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  • Springer  (147,273)
  • American Association for the Advancement of Science (AAAS)  (6,940)
  • American Meteorological Society
  • 1995-1999
  • 1990-1994  (64,943)
  • 1980-1984  (92,020)
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  • 1980  (45,098)
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  • 1
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    Bulletin of mathematical biology 42 (1980), S. 131-135 
    ISSN: 1522-9602
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    Topics: Biology , Mathematics
    Notes: Abstract The theory of complementary variational principles is used to obtain maximum and minimum principles for diffusion problems with Michaelis-Menten kinetics. In an illustrative calculation we obtain an extremely accurate variational solution in good agreement with the numerical solution of McElwain (1978).
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  • 2
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    Bulletin of mathematical biology 42 (1980), S. 137-141 
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  • 3
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    Bulletin of mathematical biology 42 (1980), S. 181-189 
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    Notes: Abstract Necessary and sufficient conditions for primitivity of a product of two Leslie matrices are given. Such a product could be used in modeling the growth of a population governed alternately by two different sets of fertility and survival parameters.
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    Bulletin of mathematical biology 42 (1980), S. 173-180 
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    Notes: Abstract Zadeh's transfer function method for linear time-variable systems is used to apply frequency-domain analysis to a periodically time-varying elastance model of the left ventricle. Left ventricular pressure computed from the system function of the time-varying elastance and the phasors of aortic flow shows a typical waveform of the measured ventricular pressure.
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    Bulletin of mathematical biology 42 (1980), S. 901-901 
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    Bulletin of mathematical biology 43 (1981), S. 1-19 
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    Notes: Abstract By studying the behavior of various tracer species in the lungs, one can assess many important characteristics which distinguish normal and abnormal function. Quantitative evaluation of function depends on the use of an appropriate model in conjunction with experimental data. A multi-compartment model is derived from mass balances to describe dynamic as well as (breath-averaged) steady-state transport processes between the environment and pulmonary capillary blood. The breathing cycle is divided into three time periods (inspiration, expiration, and pause) so that the model equations are discrete in time. No other model of tracer species transport in the lungs deals simultaneously with species dynamics, variable breathing pattern, distribution inhomogeneities, and non-equilibrium between alveolar gas and capillary blood. Models currently in the literature are shown to be special cases of the model presented here.
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  • 7
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    Bulletin of mathematical biology 43 (1981), S. 47-58 
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    Notes: Abstract Local stability seems to imply global stability for population models. To investigate this claim, we formally define apopulation model. This definition seems to include the one-dimensional discrete models now in use. We derive a necessary and sufficient condition for the global stability of our defined class of models. We derive an easily testable sufficient condition for local stability to imply global stability. We also show that if a discrete model is majorized by one of these stable population models, then the discrete model is globally stable. We demonstrate the utility of these theorems by using them to prove that the regions of local and global stability coincide for six models from the literature. We close by arguing that these theorems give a method for demonstrating global stability that is simpler and easier to apply than the usual method of Liapunov functions.
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    Bulletin of mathematical biology 43 (1981), S. 125-140 
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    Notes: Abstract The asymptotic behaviour of a logistic equation with diffusion on a bounded region and a diffusionally coupled delay is investigated. An equivelent parabolic system is derived for certain types of delays. Using a Layapunov functional, sufficient conditions for the global asymptotic stability of the constant steady state are obtained. When the global stability is lost, using Hopf's bifurcation theory, existence of travelling waves is shown for ring-like and periodic one dimensional habitats.
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  • 9
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    Bulletin of mathematical biology 43 (1981), S. 141-149 
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    Notes: Abstract It was hypothesized in an earlier work that sensory perception can occur only when the perceiving system is uncertain about the nature of the event being perceived. In the absence of any uncertainty, perception will not take place. The response of the sensory afferent neuron (impulse transmission rate) was calculated using Shannon's measure of uncertainty or entropy. It will now be shown that when the event being perceived is the position and momentum of a particle, Shannon's measure of uncertainty leads to the Heisenberg Uncertainty relationship.
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    Bulletin of mathematical biology 43 (1981), S. 239-244 
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    Notes: Abstract It is not unusual for several classifications to be given for the same collection of objects. We present a method, called majority rule, which can be used to define a consensus of these classifications. We also discuss some mathematical properties of this consensus tree.
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    Bulletin of mathematical biology 43 (1981), S. 259-270 
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    Notes: Abstract The dependence of the spatial concentration profiles of morphogens on a characteristic dimension is obtained by continuation techniques for Gierer and Meinhardt's activator-inhibitor model of morphogenesis. The study of the behaviour of the system during growth, where the linear and exponential increase of the characteristic dimension is considered, revealed that more complex patterns of morphogen spatial concentrations appear regularly in a reproducible way.
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    Bulletin of mathematical biology 43 (1981), S. 271-278 
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    Notes: Abstract Computer models have been used by various authors to simulate both the growth of normal cellular tissue and the development of cancerous cells within normal tissue. As these models were the result of considerable idealization, the purpose of the present paper is to propose a model in which the degree of simplification is relaxed: the features of simultaneous growth, and cell growth whose rate depends on the free absorbing periphery of the cell are introduced. Simulation experiments have been conducted using the model, and the results are presented.
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    Bulletin of mathematical biology 43 (1981), S. 341-346 
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    Notes: Abstract The theory of complementary variational principles is used to obtain maximum and minimum principles for a nonlinear model of heat conduction in the human head. Accurate variational solutions are obtained in illustrative calculations. The effect of nonlinearity is seen to be significant from a comparison with the linearized model.
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    Bulletin of mathematical biology 43 (1981), S. 279-325 
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    Notes: Abstract A model for the nerve impulse due to Zeeman (1972) and based on catastrophe theory is compared with alternative models and criticisms of Zeeman's model by Sussmann and Zahler (1977, 1978) are assessed. The criticisms of Zeeman's motivation for his model are found to carry some weight. Sussmann and Zahler (1977, 1978) list numerous features of Zeeman's model which, they state, are not in agreement with experiment. These statements as they stand are largely erroneous, and the model still remains to be tested by a critical series of experiments. However, a detailed analysis reveals defects in Zeeman's model, not among those claimed by Sussmann and Zahler, showing that the explicit equations of the model cannot be correct. The possibility of a modified approach along similar lines and its ultimate adoption remains open.
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    Bulletin of mathematical biology 43 (1981), S. 375-388 
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    Notes: Abstract The irreversible Michaelis-Menten reaction is studied by the use of the method of multiple scales. Three stages of the reaction are identified, one of which is studied in detail. The results are compared with those of two earlier analyses.
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    Bulletin of mathematical biology 43 (1981), S. 389-400 
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    Notes: Abstract A numerical study of the coupled nerve fibre problem is given which verifies and extends the perturbation theory of Luzader. Pulses on adjacent fibres can couple together with two possible stable pulse separations.
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    Bulletin of mathematical biology 43 (1981), S. 401-413 
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    Notes: Abstract A possible mechanism for effects of microwave radiation on the auditory system is the generation of field-induced forces at interfaces that divide materials of dissimilar electrical properties. A general expression for these “Maxwell stresses” is derived and then used to calculate the approximate magnitude of field-induced force within the organ of Corti during microwave exposure. Comparison of the results with data on the force needed to excite cochlear hair cells indicates auditory responses could be evoked by this mechanism at power densities near the threshold of rf hearing sensations.
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    Bulletin of mathematical biology 43 (1981), S. 415-426 
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    Notes: Abstract A definition of homogeneity for neural networks is given which permits their construction as group quotients. The significance of this for neural dynamics is discussed.
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    Bulletin of mathematical biology 43 (1981), S. 447-461 
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    Notes: Abstract The left ventricle is represented as a cylinder contracting both radially and longitudinally. A simple method is indicated to derive an expression for the rate of change of the kinetic energy of this three-dimensional model, which quantity can be used as an index for the study of the contractile behaviour of the myocardium. An application to the study of muscle mechanics is also indicated.
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    Bulletin of mathematical biology 43 (1981), S. 463-485 
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    Notes: Abstract A perturbation method is proposed to calculate approximately the limit cycle type nonequilibrium steady-state resulting from periodic perturbation of coefficients of stable population systems; the two species Lotka-Volterra competition system is explicity studied and the results are formulated for general multi-species population systems. Avoidance of competitive or other types of exclusion of species in a periodic environment is indicated.
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    Bulletin of mathematical biology 43 (1981), S. 513-516 
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    Bulletin of mathematical biology 53 (1991), S. 281-312 
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    Bulletin of mathematical biology 53 (1991), S. 253-279 
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    Bulletin of mathematical biology 53 (1991), S. 313-326 
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    Bulletin of mathematical biology 53 (1991), S. I 
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    Bulletin of mathematical biology 53 (1991), S. 345-353 
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    Notes: Abstract The statistical correlation of nucleotides in a DNA sequence is described by a set of redundanciesD 1,D 2,D 3,... By calculation of {D n} of 2341 coding regions of nucleic acid sequences it is demonstrated that about 2/3 of sequences has correlation length ≤2, 10% of sequences—correlation with 3-periodicity and others—long range aperiodic correlations. The implications of the results from the interactions of random mutation and natural selection are discussed briefly.
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    Bulletin of mathematical biology 53 (1991), S. 327-343 
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    Notes: Abstract A basic characteristic of biological memory is that it has a graded duration, which, even for socalled short-term memory, can vary from minutes to days (i.e. over about three orders of magnitude), depending on the training protocol, which one can think of as determining the “strength” of the memory. Furthermore, the molecular analysis of simple learning in invertebrates has revealed many examples where “learning” is produced by adecrease in an appropriate membrane conductance. This paper provides a quantitative analysis of a simple kinetic scheme where by a conductance decrease can be produced by repetitive nerve impulses, with a duration that varies with stimulus frequency. The simplest model considered is based on the actual kinetics of the naturally-occurring ionophore Monazomycin. This model yields durations ranging only over a factor of about 10, for reasonable parameter values. However, a simple modification of the model yields memory durations ranging over three or more orders of magnitude. We also show that Monazomycin-like kinetics can appear as the result of a combination of simple uni- and bi-molecular reactions, thus making more plausible the possibility that the effects described here may operate in actual biological systems.
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    Bulletin of mathematical biology 53 (1991), S. 579-589 
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    Notes: Abstract A mathematical model (Kliemann, W. 1987.Bull. math. Biol. 49, 135–152.) that predicts the quantitative branching pattern of dendritic tree was evaluated using the apical and basal dendrites of rat hippocampal neurons. The Wald statistics for χ2-test was developed for the branching pattern of dendritic trees and for the distribution of the maximal order of the tree. Using this statistic, we obtained a reasonable, but not excellent, fit of the mathematical model for the dendritic data. The model's predictability of branching patterns was greatly enhanced by replacing one of the assumptions used for the original model “splitting of branches for all dendritic orders is stochastically independent”, with a new assumption “branches are more likely to split in areas where there is already a high density of branches”. The modified model delivered an excellent fit for basal dendrites and for the apical dendrites of hippocampal neurons from young rats (30–34 days postpartum). This indicates that for these cells the development of dendritic patterns is the result of a purely random and a systematic component, where the latter one depends on the density of dendritic branches in the brain area considered. For apical dendrites there is a trend towards decreasing pattern predictability with increasing age. This appears to reflect the late arrival of afferents and subsequent synaptogenesis proximal on the apical dendritic tree of hippocampal neurons.
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    Notes: Abstract In order for immune cells to carry out many of their functions, including clearance of infectious agents from tissue, they must first encounter their targets in the tissue. This encounter process is often the rate-limiting step in the overall function. Most immune cells exhibit chemotactic ability, and previous continuum models for encounter rates and dynamics have shown that chemotaxis can be a great advantage to cells by greatly increasing encounter rates relative to those for randomly moving cells. This paper describes computer simulations of discrete cell-target encounter events in two dimensions, for the two cases considered by the continuum models: where only a single cell and a single target are present, and where many cells and targets are present. The results of these simulations verify our previous model predictions that a small amount of chemotactic bias dramatically decreases the encounter time, while further increases in the amount of bias have a much smaller effect. Chemotactic ability is shown to be an important determinant of the kinetics of target clearance, and its effects depend on the initial cell-target ratio and the initial distributions of cells and targets. To the best of our knowledge, this work provides the first computer simulations of particle-target encounter in which there is biased motion of particles toward their targets, and is therefore of general interest beyond specific application to immune cell function.
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    Bulletin of mathematical biology 53 (1991), S. 657-663 
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    Bulletin of mathematical biology 53 (1991), S. 881-899 
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    Notes: Abstract The concept of a one-way block, arising from a region of depressed tissue, has remained central to theories for cardiac arrhythmias. We show that both the geometry of a depressed region and spatial heterogeneities in depression are key factors for inducing such a block. By using an asymptotic approximation, known as the eikonal equation, to model qualitatively the movement of a depolarization wave-front down a Purkinje fibre bundle, we show how a one-way block in conduction may result from asymmetric constriction in the width of a depressed bundle. We demonstrate that this theory is valid for biologically relevant parameters and simulate a one-way block by numerically solving the eikonal approximation. We consider the case of non-uniform depression, where the planar travelling wave speed is spatially dependent. Here, numerical simulations indicate that such a spatial dependency may, in itself, be sufficient to produce a one-way block.
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    Bulletin of mathematical biology 53 (1991), S. 911-940 
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    Notes: Abstract The MacArthur-Wilson equilibrium theory of island biogeography has been one of the more influential concepts in modern biogeography and ecology. In this paper, we synthesize the theory and examine effects of different immigration/extinction rate-species diversity curves on original predictions from the theory by using the System Dynamics simulation modeling approach. Moreover, we develop a comprehensive and generic System Dynamics model to incorporate a variety of recent modifications and extensions of the theory, including area effect, distance effect, competition effect, habitat diversity effect, target effect, and rescue effect. Through computer simulation with STELLA, a more profound understanding of the theory of island biogeography can be gained. The System Dynamics modeling approach is especially appropriate for such a study because it maximizes the utilization of the ecological data by incorporating qualitative information so that a complex, imprecisely-defined ecological system can be studied quantitatively, effectively, and comprehensively. Our simulation results show that different monotonic rate-species diversity curves do not affect the essence of the theory of island biogeography, while the magnitude of equilibrium species diversity may be greatly affected. Non-monotonic rate-species diversity curves may result in potential multiple equilibria of species diversity. In addition, our model suggests that a non-monotonic relationship may exist between the equilibrium turnover rate and island area and between the equilibrium turnover rate and distance.
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    Bulletin of mathematical biology 53 (1991), S. 665-678 
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    Notes: Abstract A method to estimate a lower bound of the Kolmogorov entropy—the so calledK 2-entropy—from a time series is presented which avoids use of the generalized correlation integral. The influence of the norm is studied. The method is demonstrated on some standard examples. The entropy of the attractor apparent in the EEG of the foetal sheep is estimated and the results are compared with results obtained from synthesized data featuring some basic properties of EEG. This gives an insight into the limitations of the procedure.
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    Bulletin of mathematical biology 53 (1991), S. 685-700 
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    Notes: Abstract The autonomous oscillations in yeast continuous cultures are investigated analytically and related to the behaviour of the single cell by means of a suitable modified version of Monod’s classical chemostat model. Two main cell phases or states are considered to account for the experimentally observed changes occurring in the cell growth course: the budded phase and the unbudded one. Thus, a sort of two compartment structure is given to the total biomass. The model so far obtained allows one to analyse the local properties of the predicted steady states under various assumptions, both on the yield coefficients and the specific growth rates. Necessary conditions for the local instability are derived and the existence of stable limit cycles is shown by computer simulation. With respect to the qualitative changes in the metabolic parameters, this analysis agrees with the results obtained by simulation of complex structured and segregated models. However, the oscillation period is too long compared with the experimental one and this fact may be mainly due to the strong simplifying assumptions on the dynamic evolution of the transfer rates between the two compartments. The model’s usefulness seems until now restricted to the identification of the relationships between the cell cycle regulation and the oscillation triggering.
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    Notes: Abstract The mathematical model developed by Riveroet al. (1989,Chem. Engng Sci. 44, 2881–2897) is applied to literature data measuring chemotactic bacterial population distributions in response to steep as well as shallow attractant gradients. This model is based on a fundamental picture of the sensing and response mechanisms of individual bacterial cells, and thus relates individual cell properties such as swimming speed and tumbling frequency to population parameters such as the random motility coefficient and the chemotactic sensitivity coefficient. Numerical solution of the model equations generates predicted bacterial density and attractant concentration profiles for any given experimental assay. We have previously validated the mathematical model from experimental work involving a step-change in the attractant gradient (Fordet al., 1991Biotechnol. Bioengng.37, 647–660; For and Lauffenburger, 1991,Biotechnol. Bioengng,37, 661–672). Within the context of this experimental assay, effects of attractant diffusion and consumption, random motility, and chemotactic sensitivity on the shape of the profiles are explored to enhance our understanding of this complex phenomenon. We have applied this model to various other types of gradients with successful intepretation of data reported by Dalquistet al. (1972,Nature New Biol. 236, 120–123) forSalmonella typhimurum validating the mathematical model and supportin the involvement of high and low affinity receptors for serine chemotaxis by these cells.
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    Bulletin of mathematical biology 53 (1991), S. 797-804 
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    Bulletin of mathematical biology 53 (1991), S. 845-851 
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    Notes: Abstract Disconnected recurrences of the stop signal, serine and arginine appear in the original representation of the genetic code, and of the stop signal, arginine, serine and leucine in the codon ring representation. To achieve connectedness along with structural continuity, arook’s tour representation is presented here. On the basis of structural similarities and disparities in their side groups, each of the 20 amino acids is associated with a domain comprised of from one to six contiguous squares on the chess board. As the rook moves on the chess board, it reaches all 64 squares in the ordering of the codon numbers, which prescribe the codons by a simple formula based on the position and size of the nucleotides in a triplet. Recurrences of the stop signal, arginine and serine occur naturally on the tour as the rook enters each of the latter domains for the second time. A mathematical equivalent of the rook’s tour may enter as a programming device in the implementation of the code by the RNAs.
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    Circuits, systems and signal processing 10 (1991), S. 71-89 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract We consider general input-output systems, which need not be of a feedback type, that are governed by nonlinear operator equations which relate the input, state, and output. Assuming that these equations depend on a parameterA which is allowed to vary in a neighborhood of a “nominal value”A 0 , we study the dependence of the output onA when the input is fixed. Essentially, we call a system insensitive if the output depends continuously onA. Two insensitivity concepts are introduced, and it is shown that certain monotonicity-like conditions ensure insensitivity. Also, several particular cases of the governing equations are studied. As examples, a control system described by a singular system of ordinary differential equations and a nonlinear feedback system are discussed.
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    Circuits, systems and signal processing 10 (1991), S. 153-161 
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The relationship between the elements in the vector of any limit cycle due to rounding in ann-order direct-form digital filter is established. Some bounds on the elements in such vectors are also determined. Sufficient conditions for the accessibility of period-r limit cycles due to rounding inn-order digital filters are presented.
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    Circuits, systems and signal processing 10 (1991), S. 327-342 
    ISSN: 1531-5878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract This paper analyzes two direction-of-arrival (DOA) estimation algorithms used in the presence of multipath propagation and with very few snapshots. The conditional maximum likelihood (CML) algorithm and the method of direction estimation (MODE) are discussed. The estimates provided by these algorithms are shown to coincide for large number of snapshots or large signal-to-noise ratio. Necessary and sufficient conditions are derived for the algorithms to yield unique estimates. It is shown that their uniqueness conditions coincide with the minimal uniqueness condition on the array, that is independent of the algorithm used (if the array does not satisfy this minimal condition, no DOA estimation method can give unique estimates). Numerical examples are presented to demonstrate the theoretical results.
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    Circuits, systems and signal processing 10 (1991), S. 361-389 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract In Part I of this paper we consider a general model of an input-output system governed by nonlinear operator equations that relate the system's input, state, and output, all of which are elements in extended spaces. This model encompasses feedback systems as a special case. Assuming that the equations governing the system depend on a parameterA that is allowed to vary in a neighborhoodN r (A 0) of a nominal valueA 0 in a linear space, we study conditions under which the system is stable for everyA ε Nr(A0), i.e., when the system exhibits robust stability. By stability we essentially mean that the input-output operator is continuous. Depending on the type of continuity of a map between two extended spaces, four concepts of robustness are introduced. The main results, Theorems 1 and 2, furnish sufficient conditions for a system to be robust in the respective sense. Basically, they show that if the nominal system satisfies a certain condition guaranteeing its stability, and the operators appearing in the governing equations depend continuously on the parameterA, then we have robust stability. As examples illustrating the applications of our results we discuss (1) a feedback-feedforward system, in particular the case when the extended space consists of locally square-integrable functions or functions continuous on [0, ∞), and (2) a time-varying dynamical system described by a linear vector differential equation, whose variables are continuous functions on [0, ∞) which decrease exponentially to zero ast → ∞. At the end of the paper some modifications of the presented theory are discussed.
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    Circuits, systems and signal processing 10 (1991), S. 485-511 
    ISSN: 1531-5878
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Higher-than-second-order statistics-based input/output identification algorithms are proposed for linear and nonlinear system identification. The higher-than-second-order cumulant-based linear identification algorithm is shown to be insensitive to contamination of the input data by a general class of noise including additive Gaussian noise of unknown covariance, unlike its second-order counterpart. The nonlinear identification is at least as optimal as any linear identification scheme. Recursive-least-squares-type algorithms are derived for linear/nonlinear adaptive identification. As applications, the problems of adaptive noise cancellation and time-delay estimation are discussed and simulated. Consistency of the adaptive estimator is shown. Simulations are performed and compared with the second-order design.
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    The Geneva risk and insurance review 16 (1991), S. 59-74 
    ISSN: 1554-9658
    Keywords: Unemployment Insurance ; Risk Sharing ; Social Security
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract Two models of spot labor markets are presented in which labor suppliers have heterogeneous attitudes towards effort and in which uncertainty prevails on labor productivity and growth. The problem of selecting efficient rules to manage unemployment insurance (UI) systems is considered. We show that there does not exist any system which combines an efficient allocation of labor with an efficient allocation of risks among employees, unemployed workers and capital-owners. Pareto-efficient policy rules are a best compromise between these two conflicting objectives. It implies that productive efficiency could be improved in periods of mass unemployment by reducing UI benefits. That would be at the expense of more inefficiencies in the sharing of macroeconomic risks. At the optimum, the UI benefit is positively correlated to growth and it is negatively correlated to labor productivity.
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    The Geneva risk and insurance review 16 (1991), S. 137-138 
    ISSN: 1554-9658
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    Topics: Economics
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    The Geneva risk and insurance review 16 (1991), S. 139-141 
    ISSN: 1554-9658
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    Topics: Economics
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    The Geneva risk and insurance review 16 (1991), S. 143-165 
    ISSN: 1554-9658
    Keywords: Social Insurance ; Redistribution ; Incentives
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    Topics: Economics
    Notes: Abstract We extend the familiar income taxation model à la Mirrlees, so as to include income uncertainty, due for instance to a risk of illness. Following a line of research initiated by Blomqvist and Horn [1984] we prove that the existence of a Social Health Insurance system may be justified even when the insurance market is efficient. Moreover, if there is a negative statistical dependence between probability of illness and labor productivity, then the optimum of a Utilitarianist Social Welfare function implies that Social Insurance provides a complete coverage for every household.
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    The Geneva risk and insurance review 16 (1991), S. 167-177 
    ISSN: 1554-9658
    Keywords: Risk Taking ; Taxation ; Complete Conventional Securities Markets
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    Topics: Economics
    Notes: Abstract In general equilibrium, with complete conventional securities markets and endogenous asset supply, taxes on risk remuneration are ineffective but harmless. They do not alter the real allocation of goods or the distribution of wealth, they impose no excess burden, and, in particular, have no impact on risk taking.
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  • 49
    ISSN: 1476-5535
    Keywords: Actinomycete ; Biotransformation ; pH control ; Magnesium sulfate ; MK-733 ; Simvastatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary An actinomycete (MA 6474, ATCC 53828) isolated from a soil sample (Mutare, Zimbabwe) was found to biotransform the sodium salt of Simvastatin (MK-733) to 6-α-hydroxymethyl MK-733, 6-β-hybroxymethyl MK-733, and 6-ring-hydroxy MK-733. The bioconversion efficiency to the desired compound, 6-α-hydroxymethyl MK-733, was enhanced by optimizing the physico-chemical parameters of the process. In shake flask cultures, addition of magnesium (0.125 mg/l Mg SO4·7H2O) to the medium resulted in a five-fold increase in the rate of bioconversion to the α diastereomer. The ratio of bioconversion products (6-α-hydroxymethyl, 6-β-hydroxymethyl, and 6-ring-hydroxy MK-733) was regulated by pH. Process improvements and scale up in 23-1 fermentors, which consisted of a controlled addition of substrate (MK-733), resulted in a 2-fold increase in alpha diastereomer Production (42 vs. 79 U/ml) and a 23-fold rate increase in the formation of α-diastereomer. A high diastereomeric ratio (α: β=9∶1) facilitated downstream processing.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 147-156 
    ISSN: 1476-5535
    Keywords: Methanol ; Yeast extract ; Two-phase process ; Periplasmic antigen ; Intracellular antigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Various physico-chemical parameters have been studied in order to improve the production of hepatitis B virus pre-S2 antigen (middle surface antigen) by the methylotrophic yeastHansenula polymorpha. Antigen production was done in two steps: first, production of cells on glycerol (Phase 1), followed by induction of antigen expression with methanol (Phase 2). Dense cultures ofH. polymorpha, equivalent to 35–40 g/l (dry weight), were readily obtained in small fermenters using minimal medium containing glycerol as carbon source. Antigen expression in this minimal medium, after induction with methanol, was however low and never exceeded 1.6 mg/l of culture. Antigen production was greatly enhanced by adding complex organic nitrogen sources along with methanol at induction time; yeast extract was the best of all the sources tested. In shake flasks, antigen production was proportional to yeast extract concentration up to 7% (w/v) yeast extract. it became clear that the nutritional conditions for good antigen expression were different from those for good biomass production. The effects of yeast extract were reproduced in small fermenters: antigen levels reached 8–9 mg/l in medium containing 6% (w/v) yeast extract during induction with methanol. The mechanisms of yeast extract's effects are still unknown but are probably nutritional. The recombinantH. polymorpha strain produced both periplasmic and intracellular antigen. The periplasmic antigen was shown to be present as 20–22-nm particles and was therefore immunogenic. Immunoblotting indicated that part of the pre-S2 antigen was present as a 24-kDa degradation product. These studies have led to a 140-fold increase in volumetric productivity of antigen and to a 4.6-fold increase in specific production.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 171-178 
    ISSN: 1476-5535
    Keywords: EPA ; Omega-3 ; Arachidonic acid ; Polyunsaturated fatty acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effect of culture conditions upon lipid content and fatty acid composition of mycelia ofPythium irregulare was investigated with particular attention to increasing the yield of 5,8,11,14,17-eicosapentaenoic acid (20∶5; ω−3) (EPA). All experiments were done by shake flask culture using a yeast extract + malt extract medium. The maximum growth rate was obtained at 25°C, but maximum EPA production was obtained at 12°C. The highest EPA production was 76.5 μg EPA/ml 13 days fermentation at 12°C. Addition of glucose during fermentation increased the yield considerably. The highest yield was 112 μg/ml, obtained at 13 days fermentation with spiking on day 11. Fermentation time could be shortened by initial incubation at 25°C for 2 days, followed by incubation at 12°C for 6 days. The culture also produced arachidonic acid and other ω-6 polyunsaturated fatty acids. EPA production was also obtained with lactose or sweet whey permeate, a by-product of cheese manufacture that contains lactose as the main carbohydrate.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 179-185 
    ISSN: 1476-5535
    Keywords: Mortierella alpina ; Arachidonic acid ; Polyunsaturated fatty acid ; Fungal lipid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary WhenMortierella alpina ATCC 32222 was incubated in a glucose salts medium at 25°C the biomass (17.5 g/l) contained 9.62% arachidonic acid which amounted to 54% (w/w) of total biomass lipids. When the glucose concentration in the medium was varied from 0 to 150 g/l, the percentage of arachidonic acid in biomass and in lipids was highest at a glucose concentration of 30 g/l, but highest yield of arachidonic acid per litre of culture broth was observed at a glucose concentration of 100 g/l. While production of biomass reached a plateau of 17 g/l after a 3-day incubation at 25°C, the percentage of arachidonic acid in lipids and biomass increased dramatically from 3 to 6 days with a concurrent arachidonic acid yield increase from 0.89 to 1.63 g/l. Optimum initial culture pH for arachidonic acid production was in the range 6.0–6.7. By increasing the concentration of the glucose salts medium three-fold, yields of biomass and arachidonic acid were increased to 35.8 g/l and 3.73 g/l, respectively.
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  • 53
    ISSN: 1476-5535
    Keywords: Dopamine receptor ; Agonist and antagonist ; Ligand ; Dihydroxy acetanilide
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A natural product, Sch 42029, isolated from the fermentation of anActinoplanes sp. (SCC 1971) was found to displace Sch 23390 from the dopamine-1 (D1) receptor. The compound was isolated from the fermentation broth by adsorption of the filtrate on XAD-16 resin, elution with water-methanol, followed by purification by gel-permeation chromatography and HPLC. Using spectroscopic analysis, the structure was determined to be 2,5-dihydroxy acetanilide. The pure compound displaced Sch 23390, a D1-selective ligand, at aK i of 1.6 μm and spiperone, a D2-selective ligand, at aK i of 200 μm.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 193-199 
    ISSN: 1476-5535
    Keywords: Organic hazardous waste ; Leachate ; Landfill management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Co-disposal of 12 compounds representing major organic classes (aromatic hydrocarbons, halogenated hydrocarbons, pesticides, phenols, and phthalate esters) with shredded municipal solid waste was tested using a laboratory-scale column and pilot-scale lysimeter to characterize transport and transformation phenomena including sorption, volatilization and bioassimilation. Leachate and gases emitted from the lysimeters were examined for identifiable products of biotransformation. The results of this investigation provided a mechanistic evaluation of the attenuating and assimilative capacity of municipal solid waste landfills for specific organic compounds. Physical/chemical organic compound characteristics were related to refuse characteristics and composition to predict compound fate. Such knowledge is useful in developíng landfill management and operational strategies consistent with the need for control of pollutant releases.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 201-207 
    ISSN: 1476-5535
    Keywords: Diffusion chamber ; Cadmium-sensitive ; Cadmium-resitant ; Sediment ; Bacteria ; Cadmium-sorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Sorption of cadmium by sediment bacteria and freshwater sediment was investigated using diffusion chambers to simulate the water-sediment interface. Diffusion chambers were constructed to provide two compartments separated by a dialysis membrane. Diffusion of cadmium across the membrane was monitored after pure cultures of sediment bacteria or lake sediments were added to the sediment side of a diffusion chamber. Cellular accumulation of cadmium by cadmium-sensitive and cadmium-resistant bacteria removed between 20% and 80% of the dissolved cadmium from the simulated water column and pore water. Cellular accumulation of cadmium was greatest for cadmium-sensitive isolates that were tested. Sediment with an intact microbial community sequestered 80% of the cadmium added to sediment, whereas autoclaved sediment retained 97% of the metal that was added. Addition of glucose to cadmium-amended sediment decreased retention of cadmium by untreated and autoclaved sediments, resulting in elevated concentrations of dissolved cadmium in the simulated water column.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 209-212 
    ISSN: 1476-5535
    Keywords: Biodegradation ; Direct method ; Indirect method ; Method comparison ; BOD method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Degradation of 10 organic chemicals by pre-acclimated microorganisms in BOD dilution water was determined directly by UV spectrophotometry and indirectly by a modified BOD method. Residual chemical concentrations were periodically measured and pseudo-first-order biodegradation rate constants (k 1) were calculated. Thek 1 spectrophotometry values ranged from 0.006/h to 0.077/h andk 1-BOD values from 0.002/h to 0.043/h for 1-methylnaphthalene and indole, respectively. The ratios ofk spectrophotometry to k1-BOD were between 1.5 for salicylic acid and 3.0 for 1-methylnaphthalene with a mean of 2.7. A significant (α=0.001) linear correlation (r 2=0.854,F=46.630) existed between the two sets of rate constants. Results from this study suggest that the modified BOD method may be used to estimate chemical biodegradation rates in synthetic media.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 213-221 
    ISSN: 1476-5535
    Keywords: Biofilm ; Scanning electron microscope ; Environmental scanning electron microscope
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Descriptions of biofilms and their elemental compositions based on scanning electron micrographs and energy dispersive x-ray analysis cannot be related to the original condition of the biofilm on the surface. Solvent replacement of water removes extracellular polymeric material and reduces the concentration of elements bound within the biofilm. In the wet state, bacteria and microalgae are enmeshed in a gelatinous film that is either removed or dried to a thin inconspicuous residue during sample preparation for scanning electron microscopy. The environmental scanning electron microscope provides a fast, accurate image of biofilms, their spatial relationship to the substratum and elemental composition.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 223-227 
    ISSN: 1476-5535
    Keywords: Deionized water ; Ultra-pure water ; Ozone ; Ultra-violet sterilization ; Oligotroph ; Bacteria ; R2A medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Presently, tryptic soy agar (TSA) medium is used in the semiconductor industry to determine the concentration of viable oligotrophic bacteria in ultra-pure water systems. Deionized water from an ultra-pure water pilot plant was evaluated for bacterial growth at specific locations, using a non-selective medium (R2A) designed to detect injured heterotrophic as well as oligotrophic bacteria. Results were compared to those obtained using Tryptic Soy Agar. Statistically greater numbers of bacteria were observed when R2A was used as the growth medium. Total viable bacterial numbers were compared both before and after each treatment step of the recirculating loop to determine their effectiveness in removing bacteria. The reduction in bacterial numbers for the reverse osmosis unit, the ion exchange bed, and the ultraviolet sterilizer were 97.4%, 31.3%, and 72.8%, respectively, using TSA medium, and 98.4%, 78.4%, and 35.8% using R2A medium. The number of viable bacteria increased by 60.7% based on TSA medium and 15.7% based on R2A medium after passage of the water through an in-line 0.2-μm pore size nylon filter, probably because of the growth of bacteria on the filter. Our results suggest that R2A medium may give a better representation of the microbial water quality in ultra-pure water systems and therefore a better idea of the effectiveness of the various treatment processes in the control of bacteria.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 229-236 
    ISSN: 1476-5535
    Keywords: Mannanase ; Sporotrichum cellulophilum ; Galactomannan ; Hemicellulase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Extracellular mannanase activity produced bySporotrichum cellulophilum was purified into two components using acetone precipitation, SP-Sephadex C50 ion exchange chromatography and preparative polyacrylamide gel electrophoresis. The purified mannanse components, M1 and M2, had molecular weights of 108 000–112 000 and 32 200–36 000 respectively. Component M1 was shown to contain 2 subunits having molecular weights of 62 000 and 50 000. M1 and M2 had similar pH-activity profiles with pH optima of 5.5 and 6.0 respectively. M1 was more thermostable than M2: half lives of the enzymes at 70°C were 30 and 9 min for M1 and M2 respectively.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 237-245 
    ISSN: 1476-5535
    Keywords: Microbial emulsifier ; Biosurfactant ; Bioemulsifier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Biosurfactants have potential for use in enhancement of in situ biorestoration by increasing the bioavailability of contaminants. Microorganisms isolated from biostimulated, contaminated and uncontaminated zones at the site of an aviation fuel spill and hydrocarbon-degrading microorganisms isolated from sites contaminated with unleaded gasoline were examined for their abilities to emulsify petroleum hydrocarbons. Emulsifying ability was quantified by a method involving agitation and visual inspection. Biostimulated-zone microbes and hydrocarbon-degrading microorganisms were the best emulsifiers as compared to contaminated and uncontaminated zone microbes. Biostimulation (nutrient and oxygen addition) may have been the dominant factor which selected for and encouraged growth of emulsifiers; exposure to hydrocarbon was also important. Biostimulated microorganisms were better emulsifiers of aviation fuel (the contaminant hydrocarbon) than of heavier hydrocarbon to which they were not previously exposed. By measuring surface tension changes of culture broths, 11 out of 41 emulsifiers tested were identified as possible biosurfactant producers and two isolates produced large surface tension reductions indicating the high probability of biosurfactant production.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 247-252 
    ISSN: 1476-5535
    Keywords: Invertase ; Entrapped yeast ; Ethanol pretreatment ; Heat pretreatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Gel-entrapped, non-viable yeast biomass with specific invertase activity has been produced by two different pretreatment protocols: a short-time thermal treatment and a brief contact with concentrated ethanol solutions. Four yeast strains were most promising:K. fragilis L-293,C. utilis L-282,S. cerevisiae L-170 and L-209. Of these, the ethanol-tolerant L-282 and the ethanol-tolerant and heat-resistant L-170 gave the most active gel-entrapped biocatalysts: around 2 mg of reducing sugars produced per mg dry yeast per min.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 253-258 
    ISSN: 1476-5535
    Keywords: Cholesterol ; 4-Cholesten-3-one ; Cholesterol oxidation ; Heterologous gene expression ; Streptococcal vector
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A streptomycete gene coding for extracellular cholesterol oxidase (choA) was subcloned and expressed inEscherichia coli. The pUCO series recombinants were obtained by inserting thechoA gene into the uniqueKpnI site of pUC19 vector. Expression was observed with pUCO192A and pUCO193 constructs in which the cloned gene(s) were aligned with the upstreamlacZ promoter. Isopropyl β-d-thioglucopyranoside (IPTG) enhanced this expression up to 2.5-fold. Specific Cho activity in the cell extracts of the stable pUCO193 transformant were 0.004 U and 0.007 U per mg protein without and with IPTG induction, respectively. Cho activity was detected in the spent medium of this culture, suggesting possible secretion of the enzyme.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 273-276 
    ISSN: 1476-5535
    Keywords: Bacterial resistance ; Isothiazolone, Quarternary ammonium compounds ; Thiocarbamate ; Water cooling system ; Pseudomonas cepacia ; Pseudomonas stutzeri ; Bacillus subtilis ; Bacillus cereus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Bacteria from water cooling systems developed resistance to three different bactericides i.e. quarternary ammonium compound (QAC), isothiazolone and thiocarbamate. Resistance was induced by exposing isolates to increasing sublethal concentrations for a period of 10 weeks.Bacillus subtilis became resistant to 1000 mg l−1 QAC. Cross-resistance was also detected, e.g. isothiazolone induced resistance to QAC and thiocarbamate.
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    Journal of industrial microbiology and biotechnology 8 (1991), S. 265-271 
    ISSN: 1476-5535
    Keywords: Efrotomycin ; Nocardia lactamdurans ; Uracil catabolism
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Nocardialactamdurans has been shown to catabolyse uracil via the reductive pathway. The end product of this pathway, β-alanine, is incorporated into the pyridone ring of efrotomycin. Support for this proposal includes: (1) reversal of thymine inhibition of efrotomycin biosynthesis by dihydrouracil andN-carbamoyl-β-aline, two intermediates of the catabolic pathway; (2) incorporation of [5,6-3H]-uracil into efrotomycin with a relative molar specific activity of approximately 0.5, close to the theoretical maximum; and (3)13C coupling at C4 and C5 of efrotomycin after feeding resting cells with [4,5-13C]-uracil. Our results do not rule out the possibility of an alternative source of β-alanine or the coexistence of uracil catabolism via oxidative reactions.
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    Bulletin of mathematical biology 42 (1980), S. 147-160 
    ISSN: 1522-9602
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    Topics: Biology , Mathematics
    Notes: Abstract A theory of noise fluctuations is developed which is applicable to systems of any size in which unimolecular or bimolecular reactions are occurring. The main difference between small and large reacting systems is that in the former the probability of finding a particle in a particular state does not obey a Gaussian distribution, but satisfies a distribution which reflects the mechanism of the chemical reaction. This difference is reflected in the main result of the theory: an autocorrelation function that is expressible as a sum of exponentials, the amplitudes of which are explicit functions of the moments of the distribution. Thus, by using small systems, the autocorrelation function,in principle, allows the elucidation of reaction mechanisms. Numerical simulations indicate that for reacting systems having ten or fewer particles, the deviation of the autocorrelation function from a single exponential should be easily detectable, and that estimates of the first four moments of the distribution should be possible. Accurate inference of the distribution, however, will require further mathematical and experimental advances.
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    Bulletin of mathematical biology 42 (1980), S. 161-172 
    ISSN: 1522-9602
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    Topics: Biology , Mathematics
    Notes: Abstract The recent mathematical formalization of the concepts of matter and extrinsical energy, which are used for the relational representation of biological systems, is employed in the analysis of the important experimental discoveries of Comorosanet al. related to low energy electromagnetic irradiations on enzyme substrates. By means of the present analysis one of the properties inherent to the experimental phenomena is more precisely exposed, and theoretical developments corresponding to “energetical evolutions” in a biological system (Leguizamón, 1976) may now have an experimental basis. Important limitations are introduced for the validity of the commutativity and associativity of cartesian product of sets, when they represent matter and its linked extrinsical energy. In connection with this last aspect, new important knowledge is obtained for the relational mathematical representation of biological systems.
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    Bulletin of mathematical biology 42 (1980), S. 397-429 
    ISSN: 1522-9602
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    Topics: Biology , Mathematics
    Notes: Abstract The structure of solutions to a simple spatially dependent population model involving growth and death is investigated. Two forms of motility of the population are considered: (1) random motion only modeled by a Fickian law, and (2) a directed component of motion (chemotaxis), included in addition to the random motion. Under certain growth conditions a traveling wave of constant speed is approached. This speed can be increased by the addition of the chemotaxis with a corresponding increase in the asymptotic population. Development of initial conditions into a wave is illustrated numerically.
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    Bulletin of mathematical biology 42 (1980), S. 365-396 
    ISSN: 1522-9602
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    Notes: Abstract This paper describes mechanisms of intracellular and intercellular adaptation that are due to spatial or temporal factors. The spatial mechanisms support self-regulating pattern formation that is capable of directing self-organization in a large class of systems, including examples of directed intercellular growth, transmitter production, and intracellular conductance changes. A balance between intracellular flows and counterflows causes adaptation. This balance can be shifted by environmental inputs. The decrease in Ca2+-modulated outward K+ conductance in certain molluscan nerve cells is a likely example. Examples wherein Ca2+ acts as a second messenger that shunts receptor sensitivity can also be discussed from this perspective. The systems differ in basic ways from recent diffusion models. Chemical transducers driven by membrane-bound intracellular signals can establish long-range intercellular interactions that compensate for variable intercellular distances and are invariant under developmental size changes; diffusional signals do not. The intracellular adaptational mechanisms are formally analogous to intercellular mechanisms that include cellular properties which are omitted in recent reaction-diffusion models of pattern formation. The cellular models use these properties to compute size-invariant properties despite wide variations in their intercellular signals. Mechanisms of temporal adaptation can be derived from the simplest laws of chemical transduction by using a correspondence principle. These mechanisms lead to such properties of intercellular signals as transient overshoot, antagonistic rebound, and an inverted U in sensitivity as intracellular signals or adaptation levels shift. Such effects are implicated in studies of behavioral, reinforcement, motor control, and cognitive coding.
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    Bulletin of mathematical biology 42 (1980), S. 447-459 
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    Notes: Abstract Large radiation doses to the lung can cause early death from cardiopulmonary insufficiency resulting from radiation pneumonitis and pulmonary fibrosis. A model for early death following inhalation of insoluble radioactive particles is propose. The model is based on three assumptions: (1) early death results from damage to a cluster of cells from a large number of cell clusters at risk, (2) the dose that causes early death depends on how the radiation is delivered in time and (3) the cell clusters at risk to damage are equally sensitive ro radiation. Results from asymptotic theory of extreme values, along with biophysical considerations, suggest that the cumultive distribution function for the absorbed radiation dose to the production of pulmonary injury sufficient to cause early death is best estimated by the third asymptotic distribution without a threshold. This distribution function is identical to the Weibull cumulative distribution function. Data for Beagle dogs after inhaling relatively insoluble forms of alpha- or beta-gamma-emitting particles are shown to support the Weibull model.
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    Bulletin of mathematical biology 42 (1980), S. 461-480 
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    Notes: Abstract Models of the human respiratory tract were developed based on detailed morphometric measurements of a silicone rubber cast of the human tracheobronchial airways. Emphasis was placed on the “Typical Path Lung Model” which used one typical pathway to represent a portion of the lung, such as a lobe, or to represent the whole lung. The models contain geometrical parameters, including airway segment diameters, lengths, branching angles and angles of inclination to gravity, which are needed for estimating inhaled particle deposition. Aerosol depositions for various breathing patterns and particle sizes were calculated using these lung models and the modified Findeisen-Landahl computational scheme. The results agree reasonably well with recent experimental data. Regional deposition, including lobar deposition fractions, are also calculated and compared with results based on the ICRP lung deposition model.
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    Bulletin of mathematical biology 42 (1980), S. 481-488 
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    Notes: Abstract The completely symmetrical system is defined as having identical transfer coefficients between pairs of compartments and the same loss coefficient for each compartment. The eigenvalues and eigenvector are explicitly found along with the inverses of the system matrix and the matrix of eigenvectors. Many properties, special instances of more general theorems, can be seen at once from the explicit analytic solution of the initial value, washout and washin problems. The system serves as a known case for testing estimation procedures, algorithms for solutions of linear systems, eigenvalue-eigenvector and inversion routines and is of considerable tutorial value.
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    Bulletin of mathematical biology 42 (1980), S. 431-446 
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    Notes: Abstract The mathematical structures underlying the theories of organismic sets, (M, R)-systems and molecular sets are shown to be transformed naturally within the theory of categories and functors. Their natural transformations allow the comparison of distinct entities, as well as the modelling of dynamics in “organismic” structures.
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    Bulletin of mathematical biology 42 (1980), S. 489-505 
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    Notes: Abstract To explain the sodium conductance change using Wei's dipole model (Wei, 1969), we may expect that during depolarization the dipole's population difference, ΔN, is first reduced and then returns more slowly to its resting value. This paper shows that the experimental results of gating currents support this idea. Such time course of ΔN, however, is not a usual relaxation process. To account for the unusual behavior of ΔN, we propose two additional assumptions: (1) there exists a special coupling system (probably the intramolecular vibrations) whose coupling strength with the dipoles is much stronger than with the thermal bath (intermolecular vibrations), and (2) there also exist “traps” for the dipole's excitation energy so that this energy is transformed into other energy forms at a rate increasing with the increase of depolarization. Experiments suggest that the traps are proteins located at the inner membrane surface.
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    Bulletin of mathematical biology 42 (1980), S. 507-528 
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    Notes: Abstract Current research into the dynamics of iterative ecological and biological models has lead to a number of theorems concerning the existence of various types of iterative dynamical behavior. In particular, much study has been done on the dynamical behavior of the “simplest dynamical system”f b(x)=bx(1−x), which is just the canonical discrete form of logistic growth equations found in ecology, sociobiology, and population biology. In this paper, we make use of some of the techniques and concepts of topological dynamics to construct a number of generalized conjugacy theorems. These theorems are then used to demonstrate that the mappingf b has a number of conjugacy classes in which the dynamics of the iterates is equivalent to within a change of variables. The concepts of fitness and survival in logistic equations are then shown to be independent, if we follow certain intuitive definitions for these concepts. This conclusion follows from a comparison of the conjugacy classes of the functionf b and the extinction sets off b.
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    Notes: Abstract For chemical reactions not at equilibrium but proceeding in the forward direction in the steady state, a result found by a method first introduced by H. G. Britton (1963, 1965) is generalized to prove that if $${{\vec J} \mathord{\left/ {\vphantom {{\vec J} {\overset{\lower0.5em\hbox{$\smash{\scriptscriptstyle\leftarrow}$}}{J} }}} \right. \kern-\nulldelimiterspace} {\overset{\lower0.5em\hbox{$\smash{\scriptscriptstyle\leftarrow}$}}{J} }}$$ is the unidirectional flux ratio, $${{\vec J} \mathord{\left/ {\vphantom {{\vec J} {\overset{\lower0.5em\hbox{$\smash{\scriptscriptstyle\leftarrow}$}}{J} }}} \right. \kern-\nulldelimiterspace} {\overset{\lower0.5em\hbox{$\smash{\scriptscriptstyle\leftarrow}$}}{J} }}$$ exp (−ΔG/RT). The conditions under which the equality or inequality applies are discussed. If the unidirectional fluxes are not in the steady state, the unidirectional flux ratio is time invariant in certain specific situations. One such important case is for chemical reaction systems with an ordered sequence of reactions. For systems with more than one pathway, $${{\vec J} \mathord{\left/ {\vphantom {{\vec J} {\overset{\lower0.5em\hbox{$\smash{\scriptscriptstyle\leftarrow}$}}{J} }}} \right. \kern-\nulldelimiterspace} {\overset{\lower0.5em\hbox{$\smash{\scriptscriptstyle\leftarrow}$}}{J} }}$$ is not constant except for special cases. These results also apply to diffusional and active transport systems.
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    Bulletin of mathematical biology 42 (1980), S. 599-600 
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    Bulletin of mathematical biology 42 (1980), S. 539-549 
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    Bulletin of mathematical biology 42 (1980), S. 551-597 
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    Notes: Abstract The nonlinear second-order difference equationx n+1=axn(1-xn−1), where 0≦x nX≦1 anda ≧1, is examined from varying points of view, analytical, numerical and geometrical. An analytic expression is obtained for an invariant attracting curveC ∞ (a) in phase space, which becomes the central object of study. This basic curve, which replaces the simple parabolic shape typical of many analogous first-order models, may have a complicated geometrical structure. As the parametera increases,C ∞(a) undergoes transformations characterized by the dynamical descriptions: stable node→stable focus→stable limit cycle →chaotic attractor. Although the limited characterization ofchaos by the appearance of nonperiodic solutions and solutions of arbitrarily large period is relied upon, this appears to be only a simplified approximation of the real behavior of solutions. Trajectories (x n, xn+1),n=0,1,…, are calculated using the related nonlinear planar mapT a(x,y)=(y,ay(1−x)), and regions of persistence and escape are described for characteristic values ofa. The study of persistence, of even more fundamental interest than the associated problems of periodicity and stability, receives special attention. We introduce a geometrical model, similar in many respects to that for the well-known analoguex n+1=axn(1−x n), but having several new and important features. It appears that as the parametera increases in the chaotic regime there are infinitely many intermittent bursts of increase in the probability that any initial point (x 0, x1) will persist in the unit square under successive iterations of the mappingT a, an unexpected property that should be of interest for applications. A discussion of the applicability of these results to population dynamics theory is given, and it is suggested that such equations might find useful application to problems in developmental biology as well.
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    Bulletin of mathematical biology 42 (1980), S. 627-645 
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    Notes: Abstract Based on the principle of minimum power, a mathematical model of the functional state of the oxygen transport system is presented. The optimization model minimizes the power expenditure of the heart, bone marrow, lung and other tissues. The model is used to determine the functional parameters of the oxygen transport system in man under both normal and varying barometric pressures. Theoretical results are compared with experimental data.
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    Bulletin of mathematical biology 42 (1980), S. 601-625 
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    Notes: Abstract A quantitative model of ion binding and molecular interactions in the lipid bilayer membrane is proposed and found to be useful in examining the factors underlying such membrane characteristics as shape, sidedness, stability and vesicle size at various cation concentrations. The lipid membrane behaves as a bilayer couple whose preferential radius of curvature depends on the expansion or contraction of one monolayer relative to the other. It is proposed that molecular packing may be altered by electrostatic repulsion of adjacent like-charged phospholipid headgroups, or by bringing two headgroups closer together by divalent cation crossbridging. The surface concentrations of each type of cation-phospholipid complex can be described by simple binding equilibria and the Gouy-Chapman-Stern formulation for the surface potential in a diffuse double layer. The asymmetric distribution of acidic phospholipids in most biological membranes can account for the differential effects of identical ionic environments on either side of the bilayer. The fraction of vesicle material which tends to have a right-side-out orientation may be approximated by a normal distribution about the mean curvature. The theory generates vesicle sidedness distributions that, when fitted to experimental results from human erythrocyte membranes, provide an alternative method of estimating intrinsic cationphospholipid dissociation constants and other molecular parameters of the bilayer. The results also corroborate earlier suggestions that the Gouy-Chapman theory tends to overestimate free counter-ion concentrations at the surface under large surface potentials.
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    Bulletin of mathematical biology 42 (1980), S. 681-689 
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    Notes: Abstract The “yellow strips” on the cuticle of the Oriental Hornet (Vespa orientalis, Hymenoptera, Vespinae), present photoelectric properties. A mathematical model for the relative changes in resistance as a photoconductive process conforms to the general model for a semiconductor with traps.
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    Bulletin of mathematical biology 42 (1980), S. 701-718 
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    Notes: Abstract Damped nonlinear oscillations in biological and biochemical systems are investigated by the extended Krylov-Bogoliubov-Mitropolskii (KBM) method. A review on the extension made by Popov to the KBM method is given and also further improvements are presented. Applications are made to models of oscillating chemical reactions (Lefever and Nicolis, 1971), FitzHugh (1961) equations, and population dynamics (Gatto and Rinaldi, 1977). Comparison to damped oscillating physical and engineering systems is made.
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    Bulletin of mathematical biology 42 (1980), S. 719-728 
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    Notes: Abstract The conditions that will allow the lumping together of several age classes in the Leslie model are investigated. We show that if the lumping is to be valid for all population distributions, then the parameters of the model must be periodic. Lumping is valid when the population is in equilibrium, but equilibrium should be tested before the model is lumped.
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    Bulletin of mathematical biology 42 (1980), S. 647-679 
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    Notes: Abstract Catastrophe theory is a mathematical theory which, allied with a new and controversial methodology, has claimed wide application, particularly in the biological and the social sciences. These claims have recently been heatedly opposed. This article describes the debate and assesses the merits of the different arguments advanced.
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    Bulletin of mathematical biology 42 (1980), S. 765-795 
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    Notes: Abstract Estimates of capillary tracer permeability calculated using multiple indicator data depend upon the particular model adopted to describe blood tissue exchange. The model proposed by Crone (1963) is appropriate when some of the injected tracer diffuses into the tissue but does not return appreciably to the bloodstream before data collection is terminated. Under these conditions extraction of tracer by the tissue depends on a single dimensionless parameter, αcap, defined as the ratio of capillary permeability surface area to water flow. The effects of finite red cell tracer permeability on the Crone model estimate of capillary permeability are examined in the present study. The results indicate that even when back diffusion from the extravascular space is negligible, significant errors in the Crone model estimate can be expected when capillary permeability is relatively high and the ratio of red cell to capillary permeability is less than unity. However, when an aliquot of blood is equilibrated with tracer prior to injection and the dimensionless capillary permeability is relatively low (i.e. αcap ≦ 0.25 for a haematocrit≦50%), the whole blood Crone model estimate of αcap will be within 10% of the actual value, irrespective of red cell permeability. Red cell-plasma exchange for commonly used tracer-organ combinations should not significantly affect Crone estimates of capillary permeability under normal physiological conditions, but may be important in low flow situations.
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    Bulletin of mathematical biology 42 (1980), S. 807-828 
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    Notes: Abstract Assuming truncated ellipsoidal geometry for the right and left ventricles, a model is developed for the myocardium enabling biventricular mechanical behavior to be studied. Employing pressure-volume data taken from normal dog hearts and from hearts in which the pulmonary artery has been banded over periods of 2–40 weeks, it is shown that: (a) right ventricular wall stresses are higher than left ventricular stresses; (b) right ventricular wall stress increases initially to a maximum after 3–4 weeks followed by a decline to normal and even subnormal levels, attaining a minimum value at 32–33 weeks; (c) left ventricular stresses behave in a similar manner, attaining their maximum and minimum levels after 7–8 weeks and 32–33 weeks respectively. These results suggest that surgical or medical therapy in patients with hypertrophied ventricles might be more appropriate during the period of wall stress reduction.
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    Bulletin of mathematical biology 42 (1980), S. 837-845 
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    Notes: Abstract In this paper we describe a mathematical model of the oscillations of the diaphragm which limits the vitreous body from the anterior segment of the human eye after the lens has been removed in a cataract operation. We study the motion of this diaphragm driven by movements of the eye. Firstly, a mathematical statement of the problem is given and then we solve the problem exactly for a given class of eye movements. From the analysis we deduce that significant oscillations of the membrane are driven by saccades and that it is the angular acceleration of the eye which causes these types of oscillations. A numerical example is given.
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    Bulletin of mathematical biology 42 (1980), S. 871-887 
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    Notes: Abstract The Lotka-Volterra system of prey-predator equations is considered with a special type of continuous time delay. In the case of equal diffusion coefficients Hopf’s bifurcation technique is used to show the existence of travelling wave train solutions for the prey-predator system.
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    Bulletin of mathematical biology 42 (1980), S. 861-870 
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    Notes: Abstract A mathematical model of prothrombin activation is being proposed which includes the feedback mechanism of thrombin and the alteration of factor V by thrombin. This model is in good agreement with experimental data for the dependence of the rate of thrombin formation on the concentrations of factors V and X a . In particular, it correctly predicts the existence and location of a maximum in both of these cases.
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    Bulletin of mathematical biology 42 (1980), S. 847-859 
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    Notes: Abstract A new model of the upper tracheobronchial tree is proposed to account for the three-dimensional nature of the airway system. In addition to the tube length, the tube diameter, and the branching angle, the model includes information on the orientation angle of each tube relative to its parent tube. The orientation angle, defined as the angle between two successive bifurcations, is useful for calculating the gravitational inclination of each tube. The information on orientation angle is further used to construct a binary coding system for identifying individual tubes in the airway tree. The proposed model is asymmetrical, but the same principles can be readily used to construct a symmetrical one.
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    Bulletin of mathematical biology 42 (1980), S. 889-897 
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    Notes: Abstract In any control system for which the number of independent controls is smaller than the number of degrees of freedom to be controlled, our choice of control in any state is restricted to a submanifold of smaller dimension than the tangent space. This simple fact has a number of important consequences for questions of biological import; we consider its implications for adaptation, for senescent phenomena and for the determination of tertiary structures of polypeptides through control of certain average properties. We also formulate the Pontryagin Maximum Principle of Optimal control theory in such a way as to inquire whether specific biodynamic systems can be regarded as optimal with respect to rate of accumulation of particular quantities of the system. We find that if this is possible, the quantity in question must play the role of a clock.
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    Bulletin of mathematical biology 42 (1980), S. 899-900 
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    Bulletin of mathematical biology 43 (1981), S. 59-67 
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    Notes: Abstract The theory of computational complexity and certain explicitly-stated hypotheses imply limitations on the information processing power of biological systems. Parallelism, special purpose organization, and analog mechanisms may provide speedup critical for life processes, but have little power in the face of exponential growth. We show that “polynomially simulatable” biological systems cannot exhibit dynamic behavior which produces the solution of an intractable problem. The argument implies that parallelism does not allow biological systems to defeat the exponential explosion, but rather is important because it allows polynomial time algorithms to be used more efficiently.
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    Bulletin of mathematical biology 43 (1981), S. 81-88 
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    Notes: Abstract A correlation matrix analysis is applied to the base sequence of MS2 and ϕX174 in comparison with sets of simulated sequences with different degrees of constaint Significant differences between a codified sequence, and a statistical one in terms of the “correlation matrix” for sets of different length cannot be found. This result is analysed in terms of nucleotide sequences with different levels of informational content.
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    Bulletin of mathematical biology 43 (1981), S. 101-109 
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    Notes: Abstract A method of calculating the volume of a tree distal to a cut at the origin of a branch, using branching, diameter and length ratios, has been developed. The method was applied to bronchial tree casts from human, dog, sheep, hamster, and rat lungs. It was found that the exponenta in the equation weight=k×diameter a is approximately equal to 3.0 in sheep lung casts, as found by Hooper (1977), but it is greater than 3.0 in casts from the other four species.
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    Bulletin of mathematical biology 43 (1981), S. 111-116 
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    Notes: Abstract In this note we examine a continuous time version of a compartmental model introduced in a discrete time setting by S. R. Bernard. The model allows for more than one particle to leave the system at any time. This introduces additional randomness into the system, over the pure death system and this is reflected in the variance function.
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    Bulletin of mathematical biology 43 (1981), S. 89-99 
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    Notes: Abstract The mean first passage time for free diffusion can be derived directly by solving a simple analogue steady state problem. In this problem the diffusion starting region is considered as a time independent source of diffusing particles and the diffusion target assumes the behaviour of a perfectly absorbing sink. It is shown here that the transit time between the source and the sink, which in this particular problem is equal to the ratio between the holdup of the system and the total flux, is identical to the Brownian movement concept of the mean first passage time for free diffusion. This established identity considerably facilitates the derivation and investigation of the timing of diffusion in complicated structures such as those commonly found in living organisms.
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    Bulletin of mathematical biology 43 (1981), S. 121-123 
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    Bulletin of mathematical biology 43 (1981), S. 117-120 
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    Bulletin of mathematical biology 43 (1981), S. 201-211 
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    Notes: Abstract In this paper three stochastic models are developed for a class of two-compartment systems to analyse the randomness of the leaving process of the particles in the system. Results in closed form for the distribution of the leaving process of the particles in the system are given both for general and exponential sojourn time distributions and also in association with forward recurrence time distributions with and without Poisson input.
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