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  • Industrial Chemistry  (1,073)
  • Cell & Developmental Biology  (787)
  • 1995-1999  (878)
  • 1965-1969  (982)
  • 1997  (878)
  • 1968  (467)
  • 1967  (515)
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  • 1995-1999  (878)
  • 1965-1969  (982)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 121 (1967) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 2
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An electron microscope study of oocyte maturation in the mouse revealed that some mitochondria undergo gradual transformation in their ultrastructural appearance. In very young oocytes these mitochondria were already found to contain vacuoles, one in each such organelle. In somewhat older oocytes more mitochondria displayed vacuoles which generally appeared to be getting larger. These intramitochondrial vacuoles were found to be essentially optically empty structures surrounded by a single membrane. In favorable sections someof the developing vacuoles had a bottle-shaped appearance, the constricted end of which was attached to the inner limiting membrane of the mitochondrion. With further maturation of the oocytes vacuoles having a pear-shaped appearance became evident. An hypothesis was presented outlining the mode of formation of these vacuoles by expansion of the individual cristae. Intramitochondrial transformations occuring during both oogenesis and spermatogenesis in mammals were reviewed.
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  • 3
    Electronic Resource
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 121 (1967), S. 103-133 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The monotypic perciform suborder Luciocephaloidei possesses the following, previously unknown, salient morphological characters: a third joint, the nasopalatopterygoid, between neurocranium and suspensorium; a toothless and dorsally exposed prevomer; no pharyngeal processes on either parasphenoid or basioccipital; a tympanum-covered foramen exoccipitale in the saccular bulla as a hearing organ; a gular ossification; a craniovertebral joint with small exoccipital condyles widely separated from the basioccipital condyle; and a large physoclystic swimbladder with a notable caudal extension. The seemingly functionless gular-like mental ossification is considered a paleomorphic structure with a neogenetic development. Attenuation in longitudinal growth is evident in the derivatives and dermal additions of the mandibular arch and nasal capsule while other regions of the head have remained independent. The primary adaptive significance of the attenuation in the growth of the entire preorbital region is the accomodation of oral incubation. Secondarily the elongate jaws increase both the speed and grasping range of the bite in prey catching. The degree of jaw protrusion depends mainly on the length of the maxillary. The exaggerated length of the ascending processes of the premaxillaries may be the result of a positive differential growth rate within one growth field. The meaning of the preponderance of parallel-fibered cranial muscles is discussed in respect to holding functions, greatest possible excursion of the insertion with minimum loss of force, and muscle fiber length. Based on the overall morphology, the monotypic suborder Luciocephaloidei is retained.
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  • 4
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 121 (1967), S. 135-157 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Osteology, myology and motion analysis of the head of the anabantoid fish Helostoma temmincki, a specialized filter feeder, has revealed six functional units: neurocranium, suspensory apparatus, opercular apparatus, hyoid apparatus, branchial apparatus and pectoral girdle. Interactions between the functional units take place through four couplings involved in opening and protruding the jaws. The first coupling is activated in the beginning of the opening cycle by the levator operculi muscle through the opercular apparatus, interoperculomandibular ligament and mandible. The second is activated during feeding by contraction of the sternohyoideus through the hyoid apparatus, interopercular, interoperculomandibular ligament and mandible. The third coupling is active during feeding and “kissing” by contraction of epaxial muscles through mediation of the neurocranium to the jaw apparatus. The fourth coupling is the only one active during air intake and involves contraction of the levator arcus palatini which abducts and rotates the suspensory apparatus forwards, causing the mandible to drop. The retention of isolated ancestral characters during mosaic evolution are explained in terms of the maintenance of couplings which represent functional associations of seemingly remote structures. When natural selection acts on one component of a functional unit or coupling, it essentially acts on all associated elements simultaneously causing character complexes to evolve in common evolutionary trends. It is feasible that functional analysis can separate primary from secondary evolutionary trends.
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  • 5
    Electronic Resource
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 122 (1967), S. 89-114 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Large dogs are able to deliver a powerful bite that generates considerable stress in the anterior, prehensile part of the jaws.In the upper jaw most of the biting force is borne by the anterior teeth. The palatal mucosa provides little resistance to deformation. It is easily compressed and rather mobile.In the lower jaw, the mucosa covering the upper surface of the symphysis receives a sizeable portion of the biting force. It is firmly attached to the underlying bone and possesses special connective tissue arrangements that enable it to transduce locally applied pressure to tension distributed over a broad area.
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  • 6
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Goldfish testes were nutritionally regressed in about 115 days regardless of season and without controlled light or temperature. A gonosomatic index (testes weight ″ 100/body weight) of the regressed fish was about one tenth that of spawning fish. The regressed testes were primarily composed of spermatogonia, spermatocytes, and connective tissue. Fish testes were maintained in a regressed state for over 200 days with no change in gonosomatic index. Fish with regressed testes appeared to be in a state of “pseudohypophysectomy” with respect to gonadotropin. Pituitary replacement and a diet of 5% of the body weight per day initiated spermatogenesis and brought the regressed testes to functional maturity in one month. The results suggest that spermatogonial proliferation and the maturation of sperm have different regulatory requirements.
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  • 7
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 123 (1967), S. 1-16 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A theoretical analysis is made of the mechanical advantages of exoskeletons and endoskeletons. More complicated and realistic loading systems are considered than have been by previous authors. For all cases involving static loading, an exoskeleton would seem to be advantageous, but sometimes the advantage is quite small. If impact is considered, the advantage of exoskeletons becomes very much reduced, even on theoretical calculations; and it is likely that in life the advantage may be converted to a disadvantage, particularly in large active animals such as vertebrates.
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  • 8
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 123 (1967), S. 43-61 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Silver stained Cordylophora were examined by light and electron microscopy, which provided a general picture of nerve cell forms and distribution for comparison with electron micrographs of osmium-fixed tissues from the same hydroid. Muscle, nerve and neurosensory components were studied in the nectophore of Nanomia (O. Siphonophora) and in the hydromedusae Sarsia and Euphysa by means of vital staining and optical and electron microscopy of epon sections; particular attention was given to relationships and interconnections between the cellular elements of the two marginal nerve rings. Mitochondrial size, numbers and types of vesicles and the occurrence of neurotubules and of parts of sensory cilia may provide useful ultrastructural clues for recognizing nerve elements, but serial sections are often needed to make identification conclusive.In Cordylophora and Nanomia, some neurites contain massed A vesicles (membrane-bounded dense granules) suggestive of neurosecretion (cf. reports on Hydra). However, a small type of A vesicle also occurs at synapses in Sarsia, indicating a probable role here in junctional transmission. Vesicles occur on both sides of some synapses (as previously reported for Cyanea) but on one side only in others, these being the first examples of polarized junctional ultrastructure in coelenterates.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Morphology 123 (1967) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 10
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 123 (1967), S. 231-249 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A correlated light and electron microscope study was made of lymphocyte-epithelial relations in the appendix of normal rabbits, ranging in age from one week to ten months. The lymphocyte migration into the epithelium was very slight at one week. The lymphocytes were increased considerably in number from two weeks to three months, grouping into unique nests in the epithelium. The basement membrane began to be penetrated by migrating cells at one week and bacame discontinuous in older animals. At one and two weeks, the epithelial cells contained glycogen, which disappeared at three weeks. Degenerating cells as well as bacilli were found in the epithelial cells and in the macrophages of the nodules. The varied appearance of bacilli in the macrophages indicates that they were being digested. The lymphocytes in the epithelium were larger, having less crowded cytoplasm as compared with those in the lymph nodules. Many lymphocytes were in deep folds of epithelial cells. There was evidence favoring an intracellular position for some lymphocytes in the epithelium.
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  • 11
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: White Leghorn eggs were incubated to desired prevasular stages. Each embryo, upon its intact yolk and surrounded by albumen, was rolled from the shell into a sterile 50 ml beaker. In an uppermost position, the blastoderm was lightly stained with neutral red. Three types of localized cuts were made as indicated below, and the beaker-embryo unit placed in a sterile humidified chamber for further incubation. Results: (1) Unilateral cuts adjacent to the body and parallel with its axis blocked or suppressed formation of the vitelline artery on the cut side, even though healing occurred; (2) A specific site was found in the area pellucida opposite the sinus rhomboidalis which, when lightly cut perpendicular to the body axis, resulted in blockage or shifting of the final junction between aorta and vitelline artery as far cephalad as the thirteenth somite level. Formation of a dual final junction with the aorta also occurred. (3)Transverse cuts through the body axis and into the area pellucida bilaterally, frequently resulted in bilateral blockage or bilateral shifting. In still other cases, growth of a functional loop around the cut to connect the aortae in anterior and posterior segments with the vitelline artery were observed. Other unique circulatory patterns are described.Apparently, slight interference with the capillary plexus which precedes the vitelline artery causes anomalous development. Circulation is considered a major factor in arterial differentiation. Cutting probably alters the plexus and relation of its components to the onset of blood flow.
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  • 12
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Branches of the coronary arteries of normal human hearts, supplying both atria and ventricles, were found by fine dissection and have been named rami atrioventriculares. They comprise atrial branches from ventricular arteries and ventricular branches from atrial arteries. Their incidence was 74% in the 50 adult individuals studied. The subjects had committed suicide with a poison which did not damage the coronary arteries. The atrioventricular branches constitute communications across the coronary sulcus, thus establishing a continuity between the atrial and the ventricular arterial supply. Therefore, there is not invariably a sharp demarcation of blood supply between atria and ventricles, as has been commonly taught. Neither atrial nor ventricular branches consist exclusively of ascending and descending branches of the coronary arteries, as has been assumed. Atrioventricular branches can play a role in collateral circulation and may, in individuals who are born with them, provide an explanation for some of the variability in signs and symptoms incidental to heart attacks.
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  • 13
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The paper deals with the development of the salivary gland system in Melipona quadrifasciata anthidioides, which begins in the prepupal stage. The silk glands degenerate by autolysis at the end of the larval stage. Degeneration is characterized by cytoplasmic vacuolization and pycnosis of the nuclei of the secretory cells. The glandular secretory portion of degenerated silk glands separates from the excretory ducts. The salivary glands develop from the duct of the larval silk glands. The thoracic salivary glands develop from the ducts of the secretory tubules and the head salivary glands from the terminal excretory duct. The mandibular glands appear in the prepupa as invaginations of mandibular segments, and their differentiation to attain the adult configuration occurs during pupation. The hypopharyngeal glands have their origin from evaginations of the ventral anterior portion of the pharynx. A long tubule first appears with walls formed by more than one cellular layer. Then some cells separate from the lumen of the duct, staying attached to it by a cuticular channel in part intracellular. The initial duct constitutes the axial duct, in which the channel of the secretory cells opens. During the development of salivary and mandibular glands, they recapitulate primitive stages of the phylogeny of the bees. During the development of salivary glands system, mitosis accounts for only part of the growth. Most of the growth occurs by increase in size of cells rather than by cell division. In brown-eyed and pigmented pupae six days before emergence, the salivary gland system is completely developed, although not yet functioning.
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  • 14
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The caudal anatomy (caudal skeleton, musculature, vascularization, innervation, and urohypophysis) and swimming behavior of three clupeiform and three perciform fishes: Elops hawaiensis (Cupeiformes: Elopidae), Oncorhynchus nerka (Cupeiformes: Salmonidae), Chanos chanos (Clupeiformes: Chanidae), Kuhlia sandvicensis (Perciformes: Kuhlidae), Apogon menesemus (Perciformes: Apogonidae), and Gnathanodon speciousus (Perciformes: Carangidae), were studdied. The taxonomic significance of caudal structures was determined and evaluated by detailed examination of differences in caudal anatomy. An interpretation of functional significance of these differences was attempted by relating them to observed differences in swimming behavior. The swimming behavior was studied by the observation of swimming activities of fish while resting or cruising and while feeding in the aquarium, and by an analysis of each frame of an 8 mm movie film of swimming activities.There are certain consistent and basic differences between all three species of the order Clupeiformes and all three species of the order Perciformes in respect to caudal structures. Although certain caudal structures show overlapping in number and/or complexity of arrangement, they seem to indicate more complex structural organization in Clupeiformes than Perciformes. The differences confirm the conclusion of others that the order Clupeiformes is more “primitive” than the order Perciformes.With respect to caudal structures of the three clupeiform species studied, E. hawaiensis is the most “primitive” and, of the three perciform species studied, K. sandvicensis is the most “primitive.”Caudal structural variations from one species to another are related to the mode of adaptation to swimming as well as to the evolutionary status of the species.
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  • 15
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 124 (1968), S. 37-77 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cells surrounding a wound in the integument of Rhodnius adults show an increase in RNA content, cytochrome oxidase and esterase activity. An excision in the integument is filled by blood which coagulates and is tanned into an insoluble membrane. The basement membrane of the adjoining epidermis acts as a self-sealing membrane and contracts to cover the excision. The epidermis is attached to the cuticle by the subcuticular layer which it resorbs and by pore canal filaments which are left behind as it migrates. The epidermis migrates as a sheet in contact with the cuticle then with the coagulated blood and basement membrane which cover the excision. Blood cells migrate individually into an excision and do not adhere to a surface in the process. Microtubules cannot be identified with movement. Both epidermal and blood cells remove the cells killed by wounding as evidenced by the appearance of coated vesicles and phagocytic bodies in both cell types. The reconstituted integument consists of a surface membrane in which the layers of the epicuticle are not distinguishable, a nonlamellate cuticle secreted by an epidermis which also appears to secrete the new basement membrane.
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  • 16
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 124 (1968), S. 295-311 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The development of intraneural vessels was studied in response to an induced hypermorphosis of neural tissue inthe midbrains of 38 chick embryos ranging in age from three days through 14 days of incubation. The pattern of vascularization was compared with that of normal chick embryos at comparable stages of development. In the experimental embryos, the increase in mitotic figures along the ventricular borders of the mesencephalon is accompanied by the establishment of an endoneural plexus approximately one day earlier than is the case during normal vascularization of the midbrain. This plexus also penetrates more deeply and extensively into the ependymal layer. Surface vessels and intraneural vascular elements are dilated, and the cerebrospinal fluid contains varying amounts of blood released from large intraneural vessels which protrude into the ventricle. The most prominent cerebrovascular effects seem to occur between the fourth and eighth days of incubation. Thereafter, the cerebrovascular pattern becomes more normal except for relatively few isolated hemorrhagic areas.
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  • 17
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 124 (1968), S. 345-351 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The eye of Haideotriton wallacei is more reduced histologically than those of othe troglobitic salamanders. The tiny eye is imbedded in a mass of adipose tissue. No extrinsic eye muscles are present. A rudimentary lens is present in about half of the eyes examined. In two instances the lens is surrounded by a small chamber; most eyes lack a chamber. The retina and iris are relatively undifferentiated. The relatively massive retina lacks rods and cones, an outer plexiform layer and subdivided nuclear layers. A tiny optic nerve runs to the brain.
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  • 18
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The aesthetascs, short thin-walled pegs on the antennule flagella of Coenobita clypeatus, a terrestrial hermit crab, are similar to those of other decapod crustacea in containing the dendrites of many bipolar neurons whose cell bodies are grouped in spindle-shaped masses beneath the bases of each hair. The dendrites contain rootlets, basal bodies, and cilia, which divide dichotomously before entering the aesthetasc, so that within the hair, each cilium becomes represented by a group of slender branches.The aesthetascs themselves are short, blunt, and partially recumbent so that each has an exposed and an unexposed side. The cuticle on the exposed side is thinner and more tenuous than that on the protected side, and the dendrite branches are concentrated just underneath. The protected side, on the other hand, is lined with nondendritic supporting cells, and the cuticle is thicker, more lamellar, and probably less permeable.All dendritic elements proximal to the dendrite branches are enclosed within the main body of the antennular flagellum, and the initial segments of the cilia lie within a vacuole. In these respects, the aesthetascs of Coenobita resemble the thin-walled pegs on insect antennae more than they do those of the marine decapods thus far examined. This convergence in the terrestrial forms may be in response to the need to conserve water.
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  • 19
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The Brittle Star digestive system is composed of buccal, pharyngeal, esophageal and stomach cavities. The buccal and pharyngeal cavities are lined by columnar cells covered by a cuticle, and are apparently concerned with mucous production. Coelomocytes and tall columnar cells are described in the esophagus and stomach epithelia. The columnar cells are adapted for nutrient absorption, enzyme synthesis, and lipid storage. Nerves are found beneath the epithelia within a connective tissue layer. Smooth muscle and coelomic layers lie external to the connective tissue layer. The coelomic layer lines a perivisceral space and has diverse modifications of its perivisceral surface; a pedicle-cuticle modification perhaps having general significance in echinoderms.
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  • 20
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 125 (1968), S. 61-70 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Two complete composite photographs of the optic nerve of Limulus, made by electron microscopy, reveal the presence of neurosecretory granules in the large axons of the rudimentary eye neurons. The number of intermediate sized, (3-7 μ), of eccentric cells corresponds with the number of ommatidia as expected, but only their sheath of Schwann cells show an intimate interfolding. Based on the number of fine axons within the nerve each ommatidium has an average of 12-13 retinular cells. The diameter of their fibers is between 0.2 and 3 μ although the majority are between 1 and 1.5 μ. They are aggregated into bundles of six to seven fibers by the sheath cells although some bundles contain only two, others as many as 181 fibers. There is no indication in these studies that retinular cell axons within a bundle are associated with the same, adjacent, or other pattern of ommatidia. The photographs suggest that physiological activity in retinular cell axons might be detected most easily in the smallest bundles because they contain the fewest, but the larger retinular cell axons.
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  • 21
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The lateral and ventral external surfaces of the third and fourth abdominal segments were described and muscle attachments were correlated with surface indentations of the larva. The proleg of this species has a symmetrical planta with a complete circle of crochets. Furthermore, it differs externally from the grasping type of proleg in having a largely membranous coxal region confluent with the body wall, and a relatively large subcoxal lobe.The body wall musculature and innervation of the third and fourth abdominal segments are similar in many respects to those described for other lepidopteran larvae to which they are here compared, but differ from most because of the simpler structure of the prolegs which lack highly developed adductor muscles. Like most muscles innervated by the ventral nerve, the principal plantar retractors of these two segments cease to function in the first day of the pupal stage and have completely degenerated by the forty-fifth hour of pupal life. The ventral nerve retains its four primary branches in the adult, in which many smaller rami can be traced to the cuticle and to the neoblastic body wall muscles.
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  • 22
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The gross anatomy of muscles, the topography of nerve tissues, and the histology of the pregenital abdominal glands of Nomia melanderi Ckll. are reported in detail. The movable and fixed points of muscle attachment were utilized in establishing a system of nomenclature for a typical abdominal segment. Names of nervules correspond to those of the tissues they innervate. The points of attachment of muscles of the fifth abdominal segment are essentially the same in both Nomia and Apis, except for the second tergo-sternal muscle which, in Nomia, has shifted its point of movable attachment to the membranous integument in front of the intersegmental membrane gland where it helps in relasing glandular secretion. The general plan of the nerves in the fifth abdominal segment in Nomia is more diffuse than in Apis, but there is no difficulty in establishing homology between the nervules of the two species. A pair of intersegmental stretch organs was found in abdominal segments 3-6. Glands of the sixth intersegmental membrane possess a reservoir with peripheral pouches both of which are absent in those of the fifth. Both types of glands have neither closing nor opening mechanisms, and neither is innervated. Release of glandular secretion is accomplished by the action of the tergo-sternal muscles.
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  • 23
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    Journal of Morphology 125 (1968), S. 253-257 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This report describes the capacity of larvae of the Leopard frog, Rana pipiens, to regenerate pineal tissue after embryonic pinealectomy, and presents experimental evidence for frontal organ dependence on the epiphysis. In addition, it was found that the “brow spot,” a pigment-free region located in the epidermis betweeen the eyes, is dependent on the frontal organ for its formation. This report also substantiates that the absence of the pineal organ in larval animals does not affect growth or development through metamorphosis.
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  • 24
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    Journal of Morphology 125 (1968), S. 315-328 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Interest in the structure of the antennae of the Florida Queen butterfly arises from the finding that a pheromone is active in their courtship. Light and electron microscopic techniques were used to study the sensilla on the antennae and three types of sensilla with perforated walls were identified. The most common of these are short, thin-walled pegs which are distributed over most of the antennal surface. Long, curved, thin-walled pegs occur in patches on the inner medial antennal surface. Multiple coeloconic sensilla are present having up to 50 pegs in one sensillum. On the outer 28 flagellar subsegments there are two such sensilla per subsegment. In addition there are on the antennae long, thick-walled hairs which are mechanoreceptors and probably also contact chemoreceptors. Sunken pegs, the function of which is not known, occur on the antennae. Grooved sensilla were found with the electron microscope but could not be identified with the light microscope. There was no indication of sexual dimorphism in sensilla types or numbers on the antennae.
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  • 25
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    Journal of Morphology 125 (1968), S. 403-417 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Succinic dehydrogenase, NAD and NADP diaphorases, choline oxidase, d-amino acid oxidase, α-glycerophosphate dehydrogenase, glutamic dehydrogenase, lactic dehydrogenase and aldolase were identified histochemically in the esophagus and proventriculus of the developing chick embryo. In general, the deep glands of the proventriculus reacted more strongly than the epithelium and mucous glands of the esophagus to the tests. The intensity of the enzymatic activity seems correlated with the number of mitochondria and metabolic activity of the cells.
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  • 26
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    Journal of Morphology 126 (1968) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 27
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    Notes: Two kinds of tactile hairs, plain and wavy, thick-walled chemoreceptors and two types of thin-walled chemoreceptors are present on the antennal flagellum of Gromphadorhina portentosa males. These are described and their location on the antenna noted. Females of this species have plain tactile hairs and the same types of chemoreceptors as do the males but wavy tactile hairs are absent. The antennal sense organs of a few specimens of five other species of cockroaches  -  Periplaneta americana, Blatta orientalis, Supella longipalpa, Pycnoscelus surinamensis and Diploptera punctata  -  were also examined. All lacked tactile hairs but were provided with thick-walled chemoreceptors and with two types of thin-walled chemoreceptors similar to those described for Gromphadorhina portentosa.
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  • 28
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    Notes: Pulse labeling with tritiated thymidine has permitted the identification of a progenitor cell compartment at the base of the hypostomal glandular ridges of the marine hydroid Podocoryne carnea. This progenitor compartment produces cells which move out on the ridges differentiate as digestive gland cells, and are ejected into the gastro-vascular cavity. Spherous gland cells of the body appear not to be part of this circumscribed replacement system. There appears to be no proximal movement of gastrodermal cells in the body of this marine hydroid.
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  • 29
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    Notes: Among eight species of mammals in this study (cattle, sheep, pig-tail and rhesus monkeys, rabbit, pig, rat, and dog) four basic patterns of anatomical structure at the uterotubal junction are described. The classification of types is based upon the presence or absence of an intramural portion of the oviduct and of isthmal folds or plicae projecting into the lumen of the uterine cornu.Histological variations are reported for three tissues: epithelial and connective of the mucosa and smooth muscle of the tunica muscularis. In the epithelium during the estrous cycle the differences recorded include: (a) absence of ciliated cells in the distal end of the oviduct in rat and dog; (b) variations in ciliated and nonciliated cells in (1) cell height, (2) location, shape and stainability of the nucleus, and (3) in amount and stainability of apical cytoplasm; (c) presence of lymphoblast-like cells which appear to migrate through the epithelium from the lamina propria. The connective tissue of the mucosa, as a circular layer and as cores for the mucosal folds, shows variations in thickness and in relative density of cells and fibers of the matrix. Emphasis is given to the presence of an inner longitudinal layer of smooth muscle in the tunica muscularis of the distal oviduct in six of the eight species.
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  • 30
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    Journal of Morphology 125 (1968), S. 259-279 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The structure of cells in the colleterial glands of the Cecropia silkmoth was examined. Morphologically and functionally the gland is divided into two regions, a tubular one in which columnar protein-synthesizing cells are located, and an expanded region in which flattened cells with very different structure are most prominent. The fine structure of the latter cells which are presumed to secrete a phenolic glucoside, closely resembles that of cells described in the colleterial glands of orthopterans. The protein-secreting cells have many features normally associated with pancreatic acinar, and other cells of similar function. Among these are extensive rough endoplasmic reticulum, an elaborate Golgi complex, and a modest number of mitochondria. Other features which are less usual in cells of this type are an elaborate secretory apparatus consisting of a cuticular tubule inserted into a microvilli-lined cavity at the apical end of the cell, and large numbers of cytolysomes, myelin figures, and lipid droplets. A chitogenous cell with a very distinct and specific type of ultrastructure is found associated with the secretory cell. This cell type is attached to the cuticular elements of the gland, and the main features of its cytoplasm are extensive bundles of microtubules which presumably serve as supportive elements for the secretory cells.
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  • 31
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    Journal of Morphology 125 (1968), S. 329-365 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Embryonic chick myocardium (stages 8+ to 12-) was studied by light and electron microscopy. The myocardium, which is initially comprised of radially oriented cells with large intercellular spaces gradually becomes more tightly packed. Intercellular spaces decrease and the cells assume a circumferential orientation. Myocardial cells remain epithelial throughout formation of the functional tubular heart and specialized epithelial junctions (apical junctional complex or terminal bars) undergo modification to form intercalated discs. Embryonic myocardial cells contain large amounts of free ribosomes and particulate glycogen, the latter often associated with portions of granular reticulum. Unlike developing skeletal muscle. The amount of granular reticulum contained in the myocardial cell cytoplasm is large and, along with a hypertrophied Golgi apparatus, suggests that these cells may have a secretory function. These organelles persist during the initial period of fibril formation. Myofibrils apparently form from non filamentous precursor material and not by alignment of sequentially synthesized components.
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  • 32
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    Journal of Morphology 125 (1968), S. 379-401 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The characteristic anoline climbing organ consists of a number of lamellar scales, on whose outer scale surface are numerous keratinized setae which contact the substrate. These setae are derived from the Oberhautchen of the epidermal generation, and as such are renewed and shed periodically along with the rest of the epidermal material. The histological development of the setae is described, and modifications of the surrounding elements are noted. The relative lengths of the setae and their congregation to form a pad unit poses certain mechanical problems during morphogenesis, simply in terms of accommodation between the functional outer epidermal generation and dermal core of each lamella. Regression of the dermal core and a distal migration of some cells permits accommodation within the lamella for the distal aspect of the Oberhautchen layer, or free margin. Additionally, changes in the gross shape of the lamella occur throughout the sloughing cycle, and a swelling of the cells of the lacunar tissue results in a gap between the stratum corneum of inner and outer epidermal generations. There is a considerable amount of variation in mitotic activity between the germinal layers of opposite sides of the lamella.
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  • 33
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    Notes: Function and ultrastructure of the excretory organs (antennal glands) of the shore crab Uca mordax were investigated. The crabs were maintained at three different salinities: 50%, 100% and 200% seawater. In spite of previous reports to the contrary, the investigation showed that the powerful osmoregulatory ability found in Uca mordax is not due to participation of the antennal glands. Freezing point depression of urine under all conditions was found to be slightly less than that of the hemolymph, indicating a slightly hypoosmotic urine. It was further found that the antennal gland is extremely effective in resorbing sodium from the filtrate. The higher the salinity to which the crabs were acclimated the lower the sodium concentration in the urine. No water was resorbed from the filtrate as shown by the fact that the inulin U/P ratio remained unity regardless of the salinity to which the crabs were adapted. Electronmicroscopy of the antennal glands revealed that the coelomosac cells are similar to the podocytes described in the crayfish by Kümmel ('64), and the coelomosac appears to be a typical filtration organ. The cells of the labyrinth showed brush border and very elaborate basal infoldings with numerous mitochondria. The deep cytoplasmic infoldings which represent interdigitations with neighboring cells may be correlated with the effective sodium reabsorption in the labyrinth, but apparently not with water movement.
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  • 34
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    Journal of Morphology 126 (1968), S. 31-65 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Mitochondria in early spermatids of many insects aggregate and form a round body, the nebenkern. The nebenkern undergoes a structural differentiation and then divides into two separate equal-sized bodies. In the present study, nebenkerns of Murgantia histrionica, a Hemipteran insect, were reconstructed using electron micrographs of serial sections to determine how the mitochondria transform into the two separate bodies.Newly formed nebenkerns are made of one piece, an anastomosis of rod-like segments. Some segments interconnect to join networks of rings. Each network interlocks with another similar network, but networks which interlock are connected with each other by other segments of the nebenkern. Later, the entire nebenkern is made of two unconnected and interlocked networks of rings. The nebenkern appears to remain bipartite during subsequent differentiation. Since the two pieces are interlocked, breaks must occur before the pieces can separate. As breaks occur, each network transforms into a set of curved sheets, producing a nebenkern made of four concentric layers. The three outer layers are each made of two curved sheets which surround a bipartite central core. The surface sheets meet at a furrow in the surface of the nebenkern; segments in each layer are roughly symmetrical with each other about the plane in which the furrow lies. Rod-like segments join alternate segments. The number of layers then decreases to three, and later, to two. These nebenkerns resemble four-layered nebenkerns, but fewer connections between alternate segments are present. The two pieces constituting the nebenkern probably separate after most of the latter connections disappear. Hypotheses to account for the observed changes in nebenkern structure are presented.
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  • 36
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    Journal of Morphology 126 (1968) 
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  • 37
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    Notes: The forms of the tectorial membrane and its connections to the ciliary tufts of the hair cells have been studied in detail in 18 species of lizards and, less thoroughly, in three others. This group, which represents eight lizard families, exhibits three forms of tectorial membrane: a complete form that connects to all parts of the auditory papilla, an abbreviated form that makes this connection only in one limited region of the papilla, and a dendritic form in which the distal portion of the membrane subdivides into strands reaching all the hair cells.Sometimes the tectorial membrane connects directly with the tufts of the hair cells, but more often it makes this connection through intermediary structures. Seven types of such intermediary structures have been identified (if we include the sallet, which is not “intermediate” in a strict sense).Detailed descriptions are given of the various forms of tectorial membrane structures and their variations along the auditory papilla in 12 lizard species. The description for Iguana iguana is offered as representative of the iguanid pattern found in ten members of this family.Consideration is given to the functioning of the tectorial membrane, and also of the sallet, in the process of hair-cell stimulation.
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  • 38
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    Notes: There were no lymphatic vessels and lymph nodes demonstrable in adult and larval Rana catesbeiana by a method that adequately demonstrated the same in mammals. Although the parenchymal arrangement in the lymphomyeloid organs is not exactly the same as in mammalian hemal nodes, nonetheless the vascular patterns of the lymph glands and jugular bodies are prima facie evidence that they function as blood-filtering organs among other probable functions. The vascular pattern of the lymph gland is that of a rete mirabile, particularly a venous portal system, inasmuch as the afferent and efferent vessels are venous in character and interposed between them is a labyrinth of sinusoids. This is not the case, though, in the adult organs. The vascular pattern of the jugular bodies is very much like the spleen, viz., artery-capillary-sinusoid-vein sequence. It is doubtful, however, if the propericardial and procoracoid bodies ever filter blood, because the smallest blood vessels in them are capillary in type Because of the absence of a well-defined capsule in some parts of the propericardial body, similarly to lymphoid follicles, especially in the mammalian gastrointestinal tract, it is probable that it filters tissue fluid. The last two organs are apparently mainly blood cell-forming organs. It is inferred from the vascular connections of the larval and the adult lymphomyeloid organs that they are not genetically related. This aspect was analyzed from earlier developmental data, but actual follow-up of the larval organs to the adult stage is still in progress.
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  • 39
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  • 40
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    Notes: Histological serial sections of spleens from the adult raccoon (Procyon lotor) and woodchuck (Marmota monax) were processed for microscopic examination. Observations related to various aspects of the internal vascular pattern in the spleen of the raccoon and woodchuck demonstrated features that were characteristic of the respective animal. The spleen of the raccoon possess well-developed ellipsoid sheaths, whereas these same structures were not as prominent in the spleen of the woodchuck. The spleen of both mammals examined demonstrated the presence of an anastomosing series of venous sinuses within the red pulp tissue and may be classified as sinusal in nature.
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  • 41
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    Journal of Morphology 121 (1967) 
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  • 42
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    Journal of Morphology 121 (1967), S. 55-70 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Light and electron microscopical studies allow a descriptive account to be given of he morphogenesis of the egg chamber of Drosophila melanogaster. The study demonstrates that the mitotic products of a single cystoblast generate a branching chain of 16 inter-connected cystocytes. Two specific cystocytes enter meiotic prophase, while the rest become nurse cells. The two pro-oocytes form synaptinemal complexes in their nuclei. However, one of the two cells later switches back into the nurse cell developmental pathway. The elongation of the synaptinemal complexes is described, and estimates are made of the time involved in their formation. These complexes continue to be synthesized long after the DNA replication which gives the oocyte its 4C DNA content. This finding implies that at least some genetic crossing over follows DNA replication. Evidence is presented that cells undergoing crossing over are most efficient in repairing radiation-induced chromosomal breaks. Suggestions are given as to the mechanisms by which (1) cell division is inhibited once 16 cystocytes are formed, (2) the future cleavage planes of cystocytes are programmed, (3) the pro-oocytes are differentiated from nurse cells, and (4) the oocyte is chosen from the twin pro-oocytes. The contrasting behaviors of the oocyte and nurse cell nucleoli are described. During oogenesis nucleolar synthesis of ribosomal RNAs is suppressed in the oocyte and concurrently stimulated in the nurse cells. It follows that the nurse nuclei are the major sources of the prodigious quantities of ribosomes found in the ooplasm of the mature oocyte.
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  • 43
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    Notes: The enigmatic lophophoral organs of phoronids are demonstrated to by accessory sex glands. In the hermaphroditic Phoronis vancouverensis, each mature adult has two pairs of such glands: female nidamental glands that facilitate the temporary brooding of the embryos and male accessory spermatophoral organs that function in spermatophore elaboration. Functional males of Phoronopsis harmeri have complex accessory spermatophoral organs while females of this non-brooding dioecious species lack accessory sex glands.In both species, coelomic spermatozoa are accumulated within the nephridia and passed en masse into the partially enclosed spermatophoral organs. There each sperm mass is molded into a characteristic shape and encased in one or more membranes before release as a spermatophore into the ambient water. The structure of the spermatophore is characteristic for each species and is correlated with the functional morphology of the distinctive spermatophoral organs.The existence of spermatophores, the non-primitive nature of the spermatozoa, and additional features of reproductive biology dictate that fertilization is internal in these species, but attempts to determine the means of sperm transfer proved futile. Reproductive potential and behaviour of male Phoronopsis, means of gamete segregation in Phoronis, and general pattern of reproductive biology for both phoronids were also studied.
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  • 44
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    Journal of Morphology 121 (1967), S. 19-28 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In the column of hydra, tissues continually move away from a region located just underthe whorl of tentacles. Above this subtentacular region, tissues proceed into the hypostome and tentacles; below it tissues pass into the buds or continue down the stalk. These movements represent a steady state pattern of tissue renewal in which column growth is balanced by tissue loss at the body extremities. But the existence of a subtentacular zone in which tissue appears stationary does not necessarily indicate that growth is restrictedto this region, as is commonly stated.The body column of hydra can be viewed as an expanding cylinder whose elongation is balanced by tissue loss at the two ends. In such a body there must be one region from whichtissue appears to emanate, regardless of how growth is distributed along the cylinder. Only the rates at which tissues move will be characteristic of the underlying growthpattern. In Hydra littoralis, the measured rates of tissue movement down the gastric column are consistent with the distributions of mitotic figures, which indicate that growth is spread out along the column.
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  • 45
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    Journal of Morphology 121 (1967), S. 71-80 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Profiles of fresh chick lenses of 5, 8, 11, 14, 17 and 20 days embryological age were photographed and from tracings of these profiles, volumes were calculated using a formula for volume of a solid of revolution. Accurate volumes were also obtained by assuming the lens to be two half oblate spheroids of different minor axis, or, better, two half-solids of profile (x/a)k + (y/b)k = 1. In addition, the k in this equation described well the curvature of the lens profiles and thus provided a quantitative measure of developing lens shape. Lens diameter, depth and shift of equatorial diameter upon lens axis as a function of age were also studied.
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  • 46
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    Notes: Ductuli efferentes in four examined genera of family Didelphidae are organized into highly convoluted tubules, located within a conical body adjacent to the vascular plexus supplying the testis. They have yellowish-green color in the adult Didelphis virginiana and grey pink color in other genera. The ductuli efferentes in all examined animals are lined by very low columnar cells, covered by microvilli or booth cilia and microvilli.Histochemical analysis reveals several types of cytoplasmic granules in the genera examined. A single sperical body about 1.5 μ in diameter is found in epithelial cells of the four eyed opossum (genus Philander); this body stains positively for RNA and non-histone protein, and appears granular under the electron microscope, without a limiting membrane. Abundant cytoplasmic bodies in the Virginia opossum show strong reactions for SH groups; these granules may be responsible for the green color of the ductuli in this species. Ultrastructural studies show that the morphology of membrane limited granules in the ductuli of the examined genera is characteristic for each genus. Abundant pinocytotic vacuoles in the apices of these cells and a strongly positive alkaline phosphatase reaction suggest a marked absorptive activity of epithelial cells.
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  • 47
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    Journal of Morphology 121 (1967), S. 179-207 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Detailed descriptions are presented of morphogenetic and histogenetic events occurring in the membranous and bony labyrinths, sensory areas, acoustic ganglia, and acoustic centers of the hindbrain of the chick embryo, stages 11 to 45. Major morphogenetic changes occur between stage 11 and stage 30. During this period primordial ganglionic neuroblasts are segregated from the otic epithelium and differentiate into the bipolar neuroblasts of the vestibular and cochlear ganglia, neuroblasts of the alar plate in the acoustic region of the rhombencephalon differentiate, migrate, and are organized into acoustic nuclei, and the otic cup closes to form a vesicle which undergoes complicated structural changes to become a labyrinth. Morphological events in neural structures are closely followed by structural changes in the labyrinth. Minor morphological changes continue to occur through stage 44. The histogenetic period, stage 26 to 38, is the same for all parts of the acoustic apparatus. Minor histogenetic events occur up to hatching. In this period, differentiation of ganglionic and central neurones precedes differentiation of sensory cells.
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  • 48
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  • 49
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    Journal of Morphology 121 (1967), S. 223-239 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: With the help of PF and PAVB bulk-stained preparations and sections the neurosecretory system of Ranatra elongata has been described. Two medial, each of 9-10 cells, and two lateral, each of 3-4 cells, groups of neurosecretory cells have been observed in the protocerebrum. Only the A-cells have been found to be positive to PAVB histo- and cyto-chemical technique. Axons of the A-cells after traversing the proto- and deuto-cerebrum emerge from the tritocerebrum as the NCC I. The NCC I after bypassing the corpora cardiaca penetrate the aorta wall. There is no physical continuity between the corpora cardiaca and the NCC I and the two are separable. The NSM from the A-cells, transported by their nerve fibers, has been observed in the aorta wall. On the basis of large accumulation on NSM in the aorta wall the latter has been considered as the storage-and-release organ for the A-material. Corpora cardiaca are found to be devoid of A-material. Axons from the B-cells, after emergence from the tritocerebrum as the NCC II, have been observed to penetrate the corpora cardiaca. On the basis of ample amounts of B-material the glands have been considered as the storage-and-release organ for the B-material only.Observations are compared with results on related species and it is concluded that two independent organ complexes constitute the neurosecretory systemt of R. elongata. The A-cells, their pathways, the NCC I, and the aorta wall comprise the first; and the B-cells, their pathways, the NCC II, and the corpora cardiaca the second. The former is concerned with the elaboration, transportion, storage-and-release of the A-material and the latter with the B-material. Finally arguments are presented to include the aorta wall in the neurosecretory system.
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  • 50
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    Journal of Morphology 121 (1967), S. 311-321 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The anatomy of the ventral neck region of the scincid lizards Chalcides ocellatus and Scincus scincus is presented and is found to be similar to that of other lizards as described in the literature. The internal carotid artery arises by 3-5 roots from the dorsal side of the ascending limb of the carotid arch. During its first part, the internal carotid artery is completely divided into two nearly equal channels. The carotid sinus is more complicated in Chalcides than in Scincus. In lizards, it may be homologous to the carotid labyrinth of fishes and amphibians. Around the origin of the internal carotid artery are two kinds of epithelioid cells scattered in the adventitial connective tissue: a- large cells with rounded, faintly stained nuclei, and little, clear cytoplasm; b- cells with small darkly stained nuclei. Both kinds of cells appear to represent different levels of secretory activity. The number of the large cells increases with greater complexity of the carotid sinus. The cells also increase in size and number during summer (sexual period); this is especially true in younger animals. The epithelioid cells are considered to be homologous to the carotid body of higher vertebrates. The carotid sinus and epithelioid cells together form a closely interrelated system which may be intermediate between the carotid labyrinth of fishes and amphibians, and the carotid body of birds and mammals.
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    Notes: The fine structure of the nerve cell types in the white planarian Procotyla fluviatilis were described. Ganglion cells comprise the major portion of the brain. These cells are irregular in shape with several cytoplasmic processes and contain ribosomes, a sparse endoplasmic reticulum, microtubules, lysosomes, and a Golgi apparatus with numerous small vesicles. Granule-containing cells are situated in the peripheral regions of the brain and along the nerve cords. These cells contain ribosomes, rough-surfaced endoplasmic reticulum and a Golgi apparatus with associated dense granules. The granules occupy most of the cytoplasm and are ∼ 750A in diameter with moderately dense contents, ∼ 750A with opaque contents, and ∼ 1000A with contents of medium density. These granules are similar to those in the nervous systems of higher animals that contain epinephrine, norepinephrine, and neurosecretory substance, respectively. Each cell contains predominantly one type of granule although there is some intermixing of granules and intermediate types between the three most abundant granules. Small clear vesicles, resembling cholinergic synaptic vesicles, and all types of dense granules occur in the neuropil and within nerve endings.
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  • 52
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: A study of the ingestion of particulate matter from the pallial space located between the shell and the outer surface of the mantle of Isognomon alatus and Pinctada radiata was undertaken with the aid of the electron microscope. For this purpose colloidal thorium dioxide (thorotrast) was introduced into the pallial space for periods of 1-5 days after which time the mantle was excised and prepared for examination with the electron microscope. After 24 hours thorotrast micelles were observed in the pallial space, on the surface of the microvilli, in small pinocytotic vesicles between the bases of the microvilli, in vacuoles undergoing coalescence and finally in large dense bodies (lysosomes). Amoebocytes in the pallial space also participate in the removal of particulate matter in a manner similar to that described for the surface epithelium. During active ingestion the Golgi apparatus changes from a vesicular to a lamellar form. The method of ingestion observed in the surface epithelia and the amoebocytes is similar to the ingestion of protein and other particulate material reported for a variety of vertebrate tissues.
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  • 53
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    Journal of Morphology 122 (1967), S. 231-247 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The proventriculus of White Leghorn chick embryos (stages 29-45) newly-hatched chicks, and adult chickens were frozen, sectioned in a cryostat and treated histochemically to identify localizations of alkaline and acid phosphatase, adenosine triphosphatase, 5-nucleotidase, nucleotide-diphosphatase, non-specific glycerophosphatase, glucose-6-phosphatase, non-specific esterase and succinic dehydrogenase. Ribonucleic acid, proteins and acid mucopolysaccharides were identified in tissues fixed in FAA.Acid phosphatase, nucleotide-diphosphatase, adenosine triphosphatase, succinic dehydrogenase, ribonucleic acid and proteins were present in the cells of the deep glands at all stages of development. Alkaline phosphatase and 5-nucleotidase were found only in mesenchymal derivatives of the proventriculus. After the chick begins swallowing and digesting albumen, enzymatic activity increased and non-specific esterase became very reactive.The surface epithelium is covered with a mucous coat. Ribonucleic acid, non-specific esterase, acid phosphatase and nucleotide-diphosphatase were localized in the basal portions of the epithelial cells. The functional significance of these different patterns is discussed.
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  • 54
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    Journal of Morphology 122 (1967), S. 265-305 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Basal tail constriction occurs in about two-thirds of the species of plethodontid salamanders. The constriction, which marks the site of tail autotomy, is a result of a reduction in length and diameter of the first caudal segment. Gross and microscopic anatomical studies reveal that many structural specializations are associated with basal constriction, and these are considered in detail. Areas of weakness in the skin at the posterior end of the first caudal segment, at the attachment of the musculature to the intermyotomal septum at the anterior end of the same segment, and between the last caudosacral and first caudal vertebrae precisely define the route of tail breakage. During autotomy the entire tail is shed, and a cylinder of skin one segment long closes over the wound at the end of the body.It is suggested that specializations described in this paper have evolved independently in three different groups of salamanders.Experiments and field observations reveal that, contrary to expectations, frequency of tail breakage is less in species with apparent provisions for tail autotomy than in less specialized species. The tail is a very important, highly functional organ in salamanders and it is suggested that selection has been for behavioral and structural adaptations for control of tail loss, rather than for tail loss per se.
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  • 55
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    Journal of Morphology 123 (1967) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 56
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    Notes: Esophagi of White Leghron chick embryos, six days to hatching, were fixed in 5% glutaraldehyde in a cacodylate-sucrose buffer, postfixed in 1% OsO4, dehydrated and embedded in Araldite. Tissues were sectioned with glass knives and viewed with a Philips EM 100C. The epithelium in early stages of development is characterized by small intercellular spaces, few cell processes and few desmosomes. In contrast the differentiated epithelium contains numerous cell processes, large intercellular spaces and numerous desmosomes. Mucin appeared in the mucous glands at 17 days.
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  • 57
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    Notes: Adult sloths (Bradypus tridactylus) were studied by electrocardiography and by light and electron microscopy under normal conditions and under experimental conditions as provided for by injection of 2,4-dinitrophenol (DNP) and ether anesthesia. ECG's of the animals indicated heart rates of 45-71/min, which can be considered as the normal heart rate of the sloth under laboratory conditions. Under normal conditions, the contracted ventricular myocardium of the sloth exhibited (a) a wrinkled sarcolemma, (b) the usual pattern of myofibrils and of sarcoplasmic reticulum, (c) small mitochondria with spiked and branched, often anastomosed cristae, including a few small intramitochondrial dark bodies, (d) an amount of sacrcosomes smaller than the amount of myofibrils, (e) many glycogen granules, isolated, in the form of a chain, or as clusters, in subsarcolemmal, intermyofilamentous and perimitochondrial positions, (f) few multivesicular bodies and (g) large flat sections of the transverse tubular system.Injection of DNP (1 mg/kg) caused tachycardia. With ether anesthesia, the ECG showed monophasic action potential of myocardial injury and prolongation of inter or intraventricular condition. Electrically, the sloth's heart responded to hypoxia as do other mammalian hearts.The administration of DNP produced (a) derangement and reduction in number and length of the mitochondrial cristae, (b) disappearance of spikes, connections between the cristae and, consequently, the honeycombed arrangement, (c) increased matricial space in the center of mitochondria which was often filled with a grayish substance, (d) disappearance of small dark intramitochondrial granules, (e) depletion of glycogen particles and (f) few dilations in the sarcoplasmic reticulum.
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  • 58
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    Journal of Morphology 124 (1968), S. 167-179 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Latex endocranial casts, which may be made without damaging the skull, reveal much of the information provided by a hemisected skull. Examination of drawings of endocasts superimposed on skulls may provide insight into the biological significance of skull and brain morphology. The high degree of cranial flexion and the globose brain shape of Daubentonia appear to be related to the functional demands of its gnawing mechanism. The broad frontal lobes of indriids are correlated with orbital orientation; differences in frontal lobe sulcal pattern suggest greater elaboration of the motor filed for the hand in indriids than in lemurids. Several features of lorisid cranial anatomy are discussed. It is suggested that, as a first approximation, increased splanchnocranial declination in small prosimians results from the necessity of accommodating relatively large eyes in a skull with a relatively small splanchnocranium.
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  • 59
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    Journal of Morphology 124 (1968) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 60
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    Journal of Morphology 124 (1968), S. 283-293 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In March through April when the oocyte growth in the ovaries of the wall lizard (Hemidactylus) is very rapid, the yolk nucleus continues to persist through various stages of previtellogenesis. This persisting yolk nucleus and associated cell components have been studied with histochemical techniques. The spherical and dense yolk nucleus stains for protein, lipoprotein and RNA. It does not form any close morphological association with the other cell components such as the mitochondria, lipid bodies (L2), spaces or canals, diffuse sudanophilic substance and dense bodies, which are arranged into three zones round the yolk nucleus proper. The mitochondria stain for lipoprotein; the L2 bodies consist of phospholipid; the spaces do not contain any material demonstrable with histochemical techniques; and the ooplasm containing the diffuse sudanophilic substance and dense bodies shows lipoprotein, protein and RNA. Eventually, the yolk nucleus disintegrates, and its substance as well as the other cell components are distributed in the cortical ooplasm of oocytes which are ready to form the yolk bodies.Concepts of the origin, morphology, cytochemistry and function of the yolk nucleus in the oocytes of invertebrates and vertebrates, which have come about recently through the application of cytochemical and submicroscopical techniques, are discussed.
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  • 61
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    Journal of Morphology 124 (1968), S. 353-359 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Symmetrical gaits of 37 breeds of dogs were analyzed. Usual walking and trotting gaits resemble those of other carnivores of similar size and conformation. Only certain long-legged dogs pace - usually at the fast walk or slow run. At the moderate walk, long-legged dogs tend to use lateral-couplets gaits, whereas short-legged breeds tend to use single-foot gaits. Many dogs must turn the axis of the body slightly from the line of travel at the trot to prevent interference between fore and hind feet. The relative duration with the ground made by fore and hind feet is discussed, usual support-sequences of the varicus gaits are presented, and the amount of variation is shown.
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  • 62
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    Journal of Morphology 124 (1968), S. 387-421 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Electron microscope autoradiography was used to study cartilage from regenerating limbs of adult newts, Triturus, after intraperitoneal injections of proline-3H. The labeling in the endoplasmic reticulum, small vesicles, Golgi vacuoles, ground cytoplasm and extracellular matrix was compared during the secretion of radioactive products. The data appear to indicate that a large part of the radioactive secretion probably leaves the cell after having been in only one cellular compartment. Although this compartment may be the endoplasmic reticulum, a considerable amount of radioactivity fluxes through the ground cytoplasm and the possibility cannot be excluded that some secretory components leave the cell directly from the ground cytoplasm. The data appear incompatible with the hypothesis that all the radioactivity seen in the extracellular matrix arrived there via a single pathway involving first the endoplasmic reticulum and then the Golgi vacuoles. It is not, however, incompatible with a hypothesis that a fraction of the radioactive product uses this pathway.
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  • 63
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    Journal of Morphology 124 (1968), S. 249-261 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An electron microscope examination of the minute bristle organules in the Drosophila eye revealed an organisation characteristic of insect hair sensilla. They were derived from four concentrically arranged cells which were active at the mid-pupal stage in producing the bristle, socket and receptor structures. Two of the cells degenerated towards the end of pupation, the mature organule consisting of a bipolar sense cell and an accessory cell.Growth in length of the bristle was accompanied by a proliferation of longitudinally oriented microtubules which gradually disappeared after maximum growth had been achieved. The breakdown of microfibrillar aggregates, which were also present as transient structures in the developing bristle and showed some correspondence with the longitudinal ridges formed at the surface, may similarly be related to the establishment of cell shape by the deposition of the cuticle which occurred at the same time.
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  • 64
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    Journal of Morphology 124 (1968), S. 313-320 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Forty-two unselected ovaries from adult mares were examined histologically, and with histochemical methods for mucins. A considerable part of the surface of the ovulation fossa was directly covered by columnar epithelium, with many ciliated cells. This epithelium, which was distributed mainly on the anterior side of the ovulation fossa, closely resembled the contiguous epithelium of the infundibulum of the oviduct, was frequently folded, and gave rise to short clefts projecting into the ovarian substance. The remainder of the ovulation fossa was covered by non-ciliated, low cuboidal or squamous epithelium, lacking folds or clefts.“Fossa cysts,” up to 6.5 mm in diameter, were observed in the ovarian tissue around the ovulation fossa in 27 (64%) of these ovaries. Both simple and branched, tubular and vesicular forms were present, and all were blind-ending. Their epithelial lining cells, which varied from simple squamous to columnar in type, were frequently ciliated. Many fossa cysts contained secretions histochemically similar to those of the columnar epithelium of the ovulation fossa and infundibulum. Both sialic acidcontaining and neutral mucins were present. It is suggested that these cysts were probably derived by ingrowth from the columnar epithelium of the ovulation fossa. This epithelium may be of müllerian duct origin.
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  • 65
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    Journal of Morphology 124 (1968) 
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  • 66
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    Notes: Light microscopic sections of the adult opossum (Didelphis virginiana) spleen were observed to lack venous sinuses; this primitive mammalian spleen may be classified as non-sinusal in nature. In the spleen of the opossum, the capillary segments of the penicillar arteries lacked ellipsoid sheaths characteristic of certain mammalian spleens.Separating the lymphoid nodules from the surrounding red pulp was a distinct band of vascular tissue, the marginal zone. Arising from the central artery within the lymphoid nodule, vessels of capillary dimension were observed to terminate within the marginal zone and the area between lymphoid nodule and marginal zone. In addition to the vascular channels established by the terminal arterial vessels within the red pulp, the system of vessels within the marginal zone has been implicated as an important intermediate vascular channel within the spleen.
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  • 67
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    Notes: A total of 54 embryos of Chrysemys (Chelonia) and 29 embryos of Aristelliger (Lacertilia) were used in examining septation of the embryonic bulbus cordis. Division of this region of the heart includes a period of cushion or septal primordia formation and a period of physical partitioning. In both reptilian genera, the physical configuration of the early bulbus, the temporal sequence of appearance of the endocardial cushions, the number of major endocardial cushions, the primordia composing the two primary bulbar septa, and the mode of descent of the bulbar septa are strikingly similar. The two genera differ primarily in the pattern of the endocardial cushions and consequently the rotation of the two bulbar septa. In both the turtle and the lizard the aortico-pulmonary septum passes through an angle of about 120° in its descent toward the ventricle. In Aristelliger the aortic septum rotates through an angle of approximately 120°. By contrast, the same partition in Chrysemys spirals through an angle of about 90°. The lesser spiral of this septum in the turtle is interpreted as the result of a decrease in the rotation of distal endocardial ridge 4. The pattern of the two bulbar septa in the turtle appears to represent an advanced phylogenetic feature in terms of the evolution of the reptilian bulbus cordis.
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  • 68
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    Journal of Morphology 125 (1968), S. 71-103 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Oogenesis and the relationships between oocytes and other ovarian tissues have been studied in Sypharochiton septentriones. The ovarian tissues were examined by electron microscopy and by histochemical methods.The sac-like ovary is dorsal, below the aorta, and opens to the exterior by two posterior oviducts. Ventrally, the ovarian epithelium is folded inwards to form a series of plates of tissue, which support the developing ova. Each ovum is attached to a tissue plate by a stalk, the plasma membrane of which is bathed by the blood in the tissue plate sinus. Dorsally, ciliated vessels from the aorta enter the ovary and open into blood sinuses in the top of the plates.After each germinal epithelial cell rounds up to become a primary oogonium, it undergoes four mitotic divisions to give rise to a cluster of 16 secondary oogonia. Of these, the outer ones become follicle cells and the inner ones become oocytes. As in other molluses, the increases in nuclear and nucleolar volume are relatively greatest towards the end of previtellogenesis, when chromosomal and nucleolar activity are most intense. This phase of activity is accompanied by a great increase in cytoplasmic basophilia. Subsequently this basophilia is decreased during vitellogenesis, when chromosomal and nucleolar activity diminish. Fluid filled interstices appear in the cytoplasm during early vitellogenesis. Protein yolk deposition is associated with these interstices, but the lipid yolk appears to arise de novo. The follicle cells do not appear to be directly involved in oocyte nutrition.At times during oogenesis, certain manifestations of polarity can be found in the oocyte. This polarity is based on an apical-basal axis and can be related to the nutritive source of the oocyte, namely the blood which bathes the plasma membrane of the oocyte in the stalk.Numerous granulated cells are present in the ovarian tissue plates and ventral epithelium as storage cells containing lysosomes, and they are capable of phagocytosis and micropinocytosis of extracellular material. A scheme is outlined whereby reserves in these cells may be incorporated into the oocyte cytoplasm. Lysosomal activity is responsible for autolysis of the cells as well as resorption of unspawned ova.
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  • 69
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    Notes: The postnatal development of the pelage and ventral gland of male Mongolian gerbils ranging from newborn to 86 days of age was studied. The development of the gerbil pelage follows a pattern similar to that observed for other rodents. The length of the dorsal and ventral skin juvenile hair cycle was found to be 26 to 28 days with a 15 to 18 day anagen and a ten to 11 day catagen and telogen. Hair follicles in the ventral gland began growth ten days later than those of the general pelage and secondary follicles budded from the sides of primary follicles. The ventral gland area differed from the general pelage in that it lacked a panniculus carnosus. The ventral gland is a complex of pilosebaceous glands which, in the adult, fill the entire hypodermis. The length and width of the pilosebaceous canals of the gland units are greater than those of the dorsum. The period of telogen of the hair follicles in the ventral gland is very short. The mid-ventral gland of the male gerbil appears to be a secondary sexual characteristic.
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  • 70
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    Journal of Morphology 125 (1968) 
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  • 71
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    Notes: The ultrastructure of lymphatic capillaries in the tail fin of Rana catesbiana larvae was investigated. With the use of a colloidal marker particle (Biological Carbon) the extent that these delicate vessels ramify throughout the fin region was demonstrated. This opaque substance also serves as a marker particle for identification of lymphatics with some degree of certainty at both light and electron microscopic levels. The cytoplasm of the lymphatic endothelial cell is abruptly attenuated beyond the perinuclear region, reaching widths as thin as 300 Å. Lymphatic Anchoring filaments are present, but to a lesser degree than noted for other species studied. Other features of interest include an extensive Golgi complex and electron dense bodies that are surrounded by a smooth surfaced unit membrane. These bodies are somewhat heterogeneous in size (500 Å up to 0.5 μ in diameter) and density. Numerous exit channels are provided by the extensive supply of lymphatics throughout the tail fin region of amphibian larva thus allowing them to serve an important function during metamorphosis. It is suggested that these vessels also act as passageways through which lysed cellular and connective tissue components may be rapidly removed during the process of tail fin resorption.
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  • 72
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    Notes: The cuticle of Watersipora nigra is at first translucent, but it later becomes black and differentiates into two layers. It is composed, at least in part, of a protein-polysaccharide complex. Calcified parts are three-layered: (1) an outer, cuticular layer, (2) a calcium carbonate skeleton deposited on a matrix of acid mucopolysaccharide, and (3) a “skeletal membrane.” The relationships of these layers indicate that the skeleton is intracuticular. A layer of cuticular material, the “intercalary cuticle” is present in lateral walls, but not transverse walls; it may become calcified in some species. The cuticles of calcified and uncalcified parts of cheilostomes are not necessarily homologous.
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  • 73
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    Journal of Morphology 126 (1968), S. 67-93 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cell population and neuropile morphology of larval and adult brains of the monarch butterfly, Danaus plexippus plexippus, L., are compared. The larval brain is in continuous transition, the processes of adult brain development being underway from the earliest larval stages. It is characterized by a less diverse population of cells and more homogenous fiber areas than those of the adult. Neuroblasts, which divide to form the neurones of the adult brain, occur either in discrete proliferation centers or scattered among the larval ganglion cells. The larval brain contains, in addition to small homogeneous antennal centers and a distinct larval optic center, rapidly developing adult optic centers, corpora pedunculata, and protocerebral bridge. The larval brain lacks a central body. Major differences between larval and adult brains are clearly related to the increased dependence of the adult upon sensory input from the eyes and antennae.
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  • 74
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    Journal of Morphology 126 (1968), S. 123-161 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Distomus variolosus from Roscoff, France, comprises two sorts, differing in their branchial and gonadal patterns. Their sexual cycle has been followed histologically and micro-anatomically. Gonads begin as clumps of lymphocytes that persist along germinal tracts. Cavitation of the clump, growth of the gonoduct, maturation of the gametes, and elaboration of accessory structures are described. Oocytes develop in a linear series in each ovary; only one or two reach maturity in each gonad. The released egg and subsequent tadpole may be held to the ovary by a “leash” formed of the partially everted outer follicle of the egg. Post-mature gonads deteriorate. Testes disrupt altogether; ovaries may persist as moribund loci of remnant germinal tissue. The sharp right-left hermaphroditism of the zooid appears to combine with a subtler anterior-posterior gradient of sexual determination.
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  • 75
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    Notes: In a hydrozoan jellyfish, the female gonad is differentiated from a specialized region of the epidermis near the manubrium. Changes in the oocytes during growth and vitellogenesis are described as observed with electron microscopic and cytochemical techniques. Three major types of yolk are formed; these include lipid, glycogen, and membrane-bound granules consisting of both protein and carbohydrate. The latter first appear evident within vesicular and cisternal elements of the numerous Golgi complexes. The orientation and structural variations noted between the endoplasmic reticulum and forming face of the Golgi complexes suggest that the protein component of the yolk granules may be transferred from the cisternae of the endoplasmic reticulum to the Golgi complex where it is joined to carbohydrate perhaps synthesized by the Golgi complexes. Stages in the release of the precursor yolk material sequestered in cisternal elements of the Golgi complexes are illustrated. The presence of coated and uncoated vesicles in the Golgi regions and their possible role in intracellular transport are described and discussed. The presence and possible method of morphogenesis of vesiculate yolk bodies are also described. What appear to represent invaginations of the oolemma extend into the ooplasm and display a special orientation with respect to lamellae of the rough-surfaced endoplasmic reticulum. Intraooplasmic synthesis appears to constitute the major pathway for protein-carbohydrate yolk deposition.
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The dipnoan heart is only in part structurally developed to support a separated circulation in pulmonary and systemic circuits. In the present investigation biplane angiocardiography has been used to describe the extent of such a double circulation and the factors which may modify it in the African lungfish, Protopterus aethiopicus.Contrast injections in the pulmonary vein revealed a clear tendency for aerated blood returing from the lungs to be selectively dispatched to the anterior branchial arteries giving rise to the major systemic circulation. Contrast injections in the vena cava delineated the sinus venosus as a large receiving chamber for systemic venous blood. Contraction of the sinus venosus discharged blood into the right, posterior part of the partially divided atrial space. Contrast injection in the pulmonary vein showed that vessel to pass obliquely from right to left such that blood was emptied distinctly into the left side of the atrium. During contraction the atrial space tended to retain a residual volume in its anterior undivided part which minized mixing.Ventricular filling occurred through separate right and left atrio-ventricular connections. Right-left separation in most of the ventricle was maintained by the partial ventricular septum, the trabeculated, spongelike myocardium and the mode of inflow from the atria. Mixing in the anterior undivided portion of the ventricle during the ejection phase was slight due to a streamlined ejection pattern.The outflow through the bulbus cordis occurred in discrete streams which in part were structurally separated by well developed spiral folds. In the anterior bulbus segment the spiral folds are fused and make completely separate dorsal and ventral outflow tracts. The ventral bulbus channel provides blood to the three anterior branchial arteries. The second and third branchial arteries are large and represent direct shunts to the dorsal aorta. The fourth and fifth branchial arteries are gill bearing and receive blood form the dorsal bulbus channel. The most posterior epibranchial vessels give rise to the pulmonary arteries.
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  • 77
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Sinistral ovariectomy in the Japanese quail resulted in some hypertrophy of the rudimentary right gonad in about 80% of the cases. The hypertrophied right gonads were composed of cords of epithelial origin, fat laden cells and a connective tissue stroma containing masses of lymphocytes. Neither cortical tissue nor germ cells were found in any of the gonads. In some cases regeneration of a testis-like tissue was seen on the site of removed left ovary. This, however, did not alter the effects of ovariectomy on rudimentary right gonad, accessory sex organs, plumage or sexual behavior. Neither Wolffian nor Müllerian ducts exhibited hormonal stimulation in poulards showing hypertrophy of right gonad with exception of the latter in two poulards. Early orchiectomy inhibited growth and differentiation of the cloacal gland. This organ revealed no noticeable stimulation in poulards showing hypertrophied right gonads. Castration produced no significant changes in plumage of males. Similarly, sinistral ovariectomy did not effect the first juvenile, but the second juvenile, adult winter and summar plumages changed to the male type. However, the plumage of some of these poulards began to revert to the female character as early as 10 to 12 weeks following ovariectomy. The behavior of capons and poulards revealed no conspicuous difference and neither showed any masculine behavior. The average weight of adult females was 20 to 30 gm above that of adult males whereas that of capons was above normal males and that of poulards below normal females. The average weight of capons was somewhat above that of poulards.
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  • 78
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    Journal of Morphology 125 (1968), S. 281-301 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Lymphatic tissues of inbred lines of White Leghorn chickens and 20-day embryos (inbreeding coefficient exceeds 95%) were used in the experiments, e.g. line 6 was susceptible, line 7 was resistant, and line 15 I was intermediate in response to the virus. Enzyme reactions were studied in cryostat-cut sections of tissues and in tissue minces by colorimetric procedures. Numbers of isozymes and proteins of lymphatic tissues were resolved by disc gel electrophoresis.Colorimetric tests showed that intensities of lactate, malate, isocitrate and succinic dehydrogenase catalyzed reactions were higher in the bursae of 15 I 20-day embryos than they were in bursae of either line 6 or 7 embryos. Intensity of dehydrogenase reactions of the spleen (15 I embryos) exceeded that found in line 6 and 7 embryos. Intensity of diaphorase reactions in the spleen and thymus was fairly uniform in all lines of embryos. Intensity of DPN diaphorase reactions in the bursae of line 15 I embryos exceeds that found in either line 6 or 7 embryos. Intensity of enzyme reactions leveled off to become fairly uniform in lymphatic tissues of chickens 3-4 weeks post hatching with the exception that dehydrogenase reactions were less intense in the thymus of 15 I chickens.Photodensitometer scans of acrylamide gel columns showed that proteins of line 6 lymphatic tissues combined with less Amido black 10B than lymphatic proteins of either line 15 I or 7 embryos. There was fairly good agreement between concentrations of strong mobility (components 1-9) and weak mobility (components 10-16) in lymphatic tissues of all lines of embryos with the exception that strong mobility proteins were about twice as concentrated in line 15 I bursae. Variable numbers of lactate isozymes were found in the lymphatic tissues of 20-day embryos.
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  • 79
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    Journal of Morphology 125 (1968), S. 367-377 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphology of the gonads and epididymides, and an analysis of selected scale characters of six hybrid males presumed to be the result of the natural mating of the all-female whiptail lizard species Cnemidophorus neomexicanus and the bisxual species C. inornatus are presented. The histological appearance of the gonads and epididymides reveals a seasonal cyclicity with respect to the production and storage of spermatogenic elements. On the basis of the limited sample, it is postulated that some abnormality may be present in gametogenesis of these hybrid males which could be related to their chromosomal number. The data on scale characters support the interpretation that these hybrids are intermediate with respect to the parental species.
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  • 80
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In 85 frogs and 29 rats, the entire gastrocnemius muscle was removed. After removal of as much connective tissue as possible, about two-thirds of the remaining muscle was finely minced with a scissors. These minced fragments were orthotopically re-implanted, and the overlying skin was sutured. As the implanted muscle fragments degenerate, new muscle fibers appears in the regenerate. The proportion of connective tissue to muscle is usually greater than normal especially in the frog. Grossly, normal relationships are established with the cut ends of the Achilles and proximal tendons, as well as with the blood vessels and nerves. The total diameter of the regenerated muscle is almost always less than half of that seen in normal muscles. Regeneration of muscle is much more extensive in the rat than in the frog, and it occurs almost twice as rapidly. The histology of the regenerative process is described for both the frog and the rat.
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  • 81
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Presumptive myoblasts from the regenerating tail of the lizard plated at clonal density undergo extensive growth giving rise to large colonies. After several days in culture at 31°C some of the cells begin to round up and assume a spherical morphology. The number of rounded up cells increases over the next few days until 50 to 75% of the cells are rounded up. If the cultures are switched to a permissive medium, fusion and differentiation occur. If they are left in a nonpermissive medium fusion does not occur. Instead, the cells stretch out on the substrate and become long and attenuated or broad and strap-like. However, differentiation may continue in some of the cells giving mononucleate cells with cross striations. Autoradiographic studies indicate that the rounded up cells represent a post-synthesis, prefusion population of cells.
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  • 82
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    Journal of Morphology 126 (1968), S. 107-122 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cervicothoracic musculature of the adult cockroach, Nauphoeta cinerea (Olivier) is described for the first time. The adult thoracic ventral intersegmental muscles are compared with those of the nymph and of the adult cockroach, Periplaneta americana (Linnaeus).
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  • 83
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    Journal of Morphology 126 (1968), S. 199-210 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The thoracic skeleton and musculature of the adult bittacid mecopteron Bittacus strigosus Hagen is described. In its musculature, Bittacus shows only moderate differences from two panorpids (Neopanorpa, Panorpa) that have been studied by Maki ('38) and by Hasken ('39), respectively. Not only are these three genera much alike in their musculature generally, but in all of them, and in Boreus (Boreidae) too, the mesothorax is extremely similar to the metathorax. Functional emphasis (for flight) on either of the two pterothoracic segments has not appeared among neuropteroid insects at the metopteran evolutionary level.Although the “snowfleas” of the genus Boreus possess striking alterations of pterothoracic structure in comparison with other mecopterons (Füller, '54, '55), these are related to their unusual activities and have not, to any great extent, affected the two pterothoracic segments differentially.In terms of thoracic specialization, the overall mecopteran pattern represents a stage somewhat advanced beyond the primitive conditions exemplified by the Megaloptera and certain coleopterous larvae, but one that is in general less highly developed than is charatceristic of such neuropteroid orders as the Siphonaptera, Diptera, Trichoptera, and Lepidoptera.
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  • 84
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The comparative morphology of the gonads and fat bodies of members of 17 genera and 46 species of caecilians (Amphibia: Gymnophiona) is described and analyzed. Comparison is made with the morphology of salamanders and frogs in order to elucidate evolutionary trends and relationships within the order Gymnophiona and within the class Amphibia. The structure of the testis lobes and transverse and longitudinal ducts is described based on gross dissection and histological investigation. The pattern of spermatogenesis and interstitial tissue changes are described and compared with those of other amphibians. A trend toward fusion of testis lobes is analyzed. The characteristics of the seasonal reproductive cycle of male Gymnopis m. proxima are described, and evidence for cyclic reproductive activity in other forms is presented. The morphology of the ovaries and ova is described. Size of ovary and size and number of ova is dependent on the state of maturation of the ova. Some evidence for seasonal ovum production and breeding is presented. Fat body morphology is found to be correlated with size, nutrition, and gonad condition, as in other amphibians.
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  • 85
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    Journal of Morphology 126 (1968) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 86
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The distribution of carbohydrates, proteins, and lipids was studied in the cochleae of kangaroo rat, gerbil, and guinea pig using both fixed paraffin sections and fresh-frozen cryostat sections. Enzyme distribution in the cochleae of the three speices was studied with both EDTA-decalcified and undecalcified fresh-frozen cryostat sections.Although the cochleae of the three species are morphologically different, their distributions of proteins, carbohydrates, and lipids are similar. The zona pectinata of the basilar membrane - which is hypertrophied in the kangaroo rat and gerbil but normal in the guinea pig - stains the same in all three species. The unique, flaskshaped Hensen cells of the kangaroo rat contain more protein than do the normal Hensen cells of the gerbil and guinea pig. At least some of the protein in the kangaroo rat Hensen cells is in the form of carboxylic esterases which are not affected by 10-4 M eserine, but are inhibited by 10-2 M eserine and 10-6 M E600. More than one population of carboxylic esterases is indicated by this reaction to inhibitors and by the results of enzyme distribution tests which used different substrates. A high concentration of malate dehydrogenase in the kangaroo rat Hensen cells may be related to the synthesis of carboxylic esterases. The possible role of these esterases in cochlear functioning is discussed.
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  • 87
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This report presents light microscopic descriptions of normal histology, including innervation, of the lymph glands and jugular bodies, of larval and adult Rana catesbeiana. A brief description of two other adult organs, the propericardial and procoracoid bodies, is also included. The parenchyma was studied by employing the May-Grunwald-Giemsa staining technique for better cytoplasmic differentiation; the Periodic Acid-Schiff technique and hematoxylin and eosin yielded clearer nuclear and cytoplasmic delineations. The intercellular portion of the stroma was studied from sections stained with Masson's trichrome, Weigert's elastic stain, Periodic Acid-Schiff and Wilder's reticulum stain. Demonstration of phagocytes was facilitated by intraperitoneal India ink injections followed by the above staining procedures. Innerrvation was observed in serial sections of silver impregnated whole organs as well as in the other serial sections. These organs are lymphocytopoietic and to a certain extent granulopoietic; they also serve, like the spleen, as graveyards for dead cells and most probably play a role in immunity especially in the synthesis of antibodies as indicated by the presence of plasma cells, macrophages and lymphocytes.
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  • 88
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    Journal of Morphology 121 (1967) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 89
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Different types of sebaceous glands in guinea pigs were chosen to study their comparative responsiveness to steroid hormones. Glands selected were (1) Sebaceous glands associated with rudimentary hair in the supracaudal gland; (2) Free sebaceous glands of the nipple; (3) Sebaceous glands associated with the hair. The results showed distinct differences in sensitivity among these different sebaceous glands and according to the sex of the experimental animal. The most responsive of the glands in males was the supracaudal gland; in the female the most responsive glands were the sebaceous glands of the nipple. Sebaceous glands associated with normal hair were relatively insensitive to changes in hormonal level. In all cases testosterone propionate was more potent in stimulating any of the sebaceous glands than progesterone. After gonadectomy, various sebaceous glands showed unequal states of depletion due to the different rates of differentiation and disintegration of their cells into sebum.
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  • 90
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The lymphoid cell population of thymus in the rattlesnake and king snake is similar to that of mammals. Lymphocytes occupy the interstices of an epithelial cell framework. An abudance of tonofilaments and desmosomes occupy the cytoplasm of epithelial cells with light, homogeneous nuclei and prominent nucleoli. Other epithelial cells contain phagocytized material in a dense cytoplasm which surrounds an irregular nucleus with heavily clumped chromatin. Small, granular vesicles are found within some epithelial cells.Myoid cells occur in the medullary area. In mature forms, myofibrils are arranged in a concentric fashion around the nucleus and occupy much of the cytoplasmic volume. The presence of developmental stages of these cells suggests their differentiation within the thymus of the adult animal.
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  • 91
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    Journal of Morphology 122 (1967) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 92
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    Journal of Morphology 122 (1967), S. 19-33 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ovary in Callosobruchus analis consists of telotrophic ovarioles with the so called nurse cells confined to one chamber at the anterior end of the ovariole. There are three types of lipids in the ovary: (1) L1 bodies that are present in the early oocytes, in the posterior prefollicular tissue and in the follicular epithelium and contain unsaturated phospholipids; (2) L2 bodies that have a complete or incomplete sheath of phospholipids and a triglyceride core; (3) L3 bodies that are formed of highly saturated triglycerides. Lipids are absent from the trophic tissue. In a mature oocyte the L1 and L2 bodies are cortical in distribution while the L3 bodies are centrally located.The mitochondria contain lipoproteins with RNA. The yolk spheres are acid mucopolysaccharides and protein in nature. The precursors of the yolk spheres appear first in the cortical coplasm and are absent from the follicular epithelium or the trophic tissue. The nucleolus of the oocyte shows evidence of extrusions that are believed to pass into the ooplasm.There are no nutritive cords connecting the trophic tissue to the oocytes; nor is there any evidence of any histochemically demonstrable nutritive material being contributed to the oocyte by the trophic tissue. The circumstantial evidence points towards a contribution of the raw materials to the oocyte by the haemolymph either through or in between the follicular epithelium in some soluble form or as submicroscopic particles.
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  • 93
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Parenteral administration of methylcellulose causes massive splenomegaly and hemolytic anemia in rats. The red pulp of the spleen is markedly cellular due mainly to: (1) large numbers of voluminous free macrophages containing methylcellulose-induced vacuoles, (2) an increase in the number of plasma cells and (3) stasis of blood evidenced by a large number of erythrocytes and platelets in vessels, sinuses and cords. White pulp changes are usually less marked. Here the major change is the presence of macrophages containing methylcellulose-induced inclusions. The slow circulatory time in the spleen and the increase in macrophages may cause the hemolytic anemia observed in these animals.
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  • 94
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The squirrel monkey uterine cervix was studied macroscopically and microscopically in intact and ovariectomized monkeys. The effect in ovariectomized monkeys of estradiol dipropionate or progesterone of both given after estrogen priming was studied by PAS staining.The lower portion of the cervix was dilated to form a vestibule into which projected fibromuscular colliculi which arose from the isthmic end of the cervix. The stratified squamous epithelium of the vagina was continuous through the external os with a similar epithelium lining the vestibule and covering the external surfaces of the colliculi. The transitional zone between the stratified epithelium and columnar cells was variable. The colliculi were covered internally with mucosal folds of columnar epithelium continuous with those of the endocervical canal.Glycogen concentration in the smooth muscle did not fluctuate markedly, irrespective of the hormones used. Glycogen granules were more numerous in the stratified squamous epithelium. Malt-diastase-resistant material appeared to be more abundant in the columnar epithelium and glandular lumina when the monkeys received both hormones than when they received solely estrogen or progesterone.
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  • 95
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    Journal of Morphology 122 (1967) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 96
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    Journal of Morphology 122 (1967), S. 169-173 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Histological examination of the fibrous and cellular connective tissue components of the periodontal ligament in the Caiman and the Alligator reveals the presence of reticular fibrillae, collagenic, elastic, and oxytalan fibers, as well as fibrocytes, osteoblasts, cementoblasts and epithelial rests. The oxytalan fibers differentiated by the peracetic acid aldehyde-fuchsin method are most numerous in the coronal region, radiating from the primary cementum into the periodontal ligament a short distance. Oxytalan fibers in fewer numbers are found interspersed between the oblique and the horizontal principal fiber bundles. Inasmuch as the crocodilian teeth have continuous replacement and thus a relatively short functional life, the oxytalan fibers of the Caiman and the Alligator appear to be proportionally fewer in number than they are in the mammalian periodontal tissues. The presence of the oxytalan fibers and epithelial rests in the Order Crocodilia (Crocodilia) adds to the number of dental structures shared with the Class Mammalia (Mammalia) (mammals) such as a stellate reticulum, a primary and secondary cementum and a periodontal ligament. This furnishes additional histological evidence for evaluation of the phylogenetic position of this group.
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  • 97
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Histochemical methods, especially azo dye methods for detecting acid phosphomonoesterase activity were applied to normal, regenerating and denervated, amputated limbs from larval Amblystoma maculatum. Efforts were made to control inactivation of enzymic activity and diffusion of both enzyme and reaction product. “Base-line” values for enzymic activity were determined for normal limbs. Activity appeared most intense in macrophages, less intense in epidermis and cartilage matrix. Some activity was detected in Schwann cells, peri- and endoneurium and muscle fibers form normal limbs. Enzymic activity in regenerating limbs was strongest within macrophages which appeared in increased numbers especially in early stages. Wound tissue showed little increased activity. As the blastema formed, increased enzymic activity was detected in epidermis and within increased numbers of macrophages. Chondrocytes showed increased activity especially during cartilage matrix deposition. Amputated, denervated limbs showed large numbers of active macrophages beneath and within epidermis and along muscle. As regression commenced, areas of cartilage matrix breakdown showed increased enzymic activity but, in general, greatest activity was in macrophages. The various possible roles of acid phosphomonoesterase activity in the specific biological situations dealt with are considered in light of such observations.
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  • 98
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    Journal of Morphology 122 (1967), S. 249-263 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Adult newts, Triturus viridescens, were treated with from 1.0-10.0 μg/g body weight of actinomycin D one day before amputation of both forelimbs. Mean survival times ranged from over 50 days in newts treated with 1.0 μg/g to 13.2 days in animals given 10.0 μg/g body weight of actinomycin. Low doses little altered the course of regeneration, but animals treated with over 2.0 μg/g never formed blastemas. In another series, animals were given doses of 2.5 μg/g body weight of actinomycin D at intervals from 14 days before to 30 days after amputation. It was found that certain signs of toxicity (loss of equilibrium) are related to the time of administration of the drug whereas others (hemorrhage into the limb stumps) are restricted to a definite phase of the regenerative process. Early administration of actinomycin completely inhibits regeneration whereas later treatment results in a considerably lessened effect. The postamputational stages which are basically destructive in nature are not noticeably affected by actinomycin D, but the phases of dedifferentiation, blastema formation and redifferentiation are strongly inhibited.
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  • 99
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    Journal of Morphology 122 (1967), S. 345-365 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The major events associated with the morphogenesis of the amphibian alimentary tract are described and illustrated with a series of photomicrographs that present a continuous account of the differentiation process from its onset at stage 38 until the initiation of feeding at stage 46. Histological evidence is presented for the normal disappearance of the midgut region of the archenteron and the de novo formation of the intestine through the yolk mass. The mechanics of intestinal lumen formation are discussed in terms of the dynamic autonomous actions and interactions of the endoderm and the splanchnic mesoderm. The opening of the intestinal lumen as a consequence of cytolysis or cellular digestion is discounted. The relation of the present observations to the previously described polar endoderm cells is considered.
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  • 100
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    Journal of Morphology 123 (1967) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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