium. These results are in harmony with the view that species ofNicotiana andGossypium have arrived in Australia via former land connections between S. America, Antarctica, and Australia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984611
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  • 16
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    The tubular exine ofLauraceae andHernandiaceae, a novel type of exine structure in seed plants (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 139-146 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Magnoliidae ; Lauraceae ; Hernandiaceae ; Pollen morphology ; pollen ultrastructure ; Angiosperm phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pollen grains ofLauraceae andHernandiaceae are characteristic in having a tubular exine of ± microfibrillar structure. This is coated with a layer of medium electron-dense globules and very peculiar spines, made up of a substance differing from that of the exine. Such a pollen wall structure is otherwise unknown in seed plants and thus adds to the list of odd features erratically present in various taxa of the Ranalean complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984614
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  • 17
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    Effect of allopolyploidy on the activity of selected enzymes inHibiscus (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 189-198 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Malvaceae ; Hibiscus acetosella ; H. asper ; H. surattensis ; H. radiatus ; andH. cannabinus ; Allopolyploidy ; evolution of dosage compensation ; alcohol dehydrogenase ; malate dehydrogenase ; leucine aminopeptidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mature seeds of diploid and tetraploidHibiscus species were analyzed for enzyme activity (alcohol dehydrogenase, malate dehydrogenase, leucine aminopeptidase), total protein content, DNA amount and dry weight. The recently formed tetraploid,H. radiatus, generally had enzyme and protein levels very similar to the sum of its progenitors, while the more ancient speciesH. acetosella had several lower levels. This difference may reflect the greater amount of timeH. acetosella has had to evolve dosage compensations.
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    URL: http://dx.doi.org/10.1007/BF00985182
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  • 18
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    Genetic determination of pappus part number in the annual hybridMicroseris B 87 (Asteraceae—Lactuceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 235-246 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Lactuceae ; Microseris ; Canalized numbers ; quantitative genetics ; evolutionary genetics ; pappus parts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ChileanMicroseris pygmaea has a ten-part paleaceous pappus while the CalifornianM. bigelovii has five pappus parts on each achene. Hybrids between the two species have between five and ten pappus parts with averages below 7.5. Hybrid B 87 has an F 1 average value of 6.7 pappus parts. 140 F 2 plants were raised from this hybrid, and 12 F 3 families were obtained by selfing from F 2 plants. One larger F 4 family has been raised. Pappus part number in all of these is still canalized between 5 and 10. Variation within these limits is genetically determined by a quantitatively acting polygenic system. Modeling of this system suggests that a minimum of four, but probably not many more, genes are involved. This opens the possibility of a complete genetic analysis of the system.
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    URL: http://dx.doi.org/10.1007/BF00985187
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  • 19
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    Seed and fruit anatomy ofCocculus hirsutus (Menispermaceae) (1981)
    Springer
    Plant systematics and evolution 139 (1981), S. 95-102 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Menispermaceae ; Cocculus hirsutus ; Seed ; fruit ; anatomy ; affinities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ovules ofCocculus hirsutus are anatropous, bitegmic and, crassinucellate. The fruit is drupaceous, black purple, and laterally compressed and has a pericarp demarcated into exocarp and endocarp. The seeds are curved around the basal bodies. Testa and tegmen cells are thin-walled and unspecialized. In the ripe seed the inner epidermis of the tegmen persists, whereas the entire testa and the outer layer of the tegmen degenerate. Relationships of theMenispermaceae toRanunculaceae, Berberidaceae, andLardizabalaceae are supported.
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    URL: http://dx.doi.org/10.1007/BF00983924
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  • 20
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    Der Knotenbau einigerRubiaceae (1981)
    Springer
    Plant systematics and evolution 139 (1981), S. 103-111 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rubiaceae ; Nodal anatomy ; leaf base ; stipules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The leaf base vascularization in most of theRubiaceae corresponds to the unilacunar pattern with one complex trace. Only some species are known to have a trilacunar nodal pattern with three traces. In the unilacunar type one pair of smaller bundles separates from the complex lacunary leaf trace laterally, each soon forking into two arms: One arm becomes a marginal vein of the petiole, the other, besides supplying the stipules, forms a ± distinct vascular “bridge” within the cortex of the nodal flanks. In theRubieae this flank bridge develops as a very distinct vascular “ring” commissure out of which the whorled leaf-like appendages are vascularized; only the opposite true leaves receive their complex trace out of the lacunes directly. Axillary branches originate only from these true leaves.
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    URL: http://dx.doi.org/10.1007/BF00983925
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  • 21
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    Hyphal growth ofHypomyces chlorinus Tul.: An autoradiographic study (1981)
    Springer
    Protoplasma 107 (1981), S. 221-233 
    Details
    ISSN: 1615-6102
    Keywords: Autoradiography ; Cell wall synthesis ; Chitin ; Hypomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The incorporation of the chitin precursor N-acetyl-D-(1-3H) glucosamine byH. chlorinus has been studied by light and electron microscopic autoradiography. Light microscopic autoradiography showed that the incorporation occured preferentially at the hyphal apex. Autoradiograms from electron microscopy were quantitatively evaluated to determine the relative radioactivity incorporation between the cell wall and cytoplasm: this showed that (3H) incorporation took place mainly in the plasmalemma-wall complex. However a small amount of N-acetyl glucosamine can enter into the cytoplasmic space and is then transported by endomembranes (Golgi apparatus-vesicles) to the plasmalemma-cell wall interface before polymerization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276827
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  • 22
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    Sclerorhachis leptoclada (Asteraceae-Anthemideae), eine neue Art aus dem südlichen Khorasan (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 297-299 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae-Anthemideae ; Sclerorhachis leptoclada Rech. f. ; spec. nova ; Flora of Iran
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A fourth species ofSclerorhachis, S. leptoclada Rech. f., has been discovered in S. Khorasan. It differs from the three known species by its tender habit, rosette leaves with few short segments, extremely reduced stem leaves, and very small heads.Sclerorhachis with its four vicarious species confined to the most arid parts of the Iranian highlands is believed to belong to the paleoxeromorphics (Rechinger 1952).
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    URL: http://dx.doi.org/10.1007/BF00985195
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  • 23
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    Heterochromatin and chromosome variation in cultivated species ofTulipa subg.Eriostemones (Liliaceae) (1981)
    Springer
    Plant systematics and evolution 139 (1981), S. 47-55 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Tulipa subg.Liliaceae ; Eriostemones ; Giemsa banding ; heterochromatin ; karyosystematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chromosomes of several cultivatedTulipa species belonging to the subg.Eriostemones were examined using conventional staining and C-banding techniques. Most of the species have lightly banded chromosomes with heterochromatin content varying from nil to about 15%. The banding patterns of several taxa are described and discussed in regard to species relationships.
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    URL: http://dx.doi.org/10.1007/BF00983921
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    Zur systematischen Stellung der GattungProckia: Karyologie und Epidermisskulptur im Vergleich zuFlacourtia (Flacourtiaceae),Grewia (Tiliaceae) und verwandten Gattungen (1981)
    Springer
    Plant systematics and evolution 139 (1981), S. 57-76 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Flacourtiaceae:Prockia ; Flacourtia ; Idesia ; Lacistema ; Rawsonia ; Tiliaceae:Grewia ; Luhea ; Karyomorphology ; epidermis ultrastructure ; Chromosomes and systematics of tropical woody plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Detailed analyses of karyology and leaf morphology do not support relationships betweenFlacourtiaceae andTiliaceae. In spite of different chromosome numbers,Prockia (2n = 18),Flacourtia (2n = 22) andRawsonia (2n = 22) are very similar in karyomorphology, indicating a certain karyological uniformity withinFlacourtiaceae. Lacistema (2n = ca. 62) appears more isolated. On the other hand, theTiliaceae Grewia (2n = 18) andLuhea (2n = 36) have much in common and differ remarkably from the Flacourtiaceous genera. The salicoid leaf-teeth ofProckia are also found inIdesia, but never inTiliaceae. Epidermis ultrastructure reveals certain relationships betweenProckia andFlacourtia in contrast to the strongly differingGrewia. Idesia has a rare und unique epidermis sculpture. — Basic chromosome numbers and chromosomal evolution within theFlacourtiaceae are discussed.
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    Chromosome numbers and rust susceptibility as taxonomic criteria inRosaceae (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 23-38 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Uredinales ; Chromosome numbers ; rust susceptibility ; tribal classification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract BothNeuradoideae andChrysobalanoideae seem rust-free. TheSpiraeoideae andPomoideae are heavily susceptible toGymnosporangium rusts. While thePrunoideae resemble theRosoideae in being vulnerable to attacks ofPuccinia species, they are additionally susceptible toTranzschelia andThekopsora, whereas theRosoideae are characteristically afflicted byPhragmidium.—It is suggested (a) to treat theChrysobalanoideae as a separate family (Chrysobalanaceae), (b) to transferDryas from theRosoideae-Potentilleae-Dryadinae to theRosoideae-Cercocarpeae, and (c) to divide theRosoideae into two main groups of tribes: (i) the rust-freeKerrieae andCercocarpeae with x = 9, and (ii) the rust-susceptiblePotentilleae, Ulmarieae, Roseae andSanguisorbeae with x = 7.
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    URL: http://dx.doi.org/10.1007/BF00983201
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    The evolutionary significance of multigermicity in the genusSpinacia (Chenopodiaceae) (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 57-61 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Chenopodiaceae ; Spinacia ; S. turkestanica ; S. tetrandra ; Reproduction ; multigermicity ; seedball ; dioecy ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The presence of multigerm seedballs in the chenopodiacious genusSpinacia is noted. In the wild, colonising, and “weedy” dioecious species,S. turkestanica andS. tetrandra, the distribution of a multigerm seedball could effectively overcome the problem posed by isolation of the sexes. The hypothesis is tested assessing the extent of intra-seedball progeny hybridisations and seed production in the two wild species. The success in seed production by intra-seedball progeny crosses suggests that the distribution of such seedball progeny groups permits not only a percentage survival under isolation of these dioecious plants, but also the colonisation of areas outside that of the parent populations.
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    URL: http://dx.doi.org/10.1007/BF00983204
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    The embryology ofEpipogium roseum (Orchidaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 1-7 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Orchidaceae ; Epipogium ; E. roseum ; Microsporogenesis ; female gametophyte ; systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development of pollen and female gametophyte inEpipogium roseum (D. Don)Lindl. has been investigated. The embryo sac conforms to the Apinagia type. The taxonomic position ofEpipogium within the family is discussed.
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    Fruit dimorphism inPhaseolus sublobatus (Fabaceae) and its evolutionary significance (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 67-72 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Fabaceae ; Phaseolus sublobatus ; Geocarpy ; amphicarpy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Populations of the annualPhaseolus sublobatus from different ecogeographical zones are genetically differentiated. In twelve populations from the western ghats (Maharashtra range) chasmogamous flowers are arranged in peduncled capitate racemes borne in leaf axils of higher nodes. One population (Poona Race S4) has additional inflorescences, also with chasmogamous flowers, on the main axis between the cotyledonary node and the ground. When the pods of these flowers ripen, the inflorescence gradually coils and, eventually, gets bury the fruits in the soil. This phenomenon is not known in any other plant.—The seed-coat patterns as revealed by SEM, and germination behaviour of both aerial and subterranean seeds are similar, and both types of seeds lack dormancy.—Heavy seed predation selection pressure is probably the principal cause for the evolution of fruit dimorphism inP. sublobatus. The origin of geocarpy in relation to fruit dimorphism and seed predation is discussed.
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    URL: http://dx.doi.org/10.1007/BF00983206
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    Distributional patterns and an additional species of thePassiflora vitifolia complex: Amazonian species diversity due to edaphically differentiated communities (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 95-105 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Passifloraceae ; Passiflora involucrata ; stat. et comb. nov. ; Species β-diversity ; Flora of Amazonia ; Peru
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Field studies in Amazonian Peru show that thePassiflora vitifolia complex, a microcosm of many larger taxa, is concentrated in Amazonia with 4 ecologically separated species. One species is restricted to seasonally inundated forests, a second to non-inundated white sand soils, a third to non-inundated lateritic soils, and the fourth mostly to non-inundated alluvial soils. A new combination is formally proposed to recognize the white sand taxon, previously regarded as a variety, as a distinct species:P. involucrata. The importance of β-diversity in maintaining high Amazonian species diversity is emphasized.
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    The flower biology ofCephalanthera longifolia (Orchidaceae)—pollen imitation and facultative floral mimicry (1981)
    Springer
    Plant systematics and evolution 137 (1981), S. 229-240 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Orchidaceae ; Cephalanthera longifolia ; Cistaceae ; Cistus salviifolius ; Hymenoptera ; Halictus ; Flower biology and ecology ; pollination ; pollen imitation ; floral mimicry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Solitary bees (Halictus sp.) were found to be the effective pollinators ofCephalanthera longifolia. In the same foraging flight the bees also visit flowers ofCistus salviifolius which has a similar colour pattern.Cephalanthera offers no reward to its pollinators, but orange papillae on its labellum successfully imitate pollen ofCistus. AsCephalanthera also attracts pollinators in the absence ofCistus, this is regarded as “facultative floral mimicry”.
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    Investigations on the content of steroidal alkaloids and sapogenins withinSolanum sect.Solanum ( = sect.Morella) (Solanaceae) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 61-71 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Solanaceae ; Solanum sect.Solanum ; Diosgenin ; tigogenin ; solasodine ; chemosystematics ; ontogenetic shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant material of 55 strains from 32 species ofSolanum sect.Solanum ( = sect.Morella) has been investigated for the content of steroidal alkaloids and sapogenins. The leaf-extracts of all species contain diosgenin resp. tigogenin or both together in considerable amount. On the contrary the characteristic steroidal alkaloid solasodine was absent in these samples. For 29 species the presence of steroidal sapogenins has been proved for the first time. In unripe fruits, however, solasodine could be detected in most of the species examined. New occurences of solasodine are reported for seven further species. These chemical results may contribute to a better comprehension of the difficult genusSolanum.—Ontogenetic studies on different organs ofS. atriplicifolium show the relationship between accumulation of steroidal compounds and plant maturity.
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    Tillandsia carminea — eine interessante neue Bromeliacee aus dem Orgelgebirge bei Rio de Janeiro (Brasilien) (1981)
    Springer
    Plant systematics and evolution 138 (1981), S. 293-295 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Bromeliaceae ; Tillandsia subgen.Anoplophytum ; T. carminea ; spec. nova ; Flora of Brazil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tillandsia carminea is a new, morphologically and ecologically distinct species, related toT. stricta Solander, which occurs in the Serra dos Orgaos near Teresopolis, Brazil. It has been taken forT. rosea Lindley, but new investigations have shown thatT. rosea is a synonym toT. stricta and thatT. carminea is a good and distinct species.
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    Histochemical, cytochemical and autoradiographic studies on the rostellum ofHymenolepis diminuta (1981)
    Springer
    Parasitology research 64 (1981), S. 335-345 
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    ISSN: 1432-1955
    Keywords: Hymenolepis diminuta ; Autoradiography ; Ultracytochemistry ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Histochemical studies on the rostellum ofHymenolepis diminuta revealed diastase-stable, protein/neutral carbohydrate-rich material localized in the rostellum tegument. The remainder of the rostellum, primarily composed of the glycogen-rich myocytons of the rostellum musculature, is protein-poor, but rich in diastase-labile, neutral and acidic carbohydrates. Ultrastructural cytochemical studies, using the periodic acid-thiocarbohydrazideosmium (PATCO) technique, indicated that the granules of the rostellar tegumental cytons and distal cytoplasm are carbohydrate-rich. Lipids are present in the rostellar myocytons but not in the tegumental cytons. Autoradiography using a pulse-labeling with [3H]leucine revealed an apical translocation of tegumental granules, but at a slower rate than had been reported for the strobilar tegument of the same organism. Neither [3H]galactose nor [3H]glucose were incorporated into the rostellar tegumental granules. The function of the secretory glycoprotein(s) produced in the rostellar tegument and its (their) possible role in the regulation of maturation and/or strobilization remain enigmatic.
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    Observations on cytoplasmic transport along ovarian nutritive tubes of polyphagous coleopterans (1981)
    Springer
    Cell & tissue research 220 (1981), S. 153-161 
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    ISSN: 1432-0878
    Keywords: Cytoplasmic transport ; Insect ovary ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ovarioles of Coccinella and Tenebrio are shown to be telotrophic — a characteristic normally associated with hemipterans rather than coleopterans. They possess an anterior region of trophic cells and a chain of oocytes. The trophic cells are connected with the latter by a series of nutritive tubes, and autoradiography has shown that RNA is transported along the tubes to the oocytes. However, the system in these beetles differs markedly from that of hemipterans in that the nutritive tubes do not contain an extensive complement of aligned microtubules. The significance of this to both the mechanism and the selectivity of transport is discussed.
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    Bilaterally asymmetrical uptake of [14C]2-deoxyglucose by the octavo-lateralis complexes in flatfish (1981)
    Springer
    Cell & tissue research 214 (1981), S. 659-662 
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    ISSN: 1432-0878
    Keywords: Vestibular system ; Postural control ; Deoxyglucose method ; Autoradiography ; Flatfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary [14C]2-deoxyglucose uptake by neurons located in the octavo-lateralis complex of adult flatfish is asymmetrical on the two sides of the brain. It appears that the neuronal activity on the side oriented upward is higher than that on the side of the brain facing downward. This finding may be significant with respect to the mechanisms of metamorphosis of flatfish and may account for the peculiar fact that these animals swim on one body side during adult live.
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    The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured human small-intestinal tissue (1981)
    Springer
    Cell & tissue research 215 (1981), S. 1-12 
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    ISSN: 1432-0878
    Keywords: Colchicine ; Human enterocyte ; Glycoprotein transport ; Autoradiography ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured biopsy specimens of the human intestine was investigated by light- and electron-microscopical autoradiography and by biochemical methods. The results showed a decrease in the radioactivity of the cell coat on the microvilli and an increase in the Golgi apparatus and in the apical vesicles and tubules. This divergence is attributed to a colchicine-induced impairment of the normal transport of cell-coat glycoproteins from the Golgi apparatus, via the apical vesicles and tubules, to the apex of the cell. The radioactivity of the lysosome-like bodies in the absorptive cells cultured with colchicine also increased. This finding supports a crinophagic function of these organelles in the degradation of excess cell-coat material. The investigations were supported in part by the Foundation for Medical Research (FUNGO), which is subsidized by the Netherlands Organisation for the Advancement of Pure Research
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    Induction by progesterone of a sexual dimorphism of estrogen uptake by anterior pituitary cells in situ (1981)
    Springer
    Cell & tissue research 215 (1981), S. 75-86 
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    ISSN: 1432-0878
    Keywords: Pituitary cells ; Estrogen ; Progesterone ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of progesterone pretreatment on in vivo 3H-estrogen uptake by five anterior pituitary cell types was analyzed by means of a quantitative autoradiographic-immunocytochemical technique. Male and female rats castrated for 14 days show nuclear concentration of label in all five cell types one h after injection of 3H-estradiol, whether progesterone treated or not. The order of labeling intensity is gonadotropes ≧ lactotropes = somatotropes 〉 thyrotropes = corticotropes. Progesterone treatment induces a dramatic sexual dimorphism in estrogen uptake; it significantly increases 3H-estrogen uptake in all female cell types. In males, progesterone decreases uptake in gonadotropes while not altering uptake in other cell types.
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    Monoamine precursor uptake in ganglia of a mollusc Anodonta cygnea L. (Bivalvia) (1981)
    Springer
    Cell & tissue research 219 (1981), S. 403-415 
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    ISSN: 1432-0878
    Keywords: Monoamine precursors ; Ganglia ; Molluscs ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In vitro-uptake and localization of 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine have been investigated by means of light microscopic autoradiography in the central nervous system of the freshwater mussel, Anodonta cygnea L. Accumulation of the labelled monoamine precursors could be observed both over neuronal perikarya and neuropil in the ganglia. 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine were taken up by neurons characterized by a small size (10–20 μm), a polygonal form and an intense staining with toluidine blue. Labelled axon branches situated either in the neuropil or between nerve cells in the cortex exhibited only a moderate activity, frequently of a diffuse character. After pretreatment for 24 h with colchicine, silver grains were also seen over the neuronal perikarya and some axon profiles. This shows that local uptake of the monoamine precursors and, presumably, the synthesis of the monoamines takes place both at perikaryal and axonal/terminal level. The nerve cells accumulating the monoamine precursors contain a nucleus with rich chromatin content and a deeply invaginated nuclear envelope. Further ultrastructural characteristics of these neurons are their well-developed rough endoplasmic reticulum system, a great number of mitochondria and glycogen granules, and the presence of dense-cored vesicles of different types. All these features are responsible for the electron-dense appearance of the cytoplasm.
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    In vitro binding of 125I-HCG to the rat ovary from birth to puberty (1981)
    Springer
    Cell & tissue research 214 (1981), S. 633-640 
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    ISSN: 1432-0878
    Keywords: Rat ovary ; 125I-HCG binding ; Autoradiography ; Hormone receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neither homogenates nor frozen ovarian sections of rats aged 1, 5 and 7 days demonstrated specific binding of 125I-HCG. However, from day 10 to day 35, the homogenates could bind specifically, with an equilibrium association constant in the range of 0.9–2.7 × 1010 M−1. The binding capacity increased from 5.8 fmol/mg tissue at day 10 to 15.7 fmol/mg tissue at day 35. As revealed by autoradiography, the frozen ovarian sections from 10-day-old rats showed 125I-HCG binding localized over the interstitial and thecal tissues and, in the older age groups, also in the granulosa cells of follicles larger than 500 μm in diameter. These results indicate that LH/HCG receptors appear in the rat ovary at the beginning of the second postnatal week, and that the interstitial cells are the main site of action of LH before puberty.
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    Localization of 3H-dihydrotestosterone in the pituitary gland of the rhesus monkey (1981)
    Springer
    Cell & tissue research 215 (1981), S. 499-504 
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    ISSN: 1432-0878
    Keywords: Androgen receptors ; Pituitary gland ; Rhesus monkey ; Autoradiography ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake and retention of radiolabelled dihydrotestosterone by the pituitary gland was examined in the rhesus monkey. Two animals were given an intravenous injection of 1.0μg/kg 3H-dihydrotestosterone (DHT) alone while one monkey received both the labelled androgen and 100μg/kg of unlabelled steroid. One and a half hours later, they were sacrificed. The pituitary glands were removed and processed for autoradiography and immunocytochemistry. Autoradiographic localization of DHT was discernible in the partes nervosa, intermedia and distalis, albeit the highest concentration of radiolabelled cells was noted in the pars distalis. Immunocytochemical staining with antibodies to rat PRL, human TSHβ and ovine LHβ revealed a population of steroid-concentrating cells that contained TSH and a second group that contained LH. None of the cells that reacted with the anti-PRL serum were radiolabelled.
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    Evaluation of the effects of sensory denervation on osteoblasts by 3H-proline autoradiography (1981)
    Springer
    Cell & tissue research 217 (1981), S. 569-576 
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    ISSN: 1432-0878
    Keywords: Autoradiography ; 3H-proline ; Osteoblasts ; Denervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The inferior alveolar nerve was unilaterally resected in 30-day-old mice; other animals were unilaterally sham-operated. At 15, 30, 60, 90, or 150 days after surgery, the mice were injected with 2μCi of 3H-proline (sp. act. 1.0 Ci/mM) per g of body weight and killed 15, 30, or 60 min later. Autoradiographs were prepared from 5μm decalcified sagittal sections of mandibles and grain counts made over periosteal osteoblasts mesial to the first molar. In denervated mandibles, osteoblasts incorporated less isotope compared to controls with differences being maximal at the early intervals. These differences became attenuated with time, possibly due to an intrinsic compensatory mechanism, secondary to neurotrophic regulation.
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    Germinal centers and the B-cell system (1981)
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    Cell & tissue research 218 (1981), S. 59-73 
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    ISSN: 1432-0878
    Keywords: Germinal centers ; B-cell differentiation ; Lymphocyte migration ; Autoradiography ; Lymphoid tissue ; Appendix (rabbit)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The migration pattern of germinal center cells of the rabbit appendix was studied and compared with that of appendix dome cells, spleen cells, thymus cells and thoracic duct lymphocytes. To discriminate T-and B-cell migration pathways, normal or T-cell-depleted rabbits were used as donors. Cell suspensions were labeled in vitro with 3H-leucine followed by intravenous transfer. The migration of labeled cells in lymphoid organs was studied using autoradiography, particular attention being paid to the spleen of the recipient. B-cells from the appendix dome, spleen and thoracic-duct lymph migrate to primary follicles or the corona of secondary follicles via thymus-dependent areas of peripheral lymphoid organs. In contrast, a B-cell subpopulation from the germinal centers of the appendix migrates to the center of splenic primary follicles and into germinal centers. The migration of germinal center cells to splenic follicle centers is not enhanced by specific antigens. The migration properties of B-cells, possibly changing during differentiation, may be instrumental in the two types of immune reactions, i.e., plasma-cell reaction and germinal-center reaction.
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    Localization of 3H-estradiol in the uterus of pregnant and non-pregnant armadillos (1981)
    Springer
    Cell & tissue research 220 (1981), S. 773-780 
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    ISSN: 1432-0878
    Keywords: Estrogen receptors ; Uterus ; Armadillo ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake and retention of radiolabeled estradiol by the uterus was examined in the armadillo. One pregnant and two non-pregnant armadillos were treated with 1.4 μg/kg body weight of 3H-estradiol (E2) by injection into the left ventricle, and one non-pregnant animal was injected with both the labeled hormone and 140 μg/kg body weight of unlabeled E2. One and a half hour after injection, the animals were sacrificed and the uteri were removed and processed for autoradiography. In the non-pregnant animals, nuclear localization was observed in the interstitial cells and glandular epithelium of the endometrium and the connective tissue cells and smooth muscle of the myometrium. Additionally, there was a gradation of uptake in the epithelial cells of the endometrium in that the glandular cells of the basal region were heavily labeled, while those cells in the sinusoidal, and luminal regions contained successively less label. The luminal cells were poorly labeled. In the pregnant female, the smooth muscle and glandular cells hypertrophied and their nuclei contained less label than was observed in the non-pregnant animals. The arteries of the myometrium were more easily distinguished in the pregnant animals and the nuclei of the endothelial cells and smooth muscle were more consistently labeled than those of the non-pregnant armadillos.
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    Autoradiographic localization of 3H-GABA uptake in the thyroid gland of the rat (1981)
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    Cell & tissue research 220 (1981), S. 873-879 
    Details
    ISSN: 1432-0878
    Keywords: γ-Aminobutyric acid (GABA) ; Autoradiography ; Thyroid gland ; Transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The uptake of γ-aminobutyric acid (GABA) in the thyroid gland of the rat was studied autoradiographically following in vitro incubation. High-affinity GABA uptake was localized in follicle cells, whereas C cells (parafollicular cells) in general did not accumulate GABA by high-affinity transport. The follicle cells were also the main sites of low-affinity GABA uptake. Additionally, some nerve fibres were found to accumulate GABA. The predominant localization of GABA uptake in follicle cells is discussed in view of a presumed role of GABA in thyroid function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210468
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  • 45
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    Autoradiographic studies with 3H 1,25 (OH)2 vitamin D3 and 3H 25 (OH) vitamin D3 in rat parathyroid glands (1981)
    Springer
    Cell & tissue research 221 (1981), S. 333-338 
    Details
    ISSN: 1432-0878
    Keywords: 1,25(OH)2 ; Vitamin D3 ; Receptors ; Parathyroid glands ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After injection of radiolabeled 1,25 (OH)2 vitamin D3, nuclear concentration of radioactivity is observed in parenchymal cells of the parathyroid gland in pregnant, adult male, and 10-day male neonatal rats. In competition studies with unlabeled 1,25 (OH)2 vitamin D3, but not with 25 (OH) vitamin D3, nuclear uptake is prevented. Experiments with 3H 25 (OH) vitamin D3, in contrast to 3H 1,25 (OH)2 vitamin D3, do not show nuclear concentration in cells of the parathyroid. The results of the autoradiographic studies suggest the presence of receptors for a direct effect of 1,25 (OH)2 vitamin D3 on the parathyroid gland for modulation of parathyroid hormone secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216737
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  • 46
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    Functions of digestive diverticula in marine invertebrates (1981)
    Springer
    Cell & tissue research 219 (1981), S. 185-195 
    Details
    ISSN: 1432-0878
    Keywords: Glucose ; Pyloric gland ; Ascidian ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The movement of labelled glucose along the digestive tract of two ascidian species, Sidnyum argus and Dendrodoa grossularia is compared. In Dendrodoa grossularia, glucose absorption takes place in all parts of the digestive tract except the oesophagus. Labelled glucose passes through the digestive tract in less than 12 h, and it accumulates in the pyloric ampullae in less than 3 days. In this species, the pyloric gland has more capacity to stock glucidic compounds than other tissues. Under normal feeding conditions, after five days, there is no transfer of labelled glucose to other tissues. Whatever the substances given to Sidnyum argus, its pyloric gland never accumulates labelled products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210027
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  • 47
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    Aspects of cellular proliferation during follicular atresia in the dog ovary (1981)
    Springer
    Cell & tissue research 219 (1981), S. 173-183 
    Details
    ISSN: 1432-0878
    Keywords: Ovary (dog) ; Atresia ; Thymidine ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Autoradiography after pulse labelling with [3H] thymidine was applied to investigate the proliferation processes in the granulosa and theca related to follicular atresia of the dog ovary during metestrus. The number of proliferating cells depends on the follicle type and its atretic stage. There is less proliferation in smaller secondary follicles than either in larger ones or tertiary follicles. While in early atresia tertiary follicles show the highest labelling indices, in advanced atresia the larger secondary follicles are those with the highest values. For each follicle type a decline in the labelling indices can be observed from early to terminal atresia. Tertiary follicles show a precipitous decrease in the labelling index between early and advanced atresia. There is a continuous gradient of proliferation from the center of the follicle over the peripheral granulosa to the theca. In tertiary follicles, an inverse correlation between labelling and necrosis of granulosa cells can be observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210026
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  • 48
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    Electronic Resource
    p-Chlorophenylalanine-induced proliferation of the seminal vesicle epithelium (1981)
    Springer
    Cell & tissue research 219 (1981), S. 159-172 
    Details
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Proliferation ; Autoradiography ; Biochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intraperitoneal injection of p-chlorophenylalanine (pCPA) methylester (100 mg/kg body weight) results in an activation of the lysosomal system of the secretory cells in the rat seminal vesicle and an elevation of the activities of lysosomal enzymes within 15 min following the injection. Large autophagic vacuoles are formed, sequestering rough endoplasmic reticulum and part of the Golgi apparatus within 2 h. Shortly after the activation of the lysosomal system an elevation of both DNA and protein synthesis is measured biochemically. 6 h subsequent to the injection a wave of mitoses of the secretory cells begins, reaching a maximum 6 h later and then declining within 3 h. About 12 h following the injection a second rise in lysosomal activity begins, declining within 24 h. The entire sequence of lysosomal and proliferative activities is inhibited in antiandrogen-pretreated rats. Deduced from these findings the following hypothesis of growth regulation of the accessory sex glands is advanced: enhanced loss of intracellular material during autophagocytosis diminishes the intracellular concentration of a substance curtailing cell division below its effective threshold resulting in division of the secretory cells. The prerequisites of this mechanism are (i) a sufficient distributive capacity of the stroma for hormones (androgens) and metabolic precursors, and (ii) sufficient capacity of the basal cells for transporting the precursors to the secretory cells. Sloughing of the secretory cells separates them from these auxiliary structures (stroma and basal cells) and enables the basal cells to divide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210025
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