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  • Column liquid chromatography  (80)
  • wheat  (55)
  • Springer  (135)
  • American Meteorological Society
  • Institute of Physics
  • 1995-1999  (135)
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  • Springer  (135)
  • American Meteorological Society
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  • 1995-1999  (135)
  • 1990-1994
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  • 1
    ISSN: 1573-0832
    Schlagwort(e): Fusarium ; mycotoxins ; occurrence ; trichothecenes ; wheat ; zearalenone ; deoxynivalenol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Wheat for human consumption (140 samples) was collected after harvest from all regions of Bulgaria. The 1995 crop year was characterized by heavy rainfall in the spring and summer months. The internal mycoflora of wheat samples was dominated by Fusarium spp. and Alternaria spp., and storage fungi were rarely present. The samples were analysed for contamination with Fusarium mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), T-2 Toxin (T-2), diacetoxyscirpenol (DAS), and zearalenone (ZEA), using enzyme immunoassay methods. DON and ZEA were the predominant toxins, with a contamination frequency of 67% and 69%, respectively. The average levels of these toxins in positive samples were 180 μg/kg (DON) and 17 μg/kg (ZEA), maximum concentrations were 1800 μg kg−1 and 120 μg kg−1, respectively. Acetyl derivatives of DON, namely 3-AcDON and 15-AcDON, were found in 2.1 % and 0.7% of the samples, at at maximum level of about 100 μg kg−1. Only one sample was positive for T-2 (55 μg/kg), DAS was not detected. This is the first report about the natural occurrence of a range of Fusarium mycotoxins in wheat for human consumption in Bulgaria.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 46 (1996), S. 225-234 
    ISSN: 1573-0867
    Schlagwort(e): long-term experiment ; maize ; wheat ; fertilizers ; farm yard manure ; weedicide application ; yield sustainability ; zinc deficiency ; nutrient uptake ; cropping sequence ; organic carbon build-up
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Long-term field experiments play an important role in understanding the complex interactions of plants, soils, climate and management and their effects on sustainable crop production. A long-term fertilizer experiment with maize-wheat-cowpea (fodder) is in progress since 1971 at Punjab Agricultural University farm Ludhiana, India. The experimental result for the first 21 years showed that application of N alone or in combination with P did not produce as much maize and wheat grains as the application of N, P and K together. Eight years after the start of the experiment, the optimal levels of N, P and K application (100% NPK) were unable to sustain the similar (maize) yield level as before because of Zn deficiency. Whereas in FYM amended plots the Zn deficiency did not appear and the higher crop yields could be sustained. The chemical control of weeds could not sustain the maize productivity at the same level as the manual removal of weeds. It was concluded that the high level of crop production can be sustained with the application of N, P and K under intensive cropping system provided deficiency of any of the micronutrient does not crop up. The deficiency of Zn is most likely to occur in semi-arid light textured alluvial soils under intensive cropping without the addition of farm yard manure/organic manures. In maize based cropping systems, manual control of weeds may be preferred to the chemical one. Addition of FYM in conjunction with 100% NPK is most beneficial both from bio-physical and economic point of view.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Plant molecular biology 30 (1996), S. 1301-1306 
    ISSN: 1573-5028
    Schlagwort(e): abscisic acid ; glycine-rich ; ribonucleoprotein ; RNA-binding protein ; RNP motif ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings. whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Plant molecular biology 31 (1996), S. 575-584 
    ISSN: 1573-5028
    Schlagwort(e): differential display ; heat shock proteins ; non-coding regions ; PCR ; reverse transcription ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific probes from 3′ non-coding regions of different members can assist in the fast retrieval and characterization of individual members of a multigene family. We used the recent technique of differential display for the same purpose. As an example of a multigene family in plants, we selected a heat shock protein gene family, HSP16.9 from wheat, with estimated 12 members. We modified the original differential display technique for selective amplification of the 3′ non-coding regions of different wheat HSP16.9 genes by replacing the random 10-mer in the original method with a conserved HSP16.9 gene family-specific primer. Sixteen cDNA fragments from these experiments were sequenced and they represent 8 different members of a 12 member gene family. Our succes can be attributed to shorter 3′ non-coding regions that are typical of higher-plant genes and use of highly conserved gene family-specific primer in these experiments. This modified differential display technique can be of general application to other plant systems where cloning of the different members of a gene family is desired.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Plant molecular biology 32 (1996), S. 435-445 
    ISSN: 1573-5028
    Schlagwort(e): male sterility ; ovary ; parthenogenetic lines ; pollen allergen ; subtractive hybridization ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To isolate genes specifically expressed at the initiation of plant embryo development we have applied a sensitive subtractive hybridization technique for three isogenic wheat lines of the so-called ‘Salmon system’ with either zygotic or autonomous embryo development. Here we present a gene sequence showing a high homology to grass pollen allergens of type II/III thought to be expressed in pollen tissue only. Surprisingly, the pollen allergen-like sequence, designated Tri a III, is also expressed in gynoecia of the sexual, male fertile wheat line ‘(aestivum)-Salmon’, whereas the two parthenogenetic and male sterile wheat lines ‘(caudata)-Salmon’ and ‘(kotschyi)-Salmon’ completely lack any Tri a III transcript. Our data suggest a positive correlation between the expression of this clone and the manifestation of male fertility. Northern and in situ hybridization analysis revealed that, in addition to its presence in pollen, Tri a III is expressed in the parenchymatous tissue of ‘(aestivum)-Salmon’ ovaries exclusively at the day of anthesis. This precise temporal and spatial expression pattern suggests a more general function of the pollen allergen-like sequence Tri a III not limited to the exhibition of allergens in pollen grains.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    ISSN: 1573-5028
    Schlagwort(e): cDNA ; expression ; FKBP ; PPIase ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell reports 16 (1996), S. 12-17 
    ISSN: 1432-203X
    Schlagwort(e): Triticum aestivum L. ; wheat ; transformation ; biolistics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
    Materialart: Digitale Medien
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  • 8
    ISSN: 1618-2545
    Schlagwort(e): consecutive planting ; Phoma sp. ; plant growth-promotion ; potting medium ; soybean ; wheat ; zoysiagrass rhizosphere fungi
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Several isolates ofPhoma sp., certain nonsporulating fungi, as well asPenicillium andTrichoderma, all isolated from zoysiagrass rhizosphere, promoted growth of wheat and soybean under greenhouse conditions. However, the ability of these rhizosphere fungi to enhance plant growth varied with the crop tested. For example, most of the fungi effectively promoted the growth of wheat, whereas only a few fungi were effective on soybean. In consecutive plantings of wheat and soybean grown in soil previously infested with these zoysiagrass rhizosphere fungi, the growth promotion ability of the fungi was lowered. However, addition of fresh potting medium appeared to restore their growth-promotive effects. It appears that the activation of plant growth-promoting fungi in soil might depend on the availability of organic substrates to colonize, as evidenced by the promotion of plant growth.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1573-6849
    Schlagwort(e): in situ hybridization ; intermediate wheatgrass ; translocation ; virus resistance ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1573-5036
    Schlagwort(e): ammonium ; heterogeneity ; inflow ; localised nutrient ; mineralisation ; 15N ; nitrate ; organic residue ; proliferation ; roots ; soil ; uptake ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract To obtain nutrients mineralised from organic matter in the soil, plants have to respond to its heterogeneous distribution. We measured the timing of nitrogen uptake by wheat from a localised, 15N labelled organic residue in soil, as well as the timing of changes in root length density. We calculated the rates of N uptake per unit root length (inflows) for roots growing through the residue and for the whole root system. A stimulated local inflow appeared to be the main mechanism of exploitation of the residue N during the first five days of exploitation. 8% of the N that the plants would ultimately obtain from the residue was captured in this period. Roots then proliferated in the residue. This, together with a rapidly declining N inflow, contributed to the capture, over the next seven days, of 63% of the N that the plants derived from the residue. After that time, massive root proliferation occurred in the residue, but relatively little further N was captured.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    ISSN: 1573-5036
    Schlagwort(e): evapotranspiration ; grain yield ; irrigation ; plant density ; water use efficiency ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Yield of eight wheat cultivars was evaluated under rainfed and irrigated conditions in a Mediterranean environment. Variation in grain yield resulted from variation in both aboveground biomass production and in harvest index. Under rainfed compared to irrigated conditions, grain yield, biomass and days to heading were decreased, whereas harvest index was increased. Grain yield of the different cultivars under rainfed conditions correlated with that under irrigated conditions in one of the two years. Among cultivars, harvest index under rainfed and irrigated conditions were correlated in both years. Water was used more efficiently for biomass production, and equally efficiently for grain production, under irrigated compared to rainfed conditions. Under rainfed conditions, crop water use efficiency was higher for cultivars developed for rainfed environments than for those developed for high-rainfall or irrigated environments. Cultivars with low-rainfall target environments had the lowest evapotranspiration under rainfed conditions. Under rainfed conditions, differences between the cultivar groups in crop water use efficiency corresponded with trends in water use efficiency of individual plants and with the ratio of photosynthesis to transpiration, measured on plants grown in a growth room. Early in the season, water was used more efficiently for biomass production at high sowing densities than at low sowing densities. Through faster biomass production and ground cover a smaller proportion of the evapotranspired water was lost in soil evaporation and a larger proportion was transpired. However, the net effect was a greater water use in the early phases of growth and consequently a lower water availability later in the season, leading to similar yields regardless of sowing density.
    Materialart: Digitale Medien
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  • 12
    ISSN: 1573-5036
    Schlagwort(e): crown rot ; Fusarium ; fungal disease ; genotypes ; wheat ; zinc ; zinc efficiency
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A crown rot disease in wheat caused by the fungusFusarium graminearum Schw. Group 1 is a widespread problem in chronically Zn-deficient Australian soils. A link between crown rot and Zn deficiency was established by Sparrow and Graham (1988). This paper reports a test of a further hypothesis, that wheat genotypes more efficient at extracting zinc from low-zinc soils are more resistant to infection by this pathogen. Three wheat cultivars (Excalibur, Songlen and Durati) of differential Zn efficiency were tested at three zinc levels (0.05, 0.5 and 2.0 mg Zn kg−1 of soil) and three levels ofF. graminearum S. Group 1 inoculum (0.1 g and 0.3 g kg−1 live chaff-inoculum and control having 0.1 g kg−1 dead chaff inoculum). Six weeks after sowing dry matter production of shoots and roots was decreased byFusarium inoculation at 0.05 mg and 0.5 mg kg−1 applied Zn.Fusarium inoculum at 0.1 g was as effective as 0.3 g kg−1 for infection and decreasing dry matter. The infection at the basal part of culm decreased significantly by increasing the rate of Zn application. Excalibur, a Zn-efficient cultivar (tolerant to Zn deficiency) produced significantly more shoot and root dry matter, and showed less disease infection compared with Zn-inefficient cultivars (Durati and Songlen) at low (0.05 mg Zn kg−1 soil) and medium (0.5 mg Zn kg−1 soil) Zn fertilization rates. Higher rate of Zn fertilization (2.0 mg Zn kg−1 soil) reduced the disease level in Durati to the level of Excalibur but the disease level of Songlen was still high, indicating its high Zn requirement and or sensitivity to crown rot. The data on Zn uptake show that Excalibur, being Zn-efficient, was able to scavenge enough Zn from Zn-deficient soil, we suggest that besides sustaining growth Excalibur was able to build and maintain resistance to the pathogen; inefficient cultivars needed extra Zn fertilization to achieve performance comparable to that of Excalibur. The present study indicates that growing Zn-efficient cultivars of wheat along with judicious use of Zn fertilizer in Zn-deficient areas where crown rot is a problem may sustain wheat production by reducing the severity of the disease as well as by increasing the plant vigour through improved Zn nutrition. ei]Section editor: R Rodriques-Kalana
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 88 (1996), S. 165-174 
    ISSN: 1573-5060
    Schlagwort(e): wheat ; tritordeum ; Hordeum chilense ; Fusarium culmorum ; Septoria nodorum ; scab ; deoxynivalenol ; ergosterol ; resistance ; Triticum spp
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    ISSN: 1573-5060
    Schlagwort(e): Aegilops markgrafii ; aneuploids ; chromosome specific library ; DOP-PCR ; microdissection ; molecular markers ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5α. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.
    Materialart: Digitale Medien
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 1-10 
    ISSN: 1573-5060
    Schlagwort(e): aneuploidy ; chromosome substitutions ; height ; wheat ; yellow rust
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The difficulties in developing inter-varietal chromosome substitution lines in wheat are reviewed. The use of genetical, cytological and molecular markers is suggested as a way of overcoming them. These difficulties and the use of markers, as well as the need to develop duplicate lines to detect background variation, are described using the development and analysis of the Cappelle-Desprez (Bezostaya 1) chromosome substitution set as an example. The effects of substituting Bezostaya 1 chromosomes on final plant height and adult-plant resistance to yellow rust are reported. The large number of aneuploids and substitution lines available in wheat provides a tremendous, international resource, which should be exploited in the future.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 16
    ISSN: 1573-5060
    Schlagwort(e): Bread-making quality ; chromosome effects ; grain hardness ; SDS-sedimentation volume ; substitution lines ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The group 1 and 6 inter-varietal chromosome substitution lines of Cappelle-Desprez (Bezostaya 1) were intercrossed along with the donor and recipient varieties, Cappelle-Desprez and Bezostaya 1, to give 36 genetically different families. The analysis of the means of these families showed that variation in SDS-sedimentation volume fitted a predominantly additive model. There were no significant within or between chromosome interactions among the group 1 and 6 chromosomes. Nor was there any evidence for interactions between these chromosomes and those of the background. Significant dominance/within chromosome interactions amongst the background chromosomes were however detected. Some of the positive effects on SDS-sedimentation were associated with increased grain hardness. Chromosome effects on % grain protein were not correlated with SDS-sedimentation.
    Materialart: Digitale Medien
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 27-31 
    ISSN: 1573-5060
    Schlagwort(e): agronomic characters ; QTL ; RFLP maps ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The advent of molecular marker systems has made it possible to develop comparative genetic maps of the genomes of related species in the Triticeae. These maps are being applied to locate and evaluate allelic and homoeoallelic variation for major genes and quantitative trait loci within wheat, and to establish the pleiotropic effects of genes. Additionally, the known locations of genes in related species can direct searches for homoeologous variation in wheat and thus facilitate the identification of new genes. Examples of such analyses include the validation of the effects of Vrn1 on chromosome 5A on flowering time in different crosses within wheat; the indication of pleiotropic effects for stress responses by the Fr1 locus on chromosome 5A; the detection of homoeologous variation for protein content on the homoeologous Group 5 chromosomes; and the detection of a new photoperiod response gene Ppd-H1 in barley from homoeology with Ppd2 of wheat.
    Materialart: Digitale Medien
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  • 18
    ISSN: 1573-5060
    Schlagwort(e): aneuploids ; automated DNA sequencer ; chromosomal location ; microsatellite marker ; PCR ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is described for bread wheat (Triticum aestivum). The distribution over the different chromosomes and chromosome arms appears to be random. The highest proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified unique fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, amplify fragments on homoeologous chromosomes. In some cases, more than one fragment on a single chromosome or fragments on non-homoeologous chromosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to differentiate between fragments, amplified by a single primer pair, with size differences as small as two base pairs.
    Materialart: Digitale Medien
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 49-57 
    ISSN: 1573-5060
    Schlagwort(e): adaptability ; earliness per se ; photoperiod sensitivity ; vernalization sensitivity ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary In order to obtain high levels of environmental adaptability in wheat varieties it is essential they flower at times appropriate to particular environmental conditions. The influence of three distinct genetic systems that together determine time of flowering is reviewed here. Vernalization genes are seen to be particularly important to winter wheats for their direct or indirect effects on winter hardiness. Vernalization genes play a minor role in determining flowering time in autumn sown winter wheats but insensitivity is essential if spring sown wheats are to flower. Day length sensitive photoperiod genes play a major role in determining flowering time and adaptability of autumn sown wheats. Insensitivity can promote yield advantages of over 35% in Southern European environments. 15% in Central Europe and offers benefits even in the UK. At present only a single allele of Ppd1 appears to have been introduced into commercial European wheat varieties. The merits of alternative Ppd1 alleles or different loci are discussed. The influence of earliness per se genes that determine flowering time independently of environmental stimuli is less well documented than the effect of photoperiod and vernalization genes. It is likely that genes on chromosomes belonging to groups 2, 3, 4, 6 and 7 may act to modify flowering time independently of environmental stimuli probably by determining numbers of vegetative and floral primordia being initiated or the rate of initiation of the primordia. Earliness per se genes appear to be widespread in European wheats and play a significant role in determining the exact time plants flower.
    Materialart: Digitale Medien
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  • 20
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 69-75 
    ISSN: 1573-5060
    Schlagwort(e): dwarfing genes ; gene mapping ; GA insensitivity ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The improvement of lodging resistance by introducing major dwarfing genes, classified either as GA insensitive or GA sensitive, is one of the main strategies chosen by cereal breeders. In the present paper the current knowledge about the genetics, chromosomal localisation and the homoeoallelic relationships of the dwarfing genes in wheat and rye is reviewed. The confusing system of the symbolisation of the GA insensitive dwarfing genes/alleles in wheat is discussed and a nomenclature based on rules for gene symbolisation in wheat is proposed.
    Materialart: Digitale Medien
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  • 21
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 77-80 
    ISSN: 1573-5060
    Schlagwort(e): Chromosomal location ; grain size ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Grain size in wheat is the most stable yield component and has a favorable effect on flour yield. To identify the chromosomes associated with the large grains of line G603-86, (grain weight over 60 mg and grain length of about 9 mm), F3 lines, extracted from F2 populations obtained from F1 monosomics of crosses between G603-86 (P1) and the monosomic set of Favorit (P2) were tested in the field. ANOVA showed significant differences among parents for grain weight and grain length, but not for grain width or the factor expressing the difference in grain form and density. Homoeologous groups had significant effects on grain weight and on all components of grain weight, while genomes were not significantly different for any of these characters. Grain weight was significantly increased by chromosomes 6D and 4A of G603-86. Grain length was significantly increased by chromosomes 4A, 4B, 2B, 3A and 1B, grain width by chromosomes 1A and 1B, and the factor form-density by chromosomes 6D and 6A. The high grain size in G603-86 results from the effects of genes located on many chromosomes which affect grain dimensions, form and density.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 22
    ISSN: 1573-5060
    Schlagwort(e): chromosome substitutions ; hybrid dwarfness ; photoperiodic response ; tissue culture response ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The wheat varieties Chinese Spring, Marquis and Thatcher and five intervarietal ‘Chinese Spring’ substitution lines for chromosomes 2B (2 lines), 2D (2 lines) and 4A*, differing from the recipient variety in alleles for hybrid dwarfing genes and/or the photoperiodic response genes were analysed for tissue culture response (TCR). It could be demonstrated that only chromosome 2B has a major effect on TCR. Allelic variation at the hybrid dwarfing loci seems to have no effect on tissue culture performance, even in the combination D1D2D3 which gives the grass dwarf phenotype. Also the allelic constitution at the Ppd loci, gave no indication for a direct major effect of those alleles, however there seems to exist genetical factors for TCR on the homoeologous group 2 chromosomes which may be closely linked to the Ppd loci.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 23
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 99-102 
    ISSN: 1573-5060
    Schlagwort(e): Triticum miguschovae ; wheat ; leaf rust resistance ; transfer lines ; monosomic analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Triticum miguschovae, a genome addition synthetic, was used as a source for transfer of leaf rust (Puccinia recondita tritici) resistance to common wheat. This synthetic, developed from two wild species Triticum militinae and Aegilops squarrosa, proves a valuable donor of the genes for leaf rust resistance. Leaf rust resistance was transferred from T. miguschovae by both dominant and recessive genes. Stable lines phenotypically similar to their recurrent parents Kavkaz and Bezostaya 1 but differing from them in a high level of leaf rust resistance were obtained. The genes for resistance in 3 selected lines differed from each other and from the known effective genes Lr9, Lr19, and Lr24. The resistance of one of them (line 1229) is controlled by two complementary interacting genes located on chromosome 7B and 1D was revealed by monosomic analysis.
    Materialart: Digitale Medien
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  • 24
    ISSN: 1573-5060
    Schlagwort(e): Aneuploidy ; dwarfing genes ; Triticum aestivum ; preferential transmission ; Aegilops sharonensis ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4S l from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4S l L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.
    Materialart: Digitale Medien
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  • 25
    ISSN: 1573-5060
    Schlagwort(e): C-banding ; FISH ; heterochromatin ; in situ hybridization ; rye ; Secale cereale ; Secale montanum ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The sequential combination of C-banding and in situ hybridization techniques applied in this or in a reverse order, are used to recognize targeted chromosomal regions in cereals. Both methods are described whereby standard chromosome squash preparations are followed by: i) C-banding technique using Leishman stain and a slightly modified in situ hybridization technique using biotin-labeled DNA probes, or ii) fluorescence in situ hybridization technique and C-banding. Both approaches have been successfully used onto mitotic chromosomes of rye and wheat resulting suitable for both their identification and detection of targeted sites.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    ISSN: 1573-5060
    Schlagwort(e): alien introduction ; chromosome pairing ; fluorescent in situ hybridization ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Fluorescent in situ hybridization (FISH) of DNA to plant chromosomes has proved to be a powerful cytogenetic tool. The value of fluorescent in situ hybridization of total genomic DNA (GISH) of related species is demonstrated in the determination of wheat/alien chromosome pairing in hybrids. Its use for assessing the relative merits of the various genes that affect chromosome pairing is also shown. The ability of GISH to identify the presence in wheat of whole alien chromosomes or alien chromosome segments is illustrated. The potential of FISH for detecting repeated DNA sequences, low copy sequences and single copy genes is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 27
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 89 (1996), S. 215-221 
    ISSN: 1573-5060
    Schlagwort(e): agronomic characteristics ; breeding trends ; quality ; year of release ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Wheat breeding efforts for a half century in Japan were investigated by using of 129 varieties registered in MAFF from 1929 to 1984 in the view point of case history for three years. Varieties released in each breeding station are classified apparently by growth habit associated closely with duration of the cold requirement. Heading date is earlier in varieties released in southwest breeding stations (southern varieties) than in varieties released in northern breeding stations (northern varieties). Culm length is higher and the pre-harvest sprouting is more sensitive in northern varieties. In quality characters, northern varieties has the higher milling rate, lower apparent amylose content and larger particle size of flour. In varieties released in the Tohoku district, trend of breeding direction with time is smaller ear numbers and larger 1,000-grain weight and greater resistance to powdery mildew. In varieties released in the Kyushu district, a significant correlation with released year was clearly observed with regard to early maturity, short culm length, less grain crude protein content, less apparent amylose content and higher milling rate on breeding advancement.
    Materialart: Digitale Medien
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  • 28
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia striiformis ; stripe rust ; epidemiology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Ten spring bread wheat cultivars with a susceptible seedling reaction to race 14E14 of yellow rust were tested at three locations to assess the level and stability of quantitative resistance. Quantitative resistance was expressed in terms of disease severity (DS), area under the disease progress curve (AUDPC), apparent infection rate (r), infection type (IT) and infection density. Large genotypic differences were observed for all variables measured. Morocco was the most susceptible cultivar. Based on its high infection type (IT=9) throughout the epidemics, it most likely does not possess any resistance. All other cultivars carry quantitative resistance. The levels ranged from very low (Taichung 23) to very high (Parula). Resistance levels were lower in Quito, Ecuador than at the other locations. Most likely, the lower temperatures in Quito resulted in a reduced expression of quantitative resistance to yellow rust and to obtain the same protection as at the other two locations, more resistance genes are needed. Therefore, to accumulate genes for quantitative resistance, Quito is considered to be the better location. Though significant cultivar-location interactions were detected, they were small compared to the cultivar and location effect. Therefore, they are considered of little importance and it is concluded that quantitative resistance is a stable trait, in the sense that cultivar rankings are hardly affected by environment. The contribution of infection growth to the development of yellow rust was demonstrated. Between 29 and 66% of the increase in disease severity could be contributed to growth of infections. These figures are probably an underestimation of the real contribution as new infections are very small, thus reducing the average size of infections and their contribution to the increase of disease severity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 29
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 91 (1996), S. 31-35 
    ISSN: 1573-5060
    Schlagwort(e): aluminum ; linkage map ; marker ; RFLP mapping ; Triticum aestivum ; Triticum turgidum ; wheat ; durum wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The tolerance of aluminum (Al) of disomic substitution lines having the chromosomes of the D genome of Triticum aestivum L. cv. Chinese Spring individually substituted for their homoeologues in T. turgidum L. cv. Langdon was investigated by the hematoxylin method. The disomic substitution lines involving chromosome 4D were more Al tolerant than Langdon. The tolerance was found to be controlled by a single dominant gene, designated Alt2, that is in the proximal region of the long arm of chromosome 4D. The locus was mapped relative to molecular markers utilizing a population of recombinant chromosomes from homoeologous recombination between Chinese Spring chromosome 4D and T. turgidum chromosome 4B. Comparison of the location of Alt2 in this map with a consensus map of chromosomes 4B and 4D based on homologous recombination indicated that Alt2 is in a vicinity of a 4 cM interval delineated by markers Xpsr914 and Xpsr1051. The Alt2 locus is distal to marker Xpsr39 and proximal to XksuC2. The Altw locus is also proximal to the Knal locus on chromosome 4D that controls K+/Na+ selectivity and salt tolerance. In two lines, Alt 2 and Knal were transferred on a single 4D segment into the long arm of T. turgidum chromosome 4B.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 30
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 91 (1996), S. 127-140 
    ISSN: 1573-5060
    Schlagwort(e): In vitro selection ; salt tolerance ; Triticum aestivum L. ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Embryogenic calli isolated from immature embryos of four wheat cultivars were subjected to three in vitro selection methods for salt tolerance. The effect of NaCl on the selected and unselected cell lines has been investigated. The results indicated that the relative growth rate of callus decreased as the concentration of NaCl increased in both callus lines. The selected callus line gave a higher growth weight in the presence of NaCl in the medium and was highly significant as compared with unselected callus line across medium protocols in all wheat cultivars. The dry weight of both kinds of callus lines of all wheat cultivars increased markedly with increasing NaCl concentration in most cases. The Na+ and Cl- contents of both callus lines were increased with increasing salinity levels while K+ content was decreased. The selected callus line of each cultivar at the same salinity level produced significant amounts of Na+, K+ and Cl- higher than the unselected callus line in most salinity levels. However, the unselected callus lines of the cultivars Giza-157 and Sakha-90 at the same salinity level produced significant amounts of K+ higher than the selected callus line in most salinity levels. The proline content of both kinds of callus lines for all wheat cultivars was increased with increasing salinity level. However, the selected callus line gave a significantly higher proline content than the unselected callus line in all wheat cultivars at the same Salinity level. Results from the in vitro selection for NaCl tolerance showed that the stepwise method of increasing NaCl in the medium was more effective for plant regeneration than other methods.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 31
    ISSN: 1573-5060
    Schlagwort(e): adaptation ; intrinsic earliness ; Triticum aestivum ; vernalization ; wheat ; Syria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary This study sought to identify factors that influence wheat development in the transitional wheat growing zone of northern Syria. Three development factors were studied, intrinsic earliness, and responses to vernalization and to photoperiod. Two sets of wheat were studied, each composed of lines with differing combinations of development factors. Set 1 comprised 20 parental and breeding lines utilized by the CIMMYT/ICARDA facultative and winter wheat breeding program based at Tel Hadya. Set 2 comprised 19 parental and breeding lines utilized by an Australian winter wheat breeding program based at Temora. Field development was recorded in greatest detail at one site. Tel Hadya, using the state of differentiation of the apex of the main tiller of sampled plants. To extend findings, development was also recorded as the time from sowing to ear emergence for later sowings of wheat at Tel Hadya, and in sowings at four other regional sites. The significance of each development factor was tested in multiple regressions that predicted either stage of apical development at Tel Hadya, or time to ear emergence in all trials. It was found that intrinsic earliness was the major factor associated with development, in both sets of wheat. Response to photoperiod had a much smaller and less consistent effect. Response to vernalization had least effect on development, possibly because low temperature in winter delayed development for a longer period than was required to fully vernalize winter wheats. Our results suggested it may not be directly relevant whether spring or winter wheats are grown in the transitional zone of northern Syria. The desired phenotype for the region, of slow development prior to double ridge, then fast development to ear emergence, cannot be simply achieved from combinations of the three development factors. Selection for improved adaptation to the region must continue to rely on direct field observations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 32
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 92 (1996), S. 147-153 
    ISSN: 1573-5060
    Schlagwort(e): breeding methodology ; drought patterns ; input-efficiency ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary About 32% of the 99 million ha wheat grown in developing countries experiences varying levels of drought stress. Three major drought types have been identified: Late drought (LD) is common in the Mediterranean region, early drought (ED) is found in Latin America and wheat is produced on residual soil moisture (RM) in the Indian subcontinent and part of Australia. Until 1983, CIMMYT selected all germplasm under near optimum conditions for its yield potential and tested only advanced lines under drought. In spite of many critics, this approach proved to be successful, since in the mid 80's CIMMYT germplasm was grown on 45% of the wheat area in LC with annual rainfall from 300–500 mm and on 21% in areas with less than 300 mm. Since 1983, CIMMYT's drought breeding methodology is to alternate segregating populations between drought stressed and fully irrigated conditions (FI) and to test advanced lines under a line source irrigation system. To compare the efficiency of these approach, yield of four, mostly leading varieties, from each of the regions with LD, ED, RM, and FI and twelve recent CIMMYT cultivars selected for high yield under FI and RM conditions (ALT) were compared under four different moisture regimes (FI, LD, ED, and RM) in 89–90 and 90–91 in Yaqui Valley, Mexico. Genotypic correlation between yield and days to flowering, days to maturity, height, grains m-2, TKW, test weight and grain fill period were calculated. Mean grain yield of the four best lines in the ALT group was highest under all moisture stress regimes, followed by the FI-group. However, the highest yielding cultivar within each moisture regime was from the FI-group under FI, from the LD-group under LD, and from the ALT-group under ED and RM conditions. Estimates for genetic advance suggest that FI is the best environment for increasing grain yield even in all three drought environments. This indicates that yield potential per se is beneficial also in drought environments. The highest yield in drought environments was realized by the CIM cultivars selected under FI and RM. Simultaneous evaluation of the germplasm under near optimum conditions, to utilize high heritabilities and identify lines with high yield potential, and under stress conditions to preserve alleles for drought tolerance seem at present the best strategy.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 33
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 92 (1996), S. 221-224 
    ISSN: 1573-5060
    Schlagwort(e): hybrid lines ; nitrogen efficiency ; variation ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The new European Common Agricultural Policy and environmental considerations are certainly to change agricultural practices toward low input cultivation systems. Nitrogen is one of the main inputs of winter wheat in northern France and it contributes highly to phreatic water pollution. A research programme has then been set up in order to study whether it is possible to breed for winter wheat cultivars using more efficiently N fertilisers. Less nitrogen would be applied, decreasing pollution risks and operational costs. It has been shown that a large variation exists for N related traits and for the resistance against N deficiency. On the one hand the cv Arche is very resistant to N deficiency, its yield on low N conditions (with no N fertiliser) is on average 89% of its yield on high N conditions (with a high N application). On the other hand, cv Récital is very susceptible to N deficiency as this same percentage is only 61%. A study on 10 hybrids showed that heterosis for grain yield was higher at low N level than at high N level. This was due to a higher number of grains per m2.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 178 (1996), S. 223-230 
    ISSN: 1573-5036
    Schlagwort(e): barley ; cultivar ; acquisition efficiency ; rhizosphere ; root hairs ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract To assess the extent of variation in phosphorus acquisition efficiency of some winter wheat (Triticum aestivum L.), winter and spring barley (Hordeum vulgare L.) genotypes, depletion of inorganic phosphorus (P) extractable with 0.5 M NaHCO3 (NaHCO3-Pi) from the rhizosphere soil was studied. Nutrients supply, rhizosphere soil pH and soil water content was kept equal for all the genotypes with the aim to reduce the confounding variation due to these factors. The experimental set up implied that no difference in the relative growth rates, nitrogen, potassium and calcium content of shoot dry matter occurred among the genotypes. The winter wheat, winter barley and spring barley genotypes differed significantly (p〉0.05) in their efficiency to acquire NaHCO3-Pi from the rhizosphere soil. The efficiency of the winter wheat genotypes to acquire NaHCO3-Pi from rhizosphere soil ranked Kraka 〉 Gawain 〉 Foreman 〉 Sleipner = Obelisk 〉 Kosack 〉 Pepital 〉 Arum. Winter wheat genotypes differed in extent of P depletion profiles in the rhizosphere, indicating variation in root hair length. The winter barley and spring barley genotypes also showed significant differences in their P depletion profiles near roots. The efficiency of the winter barley genotypes to acquire soil P in the rhizosphere ranked Hamu 〉 Frost 〉 Marinka 〉 Astrid 〉 Clarine = Angora. The efficiency of spring barley genotypes to acquire NaHCO3-Pi in the rhizosphere ranked Canut 〉 Etna ≅ Riga 〉 Digger 〉 Peel 〉 Semal 〉 Alexis. The rhizosphere pH remained unchanged, suggesting that additional mechanisms such as root hair formation and root exudates play a significant role in causing variation in P acquisition among the genotypes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 35
    ISSN: 1573-5036
    Schlagwort(e): genotypes ; phytosiderophores ; wheat ; zinc deficiency ; zinc efficiency
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The effect of the zinc (Zn) nutritional status on the rate of phytosiderophore release was studied in nutrient solution over 20 days in four bread wheat (Triticum aestivum cvs. Kiraç-66, Gerek-79, Aroona and Kirkpinar) and four durum wheat (Triticum durum cvs. BDMM-19, Kunduru-1149, Kiziltan-91 and Durati) genotypes differing in Zn efficiency. Visual Zn deficiency symptoms, such as whitish-brown necrosis on leaves and reduction in plant height appeared first and more severe in Zn-inefficient durum wheat genotypes Kiziltan-91, Durati and Kunduru-1149. Compared to the bread wheat genotypes, all durum wheat genotypes were more sensitive to Zn deficiency. BDMM-19 was the least affected durum wheat genotype. Among the bread wheat genotypes, Kirkpinar was the most sensitive genotype. In all genotypes well supplied with Zn, the rate of phytosiderophore release was very low and did not exceed 1 μmol 32 plants-1 3h-1, or 0.5 μmol g-1 root dry wt 3h-1. However, under Zn deficiency, with the onset of visual Zn deficiency symptoms, the release of phytosiderophores was enhanced in bread wheat genotypes up to 7.5 μmol 32 plants-1 3h-1, or 9 μmol g-1 root dry wt 3h-1, particularly in Zn-efficient Kiraç-66, Gerek-79 and Aroona. In contrast to bread wheat genotypes, phytosiderophore release in Zn-deficient durum wheat genotypes remained at a very low rate. Among the durum wheat genotypes BDMM-19 had highest rate of phytosiderophore release. HPLC analysis of root exudates showed that 2′-deoxymugineic acid (DMA) is the dominating phytosiderophore released from roots of Zn-efficient genotypes. In root extracts concentration of DMA was also much higher in Zn-efficient than in inefficient genotypes. The results demonstrate that enhanced synthesis and release of phytosiderophores at deficient Zn supply is involved in Zn efficiency in wheat genotypes. It is suggested that the expression of Zn efficiency mechanism is causally related to phytosiderophore-mediated enhanced mobilization of Zn from sparingly soluble Zn pools and from adsorption sites, both in the rhizosphere and plants.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 36
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; gene action ; heritability ; wheat ; tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Estimates of gene actions were obtained for five in vitro traits of immature wheat (Triticum aestivum L.) embryo cultures from a cross of two wheat cultivars and the resulting reciprocal, F1, F2 and backcross populations. The contribution of additive gene effects to in vitro traits was not as important as the dominance gene effects. Epistatic gene effects were relatively more important than either additive or dominance gene effects. Of the individual types of digenic epistatic effects, the dominance x dominance estimates were relatively larger in magnitude for all in vitro culture traits measured. The maternal effect played a minor role in the inheritance of the in vitro studied traits since the difference among the reciprocal values was not significant. It is shown from the generation mean method that epistasis played a major role in the inheritance of most of the traits under study. The negative values of additive and dominance genetic variance were estimates of zero. Heritability estimates, in broad sense, were relatively high for the in vitro studied traits. In some cases, heritability estimates in broad and narrow senses are almost equal since the estimation of dominance genetic variance led to negative values. According to the results of the gene effects, dominance and epistasis were important for the shoot formation trait. Selection would be effective among the isolated genotypes on individual basis.
    Materialart: Digitale Medien
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  • 37
    ISSN: 1573-5060
    Schlagwort(e): Secale cereale ; Triticum aestivum ; yield components ; 1AL/1RS translocation ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The effect of the 1AL/1RS chromosome translocation on grain yield and other agronomic characteristics of 85 random F2-derived F6 bulks from three 1AL 1RS × 1A bread wheat crosses was determined under optimum and reduced irrigation conditions at CIANO, Yaqui Valley, Sonora, Mexico, during the 1991–1992 and 1992–1993 crop production cycles. Harvest plots of 5.0 m2 were arranged in an alpha lattice design with three replications. The 1AL/1RS translocation increased grain yield, above-ground biomass, spikes/m2, and test weight under both irrigated and dryland conditions. Homozygous chromosome 1A lines, on the other hand, possessed longer spikes with more grains. The 1AL/1RS cultivars had an advantage in 1000-grain weight, which was detected only under optimum irrigation. The translocation lines showed later maturity and longer grainfilling period than the 1A genotypes under one irrigation treatment. A significant relationship between grain yield and test weight was detected only among the 1AL/1RS genotypes, indicating that they possess heavier and plumper grains than the 1A genotypes. These results encourage the continued use of the 1AL/1RS translocation in wheat improvement.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 38
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 90 (1996), S. 315-324 
    ISSN: 1573-5060
    Schlagwort(e): embryogenesis ; wheat ; maize ; Triticum aestivum ; Zea mays ; haploidy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Embryogenesis was analyzed in wheat × maize hybrids using paraffin sectioning. Embryogenesis in wheat × maize hybrids is different from that in self-pollinated wheat plants. Development of the embryo is not accompanied by the formation of an endosperm. The endosperm nuclei remain free in the cytoplasm, fail to advance into the cellular stage, and degenerate at a later time. The antipodal cells quickly degenerate in the fertilized ovaries of wheat × maize hybrids similar to self-pollinated ovaries. The antipodal cells remain normal in unpollinated ovaries. The pre-embryo will abort if it is allowed to develop on the plant, because of a nutritional shortage in the absence of an endosperm. Therefore, embryo rescue is necessary for haploid production from a wheat × maize hybrids. Haploid polyembryos were obtained from spikelet culture of wheat × maize hybrids. The formation of polyembryos is due to the cleavage of the pre-embryo and the effect of 2,4-D. The frequency of haploid embryo production and plant regeneration is affected significantly by maize genotypes, but not by wheat genotypes. The concentration of 2,4-D affects only the size of the embryo.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 39
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 92 (1996), S. 95-103 
    ISSN: 1573-5060
    Schlagwort(e): rusts ; virulence genes ; wheat ; resistance genes ; Czech Republic ; Slovak Republic ; Triticum aestivum ; Thalictrum species
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes of resistance genes in the grown cultivars. Unnecessary genes for virulence had no negative effect on the fitness of the pathogen. All tested samples of aeciospores from barberries attacked rye, not wheat. None of Thalictrum species occurring in the Czech and Slovak Republics was found to host wheat leaf rust. However, the sexual stage of wheat stem rust and wheat leaf rust could be induced on Berberis vulgaris and Thalictrum speciosissimum, respectively. General epidemiological conclusions are drawn from the results and experience of the last 35 years.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 40
    Digitale Medien
    Digitale Medien
    Springer
    Genetic resources and crop evolution 43 (1996), S. 211-220 
    ISSN: 1573-5109
    Schlagwort(e): genetic diversity ; HMW glutenin subunits ; landraces ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Sixty hexaploid wheat landraces collected from five regions of Pakistan were assessed for genetic variability in terms of high molecular weight (HMW) glutenin subunits as revealed by SDS-PAGE. The germplasm appeared to be diverse and unique on the basis of HMW glutenin subunit compositions. Out of 24 alleles detected at all the Glu-1 loci, four belonged to Glu-A1, 12 to Glu-B1 and eight to Glu-D1 locus. The number of novel HMW glutenin subunits detected were 1, 4 and 6 at the three loci (Glu-A1, Glu-B1, Glu-D1), respectively. The frequency distribution patterns of 24 allelic variants detected at the three Glu-1 loci in 1080 samples analysed for 60 accessions were determined both on the basis of individual accessions and on the basis of regions (accessions pooled across the regions). One allele (“null”) at the Glu-A1 locus, three alleles (17+18, 7+8, 14) at the Glu-B1 locus and, two alleles (2+12 and 2**+12′) at the Glu-D1 locus were found most frequently distributed in the 60 populations. Maximum variation was observed in the Baluchistan and Gilgit regions of Pakistan in terms of distribution of novel Glu-1 alleles. A higher gene diversity was observed between the populations as compared to the gene diversity within the populations while, a reverse pattern of gene diversity was observed when populations were pooled across the regions (higher within the regions than between the regions). A data base has been generated in this study which could be expanded and usefully exploited for cultivar development or management of gene bank accessions.
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  • 41
    Digitale Medien
    Digitale Medien
    Springer
    Genetica 97 (1996), S. 243-254 
    ISSN: 1573-6857
    Schlagwort(e): durable resistance ; induced resistance ; leaf rust ; rust resistance ; stem rust ; stripe rust ; wheat ; wheat breeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract It is known that few wheat cultivars maintain their resistance to rust diseases for a long period of time, particularly when crop populations become genetically more uniform. A number of genetically diverse, so far unexploited, sources of rust resistance in the natural as well as mutagenized population of wheat cultivars were identified. Several of these genes were placed in agronomically superior well-adapted backgrounds so that they could be used as pre-breeding stocks for introducing genetic diversity for resistance in a crop population. Some of these stocks when employed as parents in several cross combinations in a breeding programme have generated a number of promising cultivars with diversity for resistance. Many presently grown wheats in India, near-isogenic lines each with Lr14b, Lr14ab, Lr30 and certain international cultivars were identified as possessing diverse sources of adult plant resistance (APR) to leaf rust. Prolonged leaf rust resistance in some of the Indian cultivars was attributed to the likely presence of Lr34 either alone or in combination with other APR components. Tests of allelism carried out in certain cultivars that continue to show adequate levels of field resistance confirm the presence of Lr34, which explains the role that this gene has played in imparting durability for resistance to leaf rust. Also, Lr34 in combination with other APR components increases the levels of resistance, which suggests that combination of certain APR components should be another important strategy for breeding cultivars conferring durable and adequate levels of resistance. A new adult plant leaf rust resistance source that seems to be associated with durability in ‘Arjun’ has been postulated. Likewise, cultivars possessing Sr2 in combination with certain other specific genes have maintained resistance to stem rust. Further, non-specific resistances that were transferred across widely different genotypes into two of the popular Indian wheats provided easily usable materials to the national breeding programmes for imparting durable resistance to stripe rust.
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  • 42
    ISSN: 1573-0867
    Schlagwort(e): China ; Loess Plateau ; maize ; 15N ; Nitrogen ; urea ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Field trials were carried out to study the fate of15N-labelled urea applied to summer maize and winter wheat in loess soils in Shaanxi Province, north-west China. In the maize experiment, nitrogen was applied at rates of 0 or 210 kg N ha−1, either as a surface application, mixed uniformly with the top 0.15 m of soil, or placed in holes 0.1 m deep adjacent to each plant and then covered with soil. In the wheat experiment, nitrogen was applied at rates of 0, 75 or 150 kg N ha−1, either to the surface, or incorporated by mixing with the top 0.15 m, or placed in a band at 0.15 m depth. Measurements were made of crop N uptake, residual fertilizer N and soil mineral N. The total above-ground dry matter yield of maize varied between 7.6 and 11.9 t ha−1. The crop recovery of fertilizer N following point placement was 25% of that applied, which was higher than that from the surface application (18%) or incorporation by mixing (18%). The total grain yield of wheat varied between 4.3 and 4.7 t ha−1. In the surface applications, the recovery of fertilizer-derived nitrogen (25%) was considerably lower than that from the mixing treatments and banded placements (33 and 36%). The fertilizer N application rate had a significant effect on grain and total dry matter yield, as well as on total N uptake and grain N contents. The main mechanism for loss of N appeared to be by ammonia volatilization, rather than leaching. High mineral N concentrations remained in the soil at harvest, following both crops, demonstrating a potential for significant reductions in N application rates without associated loss in yield.
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  • 43
    Digitale Medien
    Digitale Medien
    Springer
    Plant growth regulation 18 (1996), S. 201-206 
    ISSN: 1573-5087
    Schlagwort(e): paclobutrazol ; protection ; stress ; waterlogged ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Wheat (Triticum aestivum L.) seeds were imbibed for 18 h in 40 mM KCl solution containing either distilled water or 100 mg l−1 paclobutrazol (PBZ, a triazole) and then air dried. Seeds were germinated in a greenhouse and one set of seedlings was grown under non-stressed conditions. Another three sets were stressed by waterlogging at three different stages when the PBZ-treated seedlings were 3, 6, or 10 cm above the soil surface. During waterlogging, the soil was saturated by maintaining the water level 3 cm above the soil surface. For waterlogged plants, the first visible symptoms of chlorosis and wilting occurred 2 to 4 days after stress was initiated. There was a concomitant decrease in chlorophyll (a+b) and carotenoid levels. A decrease in fluorescence and increase in leakiness indicated photoinhibitory damage, lower photosynthetic efficiency, and a loss of membrane integrity. These symptoms of stress induced by waterlogging at different stages were reduced by PBZ.
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  • 44
    ISSN: 1573-5060
    Schlagwort(e): gramineae ; polyembryony ; somatic embryogenesis ; Triticum aestivum ; wheat ; genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to developing spikes, it was found that three cultivars showed a different ability to produce polyembryos (Thatcher 20.19%, Chris 7.06%, Dollar 0%). This behaviour was related to their capacity to form somatic embryos. Diploid immature embryos cultured in vitro after 2,4-D treatment, gave a higher frequency of embryogenic callus in Thatcher and Chris than in Dollar. As the common factor in both experiments was the 2,4-D treatment we propose that the three cultivars showed a differential sensitivity to 2,4-D.
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  • 45
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 92 (1996), S. 175-183 
    ISSN: 1573-5060
    Schlagwort(e): adaptation ; durable resistance ; N-use efficiency ; stability ; wheat ; yield potential ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The wheat area in developing countries, including China, is around 100 million ha. To address the needs of these very diverse wheat growing areas, CIMMYT has defined 12 wheat mega-environments (ME). A ME is defined as broad, not necessarily continuous often transcontinental area with similar biotic and abiotic stresses, cropping systems and consumer preferences. The factors describing each ME are presented. CIMMYT's breeding methodology is centered around the development of widely adapted germplasm with high and stable yield across a wide range of environments. Segregating populations are alternating screened in two diverse environments in Mexico. One key requirement is that all germplasm is tested under near optimum conditions for its yield potential. The second one is multi-locational testing of advanced lines at sites that represent a given ME (key locations) and careful screening of germplasm for tolerance to abiotic and biotic stresses specific to that environment. This methodology has permitted the pyramiding of a large number of multiple resistance genes for use against a wide spectrum of diseases and tolerance to abiotic stresses within each ME. In addition, the widespread testing of lines allows the identification of traits which are beneficial in several environments. Data from international nurseries are used to further delineate environments within an ME. This approach has proven to be successful since around 70% of the spring wheat area in developing countries (excluding China) is planted to varieties derived directly or indirectly from CIMMYT germplasm. The performance of the bread wheat cultivar Pastor in international trials is given as an example for a wide adaptation.
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  • 46
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 88 (1996), S. 69-76 
    ISSN: 1573-5060
    Schlagwort(e): dwarfing genes ; late maturity α-amylase ; gibberellic acid insensitivity ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Two wheat cultivars, Spica and Lerma 52, which consistently produce high levels of α-amylase during the later stages of grain development (late maturity α-amylase), were crossed with a set of four near-isogenic lines carrying the tall (rht) allele or one of the dwarfing genes Rht1, Rht2 or Rht3 (GA-insensitive alleles). The F1 and F2 populations were developed and analysed for grain α-amylase and plant height. The Rht3 gene exhibited the strongest influence on plant height and strongly inhibited new α-amylase synthesis during the later part of grain ripening. By comparison, Rht1 and Rht2 had a less pronounced effect but still significantly reduced the expression of late maturity α-amylase. These observations suggest that gibberellic acid is involved either directly or indirectly in this phenomenon. The implications of the effect of dwarfing genes on expression of late maturity α-amylase are discussed in relation to cultivar improvement and to the identification and control of high α-amylase germplasm.
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  • 47
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 88 (1996), S. 61-67 
    ISSN: 1573-5060
    Schlagwort(e): inheritance ; late maturity α-amylase ; wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Two wheat cultivars that consistently show high levels of grain α-amylase at harvest ripeness, in the absence of preharvest sprouting, were crossed with a control, low α-amylase cultivar, and F1, F2 and BC1 populations were developed. Grain of these populations was analysed for α-amylase activity at harvest ripeness. Distribution and segregation patterns were consistent with control at a single locus with high α-amylase the recessive allele. This mode of inheritance would make it extremely difficult to differentiate homozygous low α-amylase lines from heterozygotes (low α-amylase phenotype but carriers of high α-amylase) and has important implications for wheat breeders. High α-amylase, termed late maturity α-amylase, was not linked with the awned inhibitor gene, B2, located on the long arm of chromsome 6B.
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  • 48
    ISSN: 1573-5060
    Schlagwort(e): crossability ; kr1 gene ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The recessive of crossability allele kr1 was transferred from the spring wheat variety Chinese Spring (CS) into the winter wheat variety Martonvásári 9 (Mv9) by backcrossing the Mv9 × CS hybrids with Mv9. The Mv9 variety possesses dominant Kr1 alleles and is heterogeneous at the kr2 locus, so that some individual plants carry recessive kr2 alleles. The selection of plants possessing the recessive kr alleles from the (Mv9 × CS)Mv9 BC1 generation was carried out according to the seed set achieved when pollinated with rye (Secale cereale L. cv. Mercator). The partial dominance of the Kr alleles made it possible to differentiate between plants heterozygous at the Kr1 locus and Kr1Kr1 homozygous dominant plants. Two selfed consecutive progenies were tested by pollination with rye to select the homozygous recessive kr1kr1kr2kr2 plants and to check the result of the selection after each backcross. As a result of three backcrosses with Mv9 and two selfings after each backcross the selected progenies had 61.6% seed set with rye tested on sixty individual plants. These data confirm that after the third backcross the selected Mv9 kr1 line carries necessive crossability alleles Kr1 and Kr2, but the genotype is 93.75% Mv9.
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  • 49
    ISSN: 1573-5060
    Schlagwort(e): electrophoresis ; gluten strength ; prolamins ; Triticum aestivum ; variation ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Allelic variation of prolamin loci was examined in the F2 from crosses between the hexaploid wheat varieties: ‘Cajeme 71’, ‘Yécora 70’, ‘Ablaca’, ‘Anza’, ‘Pané 247’ and ‘Axona’. Different allelic blocks for gliadins and LMW glutenin subunits were determined in Gli-1, Gli-2 and Glu-3 loci. A percentage of recombination of 1.5 ± 0.3 was determined between Gli-A1 and Glu-3 in the F2 progeny of ‘Yécora 70’ x ‘Axona’. A significant positive association was found between gluten strength, measured by SDS-sedimentation volume, and the prolamins coded by ‘Anza’ Gli-D1/Glu-D3 loci and ‘Yécora 70’ Gli-A1/Glu-A3 loci. Interactions between non homeologous loci Glu-1 and Gli-1/Glu-3 were also found.
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  • 50
    ISSN: 1573-5060
    Schlagwort(e): chromosomal arm location ; chromosome location ; ditelosomic analysis ; monosomic analysis ; Septoria glume blotch ; Stagonospora nodorum ; Triticum aestivum ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Septoria glume blotch, caused by Stagonospora nodorum, is an important disease of wheat (Triticum aestivum). Separate genetic mechanisms were found to control flag leaf and spike resistance. Genes for resistance to S. nodorum were located on different chromosomes in the few wheat cultivars studied. These studies only partially agree on the chromosome locations of gene in wheat for resistance to S. nodorum, and chromosomal arm locations of such genes are not known. The objectives of this study were to determine the chromosome and chromosomal arm locations of genes that significantly influence resistance to S. nodorum in wheat cultivar Cotipora. Monosomic analysis showed that flag leaf resistance was controlled by genes on chromosomes 3A, 4A, and 3B whereas the spike resistance was controlled by genes on chromosomes 3A, 4A, 7A, and 3B (P=0.01). Additionally, genes on chromosomes 6B and 5A influenced the susceptibility of the flag leaf and spike reactions, respectively (P=0.01). Telocentric analysis showed that genes on both arms of chromosome 3A, and the long arms of chromosomes 4A and 3B were involved in the flag leaf resistance whereas genes on both arms of chromosome 4A, the short arm of chromosome 3A, and the long arm of chromosome 3B conferred spike resistance.
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  • 51
    ISSN: 1573-5060
    Schlagwort(e): Fusarium resistance ; Fusarium spp. ; wheat ; Triticum aestivum ; culture filtrate ; in vitro selection ; somaclonal variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Calli of resistant, intermediary and susceptible wheat (Triticum aestivum L.) varieties were selected using culture filtrates of Fusarium graminearum and F. culmorum and the regenerants were evaluated for resistance up to R3. Czapek-Dox broth medium was inoculated with mycelia of Fusarium isolates and incubated for 2–6 weeks. Filtrates were added to MS callus growing medium, then 5 weeks-old calli were transferred onto this medium (MST) for 4–5 weeks. MST containing 30% filtrate was found to be suitable for selection. Resistant calli were transferred again to fresh MST for further two selection cycles. The surviving calli produced less fertile regenerated lines (R0) than the non-selected ones. Among 18 R1 lines tested for Fusarium-resistance in the seedling stage by artificial inoculation in the greenhouse, two (11.1%) were significantly more resistant, one (5.6%) was more susceptible than the original cultivar and the rest (83.3%) behaved similarly to the donor plants. Among unselected R3 lines of three varieties, practically the same number of resistant plants were found as among the related selected ones. When the R3 selfed generations obtained through double-layer and culture filtrate selection techniques were tested for Fusarium-resistance, 35.7% of the lines were found to be more resistant than the original cultivars, none was more susceptible and 64.3% had a reaction similar to that of the source materials. Thus, inheritance of the disease reaction was not stable in all cases. Success of in vitro selection for Fusarium-resistance depended also on the genotype, and toxin analysis showed that although being effective, the selective media contained deoxynivalenol only exceptionally. In selecting wheat for Fusarium-resistance in vitro, the culture filtrate technique proved better than the double-layer procedure.
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  • 52
    Digitale Medien
    Digitale Medien
    Springer
    Photosynthesis research 47 (1996), S. 281-290 
    ISSN: 1573-5079
    Schlagwort(e): elevated CO2 ; FACE ; photosynthetic acclimation ; wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A simple system for free air carbon dioxide enrichment (FACE) was recently developed and it is here briefly described. Such a MiniFACE system allowed the elevation of CO2 concentration of small field plots avoiding the occurrence of large spatial and temporal fluctuations. A CO2 enrichment field experiment was conducted in Italy in the season 1993–1994 with wheat (cv. Super-dwarf Mercia). A randomized experimental design was used with the treatment combination CO2 × soil N, replicated twice. Gas exchange measurements showed that photosynthetic capacity was significantly decreased in plants exposed to elevated CO2 and grown under nitrogen deficiency. Photosynthetic acclimation was, in this case, due to the occurrence of reduced rates of rubP saturated and rubP regeneration limited photosynthesis. Gas exchange measurements did not instead reveal any significant effect of elevated CO2 on the photosynthetic capacity of leaves of plants well fertilized with nitrogen, in spite of a transitory negative effect on rubP regeneration limited photosynthesis that was detected to occur in the central part of a day with high irradiance. It is concluded that the levels of nitrogen fertilization will play a substantial role in modulating CO2 fertilization effects on growth and yields of wheat crops under the scenario of future climate change.
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  • 53
    Digitale Medien
    Digitale Medien
    Springer
    Water, air & soil pollution 92 (1996), S. 63-73 
    ISSN: 1573-2932
    Schlagwort(e): China ; agriculture ; maize ; rice ; wheat ; GFDL ; MPI ; UKMO
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik
    Notizen: Abstract This paper discusses the vulnerability and adaptation of the agricultural sector of China to global warming. Based on a summarization of Chinese agricultural and general circulation model trends, adverse impacts on China's agriculture caused by a warming and drying climate were identified. Because of limited irrigation potential, the sustainable development of Chinese agriculture will be difficult. Six sensitive agricultural areas located on the edges of different agroecological zones, and seven provinces with high vulnerability to the impacts on agriculture, were identified. On the basis of an estimation ofthe potential supply of agricultural products and demand for food, the annual incremental costs for adaptation to climate change would be US$0.8–3.48 billion; without adaptation, the annual agricultural loss due to global warming would be US$1.37–79.98 billion from 2000 to 2050. Adaptive measures discussed include intensive management and the possibility of a tripartite structure of planting that would entail coordinated development of gain crops, feed crops, and cash crops.
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  • 54
    Digitale Medien
    Digitale Medien
    Springer
    Water, air & soil pollution 92 (1996), S. 53-62 
    ISSN: 1573-2932
    Schlagwort(e): Bangladesh ; agriculture ; rice ; wheat ; CCCM ; CERES-Rice ; CERES-Wheat ; GFDL
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik
    Notizen: Abstract A simulation study was conducted to assess the vulnerability of foodgrain production in Bangladesh to potential climate change. Simulation runs were made for high yield varieties office for Aus (March-August), Aman (July-November), and Boro (February-July), the traditional growing seasons, using the CERES-Rice model. Simulation runs were made for wheat, which is grown from November through March, using the CERES-Wheat model. Three scenarios (baseline, Canadian Climate Centre Model, and Geophysical Fluid Dynamics Laboratory) and sensitivity analyses for temperature increases of 2 and 4°C at three levels of CO2 (330, 580, and 660 ppm) were used. In the simulation, increased CO2 levels increased rice yields over baseline, and considerable spatial and temporal variations were noted. Higher temperatures reduced the yields in almost all study locations and in all seasons, and it was particularly pronounced with a 4°C increase. The detrimental effect of temperature rise was observed even with increased CO2 levels. Wheat yields increased with increased CO2 level in all three locations. The adverse effects of increased temperature were more pronounced for wheat than for rice at all levels of increased CO2. In the scenarios of the Canadian Climate Centre Model and Geophysical Fluid Dynamics Laboratory, both rice and wheat yields were decreased (35% and 31 %, respectively) over baseline in all seasons, especially in the Aus season, and in all locations. The adverse effects of the climate under the Geophysical Fluid Dynamics Laboratory scenario were more pronounced for wheat that for rice. The greatest reductions in aggregated production for both crops were noted at 330 PPM CO2 with a 4°C temperature rise. The greatest increases in aggregated production for rice and wheat were observed at a 660 ppm CO2 with no temperature increase.
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  • 55
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Heterocyclic aromatic amines (HAA) ; Electrospray MS-MS detection ; Selected reaction monitoring (SRM)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Sensitive and selective detection of the sixteen most abundant heterocyclic aromatic amines (HAA) has been achieved by application of high performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) in combination with selected reaction monitoring (SRM). Detection limits between 0.1 and 50 ng mL−1 were established by use of HAA model solutions.
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  • 56
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Column switching ; Step-gradient elution ; Optimization ; Trace analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A computer spreadsheet application has been developed for the optimization of step-gradient elution conditions as applied in coupled-column RPLC for online clean-up and separation in the analysis of pesticide residues. The procedure is based on the experimentally determined retention behaviour of the analytes as a function of mobile phase composition. Retention and peak volumes of the analytes eluting under isocratic and one- and/or two-step gradient conditions can be calculated. For a given set of analytes, the spreadsheet systematically calculates the resolution of the least efficiently separated pair of peaks in the space defined by the eluotropic strengths of the mobile phases and the time at which the step-gradient takes place. The performance of the proposed procedure was tested and evaluated for the analysis of nine polar pesticides for which in a previous study adequate conditions had been found by computer-aided chromatogram simulation. the optimum conditions predicted by the procedure were in good agreement with those established experimentally and by simulation. The simultaneous production of data on retention, peak volume and resolution for a group of analytes under selected one- and/or two step-gradient elution conditions makes the calculation procedure highly productive and flexible in searching for and/or adjusting appropriate separation conditions.
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  • 57
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Hydrous zirconium oxide ; Porous polymer resin ; Disubstituted phenols ; ortho-Benzoic acid derivatives
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A porous polymer resin loaded with hydrous zirconium oxide (Zr-gel) was evaluated as a stationary phase for the liquid chromatographic separation of disubstituted aromatic compounds. The prepared Zr-gel was used to separate disubstituted phenols andortho-substituted benzoic acid derivatives in reversed-phase mode. The retention time of catechol was greater than those of other disubstituted phenols; this implies that the Zr-gel has specificity for the phenolic hydroxyl group. The retention behavior ofortho-substituted benzoic acid derivatives on the Zr-gel was also evaluated in reversedphase mode using buffer. Phthalic acid was specifically retained in the vicinity of pH 6.0, which has been regarded as the equal adsorbic point. Similar behavior was also observed for salicylic acid, although the retention time of salicylic acid was less than that of phthalic acid. It seems that the specific retention behavior of hydrous zirconium oxide is a result of complexation with ligands such as hydroxyl and carboxyl groups. The results of this study have revealed that the retention mechanism of hydrous zirconium oxide is a combination of ion exchange and interaction based on complexation with ligands.
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  • 58
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Tryptophan ; Chiral separations ; Diode-laser polarimetric detection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The combined utilization of photometric, fluorimetric and polarimetric detection liquid chromatography detectors in series for the identification and quantification of D-tryptophan and L-tryptophan was evaluated. Detection limits of about 1 μg were established with the range of linearity extending to about 100 μg. The relative standard deviation of the D-form and L-form tryptophan were 7.32 and 4.22%, respectively. The amount unknown of tryptophan enantiomers in the different mixtures was determined with an accuracy of 1.58% at the 40 μg injection level.
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  • 59
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Un-sulphated and sulphated surfactants ; Oligoethyleneglycol nonylphenyl ethers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Retention behaviour of un-sulphated and sulphated, oligoethyleneglycol nonylphenyl ether, anionic surfactants was investigated in normal-phase LC systems with chemically bonded amino stationary phase in organic mobile phases containing water and cetyl trimethylammonium bromide (CTAB). Addition of water to the mobile phase improves efficiency of separation and linearity of the dependences of logk′ versus number of oxyethylene units in the sulphated oligomers, so that it is possible to describe simultaneous effects of this number and of the concentration of CTAB on retention by a simple equation. In this system, it is possible to separate lower sulphated and un-sulphated oligomers in a single run in 10–15 min under isocratic conditions.
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  • 60
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Electrothermal atomic absorption spectrometry ; Vitamin B12 analogues ; Cobalamins and cobalt ; Meat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The coupling of liquid chromatography and electrothermal atomic absorption spectrometry (LC-ETAAS) lowers the detection limit for identification of vitamin B12 analogues. Cobalamins and aqueous cobalt (II) were separated by reversed-phase liquid chromatography using a linear gradient: 26∶74 (v/v) methanol:0.05 M phosphate buffer (pH 4.2) to 50∶50 mixture over 8 min. The vitamins were quantitatively determined in the column effluent by measuring total cobalt by ETAAS. The analysis of meat and liver extracts by LC-ETAAS showed that the matrix did not interfere with the determination of cobalt. Hence, recoveries of cobalt in spiked meat and liver samples were satisfactory.
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  • 61
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 42 (1996), S. 405-408 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Meropenem in rat plasma ; Column-switching
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The purpose of this study was to develop a columnswitching HPLC method for the determination of meropenem in plasma. This method showed excellent precision and accuracy with good sensitivity and speed. The total analysis time per sample was less than 20 min and the mean coefficients of variation for intra- and inter-assay were less than 4.0%. The method has been successfully applied to plasma samples from rats receiving an intraperitoneal injection of meropenem.
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  • 62
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Thiol drugs ; Pre-chromatographic derivatization ; 1,1′-[ethenylidenebis(sulfonyl)]bis-benzene reagent
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The use of 1,1′-[ethenylidenebis(sulfonyl)]bis-benzene as a pre-chromatographic reagent for LC analysis of thiol drugs is proposed. The reagent reacts rapidly (2 min) under mild conditions (pH 7.5, ambient) with thiol drugs and the resulting adducts can be chromatographed under reversed-phase conditions (C-18 column). Excess reagent can be removed from the reaction mixture by simple liquid-liquid extraction with chloroform. The HPLC method using UV detection was successfully applied to the analysis of reduced glutathione (GSH), N-acetylcysteine, N-2-mercaptopropionylglycine and captopril in their commercial formulations.
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  • 63
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; 2-Arylpropionic acids ; Barbituric acids ; Benzodiazepines ; Cellulose tris(4-methylbenzoate) chiral stationary phase ; Reversed-phase mode ; Chiral recognition mechanisms
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A newly developed reversed-phase chiral stationary phase, cellulose tris(4-methylbenzoate), known as Chiralcel OJ-R, has been successfully applied to the direct resolution of the enantiomers of several nonsteroidal anti-inflammatory drugs belonging to the profen group. Among the five barbituric acids tested, the chiral resolution of mephobarbital is especially high. The Chiralcel OJ-R column also proved quite efficient for the enantiomeric resolution of a few representatives of the benzodiazepine group. Variations of mobile phase conditions based on acetonitrile and/or methanol were tested. The results obtained are briefly compared with separations performed using a Chiralcel OJ column under normalphase conditions.
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  • 64
    ISSN: 1612-1112
    Schlagwort(e): Liquid-liquid and liquid-solid extractions ; Column liquid chromatography ; Mass spectrometry detection ; Flavonoids inPhillyrea angustifolia leaves
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Liquid-liquid (LLE) and liquid-solid extraction (LSE) procedures were developed to identify polyphenols in phillyrea (Phillyrea angustifolia L.) leaves. The liquidsolid extraction, carried out by using two serial Bond-Elut® CH and Bond-Elut® SAX cartridges allowed the collection of both polyphenols and soluble carbohydrates during a single extraction of leaf. This method seems to be suitable for both phytochemical and physiological study of the species, allowing the identification of two classes of organic compounds which have a central role in the metabolism of phillyrea plants. A method of isolation and concentration of polyphenols for further molecular characterization, using centrifuge chromatography, was also developed. Molecular characterization, carried out by UV-Vis spectrophotometry and mass spectrometry, demonstrated the presence of compounds with interesting biological activity, i.e. flavonoids and oleuropein derivatives. The soluble carbohydrate composition of phillyrea leaves did not substantially differ from that ofOlea europaea L. leaves, except for a higher mannitol/glucose ratio. This carbohydrate distribution may be linked to the evolution pattern of this species which usually grows in severely stressed environments.
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  • 65
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; On-line liquid chromatography-mass spectrometry ; Neutral and acidic cannabinoids ; Hashish samples
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary This paper reports the use of liquid chromatography for the separation and determination of the major cannabinoids extracted from hashish samples. The direct coupling to the mass spectrometer enables the selective identification both of neutral and acidic cannabinoids. The developed method does not require any preliminary derivatization and should, therefore, be of interest in forensic analysis for simple and unequivocal determination of hashish constituents.
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  • 66
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Non-linear adsorption isotherm ; Simulation of chromatographic peak profiles ; Separation of optical isomers ; Non-racemic mixtures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary This article discusses the effect of solute association on the apparent adsorption isotherm and on the chromatographic peak profile. The modelling performed has shown that dimerization of a solute in a chromatographic system gives rise to an apparent adsorption isotherm of the non-linear type. This results first of all in peak broadening. Tailing or a fronting can be observed depending on the relative retention of the monomer and the dimer. Secondly, the retention of the solute depends on the amount of sample because of the non-linear character of the adsorption isotherm. As a result, an excess of one of two analytes with absolutely identical adsorption properties i.e. optical isomers, can be separated in such chromatographic systems by considering that the sample contains non-equal amounts of two analytes. The phenomenon is illustrated by simulated chromatograms of the separation of non-racemic mixtures of enantiomers.
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  • 67
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Hydrous zirconium oxide ; Porous polymer resin ; Silica gel ; ortho-Benzoic acid derivatives
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Silica gel loaded with hydrous zirconium oxide (Zr-gel-S) has been prepared and the influence of the base material on the retention behavior ofo-phthalic acid on the hydrous zirconium oxide was evaluated. The retention behavior of the acid on the Zr-gel-S was compared with that on Zr-gel-5, the polymer-based Zr-gel. Comparable retention behavior,i.e. maximum retention in the vicinity of pH 6, which has been regarded as the equal adsorbic point, was observed for both types of Zr-gel, although there was a large difference in the retention times. The difference in retention on the two types of Zr-gel could be ascribed to nature of the base material. The results in this experiments showed, however, that the specific behavior at pH 6 was an essential characteristic of hydrous zirconium oxide and was not related to the influence of the base materials.
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  • 68
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Sterols, tocopherols, retinols and carotenes ; Dairy products
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary We have previously described a normal-phase HPLC method for the simultaneous determination of α, β, γ and δ tocopherol, thecis andtrans isomers of retinol, and total carotenes in different Italian cheeses and milk. In this paper we describe a reversed-phase HPLC method using solely methanol as the mobile phase for the determination of α-tocopherol, β-+γ-tocopherols, total retinols, cholesterol, β-sitosterol, campesterol and stigmasterol, and α- and β-carotene. The method developed has been applied to some new dairy products such as natural Quark, with olive, basil or banana and to processed cheese with tomato and the results have been compared with the corresponding results obtained by the normal-phase method. The conclusion is that more complete evaluation of the tocopherol, retinol, carotene and sterol content of dairy products, especially when vegetables and fruits are present, is necessary and can be obtained using both the normal- and reversed-phase HPLC methodologies.
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  • 69
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Diode-array detection ; Particle beam mass spectrometric detection ; Pesticides in water ; On-line solid-phase extraction (SPE)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A group of pesticides has been determined by liquid chromatography with diode-array detection (DAD). The coupling of a particle-beam (PB) mass spectrometer to the outlet of the DAD enabled the identities of the pesticides to be confirmed from mass spectra obtained by electron impact ionization. On-line trace enrichment of 200 mL of sample with a PLRP-S precolumn enabled most pesticides to be determined in real samples at levels between 0.2 and 5 μg L−1 and with limits of detection (LOD) between 0.05 and 0.5 μg L−1. In the analysis of real samples, one of the pesticides studied was determined by DAD and its identity was confirmed by comparison of PB mass spectra. The use of PB-MS also enabled the detection of non-target compounds.
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  • 70
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Capillary electrophoresis ; Phenolic compounds ; Water samples
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A comparative study of high-performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE) for determination of eleven EPA priority phenols in water samples is discussed. The repeatability, linearity and detection limits of both methods are compared. A solid-phase extraction method using a highly cross-linked styrene-divinylbenzene copolymer sorbent is used for the off-line preconcentration process in order to determine these compounds in water at low levels. Both methods are used to determine phenolic compounds in Ebro river and industrial waste water. Identification was achieved by comparison of the UV spectra corresponding to the different peaks, acquired with a diode-array detector, with those in a spectral library.
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  • 71
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Polycyclic aromatic hydrocarbons ; Waste waters ; Sewage sludge
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A modified solid-phase extraction technique using sonication of the adsorbent material instead of the elution normally applied has been compared with two conventional liquid-liquid extraction procedures for the determination of the 16 EPA PAHs in municipal waste waters by means of HPLC coupled with fluorescence detection. Liquid-liquid extraction with cyclohexane proved to be the most efficient and simplest procedure. Clean-up of the waste-water extracts was not considered necessary, because of the high chromatographic resolution of the column and the selectivity of the fluorescence detector. Different organic solvents were also compared for ultrasonic extraction of PAHs from sewage sludge. The best results were obtained by use of acetonitrile. Clean-up of sewage-sludge extracts was not found necessary for accurate quantification of the major PAH components with fluorescence detection. The precision of the whole analytical procedure from extraction to the final determination of PAHs was satisfactory for both waste-water and sewage-sludge samples.
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  • 72
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 43 (1996), S. 117-123 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Ion-exchange chromatography ; Ion-exchange mechanism ; Optimization of alkali and alkaline earth separation ; Crown ether complexing reagents
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The elution behavior of alkali and earth alkaline cations has been studied on a newly synthesized weak cationexchanger (WCX). Applying this stationary phase isocratic separation of these cations is possible. The dependence of retention on competing ion concentration is described via a simple model. The influence of crown ethers as well as organic eluent additives on selectivity and peak efficiency is demonstrated. With optimized separation such cations can be determined with detection limits of about 0.5 ppm using a conductivity detector without ion suppression.
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  • 73
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 43 (1996), S. 143-148 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Micellar liquid chromatography ; Ion-exchange-induced stationary phase ; Dansyl amino acids (Dns-AAs) ; Fluorescence enhancement
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The retention behavior of dansyl amino acids in micellar liquid chromatography has been examined by using ionexchange-induced stationary phases. Several parameters affected the retention of the analytes, including the type and concentration of micellar agent and modifier ion and the concentration of acetonitrile in the mobile phase. The order of elution of dansyl amino acids obtained with the micellar mobile phase was very different from that observed in conventional reversed-phase liquid chromatography. Fluorescence intensities of some dansyl amino acids were enhanced by the micellar mobile phase.
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  • 74
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 43 (1996), S. 551-556 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Chemically bonded silica gel ; Mixed solvents ; Steroids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The chromatographic behaviour of 30 estradiol and estrone derivatives has been studied on diol, amino- and cyanopropyl silica gel stationary phases with non-aqueous mobile phases. The slopes of linear relationships between the logarithm of the retention factor and logarithm of the volume fractions of the polar components of the binary eluents have been calculated and are discussed in relation to the characteristics of the solute, eluent and stationary phase. An equation for testing the separation efficiency of stationary phases has been derived.
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  • 75
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Substituted benzenes ; Solubility parameter ; Substituent constant
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A quantitative structure-retention model derived from the concept of hydrophobic substituent constant (π) and the total solubility parameter (δT) has been established. It has been used to predict successfully the retention behavior of a number of solutes on various columns in several different eluents. The experimental results indicate that the model can be employed for the prediction of retention of substituted benzenes in a large volume fraction range of mobile phase. In addition, the physical meaning of the sign and the influence of the coefficients of the model on the retention process had been discussed together with the dependence of these coefficients on the composition of mobile phase.
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  • 76
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Fullerences ; Alkyl bonded silica phases ; Retention vs. column temperature ; CP-MAS NMR
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The temperature-dependency of the separation of fullerenes in liquid chromatography (LC) has been examined using various alkyl bonded stationary phases. It has been found that a maximum retention temperature exists with long alkyl bonded stationary phases, whereas there is no similar effect with the newly synthesized alkyl bonded phases which have two phenyl groups at the base of the bonded phase. The interpretation of the retention behavior of fullerenes in the low temperature region on alkyl bonded stationary phases is discussed using information obtained by CP-MAS solid-state NMR spectroscopy and LC.
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  • 77
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 42 (1996), S. 683-689 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Chiral stationary phase ; Enantionary phase ; Enantiomer separation ; Amino acids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Two chiral stationary phases (CSP-1 and CSP-2)) were synthesized by covalent bonding of aminopropylsilica (APS) to (a) 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's reagent), followed by end-capping of underivatized amino groups of APS with trifluoroacetic anhydride (CSP-1), and (b) by reaction of APS with 1-fluoro-2,4-dinitrophenyl-5-L-phenylalaninetert. butyl ester, followed by cleavage of the tert. butyl ester by trifluoroacetic acid and end-capping of underivatized amino groups of APS withn-butyryl chloride (CSP-2). Both CSPs were found to be suitable for the chiral resolution of 2,4-dinitrophenyl and 3,5-dinitrobenzoyl-DL-amino acid esters by liquid chromatography using mixtures ofn-hexane andiso-propanol as eluents. Racemic 2,2,2-trifluoro-1-(9-anthryl)ethanol was also resolved on CSP-2.
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  • 78
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Calcium and lead phosphate hydroxyapatite ; Globular proteins ; Colecalciferol (Vitamin D3) and related compounds ; Organophosphorus insecticides
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A qualitative study using two new stationary phases (spherical aggregates of calcium phosphate hydroxy-apatite and lead phosphate hydroxyapatite) as adsorbents in high performance liquid chromatography has achieved the separation of: a standard mixture of globular proteins; colecalciferol and related compounds (produced by irradiation and heating of provitamin D3); and a mixture of organophosphorus insecticides used in agriculture.
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  • 79
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 42 (1996), S. 199-205 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Negative ion CI mass spectrometry ; Oxidizedd-limonene ; Contact allergenic compounds
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A detailed chemical analysis and quantification of oxygen-containing monoterpenes in auto-oxidizedd-limonene was performed. Some of these, e.g. the cis- and trans-isomers of limonene-2-hydroperoxide, have previously been shown to exhibit strong contact allergenic properties. GC-MS with chemical ionization in negative ion mode was shown to be a successful method for the identification and determination of the molecular weight of chemically unstable limonene hydroperoxides. An HPLC method for isolation of individual compounds in auto-oxidizedd-limonene is presented. Two different stationary phases were used in normal phase mode, cyanopropyl- and diamino-modified silica. The method described can be used for the isolation of individual contact allergens in sensitization experiment vehicles, such as petrolatum and olive oil. This makes it possible to study whether test compounds, such as hydroperoxides, are chemically stable during a sensitization experiment.
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  • 80
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Stationary phases ; TiO2 ZrO2 AlO3 ; Separation of basic compounds
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Chromatographic properties of silica, alumina, titania and zirconia have been investigated in normal phase mode in the separation of test mixtures of basic, neutral and acidic compounds. In contrast to silica the chromatographic behaviour revealed the basic properties of the alumina, titania and zirconia surfaces. Therefore, separation of basic compounds on these packings seems very promising. Lypophilic packings have been synthesized by modification of titania, zirconia and alumina with organosilanes and polymers and tested for the separation of basic compounds and proteins. High hydrolytic stability of the modified packings was observed during separations with strong alkali and acidic eluents.
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  • 81
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Column-switching technique ; Rabbit plasma ; Ampicillin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The purpose of this study was to develop a simple and fast analytical method for quantitation of ampicillin in rabbit plasma, suitable for analysis of large numbers of samples collected from experimental animals. The concentration of ampicillin in rabbit plasma was determined utilizing ion-pair reverse-phase high performance liquid chromatography (HPLC) with UV detection and a column switching technique. Plasma samples were treated with a perchloric acid solution to precipitate proteins and centrifuged to pellet the precipitated proteins. Cephalexin was used as an internal standard. The C18 precolumn was placed in the injector loop of the Rheodyne injector. Samples were injected with the injector in the load position and the precolumn was washed free from interfering compounds. When the injector was switched to the inject position, the mobile phase was passed through the precolumn taking relatively pure compounds onto the analytical column. The limit of quantitation was established to be 400 ng mL−1 of plasma. The standard curves were linear over the range of ampicillin concentrations assayed, 400 to 10,000 ng mL−1 of rabbit plasma, and had a mean regression coefficient of 0.9962 (±0.0043). Intra-day variability was determined using six replicates of controls (low and high) analyzed on a single assay. The percent of relative accuracy for low and high controls were 5.67 and 1.12, respectively. Inter-day variability was determined over a four day period analyzing replicates of each control. The percent of relative accuracy for low and high controls were 4.33 and 1.63, respectively.
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  • 82
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Size exclusion chromatography ; Pore structure ; Nitrogen sorptometry ; Zirconium oxide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The pore structures of zirconium oxide particles prepared by two different methods (PICA and Oil Emulsion processes) are compared. Nitrogen sorptometry and size exclusion chromatography are used to characterize the two different types of particles. Significant and unexpected differences were found in the accessible pore volumes and the bed packing densities of the two materials. The PICA material was found to have a higher totally included and totally excluded volume than would be normally expected. The chromatographic method provides results which are more useful for the application of these materials to separation sciences.
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  • 83
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 42 (1996), S. 401-404 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Hummel Dreyer method ; Sulfinpyrazone ; Bovine serum albumin ; Scatchard-Rosenthal plot
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Hummel-Dreyer method was used to study the binding of sulfinpyrazone to bovine serum albumin. The technique involves our newly developed internal calibration method with the elution pattern consisting of two positive peaks and with the integrator to evaluate the area of second peak. From the area of the second peak the numerical value ofL b (the amount of drug bound to protein) was calculated. Sctachard-Rosenthal plot was used to estimate the binding parametersn i (the number of binding sites) andk i (the binding constants). The results are compared with those obtained by equilibrium dialysis.
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  • 84
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Cu(II) dimethyldithiophosphate complex ; Malathion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A high-performance liquid chromatographic method has been developed for the quantitative determination of the organophosphorus pesticide malathion. The method is based on alkaline hydrolysis of malathion to dimthyldithiophosphate (DDTP) which is complexed with copper(II) and extracted in chlorofrom. The complex is injected onto a Hypersil ODS or Licrosorb sicolumn and eluted with acetonitrile:methanol:water (80∶10∶10 v:v:v) or chloroform. Detection is at 245 or 260 nm. A linear calibration curve was obtained for 24–240 μg ml−1 DDTP and a detection limit of 2.4–4.8 ng DDTP per injection. The method was applied to the analysis of malathion in commerical products after spraying on leaves. Extrction efficiency of malathion from leaves in acetone-water was also evlauted.
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  • 85
    ISSN: 1612-1112
    Schlagwort(e): Gas chromatography ; Column liquid chromatography ; Pesticide residues ; Surface water analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A monitoring programme based on gas chromatography (NPD, ECD) using MSD for confirmatory purposes and coupled-column liquid chromatography was applied to the analysis of pesticide residues in surface water from a predominantly agricultural area of Spain (Comunidad Valenciana). Samples analysed by means of enzyme-linked immunosorbent assay gave similar results to those obtained by GC (MSD) for the determination of total triazines. The test employed had the advantages of a simple test procedure, short analysis time and high confirmatory value. Nevertheless, the multiresidue character, accuracy and unequivocal identification of individual pesticide residues of GC (MSD) make this technique the most appropriate for environmental monitoring programmes. In this monitoring programme about 200 samples were analysed between 1993–1994. 27 different pesticides were detected in 91 of these samples. The pesticides more frequently detected were dimethoate, methidathion, endosulfan A and B, endosulfan sulphate and pirimicarb. The highest concentrations found were 39.9 μg L−1 of dimethoate, 10.6 of pirimicarb and 10.6 of methidathion.
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  • 86
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Liquid membrane extraction ; Chlorinated phenols ; Natural water samples
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A sample preparation method has been developed for the determination of chlorinated phenols in water. The method is based on a supported liquid membrane extraction system connected on-line to liquid chromatography with electrochemical detection. The supported liquid membrane technique utilizes a porous PTFE membrane. The membrane is impregnated with an organic solvent which forms a barrier between two aqueous phases and enables selective extraction. The technique can easily be coupled in a flow system. In this investigation five chlorinated phenols (1–5 chlorine atoms) were extracted from natural water samples. Extraction for 30 minutes resulted in detection limits of approximately 25 ng L−1.
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  • 87
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 42 (1996), S. 185-190 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Gradient elution of synthetic polymers ; Polystyrene and various styrene copolymers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Retention times in gradient liquid chromatography of synthetic polymers are often dependent on sample size. They increase with column load if the separation mechanism is governed by a solution process but decrease with increasing load if the mechanism is governed by adsorption. Since retention times independent of sample size are a prerequisite for peak identification as well as for the correct measurement of elution bands of samples with a broad distribution, measures to counteract sample-size effects deserve attention. Usually both solubility and adsorption are effective in gradient liquid chromatography of synthetic polymers. An appropriate balance of both effects is suitable for diminishing the influence of sample size on retention time of synthetic polymers. Ternary gradients allowing independent control of solubility and adsorption are promising.
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  • 88
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 42 (1996), S. 206-212 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Supercritical fluid extraction ; Carbamate pesticides ; Soils ; Cereal
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A method for the supercritical fluid extraction of carbamate pesticides (propoxur, aminocarb, carbaryl and methiocarb) from soil and cereal samples using CO2 is proposed. Extractions were at 378 bar and 54 °C. Analytes were determined in the extracts by HPLC with fluorescence detection after post-column derivatization. Recoveries from spiked soil ranged between 39.6 and 91.7%, depending on analyte and soil components. Lowest recoveries were from sandy soils. Aminocarb could not be recovered from any soil using CO2. Recovery of aminocarb from diatomaceous earth was improved by adding methanol to the extraction cell prior to SFE, but the effect was not observed in soil samples. Recoveries for propoxur and aminocarb from spiked wheat were about 75%, and only between 30–50% for aminocarb from corn and oats, and carbaryl from wheat. Fat was coextracted using CO2 and retained in the trap together with the analytes, however, appropriate rinsing solvent allowed on-line clean-up of the extract.
    Materialart: Digitale Medien
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  • 89
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Peak overlap ; Fluorescence detection ; H-Point standard additions method ; Water analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The H-Point Standard Additions Method (HPSAM) is proposed in order to resolve overlapping peaks in liquid chromatography by using a conventional fluorescence detector. The method uses as analytical signals the heights or the areas obtained at two previously selected emission wavelengths, and good results are obtained for highly overlapping peaks with highly overlapping fluorescence spectra. The principal benefits of the method are the ease of finding the required wavelengths, its insensitivity to changes in the retention time of the peak from one injection to another, and the possibility of using it in highly or only partially overlapping peaks. We have applied the method to the determination of phenol and cresols in water, resolving by the proposed method the overlapping peaks ofm- andp-cresol.
    Materialart: Digitale Medien
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  • 90
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Synthesis of new liquid phases ; Evaluation of new phases
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Three columns, mono-ol, butylphenyl and perfluorinated synthesized through the reaction of an olefin with a silica hydride intermediate, are evaluated for the separation of various model compounds. Two of the columns, mono-ol and perfluorinated, can operate in both the normal phase and reversed phase modes. Some effects of the hydride surface are detected, particularly for the perfluorinated phase. A four-component steroid mixture is tested on both the mono-ol and perfluorinated phases in the normal mode.
    Materialart: Digitale Medien
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  • 91
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Nitroaromatics ; Solid phase extraction ; Reduction and derivatization ; Fluorescence detection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A selective and sensitive method for determination of nitroaromatics is described. The analytes were reduced to corresponding primary amines with iron powder and then derivatized with fluram in citrate buffer to form pyrrolinones. The highly fluorescent pyrrolinones were isolated and preconcentrated by octadecylsilane solid phase extraction cartridge followed by reversed-phase, high-performance liquid chromatographic analysis. Detection was at 395–495 nm. Various aspects such as the reduction process, derivatization, solid phase extraction and chromatographic separation were optimized. Analysis time was relatively short due to a special design for successive reduction and preconcentration. Limits of detection for 3-nitrophenol, nitrobenzene, 4 and 2-nitrotoluene were less than 60, 12, 60 and 280 ng L−1 respectively.
    Materialart: Digitale Medien
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  • 92
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Yoghurt ; β-Galactosidase activity ; Glucose ; Galactose
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A new method has been developed for the HPLC determination of the activity of lactase from the microorganisms in yoghurt. The method is based upon the ability of β-galactosidase to hydrolyze lactose bonds in glucose and galactose. To determine such activity in yoghurt, 1 mL of yoghurt was diluted 1∶10 with 0.1 M phosphate buffer (pH 7) containing 2 % (w/v) lactose and 5 mM dithioerythritol as reducing agent; the mixture was the incubated and injected into the HPLC. Lactase activity remained high as long as the number of viable microorganisms did not fall below the minimum CFU·mL−1 (107). This method is more repeatable than conventional colorimetric determination, and may also be automated for routine analysis.
    Materialart: Digitale Medien
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  • 93
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Micellar vs. reversed phase separation ; Thiram in river water ; Dithiocarbamate fungicides
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary After a comparative study of conventional reversed phase and micellar liquid chromatography a method is described for the determination of the fungicide thiram in river water based on the use of micelles of CTAB. The method involves the extraction/concentration of thiram with a C18 cartridge followed by separation on a C18 reversed-phase column with a mobile phase of 20% (v/v) acetonitrile/0.01 M CTAB in phosphate buffer (pH-6.3), and detection at 254 nm. The method allows the determination of thiram in the presence of other water-soluble dithiocarbamate fungicides (nabam, ziram and ferbam) with a limit of detection of 36 pg mL−1 for a typical 20-fold preconcentration.
    Materialart: Digitale Medien
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  • 94
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 43 (1996), S. 654-658 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Thin-layer chromatography ; Ion-pairing ; Sulfonamides ; NH2-Modified stationary phase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Thin-layer and column high-performance liquid chromatography based on amino propyl-modified silica gel using aqueous and non-aqueous mobile phases containing organic modifier and cationic or anionic ion-pairing reagents were used for the determination ofR F andk values of some sulfonamides. The influence of the different organic modifiers and ion-pairing reagents on retention has been compared.
    Materialart: Digitale Medien
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  • 95
    Digitale Medien
    Digitale Medien
    Springer
    Chromatographia 42 (1996), S. 595-598 
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Non-porous, reversed phase packing ; Fast peptide separation ; Peptide mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary Kovasil-H, a new 1.5 μm, non-porous, silica-based, reversed phase packing was investigated for the peptide mapping of Bovin Serum Albumin (BSA) and Cytochrome c tryptic digests and for the quality control of trypsin. Using short columns (33–66 mm) and high temperature, very short analysis times and high sensitivities were attained. No special modification of commonly used liquid chromatographs are necessary for the application of this method.
    Materialart: Digitale Medien
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  • 96
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Caffeine ; Pharmaceutical formulations ; Micellar liquid chromatography
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary An HPLC procedure is described for the determination of caffeine in pharmaceutical preparations. A Spherisorb octadecylsilane ODS-2 C18 analytical column and spectrophotometric detection at 273 nm were used. The chromatographic behaviour of caffeine with different micellar eluents containing sodium dodecyl sulphate (SDS) is described. The determination of caffeine in pharmaceutical preparations was performed by use of a mobile phase containing 0.05 M sodium dodecylsulphate (SDS) and 1.5% propanol at pH7. At a 6.0 μg mL−1 concentration level the peak area and peak height repeatability were 2.6 and 2.4%, respectively. The application of the proposed method to the analysis of five pharmaceutical formulations, using peak heights as the dependent variable, gave recoveries between 85 and 104% of the values declared by the manufacturers. The proposed procedure for the determination of caffeine is rapid (15 min per sample), reliable and free from interferences.
    Materialart: Digitale Medien
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  • 97
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Leucovorin, methotrexate ; On-line, post-column UV irradiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary An improved, reversed-phase, high-performance liquid chromatography (HPLC) method with dual UV-fluorescence detection for simultaneous determination of the antifolate methotrexate, the folate leucovorin and their two main metabolites 7-hydroxymethotrexate and 5-methyltetrahydrofolate, respectively is presented. The fluorescence intensity of leucovorin could be significantly increased by on-line, post-column irradiation with UV at 254 nm thus lowering the limit of detection for leucovorin to 0.2 ng absolute at a signal-to-noise ratio 3∶1.
    Materialart: Digitale Medien
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  • 98
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Carbohydrates ; Anomer separations ; Post-column derivatization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary A sensitive post-column fluoresence method based on the reaction of reducing sugars with a fluorigenic reagent (benzamidine) under alkaline conditions and elevated temperature is described. Optimum reaction conditions suitable for the detection of fluorescent reaction products have been investigated. A porous graphitized carbon column (shandon Hypercarb) was employed as the chromatographic solid phase and elution was isocratic by an aqueous acetonitrile mixture. A special problem in sugar analysis is the tendendy of anomer formation giving doublet peaks. This tendency varies largely among the different sugars, and systematic studies on conditions that eliminate this problem were performed. Efficient and selective separations of a series of disaccharides were obtained using high temperatures and a low concentration of acetonitrile in the mobile phase. The detection limits (S/N=3) with an injection volume of 20 μL ranged from 10–60 picomoles. Linear calibration graphs were obtained in the 2–20 μM concentration range (r 2〉0.99). A relative standard deviation between 1–3% (n=6) demonstrated good precision of the developed system.
    Materialart: Digitale Medien
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  • 99
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Chromogenic reagent ; Chloroformate ; Pre-column derivatization ; Amino acids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The chromogenic reagent 4-phenylazobenzyloxycarbonyl chloride (PAZ-Cl) was used for the automated pre-column derivatization of amino acids (AAs) at ambient temperature followed by liquid chromatographic separation of the derivatives formed. Since in previous investigations it was realised that the selectivity of a C-18 stationary phase decreased after 40 injections of PAZ-AAs the suitability of 12 reversedphases distinguished by silica modification, carbon content, and particle characteristics was tested. Among these columns only a polymer-coated C-18 stationary phase furnished satisfactory repeatability of retention times for 250 analyses.
    Materialart: Digitale Medien
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  • 100
    ISSN: 1612-1112
    Schlagwort(e): Column liquid chromatography ; Ruthenium diimine complexes ; Inorganic synthesis ; Kinetics monitoring
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The synthesis of the complex [(bpy)2Ru(Im)2]2+ (bpy=2,2′-bipyridine; Im=imidazole) has been monitored by reversed-phase HPLC. The analytical results obtained during the reaction have shown that it is feasible to identify and isolate the [(bpy)2RuIm(H2O)]2+ complex as a reaction intermediate. The optimization of the synthetic procedures for these two species has been established and the compounds have been obtained in high purity. The use of HPLC has enabled complete analytical control of the synthesis of the [(bpy)2RuL2]2+ class of compounds, enabling the identification of reaction intermediates.
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