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  • 1
    Electronic Resource
    Electronic Resource
    Nerve growth factor in skeletal tissues of the embryonic chick (1990)
    Springer
    Cell & tissue research 260 (1990), S. 507-511 
    Details
    ISSN: 1432-0878
    Keywords: Nerve growth factor ; Osteogenesis ; Chick embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study demonstrates, via immunohistochemistry and bioassay, the presence of NGF in embryonic bone and cartilage of the chick. Embryos were killed on days 6–9 of incubation at 12 h intervals, and on days 10–18 at 24 h intervals. Paraffin-embedded sections of hind limbs or buds were immunostained with a polyclonal antibody against NGF and the biotin-avidin-horseradish peroxidase technique. Immunostaining was positive in both bone and cartilage, with cartilage staining more intensely. For bioassay, bones from the hind limbs of 9- and 12-day embryos were fast-frozen, lyophilized, and homogenized with Medium 199 (M199). Dorsal root ganglia from 8-day embryos were cultured for 24–36 h with rooster plasma, M199, and varying concentrations of bone homogenate. Significant neurite outgrowth was seen, with the greatest response elicited by 12-day bone homogenate. Addition of anti-NGF to the cultures abolished neurite outgrowth. The results indicate that NGF is present in cartilage and bone of the chick embryo; it may determine the density of sympathetic innervation to the developing skeletal tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297230
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  • 2
    Electronic Resource
    Electronic Resource
    The postnatal development of the pelage and ventral gland of the male gerbilThis investigation was supported in part by National Science Foundation grant 05679-248. (1968)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 125 (1968), S. 303-313 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The postnatal development of the pelage and ventral gland of male Mongolian gerbils ranging from newborn to 86 days of age was studied. The development of the gerbil pelage follows a pattern similar to that observed for other rodents. The length of the dorsal and ventral skin juvenile hair cycle was found to be 26 to 28 days with a 15 to 18 day anagen and a ten to 11 day catagen and telogen. Hair follicles in the ventral gland began growth ten days later than those of the general pelage and secondary follicles budded from the sides of primary follicles. The ventral gland area differed from the general pelage in that it lacked a panniculus carnosus. The ventral gland is a complex of pilosebaceous glands which, in the adult, fill the entire hypodermis. The length and width of the pilosebaceous canals of the gland units are greater than those of the dorsum. The period of telogen of the hair follicles in the ventral gland is very short. The mid-ventral gland of the male gerbil appears to be a secondary sexual characteristic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051250304
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  • 3
    Electronic Resource
    Electronic Resource
    The effects of cold-stress, hibernation, and prolonged inactivity on bone dynamics in the golden hamster, Mesocricetus auratus (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 167 (1981), S. 43-51 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of cold-stress and hibernation on bone dynamics in the femurs of hamsters were investigated using histometric analyses. Control animals were maintained at 27° C for 90 days; experimental animals were kept at 5° C and hibernated for 7, 15, 21, 50, or 90 days. Histometric analyses of cross sections indicated that bone diameter and cortical thickness at the femoral midshaft increased after 83 days of extreme cold and 7 days of hibernation but decreased significantly after 69 days of cold stress and 21 days of hibernation. Osteoporosis was evident although the number of osteons per unit area of bone increased during hibernation. An initial decrease in the number of non-Haversian longitudinal vessels per unit area of bone was seen in experimental animals which was apparently related to a corresponding reduction in cortical thickness. Lacunar area increased in these animals, suggesting that osteocytic osteolysis may be a significant mechanism for calcium regulation during hibernation.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051670105
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  • 4
    Electronic Resource
    Electronic Resource
    Submandibular duct salivary bladder of the rat (1987)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 192 (1987), S. 247-256 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This investigation is a light and electron microscopic description of the submandibular duct salivary bladder of the rat, a dilation of the distal end of the main excretory duct. The wall of the bladder consists of (1) a mucosa with pseudostratified epithelium, (2) a submucosal layer of connective tissue, and (3) an underlying layer of striated muscle. The pseudostratified columnar epithelium lining the bladder is composed of three cell types: (1) light cells, (2) dark cells, and (3) basal cells. The lamina propria contains bundles of collagen, attenuated fibrocytes, capillaries with fenestrated endothelia, and nerve fibers which enter the epithelial layer. The capillaries of the submucosa are not fenestrated. The morphology of the wall of this structure provides evidence that the primary fluid of the submandibular gland is modified in the bladder by transepithelial fluid and ion transport.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051920307
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  • 5
    Electronic Resource
    Electronic Resource
    Salivary glands of heteromyid rodents, with a summary of the literature on rodent submandibular gland morphologyThis investigation was supported in part by U.S.P.H.S.-N.I.D.R. grant DE-02315 to Robert Gerstner and in part by NIH General Research Support grant FR 5332-08 to Ormond G. Mitchell. (1970)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 179-193 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The principal parenchymal elements of the submandibular glands of the heteromyid rodents Dipodomys merriami, Perognathus longimembris, Perognathus fallax, Perognathus penicillatus and Perognathus baileyi consist of acini, granular tubules and striated ducts.Acinar cells of the four species of Perognathus are aniline blue, PAS (magenta) and Alcian blue (pH 2.5) positive and metachromatic with toluidine blue and safranin. The granules of the tubule cells are orthochromatic and react with aniline blue, orange G, the PAS reagent (deep pink) and the tryptophan indicator, xanthydrol. Acinar and tubule cells of D. merriami exhibit similar reactions except for the Alcian blue stain. Acinar cells of D. merriami do not react with Alcian blue.Submandibular glands of D. merriami exhibit a sexual dimorphism of the granular tubules. There is little observable difference between the sexes in the species of Perognathus but the ratio of granular tubules to acinar elements, the degree of hypertrophy of the tubules, and the amount of mucosubstance and protein (granules) contained in their cells are different in the four species studied.Since these desert rodents have similar habitats and habits, the differences observed between the two heteromyid subfamilies studied, as well as among the four members of a single subfamily, suggest that these are inherent species variations rather than variations of adaptation to environment.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051310205
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  • 6
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    Nerve growth factor in skeletal tissues of the embryonic chick (1990)
    Springer
    Details
    Publication Date: 1990-01-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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