Publication Date:
2000-05-08
Description:
The c-Jun NH2-terminal kinase (JNK) is activated when cells are exposed to ultraviolet (UV) radiation. However, the functional consequence of JNK activation in UV-irradiated cells has not been established. It is shown here that JNK is required for UV-induced apoptosis in primary murine embryonic fibroblasts. Fibroblasts with simultaneous targeted disruptions of all the functional Jnk genes were protected against UV-stimulated apoptosis. The absence of JNK caused a defect in the mitochondrial death signaling pathway, including the failure to release cytochrome c. These data indicate that mitochondria are influenced by proapoptotic signal transduction through the JNK pathway.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tournier, C -- Hess, P -- Yang, D D -- Xu, J -- Turner, T K -- Nimnual, A -- Bar-Sagi, D -- Jones, S N -- Flavell, R A -- Davis, R J -- New York, N.Y. -- Science. 2000 May 5;288(5467):870-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Program in Molecular Medicine, Department of Biochemistry & Molecular Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10797012" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Apoptosis
;
Apoptotic Protease-Activating Factor 1
;
Caspase 3
;
Caspase 9
;
Caspases/metabolism
;
Cell Count
;
Cell Division
;
Cells, Cultured
;
Cytochrome c Group/*metabolism
;
DNA Fragmentation
;
Enzyme Activation
;
Fibroblasts
;
Gene Targeting
;
JNK Mitogen-Activated Protein Kinases
;
MAP Kinase Signaling System
;
Methyl Methanesulfonate/pharmacology
;
Mice
;
Mitochondria/metabolism
;
Mitogen-Activated Protein Kinases/genetics/*metabolism
;
NF-kappa B/metabolism
;
*Protein-Serine-Threonine Kinases
;
Proteins/metabolism
;
Proto-Oncogene Proteins/metabolism
;
Proto-Oncogene Proteins c-akt
;
Proto-Oncogene Proteins c-bcl-2/metabolism
;
Tumor Suppressor Protein p53/metabolism
;
Ultraviolet Rays
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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