ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Chromogenic reagent
;
Chloroformate
;
Pre-column derivatization
;
Amino acids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The chromogenic reagent 4-phenylazobenzyloxycarbonyl chloride (PAZ-Cl) was used for the automated pre-column derivatization of amino acids (AAs) at ambient temperature followed by liquid chromatographic separation of the derivatives formed. Since in previous investigations it was realised that the selectivity of a C-18 stationary phase decreased after 40 injections of PAZ-AAs the suitability of 12 reversedphases distinguished by silica modification, carbon content, and particle characteristics was tested. Among these columns only a polymer-coated C-18 stationary phase furnished satisfactory repeatability of retention times for 250 analyses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02270994
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