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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 4 (1989), S. 247-252 
    ISSN: 1476-5535
    Keywords: Sterilization ; Bioreactor ; Media ; R0 ; F0
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Sterilization of bioreactor media, to destroy viability of the indigenous microbial population, is normally accomplished by autoclaving, or heating with pressurized steam. However, simultaneous chemical changes in media can also be expected to result from the high temperatures. A kinetic procedure involving on-line computer calculation of heat input, designated asF 0 values, was previously developed to estimate sterility achievement. A similar kinetic procedure, based on a general purpose Arrhenius ‘pseudo’ rate equation and designated asR 0 values, has now been designed to evaluate, and control the effects of temperature and heating time on chemical reactions occurring in the media. Data are presented indicating thatR 0 may be a useful parameter for reducing variability in culture metabolism and ‘scale-up’ when these variations result from different nutrient concentrations produced by non-standard heating during media sterilization in stirred bioreactors.
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  • 2
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 275-278 
    ISSN: 1476-5535
    Keywords: Aflatoxin ; Bioassay ; Cell growht ; Bacterium ; Density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Eight species of bacteria were incubated in culture media containing 10 μg/ml aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), or aflatoxin G2 (AFG2). Their culture density at 20°C was determined at four and eight days (d) after inoculation. In all species of bacteria studied (Bacillus cereus, Proteus mirabilis, Erysipylothrix rusiopathie (insidiosa), Streptococcus fecalis, Staphylococcus epidermis, Klebsiella pneumoniae, Micrococcus spp., andEscherichia coli), AFB1, AFB2 and AFG2 substantially decreased culture sizes at 4 d, but not at 8 d. InB. cereus andP. mirabilis, culture sizes were increased by AFB1, AFB2, and AFG2 at 8 d post inoculation. These results indicate that AFB1, AFB2, and AFG2 suppressed initial growth of these species in vitro, while later growth in some species was either unaltered or enhanced.
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  • 3
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 299-306 
    ISSN: 1476-5535
    Keywords: Bioaccumulation ; Germanium ; Sensitivity ; Tolerance ; Toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The toxicity of germanium dioxide (GeO2) to 21 bacterial and 13 yeast strains was investigated in liquid broth medium to obtain information on strains tolerant to high (1 to 2 mg/ml) GeO2 concentrations.Arthrobacter sp. NRC 32005,enterobacter aerogenes NRC 2926,Klebsiella aerogenes NCTC 418 andPseudomonas putida NRC 5019 were tolerant to 1 mg/ml GeO2.Bacillus sp. RC607 was able to grow in the presence of 2 mg/ml GeO2 at pH 10 in broth culture. The yeastsCandida guilliermondii, Candida shehatae andPachysolen tannophilus were the most sensitive to GeO2 as evidenced by their diminished growth rates at a GeO2 concentration as low as 0.1 mg/ml. None of the yeast strains tested exhibited growth in the presence of 1 mg/ml GeO2. The high pH of the medium containing germanium may be partially responsible for the growth inhibition of the yeast cultures. Select bacterial cultures previously exposed to 1 mg/ml GeO2 could tolerate and grow better at 2 mg/ml GeO2, suggesting the existence of very efficient adaptive mechanisms. The pH of the medium could modulate GeO2 tolerance and this effect was found to be strain-dependent.
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  • 4
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 325-331 
    ISSN: 1476-5535
    Keywords: Clostridium genetics ; Clostridium beijerinckii ; Clostridium acetobutylicum ; Protoplast regeneration ; L-colony
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Protocols for protoplast formation, L-colony cultivation, and regeneration ofClostridium beijerinckii NRRL B-592, B-593 andC. acetobutylicum ATCC 10132 were developed. Two osmotically reinforced media were formulated. Protoplasts of B-592, B-593, and ATCC 10132 grew as cell wall-deficient forms (L-colonies) when plated on the first medium (BLM) and continued to do so through at least 3 passages on this medium. The second (BRM) permitted the L-colonies to regenerate cell walls after transfer to this medium. TransferredC. beijerinckii B-592 L-colonies reverted to bacillary colonies at a frequency of 25%. Likewise, L-colonies of B-593 andC. acetobutylicum ATCC 10132 could be regenerated at frequencies of 7.0 and 8.6%, respectively. Thus, these procedures are suitable for genetic engineering of these industrial microorganisms using protoplast manipulation techniques.
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  • 5
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 341-347 
    ISSN: 1476-5535
    Keywords: Auerobasidium ; Color variants ; Xylanase ; Hemicellulase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The yeast-like fungusAureobasidium is a promising source of xylanase (EC 3.2.1.8) with an exceptionally high specific activity. For enzyme production in volumes of several liters, xylose was the preferred carbon source and inducer. Xylanase in clarified cultures was concentrated by reversible adsorption to cation-exchange matrix to 5% of the initial volume, and recovered at nearly 2 million IU/1. Selective conditions permitted 97% recovery of xylanase with a 1.8-fold enrichment in specific activity, to 70% of purity. The predominant xylanase species (20 kDa) was subsequently purified to 〉99% of homogeneity by gel filtration chromatography. Purified enzyme exhibited an isoelectric point of 8.5, and specific activity of 2100 IU/mg under optimal conditions, determined to be pH 4.5 and 45°C. The activity of purified enzyme was specific for polymeric xylan.
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  • 6
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 375-402 
    ISSN: 1476-5535
    Keywords: Toxicity ; Organotins ; Tin ; Methiltins ; Butyltins ; Tributyltin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Organotins are used for industrial and agricultural purposes and in antibiologic agents. They are significantly more toxic than inorganic tins, and eventually reach the environment where they can be toxic to a wide variety of organisms. Particular attention has been given to tributyltins which are highly toxic components of antifouling paints. Realization that the molecular species of organotin influences fate and effects of organotins led to development of sensitive methods for quantifying individual molecular species. Even though such methods are now available, little information has been obtained on the ability of microorganisms to bioaccumulate tin compounds. Trisubstituted alkyl and aryltins (R3Sn's) are more toxic than disubstituted compounds (R2Sn's) while monosubstituted organotins (RSn's) are still less toxic. R4Sn's are toxic only if they are metabolized to R3Sn's. Among trisubstituted compounds propyl-, butyl-, pentyl-, phenyl-, and cyclohexyl Sn's are generally the most toxic to microorganisms. Toxicity in the R3Sn series is related to total molecular surface area of the tin compound and to the octanol:water partition coefficient,K ow, which is a measure of hydrophobicity; a highK ow indicates greater hydrophobicity and predicts greater toxicity. Care must be taken when testing the toxicity of tin compounds, for a number of biological, physical and chemical factors can influence the apparent toxicity. Although little is known of the effects of tin compounds on microbial processes, a number of bacterial processes can be inhibited by organotins and all relate to membrane functions. They include effects on energy transduction, solute transport and retention and oxidation of substrates. Very little is known of how organotins exert their toxic effects on algae and fungi; Information on effects on chloroplasts and mitochondria stems principally from animal systems and from higher plants. Triorganotins act against chloroplasts and mitochondria by causing swelling, by acting as ionophores and by acting against ATPase, while diorganotins appear to act by binding to dithiol groups on enzymes and cofactors. Nucleic acids do not seem to be affected at environmentally relevant concentrations. Virtually nothing is known of the action of tin compounds on microbial enzymes, but resistant mutants are easy to obtain and should facilitate work to understand modes of microbial interaction with tin compounds and mechanisms of resistance.
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  • 7
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 419-428 
    ISSN: 1476-5535
    Keywords: Bacillus ; Paper and board machines ; Starch degrading enzymes ; Cellulase ; Proteases ; Slimicides ; Food packaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Aerobic spore-forming bacteria were found dominant in the microflora of food packaging paper and board. Twenty-five strains of bacteria belonging to the genusBacillus were isolated from these paper and board machines, papermaking chemicals, and final products of papermaking. Nineteen strains were analyzed for production of α-amylase, α-glucosidase, glucoamylase, pullulanase, β-glucanase, carboxymethyl cellulase, and caseinase, and also for resistance towards industrial biocides. pH and temperature optima for the activity of the enzymes were determined. All strains were found to produce one or more of the enzymes studied. The amylolytic enzymes of most strains had high temperature optima for activity. Vegetative cells of all strains were found very resistant towards the different commercial slimicides used in paper and board mills. This property together with the ability to survive through the dry end of the machine to the final board and paper, and the production of enzymes degrading papermaking chemicals makes these bacteria potentially harmful in paper and board mills.
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  • 8
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 441-446 
    ISSN: 1476-5535
    Keywords: DNS hybridization ; Gene probe ; Environmental survival ; Pseudomonas cepacia AC1100 ; Alcaligenes A5
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effectiveness of gene probe methods for tracking genetically engineered microorganisms (GEMs) in the environment was tested by inoculating nutrient-supplemented freshwater microcosms withAlcaligenes A5 (a naturally occurring 4-chlorobiphenyl degrader) orPseudomonas cepacia AC1100 (a genetically engineered 2, 4, 5 T-degrader) and following the fates of the introduced bacterial populations. Colony hybridization of the viable heterotrophic bacterial populations and dot blot hybridization of DNA recovered from the total microcosm microbial communities showed persistence of bothAlcaligenes A5 andP. cepacia AC1100 in the microcosms in the presence and absence of the xenobiotic substrates that these organisms biodegrade. Although there was a gradual decline in the added populations, both of the bacterial populatins were still detected in the microcosms two months after their introduction into the microcosms. Addition of 2, 4, 5-T enhanced the survival ofP. cepacia AC1100 — and 4-chlorobiphenyl addition resulted in increased levels ofAlcaligenes A5. The results indicate that both organisms may persist for very long periods in freshwater habitats.
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  • 9
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 181-187 
    ISSN: 1476-5535
    Keywords: Nocardia amarae ; Surface tension ; Hydrocarbon affinity ; Montmorillonite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Cultures ofNocardia amarae give rise to cell-stabilized foams in a laboratory scale foaming apparatus. The organism produces a surfactant and the cells are very hydrophobic; factors which, in terms of froth flotation theory, are essential for foam production and transport of the cells from the aqueous to the bubble phase. The addition of montmorillonitic clay to the culture prior to foaming prevents foam stabilization. The results obtained suggest the formation of a salt-dependent, reversible, bacterium-montmorillonite complex which prevents transport of cells to the bubble phase.
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  • 10
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 195-207 
    ISSN: 1476-5535
    Keywords: Coal leaching ; Desulfurization ; Thiobacilli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The leaching of six Eastern coals was investigated using experimental coal columns subjected to simulated leaching events. Measurements of CO2 assimilation and specific enrichment cultures indicated that the microbial communities of all leachates were dominated by iron- and sulfur-oxidizing chemoautotrophic bacteria. Comparison of CO2 assimilation rates in leachates and core samples of leached coal indicated that most chemoautotrophs remained within coal columns during leaching. Mean numbers of chemoautotrophic bacteria in leachate samples were correlated with concentrations of dissolved iron and sulfate. Leachates from unwashed, run-of-mine coals contained more chemoautotrophs and more iron and sulfate than did leachates from washed, final product coals. After several leachings, the ratio of sulfur oxidizers to iron oxidizers tended to increase. These data suggest that the chemoautotrophic community of final product coals may be pyritelimited. Aerobic heterotrophs constituted a minor component of the microbial community in leachates from the six coals and their abundance and metabolic activity were apparently not influenced by the beneficiation history of the coal. Changes in rates of acetate metabolism may have been related to microbial succession within the heterotrophic community of coal columns. In all leachates, rates of tritiated methylthymidine assimilation were correlated with rates of acetate incorporation but not with CO2 assimilation, even though autotrophs dominated the microflora. Thus, thymidine assimilation rates appear to reflect activities or growth of mainly heterotrophic microorganisms in leachate.
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  • 11
    ISSN: 1476-5535
    Keywords: Metabolism ; Acetate ; Alginate ; Carbon balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Some metabolic properties of both suspended and immobilized aerobically and anaerobically growingEscherichia coli cells were investigated. Metabolic activity was found to be substantially different whenE. coli cells were immobilized in alginate. Cells grown immobilized in alginate, and then released from the gel, synthesized 1.6 (aerobic growth) and 4.9 (anaerobic growth) times as much β-galactosidase per cell in response to induction as did suspended cells. Under both aerobic and anaerobic conditions, the cell yield from glycerol for immobilized cells was half that for suspended cells. At specific growth rates that were not significantly different from those of suspended cells, immobilized cells consumed glycerol at twice the rate of suspended cells. Immobilized cells produced elevated quantities of acetate, pyruvate, and lactate. Interpretation of these findings is discussed in terms of the kinetics of energy metabolism and the regulation of inducible protein synthesis inE. coli.
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  • 12
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 267-274 
    ISSN: 1476-5535
    Keywords: Glucose isomerase ; Immobilization ; K-carrageenan ; Glucose ; Fructose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary ImmobilizedArthrobacter cells (NRRL-B-3728) were used for continuous isomerization of glucose to fructose in a bioreactor system. The system utilized stationary phase (55h) cells (2.2×109 CFU/ml saline) immobilized onto K-carrageenan (3% w/v) beads [cells were heated at 65°C for 10 min to inactivate endogenous proteolytic enzymes]. Immobilized-cell preparations were hardened using three different glutaraldehyde systems. Glutaraldehyde (0.2 M) treated-immobilized cells (pH 7.0, 5°C for 30 min) exhibited good gel strength and high glucose isomerase activities. Maximal bioreactor isomerization of 44% was achieved when a buffered feedstock containing 40% glucose was fed into the column (60°C) at a flow rate of 0.2 ml/min. The biological half-life of glucose isomerase activities in this system was 400 h. Scanning electron microscopy revealed large numbers of cells distributed within the beads. A thin layer surrounding the beads following glutaraldehyde treatment was mainly due to cross-linking reactions between cell proteins and glutaraldehyde. This layer prevented leaking of cells during continuous isomerization reaction.
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  • 13
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 289-298 
    ISSN: 1476-5535
    Keywords: Biodegradation ; Landfarming ; Metal-working coolants ; Waste-oil emulsions ; On-site oil disposal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The use of land treatment for disposal of a dilute waste oil emulsion generated by an aluminum rolling industry was investigated. Major components of the waste, identified by gas chromatography and mass spectrometry, were linear and branched (C12−C25) and fatty acid emulsifiers (primarily, isomers of oleic acid). Hexadecane and pristane were readily biodegraded in vitro when added to soil collected from the waste disposal site. Hydrocarbons and fatty acids extracted from the waste were similarly, biodegraded, however, the rate of decomposition may have depended on the history of waste applications to soil collected from the land treatment site. The apparent half-life of resolvable waste hydrocarbons and fatty acids was 9.5 days in soil which had received waste applications averaging 25.4l m−2 wk−1. In contrast, soil receiving either 50.8l m−2 wk−1 or no waste application during summer 1987 apparent exhibited half-lives of 28.1 and 60.3 days, respectively. Waste components were restricted to the upper 48 cm of the soil cores collected from the disposal site. Core samples also provided evidence for biodegradation of hydrocarbons and fatty acids as well as an accumulation of other compounds not readily resolvable by gas chromatography
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  • 14
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 315-323 
    ISSN: 1476-5535
    Keywords: Sugar uptake ; Yeast ; Brewer's wort
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary When glucose and fructose are fermented separately, the uptake profiles indicate that both sugars are utilized at similar rates. However, when fermentations are conducted in media containing an equal concentration of glucose and fructose, glucose is utilized at approximately twice the rate of fructose. The preferential uptake of glucose also occurred when sucrose, which was first rapidly hydrolyzed into glucose and fructose by the action of the enzyme invertase, was employed as a substrate. Similar results were observed in the fermentation of brewer's wort and wort containing 30% sucrose and 30% glucose as adjuncts. In addition, the high levels of glucose in the wort exerted severe catabolite repression on maltose utilization in theSaccharmyces uvarum (carlsbergensis) brewing strain. Kinetic analysis of glucose and fructose uptake inSaccharomyces cerevisiae revealed aK m of 1.6 mM for glucose and 20 mM for fructose. Thus, the yeast strain has a higher affinity for glucose than fructose. Growth on glucose or fructose had no repressible effect on the uptake of either sugar. In addition, glucose inhibited fructose uptake by 60% and likewise fructose inhibited, glucose uptake by 40%. These results indicate that glucose and fructose share the same membrane transport components.
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  • 15
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 403-408 
    ISSN: 1476-5535
    Keywords: Bromochlorodimethylhydantion ; Legionella pneumophila ; Industrial cooling water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Free residual chlorine and bromine can be generated in water from bromochlorodimethylhydantoin (BCDMH). Efficacy of chlorine from inorganic sources has been studied extensively, but there is much less information on the efficacy of bromine againstL. pneumophila; only a few efficacy studies of organically-derived. halogen appear in the literature and the results from different studies conflict or are difficult to interpret. This paper describes the efficacy of halogen from BCDMH against planktonic, pure cultureL. pneumophila in an industrial cooling water. There was no difference in efficacy between halogen derived from organic or inorganic sources in controlled laboratory experiments. Effective doses in laboratory studies cannot be translated directly to field applications because of significant differences in the microbiology. However, the data suggest that disinfection (〉99.9% reduction in viability within 10 min) of planktonic, pure cultureL. pneumophila can be achieved with about 1 ppm free residual halogen (expressed as chlorine) from BCDMH in a typical industrial cooling water.
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  • 16
    ISSN: 1476-5535
    Keywords: Coriolus versicolor ; Wood-decay fungus ; Polyphenol oxidase ; Substrate specificity ; de novo Synthesis ; Partial purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Coriolus versicolor, a white-rot Basidiomycete, secretes cellulolytic and ligninolytic enzymes as well as polyphenol oxidase (PPO). Whereas the former degrade wood polymers, the latter can convert diphenols to diquinones and oligomerize syringic acid, a lignin derivative. Certain phenolic compounds can serve as disease-resistance factors controlling the proliferation of wood-decay fungi within host tissues. BecauseC. vesicolor can be ‘batch-cultured’, overproduction and enhanced secretion of enzymes of biological and commercial interests are feasible. Reported here are the results of attempts to define the timed appearances of intracellular and extracellular PPO, to assess substrate specificity as well as distinguish synthesis versus activation of intracellular PPO and to partially purify extracellular PPO. These efforts were to provide data enabling cell-free synthesis of PPO, cloning of the gene(s) for the oxidase and the establishment of its subcellular route of secretion. Whereas two protein peaks (6 and 12 days in a 16 day time-course) were observed for dialyzed mycelial homogenates, the homogenates' PPO specific activity rose between 4 and 12 days and then declined. Total extracellular protein content climbed from 6 to 15 days for dialyzed growth medium and the medium's PPO specific activity rose at 4 days post-inoculation and except at 9 days increased linearly to 15 days. When aliquots of dialyzed 12 and 15 day media were added to PPO assay mixtures containing catechol and either syringic or gallic acids, statistically significant differences in PPO specific activity between phenolic substrates were noted. Supplementation of cultures with 1.91 μg cycloheximide ml growth medium−1 (control, growth medium only) together with 0.5 μCi [14C]-leucine revealed that cycloheximide inhibited PPO activity and suppressed [14C]-leucine incorporation into TCA-insoluble cytoplasmic protein. As for PPO partial purification, growth medium dialysis followed by 0–30% (NH4)2SO4 fractionation and subsequent 12 000×g dialyzate centrifugation yielded a 3.27-fold enhancement in PPO specific activity within the 12 000×g supernatant. Chromatography of the latter upon DEAE-Sephadex indicated that PPO exchanged with the DEAE counterion as it could be eluted with high ionic strength salt. These results suggest that: the occurrences of intracellular and extracellular PPO are time-dependent, intracellular PPO is de novo synthesized, the preferred substrate for extracellular PPO appears to be catechol and extracellular PPO can be partially purified by a combination of dialysis and ammonium sulfate fractionation as well as possibly DEAE chromatography and/or Sephadex G-150 gel filtration.
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  • 17
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 429-434 
    ISSN: 1476-5535
    Keywords: Sampling ; Biofilm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Quantitative sampling of periphyton from natural substrates is difficult and uncommon due to the nonhomogenous and irregular nature of most natural substrates. This paper describes an experimentally verified method for quantitative sampling of periphyton directly from the relatively homogenous and regular upper deck of a cooling tower.
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  • 18
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 71-75 
    ISSN: 1476-5535
    Keywords: Lactobacillus bulgaricus ; Whey permeate ; Peptide average molecular weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The batch fermentation of whey permeate to lactic acid was improved markedly by the addition of enzymehydrolyzed whey protein. Acid concentrations greater than 90 g/l were achieved at a productivity of 4.3 g/l per h and a 98% substrate use. Cell mass concentration reached 6 g/l. The acid productivity achieved is somewhat higher than that typical for fermentation of whole whey. The process economics, based on in-house hydrolyzate preparation, look promising. Presented in this paper are the experimental results showing the effects of hydrolyzate concentration on acid and cell mass production.
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  • 19
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 127-133 
    ISSN: 1476-5535
    Keywords: Salt-tolerant enzyme ; Heat-tolerant enzyme ; Depolymerase ; Lyase ; Oligosaccharide ; Viscosity breaker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A new high-temperature, salt-tolerant xanthanase suitable as an enzymic viscosity breaker for xanthanbased hydraulic fracture fluids was obtained by soil enrichment growth on xanthan gum incubated at 45°C in the presence of 3% NaCl. The mixed culture produces exoenzymes functional up to 65°C in the presence of salts. Degradation products include the pyruvic acetal of mannose and branched oligosaccharides derived from cleavage of main-chain β-(1→4)-d-glucosyl linkages. Release of the terminal pyruvic acetal ofd-mannose leads to oligosaccharide products that evidently contain the ene-4,5-unsaturated glucuronic acid residue.
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  • 20
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 145-154 
    ISSN: 1476-5535
    Keywords: Disinfectant ; Hypochlorite ; Chlorine dioxide ; Iodine ; Hydrogen peroxide ; Glutaraldehyde ; Quaternary ammonium compound ; Phenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The activity of eleven disinfectants againstStaphylococcus aureus, Pseudomonas aeruginosa, andSaccharomyces cerevisiae was determined using a method based on the A.O.A.C. germicidal and detergent sanitizer assay. Based on the activity against the test organisms after 30-and 60-s exposures to each disinfectant, the disinfectant containing chlorine dioxide had the highest biocidal activity in this assay, on a mg/l basis. In addition, a disinfectant containing sodium hypochlorite and a disinfectant containing sodium chlorite performed well, at concentrations below label specifications. The results illustrate the importance of testing disinfectants in the context of their intended use.
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  • 21
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 173-180 
    ISSN: 1476-5535
    Keywords: Arabinan-degrading enzyme ; Arabanase ; p-Nitrophenyl-α-l-arabinosidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A variety of thermophilic organisms andBacillus species were screened in shake flask culture for arabanase andp-nitrophenyl-α-l-arabinosidase activities. Highest arabanase activity was produced by strains ofThielavia terrestris andSporotrichum cellulophilum. Thermoascus aurantiacus and severalBacillus species were most active producers of arabinosidase. Arabinosidases fromBacillus strains had pH optima in the range 5.9–6.7. pH optima of fungal arabinosidases ranged from ≤2.9 to 6.7.Bacillus arabanases had neutral pH optima, whereas fungal arabanases had pH optima in the range 3.7–5.1. In general, arabinosidases were found to be relatively thermostable, retaining 〉70% activity for 3 h at 60°C. TheT. aurantiacus enzyme retained 98% activity at 70°C after 3 h.Bacillus arabanases were relatively unstable. All fungal arabanases except theT. aurantiacus enzyme were fully denatured at 70°C after 3 h.
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  • 22
    ISSN: 1476-5535
    Keywords: Isocoumarin ; Phosphodiesterase ; Enzyme inhibitor ; cGMP phosphodiesterase ; Secondary metabolite
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Three isocoumarins have been isolated from a strain ofStreptoverticillium sp. and all inhibit the calmodulin-sensitive cyclic guanosine 3′,5′-monophosphate phosphodiesterase (EC 3.1.4.17, Boehringer Mannheim). Two of the compounds, 6,8-dihydroxy-7-methoxy-3-methyl isocoumarin and 6,7,8-trihydroxy-3-methyl isocoumarin have previously been isolated fromStreptomyces. The third fermentation product, 6,8-dihydroxy-3-methyl isocoumarin, was also found as a metabolite ofCeratocystis minor, a fungal species associated with the blue stain disease of pine [2,3].
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  • 23
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 215-229 
    ISSN: 1476-5535
    Keywords: Microbial enhanced oil recovery ; Microbial selective plugging ; Petroleum microbiology ; Permeability ; Biofouling
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The ability of indigenous populations of microorganisms in Berea sandstone to improve the volumetric sweep efficiency and increase oil recovery by in situ growth and metabolism following the injection of nutrients was studied. Cores of differing permeabilities connected in parallel without crossflow and slabs of sandstone with differing permeabilities in capillary contact to allow crossflow were used. The addition of a sucrosenitrate mineral salts medium stimulated the growth and metabolism of microorganisms in the sandstone systems. This resulted in a preferential decrease in permeability in the core or slab with the higher initial permeability, diverted flow into the lower-permeability core or slab and improved the volumetric sweep efficiency. Injectivity into the slab with the lower initial permeability in the crossflow system increased during subsequent nutrient injections. Thus, microbial selective plugging does occur in laboratory systems that have the complex flow patterns observed in petroleum reservoirs without losing the ability to inject fluids into the formation. In situ microbial growth and metabolism increased oil recovery 10 to 38% of the original oil in place. Biogenic gas production accompanied oil production, and much of the gas was entrained within the produced oil suggesting that gas production was an important factor leading to increased oil recovery. Quantitation of the amount of phospholipid in the core confirmed that microbial growth preferentially occurred throughout the core with the higher initial permeability. These data showed that in situ microbial growth in the high-permeability regions improved not only the volumetric sweep efficiency but also the microscopic oil displacement efficiency.
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  • 24
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 255-266 
    ISSN: 1476-5535
    Keywords: Sulfate-reduction ; Anaerobic biodegradation ; Ground water ; Bacterial enrichment ; Phenol ; Cresol
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Sulfate-reducing bacterial enrichments were obtained from a shallow anoxic aquifer for their ability to metabolize eithero-, m-, orp-cresol. GC/MS and simultaneous adaptation experiments suggested that the anaerobic decomposition ofp-cresol proceeds by the initial oxidation of the aryl methyl group to formp-hydroxybenzoic acid. This intermediate was then converted to benzoic acid. Benzoic acid and a hydroxybenzaldehyde were also found in spent culture fluids from ano-cresol-degrading enrichment culture. This result, in addition to others, suggested thato-cresol may also be anaerobically degraded by the oxidation of the methyl substituent. An alternate pathway for anaerobicm-cresol decomposition might exist. Enrichment cultures obtained with eitherp- oro-cresol degraded both of these substrates but notm-cresol. In contrast, am-cresol enrichment culture did not metabolize theortho orpara isomers. Anaerobic biodegradation in all enrichment cultures was inhibited by molybdate and oxygen, and was dependent on the presence of sulfate as a terminal electron acceptor. The stoichiometry of sulfate-reduction and substrate depletion by the various enrichment cultures indicated that the parent cresol isomers were completely mineralized. This result was confirmed by the conversion of14C-labeledp-cresol to14CO2. These results help clarify the fate of alkylated aromatic chemicals in anoxic aquifers.
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  • 25
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 279-288 
    ISSN: 1476-5535
    Keywords: Lead ; Organotins ; Organoleads ; Tin ; Tributyltin ; Yeasts
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Four methods were used to screen nine organotin and two organolead compounds for toxicity to 29 yeasts, representing 10 genera. Center well diffusion plates were useful in comparing the sensitivity of yeasts to the most toxic organometals but were not useful for comparisons between compounds because of differences in diffusion rates and lack of sensitivity. Two-layer diffusion plates (density gradient plates) were also of limited use for comparisons between compounds but provided quantitative information on toxicity and allowed comparisons between organisms. Two-dimensional diffusion plates were useful for estimating the effect of pH on organometal toxicity. Release of K+ from cell suspensions measured using a K+-electrode provided quantitative information and allowed comparisons between compounds and organisms. The presence of 3% NaCl in cell suspensions decreased the rates and extent of organotin-induced K+ release. Yeasts varied in their sensitivity from strain to strain, but tributyltin was the most toxic compound tested. Mono- and dimethyltins were the least toxic. Triphenyltin, dibutyltin, monobutyltin, trimethyltin, triethyltin, diethyllead, diethyltin, and dimethylleads showed intermediate toxicity, but triphenyltin and monobutyltin were the most toxic among the group.
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  • 26
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 1-5 
    ISSN: 1476-5535
    Keywords: Xylose isomerase ; Enzyme expression ; thermally inducible ; Hollow fiber bioreactor ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary TheEscherichia coli xylose isomerase (EC 5.3.1.5) has been expressed under the control of a thermal inverting promotor system (att-nutL-p-att-N block) and its performance in a hollow fiber bioreactor measured. The conversion of xylose to xylulose was inversely proportional to the flow rate and the system operated up to 60°C. The maximum conversion efficiency observed was 19.05% at 55°C.
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  • 27
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 13-17 
    ISSN: 1476-5535
    Keywords: Streptococcus lactis ; UV irradiation ; Liquid holding recovery ; Photoreactivation
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Repair of ultraviolet-light-induced DNA damage inStreptococcus lactis has been examined. The wild-type strain and its derivative Lac− possess a dark repair system (maximal increase in survival of 4-fold). Enzymatic photoreactivation exists in the two strains but a weaker photoreactivability was found in the Lac− derivative (4 and 2-fold, respectively). Concomitant reduction of UV-induced mutagenesis (Rifr marker) was also studied during these two repair phenomena. The absence of dark repair after saturation of photoreactivation suggests that photoreactivation is much more efficient with pyrimidine dimers as substrate.
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  • 28
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 37-42 
    ISSN: 1476-5535
    Keywords: Exported recombinant protein ; Recombinant protein ; β-Galactosidase ; Leader peptide ; Thaumatin
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary To produce and direct the export inStreptomyces lividans of the sweet plant protein thaumatin, thaumatin II cDNA was fused in the correct reading frame to the β-galactosidase leader peptide, under the control of the β-galactosidase promoter and ribosome binding site. The export of the recombinant thaumatin may allow the correct formation of the thaumatin disulfide bonds. The recombinant thaumatin was purified from the medium on an S-Sepharose column and detected with western blots by sheep α-thaumatin antibodies. The recombinant thaumatin was the same size as authentic thaumatin and changed position on an acrylamide gel in response to reduction by 2-mercaptoethanol in the same manner.
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  • 29
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 55-64 
    ISSN: 1476-5535
    Keywords: Rifampicin ; Bacitracin ; Exopolysaccharide ; Productivity ; Viscosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Two classes of mutants ofXanthomonas campestris B1459 were isolated that accumulate more xanthan gum than the parental wild-type in culture broths of shake flask cultures and both batch and fed-batch fermentations. The first mutant class was resistant to the antibiotic rifampicin and accumulated, on average, about 20% more xanthan gum than wild-type. The second mutant class, a derivative of the first, was resistant to both bacitracin and rifampicin, and accumulated about 10% more xanthan than its parent. On a weight basis, the viscosities of the polysaccharides made by each strain were not distinguishable. Only a subset of the drug-resistant mutants were overproducers of xanthan. The biochemical basis for the overproduction of xanthan by the mutant strains has not been determined. Both new strains served as recipients for recombinant plasmids bearing ‘xanthan’ genes and further augmented the effects of multiple copies of those genes on xanthan productivity.
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  • 30
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 77-80 
    ISSN: 1476-5535
    Keywords: Lactobacillus bulgaricus ; Endoprotease ; Peptide average molecular weight
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The batch fermentation of whey permeate to lactic acid was improved by supplementing the broth with enzyme-hydrolyzed whey protein. Hydrolyzates prepared with endoprotease were more stimulatory to acid production rates than were those prepared with exo/endo protease. The effect of hydrolyzate average molecular weight on acid production is presented. Results show that the hydrolyzate having an average molecular weight of 700 is the most stimulatory to acid production rates.
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  • 31
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 121-125 
    ISSN: 1476-5535
    Keywords: Clostridium acetobutylicum ; Granulose ; Capsule ; Exopolysaccharide ; Oxido-reduction
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The production of granulose (an intracellular reserve polygranule), capsule and exopolysaccharide was investigated in a synthetic medium in which the oxido-reduction level was modified by the addition of acetic or butyric acid. After addition of the acids, granulose synthesis increased from 150 to 300 mg glucose equivalents ·1−1 and capsular synthesis decreased by 25%. Exopolysaccharide production was unchanged under these conditions. A hypothesis that attributes a role to the polymer in the oxido-reduction sequences is discussed.
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  • 32
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 135-144 
    ISSN: 1476-5535
    Keywords: Xanthomonas campestris ; Xanthan gum ; Secretion ; Cellulase ; Amylase ; Pathogenesis
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Mutants ofXanthomonas campestris B 1459 were isolated that are defective in secretion of both cellulase and amylase. Both enzymes accumulated in the periplasmic space. The defects in secretion of cellulase or amylase were partly overcome by introducing into the mutants specific multiple copies of DNA cloned fromX. campestris, and presumed to code for cellulase or amylase enzymes. The mutant strains also showed reduced amounts of extracellular pectinase and protease activities, as if the mutants were generally defective for secretion of extracellular enzymes. The mutants showed reduced pathogenesis for turnip seedlings. The secretion-defective mutants may allow production of xanthan gum with reduced cellulose, pectin, protein and starch-degrading enzyme activities, thereby allowing more widespread mixing of microbially produced xanthan gum with these commercially important water-soluble polymers.
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  • 33
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 155-161 
    ISSN: 1476-5535
    Keywords: Rhizopus arrhizus ; Fungal growth ; Filamentous growth ; Hyphal morphology ; Fermentation medium
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Rhizopus arrhizus biomass attached itself to fermentor walls, baffles and impellers when grown in casein/ glucose media. In shake flasks, dispersed filamentous growth was produced in media containing certain concentrations of glucose and soya flour. Other media tested produced pelleted or clumpy growth. Medium initial pH did not affect morphology type. Dispersed growth could not be obtained by addition of detergents, oils and polymers to a clear glucose/soya peptone medium. Addition of maize solids to this medium resulted in dispersed growth which occurred even in the presence of calcium, which in most media caused pellet formation. Mycelia appeared to bind to the maize particles and use these as growth centres thereby preventing pellet or clump formation. Mycelial pellets appeared to originate either from a single spore or by interaction of branched hyphae from different spores. Medium composition and macro-morphology type correlate with differences in hyphal structures.
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  • 34
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 189-193 
    ISSN: 1476-5535
    Keywords: Gastroenteritis ; Cytotoxin ; Food safety
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The lethality of 16 clinical or food isolates ofAeromonas hydrophila was assessed by determination of LD50 (i.p.) in mice and goldfish. In mice LD50 values for the variousA. hydrophila strains were similar, ranging from 1.2–21.0×108 cells/animal. A wider range of LD50 values, 0.03–11.8×108 cells/animal, was observed with goldfish. Lethality was not correlated between the two test animals. Further, cytotoxic response in Y-1 adrenal cells did not correlate with lethality in either test animal. It appears that lethality is not a good measure of potential enterotoxigenicity, but may be useful in assessing the invasive character of isolates causing systemic infections in immunocompromised hosts.
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  • 35
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 231-237 
    ISSN: 1476-5535
    Keywords: Avermectin ; Streptomyces avermitilis ; Biosynthesis
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The biosynthesis of avermectins was studied further inStreptomyces avermitilis MA5502 by feeding experiments with labeled precursors.13C-NMR analysis of the compounds biosynthesized from [2-13C]acetate, [1,2-13C2]acetate, [3-13C]propionate and [2,3-13C2]propionate confirmed that the aglycone of avermectins is made from seven intact acetate and five propionate units. Feeding experiments with [1-13C]2-methylbutyrate and [1-13C]isobutyrate have shown that 2-methylbutyrate and isobutyrate are immediate precursors of the starter units of the polyketide chains of avermectin ‘a’ and ‘b’ components, respectively. The3H/14C doublelabeling experiments suggest that the two oleandrose moieties are derived from glucose.
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  • 36
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 7-12 
    ISSN: 1476-5535
    Keywords: Photoreactivation ; Streptomyces lividans ; UV survival ; Visible light
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Biological systems can repair damage induced in their DNA by ultraviolet light (UV). Most cells contain at least three DNA repair pathways, each of which has a marked effect on UV survival. Excision repair and recombinational (postreplication) repair are light-independent whereas photoreactivation (PR), whether enzyzmatic or photochemical, is light-dependent. The specificity of photoreactivation for UV-induced DNA damage allows it to be used as a tool for examining whether premutational DNA lesions are preferred sites for photoreversal; it therefore plays an important role in mutagenesis studies. Evidence is presented here that PR occurs in a time-dependent fashion in three strains ofStreptomyces lividans 66. The effect appears to be independent of temperature and is observed only when PR treatment is given after UV irradiation. The present experiments do not discriminate between enzymatic and photochemical protection.
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  • 37
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 29-35 
    ISSN: 1476-5535
    Keywords: Methane-utilizing bacteria ; Halogenated hydrocarbons ; Trichloroethylene ; Chloroform ; Dichloromethane
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Soil was taken from the top 10 cm of a soil column that removed halogenated aliphatic hydrocarbons in the presence of natural gas. This soil was used as an enrichment inoculum to determine that the removals seen in the soil column were in fact of a microbiological nature. Methane served as the source of carbon and energy and was consumed immediately by the enrichments. After several transfers of the enrichments, a stable consortium of at least three bacterial types was obtained. The predominant bacterium was a non-motile, gram-negative coccus. This stable consortium was able to remove chlorinated methanes, ethanes, and ethylenes when grown with methane and oxygen in the headspace. Methane was required for the removals to be observed. Acetylene inhibited the removals, which further suggests the involvement of methanotrophs. Benzene and toluene were removed by the mixed culture with or without methane in the headspace. Fatty acid analysis of the mixed culture resulted in a profile that indicated that the predominant organism was a type II methanotroph. This study provides further evidence that methanotrophic bacteria are capable of cometabolizing a wide range of chlorinated methanes, ethanes, and ethylenes.
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  • 38
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 49-53 
    ISSN: 1476-5535
    Keywords: l-Phenylacetyl carbinol ; Saccharomyces cerevisiae ; Yeast ; Benzaldehyde ; Biotransformation
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The rate of production ofl-phenylacetyl carbinol bySaccharomyces cerevisiae in reaction mixtures containing benzaldehyde with sucrose or pyruvate as cosubstrate was investigated in short 1 h incubations. The effect of yeast dose rate, sucrose and benzaldehyde concentration and pH on the rate of reaction was determined. Maximum biotransformation rates were obtained with concentrations of benzaldehyde, sucrose and yeast of 6 g, 40 g and 60 g/l, respectively. Negligible biotransformation rates were observed at a concentration of 8 g/l benzaldehyde. The reaction had a pH optimum of 4.0–4.5. Rates of bioconversion of benzaldehyde and selected substituted aromatic aldehydes using both sucrose and sodium pyruvate as cosubstrate were compared. The rate of aromatic alcohol production was much higher when sucrose was used rather than pyruvate.o-Tolualdehyde and 1-chlorobenzaldehyde were poor substrates for aromatic carbinol formation although the latter produced significant aromatic alcohol in sucrose-containing media. Yields of 2.74 and 3.80 g/l phenylacetyl carbinol were produced from sucrose and pyruvate, respectively, in a 1 h reaction period.
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  • 39
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 81-84 
    ISSN: 1476-5535
    Keywords: Ethanol fermentation ; Wheat starch ; Saccharomyces cerevisiae ; immobilization ; Continuous dynamic immobilized biocatalyst bioreactor ; Biocatalyst bioreactor
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A simple and efficient method of conversion of wheat starch B to ethanol was investigated. Employing a two-stage enzymatic saccharification process, 95% of the wheat starch was converted to fermentable sugars in 40 h. From 140 g/l total sugars in the feed solution, 63.6 g/l ethanol was produced continuously with a residence time of 3.3 h in a continuous dynamic immobilized biocatalyst bioreactor by immobilized cells ofSaccharomyces cerevisiae. The advantages and the application of this bioreactor to continuous alcoholic fermentation of industrial substrates are presented.
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  • 40
    ISSN: 1476-5535
    Keywords: Fermentation development ; Cholecystokinin
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    Notes: Summary The natural product asperlicin is the first nonpeptide antagonist of cholecystokinin isolated from a microbial source. At discovery, production of asperlicin by the original soil isolate ofAspergillus alliaceus was between 15 and 30 mg/l. Selection of natural variants ofA. alliaceus, use of Plackett & Burman and Simplex experimental designs; formulation of synthetic media; amino acid supplementation of production media; analysis of complex nitrogen sources for their amino acid content; evaluation of promising media in fermentors; substitution of glycerol for glucose as a carbon source and rational mutant selection all contributed to titer increases to 〉900 mg/l.
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  • 41
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 109-120 
    ISSN: 1476-5535
    Keywords: Ground water ; Biodegradation ; Hydrocarbon ; Adaptation ; Subsurface ; Creosote ; Microorganism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The microbial ecology of pristine, slightly contaminated, and heavily contaminated subsurface materials, and four subsurface materials on the periphery of the plume at an abandoned creosote waste site was investigated. Except for the unsaturated zone of the heavily contaminated material, mineralization of glucose (13.5 ppb) indicated a metabolically active microflora in all subsurface materials. However, mineralization (〈40%) of naphthalene, phenanthrene, and 2-methylnaphthalene was observed in contaminated material and material from the periphery of the plume, but not in pristine material. Pentachlorophenol was mineralized in material from the periphery of the plume. Inorganic and organic nutrient amendments and changes in pH and temperature did not increase the extent of mineralization of the aromatic compounds. An array of organic compounds found in creosote were biotransformed in contaminated ground water; however some compounds were still detected after 7 months of incubation. The data suggest that the subsurface microflora in slightly and heavily contaminated subsurface materials and materials from the periphery of the plume has adapted to degrade many compounds found in creosote.
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  • 42
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 163-171 
    ISSN: 1476-5535
    Keywords: Bacteriocin ; Plasmid curing ; Phenotype ; Pediococcus acidilactici
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Pediococcus acidilactici strains E, F and H isolated from fermented sausages produced bacteriocins which were protein in nature and inhibitory to a variety of spoilage and pathogenic microorganisms often encountered in foods. These strains harbored two to three plasmids ranging in size from 7.4 to 40.2 megadaltons. Curing experiments and plasmid profile analysis indicated the involvement of plasmid DNA with bacteriocin activity in all three strains. Carbohydrate fermentation and antibiotic resistance phenotypes did not appear to be associated with bacteriocin plasmids. Both bacteriocin activity and resistance determinants were linked in strain H and mediated by a 7.4-megadalton plasmid, whereas in strains E and F these two traits were not linked.
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  • 43
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    Meteorology and atmospheric physics 33 (1984), S. 11-20 
    ISSN: 1436-5065
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    Topics: Geography , Physics
    Description / Table of Contents: Zusammenfassung Die Energieflüsse wurden im Dischma-Tal in der Nähe von Davos in 1970 m Höhe vom 6. bis 15. August 1980 gemessen. Sie waren ein Teil einer Sonderuntersuchung mikroskaliger atmosphärischer Bedingungen in einem alpinen Tal. Die allgemeine Funktion ϕ H konnte von einem Satz von Profilmessungen der Temperatur, der Feuchtigkeit und der Windgeschwindigkeit abgeleitet werden. Sie entspricht der Beziehung, die auch über homogenem Terrain gefunden worden ist.
    Notes: Summary Surface energy fluxes were measured in the Dischma-valley near Davos, Switzerland at 1970 m from August 6th to August 15th, 1980. They are part of a case study for the microscale atmospheric conditions of an alpine valley. The universal function ϕ H could be derived from the whole set of profile measurements of temperature, humidity and wind speed. It corresponds to the relation which was found over homogeneous terrain.
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    Meteorology and atmospheric physics 33 (1984), S. 31-37 
    ISSN: 1436-5065
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    Topics: Geography , Physics
    Description / Table of Contents: Zusammenfassung Die nichtlineare Wechselwirkung zwischen großskaligen Rossby-Wellen wird mit Anwendung eines spektralen Modells einer barotropen Atmosphäre in sphärischer Geometrie einer Betrachtung unterzogen. Es wurde gefunden, daß es, wenn eine willkürliche Zahl von zonalen Komponenten in Betracht gezogen wird, möglich ist, die nichtlineare Energie- und Entropie-Strömung zwischen Rossby-Wellen vorherzusagen, deren Wechselwirkung mit einfacher triadischer Resonanz nicht vorhergesagt werden kann. Mit Beziehung auf die Ergebnisse einer Arbeit von Baines wird gezeigt, daß eine solche Art von zonal mittlerer, nichtlinearer Energieströmung imstande ist, einige wichtige Besonderheiten der numerischen Integration von verkürzten Modellen mit einer großen Zahl von Komponenten zu erklären, und als Folgerung an einigen Besonderheiten der großskaligen Zirkulation schuld sein könnte.
    Notes: Summary The non-linear interaction between large-scale Rossby waves is considered making use of a spectral model of a barotropic atmosphere in spherical geometry. It is found that, if an arbitrary number of zonal components is taken into account it is possible to predict the non-linear energy and enstrophy flux between Rossby waves whose interaction cannot be predicted by single triadic resonance. It is shown, referring to the results of a paper by Baines, that such kind of, zonally mediated, non-linear energy flux is capable of explaining some relevant features of numerical integrations of truncated models with a large number of components and, could, as a consequence be responsible of some features of the large-scale circulation.
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    Meteorology and atmospheric physics 33 (1984), S. 49-53 
    ISSN: 1436-5065
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    Topics: Geography , Physics
    Description / Table of Contents: Zusammenfassung Es wird ein einfacher theoretischer Vergleich zwischen dem Phänomen der Entwicklung von Mischungsschichten in Laboratoriumsexperimenten und in wirklichen geophysikalischen Situationen durchgeführt. Die relative Wichtigkeit von Inversionschichten in der Atmosphäre wird im Kontrast zur vernachlässigbaren Rolle dargelegt, die die Inversionsschicht bei Laboratoriumsexperimenten spielt, solange der Effekt von ausgestrahlten Schwerwellen in Betracht gezogen wird.
    Notes: Summary A simple theoretical comparison is carried out between the phenomenon of the evolution of mixed layers in laboratory experiments and in actual geophysical situations. The relative importance of the inversion layers in the atmosphere is evidentiated in contrast with the negligible role played by the inversion layer in laboratory experiments as long as the effect of radiated gravity waves is taken into account.
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    Meteorology and atmospheric physics 33 (1984), S. 61-67 
    ISSN: 1436-5065
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    Topics: Geography , Physics
    Description / Table of Contents: Zusammenfassung Es werden die Verhältnisse im Luft-Meer-Grenzbereich im frühen Entwicklungsstand einer Depression über der östlichen zentralen Arabischen See während der Monsun MONEX-79 durch Analyse der Schiffsbeobachtungsdaten über Luft- und Meerestemperaturen, Luftdruck an der Oberfläche, Seegang und Wind untersucht. Diese Analyse zeigte eine ausgesprochene Zunahme der Temperaturdifferenz zwischen Meer und Luft (2–4 °C) und diese Zunahme erweist sich klar als durch eine Abnahme der Lufttemperatur verursacht. Die änderungen in der Temperaturdifferenz zwischen Meer und Luft und des Luftdrucks verlaufen in zueinander entgegengesetzter Phase. Einige einleuchtende Mechanismen für die beginnende Entwicklung und die Bewegung der Depression werden auch besprochen.
    Notes: Summary The air-sea interface properties during the early stages of formation of a depression over East Central Arabian Sea during summer MONEX are examined by analyzing the ship data for air and sea temperatures, sea level pressures, sea state numbers and wind fields. Analysis of the data revealed a pronounced increase in sea-air temperature difference (2–4 °C) and this increase in the value is considered, obviously, to be due to a drop in the air temperature. The variations in the sea-air temperature difference and surface pressure are in opposite phase to each other. Some of the plausible mechanisms for the incipient development and movement of the depression are also discussed.
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    Meteorology and atmospheric physics 34 (1984), S. 1-19 
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    Description / Table of Contents: Zusammenfassung Einige natürliche Experimente werden analysiert, um Gleichgewichtswerte einer Folgefunktion der Lufttemperatur in Bodennähe und eines Rückkopplungsfaktors für die Erdatmosphäre zu erhalten. Der erste der beiden Parameter beinhaltet die änderung der Bodenlufttemperatur, hervorgerufen durch eine Änderung in der an der Oberfläche absorbierten Strahlungsenergie, und besitzt eine Größenordnung von 0.1 K (Wm−2)−1. Der letztere der beiden Parameter beschreibt das Verhältnis zwischen den durch Rückkopplung erzeugten änderungen in der von der Erdoberfläche aufgefangenen Strahlungsenergie und den anfänglichen, oder primären, Änderungen in dieser Energie. Dieser Parameter besitzt eine Größenordnung von 1.25. Diese zwei Zahlen besagen, daß die Maximalerwärmung, die man von einer Verdopplung des CO2-Gehaltes der Erdatmosphäre von 300 auf 600 ppm erwarten kann, nur ungefähr 0.1 K beträgt. Dieser Wert ist so klein, daß er womöglich völlig durch die vom CO2 bewirkte Reduktion der zur Erdoberfläche durchgelassenen Sonnenstrahlung aufgehoben wird.
    Notes: Summary Several natural experiments are analyzed to yield equilibrium values of a surface air temperature response function and a feedback factor for Earth’s atmosphere. The former parameter, the change in surface air temperature induced by a change in radiant energy absorbed at the surface, is demonstrated to have a value of about 0.1 K (Wm−2)−1; while the latter parameter, the ratio of feedback-induced change in radiant energy to the surface of the Earth divided by an initial or primary change in radiant energy to the Earth’s surface, is demonstrated to have a value of about 1.25. These two numbers imply that the maximum warming to be expected from a doubling of Earth’s atmospheric CO2 concentration from 300 to 600 ppm is only about 0.1 K, a result so small as to possibly be completely counter-balanced by the CO2-induced reduction of solar radiation transmission to the Earth’s surface.
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    Meteorology and atmospheric physics 34 (1984), S. 39-48 
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    Description / Table of Contents: Zusammenfassung Mit Verwendung von Jahres- und Monatswerten des lokalen Zonalindex werden die Zirkulationsänderungen in den letzten hundert Jahren im Gebiet A (20°W–10°E) und im Gebiet B (10°E–40°E) untersucht. Für das Gebiet A hat sich ergeben: 1. In der Teilperiode 1873–1902 waren blockierende Systeme vorherrschend und der Typ einer westlichen Zirkulation kam weniger oft vor. 2. In der Teilperiode 1903–1932 herrschte in 80% der Jahre eine abnormale Situation in Richtung zu zonaler Westströmung hin vor. 3. Die dritte Periode (1933–1972) war vom Gesichtspunkt der Intensität durch zeitweise zonale Strömung charakterisiert. Für das Gebiet B zeigte sich, daß die Zirkulation entsprechend einem niedrigen Index vorherrschend meridional war. Im Gebiet B kam eine zonale östliche Strömung in 26% der Jahre vor.
    Notes: Summary Using annual and monthly values of the local zonal index, we study the circulation change during the past hundred years for the area A (20°W–10°E) and area B (10°E–40°E). For the area A the results are: 1) During the subperiod (1873–1902) blocking systems were predominant and westerly type of circulation was less frequent. 2) For the subperiod (1903–1932) an abnormal situation prevailed in 80% of the years, towards the direction of W zonal flux. 3) The third subperiod (1933–1972) was characterized from the point of view of intensity of zonal flux as intermediate. For the area B showed that the prevailing circulation was actually meridional or “Low Index”. Also for the area B the easterly zonal circulation was 26%.
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    Meteorology and atmospheric physics 34 (1984), S. 49-63 
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    Description / Table of Contents: Zusammenfassung Die in dieser Arbeit vorgelegten Ergebnisse wurden mit Anwendung von zwei physikalisch begründeten Modellen der Sonnenstrahlung und einem Energiebudget eines Hanges abgeleitet. Es wurden über 10 Breitengrade der Nordhemisphäre Amerikas gemittelte klimatische Beobachtungsdaten als Eingaben zur Berechnung der Änderungen der Oberflächentemperaturen und der Differenzen zwischen Oberflächen- und Lufttemperaturen als Funktion verschiedener Hangneigungen (0 bis 90 Grad) und Orientierungen (süd-, west- und nordschauend) verwendet. Wir haben als Kontraste unbewachsene, und grasrbewachsene Landschaften vergleichend untersucht. Es wurden keine einfachen Beziehungen zwischen den die kurz- und langwellige Strahlungsabsorption betreffenden Faktoren, den Komponenten des Energiebudgets und den Tendenzen der Oberflächentemperatur gefunden. Eine große Verschiedenheit besteht zwischen den Oberflächentemperaturen verschiedener Hänge und Orientierungen in einer bestimmten Breite. Diese Ergebnisse werden für hinreichend dafür gehalten, die meisten der möglichen Gegensätze in einem Nord-Süd-Querschnitt in einer realen Welt zu erfassen.
    Notes: Summary The results presented in this paper are derived from the continuation of the use of two physically-based models of solar radiation and a steady state slope energy budget [1]. Again, climatic observational inputs (Northern Hemisphere Americas), averaged in 10-degree latitude bands, were utilized in order to compute the changing surface temperatures and differences between surface and air temperatures as functions of varying slope angles (0 to 90 degrees) and orientations (south-, west-, and north-facing). We continued to examine two contrasting landscapes: barren and grassy. No simple relations were found among the factors regarding shortwave and longwave radiative absorption, components of the energy budget, and surface temperature trends. A great diversity existed among surface temperatures of different slopes and orientations at any particular latitude. This was accentuated in the higher latitudes. These findings were assumed general enough to encompass most of the possible contrasts encountered in a real-world north-south transect.
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    Meteorology and atmospheric physics 34 (1984), S. 273-285 
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    Description / Table of Contents: Zusammenfassung Jüngst veröffentlichte Vorschriften zur Berechnung des menschlichen Wärmestresses in einer städtischen Umgebung bezogen die Beiträge des Himmels, der Gebäudewände und der Straßen ein. Der Bruchteil der Gesamtlangwellenstrahlung, die von einer Person aus jeder dieser Flächen empfangen wird, kann durch deren entsprechende Ansichtsfaktoren beschrieben werden. Bisher waren jedoch die Vorschriften zur Bestimmung derartiger Ansichtsfaktoren in einer städtischen Umgebung nur mangelhaft beschrieben. Die vorliegende Arbeit empfiehlt eine Technik, mittels der die Ansichtsfaktoren von Himmel, Wänden und Straße für eine Person in einer städtischen Straßenschlucht berechnet werden können, ungeachtet der komplexen Natur der Horizontlinie der Stadt.
    Notes: Summary Recent procedures for estimating human heat-stress in urban environments have included the contribution of longwave radiation from surrounding sky, walls and streets. The fraction of the total longwave radiation received from each of these surfaces by a person may be described by their respective view-factors but, hitherto, techniques by which view-factors may be determined in the urban environment have been deficient. This paper describes a technique by which sky, wall and street view-factors may be calculated for a person in an urban canyon, regardless of the complexity of the city skyline.
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    Meteorology and atmospheric physics 23 (1974), S. 1-8 
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    Description / Table of Contents: Zusammenfassung Daten der thermischen Struktur der Atmosphäre, die mit dem mehrere Strahlungsbänder umfassenden SIRS-Instrument (Satellite Infrared Radiometer Spectrometer) des Nimbus III gemessen wurden, wurden zur Berechnung von Parametern der allgemeinen Zirkulation in der Nordhemisphäre herangezogen. Die thermische Struktur unterhalb der 10mb-Fläche wird mittels einer Regressionsmethode bestimmt, welche die Schichtdicke zwischen Isobarenflächen als abhängige Veränderliche benützt. Die täglich berechneten Parameter der allgemeinen Zirkulation umfassen die zonale mittlere und die wirbelpotentielle Energie sowie die zonale und wirbelkinetische Energie. Unabhängig davon wurden dieselben Parameter von Netzdaten des National Meteorological Center (NMC) bestimmt. Ein Vergleich zwischen den beiden Kollektiven von Daten zeigt, daß Maxima, Minima und allgemeine Tendenzen in beiden Berechnungen gut übereinstimmen. Die mittels SIRS berechneten Energiewerte stellen allerdings eine Unterschätzung dar. Die Energieveränderungen in der mittleren Stratosphäre während des Zusammenbruches des Polarwirbels werden als Beispiel angeführt.
    Notes: Summary General circulation parameters in the northern hemisphere are calculated using atmospheric thermal structure obtained from Nimbus III SIRS multi-channel radiance information. The thermal structure up to 10 mb is obtained by using a regression technique with thickness between pressure levels as the dependent variable. General circulation parameters calculated on a daily basis include zonal and eddy available potential energy, and zonal and eddy kinetic energy. A second set of calculations is performed using National Meteorological Center (NMC) grid data. A comparison of the two sets of calculations indicates that, although the energies calculated from the SIRS-derived structure underestimate the actual energies, maxima, minima and trends are well identified. An example of mid-stratospheric energy changes during a breakdown of the polar-night vortex is also given.
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    Meteorology and atmospheric physics 23 (1974), S. 13-27 
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    Description / Table of Contents: Zusammenfassung Der Gesamtozongehalt kann auf globaler Basis täglich von Satelliten aus gemessen werden, die die Erde umkreisen. Solche Messungen wurden durch den Nimbus-III-Satelliten durchgeführt. Ein Vergleich der zeitlichen und longitudinalen Veränderlichkeit der von der Stratosphäre emittierten langwelligen Strahlung in 60° südlicher Breite mit dem Gesamtozongehalt zeigte, daß niedrige (hohe) Strahlungswerte gut mit niedrigen (hohen) Gesamtozonwerten übereinstimmen. Die Phasengeschwindigkeit der nach Osten wandernden “Ozonwellen” ist in der Winterhemisphäre am größten. Die Ostwärtsbewegung nimmt gegen niedere Breiten der Winterhalbkugel ab. In äquatorialen Breiten scheinen sich die Wellen langsam gegen Westen zu bewegen. Im Sommer der Nordhemisphäre wandern die Wellen langsam gegen Osten. Die Intensität der “Ozonwellen” ändert sich ebenfalls mit der geographische Breite. Die zeitlichen und räumlichen Änderungen im Gesamtozongehalt sind hauptsächlich an die Bewegungen troposphärischer Drucksysteme geknüpft. Die starken, horizontalen Ozongradienten, welche häufig in der Winterhemisphäre beobachtet wperden, hängen mit intensiven baroklinen Zonen in der unteren Stratosphäre und der oberen Troposphäre zusammen, die sich ebenfalls nach Osten hin bewegen. Unter Annahme einer gleichförmigen zonalen Grundströmung konnten in der Ozonverteilung Rossby-Wellen festgestellt werden, deren Wellenlänge zwischen 2500 und 3700 km variierte.
    Notes: Summary With total ozone sensors on earth-orbitung satellites the ozone distribution over the entire earth may be measured daily. The conclusions from some of the Nimbus III measurements are discussed in this paper. A comparison of time-longitude variations of stratospheric radiance values at 60°S with values of the total ozone indicated that low (high) radiance values correspond very closely with the low (high) total ozone amounts. The speed at which these ozone ‘waves’ progress eastward is greatest in the winter hemisphere. The speed of eastward progression decreases as one approaches the lower latitudes in the winter hemisphere. In the equatorial region the progression of the ozone ‘waves’ appears to be slowly westward. In the Northern Hemisphere the waves progress slowly eastward during summer. The intensity of the ozone ‘waves’ was also observed to change with latitude. These temporal and spatial fluctuations are related mainly to the motion of dynamic tropospheric systems. The extremely tight ozone gradients, which may be seen in the winter hemisphere data, have been shown to be associated with strong baroclinic zones in the lower stratosphere and upper troposphere, which are moving eastward. Assuming uniform zonal velocity we found Rossby wavelengths that varied from 2500 to 3700 km.
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    European journal of nutrition 23 (1984), S. 12-19 
    ISSN: 1436-6215
    Keywords: Energie ; Wärmeproduktion ; Realimentation ; Nüchternumsatz ; Erhaltungsumsatz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary A metabolism trial with six adult sows was conducted to study the effect of fasting and realimentation on heat production, energy retention, and utilization of energy. Complete balance measurements by indirect calorimetry (CN-method, RQ-method) were carried out from the 8th to the 10th day of fasting, and thereafter from the 1st to the 7th day and from the 33rd to the 36th day of the realimentation phase. Previous to fasting and during the time of refeeding the energy supply was adapted to maintenance requirement. Heat production rose by 20% after refeeding the animals. This heat increment corresponded to 2.2 MJ/kg feed dry matter, or 12% of the energy intake, respectively. The stimulation of heat production induced by refeeding occurred spontaneously within one day. After the first day of refeeding a plateau was reached, which in the course of the steady energy supply was still present even after the 5th week of realimentation. Residual effects of fasting in the mode of a compensatory heat production were not observed. Therefore, a constant utilization of efficiency of metabolizable energy for maintenance was estimanted at 82%. The change of body energy during the entire experimental period resulted in a loss of 198 MJ corresponding to about 5% of the body weight.
    Notes: Zusammenfassung In einem Gesamtstoffwechselversuch mit 6 ausgewachsenen Sauen wurden Wärmeproduktion und Energieretention bei Nüchterung und nachfolgender kontrollierter Realimentation untersucht. Die quantitative Stoffwechselmessung mit Hilfe der indirekten Kalorimetrie (CN-Methode und RQ-Methode) fand am 8.–10. Hungertag und am 1.–7. sowie 33.–36. Tag der Wiederfütterung statt. Die Nahrungszufuhr war vor und nach der Hungerphase auf den energetischen Erhaltungsbedarf ausgerichtet. Die Wärmeproduktion lag in der Realimentation gegenüber der Hungerphase um 20% höher. Dieser Wärmezuwachs entsprach 2,2 MJ/kg Futtertrockenmasse bzw. 12% der Energieaufnahme. Der Energiewechsel adaptierte sich innerhalb eines Tages an die Situation der Wiederfütterung. Danach blieb die Wärmebildung bzw. die Energieretention bis Ende des Versuchs auf einem konstanten Niveau. Nachwirkungen der Nüchterung im Sinne einer kompensatorisch veränderten Wärmeproduktion traten nicht auf. Die energetische Verwertung der umsetzbaren Energie für Erhaltung berechnete sich auf 82%. Die über die gesamte Versuchszeit erstellte Energiebilanz ergab ein Defizit der Körperenergie von 198 MJ, entsprechend etwa 5% des Körpergewichtes.
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    European journal of nutrition 23 (1984), S. 31-40 
    ISSN: 1436-6215
    Keywords: endogener Kotstickstoff ; bakterieller Stickstoff ; Guarmehl ; 15N-Tracer-Technik ; Ratten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The metabolic fecal nitrogen was determined in 8 young rats (90–100 g body weight) using a single oral dose of 75 mg15N-glycine. Four rats were fed a diet containing 8 % guar and 4 rats received a control diet without guar. Compared to the control group, apparent protein digestibility and nitrogen balance were significantly lower in the guar group. After an initial steep rise the15N-elimination in feces and urine (days 2–8 after15N-glycine application) followed an exponential curve in at least 2 phases, with increasing half-time values. The proportion of metabolic fecal nitrogen to total fecal nitrogen was calculated from the15N-atom-% excess, determined daily in urine and in feces. This calculation is based on the assumption that the specific15N-activity (15N-atom-% excess) in urine represents the specific15N-activity of the endogenous nitrogen pool. The addition of guar raised the proportion of metabolic fecal nitrogen from 51 % (control) to 73 %, corresponding to an increase in total fecal nitrogen loss (24 mg) observed in the guar-fed animals. This indicates that the rise in fecal nitrogen loss caused by guar is due almost entirely to enhanced secretion of endogenous nitrogen and not to undigested food protein. Based on the analysis of 2,6-diamino-pimelic acid in the feces of 4 rats the proportion of bacterial nitrogen in the feces was calculated. It amounted to 19.8 % of total fecal N in the control group, and to 30.1 % in the guar group, or to 39.4 % and 44.4 % of metabolic fecal nitrogen respectively. Obviously the increased dietary fiber intake also caused a rise in the amount of metabolic fecal nitrogen, which is derived from gastro-intestinal secretions and epithelial tissue.
    Notes: Zusammenfassung Zur Bestimmung der endogenen Stickstoffausscheidung im Kot wurde acht jungen Ratten (90 bis 100 g Körpergewicht) eine orale Dosis von 75 mg15N-Glycin verabreicht. Vier Ratten erhielten eine 8%ige Guarmehldiät und vier Ratten eine Kontrolldiät ohne Guarmehlzusatz. Gegenüber der Kontrollgruppe wurde eine signifikante Verringerung der scheinbaren Proteinverdaulichkeit und der N-Bilanz in der Guarmehlgruppe festgestellt. Nach einem anfänglichen steilen Anstieg verlief die15N-Elimination in Kot und Urin vom 2. bis 8. Tag nach der15N-Glycin-Applikation exponentiell in mindestens zwei Phasen mit steigender Halbwertszeit. Aus den experimentell ermittelten Tageswerten des15N-Atom-%-Überschusses in Urin und Kot wurde der Anteil des endogenen Stickstoffs im gesamten Kotstickstoff berechnet. Hierbei wurde vorausgesetzt, daß die spezifische15N-Aktivität des Urins (15N-Atom-%-Überschuß) dem15N-Markierungsgrad des endogenen Stickstoff-Pools entspricht. Durch Guarmehlzusatz erhöhte sich der Anteil des endogenen Kotstickstoffs von 51% (Kontrollgruppe) auf 73%, was einem Anstieg von 10 mg N/Tag auf 32 mg N/Tag entspricht. Diese Differenz von 22 mg N/Tag ist vergleichbar mit dem um 24 mg/Tag erhöhten N-Verlust im Kot der Tiere in der Guarmehlgruppe. Demnach sind die durch Guarmehl verursachten erhöhten N-Verluste fast ausschließlich durch eine vermehrte endogene N-Sekretion und nicht durch unverdautes Nahrungsprotein bedingt. Mit Hilfe der 2,6-Diamino-pimelinsäure-Analyse des Kots von vier Ratten wurde der Anteil des aus Darmbakterien stammenden Kot-N bestimmt. Er betrug 19,8 % (Kontrollgruppe) bzw. 30,1% (Guarmehlgruppe) des Gesamt-Kot-N bzw. 39,4% (Kontrollgruppe) und 44,4 % (Guarmehlgruppe) des endogenen Kot-N. Hieraus ist zu folgern, daß die erhöhte Ballaststoffzufuhr auch eine vermehrte Ausscheidung an endogenem Stickstoff in Form von N-haltigen Sekreten und Epithelzellen des Magen-Darm-Traktes bewirkt hat.
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    European journal of nutrition 23 (1984), S. 58-65 
    ISSN: 1436-6215
    Keywords: Feststoffsubstrate ; Kolonienbildung von Mikroorganismen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. Of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: Bacillus subtilis, B. cereus, Streptococcus sanguis, S. saprophyticus, S. faecalis, Escherichia coli, Micrococcus flavus, Actinomyces viscosus, Veillonella sp., Enterobacter liquefaciens, Yersinia enterocolitica. Lactobacillus casei behaved neutral. Proteus vulgaris, Y. enterocolitica and Chromobacterium violaceum grow on certain media only in the presence of solid particles. The yeasts Endomyces lactis, Saccharomyces cerevisiae, Rhodotorula mucilaginosa and Cryptococcus nigricans did not respond to solid particles during colony formation. The kind of protein — animal (meat) or vegetable (soy beans) — did not influence the behaviour of the microorganisms in these experiments.
    Notes: Zusammenfassung Es wurde festgestellt, daß die Bakterien an Kontaktstellen mit sowohl organischen als auch anorganischen Feststoffmaterialien anders wachsen als auf reinen Agarnährböden. Von 16 ad hoc gewählten Bakterienstämmen reagierten folgende Spezies mit intensiverer Kolonienbildung in sinkender Reihenfolge: Bacillus subtilis, B. cereus, Streptococcus sanguis, S. saprophyticus, S. faecalis, Escherichia coli, Micrococcus flavus, Actinomyces viscosus, Veillonella sp., Enterobacter liquefaciens, Yersinia enterocolitica. Lactobacillus casei verhielt sich neutral. Proteus vulgaris, Y. enterocolitica und Chromobacterium violaceum wachsen auf bestimmten Nährböden nur in Anwesenheit der Festpartikel. Die Hefen Endomyces lactis, Saccharomyces cerevisiae, Rhodotorula mucilaginosa und Cryptococcus nigricans zeigten bei der Kolonienbildung keine Reaktion auf die Feststoffpartikel. Die Art des Proteins in Nährböden — tierisch (Fleisch) oder pflanzlich (Sojabohnen) — beeinflußte das Verhalten der Mikroorganismen in diesen Untersuchungen nicht.
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    European journal of nutrition 23 (1984), S. 104-112 
    ISSN: 1436-6215
    Keywords: vitamin A ; inner ear ; review ; vitamin A deficiency ; hearing loss
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Since the first characterization and description of vitamin A this is used in otolaryngologic therapy for different forms of hearing disorders, and its relation to the inner ear is subject of investigation. Animal experiments and clinical studies were done to clarify the significance of vitamin A for the function of hearing. Besides this there were a lot of observations describing correlations between vitamin A metabolism and hearing loss. Recent investigations showed that vitamin A is present in high concentrations in the inner ear and stored there. Morphological experiments revealed different and in some way contradictory results, but they showed that vitamin A seems to be essential for inner-ear morphogenesis.
    Notes: Zusammenfassung Seit der Entdeckung des Vitamin A und ersten Beschreibung seiner Wirkung wird dieses auch in der Otorhinolaryngologie als Therapeutikum bei den unterschiedlichsten Formen von Hörstörungen eingesetzt und seine Wirkungsweise am Innenohr erforscht. So wurden sowohl tierexperimentelle als auch klinische Studien durchgeführt, um die Bedeutung des Vitamins für die Funktion des Hörorgans zu klären. Daneben wurden eine Reihe von Beobachtungen mitgeteilt, die einen Zusammenhang zwischen Störungen des Vitamin-A-Stoffwechsels und gleichzeitig auftretenden Hörstörungen beschrieben. Neuere Untersuchungen haben ergeben, daß Vitamin A in hohen Konzentrationen im Innenohr vorkommt und dort auch gespeichert werden kann. Morphologische Untersuchungen führten zu den unterschiedlichsten Ergebnissen und haben gezeigt, daß Vitamin A für die Morphogenese des Innenohres von Bedeutung zu sein scheint.
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    European journal of nutrition 23 (1984), S. 276-282 
    ISSN: 1436-6215
    Keywords: starvation ; brain ; lipid metabolism ; dietary lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Der Einfluß verschiedener Nahrungsfette auf den Lipidstoffwechsel des Gehirns während Hunger wurde untersucht. Albinoratten wurden 8 Tage lang mit Dalda, Senföl oder Erdnußöl gefüttert. Einige Tiere blieben anschließend 3 und 5 Tage lang ohne Nahrung. Die Gesamtlipide und die Galaktolipide wurden durch Nahrungsfette und durch Hunger nicht beeinflußt, während die Phospholipide unabhängig von der Art des Nahrungsfetts signifikant zunahmen. Cholesterin nahm in den mit Dalda und mit Erdnußöl gefütterten Gruppen zu und nahm ab während des Hungerns. In der Hungerperiode im Anschluß an die Fettzufuhr war der Einbau von 1-14C-Acetat in Lipide von Gehirnschnitten nach Erdnußöl höher als nach Dalda. Nach Senföl war der Einbau nur gering.
    Notes: Summary In this study the effects of the dietary fat sources, viz., Dalda, mustard oil and groundnut oil in the brain lipid metabolism during starvation has been investigated. To find out this, these oils were fed to albino rats for 8 weeks followed by 3 and 5 days of starvation. Total as well as galactolipids of brain were not affected by dietary oils and starvation, whereas phospholipids of brain were significantly increased by fasting irrespective of the dietary fats. On the other hand, cholesterol was found to be increased in the groups fed with Dalda and groundnut oil and to be significantly decreased during fasting experiments. Incorporation of (1-14C)acetate into lipid of the brain slices of rats fed different experimental diets than followed by starvation revealed that the uptake of radioactivity was higher in the group fed with groundnut oil, followed by Dalda. Low uptake was observed in the group fed with mustard oil.
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  • 58
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    European journal of nutrition 28 (1989), S. 56-75 
    ISSN: 1436-6215
    Keywords: free radicals ; cataracts ; antioxidant vitamins ; vitamin C ; vitamin E ; β-carotene ; Katarakte ; antioxidative Vitamine ; Vitamin C und E ; Betakarotin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Die Augenlinse kann durch einfallendes Licht und Sauerstoff photooxidativ so geschädigt werden, daß eine Trübung bzw. ein Katarakt entsteht. Sauerstoffradikale schädigen nicht nur die Kristalline, spezialisierte Linsenproteine, die Aggregate bilden und präzipitieren, sondern sie greifen auch proteolytische Enzyme an, deren Funktion es wäre, die geschädigten Proteine zu eliminieren. Neben einem enzymatischen Abwehrsystem gegen Sauerstoffradikale, bestehend aus Superoxiddismutase, Katalase und Glutathionperoxidase, enthält die Linse die antioxidativen Vitamine C und E und evtl. Betakarotin. Tierversuche an verschiedenen Spezies haben sowohl in vitro als auch in vivo eine Schutzwirkung gegen lichtinduzierte Kataraktbildung aufgezeigt. Eine ähnliche Wirkung war gegen Zucker- und Steroidkatarakte nachweisbar. Epidemiologische Studien am Menschen haben gezeigt, daß Personen mit vergleichsweise höherer Einnahme bzw. höheren Blutkonzentrationen antioxidativer Vitamine ein vermindertes Risiko der Kataraktbildung haben. Diese positiven Befunde rechtfertigen die Durchführung breit angelegter Interventionsstudien mit antioxidativen Vitaminen am Menschen.
    Notes: Summary The ocular lens, which is continually exposed to light and ambient oxygen, is at high risk of photooxidative damage resulting in cataract. Oxygen free radicals appear to impair not only lens crystallins which will aggregate and precipitate forming opacities but also proteolytic enzymes whose function it would be to eliminate the damaged proteins. Apart from an enzymatic defense system consisting of superoxide dismutase, catalase and glutathione peroxidase against excited oxygen species the lens contains the antioxidant vitamins C, E and presumably β-carotene as another line of defense. In vitro and in vivo studies in different animal species have demonstrated a significant protective effect of vitamins C and E against light-induced cataract. Sugar and steroid cataracts were prevented as well. Epidemiological evidence in humans suggests that persons with comparatively higher intakes or blood concentrations of antioxidant vitamins are at a reduced risk of cataract development. These positive findings established by several research groups justify extensive intervention trials with antioxidant vitamins in humans using presenile cataract development as a model.
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  • 59
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    European journal of nutrition 28 (1989), S. 103-129 
    ISSN: 1436-6215
    Keywords: geeignete Spezies ; analoge Reaktionen ; optimale Standardisierung ; ethische Grundsätze ; spezifische Alternativen ; suitablespecies ; analogousreactions ; optimalstandardization ; ethicalprinciples ; specificalternatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Experimental animals may serve as models for human beings, if analogies between animal and human functions exist. Therefore, the selection of species and strain plays an important role in the development of such models. Knowledge of the nutritional and physiological characteristics of a species is a prerequisite for the composition of complete diets. Often, preliminary work has to begin at the breeding farm in order to make use of such curative models possible. Only when the high requirements of standardization of experimental animals are met can clinical and subclinical symptoms be determined distinctly. By means of sensitive biochemical reactions, imbalances and interactions of nutritive and active ingredients, as well as successful substitutions, can be recorded. The study of absorption and metabolism of preparations is made possible by observing these reactions. However, negative influence on the results of analysis must be eliminated by correct selection of narcotics, and the proper excision and storage of organs. Because of its importance for the planning and evaluation of experiments, biometry is an integral part of every research project. The scientific information which must be gained from the whole experimental animal cannot be substituted by either isolated organs and cell cultures, or by means of computer simulation. A demanding effort, which includes biotechnological methods, is necessary to further reduce the number of experimental animals and, simultaneously, to enhance experimental evidence. In any case, all scientific aims must be in accordance with the ethical principles of the Society for the Prevention of Cruelty to Animals.
    Notes: Zusammenfassung Versuchstiere können als Modell für den Menschen herangezogen werden, wenn Analogien zwischen tierischen und menschlichen Funktionen bestehen. Deshalb kommt der Wahl von Spezies und Stamm bei der Entwicklung solcher Modelle eine besondere Bedeutung zu. Die Kenntnis der ernährungsphysiologischen Besonderheiten einer Spezies ist die Voraussetzung für die Herstellung vollwertiger Diäten. Oft sind Vorleistungen bereits in der Zuchtstation zu erbringen, um kurative Modelle überhaupt erst anwenden zu können. Nur wenn die hohen Anforderungen an die Standardisierung des tierexperimentellen Teiles einer Untersuchung erfüllt sind, können klinische und subklinische Symptome eindeutig zugeordnet werden. Mit empfindlichen biochemischen Reaktionen lassen sich sowohl Imbalanzen und Interaktionen von Nähr- und Wirkstoffen sowie Substitutionserfolge erfassen. Ebenso können die Resorption und der Stoffwechsel von Präparaten verfolgt werden. Negative Auswirkungen auf das Analysenergebnis sind bereits bei der Wahl des Narkotikums, der Entnahme und Lagerung von Organproben auszuschließen. Wegen ihrer Bedeutung für die Planung und Auswertung stellt auch die Biometrie einen integrierenden Bestandteil eines jeden Forschungsprojektes dar. Die am Ganztier erzielte wissenschaftliche Information kann weder mit isolierten Organen und Zellkulturen, noch mit Hilfe simulierender Computersysteme erreicht werden. Große Anstrengungen, auch unter Einbeziehung biotechnologischer Methoden, sind erforderlich, um die Tierzahlen weiterhin zu reduzieren und gleichzeitig die Aussagekraft der Experimente zu erhöhen. In jedem Fall jedoch müssen die wissenschaftlichen Zielsetzungen mit den ethischen Anliegen des Tierschutzes in Einklang gebracht werden.
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  • 60
    ISSN: 1436-6215
    Keywords: Fettemulsion ; MCT ; LCT ; Lipoproteine ; Elimination ; fat emulsion ; triglycerides ; medium chain (MCT) triglycerides ; long chain (LCT) ; lipoprotein elimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary A fat emulsion containing 20% fat as medium chain triglycerides (MCT) and long chain triglycerides (LCT) (1∶1) was injected as a bolus in an amount of 0.2 g fat per kg body weight to six patients, three to five days after a serious injury. Triglyceride concentrations increased within two min in lipoprotein fractions d〈0.95 g/ml (Chylomicrons), d〈1.006 g/ml (VLDL), d〈1.063 g/ml (LDL) and d〈1.21 g/ml (HDL). Sixty minutes after injection triglyceride concentrations had again reached preexperimental values in all lipoprotein fractions. Cholesterol values did not change. According to the composition of the fat emulsion, linoleic acid content increased in triglycerides of all lipoprotein fractions, whereas octanoic and decanoic acid did so only in triglyceride-rich lipoproteins (d〈1.006). Half-life values of elimination of octanoic acid (3.3 min) and decanoic acid (3.9 min) in triglycerides of lipoprotein fraction d〈1.006 were nearly half as short as that of long chain fatty acids (linoleic acid, 6.4 min; oleic acid, 6.5 min; palmitic acid, 7.5 min). Thus in contrast to LCT, MCT are only found in triglyceride-rich lipoproteins (d〈1.006) and are also eliminated more rapidly.
    Notes: Zusammenfassung Eine Fettemulsion mit 20% Fett aus mittelkettigen Triglyceriden (MCT) und langkettigen Triglyceriden (LCT) (1∶1) wurde als Bolus in einer Menge von 0,2 g Fett pro kg Körpergewicht bei 6 Patienten 3–5 Tage nach einem schweren Unfall injiziert. Die Triglyceridkonzentrationen stiegen innerhalb von 2 Minuten in den Lipoproteinfraktionen d〈0,95 g/ml (Chylomikronen), d〈1,006 g/ ml (VLDL), d〈1,063 g/ml (LDL) und d〈1,21 g/ml (HDL) an. 60 Minuten nach der Injektion hatten die Triglyceridkonzentrationen bereits wieder in allen Lipoproteinfraktionen dsie Vorwerte erreicht. Die Cholesterinkonzentrationen änderten sich nicht. Entsprechend der Zusammensetzung der Fettemulsion nahm der Linolsäureanteil in den Triglyceriden aller Lipoproteinfraktionen zu, während Octansäure und Decansäure nur in den triglyceridreichen Lipoproteinen (d〈1,006) anstiegen. Die Halbwertszeiten der Elimination der Octansäure (3,3 min) und Decansäure (3,9 min) in den Triglyceriden der Lipoproteinfraktion d〈1,006 waren fast halb so kurz wie die der langkettigen Fettsäuren (Linolsäure, 6,4 min; Ölsäure, 6,5 min; Palmitinsäure, 7,5 min). Demzufolge findet man im Gegensatz zu den LCT die MCT nur in den triglyceridreichen Lipoproteinen (d〈1,006), und sie werden daraus auch rascher eliminiert.
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  • 61
    ISSN: 1436-6215
    Keywords: RBP secretion ; vitamin A ; carotenoids ; goat ; RBP-Sekretion ; Vitamin A ; Carotinoide ; Ziege
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Die Sekretion von retinolbindendem Protein (RBP) ins Blut von Ziegen wurde nach unterschiedlicher Verabreichung von Vitamin A (Retinylacetat), Β-Carotin und einer Mischung von pflanzlichen Carotinoiden untersucht. Die Zufuhr dieser Verbindungen — entweder in einer einzelnen hohen Dosis oder in der gleichen Menge gleichmäßig auf 4 Tagesdosen verteilt — führte zu einem deutlichen Anstieg der RBP-Sekretion. Diese Sekretion erfolgte in zwei Phasen, von denen eine früh und eine später einsetzte.
    Notes: Summary Studies were conducted on the secretion of retinol-binding protein (RBP) in blood of goats given different treatments of preformed vitamin A, Β-carotene and plant carotenoids. Administration of these sources either in a single massive dose or massive dose split into four equal doses, markedly increased the secretion of RBP in blood. The secretion of RBP in blood occurred at least in two phases, one at early periods and the other at later periods.
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  • 62
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    European journal of nutrition 28 (1989), S. 265-265 
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    Source: Springer Online Journal Archives 1860-2000
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  • 63
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    European journal of nutrition 28 (1989), S. 300-309 
    ISSN: 1436-6215
    Keywords: tobacco protein ; Arnes test ; micronucleus test ; chromosome aberration test ; SCE test ; Tabakprotein ; Ames-Test ; Mikrokerntest ; Chromosomenaberrationstest ; Schwesterstrangaustauschtest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Tabakprotein wurde im Ames-Test und in drei In-vivo-Tests auf Mutagenität geprüft. In den Salmonellenstämmen TA 98 und TA 100 bewirkten Methanolextrakte des Tabakproteins und der Urin von Ratten, die mit Tabakprotein gefüttert waren, erhöhte Revertantenzahlen, nicht aber Fäzesextrakte. Im Mikrokerntest wurden durchgehend schwach positive Effekte des Tabakproteins in Chinesischen Hamstern und zwei Mäuse-Inzuchtstämmen erzielt, desgleichen im Chromosomenaberrationstest und im SCE-Test an Chinesischen Hamstern. Die in den analytischen Angaben des Tabakproteins ausgewiesenen Substanzen Nikotin, Chlorogensäure und Rutin wurden einzeln im Chromosomenaberrationstest geprüft. Dabei wurde das Nikotin als der verantwortliche Faktor oder einer der verantwortlichen Faktoren für die schwach positiven Testergebnisse erkannt.
    Notes: Summary Tobacco protein was assayed for mutagenicity using the Ames test and three in vivo tests. In the Salmonella strains TA 98 and TA 100 methanolic extracts of the tobacco protein and urine of rats fed tobacco protein exhibited increased revertant numbers, but extracts of feces did not. Using the micronucleus test throughout, weak mutagenic effects after feeding the tobacco protein were detected in Chinese hamsters and two inbred strains of mice, and again in Chinese hamsters when the chromosome aberration test and the SCE test were applied. The analytical specifications of the tobacco protein listed nicotine, chlorogenic acid and rutin as components. These were examined separately in a chromosome aberration test, and nicotine was discovered to be the factor or a factor responsible for the weak positive test results.
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  • 64
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    European journal of nutrition 28 (1989), S. 319-326 
    ISSN: 1436-6215
    Keywords: Tryptophanschädigung ; Tryptophangehalt im Brot ; Tryptophangehalt im Keks ; tryptophan degradation ; tryptophan content inbread ; tryptophan content incookie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The change of tryptophan contents in proteins of bread and cookies under technological processing conditions were investigated. Tryptophan contents in all cookie samples were noted to be significantly (p=0.05) reduced in relation to corresponding dough. The relative decreases are significantly correlated with fat content and the degree of unsaturation of fats in the dough of cookies (r=0.802 and r=0.777, p=0.01), independently of various physical parameters during different cookie samples' processing. Tryptophan decrease in proteins of bread during baking was not significant.
    Notes: Zusammenfassung Es wurde der Einfluß des technologischen Herstellungsprozesses auf die Gehaltsveränderung von Tryptophan in Brot- und Kekseiweißen untersucht. Es wurde eine signifikante (p=0,05) Tryptophanabnahme bei dem Backen der verschiedenen Keksarten festgestellt. Diese Abnahme korreliert linear mit dem Lipidgehalt und dem Gehalt an ungesättigten Fettsäuren der entsprechenden Keksteige (r=0,802 bzw. r=0,777 bei p=0,01), unabhängig von bedeutenden Schwankungen der physikalischen Parameter im Produktionsprozeß der verschiedenen Keksarten. Bei Brot blieb der Tryptophangehalt während des Backens praktisch unverändert.
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    European journal of nutrition 28 (1989), S. 345-345 
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  • 66
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    European journal of nutrition 28 (1989), S. 263-264 
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  • 67
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    European journal of nutrition 28 (1989), S. 240-259 
    ISSN: 1436-6215
    Keywords: Data analysis ; health status ; food pattern ; nutrition behavior ; research strategy ; Gesundheitsstatus ; Ernährungsverhalten ; Forschungsstrategie ; Datenanalyse ; Nahrungsmuster ; Ernährungsepidemiologie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Die Beziehungen zwischen Ernährungsfaktoren und Gesundheit in epidemiologischen Studien sind oft inkonsistent. Eine der möglichen Ursachen für diese Gegebenheit kann darin liegen, daß die Vielzahl der potentiellen Ernährungsdimensionen in der spezifischen Untersuchungssituation in unzulänglichem Maße berücksichtigt wurden. Solche Dimensionsbereiche sind vor allem die physiologische Individualität von Menschen, deren verschiedenen Lebensumwelten und die zahlreichen Interdependenzen zwischen Ernährungsvariablen. Diese Tatsachen sollten in ernährungsepidemiologischen Studien dadurch besser erfaßt werden, daß sowohl die Studienmodelle als auch die Datenanalysen entsprechend ausgerichtet werden. Anstelle von Untersuchungen an heterogenen Stichproben ist es ratsam, sich auf spezifische „Typen“ zu konzentrieren, wobei gemäß dem jeweiligen Studienziel solche „Typen“ aus den physiologischen Besonderheiten (z. B. Cholesterinempfindlichkeit), aus biologisch-konstitutionellen Faktoren (z. B. Körperbau-Typus), aus Lebensstil-Eigenschaften oder anderen Faktorenbereichen abgeleitet werden können. Die Variationsbreite der Ernährungsfaktoren geht über die üblicherweise verwendeten Nährstoffdimensionen hinaus, doch sie läßt sich durch integrierte Indizes — die „Nahrungsmuster“ — zusammenfassen. Zu solchen „Nahrungsmustern“ kann man auf verschiedene Weise kommen. Sie können aus Theorien abgeleitet werden und zu bestimmten Bewertungsrastern führen. Man kann sie aber auch mittels multivariater statistischer Analyse suchen. Die prinzipiellen Wege, die zu „Nahrungsmustern“ führen, werden dargestellt und diskutiert. Die hier vorgestellten und diskutierten Ideen leiten zu einem verbesserten Untersuchungsmodell für ernährungsepidemiologische Studien über. Die „Nahrungsmuster“ nehmen dabei eine wichtige Position ein. Man kann annehmen, daß durch diesen Untersuchungsansatz neue Erkenntnisse über die Beziehungen zwischen Ernährung und Gesundheit sichtbar werden, die helfen, den bisher noch diffusen Untersuchungsbereichen klare Konturen zu geben.
    Notes: Summary The relation between nutritional factors and health investigated in epidemiological studies are often inconsistent. One of the reasons for such findings can be the improper addressing of the multitude of nutritional dimensions in the specific study situation such as physiological individuality of human beings, different living conditions, or numerous interdependencies between nutritional variables. Epidemiological research in nutrition and health should recognize such facts and work with appropiate study models and adequate data analyses. Instead of investigating heterogenous populations it is advisable to concentrate on specific “types” of people. Under consideration of the study goals such “types” can be compiled according to physiological properties, e.g., cholesterol sensitivity, or biological-constitutional factors such as body build, life-style entities, or other factors. The variety of nutrition factors far beyond the commonly applied nutrient values can be expressed in integrated indices of “food patterns”. Such “food patterns” can be derived in many ways. They can be deduced from theories by using specific criteria, but also explored by modern multivariate statistical analyses. The ways leading to “food patterns” are discussed. The ideas presented and discussed in this paper lead to an improved model for research in the field of nutrition and health with integrated indices of “food patterns” as the critical point. It is assumed that using this approach will generate new insight in the relation of nutrition and health, a currently still diffuse research area.
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  • 68
    ISSN: 1436-6215
    Keywords: dietarycalcium ; dietarymagnesium ; dietaryphosphorus ; serumcholesterol ; livercholesterol ; fecalbile acids ; rats ; Calcium ; Magnesium ; Phosphor ; Serumcholesterin ; Lebercholesterin ; Gallensäure im Kot ; Ratten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Während 28 Tagen wurden an Ratten cholesterinfreie halbgereinigte Diäten, die verschiedene Konzentrationen von Calcium (0,13–0,75 g/100 g), Magnesium (0,02–0,04 g/100 g) oder Phosphor (0,2–0,8 g/100 g) enthielten, verabreicht. Die unterschiedlichen Mineralkonzentrationen hatten keinen Einfluß auf Serum- und Lebercholesterin oder die Ausscheidung von Gallensäuren im Kot.
    Notes: Summary Female rats were fed cholesterol-free, purified diets with different concentrations of calcium (0.13–0.75%, w/w), magnesium (0.02 or 0.04%) or phosphorus (0.2–0.8 %) as the only dietary variable. After 28 days, no effects of the minerals were found on liver cholesterol concentrations and rates of fecal excretion of bile acids.
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  • 69
    ISSN: 1436-6215
    Keywords: Meerschweinchen ; Ascorbinsäure ; Adaption im Stoffwechsel ; Vitamin C ; guinea pigs ; adaptation inascorbic acid metabolism ; blood ; liver ; spleen ; adrenal glands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Eine Substitution mit 5 mg/100 g Vitamin C im Futter stellt für Meerschweinchen anscheinend die untere Grenze dar, um ein Überleben zu ermöglichen. Sie scheinen keine Möglichkeit zu haben, den Stoffwechsel der Ascorbinsäure an diese niedrige Substitution zu adaptieren. Meerschweinchen, die über lange Zeit (6–8 Wochen) 20 mg/100 g oder 680 mg/100 g Vitamin C im Futter erhalten hatten, zeigen, nachdem der Vitamin-C-Gehalt im Futter drastisch verändert worden ist, bei den Ascorbinsäurespiegeln mehrerer Organe das für eine ablaufende Adaptation typische Überbzw. Unterschwingen.
    Notes: Summary Guinea pigs supplied with 5 mg/100 g vitamin in the food seem to be at the marginal substitution for a survival and show no ability to adapt the ascorbic acid metabolism to this low support. Guinea pigs longlastingly (6–8 weeks) supplied with 20 mg/100g or 680 mg/100g vitamin C in the food show the typical symptoms of an evolving adaptation by an overshooting in the course of the ascorbic acid levels in several organs after a switch to an extremely different vitamin C supply.
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  • 70
    ISSN: 1436-6215
    Keywords: germ-free rat ; intestinal tract colonization ; interaction ; protection ; Bifidobacterium ; E. coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Das Vorherrschen von Bifidobakterien in normalen, mit Muttermilch ernährten Säuglingen ist gut dokumentiert. Sogar unter ungünstigen hygienischen Bedingungen bei der Geburt und während der Stillperiode entwickeln sich die Bifidobakterien und kolonisieren den Darmtrakt in großer Anzahl. In dieser Studie untersuchten wir die Wechselwirkung zwischen Bifidusbacterium longum und einem pathogenen E.-coli-Stamm in der keimfreien Ratte. Die Proliferation der beiden Bakterienstämme wurde durch sequenzielle Probeentnahme verfolgt. Die licht-mikroskopische und elektronen-mikroskopische Untersuchung von ausgewählten Darmabschnitten zeigte geringgradige morphologische Veränderungen. Die Bifidobakterien schützen die Ratten vollständig gegen Mortalität durch nachfolgende Infektion durch E. coli. Wiederholte Verabreichung von Bifidobakterien verminderte darüber hinaus die E.-coli-Population und hielt diese auf einem niedrigen Niveau. Daher erscheint die keimfreie Ratte als ein angemessenes Modell zum Studium der Entwicklung und Wechselwirkung von beiden Bakterienstämmen. Die Sequenz der Inokulation ist dabei von großer Bedeutung.
    Notes: Summary The predominance of Bifidobacteria in normal breast-fed babies is well established. Even under unfavourable hygienic conditions of delivery and during the breast-feeding period Bifidobacteria develop and colonize the intestinal tract at high concentrations. In the present study we investigated the interaction between Bifidobacterium longum and a pathogenic E. coli strain in the germ-free rat. Sequential counts of the two bacterial strains allowed their proliferation to be followed. Electron microscopic as well as light microscopic examinations of selected intestinal mucosa segments revealed minor morphological changes. Bifidobacterium completely protected the rats against mortality from a consecutive infection with E. coli. Repeated inoculation of Bifidobacteria even decreased and kept down the initial E. coli population. Thus it appears that the germ-free rat is an appropriate model to study the development and interaction of both bacterial species and that the sequence of inoculation is most important.
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  • 71
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    European journal of nutrition 23 (1984), S. 74-74 
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    European journal of nutrition 23 (1984), S. 75-75 
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  • 73
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    European journal of nutrition 13 (1974), S. 72-72 
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  • 74
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    European journal of nutrition 13 (1974), S. 73-80 
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    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The protein quality of some species of freshwater algae was determined by balance-tests with rats (table 1). The microalgal substances used for feeding had been subjected to short-term high-temperature treatment on a roller dryer, which causes an optimal cell disruption and consequently, a good protein digestibility. The green algaScenedesmus obliquus proved to be a plant protein source of best quality, as revealed by a PER of 3.21, a NPU of 68 and a BV of 81 (table 3). — Protein fromCoelastrum proboscideum is of good quality with values of 3.18 (PER), 59 (NPU) and 75 (BV). Depending on the culture medium abnormally high concentrations of non-essential N can be obtained in this alga, which influences negatively the value of the crude protein fraction in the rat-test. — The crude protein of the green algaUronema sp., the largest species of the above test series, is of comparably low quality, with protein values of 2.47 (PER), 45 (NPU) and 55 (BV).
    Notes: Zusammenfassung Die Proteinqualität einiger Algenarten des Süßwassers wurde in Ratten-Bilanzversuchen bestimmt (Tab. 1). Die verfütterten Mikroalgen-Substanzen waren mittels Feinschicht-Walzentrocknung kurzzeitig hocherhitzt worden, was einen optimalen Zellaufschluß und eine gute Eiweißverdaulichkeit bewirkt. Die GrünalgeScenedesmus obliquus erwies sich als eine pflanzliche Proteinquelle bester Qualität, gekennzeichnet durch eine PER von 3,21, eine NPU von 68 und eine BV von 81 (Tab. 3). — Von guter Qualität ist das Eiweiß der GrünalgeCoelastrum proboscideum, mit Werten von 3,18 (PER), 59 (NPU) bzw. 75 (BV). In Abhängigkeit vom Kulturmedium kann es bei dieser Alge zu abnorm hoher Konzentration von nichtessentiellem N kommen, was den Wert der Rohprotein-Fraktion im Rattentest negativ beeinflußt. — Das Roheiweiß der GrünalgeUronema sp., der größten Algenspezies der vorliegenden Testreihen, ist mit Proteinkennzahlen von 2,47 (PER), 45 (NPU) und 55 (BV) von vergleichsweise mäßiger Qualität.
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  • 75
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    European journal of nutrition 23 (1984), S. 143-150 
    ISSN: 1436-6215
    Keywords: rice starch ; cooking quality ; physicochemical properties ; storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung 12 verschiedene Sorten von Reis aus Indien wurden im Hinblick auf die Auswirkung einer Lagerung bei verschiedenen Umgebungsbedingungen für die Dauer von 180 Tagen auf physikalisch-chemische Eigenschaften des Stärkeanteils und auf die notwendigen Garzeiten sowie die beim Garen eintretenden Verluste an Trokkensubstanz bzw. die Wasseraufnahme untersucht. Die Viskosität der Stärke nahm bei allen Reissorten während der Lagerung zu. Bezüglich der Garungszeiten waren keine größeren Unterschiede zwischen den Sorten zu beobachten, während die Wasseraufnahmen sehr unterschiedlich sein konnten.
    Notes: Summary Twelve different varieties of rice including two flavorful varieties grown in the State of Maharashtra (India) have been studied with respect to the effect of storage, at ambient conditions, for 180 days on the physicochemical properties of the starch component as well as cooking quality.
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  • 76
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    European journal of nutrition 23 (1984), S. 157-170 
    ISSN: 1436-6215
    Keywords: Dünndarmresorption ; Tumorstadium ; histologischer Typ ; Organlokalisation ; Ernährungstherapie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary A deteriorating nutritional condition combined with the loss of body mass is one of the most noticeable symptoms of cancer. In order to answer the question to what extent absorption disorders in the small intestine are responsible for the malnutrition which develops, we studied 54 oncologic patients using modified D-xylose absorption tests. After the oral and intravenous administration of 10 g D-xylose at different times, the degree of absorption dependent on time was determined on the basis of a biophysical model. When accompanied by general nonspecific tumor symptoms, the frequency of absorption restrictions was increased. An established relation was found between the extent of small intestinal absorption, on the one hand, and the degree of spreading (staging) and the degree of histological differentiation of the carcinoma, on the other hand. Enteral absorption disorders represent a partial cause for the development of malnutrition in cancer patients and must be viewed as a result of a metabolic situation which is distinctly catabolic.
    Notes: Zusammenfassung Die Verschlechterung des Ernährungszustandes und die Abnahme an Körpermasse ist eines der auffälligsten Symptome des Krebsleidens. Zur Beantwortung der Frage, inwieweit Störungen der Dünndarmresorption für die sich entwickelnde Malnutrition verantwortlich zu machen sind, untersuchten wir 54 onkologische Patienten mit Hilfe des modifizierten D-Xylose-Resorptionstests. Nach zeitlich getrennter oraler und intravenöser Applikation von 10 g D-Xylose wurde auf der Grundlage einer biophysikalischen Modellierung das Ausmaß der Resorption in Abhängigkeit von der Zeit ermittelt. Mit dem Auftreten allgemeiner unspezifischer Tumorsymptome erhöht sich die Häufigkeit von Resorptionseinschränkungen. Es konnte eine gesicherte Beziehung zwischen Ausbreitungsgrad (Staging), histologischem Differenzierungsgrad des Karzinoms einerseits und dem Ausmaß der Dünndarmresorption andererseits nachgewiesen werden. Enterale Resorptionsstörungen stellen eine Teilursache bei der Entwicklung einer Mangelernährung bei Krebskranken dar und sind selbst als Folge der katabol akzentuierten Stoffwechsellage anzusehen.
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  • 77
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    European journal of nutrition 23 (1984), S. 219-229 
    ISSN: 1436-6215
    Keywords: Maillard-Produkte ; Protein-Qualität ; Wachstum und Nephrozytomegalie bei Ratten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Two experiments lasting 10 vs. 8 weeks respectively were conducted in order to determine the effects of feeding heated proteins (casein + glucose, 65 °C for 4 days) on histopathological alterations in the kidneys of rats. Histological examinations showed enlarged epithelial cells and cell nuclei in the pars recta of the proximal tubules (inner stripe of the inner kidney cortex zone) beginning after two weeks of the test. The differences in the average size of the nuclei were significant after a feeding period of 6, 8 or 10 weeks (44 rats in the testgroup vs. 36 rats in the controlgroup respectively). In this way the alterations are very similar to that which are induced by lysinoalanine. The substance causing the lesions seems not to be fructoselysine although it was present in the heat damaged diets in concentrations of 16,000–23,000 ppm but is suggested among the substances formed in the early and advanced stage of Maillard reaction.
    Notes: Zusammenfassung In zwei Versuchen von 10 bzw. 8 Wochen Dauer wurde der Einfluß der Fütterung eines hitzegeschädigten, lysinoalaninfreien Proteins (Casein+Glucose, 65 °C, 4 Tage) auf histopathologische Veränderungen in Rattennieren untersucht. In den histopathologischen Präparaten der Nieren waren schon nach zwei Wochen vergrößerte Tubulusepithelzellen in der pars recta des proximalen Tubulus zu erkennen. Zellkernmessungen in der 6., 8. und 10. Versuchswoche ergaben eine signifikante Erhöhung der mittleren Zellkerngröße bei den Versuchstieren (n=44) im Vergleich zu den Kontrolltieren (n=36). Damit waren die Veränderungen vergleichbar mit den aus der Literatur bekannten, durch Lysinoalanin induzierten histopathologischen Befunden. Das auslösende Agens für die Nephrocytomegalie konnte nicht identifiziert werden. Es dürfte im Bereich der vielen Zwischenprodukte aus dem Initial- und Zwischenstadium der Maillard-Reaktion zu suchen sein.
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  • 78
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    European journal of nutrition 23 (1984), S. 113-125 
    ISSN: 1436-6215
    Keywords: Muttermilch ; Triglyceride ; Cholesterin ; Phospholipide ; Fettsäuren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The dependency of the human milk lipids triglycerides (TG), cholesterol (Chol), phospholipids (PL), and the fatty acid composition of both TG and PL on the stage of lactation, the day time, the course of one nursing, and on the mother's diet was shown by analyzing more than 350 milk samples. In progressing lactation (day 1–36) TG increased from 1.9 to 3.9 g/100 ml. Chol decreased from 35 to 20 mg/100 ml, while PL remained with 40 mg/100 ml rather constant. The resulting decrease in the PL/TG- and Chol/TG-ratios indicates an increasing size of fat globules. During one nursing, TG and Chol increased four-fold and PL doubled. During the day lipids (TG, Chol, PL) of the milks of German mothers reached their peak values in the afternoon, whereas the ones of milks obtained from English mothers were found in the late evening. With 3 mothers (n=3) carbohydrate-rich diets (65 cal%) led to an increase of the milk fat content. In contrast high fat diets (50 cal%) resulted in milks with low fat contents (2.6/4.1 g/100 ml). Chol (20.8/37.5 mg/100 ml) and PL (26.4/ 45.3 mg/100 ml) showed the same dependency. The fatty acid composition changed mainly dependent on the duration of the lactation and the mother's diet, but not during one single nursing and during the day. Long-chain polyunsaturated fatty acids were higher in early than in mature milks. The P/S-ratio of TG was found to remain constant in progressing lactation (0.32), whereas the one of PL increased from 0.6 to 0.71. Dependent on the regimen of the mother the P/S-ratios (n=3) were found to be 0.27 (high carbohydrate diet) and 0.46 (high fat/high linoleic acid diet).
    Notes: Zusammenfassung Mit der Analyse von über 350 Frauenmilchproben konnte die Abhängigkeit der Frauenmilchlipide — Triglyceride (TG), Cholesterin (Chol), Phospholipide (PL), Fettsäurezusammensetzung der TG und PL — vom Stadium der Laktation, der Tageszeit, dem Verlauf des Stillvorgangs und der mütterlichen Ernährung gezeigt werden. Im Verlauf der Laktation (1.–36. Tag) nahm der TG-Gehalt der untersuchten Proben von 1,9 auf 3,9 g/100 ml zu. Chol sank von 35 auf 20 mg/100 ml. Der PL-Gehalt blieb mit 40 mg/100 ml weitgehend konstant. Die sich aus den Ergebnissen ableitenden Chol/TG- und PL/TG-Quotienten deuten auf eine Zunahme der Fettkügelchengröße im Laufe der Laktation hin. Während eines Stillvorganges stiegen die TG und Chol um das nahezu Vierfache an, wogegen sich der PL-Gehalt nur verdoppelte. Über den Tag gesehen hatten Milchen deutscher Mütter ihre höchsten Lipidwerte (TG, Chol, PL) am Nachmittag, wogegen die Maxima der englischen Milchproben am späten Abend gefunden wurden. Kohlenhydratreiche Ernährungsweise (65 cal%) führte bei Müttern (n=3) zu einem Anstieg des Milchfettgehalts, wogegen umgekehrt aus fettreicher Ernährung relativ fettarme Milchen resultierten (2,6/4,1 g/100 ml). Entsprechend verhielten sich Chol (20,8/37,5 mg/ 100 ml) und die PL (26,4/45,3 mg/100 ml). Die Fettsäurezusammensetzung änderte sich vor allem in Abhängigkeit von der Laktation und der mütterlichen Ernährung, nicht aber während des Stillvorganges und im tageszeitlichen Verlauf. Frühe Milch ist besonders reich an langkettig polyungesättigten Fettsäuren. Der P/S-Quotient der TG blieb im Verlauf der Laktation mit 0,32 weitgehend konstant, wogegen derjenige der PL von 0,6 auf 0,71 anstieg. Je nach Ernährungsweise der Mutter lag der P/S-Quotient (n=3) bei 0,27 (kohlenhydratreich) und bei 0,46 (fettreich, linolsäurereich).
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  • 79
    ISSN: 1436-6215
    Keywords: tierexperimenteller Thiaminmangel ; Pyruvatverwertungsstörung ; Laktazidose ; Aminosäurehomöostasestörung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The metabolic effects of thiamine deficiency on the metabolism of lactate, acid-base and amino acids were evaluated both with and without muscular exercise in a controlled animal experiment. Thiamine deficiency — in good correlation with its biologic halftime and also with reports in the literature — caused with a latency of 17–56 days and statistically proven in parallel the following effects: 1. hyperlactemia 2. in comparison to controls elevated plasma concentrations of threonine, alanine, glutamic acid, glutamine, histidine, methionine, taurine, valine, isoleucine and leucine 3. distinctly lowered — also in comparison to controls — plasma concentrations of arginine and tryptophan. Furthermore, in the thiamine deficient rats predetermined muscular exercise caused the following effects, also occurring concomitantly: 1. life threatening lactacidosis 2. marked — statistically prover — disturbance of the homeostasis of alanine, glycine, methionine and arginine 3. distinctly — and statistically significant — elevated plasma concentration of proline 4. statistically significantly lowered plasma concentrations of taurine. The reasons for all these changes under thiamine deficiency is most likely a metabolic one primarily due to a disturbance of the utilization of pyruvate in the liver. An additional disturbance of both the transsulfuration pathway and the Krebs-Henseleit-cycle seems probable. The physiologic regulation of pyruvate, lactate, alanine, serine and tyrosine during muscular exercise in correlation to the controls is being discussed.
    Notes: Zusammenfassung In einer kontrollierten tierexperimentellen Studie werden die metabolischen Wirkungen eines definierten Thiaminmangels auf den Lactat-, Säuren-Basen- und Aminosäurenhaushalt unter Ruhe- und Belastungsbedingungen überprüft. In guter Korrelation zur biologischen Halbwertszeit von Thiamin und in Übereinstimmung mit Literaturangaben bedingte die Thiaminmangelkost mit einer Latenz von 17 bis 56 Tagen parallel 1. statistisch gesicherte Hyperlaktatämien, 2. gegenüber den Kontrollwerten sehr deutlich, in der Mehrzahl statistisch gesichert erhöhte Plasmakonzentrationen von Threonin, Alanin, Glutaminsäure, Glutamin, Histidin, Methionin, Taurin, Valin, Isoleucin und Leucin sowie 3. gegenüber den Kontrollwerten sehr deutlich und statistisch gesichert erniedrigte Plasmakonzentrationen von Arginin und Tryptophan. Darüber hinaus bedingte die energetische Belastung im genormten Belastungstest bei den thiaminarm ernährten Tieren parallel bedrohliche Laktazidosen, erheblich, statistisch gesichert ausgeprägtere Homöostasestörungen von Alanin, Glycin, Methionin und Arginin, sehr deutlich und statistisch signifikant erhöhte Plasmakonzentrationen von Prolin sowie statistisch signifikant abgesunkene, wieder im Bereich der Kontrollwerte liegende Plasmakonzentrationen von Taurin. Für die Mehrzahl der unter Thiaminmangel erhobenen Befunde muß als entscheidende metabolische Ursache eine hepatische Verwertungsstörung von Pyruvat angenommen werden. Störungen des Transsulfuration-Pathway und Krebs-Henseleit-Zyklus erscheinen wahrscheinlich. Die physiologische Regulation von Pyruvat, Lactat, Alanin, Serin und Tyrosin bei muskulärer Arbeit wird anhand der Kontrollwerte diskutiert.
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    European journal of nutrition 23 (1984), S. 230-236 
    ISSN: 1436-6215
    Keywords: selenium ; vegetable ; fruit ; cereals ; infant food
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Der Selengehalt der Nahrung weist große regionale Unterschiede auf. Nahrungsproben von Säuglings- und Kleinkinderkost aus Nordrhein-Westfalen wurden mit Hilfe der instrumentellen Neutronenaktivierungsanalyse untersucht und zeigten, daß der Selengehalt der hiesigen Gemüse und Früchte durchschnittlich etwa 5 ng/g beträgt. Nur Proben von Kartoffeln und Bananen weisen einen höheren Selengehalt auf. Bananen, importiert aus verschiedenen Gebieten der Erde, zeigen eine extrem hohe Schwankungsbreite mit Werten zwischen 4 und 164 ng Se/g. Fertiggerichte für Säuglinge und Kleinkinder haben einen der Kuhmilch vergleichbar niedrigen Selengehalt von durchschnittlich 24 ng/g. Außer Eiern enthalten nur Milchbreie und einige andere getreidehaltige Produkte mehr als 100 ng Se/g.
    Notes: Summary The selenium content of food exhibits great regional differences. Food samples of infants and young children from the North Rhine-Westphalia State in the Fed. Rep. of Germany were analysed by instrumental neutron activation analysis and showed that the average selenium content of local vegetable and fruit is below 5 ng/g wet weight. Only samples of potatoes and bananas exhibit higher selenium contents. The bananas, probably imported from different areas of the world, show an extremely high variance with values between 4 and 164 ng Se/g. Commercially available ready-for-use meals for infants or young children have a low Se content (median 24 ng/g) which is comparable to cow's milk. Besides eggs, cereals enriched with milk and some other cereal products contain more than 100 ng Se/g.
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  • 81
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The course of cholesterol-7α-hydroxylase activity was observed for 14 days in 3 groups of rats on 20% fat diets which were of different fatty acid compositions. Determinations were done at 09.00 and 23.00 hr. In the controls the circadian variations of the enzyme were confirmed. Feeding of saturated fatty acids lead to a reduced 7α-hydroxylation of cholesterol in the dark period, whereas feeding of polyunsaturated fatty acids (13% as well as 56% of total fatty acids) showed an enzyme activity unchanged in the dark period but slightly enhanced in the light period.
    Notes: Zusammenfassung Der Verlauf der Cholesterin-7α-Hydroxylase-Aktivität wurde während 14 Tagen an 3 Gruppen fettreich (20%) ernährter Ratten beobachtet (Messungen jeweils um 9.00 und 23.00 Uhr), wobei die einzelnen Diäten sich in der Fettsäurenzusammensetzung unterschieden. Während die Werte der Kontrollgruppe den Circadianrhythmus des untersuchten Enzyms bestätigten, führt Ernährung mit gesättigten Fettsäuren zu einem Absinken der 7α-Hydroxylierung des Cholesterins in der Dunkelphase. Dagegen weisen die Diäten mit polyungesättigten Fettsäuren (13% bzw. 56% des Fettsäurengehaltes) in der Dunkelphase unveränderte, in der Hellphase eher erhöhte Cholesterin-7α-Hydroxylase-Aktivität auf.
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  • 82
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    European journal of nutrition 13 (1974), S. 186-188 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Notes: Zusammenfassung Durch Kombinierung der dritten FormelCowgills und der vonRathbun undPace und eines daraus errechneten Nomogramms ergibt sich ein einfaches Verfahren zur Beurteilung des prozentualen Fettgehaltes des Körpers aus Körpergewicht und Körperhöhe.
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    European journal of nutrition 28 (1989), S. 1-2 
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    Source: Springer Online Journal Archives 1860-2000
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  • 84
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    European journal of nutrition 28 (1989), S. 17-31 
    ISSN: 1436-6215
    Keywords: Calciumversorgung ; Bioverfügbarkeit von Calcium ; Osteoporose ; osteoporosis ; osteoporosis ; pathogenesis of ; calcium supply ; nutritional aspects of osteoporosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Osteoporosis primarily affects women and is a bone disease of great socialmedical and economic importance. The pathogenesis is thought to be of multifactorial origin. The current work discusses nutritional aspects relative to osteoporosis. In this aspect, calcium supply seems to have the largest preventional importance. In contrast, food statistics and individual food records show that calcium intake of females is below the DGE's recommendation of 800 mg/daily. Furthermore, in view of skeletal integrity, calcium intake should be increased up to 1,500 mg/daily after menopause, because of endocrinological changes in calcium metabolism. Therefore, calcium intake on the whole is usually found to not be sufficient. The importance of further nutritional aspects for manifestation of osteoporosis are discussed. In some cases, influences on skeletal integrity are quite evident, whereas the literature has only infrequently drawn conclusions about the pathogenesis of osteoporosis.
    Notes: Zusammenfassung Die Osteoporose ist eine Knochenerkrankung mit großer sozialmedizinischer und volkswirtschaftlicher Bedeutung, von der überwiegend Frauen betroffen sind. Die Pathogenese der Erkrankung ist multifaktoriell. Der derzeitige Kenntnisstand über die Bedeutung alimentärer Faktoren wird in diesem Zusammenhang dargestellt. Größte präventive Bedeutung besteht seitens der Ernährung in einer ausreichenden Calciumversorgung. Verzehrsstatistiken und individuelle Verzehrsprotokolle zeigen dagegen, daß die Versorgung mit Calcium beim weiblichen Geschlecht im Durchschnitt unter der Empfehlung der DGE von 800 mg/d liegt. Hinzu kommt, daß zur Aufrechterhaltung der Knochenhomöostase aufgrund endokriner Umstellungen im Calciumstoffwechsel empfohlen wird, nach der Menopause die Calciumaufnahme auf 1500 mg/d zu steigern. Die Versorgungslage mit Calcium ist somit insgesamt als sehr unzureichend anzusehen. Weitere ernährungsbedingte Faktoren mit Bedeutung für eine Osteoporosemanifestation werden diskutiert. In einigen Fällen ist hierdurch eine Beeinflussung der Knochenhomöostase durchaus denkbar, häufig ist das Datenmaterial allerdings noch zu unvollständig oder fehlt völlig, um Zusammenhänge zur Osteoporoseentstehung herstellen zu können.
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    European journal of nutrition 28 (1989), S. 93-102 
    ISSN: 1436-6215
    Keywords: Vitamin A-Status ; polyhalogenierte aromatischeKohlenwasserstoffe ; Metabolismus ; polareRetinoide ; vitamin A status ; polyhalogenated aromatic hydrocarbons ; metabolism ; polar retinoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Ausreichende Vitamin-A-Speicher und -Gewebslevel werden durch ein Gleichgewicht von Nachfrage der Gewebe nach Vitamin A und der Vitamin-A-Aufnahme durch die Nahrung aufrechterhalten und durch viele Faktoren beeinflußt. Es ist hinreichend bekannt, daß polyhalogenierte aromatische Kohlenwasserstoffe (PHAH) die Vitamin-A-Speicher der Leber erniedrigen. Neuere Untersuchungen deuten darauf hin, daß die Erniedrigung der Vitamin-A-Leberspeicher von einem Anstieg des Vitamin-A-Gehaltes in Serum und Niere sowie von einer erhöhten Abgabe von Vitamin-A-Metaboliten in Urin und Fäzes begleitet sind. Die Untersuchung der Vitamin-A-Verteilung in verschiedenen Geweben zeigte, daß die Zufuhr von PHAH zu einem verstärkten Auftreten polarer Vitamin-A-Metaboliten führt. Es ist wahrscheinlich, daß die PHAH Enzyme beeinflussen, die entscheidend für die Regulation von Vitamin-A-Speichern sowie der Aktivität von für den Vitamin-A-Stoffwechsel wichtigen Enzymen sind.
    Notes: Summary Adequate stores and adequate tissue levels of vitamin A are maintained by a balance of tissue demands and dietary intake of the vitamin and are modified by many factors, including xenobiotics. It is well established that exposure to polyhalogenated aromatic hydrocarbons (PHAH) decreases hepatic content of vitamin A. Recent findings indicate that hepatic depletion of vitamin A is accompanied by an increase in serum and renal vitamin A content and enhanced excretion of vitamin A metabolites in urine and feces. Examination of tissue retinoid profiles reveals that PHAH exposure causes the generation of increased amounts of polar retinoids. It is very likely that PHAH affect enzymes crucial for regulation of vitamin A storage as well as enhance activities of specific enzymes in vitamin A metabolic pathway.
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  • 86
    ISSN: 1436-6215
    Keywords: Stickstoffbilanz ; Proteinbedarf ; Krallenaffen ; Callitrichidae ; nitrogen balance ; protein requirement ; marmoset ; C allitrichidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung An erwachsenen männlichen Weißbüscheläffchen (Callithrix jacchus) erfolgten Stickstoffbilanzmessungen bei Verfütterung von „halbsynthetischen“ und „synthetischen“ Diäten mit Proteingehalten von null bis sieben Prozent. Der tägliche Stickstoffverlust bei längerer proteinfreier Ernährung („Abnutzungsquote“) betrug 131±16 mg/kg Körpergewicht0,75. Eine N-Bilanz =±0 wurde mit der Zufuhr von 261 mg N/kg0,75 in Form hochwertiger Proteine erreicht. Sehr geringe Proteinaufnahme oder das Fehlen von Arginin und Histidin in einer Aminosäurenmischung führte zu Koprophagie. Es wird gefolgert, daß der Proteinbedarf erwachsener Krallenaffen dem des erwachsenen Menschen (auf der Basis des metabolischen Körpergewichts) sehr ähnlich ist. Etwa 6–7% Protein hoher Qualität, auf Trockensubstanz bezogen, genügen zur Vermeidung einer negativen Stickstoffbilanz.
    Notes: Summary Nitrogen balance studies were conducted in adult male marmosets (Callithrix jacchus) using purified and semipurified diets with protein levels between 0% and 7%. Daily nitrogen loss in a state of prolonged protein free nutrition was 131±16 mg/kg body weight0.75. Zero nitrogen balance resulted from mean daily intake of 261 mg nitrogen/kg0.75 when high quality protein sources were used. Very low protein intake or the lack of arginine and histidine in an amino acid mixture induced coprophagy. It is concluded that the protein requirement of adult marmosets is very similar to the protein requirement of adult humans (based on metabolic body weight). About 6–7% high quality protein, based on dry matter, suffice to avoid a negative nitrogen balance in all individuals.
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    European journal of nutrition 28 (1989), S. 211-224 
    ISSN: 1436-6215
    Keywords: Klinische Ernährungstherapie ; Aminosäurenlösungen,Aminogramme ; Key words ; Clinical nutrition ; amino acids, analytical interpretation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The high value of clinical nutrition as an important component in polypragmatic therapy of severely ill patients with increasing age and restricted compensatory capacity is today unquestioned. The areas of interest and applications have reached a high degree of complexity by many differentiations. The metabolic interrelations under pathological conditions influence especially the application of amino acids. By the enormously increased standards of analytical procedures and instruments huge amounts of data have been gathered but methods and tools for interpreting the complex analytical results have not been developed to the same degree. Considering physiological, pathological biochemical, and biophysical alterations, this review discusses application of amino acids, evaluation and interpretation procedures, as well as indications, contraindications, and effective monitoring. The important regulatory function of the liver in connection with the urea production rate, and the physiological pattern and concentrations of amino acids in plasma are both discussed in detail. Also discussed is the therapeutic advantage of determining total concentration and relative composition of the amino acid pool in plasma, especially in severely ill patients.
    Notes: Zusammenfassung Der hohe Stellenwert der klinischen Ernährungstherapie ist in der heutigen polypragmatischen Therapie schwerstkranker und zum Teil sehr alter Patienten mit eingeschränkter Kompensationsfähigkeit unbestritten. Das Einsatzfeld der klinischen Ernährungstherapie hat dadurch eine starke Differenzierung und einen hohen Komplizitätsgrad erreicht. Diese Wechselbeziehung des pathologischen Ablaufs im Stoffwechsel nach äußeren und inneren Belastungen und den einzelnen Substraten betrifft im hohen Maße die Anwendung von Aminosäuren. Durch die enorme Zunahme der analytischen Leistungsfähigkeit ist das Erkenntnismaterial im Bereich der Aminosäurenversorgung zwar stark angewachsen, aber die Interpretation des komplexen Zusammenhangs der Aminosäuren keineswegs in gleicher Weise verbessert worden. In der vorliegenden Arbeit wird auf dem grobskizzierten Hintergrund physiologischer und pathologischer Stoffwechselzusammenhänge der Einsatz von Aminosäuren dergestalt abgehandelt, daß über eine plausible Interpretation der komplexen analytischen Daten die Indikation, Kontraindikation und überwachung der Aminosäurenapplikation verständlich wird. Vor allem die Bedeutung der Regulation durch die Leber, der Zusammenhang mit der Harnstoffproduktionsrate sowie die differente Bedeutung der mikromolaren und relativen Aminosäurenmuster im Plasma werden so abgehandelt, daß sie für die Interpretation auch schwerkranker Zustände herangezogen werden können.
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  • 88
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    European journal of nutrition 28 (1989), S. 279-299 
    ISSN: 1436-6215
    Keywords: Eisenmangel, Epidemiologie ; Eisenresorption ; Eisensubstitution ; Eisenintoxikation ; iron deficiency ; epidemiology ; iron absorption ; iron substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Iron is an essential trace element. In its heme-form as well as in its non heme-form it is a part of enzymes and hemoproteins. For a safe and adequate dietary intake 10–18 mg of iron are recommended daily. Frequently, this quantity is not available: approximately 20 % of the world population is iron-deficient. In this state the enteral transfer capacity for toxic metals, e.g., Cd and Pb, is increased and the adaptation to physical strain as well as the immunological responses are depressed. Alterations of body iron-stores are almost exclusively balanced by adequate adaptation of the enteral iron-transfer capacity. The mechanism of this adaptation process can neither be satisfactorily explained by the “mucosal block hypothesis”, nor by the “mucosal transferrin hypothesis”. When the time-course of iron storage and its relation to intestinal iron transfer was investigated after i.v. iron administration to iron-deficient rats, the results indicated that the process of adaptation is located in the intestinal mucosa. Intestinal iron loading is decreased in iron deficiency, whereas the iron transfer into the organism is increased. Further investigation is necessary to find out by which mechanism the iron manages to bypass existing mucosal storage capacity in this situation. The geographical distribution of iron deficiency is influenced by a variety of local factors. Still, the paramount causes of iron-deficiency are unbalanced iron losses and the lack of bioavailable iron in the diet. The bioavailability of non heme iron is influenced by the composition of the diet. The effect of promotors of iron absorption, such as meat, amino acids, polycarbonic acids and ascorbate is opposed by the influence of inhibitors, such as bran, soyaproducts, vegetables and egg-dishes. Iron losses are mainly due to blood losses. Thus, the wide distribution of hockworm diseases in tropical areas contributes significantly to the endemic iron-deficiency in these regions. A more physiological loss of iron is caused by menstruation and pregnancy. In small infants the iron-demand of the organism is increased by rapid growth, which in turn increases the intestinal iron transfer. An increased iron-demand can be balanced by an iron-supplemented diet or by pharmaceutical iron compounds. Acute intoxications can be caused by an overdose of such preparations. The pathophysiology and symptoms of acute iron intoxication are summarized. Their frequency has markedly decreased since “childproof” packaging has been introduced for iron-preparations. To meet the increased iron demand of young children, commercial infant formulas are frequently fortified with iron, preferentially with heme-iron. Inlight of the wide distribution of iron-deficiency a general iron fortification of staple food items is discussed controversially.
    Notes: Zusammenfassung Eisen zählt zu den essentiellen Spurenelementen. Es ist in seiner Häm- und Non-Häm-Form Bestandteil von Hämproteinen und Enzymen. Für eine ausreichende und sichere Versorgung des Organismus werden 10 mg bis 18 mg Eisen in der täglichen Diät empfohlen. Diese Menge wird erheblich häufiger unterals überschritten: Ca. 20 % der Weltbevölkerung haben einen Eisenmangel, der sich u.a. in einer gesteigerten enteralen Resorptionsfähigkeit für Cd und Pb äußert sowie in einer Einschränkung der physischen Leistungsfähigkeit und der Infektabwehr. Veränderungen im Körpereisenstatus werden fast ausschließlich durch Anpassung der enteralen Eisenresorption bedarfsgerecht ausgeglichen. Der Mechanismus dieser Anpassung kann weder durch „Mucosa-Block-Hypothese“ noch durch die „Mucosal-Transferrin“-Hypothese befriedigend erklärt werden. Kinetische Untersuchungen an eisenarmen Ratten über die Speicherung parenteral gegebenen Eisens und die Auswirkungen dieser Gabe auf die Eisenresorption deuten jedoch auf einen lokalen Adaptationsprozeß in der intestinalen Mukosa. Dabei ist die Beladung der Mukosa mit angebotenem Eisen im Eisenmangel herabgesetzt, der Transfer dagegen erhöht. Wie das Eisen in diesem Zustand an den vorhandenen Speicherkapazitäten der Mukosa vorbeigeleitet wird, bedarf weiterer Klärung. Die geographische Verteilung des Eisenmangels unterliegt einer Reihe lokaler Einflüsse. Hauptursachen des Eisenmangels sind der Mangel an bioverfügbarem Eisen in der Nahrung und unausgeglichene Eisenverluste. Die Bioverfügbarkeit von Non-Häm-Eisen in der Nahrung wird durch die Zusammensetzung der Diät beeinflußt. Resorptionsfördernde Komponenten wie Fleisch, Aminosäuren, Polycarbonsäuren und Ascorbat saldieren sich in der Wirkung mit Hemmstoffen der Eisenresorption wie z.B. Kleie und Sojaprodukte, Gemüse und Eispeisen. Eisenverluste sind in erster Linie Blutverluste, wobei der in den Tropen verbreitete Befall mit Hakenwürmern starken Anteil an dem dort endemischen Eisenmangel hat. Andere, eher physiologische Eisenverluste entstehen durch Menstruation und Schwangerschaft. Während des raschen Wachstums im Säuglingsalter entsteht ebenfalls ein stark erhöhter Eisenbedarf, der zu gesteigertem Eisentransfer führt. Erhöhter Eisenbedarf kann durch medikamentöse Eisensubstitution oder durch adäquate Ernährung gedeckt werden. Eisenmedikamente können jedoch bei nicht bestimmungsgemäßem Gebrauch zu akuten Intoxikationen führen, deren Bild detailliert beschrieben wird. Die Inzidenz solcher Vergiftungen ist seit Einführung „kindersicherer“ Verpackungen für Eisenpräparate stark zurückgegangen. Zur Deckung des durch Wachstum erhöhten Eisenbedarfs wird kommerzielle Säuglingsbeikost häufig mit Eisen fortifiziert, wobei Häm-Eisen die größeren Vorzüge bietet. Eine allgemeine Fortifikation von Grundnahrungsmitteln mit Eisen ist jedoch trotz der weiten Verbreitung des Eisenmangels stark umstritten.
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  • 89
    ISSN: 1436-6215
    Keywords: guinea pigs ; adaptation ofhepatic microsomal proteins andcytochromes P-450 and b5 to differentvitamin C supplies ; Meerschweinchen, mikrosomales Protein ; Cytochrome P-450 und b5 ; Vitamin-C-Substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Meerschweinchen, die über lange Zeit (6–8 Wochen) 680 mg/ 100 g Vitamin C im Futter erhalten hatten und dann 10 Wochen lang substituiert wurden mit 5 mg/100 g, konnten in der Leber nicht die Gehalte an mikrosomalem Cytochrom P-450 aufrechterhalten 20 mg/100 g, zeigten in der Leber das für eine Adaptation typische Unterschwingen bei dem Gehalt an mikrosomalem Cytochrom P-450.
    Notes: Summary Guinea pigs longlastingly (6–8 weeks) supplied with 680 mg/100 g vitamin in the food and for ten weeks thereafter exposed to: 5 mg/100 g: show no ability to maintain the amounts of hepatic microsomal cytochrome P-450 20 mg/100 g: show the typical symptom of an evolving adaptation by an overshoot of the amounts of hepatic microsomal cytochrome P-450.
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  • 90
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    European journal of nutrition 23 (1984), S. 241-254 
    ISSN: 1436-6215
    Keywords: Verzehrsregulation ; Sättigung ; Verzehrsverhalten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Food intake in man and animals occurs as meals. Because changes in daily food intake in laboratory animals are often associated with corresponding changes in meal size, while meal frequency is less affected, food intake appears to be regulated mainly by the satiety mechanism. Meal size is controlled by feedback or satiety signals originating from the stomach, the small intestine, the liver and the fat depots. Both neural and hormonal (Bombesin?, Cholecystokinin?) feedback signals are elicited in the stomach and intestine. The feedback signals of the liver apparently originate from hepatic chemoreceptors, whose activity is modulated by certain metabolites, in particular glucose. The satiety effect of pancreatic glucagon, whose plasma concentration in the portal vein increases during a meal, also appears to be mediated by the hepatic chemoreceptors. Unmyelated nerve endings are probably the morphological substrate of the hepatic chemoreceptors. There is some evidence that circulating fat metabolites (fatty acids, ketone bodies, glycerol), whose plasma levels are related to the size of the fat depots, function as lipostatic feedback signals. The signal transfer from the gastrointestinal chemo- and mechanoreceptors and from the hepatic chemoreceptors to the central nervous system occurs mainly through vagal afferents. The first relay for this afferents is the nucleus tractus solitarii in the medulla oblongata. The next relay is at the pons, from where neurons project to the hypothalamus and other forebrain areas. The hypothalamus has important integrative functions in the control of food intake.
    Notes: Zusammenfassung Die Nahrungsaufnahme erfolgt bei Mensch und Tier in Form von Mahlzeiten. Da sich Änderungen in der täglichen Nahrungsaufnahme beim Versuchstier meist in entsprechenden Veränderungen der Mahlzeitengröße, nicht aber der Mahlzeitenfrequenz manifestieren, scheint die Nahrungsaufnahme hauptsächlich durch den Sättigungsmechanismus reguliert zu werden. Die Mahlzeitengröße wird durch vom Magen, vom Dünndarm, von der Leber und von den Fettdepots ausgehende Feedback- oder Sättigungs-Signale determiniert. Die gastralen und intestinalen Feedback-Signale scheinen dabei nervaler und hormonaler (Bombesin?, Cholezystokinin?) Natur zu sein. Die Feedback-Signale der Leber gehen offensichtlich von hepatischen Chemorezeptoren aus, deren Aktivität durch bestimmte Metabolite, insbesondere Glucose, moduliert wird. Auch die Sättigungswirkung von pankreatischem Glukagon, dessen Plasmakonzentration während der Aufnahme einer Mahlzeit in der Pfortader ansteigt, kommt wahrscheinlich unter Einschaltung der hepatischen Chemorezeptoren zustande, deren morphologisches Substrat marklose Nervenendigungen sein dürften. Als lipostatische Feedback-Signale fungieren wahrscheinlich im Blut zirkulierende Metabolite des Fettstoffwechsels (Fettsäuren, Ketonkörper, Glyzerin), deren Plasmakonzentrationen mit der Größe der Fettdepots korreliert sind. Die nervale Übertragung der von den gastrointestinalen Chemo- und Mechanorezeptoren sowie den hepatischen Chemorezeptoren ausgehenden Feedback-Signale zum Zentralnervensystem erfolgt hauptsächlich über afferente Vagusfasern. Als erste Relais-Station fungiert diesbezüglich der Nucleus tractus solitarii der Medulla oblongata, von dem aus Nervenfasern über eine Relais-Station in der Pons zum Hypothalamus und anderen Vorderhirnregionen projizieren. Dem Hypothalamus scheinen bei der Regulation der Nahrungsaufnahme wichtige integrative Funktionen zuzukommen.
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  • 91
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    European journal of nutrition 23 (1984), S. 291-291 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
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  • 92
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Summary A promising field of laser utilization is the modification of cell behaviour. Acting on cell biological functions it might be possible to use them to improve medical therapies. This possibility suggests investigation of dosimetry guidelines employing different wavelenghts, power and exposure durations for future potential use of lasers in medicine. In this line of investigation, structural organization and viability of fibroblast has been studied, thus observing that the cell number remains unaltered by low density laser irradiation. Nevertheless, results observed in metabolism variation suggest that, with appropiate irradiation-protocol an efficient modality for enhancement or modulation of cell activity can be found, and thereafter used for therapeutical pourposes.
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  • 93
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    Lasers in medical science 4 (1989), S. 95-103 
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Summary Response of rabbit ear blood vessels and defects on the pig skin to Argon and CO2 lasers, (1 to 4 Watts and 0,1 to 0,2 sec pulses), were analysed. Examining lesion depth and its diameter, it was seen, using both lasers, that when power and time exposure increased, lesion depth increased as well, At the same time, when power was increased and the time of irradiation was constant, the diameter of the lesion remained approximately the same. Histologically, by using Argon laser at 1'5 W, there is no evidence of skin lesion but vessel coagulation occurs. On the other hand, more power (3 to 4 W) produces skin destruction. CO2 laser at 1'5 W causes skin destruction while 3 W penetrates deeper, cutting all tissues and producing vessel-wall fragmentation and haemorrhage. In contrast to CO2 laser, which definitly stops blood circulation by cutting the vessels, Argon laser entails many recurrences and vessels re-start circulation; a fact which is not desirable in cutaneous vascular disorders treatment.
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  • 94
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    Lasers in medical science 4 (1989), S. 213-213 
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
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  • 95
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    Lasers in medical science 4 (1989), S. 235-238 
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract Until now there are no national safety regulations for the medical use of lasers in the Netherlands. However, a committee of experts from hospitals, Health Council of the Netherlands, TNO Medical Technology Unit and industry is preparing recommendations. Their work is based on MPE values and special measurements that are performed to assess divergence and intensity distribution of laser beams, as well as optical and thermal properties of materials that are used in the clinical setting. The final intention of the committee is to advise government and hospitals on technical, environmental and administrative measures for the safety of the various medical laser applications.
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  • 96
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract Accident prevention regulations, e.g. VBG 93 “Laser Radiation”, are a national particularity in the FRG. The regulations issued by the injuries insurance institutes are binding upon employers and employees. The apparatus and equipment safety law makes the VBG 93 obligatory also for manufacturers and importers. National and international standards are referred to in relevant context in the VBG 93. The VBG 93 is also used by the governmental authorities for the protection of labour. The medical apparatus decree has to be observed also for medical application. Some important aspects of the VBG 93 will be described in detail.
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  • 97
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    Lasers in medical science 4 (1989), S. 329-340 
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract In general “Dose” by itself as an amount of energy is not a relevant parameter for the description of penetration by non-ionizing (laser) radiation. But dosimetry should not only include the measurements of relevant parameters and its relatioships to tissue effects but also include all possibilities of measurements and relationships which help to control the biological result on tissue by irraditation with non-ionizing laser radiation.
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  • 98
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    Lasers in medical science 4 (1989), S. 361-365 
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract In this paper the Author presents the guidelines of laser therapy in the Lisbon Institute of Ophthalmology, with spetial referance to the laser parameters, the ocular pathology imposing laser therapy and the necessary conditions for safety of the patient and of the doctor, operating with laser.
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  • 99
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    Lasers in medical science 4 (1989), S. 41-61 
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract A review is presented of Raman spectroscopic applications in the study of laser-tissue interactions. The basic definitions of Raman spectroscopy are given with emphasis to those concepts and techniques which are relevant to biomedical material characterization. Representative problems from current literature will be discussed, like atherosclerosis of human vessels, eye lens aging, formation and diagnosis of cataracts.
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  • 100
    ISSN: 1435-604X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract Three different lasers were compared under the same conditions for their effectiveness at producing photodynamic damage to normal colon following sensitization with aluminium sulphonated phthalocyanine (AlSPc). One laser was an argon ion pumped continuous wave (CW) dye laser and the other two were pulsed at 10 kHz (copper vapour laser pumped dye laser, and 5 Hz (flashlamp pumped dye laser). The CW and 10 kHz laser were equally effective at producing damage. The 5 Hz laser failed to produce a photodynamic effect, although occasionally caused a photomechanical effect when the laser fibre was placed touching the colonic mucosa. Quantitative analysis suggests that the high energy pulses of the flashlamp pumped dye laser saturate AlSPc, so very little of the available energy can be used to produce a photodynamic effect, in contrast to the two other lasers which do not produce saturation conditions.
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