Publication Date:
2000-04-25
Description:
Major histocompatibility complex class II (MHC II) molecules capture peptides within the endocytic pathway to generate T cell receptor (TCR) ligands. Immature dendritic cells (DCs) sequester intact antigens in lysosomes, processing and converting antigens into peptide-MHC II complexes upon induction of DC maturation. The complexes then accumulate in distinctive, nonlysosomal MHC II+ vesicles that appear to migrate to the cell surface. Although the vesicles exclude soluble lysosomal contents and antigen-processing machinery, many contain MHC I and B7 costimulatory molecules. After arrival at the cell surface, the MHC and costimulatory molecules remain clustered. Thus, transport of peptide-MHC II complexes by DCs not only accomplishes transfer from late endocytic compartments to the plasma membrane, but does so in a manner that selectively concentrates TCR ligands and costimulatory molecules for T cell contact.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Turley, S J -- Inaba, K -- Garrett, W S -- Ebersold, M -- Unternaehrer, J -- Steinman, R M -- Mellman, I -- AI-13013/AI/NIAID NIH HHS/ -- AI-34098/AI/NIAID NIH HHS/ -- AI-39672/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2000 Apr 21;288(5465):522-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology and Section of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10775112" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal
;
*Antigen Presentation
;
Antigens, CD/immunology/metabolism
;
Antigens, CD86
;
B-Lymphocytes/immunology/metabolism
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Bicyclo Compounds, Heterocyclic/pharmacology
;
Biological Transport
;
Cell Membrane/immunology/metabolism
;
Cells, Cultured
;
Dendritic Cells/*immunology/*metabolism
;
Endocytosis
;
Endosomes/immunology/metabolism
;
Histocompatibility Antigens Class I/immunology/metabolism
;
Histocompatibility Antigens Class II/immunology/*metabolism
;
Kinetics
;
Ligands
;
Lipopolysaccharides/immunology
;
Lysosomes/immunology/metabolism
;
Membrane Glycoproteins/immunology/metabolism
;
Mice
;
Mice, Inbred C3H
;
Muramidase/immunology/*metabolism
;
Peptide Fragments/immunology/*metabolism
;
Receptors, Antigen, T-Cell/metabolism
;
Thiazoles/pharmacology
;
Thiazolidines
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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