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  • General Chemistry  (77,717)
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  • Triticum aestivum
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  • 1
    Publication Date: 2024-02-26
    Description: Identifcation of ontogenetic age classes plays an important role in the felds of zoology, palaeontology and archaeology, where accurate age classifcations of (sub)fossil remains are a crucial component for the reconstruction \nof past life. Textural ageing\xe2\x80\x94the identifcation of age-related bone surface textures\xe2\x80\x94provides a size-independent \nmethod for age assessment of vertebrate material. However, most of the work so far is limited to qualitative results. \nWhile qualitative approaches provide helpful insights on textural ageing patterns, they are heavily subject to observer \nbias and fall short of quantitative data relevant for detailed statistical analyses and cross-comparisons. Here, we present a pilot study on the application of 3D surface digital microscopy to quantify bone surface textures on the long \nbones of the grey heron (Ardea cinerea) and the Canada goose (Branta canadensis) using internationally verifed \nroughness parameters. Using a standardised measuring protocol, computed roughness values show a strong correlation with qualitative descriptions of textural patterns. Overall, higher roughness values correspond to increased numbers of grooves and pits and vice versa. Most of the roughness parameters allowed distinguishing between diferent \nontogenetic classes and closely followed the typical sigmoidal animal growth curve. Our results show that bone \ntexture quantifcation is a feasible approach to identifying ontogenetic age classes.
    Keywords: General Physics and Astronomy ; General Environmental Science ; General Biochemistry ; Genetics and Molecular Biology ; General Materials Science ; General Chemistry ; Roughness ; Surfaces ; Taphonomy ; Topography ; Bone ; Ontogeny ; Digital microscopy
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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  • 2
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2023-09-11
    Keywords: Laurus nobilis ; medicinal plant ; mobile genomic elements ; germplasm characterization ; Mediterranean region ; chloroplast ; Ipomoea batatas ; simple sequence repeat ; sweet potato ; plant germplasm ; landrace rice ; fat-soluble nutraceuticals ; β-sitosterol ; genetic variability ; cluster analysis ; Solanum melongena ; germplasm ; Greece ; islands ; mainland ; phenotyping ; genotyping ; mineral composition ; landraces ; genetic diversity ; genetic structure ; Panax ginseng ; Triticum turgidum ; HMW glutenins ; LMW glutenins ; gluten quality ; non-allelic interactions ; combined analysis ; evolution ; genetic resources ; Zea mays ; Blumeria graminis ; disomic addition line ; molecular cytogenetics ; wheat ; Psathyrostachys huashanica ; natural variation ; maize ; root length ; domestication selection ; ZmMADS60 gene ; genetic basis ; GWAS ; eating and cooking qualities ; rice ; genetic variation ; eggplant ; cropping condition ; yield ; agro-morphological characterization ; chili pepper ; gene bank ; molecular markers ; morphological descriptor ; DArTseq markers ; GBS ; Triticum aestivum ; starch ; SNP ; InDel ; CAPS ; intron-loss ; NGS ; phosphorus use efficiency ; phosphorus ; proteomics ; grain hardness ; PIN ; kernel texture ; triticum ; SKCS ; Cucumis sativum ; downy mildew ; genetics ; inheritance ; oomycetes ; resistance ; rice genotypes ; blast resistant genotype ; genotypic coefficient of variation (GCV) ; phenotypic coefficient of variation (PCV) ; heritability values ; DArT SNP markers ; early maturity ; heat and drought tolerance ; salt stress ; nitrogen metabolism ; oxidative stress response ; G6PDH ; GDH ; GS/GOGAT ; Triticum aestivum L. ; γ-gliadins ; Gli-B1 ; polymorphism ; PCR analysis ; ginseng ; genetic composition ; SSR ; fonio ; fonio millet ; white fonio ; Digitaria exilis ; agro morphological descriptors ; phenotypic diversity ; neglected and underutilized species (NUS) ; genetic improvement ; catechin ; phytochemicals ; targeted-oriented core collection ; tea germplasm ; agronomic performance ; correlation analysis ; malawi ; pigeonpea ; yield stability ; bermudagrass ; forage breeding ; genetic parameters ; genotype by harvest interaction ; Tifton 85 ; accessions ; descriptors ; anthracnose ; Colletotrichum lentis ; disease screening ; lentil ; plant resistance ; tall wild pea ; Pisum sativum subsp. elatius ; neoplasm ; pea weevil ; Bruchus pisorum ; expressivity ; Africa ; cowpea ; microsatellites ; Aegilops ; triticale ; leaf rust ; stripe rust ; yellow rust ; Puccinia ; drought ; Phaseolus vulgaris L. ; plant breeding ; rhizobia ; stress ; n/a ; bic Book Industry Communication::G Reference, information & interdisciplinary subjects::GP Research & information: general ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences
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  • 3
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2024-03-28
    Description: This reprint represents a general view of what and how the research on plants at the molecular level (genetics, genomics, transcriptomics, proteomics, metabolomics, and so on) contributes to a good equilibrium among human needs, food security, and future strategies for mitigating the effects of global climate changes. Now more than ever, it is critical to understand the genetics and evolution of the gene mechanisms and the networks of different molecular pathways acting on plant abiotic stress tolerance in order to find new solutions for modern agricultural problems.This reprint is full of technical and specialized terms and, for this reason, its target audience is scientists and students trained in plant functional genomics, breeding, agronomy, and genetics. It is an exciting virtual tour through plant molecular responses to various environmental stresses, and new ideas and applications will be derived.
    Keywords: environmental stress ; seed development ; site II element ; HSFA2 ; TT2/ MYB5-MBW complex ; triacontanol ; drought ; rice ; aquaporins ; PIP1,1, PIP1,2, PIP2,4 and PIP2,5 genes ; abiotic stress ; biotic stress ; crop improvement ; HD-ZIP ; plant development ; cold ; stress ; differentially expressed genes ; transcriptome ; transcription factors ; in silico ; Cis-regulatory elements ; gene transcription ; trehalose-6-phosphate phosphatase ; wheat ; early heat stress ; Triticum aestivum ; heat tolerance ; VRN ; PPD ; photoperiod ; Rosmarinus officinalis Lour. ; suspension cells ; MeJA ; antioxidant enzymes ; RNA-seq ; qRT-PCR ; durum wheat ; osmotic adjustment ; QTL ; climate change ; drought tolerance ; crop modelling ; expression profiles ; field trials ; TdDRF1 gene ; Wdhn13 ; n/a ; thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
    Language: English
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  • 4
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2023-09-11
    Description: 10th Anniversary of Plants—Recent Advances and Perspectives is a scientific paper collection specially published on the anniversary of Plants. Covering all major areas of plant science, it is a valuable guide through current achievements and future discoveries in this scientific field.
    Keywords: Secale cereale ; Secale montanum ; Secale strictum ; QTL mapping ; molecular marker ; self-incompatibility ; fertility ; seed set ; abiotic stress ; cell homeostasis ; heterologous host synthetic approach ; terpenophenolics ; brown spot ; ACT ; fungus culture filtrate ; mycotoxin ; fruit development ; fruit gauge ; VPD ; Mangifera indica ; cell division ; cell expansion ; ripening ; pulegone ; isomenthone ; menthone ; thymol ; p-cymene ; chemotypes ; seasonal variation ; enantiomeric distribution ; label-free proteomics ; Panax ginseng ; ginsenosides ; cytochrome p450 ; UDP-glycosyltransferase ; MEP pathway ; MVA pathway ; TCA/acetone ; methanol/chloroform ; endophytes ; foliar pathogens ; pathogenicity ; taxonomy ; Thymus vulgaris ; Crithmum maritimum ; leather artifacts ; essential oils ; anti-bacterial activity ; Euphorbia dendroides L. ; aerial parts ; polyphenols ; antioxidant activity ; anti-inflammatory activity ; toxicity ; calcium oxalate crystals ; colleter ; extrafloral nectaries ; resin gland ; bud protection ; plant-environment interaction ; carbohydrate metabolism ; microarray ; crop ; rice ; productivity ; endosperm ; geometry ; morphology ; seed shape ; Vitaceae ; exDNA ; environmental DNA ; DNA sensing ; self-DNA inhibition ; autotoxicity ; plant response ; DAMP ; PAMP ; EDAP ; climate change ; food security ; Mediterranean countries ; sustainable exploitation ; phytogenetic resources ; candidate gene ; quantitative trait locus ; recombinant inbred line ; soybean drought tolerance ; weighted drought coefficient ; antioxidants ; biostimulants ; biotic stress ; GABA ; metabolism ; phytohormones ; reactive oxygen species ; signaling ; tricarboxylic acid cycle ; bacterial functions ; co-presence networks ; metagenomics ; microbial ecology ; plant domestication ; trace element ; plant nutrient ; salinity ; antioxidant defense system ; glyoxalase system ; biochar ; licorice ; soil enzymes ; nutrients ; root system ; ALS ; BCAA ; low oxygen ; flooding ; AIP1 ; Eucommia ulmoides Oliver ; trait variations ; probability grading ; quantitative traits ; planting models ; leaves ; cytokinin ; TD-K ; thidiazuron ; INCYDE ; CPPU ; isopentenyl transferase ; IPT ; cytokinin oxidase/dehydrogenase ; CKX ; wheat ; barley ; yield ; cucumber ; QTL-seq ; SNP markers ; white immature fruit skin color ; ecological costs ; germination models ; herbicide resistance ; hydrotime ; target-site resistance ; hydrogen peroxide ; sodium hypochlorite ; generalized regression neural network ; genetic algorithm ; scarification ; seed dormancy ; plant tissue culture ; foliar descriptors ; leaf area ; models ; vine leaves ; Olea europaea L. ; olive ; genotype by sequencing (GBS) ; single-nucleotide polymorphism (SNP) ; whole-genome sequencing (WGS) ; reference genome ; plastid markers ; DNA barcoding ; ISSR markers ; Egyptian barley ; agro-morphological traits ; cluster analysis ; genetic variation ; biplot ; drought stress ; drying processes ; mathematical model ; plant hydric stress tolerance ; rate of weight loss ; RWLMod ; water evaporation ; photosynthesis ; elevated CO2 ; Rubisco ; electron transport ; light ; diurnal cycle ; sexual propagation ; cold stratification ; in situ ; ex situ ; plant endemism ; Morocco ; biodiversity ; ex-situ conservation ; protocols ; germplasm ; forest berries ; brushing ; lettuce ; chicory ; phytochemicals ; antioxidant capacity ; Ziziphus lotus ; phenolics ; SH-SY5Y cell line ; chromatography ; Koelreuteria paniculata ; dry ethanol extracts ; GC-MS analysis ; chemical compounds ; antitumor and antimicrobial activities ; medicinal plant ; bioactive compounds ; plant-derived secondary metabolites (PDSM) ; cell suspension culture (CSC) ; bioreactor engineering ; apple ; Golden Delicious ; Top Red ; fruitlet thinners ; light reactions ; electron transport rate ; photoprotective mechanism ; state transitions ; PSII repair cycle ; vegetation structure ; environmental variables ; PC-ORD ; plant community assembly ; Himalaya ; allopolyploidy ; interspecific hybridization ; unreduced gametes ; cytological diploidization ; genomic changes ; root length ; root/shoot ratio ; specific root length ; Saragolle Lucana ; seed coating ; heavy metals ; evolution ; hyperaccumulation ; black chokeberry (Aronia melanocarpa) ; anthocyanin stability ; herbs ; co-pigmentation ; color stability ; functional foods/beverages ; biotechnological tools ; ethnomedicine ; in vitro culture ; genetic improvement ; pollen ; tip growth ; calcium ; calcium dependent protein kinase ; Rho Guanine Dissociation Inhibitor ; ROP GTPase ; RhoGDI displacement factor ; polarity ; guar ; gene expression ; qRT-PCR ; RNA-Seq ; salt stress ; salt tolerance ; stress ; transcriptome ; D-tagatose ; IFP48 ; induced resistance ; sweet immunity ; sugar-enhanced defense ; Plasmopara viticola ; Botrytis cinerea ; Vitis vinifera ; human diet ; edible wild plants ; Plantago coronopus L. ; Rumex acetosa L. ; Cichorium intybus L. ; Artemisia dracunculus L. ; phytochemistry ; anti-inflammatory properties ; stem photosynthesis ; hydraulic recovery ; soaking ; X-ray micro-CT ; bark water uptake ; embolism ; genetic resources ; Solanaceae ; Cucumis ; Lactuca ; diversity ; vegetables ; genebank ; essential oil ; iNOS ; interleukin ; lavenders ; NF-κB ; glycosyltransferases ; ER-Golgi trafficking ; mechanism of protein sorting ; COPI and COPII complexes ; sequences and motifs involved in trafficking ; Arabidopsis ; gene regulation ; protein-protein interaction ; transcription factor ; WRI1 ; TCP20 ; lipases ; lipid metabolism ; plant-environment interactions ; reproductive development ; vegetative development ; Urtica dioica ; soilless systems ; cultivated nettle ; stress factors ; functional properties ; preharvest sprouting ; MKK3 ; maternal and paternal expressed genes ; imprinted genes ; polycomb repressive complex 2 ; mRNA processing bodies ; ribonucleic binding proteins ; monosomes ; ethylene ; elicitors ; fruit ripening ; ACC synthase/oxidase ; GC-MS ; polyamines ; Vigna genus ; introgression ; hybridisation ; phylogeny ; de novo domestication ; feralisation ; novel ecosystems ; complex networks ; tree communities ; Lantana camara ; Prosopis juliflora ; ascorbic acid ; genetic diversity ; molecular markers ; aquaculture pond sediment ; recovery ; Triticum aestivum ; chlorophyll fluorescence ; wheat grass juice quality ; UV-B radiation ; olive tree ; metabolomic ; phenolic profile ; lipophilic profile ; ecophysiology ; environment ; arbuscular mycorrhizal symbiosis ; comparative transcriptomics ; Arum-type ; Paris-type ; Solanum lycopersicum ; Rhizophagus irregularis ; Gigaspora margarita ; Camelina sativa ; semi-arid lands ; biofuel feedstock ; biodiesel ; renewable diesel ; crop breeding ; transgenesis ; genome editing ; Xanthomonas euvesicatoria ; host associate factor ; comparative genomics ; Cannabis sativa L. ; chemovars ; secondary metabolites ; trichomes ; residual by-products ; biogeography ; cardioid ; islands ; geometric models ; Mediterranean flora ; Silene ; super-ellipse ; abscisic acid ; aromatic herb ; ascorbate-glutathione cycle ; jasmonic acid ; lipoic acid ; oxidative stress ; salicylic acid ; Salvia officinalis ; drought ; state of stress ; tolerance ; avoidance ; stress survival ; amino acids ; nitrate reductase ; glutamine synthetase ; plants mycorrhized ; dark septate ; Daphne genkwa ; Thymelaeaceae ; flavonoids ; design of experiments ; blooming stages ; germination stimulant ; witchweed ; methyl phenlactonoates (MPs) ; Nijmegen-1 ; weed ; plant development ; vasculature ; leaf traces ; structure ; microtomography ; Euphorbiaceae ; in vitro crop ; gamma radiation ; ionizing radiation ; mutants ; Fumaria scheleicheri Soy. Will. ; isoquinoline alkaloids ; HPLC-DAD ; in vitro anti-cholinesterase ; cytotoxic ; antioxidant ; ABC model ; hop ; transcription factors ; type-II MADS box ; type-I MADS-box ; AFLP ; carpological traits ; genetic structure ; molecular systematics ; plastid phylogeny ; Valerianaceae ; auxins ; embryogenic calli ; HPLC ; IAA ; immunohistochemistry ; deficit irrigation ; grape quality ; phenology ; plant diseases ; bacterium ; symptoms ; molecular classification ; common juniper ; common larch ; Cupressaceae ; Pinaceae ; SPME-GC-MS ; volatile compounds ; herbicidal activity ; weed control ; trait association ; GCV ; genetic variability ; genetic advance ; heritability ; PCV ; Cicer arietinum L. ; gold nanoparticles ; carbon nanotubes ; ATR-FTIR spectroscopy ; machine learning techniques ; principal component analysis ; support vector machine classification ; citrus ; melanose ; Diaporthe citri ; epidemiology ; symptomatology ; Pseudomonas cannabina pv. alisalensis ; resistance-nodulation-cell division transporter ; type-three secretion system ; phytoalexin ; brassinin ; glucosinolate ; cabbage ; flowering ; juvenile traits ; genetic stability ; flow cytometry ; somaclonal variation ; thorniness ; carbohydrates ; protein ; lipids ; fatty acids ; minerals ; plastome ; Plicosepalus acaciae ; Plicosepalus curviflorus ; loranthaceae ; mistletoe ; phylogenetic relationship ; plastome structure ; comparative analysis ; magnetic resonance imaging ; Solanum tuberosum ; multi-exponential transverse relaxation ; water stress ; broccoli ; human nutrition ; improved health ; melatonin ; postharvest ; apricot ; pollen tube ; pollination ; Prunus armeniaca ; S-alleles ; Populus ; hexokinase ; sucrose metabolism ; sugar signaling ; stress and defense ; centres of origin ; crop wild relatives ; crop domestication ; cryopreservation ; conservation ; in vitro storage ; ecosystem restoration ; plant breeding ; acidification ; alkalinisation ; bud burst ; freezing ; Malus domestica ; pH ; Picea abies ; Pinus cembra ; histone modification ; Taraxacum kok-saghyz ; natural rubber ; high light stress ; singlet oxygen ; signalling ; GPX5 ; beta cyclocitral ; acrolein ; glutathione peroxidase ; carbonyl ; transcription ; SLIM1 transcription factor ; sulfur deficiency ; Arabidopsis thaliana ; sulfate transporter ; sulfate assimilation ; stress tolerance ; LRR-RLK receptors ; dodders ; parasitic plants ; proteomics ; virus vertical transmission ; CMV-Fny strain ; pseudorecombinant virus ; chimeric virus ; infection rate ; seed-growth tests ; electron microscopy ; circular dichroism spectroscopy ; viral assembly ; Adiantetea capilli-veneris ; demographic analysis ; ecology ; IUCN ; plant conservation ; phytosociology ; rupicolous habitat ; n/a ; bic Book Industry Communication::G Reference, information & interdisciplinary subjects::GP Research & information: general ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences
    Language: English
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  • 5
    facet.materialart.
    Unknown
    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2023-09-11
    Description: 10th Anniversary of Plants—Recent Advances and Perspectives is a scientific paper collection specially published on the anniversary of Plants. Covering all major areas of plant science, it is a valuable guide through current achievements and future discoveries in this scientific field.
    Keywords: Secale cereale ; Secale montanum ; Secale strictum ; QTL mapping ; molecular marker ; self-incompatibility ; fertility ; seed set ; abiotic stress ; cell homeostasis ; heterologous host synthetic approach ; terpenophenolics ; brown spot ; ACT ; fungus culture filtrate ; mycotoxin ; fruit development ; fruit gauge ; VPD ; Mangifera indica ; cell division ; cell expansion ; ripening ; pulegone ; isomenthone ; menthone ; thymol ; p-cymene ; chemotypes ; seasonal variation ; enantiomeric distribution ; label-free proteomics ; Panax ginseng ; ginsenosides ; cytochrome p450 ; UDP-glycosyltransferase ; MEP pathway ; MVA pathway ; TCA/acetone ; methanol/chloroform ; endophytes ; foliar pathogens ; pathogenicity ; taxonomy ; Thymus vulgaris ; Crithmum maritimum ; leather artifacts ; essential oils ; anti-bacterial activity ; Euphorbia dendroides L. ; aerial parts ; polyphenols ; antioxidant activity ; anti-inflammatory activity ; toxicity ; calcium oxalate crystals ; colleter ; extrafloral nectaries ; resin gland ; bud protection ; plant-environment interaction ; carbohydrate metabolism ; microarray ; crop ; rice ; productivity ; endosperm ; geometry ; morphology ; seed shape ; Vitaceae ; exDNA ; environmental DNA ; DNA sensing ; self-DNA inhibition ; autotoxicity ; plant response ; DAMP ; PAMP ; EDAP ; climate change ; food security ; Mediterranean countries ; sustainable exploitation ; phytogenetic resources ; candidate gene ; quantitative trait locus ; recombinant inbred line ; soybean drought tolerance ; weighted drought coefficient ; antioxidants ; biostimulants ; biotic stress ; GABA ; metabolism ; phytohormones ; reactive oxygen species ; signaling ; tricarboxylic acid cycle ; bacterial functions ; co-presence networks ; metagenomics ; microbial ecology ; plant domestication ; trace element ; plant nutrient ; salinity ; antioxidant defense system ; glyoxalase system ; biochar ; licorice ; soil enzymes ; nutrients ; root system ; ALS ; BCAA ; low oxygen ; flooding ; AIP1 ; Eucommia ulmoides Oliver ; trait variations ; probability grading ; quantitative traits ; planting models ; leaves ; cytokinin ; TD-K ; thidiazuron ; INCYDE ; CPPU ; isopentenyl transferase ; IPT ; cytokinin oxidase/dehydrogenase ; CKX ; wheat ; barley ; yield ; cucumber ; QTL-seq ; SNP markers ; white immature fruit skin color ; ecological costs ; germination models ; herbicide resistance ; hydrotime ; target-site resistance ; hydrogen peroxide ; sodium hypochlorite ; generalized regression neural network ; genetic algorithm ; scarification ; seed dormancy ; plant tissue culture ; foliar descriptors ; leaf area ; models ; vine leaves ; Olea europaea L. ; olive ; genotype by sequencing (GBS) ; single-nucleotide polymorphism (SNP) ; whole-genome sequencing (WGS) ; reference genome ; plastid markers ; DNA barcoding ; ISSR markers ; Egyptian barley ; agro-morphological traits ; cluster analysis ; genetic variation ; biplot ; drought stress ; drying processes ; mathematical model ; plant hydric stress tolerance ; rate of weight loss ; RWLMod ; water evaporation ; photosynthesis ; elevated CO2 ; Rubisco ; electron transport ; light ; diurnal cycle ; sexual propagation ; cold stratification ; in situ ; ex situ ; plant endemism ; Morocco ; biodiversity ; ex-situ conservation ; protocols ; germplasm ; forest berries ; brushing ; lettuce ; chicory ; phytochemicals ; antioxidant capacity ; Ziziphus lotus ; phenolics ; SH-SY5Y cell line ; chromatography ; Koelreuteria paniculata ; dry ethanol extracts ; GC-MS analysis ; chemical compounds ; antitumor and antimicrobial activities ; medicinal plant ; bioactive compounds ; plant-derived secondary metabolites (PDSM) ; cell suspension culture (CSC) ; bioreactor engineering ; apple ; Golden Delicious ; Top Red ; fruitlet thinners ; light reactions ; electron transport rate ; photoprotective mechanism ; state transitions ; PSII repair cycle ; vegetation structure ; environmental variables ; PC-ORD ; plant community assembly ; Himalaya ; allopolyploidy ; interspecific hybridization ; unreduced gametes ; cytological diploidization ; genomic changes ; root length ; root/shoot ratio ; specific root length ; Saragolle Lucana ; seed coating ; heavy metals ; evolution ; hyperaccumulation ; black chokeberry (Aronia melanocarpa) ; anthocyanin stability ; herbs ; co-pigmentation ; color stability ; functional foods/beverages ; biotechnological tools ; ethnomedicine ; in vitro culture ; genetic improvement ; pollen ; tip growth ; calcium ; calcium dependent protein kinase ; Rho Guanine Dissociation Inhibitor ; ROP GTPase ; RhoGDI displacement factor ; polarity ; guar ; gene expression ; qRT-PCR ; RNA-Seq ; salt stress ; salt tolerance ; stress ; transcriptome ; D-tagatose ; IFP48 ; induced resistance ; sweet immunity ; sugar-enhanced defense ; Plasmopara viticola ; Botrytis cinerea ; Vitis vinifera ; human diet ; edible wild plants ; Plantago coronopus L. ; Rumex acetosa L. ; Cichorium intybus L. ; Artemisia dracunculus L. ; phytochemistry ; anti-inflammatory properties ; stem photosynthesis ; hydraulic recovery ; soaking ; X-ray micro-CT ; bark water uptake ; embolism ; genetic resources ; Solanaceae ; Cucumis ; Lactuca ; diversity ; vegetables ; genebank ; essential oil ; iNOS ; interleukin ; lavenders ; NF-κB ; glycosyltransferases ; ER-Golgi trafficking ; mechanism of protein sorting ; COPI and COPII complexes ; sequences and motifs involved in trafficking ; Arabidopsis ; gene regulation ; protein-protein interaction ; transcription factor ; WRI1 ; TCP20 ; lipases ; lipid metabolism ; plant-environment interactions ; reproductive development ; vegetative development ; Urtica dioica ; soilless systems ; cultivated nettle ; stress factors ; functional properties ; preharvest sprouting ; MKK3 ; maternal and paternal expressed genes ; imprinted genes ; polycomb repressive complex 2 ; mRNA processing bodies ; ribonucleic binding proteins ; monosomes ; ethylene ; elicitors ; fruit ripening ; ACC synthase/oxidase ; GC-MS ; polyamines ; Vigna genus ; introgression ; hybridisation ; phylogeny ; de novo domestication ; feralisation ; novel ecosystems ; complex networks ; tree communities ; Lantana camara ; Prosopis juliflora ; ascorbic acid ; genetic diversity ; molecular markers ; aquaculture pond sediment ; recovery ; Triticum aestivum ; chlorophyll fluorescence ; wheat grass juice quality ; UV-B radiation ; olive tree ; metabolomic ; phenolic profile ; lipophilic profile ; ecophysiology ; environment ; arbuscular mycorrhizal symbiosis ; comparative transcriptomics ; Arum-type ; Paris-type ; Solanum lycopersicum ; Rhizophagus irregularis ; Gigaspora margarita ; Camelina sativa ; semi-arid lands ; biofuel feedstock ; biodiesel ; renewable diesel ; crop breeding ; transgenesis ; genome editing ; Xanthomonas euvesicatoria ; host associate factor ; comparative genomics ; Cannabis sativa L. ; chemovars ; secondary metabolites ; trichomes ; residual by-products ; biogeography ; cardioid ; islands ; geometric models ; Mediterranean flora ; Silene ; super-ellipse ; abscisic acid ; aromatic herb ; ascorbate-glutathione cycle ; jasmonic acid ; lipoic acid ; oxidative stress ; salicylic acid ; Salvia officinalis ; drought ; state of stress ; tolerance ; avoidance ; stress survival ; amino acids ; nitrate reductase ; glutamine synthetase ; plants mycorrhized ; dark septate ; Daphne genkwa ; Thymelaeaceae ; flavonoids ; design of experiments ; blooming stages ; germination stimulant ; witchweed ; methyl phenlactonoates (MPs) ; Nijmegen-1 ; weed ; plant development ; vasculature ; leaf traces ; structure ; microtomography ; Euphorbiaceae ; in vitro crop ; gamma radiation ; ionizing radiation ; mutants ; Fumaria scheleicheri Soy. Will. ; isoquinoline alkaloids ; HPLC-DAD ; in vitro anti-cholinesterase ; cytotoxic ; antioxidant ; ABC model ; hop ; transcription factors ; type-II MADS box ; type-I MADS-box ; AFLP ; carpological traits ; genetic structure ; molecular systematics ; plastid phylogeny ; Valerianaceae ; auxins ; embryogenic calli ; HPLC ; IAA ; immunohistochemistry ; deficit irrigation ; grape quality ; phenology ; plant diseases ; bacterium ; symptoms ; molecular classification ; common juniper ; common larch ; Cupressaceae ; Pinaceae ; SPME-GC-MS ; volatile compounds ; herbicidal activity ; weed control ; trait association ; GCV ; genetic variability ; genetic advance ; heritability ; PCV ; Cicer arietinum L. ; gold nanoparticles ; carbon nanotubes ; ATR-FTIR spectroscopy ; machine learning techniques ; principal component analysis ; support vector machine classification ; citrus ; melanose ; Diaporthe citri ; epidemiology ; symptomatology ; Pseudomonas cannabina pv. alisalensis ; resistance-nodulation-cell division transporter ; type-three secretion system ; phytoalexin ; brassinin ; glucosinolate ; cabbage ; flowering ; juvenile traits ; genetic stability ; flow cytometry ; somaclonal variation ; thorniness ; carbohydrates ; protein ; lipids ; fatty acids ; minerals ; plastome ; Plicosepalus acaciae ; Plicosepalus curviflorus ; loranthaceae ; mistletoe ; phylogenetic relationship ; plastome structure ; comparative analysis ; magnetic resonance imaging ; Solanum tuberosum ; multi-exponential transverse relaxation ; water stress ; broccoli ; human nutrition ; improved health ; melatonin ; postharvest ; apricot ; pollen tube ; pollination ; Prunus armeniaca ; S-alleles ; Populus ; hexokinase ; sucrose metabolism ; sugar signaling ; stress and defense ; centres of origin ; crop wild relatives ; crop domestication ; cryopreservation ; conservation ; in vitro storage ; ecosystem restoration ; plant breeding ; acidification ; alkalinisation ; bud burst ; freezing ; Malus domestica ; pH ; Picea abies ; Pinus cembra ; histone modification ; Taraxacum kok-saghyz ; natural rubber ; high light stress ; singlet oxygen ; signalling ; GPX5 ; beta cyclocitral ; acrolein ; glutathione peroxidase ; carbonyl ; transcription ; SLIM1 transcription factor ; sulfur deficiency ; Arabidopsis thaliana ; sulfate transporter ; sulfate assimilation ; stress tolerance ; LRR-RLK receptors ; dodders ; parasitic plants ; proteomics ; virus vertical transmission ; CMV-Fny strain ; pseudorecombinant virus ; chimeric virus ; infection rate ; seed-growth tests ; electron microscopy ; circular dichroism spectroscopy ; viral assembly ; Adiantetea capilli-veneris ; demographic analysis ; ecology ; IUCN ; plant conservation ; phytosociology ; rupicolous habitat ; n/a ; bic Book Industry Communication::G Reference, information & interdisciplinary subjects::GP Research & information: general ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences
    Language: English
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  • 6
    facet.materialart.
    Unknown
    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2023-09-11
    Description: 10th Anniversary of Plants—Recent Advances and Perspectives is a scientific paper collection specially published on the anniversary of Plants. Covering all major areas of plant science, it is a valuable guide through current achievements and future discoveries in this scientific field.
    Keywords: Secale cereale ; Secale montanum ; Secale strictum ; QTL mapping ; molecular marker ; self-incompatibility ; fertility ; seed set ; abiotic stress ; cell homeostasis ; heterologous host synthetic approach ; terpenophenolics ; brown spot ; ACT ; fungus culture filtrate ; mycotoxin ; fruit development ; fruit gauge ; VPD ; Mangifera indica ; cell division ; cell expansion ; ripening ; pulegone ; isomenthone ; menthone ; thymol ; p-cymene ; chemotypes ; seasonal variation ; enantiomeric distribution ; label-free proteomics ; Panax ginseng ; ginsenosides ; cytochrome p450 ; UDP-glycosyltransferase ; MEP pathway ; MVA pathway ; TCA/acetone ; methanol/chloroform ; endophytes ; foliar pathogens ; pathogenicity ; taxonomy ; Thymus vulgaris ; Crithmum maritimum ; leather artifacts ; essential oils ; anti-bacterial activity ; Euphorbia dendroides L. ; aerial parts ; polyphenols ; antioxidant activity ; anti-inflammatory activity ; toxicity ; calcium oxalate crystals ; colleter ; extrafloral nectaries ; resin gland ; bud protection ; plant-environment interaction ; carbohydrate metabolism ; microarray ; crop ; rice ; productivity ; endosperm ; geometry ; morphology ; seed shape ; Vitaceae ; exDNA ; environmental DNA ; DNA sensing ; self-DNA inhibition ; autotoxicity ; plant response ; DAMP ; PAMP ; EDAP ; climate change ; food security ; Mediterranean countries ; sustainable exploitation ; phytogenetic resources ; candidate gene ; quantitative trait locus ; recombinant inbred line ; soybean drought tolerance ; weighted drought coefficient ; antioxidants ; biostimulants ; biotic stress ; GABA ; metabolism ; phytohormones ; reactive oxygen species ; signaling ; tricarboxylic acid cycle ; bacterial functions ; co-presence networks ; metagenomics ; microbial ecology ; plant domestication ; trace element ; plant nutrient ; salinity ; antioxidant defense system ; glyoxalase system ; biochar ; licorice ; soil enzymes ; nutrients ; root system ; ALS ; BCAA ; low oxygen ; flooding ; AIP1 ; Eucommia ulmoides Oliver ; trait variations ; probability grading ; quantitative traits ; planting models ; leaves ; cytokinin ; TD-K ; thidiazuron ; INCYDE ; CPPU ; isopentenyl transferase ; IPT ; cytokinin oxidase/dehydrogenase ; CKX ; wheat ; barley ; yield ; cucumber ; QTL-seq ; SNP markers ; white immature fruit skin color ; ecological costs ; germination models ; herbicide resistance ; hydrotime ; target-site resistance ; hydrogen peroxide ; sodium hypochlorite ; generalized regression neural network ; genetic algorithm ; scarification ; seed dormancy ; plant tissue culture ; foliar descriptors ; leaf area ; models ; vine leaves ; Olea europaea L. ; olive ; genotype by sequencing (GBS) ; single-nucleotide polymorphism (SNP) ; whole-genome sequencing (WGS) ; reference genome ; plastid markers ; DNA barcoding ; ISSR markers ; Egyptian barley ; agro-morphological traits ; cluster analysis ; genetic variation ; biplot ; drought stress ; drying processes ; mathematical model ; plant hydric stress tolerance ; rate of weight loss ; RWLMod ; water evaporation ; photosynthesis ; elevated CO2 ; Rubisco ; electron transport ; light ; diurnal cycle ; sexual propagation ; cold stratification ; in situ ; ex situ ; plant endemism ; Morocco ; biodiversity ; ex-situ conservation ; protocols ; germplasm ; forest berries ; brushing ; lettuce ; chicory ; phytochemicals ; antioxidant capacity ; Ziziphus lotus ; phenolics ; SH-SY5Y cell line ; chromatography ; Koelreuteria paniculata ; dry ethanol extracts ; GC-MS analysis ; chemical compounds ; antitumor and antimicrobial activities ; medicinal plant ; bioactive compounds ; plant-derived secondary metabolites (PDSM) ; cell suspension culture (CSC) ; bioreactor engineering ; apple ; Golden Delicious ; Top Red ; fruitlet thinners ; light reactions ; electron transport rate ; photoprotective mechanism ; state transitions ; PSII repair cycle ; vegetation structure ; environmental variables ; PC-ORD ; plant community assembly ; Himalaya ; allopolyploidy ; interspecific hybridization ; unreduced gametes ; cytological diploidization ; genomic changes ; root length ; root/shoot ratio ; specific root length ; Saragolle Lucana ; seed coating ; heavy metals ; evolution ; hyperaccumulation ; black chokeberry (Aronia melanocarpa) ; anthocyanin stability ; herbs ; co-pigmentation ; color stability ; functional foods/beverages ; biotechnological tools ; ethnomedicine ; in vitro culture ; genetic improvement ; pollen ; tip growth ; calcium ; calcium dependent protein kinase ; Rho Guanine Dissociation Inhibitor ; ROP GTPase ; RhoGDI displacement factor ; polarity ; guar ; gene expression ; qRT-PCR ; RNA-Seq ; salt stress ; salt tolerance ; stress ; transcriptome ; D-tagatose ; IFP48 ; induced resistance ; sweet immunity ; sugar-enhanced defense ; Plasmopara viticola ; Botrytis cinerea ; Vitis vinifera ; human diet ; edible wild plants ; Plantago coronopus L. ; Rumex acetosa L. ; Cichorium intybus L. ; Artemisia dracunculus L. ; phytochemistry ; anti-inflammatory properties ; stem photosynthesis ; hydraulic recovery ; soaking ; X-ray micro-CT ; bark water uptake ; embolism ; genetic resources ; Solanaceae ; Cucumis ; Lactuca ; diversity ; vegetables ; genebank ; essential oil ; iNOS ; interleukin ; lavenders ; NF-κB ; glycosyltransferases ; ER-Golgi trafficking ; mechanism of protein sorting ; COPI and COPII complexes ; sequences and motifs involved in trafficking ; Arabidopsis ; gene regulation ; protein-protein interaction ; transcription factor ; WRI1 ; TCP20 ; lipases ; lipid metabolism ; plant-environment interactions ; reproductive development ; vegetative development ; Urtica dioica ; soilless systems ; cultivated nettle ; stress factors ; functional properties ; preharvest sprouting ; MKK3 ; maternal and paternal expressed genes ; imprinted genes ; polycomb repressive complex 2 ; mRNA processing bodies ; ribonucleic binding proteins ; monosomes ; ethylene ; elicitors ; fruit ripening ; ACC synthase/oxidase ; GC-MS ; polyamines ; Vigna genus ; introgression ; hybridisation ; phylogeny ; de novo domestication ; feralisation ; novel ecosystems ; complex networks ; tree communities ; Lantana camara ; Prosopis juliflora ; ascorbic acid ; genetic diversity ; molecular markers ; aquaculture pond sediment ; recovery ; Triticum aestivum ; chlorophyll fluorescence ; wheat grass juice quality ; UV-B radiation ; olive tree ; metabolomic ; phenolic profile ; lipophilic profile ; ecophysiology ; environment ; arbuscular mycorrhizal symbiosis ; comparative transcriptomics ; Arum-type ; Paris-type ; Solanum lycopersicum ; Rhizophagus irregularis ; Gigaspora margarita ; Camelina sativa ; semi-arid lands ; biofuel feedstock ; biodiesel ; renewable diesel ; crop breeding ; transgenesis ; genome editing ; Xanthomonas euvesicatoria ; host associate factor ; comparative genomics ; Cannabis sativa L. ; chemovars ; secondary metabolites ; trichomes ; residual by-products ; biogeography ; cardioid ; islands ; geometric models ; Mediterranean flora ; Silene ; super-ellipse ; abscisic acid ; aromatic herb ; ascorbate-glutathione cycle ; jasmonic acid ; lipoic acid ; oxidative stress ; salicylic acid ; Salvia officinalis ; drought ; state of stress ; tolerance ; avoidance ; stress survival ; amino acids ; nitrate reductase ; glutamine synthetase ; plants mycorrhized ; dark septate ; Daphne genkwa ; Thymelaeaceae ; flavonoids ; design of experiments ; blooming stages ; germination stimulant ; witchweed ; methyl phenlactonoates (MPs) ; Nijmegen-1 ; weed ; plant development ; vasculature ; leaf traces ; structure ; microtomography ; Euphorbiaceae ; in vitro crop ; gamma radiation ; ionizing radiation ; mutants ; Fumaria scheleicheri Soy. Will. ; isoquinoline alkaloids ; HPLC-DAD ; in vitro anti-cholinesterase ; cytotoxic ; antioxidant ; ABC model ; hop ; transcription factors ; type-II MADS box ; type-I MADS-box ; AFLP ; carpological traits ; genetic structure ; molecular systematics ; plastid phylogeny ; Valerianaceae ; auxins ; embryogenic calli ; HPLC ; IAA ; immunohistochemistry ; deficit irrigation ; grape quality ; phenology ; plant diseases ; bacterium ; symptoms ; molecular classification ; common juniper ; common larch ; Cupressaceae ; Pinaceae ; SPME-GC-MS ; volatile compounds ; herbicidal activity ; weed control ; trait association ; GCV ; genetic variability ; genetic advance ; heritability ; PCV ; Cicer arietinum L. ; gold nanoparticles ; carbon nanotubes ; ATR-FTIR spectroscopy ; machine learning techniques ; principal component analysis ; support vector machine classification ; citrus ; melanose ; Diaporthe citri ; epidemiology ; symptomatology ; Pseudomonas cannabina pv. alisalensis ; resistance-nodulation-cell division transporter ; type-three secretion system ; phytoalexin ; brassinin ; glucosinolate ; cabbage ; flowering ; juvenile traits ; genetic stability ; flow cytometry ; somaclonal variation ; thorniness ; carbohydrates ; protein ; lipids ; fatty acids ; minerals ; plastome ; Plicosepalus acaciae ; Plicosepalus curviflorus ; loranthaceae ; mistletoe ; phylogenetic relationship ; plastome structure ; comparative analysis ; magnetic resonance imaging ; Solanum tuberosum ; multi-exponential transverse relaxation ; water stress ; broccoli ; human nutrition ; improved health ; melatonin ; postharvest ; apricot ; pollen tube ; pollination ; Prunus armeniaca ; S-alleles ; Populus ; hexokinase ; sucrose metabolism ; sugar signaling ; stress and defense ; centres of origin ; crop wild relatives ; crop domestication ; cryopreservation ; conservation ; in vitro storage ; ecosystem restoration ; plant breeding ; acidification ; alkalinisation ; bud burst ; freezing ; Malus domestica ; pH ; Picea abies ; Pinus cembra ; histone modification ; Taraxacum kok-saghyz ; natural rubber ; high light stress ; singlet oxygen ; signalling ; GPX5 ; beta cyclocitral ; acrolein ; glutathione peroxidase ; carbonyl ; transcription ; SLIM1 transcription factor ; sulfur deficiency ; Arabidopsis thaliana ; sulfate transporter ; sulfate assimilation ; stress tolerance ; LRR-RLK receptors ; dodders ; parasitic plants ; proteomics ; virus vertical transmission ; CMV-Fny strain ; pseudorecombinant virus ; chimeric virus ; infection rate ; seed-growth tests ; electron microscopy ; circular dichroism spectroscopy ; viral assembly ; Adiantetea capilli-veneris ; demographic analysis ; ecology ; IUCN ; plant conservation ; phytosociology ; rupicolous habitat ; n/a ; bic Book Industry Communication::G Reference, information & interdisciplinary subjects::GP Research & information: general ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences
    Language: English
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  • 7
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2022-10-25
    Description: Over the last few years, the subject of food authenticity and food fraud has received increasing attention from consumers and other stakeholders, such as government agencies and policymakers, control labs, producers, industry, and the research community. Among the different approaches aiming to identify, tackle, and/or deter fraudulent practices in the agri-food sector, the development of new, fast, and accurate methodologies to evaluate food authenticity is of major importance. This book, entitled “Target and Non-Target Approaches for Food Authenticity and Traceability”, gathers original research and review papers focusing on the development and application of both targeted and non-targeted methodologies applied to verify food authenticity and traceability. The contributions regard different foods, among which some are frequently considered as the most prone to adulteration, such as olive oil, honey, meat, and fish. This book is intended for readers aiming to enrich their knowledge through reading contemporary and multidisciplinary papers on the topic of food authentication.
    Keywords: COIBar–RFLP (cytochrome oxidase I barcode–restriction fragment length polymorphism) ; seafood ; fraud ; DNA barcoding ; food authenticity ; food adulteration ; food fraud ; donkey ; cytochrome b ; real-time PCR ; meat products ; honey ; regional origin ; chemometric analysis ; mineral content ; Montenegro ; Sepia ; common cuttlefish ; Sepia officinalis ; real-time PCR (Polymerase Chain Reaction) ; species identification ; food authentication ; COI (Cytochrome Oxidase I) ; Olea europaea var Sylvestris ; oleaster ; olive ; olive oil ; adulteration ; SNP ; DNA ; virgin olive oil ; quality ; volatile compounds ; sensory analysis ; chemometrics ; anti food fraud ; Curcuma longa ; DNA markers ; SYBR-GREEN real-time PCR ; Zea mays ; pasta ; Triticum aestivum ; Triticum durum ; genetic traceability ; digital PCR ; semolina ; species ; truffle ; Tuber spp. ; species differentiation ; near-infrared spectroscopy ; red deer ; roe deer ; water deer ; multiplex PCR ; capillary electrophoresis ; perilla ; sesame ; geographic origin ; metabolomics ; multivariate analysis ; metabolite profiling ; quantification ; chicken ; guinea fowl ; pheasant ; quail ; turkey ; authentication ; authenticity ; chemometric ; fish ; origin ; meat ; milk ; spectroscopy ; 1H-NMR ; GC-MS ; HPLC-UV/VIS ; protein hydrolysate ; free amino acid contents ; ProHydrAdd ; monofloral honey ; direct analysis in real time (DART) ; high resolution mass spectrometry (HRMS) ; geographical origin ; Ginkgo biloba ; plant infusions ; real-time polymerase chain reaction ; DNA extraction ; opium poppy ; seed ; pollen grains ; bakery product ; oil ; PCR ; Salmo salar L. ; fatty acids ; mislabeling ; machine learning ; n/a ; bic Book Industry Communication::T Technology, engineering, agriculture::TB Technology: general issues
    Language: English
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  • 8
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2024-04-11
    Description: This book focuses on the fundamental and applied research of the non-destructive estimation and diagnosis of crop leaf and plant nitrogen status and in-season nitrogen management strategies based on leaf sensors, proximal canopy sensors, unmanned aerial vehicle remote sensing, manned aerial remote sensing and satellite remote sensing technologies. Statistical and machine learning methods are used to predict plant-nitrogen-related parameters with sensor data or sensor data together with soil, landscape, weather and/or management information. Different sensing technologies or different modelling approaches are compared and evaluated. Strategies are developed to use crop sensing data for in-season nitrogen recommendations to improve nitrogen use efficiency and protect the environment.
    Keywords: UAS ; multiple sensors ; vegetation index ; leaf nitrogen accumulation ; plant nitrogen accumulation ; pasture quality ; airborne hyperspectral imaging ; random forest regression ; sun-induced chlorophyll fluorescence (SIF) ; SIF yield indices ; upward ; downward ; leaf nitrogen concentration (LNC) ; wheat (Triticum aestivum L.) ; laser-induced fluorescence ; leaf nitrogen concentration ; back-propagation neural network ; principal component analysis ; fluorescence characteristics ; canopy nitrogen density ; radiative transfer model ; hyperspectral ; winter wheat ; flooded rice ; pig slurry ; aerial remote sensing ; vegetation indices ; N recommendation approach ; Mediterranean conditions ; nitrogen ; vertical distribution ; plant geometry ; remote sensing ; maize ; UAV ; multispectral imagery ; LNC ; non-parametric regression ; red-edge ; NDRE ; dynamic change model ; sigmoid curve ; grain yield prediction ; leaf chlorophyll content ; red-edge reflectance ; spectral index ; precision N fertilization ; chlorophyll meter ; NDVI ; NNI ; canopy reflectance sensing ; N mineralization ; farmyard manures ; Triticum aestivum ; discrete wavelet transform ; partial least squares ; hyper-spectra ; rice ; nitrogen management ; reflectance index ; multiple variable linear regression ; Lasso model ; Multiplex®3 sensor ; nitrogen balance index ; nitrogen nutrition index ; nitrogen status diagnosis ; precision nitrogen management ; terrestrial laser scanning ; spectrometer ; plant height ; biomass ; nitrogen concentration ; precision agriculture ; unmanned aerial vehicle (UAV) ; digital camera ; leaf chlorophyll concentration ; portable chlorophyll meter ; crop ; PROSPECT-D ; sensitivity analysis ; UAV multispectral imagery ; spectral vegetation indices ; machine learning ; plant nutrition ; canopy spectrum ; non-destructive nitrogen status diagnosis ; drone ; multispectral camera ; SPAD ; smartphone photography ; fixed-wing UAV remote sensing ; random forest ; canopy reflectance ; crop N status ; Capsicum annuum ; proximal optical sensors ; Dualex sensor ; leaf position ; proximal sensing ; cross-validation ; feature selection ; hyperparameter tuning ; image processing ; image segmentation ; nitrogen fertilizer recommendation ; supervised regression ; RapidSCAN sensor ; nitrogen recommendation algorithm ; in-season nitrogen management ; nitrogen use efficiency ; yield potential ; yield responsiveness ; standard normal variate (SNV) ; continuous wavelet transform (CWT) ; wavelet features optimization ; competitive adaptive reweighted sampling (CARS) ; partial least square (PLS) ; grapevine ; hyperparameter optimization ; multispectral imaging ; precision viticulture ; RGB ; multispectral ; coverage adjusted spectral index ; vegetation coverage ; random frog algorithm ; active canopy sensing ; integrated sensing system ; discrete NIR spectral band data ; soil total nitrogen concentration ; moisture absorption correction index ; particle size correction index ; coupled elimination ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TB Technology: general issues::TBX History of engineering and technology ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TQ Environmental science, engineering and technology
    Language: English
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  • 9
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    KIT Scientific Publishing
    Publication Date: 2024-04-05
    Description: Die Arbeit stellt die Hauptlinien der im 20. Jahrhundert in West- und Osteuropa geführten Totalitarismus-Debatten dar, um die besonderen Merkmale von Cassirers Auseinandersetzung mit demselben Phänomen des Totalitarismus zu zeigen. Der Autor konzentriert sich auf Cassirers Kulturphilosophie und politischer Philosophie im Verhältnis zu den dargestellten Debatten. So wird geklärt, welchen Gewinn Cassirers kulturanthropologisch angelegte Deutung totalitärer Herrschaft aus heutiger Sicht abwirft.
    Keywords: Ernst Cassirer ; Mythos ; Symbol ; Staat ; Politische Philosophie ; Ideologie ; Nationalsozialismus ; Kommunismus ; Totalitarismus ; Pathologie ; Rat ; thema EDItEUR::Q Philosophy and Religion::QD Philosophy
    Language: German
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  • 10
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2024-04-05
    Description: Allohexaploid bread wheat and diploid barley are two of the most cultivated crops in the world. This book reports novel research and reviews concerning the use of modern technologies to understand the molecular bases for wheat and barley improvement. The contributions published in this book illustrate research advances in wheat and barley knowledge using modern molecular techniques. These molecular approaches cover genomic, transcriptomic, proteomic, and phenomic levels, together with new tools for gene identification and the development of novel molecular markers. Overall, the contributions for this book lead to a further understanding of regulatory systems in order to improve wheat and barley performance.
    Keywords: QH301-705.5 ; Q1-390 ; n/a ; biotechnology ; transgene ; Aegilops tauschii ; antioxidant enzymes ; aquaporin ; molecular marker ; Kompetitive Allele Specific PCR (KASP) ; transgenic wheat ; purple acid phosphatase phytase ; genome editing ; genes ; resistance ; genome assembly ; germination ; protein two-dimensional electrophoresis ; 1 ; disease resistance ; Thinopyrum ; plant ; oligo probe ; optical mapping ; genetic biofortification ; breeding ; population structure ; marker-assisted selection ; crops ; hybrid necrosis ; PAPhy ; Triticeae ; wheat ; Barley ; genome stability ; CRISPR ; powdery mildew ; RNA editing ; bread wheat ; allohexaploid ; nucleus ; chromatin ; introgression ; favorable alleles ; genetic engineering ; Tunisian landraces ; barely ; Pm40 ; Blumeria graminis f. sp. tritici ; Transcriptional dynamics ; Lr42 ; Triticum durum ; histochemical analysis ; molecular mapping ; ribosomal DNA ; 12-oxophytodienoate reductase ; small segment translocation ; HIGS ; Powdery mildew ; abiotic stress ; phytase ; RNA-seq ; Bulked segregant analysis-RNA-Seq (BSR-Seq) ; grain ; DArTseq technology ; center of diversity ; mature grain phytase activity (MGPA) ; cereals ; Grain development ; hybrid ; homoeolog ; 3D-FISH ; jasmonates ; Single nucleotide polymorphism (SNP) ; genetic diversity ; ND-FISH ; durum wheat ; protease ; transpiration ; TdPIP2 ; cereal cyst nematodes ; mass spectrometry ; 6R ; Landrace ; marker-trait associations ; BAC ; chromosome ; barley ; freezing tolerance ; KASP markers ; Triticum aestivum ; rye ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
    Language: English
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  • 11
    Publication Date: 2024-01-12
    Description: The Mycosphaerella complex is both poly- and paraphyletic, containing several different families and genera. The genus Mycosphaerella is restricted to species with Ramularia anamorphs, while Septoria is restricted to taxa that cluster with the type species of Septoria, S. cytisi, being closely related to Cercospora in the Mycosphaerellaceae.\nSpecies that occur on graminicolous hosts represent an as yet undescribed genus, for which the name Zymoseptoria is proposed. Based on the 28S nrDNA phylogeny derived in this study, Zymoseptoria is shown to cluster apart from Septoria. Morphologically species of Zymoseptoria can also be distinguished by their yeast-like growth in culture, and the formation of different conidial types that are absent in Septoria s.str. Other than the wellknown pathogens such as Z. tritici, the causal agent of septoria tritici blotch on wheat, and Z. passerinii, the causal agent of septoria speckled leaf blotch of barley, both for which epitypes are designated, two leaf blotch pathogens are also described on graminicolous hosts from Iran. Zymoseptoria brevis sp. nov. is described from Phalaris minor, and Z. halophila comb. nov. from leaves of Hordeum glaucum. Further collections are now required to elucidate the relative importance, host range and distribution of these species.
    Keywords: Hordeum vulgare ; ITS ; LSU ; multilocus sequence typing ; Mycosphaerella ; Septoria ; systematics ; Triticum aestivum
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 59-68 
    ISSN: 1573-0867
    Keywords: bicarbonate-extractable potassium ; muriate of potash ; potassium ; potassium chloride ; relative effectiveness ; silicate rock powder ; Triticum aestivum ; Trifolium subterraneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Granite (silicate) rock dust, a by-product of quarry operations, is being advocated and used as a fertilizer in the wheatbelt of south-western Australia (WA). The dust is insoluble and based on its nutrient element content (1.9% K and 0.3%P and negligible N) it is not expected to be a useful fertilizer. Previous laboratory studies and glasshouse experiments in WA suggest the dust is a slow release K fertilizer. This paper extends the previous studies to consider the dust as an NP or K fertilizer in the year of application in a field experiment on a soil deficient in N, P and K. In addition, the effectiveness of the dust as a K fertilizer was compared with the effectiveness of KCl (muriate of potash), the K fertilizer used in WA at present, in glasshouse experiments using K deficient soils. In the field experiment, compared with NP fertilizer or NPK fertilizer (urea, supplying N; superphosphate, providing P, S, Ca, Cu, Zn and Mo; KCl providing K), the dust had no effect on grain yield of wheat (Triticum aestivum); in fact dust applied at 20 t ha-1, for unknown reasons, reduced yields by about 65% compared to the nil (no fertilizer, no dust) treatment. Relative to the nil treatment, applying NPK fertilizer increased yields about threefold, from 0.54 to 1.79 t ha. The glasshouse experiments showed that, relative to KCl, the dust was from about 0.02 to 14% as effective in K deficient grey sandy soils for producing dried tops of 30-day old wheat plants or 42-day old clover (Trifolium subterraneum) plants. In soils with adequate K (yellow sands, sandy loams or clays, loamy clays, clay loams and clays), neither KCl nor the dust affected yields of 30 to 42-day old wheat or clover plants grown in the glasshouse. In the glasshouse experiments, no yield depressions were measured for the dust applied up to 17 g dust per kg soil (equivalent to 17 t dust ha-1 mixed into the top 10 cm of soil in the field). It is concluded that the dust has no value as a fertilizer.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 56 (2000), S. 117-123 
    ISSN: 1573-0867
    Keywords: labelled nitrogen ; Lolium perenne ; nitrogen cycling ; root biomass ; straw ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Large amounts of nitrogen (N) fertiliser (150–200 kg N/ha) are currently being applied to perennial ryegrass (Lolium perenneL.) seed crops in New Zealand. Due to increasing requirements for efficient use of N fertilisers and minimising nitrate contamination of the environment, a field experiment was established using 15N-labelled fertiliser to follow the fate of applied N. Urea-15N was applied to a perennial ryegrass seed crop in April (30 kg N/ha), August (30 kg N/ha), September (60 kg N/ha) and October (60 kg N/ha). The urea-15N was applied in solution and watered in to minimise volatilisation loss. At the time of harvest (December), 9% of the applied 15N was in the seed, 29% in the straw, 19% in the roots and 39% in the soil organic matter. Losses of 15N were minimal as the N was applied in several applications, each one at a relatively low rate, and at times when leaching was unlikely to occur. Ryegrass plants used a greater proportion of the N applied in September and October (61–65%) compared with that applied in April (44%). Consequently more N was recovered from the soil in the autumn application (57%) than from the September and October applications (28–44%). The availability of the residual fertiliser N to a subsequent wheat (Triticum aestivum L.) crop was studied in a glasshouse experiment. The residual fertiliser N was present in the soil and ryegrass roots and stubble. The wheat plants only recovered 7–9% of this residual N. Most of the N taken up by the wheat came from the soil organic N pool. Overall, applying a total of 180 kg N/ha to the ryegrass appeared to have minimal direct impact on the environment. In the short term N not used by the ryegrass plants contributed to the soil organic N pool.
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  • 14
    ISSN: 1432-1211
    Keywords: Key words Vβ13 ; CD4/CD8 ratio ; Rat ; Tcrb ; Polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Three rat BV13S1 alleles (T-cell receptor β-chain variable gene 13) were characterized by new BV13S1-allele specific monoclonal antibodies (18B1 and 17D5) and sequence analysis of expressed and genomic BV13S1. Two alleles were functional and designated BV13S1A1 present in strains LEW, BUF, PVG, and BV13S1A2 present in BN and WF. Their products differed by six amino acids, two of them in complementarity-determing region (CDR)1 and one in CDR2. A third nonfunctional allele, BV13S1A3P, was found in strains F344 and DA. Apart from a single nucleotide insertion, it was identical to BV13S1A2. All 12 rat strains tested showed association of TCRBC1 with BV8S2/4 alleles but not with the BV13S1 alleles, which may reflect a different gene order of the rat BV compared to mouse. BV13S1A1-encoded T-cell receptors (TCRs) which bind both monoclonal antibody (mAb) 18B1 and mAb 17D5 are over-represented in the CD4 lymphocyte subset. BV13S1A2-encoded TCRs which are stained by mAb 18B1 but not by mAb 17D5 show a slight CD8-biased expression. Preferential usage of BV13S1A1-positive TCRs by CD4 but not by CD8 cells in (LEW×WF)F1 hybrids and cosegregation of BV13SA1 and increased frequency of BV13S1 TCR-positive CD4 cells in a (LEW×BN)×BN backcross suggest structural differences of the two allelic products as the reason for their contrasting CD4/CD8 subset bias.
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  • 15
    ISSN: 1432-119X
    Keywords: Endothelin-A receptor ; Endothelin-B receptor ; Rat ; Pulmonary fibrosis ; Immunohistochemistry ; Quantitative PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: AbstractPulmonary fibrosis is characterized by excessive extracellular matrix deposition with concomitant loss of gas exchange units, and endothelin-1 (ET-1) has been implicated in its pathogenesis. Increased levels of ET-1 from tissues and bronchoalveolar lavage have been reported in patients with pulmonary fibrosis and in animal models after intratracheal bleomycin. We characterized the cellular distribution of alveolar ET receptors by immunohistochemistry in bleomycin-induced pulmonary fibrosis in the rat and determined the regulation by bleomycin of ET receptor mRNA expression in isolated alveolar macrophages and rat lung fibroblasts. We found significant increases in the numbers of fibroblasts and macrophages at day 7 compared to day 28 and control animals. ETB receptor immunoreactivity was observed on fibroblasts and invading monocytes. Isolated fibroblasts expressed both ETA and ETB receptor mRNA, and ETA receptor mRNA was upregulated by bleomycin. Isolated resident alveolar macrophages expressed neither ETA nor ETB receptor mRNA which were also not induced by bleomycin. We conclude that, while ETB receptor stimulation of fibroblasts and monocytes recruited during bleomycin-induced lung injury exerts antagonistic effects on fibroblast collagen synthesis, the observed increase in the number of fibroblasts in vivo and upregulation of fibroblast ETA receptor mRNA by bleomycin in vitro point to a predominance of the profibrotic effects of ET receptor engagement.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Sciences of soils 5 (2000), S. 10-21 
    ISSN: 1432-9492
    Keywords: Soil temperature ; Triticum aestivum ; Stubble retention ; Nitrogen ; Early growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Early growth and development are often lower when wheat is sown into standing stubble. A study was conducted to determine whether this difference in early growth could be explained by the effects of stubble on soil temperature in the vicinity of the young plant. The roles of nitrogen nutrition and soil strength were also assessed. Three crops were monitored (1990–1992), with the wheat being sown into either standing wheat stubble after a no-till fallow (NT), or into no-tilled plots from which the stubble had been removed by burning (NB). Measurements were made of wheat growth and development, soil and plant N, soil temperature and penetration resistance. The site was on a black earth near Warialda in the northern wheatbelt of New South Wales, Australia. In 1992 wheat was also grown under simulated stubble to isolate the shading and soil temperature effects of stubble from other factors. A significant (P〈0.05) relationship was found between average soil temperature and above ground dry matter (DM) at 65 days after sowing (DAS) but not at 107 DAS. This relationship accounted for differences in DM production at 65 DAS between NT and NB treatments in 1991 and 1992, but not in 1990. In that year the lower DM production in NT plots was associated with poorer N nutrition, and possibly disease. Laboratory incubations indicate that immobilisation of N as stubble decomposed could have contributed to this. Burning stubble produced no immediate increase in soil N availability, so that it is unlikely that N contained in stubble contributed to the difference. Soil strength differences between treatments and phytotoxic effects are unlikely to have contributed to growth differences in this soil.
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  • 17
    ISSN: 1432-2242
    Keywords: Key words Glutenin ; Triticum aestivum ; Wheat storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Low-molecular-weight (LMW) glutenin subunits consist mainly of two domains, one at the N- terminus which contains repeats of short amino-acid motifs, and a non-repetitive one rich in cysteine, at the C- terminal region. In previous reports, polyacrylamide-gel electrophoresis has been used to show that large size variation exists among LMW and HMW glutenin subunits, and it has been suggested that deletions and insertions within the repetitive region are responsible for these variations in length. In this study, PCR-amplification of genomic DNA (Triticum aestivum variety Chinese Spring) was used to isolate three full-length LMW glutenin genes: LMWG-MB1, LMWG-MB2 and LMWG-MB3. The deduced amino-acid sequences show a high similarity between these ORFs, and with those of other LMW glutenin genes. Comparisons indicate that LMWG-MB1 has probably lost a 12-bp fragment through deletion and that LMWG-MB1 and LMWG-MB2 have an insertion of 81 bp within the repetitive domain. The current study has shown direct evidence that insertions and/or deletions provide a mechanistic explanation for the allelic variation, and the resultant evolution, of prolamin genes. Single-base substitutions at identical sites generate stop codons in both LMWG-MB2 and LMWG-MB3 indicating that these clones are pseudogenes.
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  • 18
    ISSN: 1432-2242
    Keywords: Key words Wheat ; High-molecular-weight glutenin ; AS-PCR ; Glu-A1 locus ; Null allele ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The present work reports new PCR markers that amplify the complete coding sequence of the specific alleles of the high molecular weight (HMW) glutenin genes. A set of AS-PCR molecular markers was designed which use primers from nucleotide sequences of the Glu-A1 and Glu-D1 genes, making use of the minor diffeences between the sequences of the x1, x2* of Glu-A1, and the x5 and y10 of Glu-D1. These primers were able to distinguish between x2* and the x1 or xNull of Glu-A1. Also x5 was distinguishable from x2, and y10 from y12. The primers amplified the complete coding regions and corresponded to the upstream and downstream flanking positions of Glu-A1 and Glu-D1. Primers designed to amplify the Glu-A1 gene amplified a single product when used with genomic DNA of common wheats and the xNull allele of this gene. This work also describes the cloning and characterisation of the nucleotide sequence of this allele. It possesses the same general structure as x2* and x1 (previously determined) and differs from these alleles in the extension of the coding sequence for a presumptive mature protein with only 384 residues. This is due to the presence of a stop codon (TAA) 1215-bp downstream from the start codon. A further stop codon (TAG), 2280-bp downstream from the starting codon is also found. The open reading frame of xNull and x1 alleles has the same size in bp. Both are larger than x2* which shows two small deletions. The reduced size of the presumptive mature protein encoded by xNull could explain the negative effect of this allele on grain quality.
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  • 19
    ISSN: 1432-2242
    Keywords: Keywords AFLPs ; Bulked segregant analysis ; Marker-assisted selection ; Microsatellites ; Powdery mildew resistance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Molecular markers were identified in common wheat for the Pm24 locus conferring resistance to different isolates of the powdery mildew pathogen, Erysiphe graminis DM f. sp. tritici (Em. Marchal). Bulked segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers and microsatellite markers linked to the gene Pm24 in an F2 progeny from the cross Chinese Spring (susceptible)× Chiyacao (resistant). Two AFLP markers XACA/CTA-407 and XACA/CCG-420, and three microsatellite markers Xgwm106, Xgwm337 and Xgwm458, were mapped in coupling phase to the Pm24 locus. The AFLP marker locus XACA/CTA-407 co-segregated with the Pm24 gene, and XACA/CCG-420 mapped 4.5 cM from this gene. Another AFLP marker locus XAAT/CCA-346 co- segregated in repulsion phase with the Pm24 locus. Pm24 was mapped close to the centromere on the short arm of chromosome 1D, contrary to the previously reported location on chromosome 6D. Pm24 segregated independently of gene Pm22, also located on chromosome 1D. An allele of microsatellite locus Xgwm337 located 2.4±1.2 cM from Pm24 was shown to be diagnostic and therefore potentially useful for pyramiding two or more genes for powdery mildew resistance in a single genotype.
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  • 20
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    Theoretical and applied genetics 100 (2000), S. 32-38 
    ISSN: 1432-2242
    Keywords: Key words Waxy (Wx) protein ; Triticum aestivum ; Amylose content ; Starch ; Rapid Visco-Analyzer ; Swelling power ; Noodle quality
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    Topics: Biology
    Notes: Abstract  Waxy (Wx) protein is a granule-bound starch synthase (GBSS) responsible for amylose production in cereal endosperm. Eight isolines of wheat (Triticum aestivum L.) having different combinations of presence and absence of three Wx proteins, Wx-A1, -B1, and -D1, were produced in order to elucidate the effect of Wx protein deficiencies on the apparent amylose content and starch-pasting properties. An improved SDS gel electrophoresis showed that ’Bai Huo’ (a parental wheat) carried a variant Wx-B1 protein from an allele, Wx-B1e. Thus, wheat lines of types 1, 2, 4, and 6 examined in this study contained a variant Wx-B1 allele and not the standard allele, Wx-B1a. The results from 3 years of experiments using 176 lines derived from two cross-combinations showed that apparent amylose content increased the least in type 8 (waxy) having no Wx proteins and, in ascending order, increased in type 5 (only the Wx-A1 protein is present) 〈type 7 (Wx-D1) 〈type 6 (Wx-B1) 〈type 3 (Wx-A1 and -D1) 〈type 4 (Wx-A1 and -B1) 〈type 2 (Wx-B1 and -D1) 〈type 1 (three Wx proteins). However, Tukey’ s studentized range test did not detect significant differences in some cases. Densitometric analysis suggested that the amylose content was related to the amount of the Wx protein in the eight types. Parameters in the Rapid Visco-Analyzer test and swelling power were correlated to amylose content. Consequently, amylose content and pasting properties of starch were determined to be influenced the most by the lack of the Wx-B1 protein, followed by a lack of Wx-D1, and leastly by the Wx-A1 deficiency, which indicated the presence of differential effects of the three null alleles for the Wx protein.
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  • 21
    ISSN: 1432-2242
    Keywords: Keywords Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Residual resistance effects ; Quantitative resistance
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract  The evolution of adult plant resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) was investigated in 11 wheat populations cultivated for 10 years in a French network for dynamic management (DM) of wheat genetic resources. The aims of the study were to compare the evolution of resistance in sites submitted to different powdery mildew pressure and to investigate the implication of specific resistance gene action in adult plant resistance. For this, 7 of the 11 populations were characterized for their composition of specific resistance genes (results presented in a former paper). Even though no population differed significantly from the initial PA0 pool for mean adult plant resistance, divergence appeared among the final populations. The populations with the highest adult plant resistance level originated from sites where powdery mildew pressure is known to be high (Vervins, Le Rheu), whereas populations with the lowest adult plant resistance corresponded to areas with no, or very low, powdery mildew pressure (Toulouse, Montreuil-Bellay). A residual effect of defeated specific resistance genes was hypothesized, as lines accumulating at least two specific resistance genes appeared more resistant. Additional quantitative resistance seemed to be involved in adult plant resistance. DM lines appeared then as an interesting source of variability for resistance towards powdery mildew. Moreover, as these lines had been grown in mixed populations they may be appropriate as components of a composite cultivar.
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  • 22
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    Theoretical and applied genetics 100 (2000), S. 519-527 
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Triticum aestivum ; Physical mapping ; Deletion lines ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Extended physical maps of chromosomes 6A, 6B and 6D of common wheat (Triticum aestivum L. em Thell., 2n=6x=42, AABBDD) were constructed with 107 DNA clones and 45 homoeologous group-6 deletion lines. Two-hundred and ten RFLP loci were mapped, including three orthologous loci with each of 34 clones, two orthologous loci with each of 31 clones, one locus with 40 clones, two paralogous loci with one clone, and four loci, including three orthologs and one paralog, with one clone. Fifty five, 74 and 81 loci were mapped in 6A, 6B and 6D, respectively. The linear orders of the mapped orthologous loci in 6A, 6B and 6D appear to be identical and 65 loci were placed on a group-6 consensus physical map. Comparison of the consensus physical map with eight linkage maps of homoeologous group-6 chromosomes from six Triticeaespecies disclosed that the linear orders of the loci on the maps are largely, if not entirely, conserved. The relative distributions of loci on the physical and linkage maps differ markedly, however. On most of the linkage maps, the loci are either distributed relatively evenly or clustered around the centromere. In contrast, approximately 90% of the loci on the three physical maps are located either in the distal one-half or the distal two-thirds of the six chromosome arms and most of the loci are clustered in two or three segments in each chromosome.
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  • 23
    ISSN: 1432-2242
    Keywords: Key words Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Selection ; Drift
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract  Dynamic management has been proposed as a complementary strategy to gene banks for the conservation of genetic resources. The evolution of frequencies of genes for specific resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) in populations of a French network for dynamic management of bread wheat genetic resources was investigated after 10 years of multiplication without human selection. The objective was to determine whether specific resistance gene diversity was maintained in the populations and whether any changes could be attributed to selection due to pathogen pressure. Seven populations, originating from four of the network sites, were characterized and compared to the initial population for six specific resistance gene frequencies detected by nine Blumeria graminis f. sp. tritici isolates. Diversity decreased at the population level, but because of a strong differentiation between the populations, this diversity was maintained at the network level. The comparison of Fst parameters estimated on neutral markers (RFLP) and on resistance gene data revealed that in two of the populations specific resistance genes had been selected by pathogen pressure, whereas evolution in two other populations seemed to be the result of genetic drift. For the three last populations, conclusions were less clear, as one had probably experienced a strong bottleneck and the other two presented intermediate Fst values. A dynamic management network with sites contrasted for pathogen pressure, allowing genetic drift in some populations and selection in others, appeared, at least on the short term, to be a good tool for maintaining the diversity of genes for specific resistance to powdery mildew.
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  • 24
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    Russian journal of plant physiology 47 (2000), S. 734-739 
    ISSN: 1608-3407
    Keywords: Triticum aestivum ; bioelectric potentials ; frost resistance ; critical points
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Winter wheat (Triticum aestivum L.) seedlings of three cultivars differing in frost resistance were used to study cooling-induced changes in the bioelectric potential. Measurements were performed with nonfreezing graphite–glycerol electrodes in the regime of monitoring. Upon a gradual change in air temperature from 20 to –15°C at the rates of 20 and 2°C/h, the bioelectric potential underwent abrupt transitions at certain moments, indicating changes in the physiological condition of plants. The time required for the achievement of these critical states, as well as the survival of plants after thawing, depended both on the temperature and the cooling rate. Apparently, these characteristics were related to the dynamics of phase transitions of water. Cultivar-specific features were manifested in the different abilities of plants to maintain free water in a supercooled state. It is supposed that the critical points are related to the cold resistance of colloid systems and to the temperature lethal for plants.
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  • 25
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    Annals of biomedical engineering 28 (2000), S. 128-134 
    ISSN: 1573-9686
    Keywords: Hippocampus ; Vigilance states ; Paired-pulse ; Dentate gyrus ; Dentate granule cells ; Evoked response ; Rat ; In vivo studies ; Perforant path ; Maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract This study examined the effect of normal development and vigilance state on the modulation of dentate granule cell activity in the freely moving rat at 15, 30, and 90 days of age across three vigilance states: quiet waking, slow-wave sleep, and rapid eye movement sleep. Using paired-pulse stimulation, the paired-pulse index (PPI) was obtained for the dentate evoked field potentials elicited by the stimulation of the medial perforant path. Although significant differences in PPI values were observed during development, no significant vigilance state related changes were obtained. Preweaning infant rats, i.e., 15-day old, exhibited significantly less early (interpulse intervals, IPI= 20–50 ms) and late (IPI = 300–1000 ms) inhibition, and less facilitation (IPI = 50–150 ms) when compared to the 90-day old adult rats during all three vigilance states. PPI values obtained from the 30-day old group fell intermediate between the 15- and 90-day old animals. These changes in PPI values provide a quantitative measure of changes in the modulation of dentate granule cell excitability during normal maturation. They can now can be used to evaluate the impact of various insults, such as prenatal protein malnutrition or neonatal stress, on hippocampal development. © 2000 Biomedical Engineering Society. PAC00: 8717Nn, 8719La, 8719Nn
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  • 26
    ISSN: 1573-9686
    Keywords: Heart ; Left ventricle ; LV contractility ; ESPVR ; Pig ; Rat ; Magnetic resonance imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The end systolic pressure–volume relation (ESPVR) has been shown to be a relatively load independent measure of left ventricular (LV) contractility. Recently, several single-beat ESPVR computation methods have been developed, enabling the quantification of LV contractility without the need to alter vascular loading conditions on the heart. Using a single-beat ESPVR method, which has been validated previously in humans and assumes that normalized elastance is constant between individuals of a species, we studied the effects of myocardial infarction on LV contractility in two species, the rat and the pig. In our studies, LV pressure was acquired invasively and LV volume determined noninvasively with magnetic resonance imaging, at one week postinfarction in pigs and at 12 weeks postinfarction in rats. Normalized systolic elastance curves in both animal species were not statistically different from that of humans. Also, the slope of the ESPVR $$\left( {E_{es} } \right)$$ decreased significantly following infarction in both species, while the volume-axis intercept $$\left( {V_0 } \right)$$ was unaffected. These results indicate that a single-beat ESPVR method can be used to measure the inotropic response of the heart to myocardial infarction, and that the basis for this method (i.e., constant normalized elastance) is applicable to a variety of mammalian species. © 2000 Biomedical Engineering Society. PAC00: 8719Uv, 8761Lh, 8719Hh, 8719Rr, 8719Ff
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  • 27
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    Annals of biomedical engineering 28 (2000), S. 1101-1115 
    ISSN: 1573-9686
    Keywords: Time–frequency analysis ; Coherence ; Cross correlation ; Nonstationary persistent signals ; Central pattern generator ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract We present a novel time-varying phase spectrum (TVPS) method to quantify the dynamics of coevolution of two persistent nonstationary coupled signals. Based on the TVPS, an instantaneous intersignal phase shift is defined within the primary frequency range in which the two signals are highly correlated. The TVPS is estimated using a fixed-window method or an adaptive-window method. In the latter method, the window length changes dynamically and automatically as a function of change in frequency of the signals. The effects of altering window types and lengths on the accuracy of the estimation of the primary phase shift is assessed by analyzing synthesized linear chirp signals with decaying amplitude and constant relative phase shift or decaying amplitude and changing relative phase shifts. The methods developed are also used for determining the evolution of the primary phase shift among ventral root activities during fictive locomotion in an in vitro rat spinal cord preparation. The analyses indicate that the TVPS method in conjunction with the determination of the primary frequency range, allows determination of both the evolution of the coupling strength and the evolution of the phase shift between two persistent nonstationary rhythmic signals in the joint time–frequency domain. An adaptive window reduces the estimation bias and the estimation variability. © 2000 Biomedical Engineering Society. PAC00: 0230-f, 8780Tq
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  • 28
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    Plant and soil 226 (2000), S. 11-19 
    ISSN: 1573-5036
    Keywords: Chlorpropham (CIPC) ; microtubules ; nuclei ; recovery ; roots ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The present ultrastructural investigation on the effects of 50 μM chlorpropham (previously called CIPC) on growing roots of wheat (Triticum aestivum (L.) Thell cv. Vergina) was undertaken to clarify the mechanism of a carbamate herbicide action in plant cells, since the wide range of responses of plant cells to carbamate herbicides is based mainly on immunofluorescence studies. Cells of control roots contained abundant microtubules both in interphase and mitotic arrays. In chlorpropham-treated roots, however, no microtubules could be detected at all, neither in dividing nor in differentiating cells. Cycling cells became binucleate, polyploid or contained incomplete cell walls, the result of inhibition of cytokinesis. In long-term drug treatments (24 h or more) the affected cells entered a new cycle, which, however, did not progress beyond mid-metaphase. The nuclei of binucleate cells initiated prophase synchronously. Small vacuoles and Golgi vesicles were trapped within the nucleoplasm of the multilobed nuclei. In roots recovering from 8 h chlorpropham treatment, cells continued to exhibit polyploid nuclei, intranuclear vacuoles and incomplete walls. Microtubules reappeared but they were sparse and lacked a definite orientation. Preprophase cells did not form normal preprophase bands of microtubules, while mitotic cells occasionally contained microtubules bound to chromosomes and converged to minipoles. It is concluded that chlorpropham disorganized directly microtubules in addition to irreversibly affecting microtubule organizing centres, which failed to further support microtubule arrays.
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  • 29
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    Plant and soil 226 (2000), S. 275-285 
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza ; Daucus carota ; Glomus mosseae ; Glomus intraradices ; monoxenic culture ; N uptake ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract New information on N uptake and transport of inorganic and organic N in arbuscular mycorrhizal fungi is reviewed here. Hyphae of the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe (BEG 107) were shown to transport N supplied as 15N-Gly to wheat plants after a 48 h labelling period in semi-hydroponic (Perlite), non-sterile, compartmentalised pot cultures. Of the 15N supplied to hyphae in pot cultures over 48 h, 0.2 and 6% was transported to plants supplied with insufficient N or sufficient N, respectively. The increased 15N uptake at the higher N supply was related to the higher hyphal length density at the higher N supply. These findings were supported by results from in vitro and monoxenic studies. Excised hyphae from four Glomus isolates (BEG 84, 107, 108 and 110) acquired N from both inorganic (15NH4 15NO3, 15NO3 − or 15NH4 +) and organic (15N-Gly and 15N-Glu, except in BEG 84 where amino acid uptake was not tested) sources in vitro during short-term experiments. Confirming these studies under sterile conditions where no bacterial mineralisation of organic N occurred, monoxenic cultures of Glomus intraradices Schenk and Smith were shown to transport N from organic sources (15N-Gly and 15N-Glu) to Ri T-DNA transformed, AM-colonised carrot roots in a long-term experiment. The higher N uptake (also from organic N) by isolates from nutrient poor sites (BEG 108 and 110) compared to that from a conventional agricultural field implied that ecotypic differences occur. Although the arbuscular mycorrhizal isolates used contributed to the acquisition of N from both inorganic and organic sources by the host plants/roots used, this was not enough to increase the N nutritional status of the mycorrhizal compared to non-mycorrhizal hosts.
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  • 30
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    Plant cell, tissue and organ culture 63 (2000), S. 35-40 
    ISSN: 1573-5044
    Keywords: anther culture ; EDTA ; ferrous ions ; ferric ions ; Hordeum vulgare ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A suitable form of iron supplement in the induction medium was found to be important for further development of induced pollen embryos in barley and wheat cultivars (genotypes), especially those providing few green plants viain vitro androgenesis. Genotypes able to regenerate many green plants were less susceptible to the lack of iron in induction medium. Although Fe-EDTA was found to be a suitable form of iron in the induction medium, androgenesis was also induced on media containing non-chelated iron (Fe2+ and Fe3+ ions). EDTA alone without iron inhibited the androgenic response even in the wheat cv. Florida, a model cultivar for androgenesis in wheat. In all barley cultivars under study including cv. Igri, a model cultivar for androgenesis in barley, EDTA alone caused an almost total suppression of androgenesis.
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  • 31
    ISSN: 1573-5060
    Keywords: Cheyenne ; polymorphism ; RAPD ; recombinant inbred chromosome line(RICL) RFLP ; STS ; SSR ; Triticum aestivum ; Wichita
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Previously chromosome 3A of wheat (Triticum aestivum L.) was reported to carry genes influencing yield, yield components, plant height, and anthesis date. The objective of current study was to survey various molecular marker systems for their ability to detect polymorphism between wheat cultivars Cheyenne(CNN) and Wichita (WI), particularly for chromosome3A. Seventy-seven `sequence tagged site' (STS), 10simple sequence repeat (SSR), 40 randomly amplified polymorphic DNA (RAPD) markers, and 52 restriction fragment length polymorphism (RFLP) probes for wheat homoeologous group 3 chromosomes, were investigated. Three (3.9%) STS-PCR primer sets amplified polymorphic fragments for the two cultivars, of which one was polymorphic for chromosome 3A. Sixty percent of SSR markers detected polymorphism between CNN and WI of which 50% were polymorphic for chromosome 3A. Twenty percent of RAPD markers detected polymorphism between CNN and WI in general, but none of these detected polymorphism for chromosome 3A. Of the fifty-two RFLP probes, 78.8% detected polymorphism between CNN and WI for group 3 chromosomes with one or more of seven restriction enzymes and 42% of the polymorphic fragements were for chromosome 3A. These high levels of RFLP and SSR polymorphisms between two related wheat cultivars could be used to map and tag genes influencing important agronomic traits. It may also be important to reconsider RFLP as the most suitable marker system at least for anchor maps of closely related wheat cultivars.
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  • 32
    ISSN: 1573-5060
    Keywords: complementary genes ; hybrid necrosis ; spring wheat ; Triticum aestivum ; winter wheat ; winter × springwheat hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The distribution and allelic expressivity of hybrid necrosis genes (Ne 1 and Ne 2) were studied in 21 winter (mostly exotic) and 43 spring type elite wheat genotypes, by crossing them with two known testers, C 306 (Ne 1-carrier) and HD 2380 (Ne 2-carrier).Ne 1 gene was present in one north-west Himalayan winter wheat landrace, Shoure Local, but absent in the other winter as well as spring wheats. Ne 2 gene was prevalent to a much lower extent in the exotic winter wheat germplasm (31.57%) as compared to the recently developed Indian and Mexican spring wheat semidwarfs (69.80%). This may suggest that breeders have tried to preclude hybrid necrosis by selecting for non-carrier genotypes in the development of exotic winter wheats in contrast to the situation in spring wheats. Based on the degree of expression of hybrid necrosis genes in the F1 hybrids, the carrier genotypes were characterized with respect to the allelic strength of the hybrid necrosis genes. The 27 non-carrier genotypes of the two ecotypes identified in the present study have a greater potential use in future hybridization programmes so as to overcome the problem of hybrid necrosis.
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  • 33
    ISSN: 1573-5060
    Keywords: allelic variation ; Chinese wheat ; glutenin subunit ; seed storage protein ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Variation in the electrophoretic banding patterns of high-molecular-weight (HMW) glutenin subunits of 274hexaploid wheat (Triticum aestivum) varieties from China was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and 27 different major HMW glutenin subunits were identified. Each variety contained three to five subunits and 29different glutenin subunit patterns were observed in274 Chinese hexaploid wheats. Seventeen alleles were identified based on the comparison of subunits mobility with that previously identified in a set of standard hexaploid wheats. The Chinese hexaploid wheats exhibited allelic variation in HMW glutenin subunit composition and the variation differed from that of Japanese and hexaploid wheats of other countries.
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  • 34
    ISSN: 1573-5060
    Keywords: bunt infection ; bunt resistance ; frost resistance ; Tilletia caries ; T. foetida ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to determine the effects of bunt inoculation on frost resistance and winter hardiness in lines containing resistance genes, the bunt [Tilletia foetida (Wallroth) Liro, T. caries (DC.) Tulasne] susceptibility of wheat lines containing bunt resistance genesBt1 to Bt10 and the effect of the year on the degree of infection were studied over six years from 1991 to 1997 in an artificial inoculation nursery. Uninoculated and artificially inoculated wheat plants were tested for frost resistance in the phytotron in 1995 and in the field in boxes in three years from 1994/95 to 1996/97. The line withBt10 was very resistant, lines with Bt5, Bt6, Bt8 and Bt9 were resistant, the line with Bt4 was moderately resistant, those with Bt2 and Bt3 were moderately susceptible, the line with Bt1 was susceptible and the line with Bt7 was very susceptible to the local bunt population in Hungary. Bunt incidence also varied over years. The frost resistance of the Bt lines was generally lower after bunt inoculation than that of uninoculated plants. The increased frost kill in inoculated plants was not correlated with the extent of varietal susceptibility to bunt. Some lines with resistance, namely those with Bt5 (1.6% infection), Bt8 (0.6%) and Bt10 (0.0%), suffered significantly greater frost kill in the young plant stage as the result of bunt inoculation. By contrast, the Bt7line had excellent frost resistance and winter hardiness but suffered the greatest extent of bunt infection, whereas the Bt6 line had good frost resistance and good bunt resistance.
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  • 35
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    Plant and soil 222 (2000), S. 25-34 
    ISSN: 1573-5036
    Keywords: deficiency ; genotypic differences ; iron ; nutrient efficiency ; phytosiderophore ; tolerance ; Triticum aestivum ; Triticum durum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tolerance to Zn deficiency in wheat germplasm may be inversely related to uptake and transport of Fe to shoots. The present study examined eight bread (Triticum aestivum) and two durum (T. turgidum L. conv. durum) wheat genotypes for their capacity to take up and transport Fe when grown under either Fe or Zn deficiency. Bread wheat genotypes Aroona, Excalibur and Stilleto showed tolerance to Zn and Fe deficiency, while durum wheat genotypes are clearly less tolerant to either deficiency. Roots of bread wheats tolerant to Zn deficiency exuded more phytosiderophores than sensitive bread and durum genotypes. Greater amounts of phytosideophores were exuded by roots grown under Fe than Zn deficiency. A relatively poor relationship existed between phytosiderophore exudation or the Fe uptake rate and relative shoot growth under Fe deficiency. At advanced stages of Zn deficiency, genotypes tolerant to Zn deficiency (Aroona and Stilleto) had a greater rate of Fe uptake than other genotypes. Zinc deficiency depressed the rate of Fe transport to shoots in all genotypes in early stages, while advanced Zn deficiency had the opposite effect. Compared with Zn-sufficient plants, 17-day-old Zn-deficient plants of genotypes tolerant to Zn deficiency had a lower rate of Fe transport to shoots, while genotypes sensitive to Zn deficiency (Durati, Yallaroi) had the Fe transport rate increased by Zn deficiency. A proportion of total amount of Fe taken up that was transported to shoots increased with duration of either Fe or Zn deficiency. It is concluded that greater tolerance to Zn deficiency among wheat genotypes is associated with the increased exudation of phytosiderophores, an increased Fe uptake rate and decreased transport of Fe to shoots.
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  • 36
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    Euphytica 112 (2000), S. 23-31 
    ISSN: 1573-5060
    Keywords: A granules ; B granules ; quantitative analysis ; starch quality ; triploid endosperm ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two lines of hexaploid wheat were crossed and the basic generations of parent, F1, F2 and back-cross were sown in a controlled-environment chamber. FreshF1 and back-cross grains were generated, so the material could be handled either as the standard set of basic generations on a whole-plant basis, or as an extended set on an embryo or endosperm basis. The experiment was repeated. Mature grains were harvested and the starch particle size distribution was analysed in 3284 grains from 111 plants. Means and variances were partitioned into additive, dominance and interaction components. Grains from cross-pollinations had B-granule contents between parental values, rather than of the maternal parent, indicating an involvement of the grain genotype. Quantitative models based on endosperm genotype gave a better fit to the data than those based on embryo genotype. The difference in starch B-granule content between the parents was largely due to additive genes. Dominant genes were also indicated, with the first dose in the triploid endosperm having a large effect while the second dose had little or none. Non-allelic interactions were significant in the second experiment where the use of more types of backcross made them more detectable. There were also small and significant residual effects of the maternal plant in the first experiment, attributed to the vigour of the F1 mother plant and to the cytoplasm of Sunco. Narrow-sense heritability was low, between 0.05and 0.18 depending on the generation. Transgressive segregation was not found, suggesting that all alleles tending to increase the B-granule content were found in the Sunco parent and none in ME71. There was also no detectable heterosis in this character. The results show that breeding and selection for a low B-granule content should be possible but a further reduction will require new and complementary genes.
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  • 37
    ISSN: 1573-5060
    Keywords: disease resistance ; doubled haploid ; Fusarium headblight ; genetic analysis ; Fusarium graminearum ; recombinant inbred ; Triticum aestivum ; wheat scab
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic constitution of resistance to Fusarium head blight (FHB, scab) caused by Fusarium graminearum in the Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165 was investigated using doubled haploid lines (DHLs) and recombinant inbred lines (RILs). Frequency distributions of DHLs derived from two F1 crosses, Sumai 3 (very resistant to resistant; VR-R) / Gamenya (very susceptible; VS) and Sumai 3 / Emblem (VS), fitted well to 1: 2: 1 (resistant: moderately resistant: susceptible) ratios for reaction to FHB in the field. It is suggested that the resistance of Sumai 3 is controlled by two major genes with additive effects. One of the resistance genes may be linked in repulsion to the dominant suppressor B1 for awnedness with recombination values 15.1 ± 3.3% in Sumai 3 /Gamenya and 21.4 ± 4.3% in Sumai 3 / Emblem. Saikai 165 is a Japanese resistant line derived from an F1 Sumai 3 / Asakaze-komugi (moderately resistant; MR). The data for RILs derived from the cross Emblem / Saikai 165, indicates that three resistance genes control the resistance of Saikai 165.
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  • 38
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    Euphytica 113 (2000), S. 219-225 
    ISSN: 1573-5060
    Keywords: deficiency ; genotypic variation ; iron ; nutrient efficiency ; phytosiderophore ; Triticum aestivum ; Triticum durum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tolerance to Fe deficiency of wheat genotypes exhibiting differential tolerance to Zn deficiency is not known, even though the relationship between Fe nutrition and differential tolerance of wheat genotypes to Zn deficiency has been hypothesised frequently. In the present experiment, eight Triticum aestivum and two T. turigidum L. conv. durum cultivars were grown in nutrient solution deficient in either Znor Fe. Three indices of tolerance to nutrient deficiency were compared: relative [(-nutrient/+nutrient) × 100] shoot growth, shoot dry weight under nutrient deficiency and relative shoot/root dry weight ratio. Genotypes Aroona, Excalibur, Stilleto and Trident were classified as tolerant to both Zn and Fe deficiency, while durum wheats Durati and Yallaroi were sensitive to Zn deficiency and moderate to sensitive to Fe deficiency. Genotypes Excalibur, Stilleto and Trident come from the same breeding programme and have the common parent (line MEC3 =Sonora64//TZPP/YAQUI54) that could have been the donor of the genes for tolerance to Zn deficiency. When Fe-deficient, all wheat genotypes were severely chlorotic but kept producing shoot and root dry matter at a relatively high rate, making the relationship between the relative shoot growth and the relative leaf chlorophyll content poor. This is the first report of wheat genotypes exhibiting multiple tolerance to Zn and Fe deficiencies.
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  • 39
    ISSN: 1573-5060
    Keywords: bread wheat ; breeding and environmental effects ; gene frequency ; geographical distribution ; hybrid necrosis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using the Information and Analytical System of Wheat Genetic Resources GRIS 3.2, the peculiarities of distribution of hybrid necrosis genes in bread wheat in different regions of the world were analyzed. Considerable variation in frequencies of the Ne1 and Ne2 genes in regions with different moisture and heat supply was revealed. A significant effect of breeding on frequency dynamics of different genotypes Ne1ne2, ne1Ne2 and ne1ne2 was confirmed.
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  • 40
    ISSN: 1573-5087
    Keywords: abscisic acid ; drought stress ; Triticum aestivum ; wheat germ agglutinin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Expression of wheat germ agglutinin (WGA) gene inthe developing embryos of wheat (Triticumaestivum L. cv. C-306) was studied in relation toabscisic acid (ABA) accumulation under water stressconditions. Imposition of water stress resulted inelevated ABA levels in the embryos at threedevelopmental stages (18, 24 and 30 DPA). On thecontrary, the effect of drought stress on WGAaccumulation was stage dependent with significantincrease in WGA content being observed at only 24 DPA. Our results suggest that apart from ABA, otherfactors which are temporally expressed, are alsoinvolved in regulation of WGA gene expression.
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  • 41
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    Genetic resources and crop evolution 47 (2000), S. 281-284 
    ISSN: 1573-5109
    Keywords: agronomic traits ; isozymes ; landrace ; Triticum aestivum ; variation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A sample of an Argentinean landrace of wheat showed considerable variation in most of the evaluated morphological and agronomic characters. However, analyses with high molecular glutenins and two isozyme systems, known to be highly polymorphic among current cultivars, revealed very little or no variation, respectively. The large difference in the observed variation between morphoagronomic and biochemical characters is discussed.
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  • 42
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    Journal of chemical ecology 26 (2000), S. 2141-2154 
    ISSN: 1573-1561
    Keywords: Allelopathy ; phenolic acids ; 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one ; DIMBOA ; GC-MS-MS ; wheat ; Triticum aestivum ; weed suppression ; annual ryegrass ; Lolium rigidum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Wheat allelopathy has potential for weed suppression. Allelochemicals were identified in wheat seedlings, and they were exuded from seedlings into agar growth medium. p-Hydroxybenzoic, trans-p-coumaric, cis-p-coumaric, syringic, vanillic, trans-ferulic, and cis-ferulic acids and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) were identified in both the shoots and roots of 17-day-old wheat seedlings and their associated agar growth medium. Wheat accessions with previously identified allelopathic activity tended to contain higher levels of allelochemicals than poorly allelopathic ones. The allelopathic compounds present in the shoots generally also were identified in the roots and in the agar medium. Allelochemicals were distributed differentially in wheat, with roots normally containing higher levels of allelochemicals than the shoots. When the eight allelochemicals were grouped into benzoic acid and cinnamic acid derivatives, DIMBOA, total coumaric, and total ferulic acids, the amount of each group of allelochemicals was correlated between the roots and the shoots. Most of the allelochemicals identified in the shoots and roots could be exuded by the living roots of wheat seedling into the agar growth medium. However, the amounts of allelochemicals in the agar growth medium were not proportional to those in the roots. Results suggest that wheat plants may retain allelochemicals once synthesized. The presence of allelochemicals in the agar growth medium demonstrated that wheat seedlings were able to synthesize and to exude phytotoxic compounds through their root system that could inhibit the root growth of annual ryegrass.
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  • 43
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    Nutrient cycling in agroecosystems 56 (2000), S. 109-116 
    ISSN: 1573-0867
    Keywords: labelled nitrogen ; Lolium perenne ; nitrogen cycling ; root biomass ; straw ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field trial was carried out to compare the transformations and plant uptake of urine N and S in a short-term pasture from within an arable/pasture ley rotation and a long-term pasture. Animal urine labelled with 15N and 35S was applied to microplots at both sites. These microplots were destructively sampled at various time intervals over 12 months and analysed for 15N and 35S. It is known that soil organic matter accumulates under short-term pastures compared with a long-term pasture in which accumulation and degradation are in balance. Consequently, it was hypothesised that immobilization of urine N and S is more intense in the short-term. However, in this study there was considerably less immobilization of 15N and 35S into soil organic forms under short-term pasture than long-term pasture. This was attributable to a greater pasture dry matter response to urine application under the short-term pasture (due to its inherently low N fertility) resulting in a greater plant uptake of 15N and 35S with less 15N and 35S consequently being available for immobilization. At both sites, all of the applied 35S was accounted for through plant uptake and recovery in the soil, but 21–48% of the 15N was unaccounted for and presumed to have been lost through gaseous emissions. It was concluded that accumulation of soil organic N and S under short-term pastures is likely to be attributable to turnover of plant residues (particularly root material) and does not appear to be related to immobilization in urine patches.
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  • 44
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    Plant cell, tissue and organ culture 60 (2000), S. 69-73 
    ISSN: 1573-5044
    Keywords: cereals ; tissue culture ; Triticum durum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile, green plants were regenerated from immature inflorescence explants from each of four Canadian wheat cultivars. The cultivars were representative of four classes of Canadian wheat. Explants from immature inflorescences of three size ranges were cultured on two types of media: MSI/MSR, which contains 1650 mg l-1 NH4NO3and sucrose as a carbon source, and BII/BIR, which contains 250 mg l-1 NH4NO3and maltose as a carbon source. Regeneration from all cultivars was significantly better on BII/BIR media than on MSI/MSR media. On BII/BIR media, `AC Karma', `Plenty', and `Fielder' gave the highest number of shoots per 10 explants, where the explants were derived from immature inflorescences 5.1 to 10.0 mm in length. 'Columbus' did not regenerate on MSI/MSR medium, and regenerated poorly on BII/BIR medium. Differences were found between cultivars with regard to the number of regenerant plants produced with the best treatments: `Plenty' produced 16.1 shoots per 10 explants, `AC Karma' 12.4, `Fielder' 6.4, and `Columbus' 2.2.
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  • 45
    ISSN: 1573-5060
    Keywords: biochemical marker ; gene location ; Lophopyrum elongatum ; Puccinia striiformis ; stripe rust ; substitution line ; Triticum aestivum ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A set of T. aestivum-L. elongatum chromosome substitution lines was tested for yellow rust resistance at the seedling stage. Inheritance of the resistance and esterase-5 (Est-5) variation were studied. The results demonstrated that L.elongatum carried a new gene(s) conferring yellow rust resistance. This gene was dominant and located on chromosome 3E of L. elongatum. The biochemical locus encoding Est-5was also located on chromosome 3E, and co-segregated with theYr gene(s) in the wheat background. The transmission frequencies of chromosome 3E in 3E(3A) × CS, 3E(3B) × CS and 3E(3D) × CS hybrids were scored.None of the hybrids transmitted the alien chromosome at thetheoretical maximum rate, but the transmission frequencies ofchromosome 3E in F2 populations of 3E(3A) × CS and 3E(3D) × CS were significantly higher than in thatof 3E(3B) × CS.
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  • 46
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    Euphytica 111 (2000), S. 199-203 
    ISSN: 1573-5060
    Keywords: Diuraphis noxia (Mordvilko) ; protein ; Russian wheat aphid ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), has become a perennial, serious pest of wheat (Triticum aestivum L.) in the western United States. Current methodologies used to enhance RWA resistance in wheat germplasm could benefit from an understanding of the biochemical mechanisms underlying resistance to RWA. This study was initiated to identify specific polypeptides induced by RWA feeding that may be associated with RWA resistance. The effects of RWA feeding on PI 140207 (a RWA-resistant spring wheat) and Pavon (a RWA-susceptible spring wheat) were examined by visualizing, silver-stained denatured leaf proteins separated by two-dimensional polyacrylamide gel electrophoresis. Comparisons of protein profiles of noninfested and RWA-infested Pavon and PI 140207 revealed a 24-kilodalton-protein complex selectively inhibited in Pavon that persisted in PI 140207during RWA attack. No other significant qualitative or quantitative differences were detected in RWA-induced alterations of protein profiles. These results suggest that RWA feeding selectively inhibit synthesis and accumulation of proteins necessary for normal metabolic functions in susceptible plants.
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  • 47
    ISSN: 1573-5036
    Keywords: plant uptake ; potassium ; potassium starvation ; radiocaesium ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Short term experiments were carried out to investigate the effect of internal tissue potassium concentration on the uptake of radiocaesium by spring wheat (Triticum aestivum cv. Tonic). The results showed that potassium starvation increased Cs influx rates by a factor of 10 compared with non-starved plants. Solution to plant tissue transfer factor (TF) values also increased by around an order of magnitude after potassium starvation treatment. The enhancement of Cs influx rates by potassium starvation could be offset by an increase in external potassium concentration: this effect is minimal at external potassium concentrations greater than approximately 200 μM (8 mg L-1). This reveals that Cs influx into plant roots is subject to control by both internal and external potassium status. The kinetics of Cs uptake by potassium in starved and non-starved plants can be described adequately by the Michaelis-Menten equation. It was shown that potassium starvation substantially reduces the Km value from approximately 28 to 6 μM, which suggests that starvation treatment increases significantly the affinity of plant roots for Cs+. Mechanisms involved in K-Cs interactions during plant uptake are discussed in this paper. Finally, the relevance of such mechanisms as determinants of radiocaesium uptake by plants growing under different ecological conditions is emphasised.
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  • 48
    ISSN: 1573-5060
    Keywords: genetic diversity ; germplasm ; RFLP ; Triticum aestivum ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A set of 292 accessions of common wheat (Triticum aestivum L.) representing 21 germplasm pools based on geographical or breeding program origins was assayed for RFLP diversity. Thirty cDNA and genomic DNA probes and the HindIII restriction enzyme were employed for RFLP analysis. About 61% of all 233 scored bands were present in 75% or more of the accessions. All but one of the 30 probes revealed polymorphism, and the average number of distinct patterns per probe over all accessions was 9.5.Polymorphic Information Content (PIC) values within a pool varied from 0 to 0.9 and depended on the identities of both the germplasm pool and the probe. Rare banding patterns with a relative frequency of ≤0.2 within a pool were detected. These rare patterns were more likely to occur in pools exhibiting high levels of heterogeneity. The highest level of polymorphism was observed in the Turkish landraces from Southwest Asia. The Eastern U.S. soft red winter wheat germplasm pool was more genetically diverse than the other advanced germplasm pools, and nearly as diverse as the Turkish landrace pool. RFLP-based genetic relationships between germplasm pools generally tracked expectations based on common geographical origin, breeding history and/or shared parentages. The Chinese wheat landraces from Sichuan, Tibet, and Yunnan provinces were distinct from other pools. Similarity matrices for among-pool genetic distance estimates based on either band frequencies or banding pattern frequencies showed good correlation with matrices derived from Nei and Li's mean genetic similarity estimates (r=−0.82** and r=−0.73**, respectively.
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  • 49
    ISSN: 1573-5079
    Keywords: canopy architecture ; canopy photosynthesis ; CO2 enrichment ; global change ; leaf area index ; leaf tip angle ; nitrogen stress ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response of whole-canopy net CO2 exchange rate (CER) and canopy architecture to CO2 enrichment and N stress during 1996 and 1997 for open-field-grown wheat ecosystem (Triticum aestivum L. cv. Yecora Rojo) are described. Every Control (C) and FACE (F) CO2 treatment (defined as ambient and ambient +200 μmol mol−1, respectively) contained a Low- and High-N treatment. Low-N treatments constituted initial soil content amended with supplemental nitrogen applied at a rate of 70 kg N ha−1 (1996) and 15 kg N ha−1 (1997), whereas High-N treatments were supplemented with 350 kg N ha−1 (1996 and 1997). Elevated CO2 enhanced season-long carbon accumulation by 8% and 16% under Low-N and High-N, respectively. N-stress reduced season-long carbon accumulation 14% under ambient CO2, but by as much as 22% under CO2 enrichment. Averaging both years, green plant area index (GPAI) peaked approximately 76 days after planting at 7.13 for FH, 6.00 for CH, 3.89 for FL, and 3.89 for CL treatments. Leaf tip angle distribution (LTA) indicated that Low-N canopies were more erectophile than those of High-N canopies: 48° for FH, 52° for CH, and 58° for both FL and CL treatments. Temporal trends in canopy greenness indicated a decrease in leaf chlorophyll content from the flag to flag-2 leaves of 25% for FH, 28% for CH, 17% for CL, and 33% for FL during 1997. These results indicate that significant modifications of canopy architecture occurs in response to both CO2 and N-stress. Optimization of canopy architecture may serve as a mechanism to diminish CO2 and N-stress effects on CER.
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  • 50
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    Chromosome research 8 (2000), S. 671-676 
    ISSN: 1573-6849
    Keywords: meiotic metaphase I pairing ; recombinant isochromosome ; Triticum aestivum ; Triticum dicoccoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A recombinant isochromosome i5BLrec of wheat was developed with one arm and the proximal 36% of the other arm of Chinese Spring (CS) origin and the distal 64% of the recombined arm of Triticum turgidum subsp. dicoccoides origin. The i5BLrec} provides an unusual opportunity to analyze the role of the centromere or arm heterozygosity in chromosome prealignment and synapsis during meiosis. In monosomic condition, the i5BLrec formed a ring univalent in 86.8% of the pollen mother cells (PMCs) at meiotic metaphase I. In the disomic condition, the two i5BLrec preferentially paired as a normal bivalent in 74.8% of the PMCs, which differed significantly (p〈0.01) from the normal bivalent pairing of 51% observed in diisosomic 5BL chromosomes of the CS (Di5BLCS) control plants. In plants with one i5BLrec and a normal 5BCS, the long arm of 5BCS paired with the homologous arm of i5BLrec in 54.4% of the PMCs, and 40.4% of the PMCs had a 5BCS univalent and a i5BLrec ring univalent. The implications of the i5BLrec pairing data on the mechanism of Ph1 gene action are discussed.
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  • 51
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    Chromosome research 8 (2000), S. 501-511 
    ISSN: 1573-6849
    Keywords: BFB cycle ; chromosome healing ; gametocidal factor ; rye deficiencies ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gametocidal factor on the Aegilops cylindrical chromosome 2Cc was used to induce and analyze the nature of chromosomal rearrangements in rye chromosomes added to wheat. For this purpose we isolated plants disomic for a given rye chromosome and monosomic for 2Cc and analyzed their progenies cytologically. Rearranged rye chromosomes were identified in 7% of the progenies and consisted of rye deficiencies (4.6%), wheat–rye dicentric and rye ring chromosomes (1.8%), and terminal translocations (0.6%). The dicentric and ring chromosomes initiated breakage–fusion–bridge cycles (BFB) that ceased within a few weeks after germination as the result of chromosome healing. Of 56 rye deficiencies identified, after backcrossing and selfing, only 33 were recovered in either homozygous or heterozygous condition covering all rye chromosomes except 7R. The low recovery rate is probably caused by the presence of multiple rearrangements induced in the wheat genome that resulted in poor plant vigor and seed set, low transmission, and an underestimation of the frequency of wheat–rye dicentric chromosomes. Genomic in-situ hybridization (GISH) analysis of the 33 recovered rye deficiencies revealed that 30 resulted from a single break in one chromosome arm followed by the loss of the segment distal to the breakpoint. Only three had a wheat segment attached distal to the breakpoint. Although some of the Gc-induced rye rearrangements were derived from BFB cycles, all of the recovered rye rearrangements were simple in structure. The healing of the broken chromosome ends was achieved either by the de-novo addition of telomeric repeats leading to deficiencies and telocentric chromosomes or by the fusion with other broken ends in the form of stable monocentric terminal translocation chromosomes.
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  • 52
    ISSN: 0219-1032
    Keywords: c-Fos ; Dopamine ; D1 ; Hippocampus ; Rat ; Synaptic Plasticity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract While dopamine is likely to modulate hippocampal synaptic plasticity, there has been little information about how dopamine affects synaptic transmission in the hippocampus. The expression of IEGs including c-fos has been associated with late phase LTP in the CA1 region of the hippocampus. The induction of c-fos by dopaminergic receptor activation in the rat hippocampus was investigated by using semiquantitative RT-PCR and immuno-cytochemistry. The hippocampal slices which were not treated with dopamine showed little expression of c-fos mRNA. However, the induction of c-fos mRNA was detected as early as 5 min after dopamine treatment, peaked at 60 min, and remained elevated 5 h after treatment. Temporal profiles of increases in c-fos mRNA by R(+)-SKF-38393 (50 μM) and forskolin (50 μM) were similar to that of dopamine. An increase in [cAMP] was observed in dopamine-, SKF-, or forskolin-treated hippocampal slices. By immunocytochemical studies, control hippocampal cells showed little expression of c-Fos immunoreactivity. However, when cells were treated with dopamine, an increase in the expression of c-Fos immunoreactivity was observed after treatment for 2 h. The treatment of hippocampal neurons with R(+)-SKF38393 (50 μM) or forskolin (50 μM) also induced a significant increase in c-Fos expression. These results indicate that the dopamine D1 receptor-mediated cAMP dependant pathway is associated with the expression of c-Fos in the hippocampal neurons. These data are consistent with the possible role of endogenous dopamine on synaptic plasticity via the regulation of gene expression. Furthermore, these results imply that dopamine might control the process of memory storage in the hippocampus through gene expression.
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  • 53
    ISSN: 1573-9058
    Keywords: chlorophyll fluorescence ; diphenyl carbazide ; donor side ; electron transport ; MnCl2 ; NH2OH ; photosystem ; senescence ; thylakoid protein ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in various components of photosynthetic apparatus during the 6-d dark incubation at 25 °C of detached control and DCMU-treated Triticum aestivum L. leaves were examined. The rate of photosystem 2 (PS2) activity was decreased with increase of the time of dark incubation in control leaves. In contrast to this, DCMU-treated leaves demonstrated high stability by slowing down the inactivation processes. Diphenyl carbazide and NH2OH restored the PS2 activity more in control leaves than in DCMU-treated leaves. Mn2+ failed to restore the PS2 activity in both control and DCMU-treated samples. Similar results were obtained when Fv/Fm was evaluated by chlorophyll fluorescence measurements. The marked loss of PS2 activity in dark incubated control leaves was primarily due to the loss of D1, 33, and 23 kDa extrinsic polypeptides and 28-25 kDa LHCP2 polypeptides.
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  • 54
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    Photosynthetica 37 (2000), S. 519-527 
    ISSN: 1573-9058
    Keywords: alanine ; aspartate ; glycine ; glycollate ; malate ; nitrate ; serine ; sugars ; Triticum aestivum ; urea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 14CO2 uptake in leaves of wheat plants (Triticum aestivum L.) fertilized by urea or Ca(NO3)2 (25 mol m-3) was investigated. The Warburg effect (inhibition of 14CO2 uptake by oxygen) under 0.03 vol. % CO2 concentration was observed only in non-fertilized plants. Under 0.03 vol. % CO2, the Warburg antieffect (stimulation of 14CO2 uptake by oxygen) was detected only in plants fertilized by Ca(NO3)2. Under saturating CO2 concentration (0.30 vol. %), the Warburg antieffect was observed in all variants. Under limitation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity (0.30 vol. % CO2 + 1 vol. % O2), the rate of synthesis of glycollate metabolism products decreased in control and urea-fertilized plants but was enhanced in nitrate-fed plants. Hence, there was an activation of glycollate formation via transketolase reaction in fertilized plants, and the products of nitrate reduction function were oxidants in nitrate-fertilized plants whereas the superoxide radical played this role in urea-fertilized plants.
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  • 55
    ISSN: 1573-9058
    Keywords: net photosynthetic rate ; soil and atmospheric drought ; stomatal conductance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Yields of wheat in semiarid and arid zones are limited by drought, and water condition is very important at each stage of development. Studies carried out at Loess Plateau in the northwestern part of China indicated that yield of spring wheat (Triticum aestivum L.) cv. Dingxi 81-392 was reduced by 41% when subjected to water stress. The effects of two water regimens on net photosynthetic rate (P N), stomatal conductance (g s), and intercellular CO2 concentration (C i) were investigated at the jointing, booting, anthesis, and grain filling stages. Low soil moisture in comparison to adequate one had invariably reduced P N during the diurnal variations at the four growth stages. P N and g s in both soil moisture regimes was maximally reduced at midday. C i and the stomatal limitation fluctuated remarkably during photosynthesis midday depression processes, especially at the grain filling stage. Hence atmospheric drought at midday was one of the direct causes inducing stomata closure and the g s depression, but it was beneficial for maintaining stable intrinsic water use efficiency. Fluctuation in C i implicated that non-stomatal limitation also plays an important role during the period of photosynthesis midday depression. Consequently stomatal and/or non-stomatal limitation are the possible cause of the midday photosynthesis decline.
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  • 56
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    Photosynthetica 37 (2000), S. 615-619 
    ISSN: 1573-9058
    Keywords: carbon based secondary compounds (CBSC) ; isoorientin ; leaf age ; litter ; phenolics ; tricin ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We compared flavonoids in green, mature, and senescing flag leaves of wheat grown under ambient (AC - 370 μmol mol-1) and elevated (EC - 550 μmol mol-1) concentrations of CO2 in a FACE (Free Air CO2 Enrichment) system. The concentrations of flag leaf flavonoids (e.g., isoorientin and tricin) decreased to one third in mature leaves, and the majoritary isoorientin almost disappeared in senescing leaves. Flavonoid concentrations increased in green well-developed flag leaves under EC (46 % isoorientin and 55 % tricin), whereas the differences disappeared in mature and senescing flag leaves. Predictions of changes in litter phenolic concentrations and their effects on decomposition rates under EC based on changes in green leaves need to be revised.
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  • 57
    ISSN: 1434-193X
    Keywords: Triplet recombination ; Electron transfer ; Radical ions ; Photochemistry ; Terpenes ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The triphenypyrylium tetrafluoroborate (TPT)-sensitized reactions of several terpene donor molecules, including sabinene (1), α-phellandrene (4), α-terpinene (5) and γ-terpinene (6) give rise to significantly different products than reactions induced by other electron-transfer sensitizers, such as 1,4-dicyanobenzene (DCB). The divergent reactions require decidedly different key intermediates; the products obtained with TPT can be explained by dissociative recombination of the intermediate radical-radical cation pair in the triplet state, generating donor-derived biradicals.
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  • 58
    ISSN: 1434-193X
    Keywords: Azides ; Cleavage reactions ; Cycloadditions ; Nitrogen heterocycles ; Polycycles ; Ring expansion ; Synthetic methods ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---2-Alkyl-1-methylquinazolinium hexafluorophosphates 9 are deprotonated by sodium or potassium hydride to afford solutions of 2-alkylidenedihydroquinazolines 10, which were investigated by NMR spectroscopy. Trapping with methanesulfonyl azide (5a) of 10 in situ or subsequent treatment with trifluoromethanesulfonyl azide (5b) gives mixtures of colourless (15) and intensely yellow N-sulfonylimino-1,4-benzodiazepines 16 along with products due to cleavage of the exocyclic double bond of 10, viz. 11 and 13. The ethylidene compound 10b yields the bicyclic products 18 and 19, apparently by complex sequences of reactions that are triggered by removal of the acidic proton at C-2 of 16b and 16f. The structures of the products are based on spectroscopic evidence and X-ray diffraction analyses performed on 15b, 16d, 16e, and 19.
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  • 59
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    Liebigs Annalen 2000 (2000), S. 1589-1593 
    ISSN: 1434-193X
    Keywords: Azaallenium ions ; Azaallylium ions ; Iminium ion ; Kinetics ; Linear Free Energy Relationships ; Ab initio calculations ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The rate constants for the reactions of the 2-azaallenium ion 1b+, the 2-azaallylium ion 2a+ and the iminium ion 3+ with different nucleophiles were determined by 1H NMR spectroscopy. By correlation with the Linear Free Enthalpy Relationship (LFER) lg k20°C = s (E + N), developed by Mayr and Patz, the electrophilicity parameters E(1b+) = -3.7, E(2a+) ≍ -16 and E(3+) = -10.43 were obtained. They show that the relative reactivities of these ions are approximately 1012:1:106. Quantum chemical calculations (ab initio, DFT) of the methyl anion affinities for the ions 1b+,2a+ and3+ are in agreement with the experimental E values. The X-ray structure of 3+·CF3SO3- is reported for the first time; it shows no strong interaction between the cation and the anion.
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  • 60
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    Liebigs Annalen 2000 (2000), S. 1595-1601 
    ISSN: 1434-193X
    Keywords: Oxazoline N-oxide ; Cycloadditions ; Cycloadditions ; Lactams ; Thienamycin ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---[3+2] Cycloaddition between a camphor-derived oxazoline N-oxide 9 and the γ,δ-unsaturated enamino ester 11 afforded the single adduct 6. A stereoselective reduction of the enamino ester side chain allowed the control of the absolute configuration of the two additional asymmetric centres. Nitrogen protection and oxidative hydrolysis of the resulting product 13, followed by further functional group manipulations, led to the β-lactam derivative 1, a known precursor of the β-methylthienamycin derivative2a.
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  • 61
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    Liebigs Annalen 2000 (2000), S. 1603-1607 
    ISSN: 1434-193X
    Keywords: Boron ; Cyclotrimerizations ; Nitrogen heterocycles ; Macrocycles ; Subphthalocyanines ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The regioselective preparation of ortho-substituted subphthalocyanides was achieved employing 3-substituted phthalonitrile derivatives as starting materials. A mechanistic proposal has been outlined.Supporting information for this article is available on the WWW under //http://www.wiley-vch.de/contents/jc_2046/2000/99525_s.pdf or from the author.
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  • 62
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    Liebigs Annalen 2000 (2000), S. 1609-1615 
    ISSN: 1434-193X
    Keywords: Terpenoids ; Natural products ; Total synthesis ; Cyclizations ; Rearrangements ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---A new route for the synthesis of 2,7- and 7-functionalized labdanes starts from (R)-carvone (1). 11-Nordrim-7-en-9-one (15) is an appropriate starting material for the total synthesis of hispanone (21), a biologically active furolabdane isolated from the Mediterranean medicinal plant Ballota saxatilis.
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  • 63
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    Liebigs Annalen 2000 (2000), S. 1623-1626 
    ISSN: 1434-193X
    Keywords: Iridoid glucoside ; (8S)-Kingiside ; (8S)-Loganin ; (8S)-7-Ketologanin ; Asymmetric synthesis ; Natural products ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The tetraacetyl derivative 8 of the naturally occurring kingiside (8a) was prepared from aucubin (1). Intermediates in the synthesis were (8S)-tetraacetyl loganin (6) and (8S)-tetraacetyl-7-ketologanin (7), whose free (8R)-epimers occur in many different plants (Caprifoliaceae, Loganiaceae). The 13C NMR spectrum allows the structure to be unequivocally identified.
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  • 64
    ISSN: 1434-193X
    Keywords: Tetrakis(dimethylamino)naphthalenes ; Basicity ; Hydrogen bonds ; Cyclicvoltammetry ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---For comparison to the recently described 2,3,6,7-tetrakis(dimethylamino)naphthalene (1) the three isomers 2,3, and 4 were synthesized. The basicities of this group of isomers are strongly dependent upon the different mutual orientations of the pairs of dimethylamino substituents: only the isomers 3 and, partially, 4, both with dimethylamino groups in adjacent peri-positions of the naphthalene, are strong “proton sponges”. For the isomers 1 and 2 with the same number and kind of twofold dimethylamino substituents in neighbouring ortho-positions, however, no significant basicity increase is observed. To explain this difference between the two groups of isomers it is suggested that in the ortho-pairs of 1 and 2 the C-N bonds diverge considerably, leading to an increased N···N distance and consequently to less stable [N···H···N]+ hydrogen bonds in contrast to the parallel C-N bonds in the peri-substituted isomers 3 and 4. X-ray crystal structure analyses of the bases and of some of the salts derived therefrom were solved and are discussed. Cyclic voltammetry indicates that 1 to 4 are strong electron donors, reacting easily to radical cations or dications which with suitable acids have been obtained as salts.
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  • 65
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    Liebigs Annalen 2000 (2000), S. 1677-1683 
    ISSN: 1434-193X
    Keywords: Radicals ; Cyclizations ; Pyridinethione ; Tetrahydrofurans ; Asymmetric synthesis ; Thiazolethione ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The trisubstituted functionalized tetrahydrofurans 10, 11, 16, 18, and 19 were photochemically prepared from 2,3-syn- and 2,3-anti-configuredN-(3-benzoyloxy-5-hexen-2-oxy)thiazole-2(3H)-thione anti-6, pyridinethiones 7, anti-8, and BrCCl3. The formation of tetrahydrofurans was achieved by an efficient and highly regioselective alkoxyl radical cyclization (5-exo-trig). The 2,3-anti substituted intermediates 9 and 12 cyclize stereoselectively whereas a 2,3-syn-configured O-radical affords both possible diastereomeric addition products in equal amounts. The cyclized tetrahydrofuryl methyl radicals were trapped with the bromine atom donor BrCCl3 to afford the bromomethyl-substituted cyclic ethers 10, 11, 18, and 19 in excellent yields. The utility of this reaction was stressed by conversion of one of the newly prepared tetrahydrofurans in a two-step synthesis into (+)-allo-muscarine (+)-20.Supporting information for this article is available on the WWW under //http://www.wiley-vch.de/contents/jc_2046/2000/99590_s.pdf or from the author.
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  • 66
    ISSN: 1434-193X
    Keywords: Thioketones ; Thiocarbonyl ylides ; Cycloadditions ; Cycloreversions ; Sulfur heterocycles ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Reactions of diaryl thioketones with diazomethane at room temperature afford 4,4,5,5-tetraaryl-1,3-dithiolanes; the scope of this surprising 2:1 interaction has been studied for decades (Schönberg Reaction). The clue to the mechanism was our observation that the stoichiometry is 1:1 at -78 °C, and 2,5-dihydro-2,2-diaryl-1,3,4-thiadiazoles are formed as primary [2+3] cycloadducts. They lose N2 at -45 °C in first-order reactions generating diaryl thioketone S-methylides which can be intercepted by thioketones (→1,3-dithiolanes), multiple CC bonds, or acids HX. In the absence of trapping reagents, the elusive intermediates either dimerize furnishing 2,2,3,3-tetraaryl-1,4-dithianes or give rise to 2,2-diarylthiiranes by electrocyclization. Beyond thiobenzophenone and diazomethane, our main model reaction, the studies involve fluorene-9-thione, 4,4-dimethoxy- and 4,4-dichlorothiobenzophenone. The ring of 2,5-dihydro-2,2-diphenyl-1,3,4-thiadiazole (8) is opened by LDA at -78 °C and derivatives of anion 12 are obtained. - In summa: The Schönberg reaction consists of two 1,3-dipolar cycloadditions, linked by a 1,3-dipolar cycloreversion.
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  • 67
    ISSN: 1434-193X
    Keywords: Conjugation ; Boron ; Sensors ; Cyclic voltammetry ; Polymers ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---New electropolymerizable aromatic compounds (i.e. pyrrole, thiophene, aniline) bearing boronic acid and ester substituents have been synthesized and their electrochemical behavior has been investigated. Functionalized polythiophene and polypyrrole films could be anodically generated in acetonitrile, whereas the polyaniline derivative was electroformed in an acidic aqueous solution. The electrochemical responses of some of these materials were changed when fluoride ions were added to the electrolytic solutions. The strongest modifications, caused by binding of fluoride by the immobilized boron, were observed for the polypyrrole derivative in hydroorganic media.
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  • 68
    ISSN: 1434-193X
    Keywords: Heterocycles ; Zinc ; Palladium ; Catalysts ; Sulfur ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Organozinc halides derived from Grignard reagents behave differently in their reaction with ethyl (±±)-(2RS,3SR)-tetrahydro-4-methylene-2-phenyl-3-(phenylsulfonyl)furan-3-carboxylate (3) according to the hybridisation of the carbon ligand. During the development of short multi-component reactions for the synthesis of diverse functionalized ethyl 2,5-dihydrofuran-3-carboxylates it was discovered that aryl and vinyl zinc halides undergo clean reaction with 3 in the presence of Pd(PPh3)4. In contrast, when alkyl zinc halides are reacted with 3 in the presence of Pd(PPh3)4, reductive desulfonation of 3 is observed. Remarkably, in the absence of a transition metal catalyst, the allylic substitution of 3 with alkyl zinc halides proceeds cleanly and in moderate to good yield.
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  • 69
    ISSN: 1434-193X
    Keywords: Self-assembly ; Vapor-pressure osmometry ; Resorcin[4]arenes ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Resorcin[4]arene tetracarboxylic acids 5,6 (A) and resorcin[4]arene tetrapyridines 2,3 (P) self-assemble in chloroform solution to form stable heterotopic AP dimers. Data from NMR titration and dilution experiments, as well as from vapor-pressure osmometry (VPO), indicate that the AP dimer is formed with an association constant greater than 107 M-1. Solid-solution extraction experiments are indicative of the formation of a 2:1 trimer (A2P), while self-associated homotopic species (A2 and A3) can be detected by NMR and VPO. Analysis of the heterotopic noncovalent assembly process over a range of compositions shows that these other species are much less stable than the AP heterodimer, which is the exclusive species at an A/P concentration ratio of 1:1 (〉 99.7% of the total at 10 mM).
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  • 70
    ISSN: 1434-193X
    Keywords: Sulfoxides ; β-Amino sulfones ; Mannich type reaction ; Lithium perchlorate ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The LiClO4-mediated one-pot reaction of aldehydes with (trimethylsilyl)dialkyl amines and the lithium salt of sulfoxides or sulfones, affords the corresponding β-(dialkylamino) sulfoxides and β-(dialkylamino) sulfones in high yields. The aminosulfoxidation reaction of aliphatic or aromatic aldehydes lacks diastereoselectivity, but the diastereomeric sulfoxides can be separated by HPLC or column chromatography for further use.
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  • 71
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    Liebigs Annalen 2000 (2000), S. 1741-1744 
    ISSN: 1434-193X
    Keywords: Bridged pyranose derivatives ; Ring enlargement ; Carbenes ; Glycosides ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Addition of dichlorocarbene to the glycal (±±)-2 followed by cyclopropyl-allyl rearrangement leads to the chloro-2H-pyran (±±)-4. Oxidation of (±±)-4 and reduction of the obtained hydroxypyranone (±±)-5 gave the methyl pyranoside (±±)-6. The relative configuration of (±±)-6 was established by X-ray structural analysis of the corresponding acetate (±±)-7. The synthesis of the optically active starting materials (+)-2 and (-)-2 is also reported.
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  • 72
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    Liebigs Annalen 2000 (2000), S. 1745-1758 
    ISSN: 1434-193X
    Keywords: CMP-Neu5Ac analogues ; Enzyme inhibitors ; Substrate analogues ; Transition state analogues ; Transferases ; Carbohydrates ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Quinic acid was transformed into phosphitamides 16, 25, and 36, which could be readily linked to 5′-O-unprotected cytidine derivative 17. Ensuing oxidation of the obtained phosphite triesters with tBuO2H and hydrogenolytic de-O-benzylation furnished the corresponding phosphate diesters 18, 26, and 38. Base catalyzed removal of acetyl protecting groups, and methyl ester hydrolysis furnished CMP-Neu5Ac analogues 1d, 1e, and 2. Quinic acid was also transformed into 1,2-unsaturated diallyl α-hydroxymethyl-phosphate derivatives (R)- and (S)-46, which on reaction with cytidine phosphitamide 47 afforded the phosphite triesters. Subsequent oxidation with tBuO2H and then treatment with NEt3 gave phosphate diester derivatives (R)- and (S)-48. Deallylation, acetyl group removal, and methyl ester hydrolysis furnished (R)- and (S)-3, respectively. Treatment of (R)- and (S)-48 with DBU as a base led to acetic acid elimination, thus yielding, after de-O-allylation, acetyl group cleavage, and ester hydrolysis, diene derivative (E)-4. Donor substrate analogues 1d and 1e exhibited good α(2-6)-sialyltransferase inhibition (Ki: 2.0·10-4 and 2.0·10-5 M). However, transition state analogues (R)-, and particularly (S)-3 showed excellent inhibition properties (Ki: 1.6·10-6 and 2.7·10-7 M).
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  • 73
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    Liebigs Annalen 2000 (2000), S. 1759-1765 
    ISSN: 1434-193X
    Keywords: Cyclitols ; Aminocyclitols ; Glycosidase inhibitors ; α-Mannosidase inhibitors ; Deoxygenation ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Three deoxy derivatives 2-4 of the α-mannosidase inhibitor mannostatin A (1) were synthesized, and their inhibition of Jack bean α-mannosidase was evaluated in order to elucidate the roles of each of the three hydroxyl groups of the inhibitor. The 1- and 2-deoxy derivatives 2 and 3 retained some inhibitory activity, although reduced by a factor of about 100 relative to the parent, whereas it was completely lost with the 3-deoxy derivative 4. Structure and activity relationships are discussed in the light of these findings.
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  • 74
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    Liebigs Annalen 2000 (2000), S. 1767-1772 
    ISSN: 1434-193X
    Keywords: Circular dichroism ; Conformation analysis ; s-Triazines ; Chiral auxiliaries ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---CD data of the optically pure 2-[(R)-1-(9-anthryl)ethylamino]-4-chloro-6-[(R)-1-(1-naphthyl)ethylamino]-1,3,5-triazine, 2[(R)-1-(9-anthryl)ethylamino]-4,6-bis[(R)-1-(1-naphthyl)ethylamino]-1,3,5-triazine, 2,4-bis[(R)-1-(9-anthryl)ethylamino]-6-chloro-1,3,5-triazine are presented. The analysis of the CD spectra by means of the nonempirical DeVoe approach has afforded the complete conformational characterisation of the three s-triazine derivatives, allowing us to establish how the conformation of these derivatives depends on the nature of the substituent 1-arylethylamino groups.
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  • 75
    ISSN: 1434-193X
    Keywords: Cycloadditions ; Carbenes ; Nitrones ; Nitrilimines ; Pyrazolines ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The reaction of tert-butylalkynyl chromium Fischer carbene complex 1 with nitrones 2 affords β-enamino-ketoaldehydes 4 by the light-promoted rearrangement of the corresponding [3+2] cycloadduct carbene complexes 3. On the other hand, [3+2] cycloaddition of chiral nonracemic Fischer alkenyl carbene complexes 19 with nitrilimines 10 yields enantiomerically pure Δ2-pyrazolines with high regio- and diastereoselectivity.
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  • 76
    ISSN: 1434-193X
    Keywords: Asymmetric synthesis ; Zinc ; Transition structures ; QM/MM computations ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Trimethylsilyl substitutions of the fenchyl alcohols [(1R,2R,4S)-exo-(2-Ar)-1,3,3-trimethylbicyclo[2.2.1]heptan-2-ol, Ar = 2-methoxyphenyl (1) and Ar = 2-(dimethylaminomethyl)phenyl (2)] yield the chiral ligands 3 [Ar = 2-methoxy-3-(trimethylsilyl)phenyl] and 4 [Ar = 2-(dimethylaminomethyl)3-(trimethylsilyl)phenyl]. Increased reactivities and enantioselectivities in diethylzinc additions to benzaldehyde are obtained from 3 (63% ee R) and 4 (93% ee S), relative to 1 (26% ee S) and 2 (73% ee S). X-ray crystal structures of 3 and of its methylzinc complex 3-Zn reveal out-of-plane bending of the methoxy groups as major geometrical consequences of the trimethylsilyl substitutions. Analyses of QM/MM ONIOM μ-O transition-structure models for 1, 2, 3, and 4 show that trimethylsilyl-induced distortions of methoxy and of dimethylaminomethyl groups explain the observed increased enantioselectivities.
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  • 77
    ISSN: 1434-193X
    Keywords: Palladium ; exo-π-Allylpalladium complexes ; Allylic alkylation ; Spiro compounds ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The palladium(0)-catalyzed alkylation of 2,3-bis(acetoxymethyl)bicyclo[2.2.1]hepta-2,5-diene 1 with malonate-type enolates as nucleophiles is investigated. A monoalkylated product is formed first, and undergoes (depending on the nucleophile used) a second intramolecular reaction leading to spirocyclopropane-annulated bicyclo[2.2.1]heptene derivatives 5. The formation of endo spirobicyclic cyclopropanes adducts as major isomer is rationalized by assuming formation of an intermediate exo-(π-allyl)palladium complex.
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  • 78
    ISSN: 1434-193X
    Keywords: Ketones ; Reductions ; Micelles ; Cobalt ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Operationally simple and environmentally benign procedures have been developed to selectively reduce different α,β-unsaturated ketones, 4,4-dimethylcyclohex-2-ene-1-one (1), isophorone (2), benzylideneacetone (3), chalcone (4) by NaBH4 or by the system NaBH4 + CoCl2. Alternative reaction media to the extensively used MeOH have been explored, and new procedures take advantage of the acceleration and chemoselectivity induced by water or by aqueous micellar solutions. It was possible to selectively and quantitatively afford pure products of 1,2 and of 1,4 reduction as well as the totally reduced compounds (yield and selectivity 〉 90%) by simple changes in the experimental conditions.
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  • 79
    ISSN: 1434-193X
    Keywords: C-Glycosides ; Conformation analysis ; Molecular dynamics ; Selectins ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The conformational behavior of the C-glycosyl analogue of sialyl-α-(2→3)-galactose, synthesized as a glycosidase inhibitor, has been studied using a combination of NMR spectroscopy (J and NOE data) and molecular dynamics calculations. The obtained results show that the population distribution of conformers with respect to the orientation about the pseudo-glycosidic linkages is mainly controlled by steric interactions. This is in contrast to findings made for O-glycosides. In these natural compounds, the conformational behavior about the glycosidic linkage Φ is mainly governed by the exo-anomeric effect.
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  • 80
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    Liebigs Annalen 2000 (2000), S. 1815-1820 
    ISSN: 1434-193X
    Keywords: Asymmetric synthesis ; Palladium-catalysed couplings ; Palladacycles ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Chiral C2-symmetric N-benzylazetidines have been conveniently prepared from optically pure anti-1,3-diols without loss of enantiomeric purity. N-Debenzylation led to the corresponding N-unsubstituted azetidines, which were then subjected to palladium-catalysed coupling reactions with aryl bromides to afford chiral N-arylazetidines. (R,R)-N-Benzyl-2,4-dimethylazetidine has been employed in the synthesis of a new cyclopalladated complex, which can be used, for instance, as a chiral recognition agent for phosphorus ligands.
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  • 81
    ISSN: 1434-193X
    Keywords: Cyclizations ; Spiro compounds ; Nucleosides ; Isomerizations ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Syntheses of the spiro nucleosides 2′-deoxyhydantocidin 3a and its 1′-epimer 3b are described. The newly developed route involves a Horner-Wadsworth-Emmons condensation of the phosphonate 16 with the erythrose derivative 15 affording a mixture of six isomers which was fully assigned by NMR spectroscopy. The mixture was directly converted into the final compounds in an efficient base-catalyzed cyclization reaction. A base-catalyzed interconversion between the two isomers was observed.
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  • 82
    ISSN: 1434-193X
    Keywords: Benzodiazepines ; Heterocycles ; Azeto[1,2-a]-1,5-benzodiazepines ; Cyclizations ; Cycloadditions ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The reaction of o-phenylenediamine (4) with one, two or three equivalents of p-substituted 3-dimethylaminopropiophenone hydrochlorides 5a-e was studied. 4-Aryl-2,3-dihydro-1H-1,5-benzodiazepine derivatives 6a-e were obtained in good yields, along with the 1:2-adducts 7c-e and the unexpected 1:3-adducts rac-8c-e. The type of adduct formed is determined by the molar ratio of the reactants 4 and 5 and by the nature of the substituent in the para position of the propiophenone 5.
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  • 83
    ISSN: 1434-193X
    Keywords: Cup-shaped PAHs ; Host-guest compounds ; Host-guest chemistry ; Cyclizations ; Semiempirical calculations ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Cyclotrimerisation of benzo-polycyclic bromostannylalkenes 8a-d with Cu(NO3)2·3H2O in THF affords benzotri(benzonorbornadienes) 3a-d as a mixture of the syn and anti isomers. The ratio of syn to anti is close to the 1:3 statistical value in most cases (i.e. in cyclotrimers 3a,b,d), but highly in favour of the anti isomer in 3c, where steric hindrance by the methoxy groups plays an important role in the stereochemistry of the cyclotrimerisation. The substrates for the cyclotrimerisation, i.e. the bromostannyl alkenes 8a-d, were prepared from bromoalkenes 7a-d by treatment with base (LDA) and quenching with trimethyltin chloride. In turn, bromoalkenes 7a-d were prepared from alkenes 5a-d by radical bromination-elimination. The reaction conditions used were designed to minimise Wagner-Meerwein rearrangements that would lead to unwanted bromo isomers. The cup-shaped syn cyclotrimers 3a-d exhibit high electron density within the cavity as determined by AM1 semiempirical calculations of their electrostatic potential surfaces and are valuable substrates for supramolecular chemistry. As an example, it is shown that fullerene C60 is drawn into solution in acetonitrile by complexation with both the syn and anti trimer of benzonorbornadiene 3a.
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  • 84
    ISSN: 1434-193X
    Keywords: Carbene complexes ; Enamines ; Metallatrienes ; Cyclopentadienes ; Dihydropyrroles ; spiro-Tetrahydropyrroles ; Iminium carbonylmetalates ; Dimetallapolyenes ; Tungsten ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Reactions of the [(1-alkynyl)carbene]tungsten complex (CO)5W=C(OEt)C≡CPh (1) with 1-aminocyclohexenes 2a-c and 7a-c afford different types of products depending on the amino substituents and the reaction conditions. (4-Aminocyclobutenyl)carbene complexes B have been shown to be generated in the first reaction step through a [2+2] cycloaddition. These are key intermediates and afford cross-conjugated tungstatrienes E, (conjugated) 1-tungsta-1,3,5-hexatrienes G, or (non-conjugated) 1-tungsta-1,3,6-heptatrienes F by following competing reaction pathways. Cross-conjugated 1-tungstatrienes 3 have been isolated in 52-74% yield by performing the reactions of 1-aminobenzocyclohexenes 2a-c with compound 1 in pentane. In dichloromethane instead of pentane, (conjugated) 1-tungsta-1,3,5-hexatrienes 4 are obtained, which subse-quently undergo fragmentation to give cyclopentadienes 6 (by π-cyclization) and dihydropyrroles (by α-cyclization) in a molar ratio dependent on the nature of the amino substituents. (Non-conjugated) 1-tungsta-1,3,6-heptatrienes 10 are generated upon reaction of 1-aminocyclohexenes 7a-c with compounds 1, which are transformed into cyclopentadienes 12 via conjugated 1-tungsta-1,3,5-hexatrienes 9 as intermediates. Reactions of 1-tungsta-1,3,6-heptatrienes 10 with the (1-alkynyl)carbene complex 1 afford dinuclear compounds 14, which subsequently yield indenes 15 (by two successive π-cyclization steps) and spiro-tetrahydropyrroles 16 (by both a π-cyclization and an α-cyclization step), depending on the steric bulk of the amino substituent.
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  • 85
    ISSN: 1434-193X
    Keywords: Allyl alcohols ; Organocerium reagents ; Alkenes ; Addition reactions ; Cerium ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Alkylcerium reagents add to the multiple bonds of allyl and propargyl alcohols in good yields and under mild conditions. The double bond can be reduced with lithium aluminum hydride in the presence of cerium trichloride. The regiochemistry of the attack depends on electronic factors.
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  • 86
    ISSN: 1434-193X
    Keywords: Amidines ; Chiral bases ; N-Acyliminium ions ; Enantioselective catalysis ; Chiral pool ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The synthesis of four enantiopure hydroxyamidines is described. One amidine was obtained from (S)-pyroglutamic acid. Its key step involved the addition of phenylmagnesium bromide to the corresponding ester, affording the tertiary alcohol without detectable racemization. The second amidine was obtained by coupling of an (S)-malic acid derived N-acyliminium ion with β-naphthol. The other amidines were obtained from an (S)-serine-derived imide which was reduced to two diastereomeric lactams that were eventually transformed into the corresponding amidines.
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  • 87
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 2000 (2000), S. 155-163 
    ISSN: 1434-193X
    Keywords: Macrocycles ; Cyclizations ; Strained compounds ; Ketophosphonate ; Iodoalkyne ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Two strategies have been explored to build the highly strained eleven-membered ring 2, a potential precursor for the biosynthetic key intermediate of the protoilludane family: an intramolecular Horner-Wadsworth-Emmons olefination and an intramolecular Nozaki-Hiyama-Kishi type-ring closure.
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  • 88
    ISSN: 1434-193X
    Keywords: Neutral anion receptor ; Hydrogen bonds ; Urea moieties ; Donor-acceptor systems ; Macrocycles ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The synthesis of macrocyclic and acyclic cleft-like anion receptors in which four hydrogen bond donating urea moieties are present in a preorganized fashion is described. NMR spectroscopy shows the complex formation with H2PO4- and Cl -. Cleft-like receptors bind H2PO4- in a 2:1 guest-host stoichiometry (Ka = 107M-2) in DMSO, whereas Cl - is bound in a 1:1 stoichiometry (Ka = 103M-1). The macrocyclic receptors form a 1:1 complex with H2PO4- (Ka = 103M-1 in DMSO) with a 100-fold selectivity for H2PO4- over Cl -.
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  • 89
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 2000 (2000), S. 193-198 
    ISSN: 1434-193X
    Keywords: Nucleophilic substitutions ; Azulenes ; Hydroxylation ; Amination ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Hydroxylation of azulenes with tert-butylhydroperoxide proceeds efficiently at the 6-position when the former contain electron-withdrawing substituents in the five-membered ring. Similarly, VNS amination of azulenes proceeds with 4-amino-1,2,4-triazole; its anion, being an active nucleophile, also reacts with unsubstituted azulene. A variety of transformations of 6-hydroxyazulenes, such as substitution of the corresponding sulfonates with nitrogen, oxygen, sulfur, carbon nucleophiles and halogens, and the Claisen rearrangement of allylic ethers, is reported.
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  • 90
    ISSN: 1434-193X
    Keywords: Heterocycles ; Carbohydrates ; Imidazolidines ; Oxazolidines ; Spiro compounds ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Prochiral 1,3-dihydroxyacetone forms racemic oxazolidine- and oxazoline-type spiro[4.4]nonanes upon reactions with potassium (thio)cyanate and cyanamide. In contrast, 1,3-diaminoacetone yields only the corresponding spiro-bisimidazolidinethione under similar conditions together with monocyclic by-products, but the spiro-bisimidazolidinone is accessible by reaction of 1,3-dichloroacetone with urea. The resolution of the racemic spiro-bisoxazolidinethione 2a was achieved by using brucine as the resolving agent.
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  • 91
    ISSN: 1434-193X
    Keywords: Homogeneous catalysis ; Palladium ; Acetoxylation ; Acryloxylation ; Addition reactions ; Cyclooctadiene ; Cyclooct-4-enone ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Palladium-catalyzed acryloxylation of several cycloalkenes in the presence of the Pd(OAc)2/p-benzoquinone/MnO2 catalytic system is reported. This oxidation reaction yields allylic acrylates as the sole products through the intermediacy of an η3-allyl complex. However, with 1,5-cyclooctadiene (3), cyclooct-4-enone (4) is the major reaction product. Its formation results from oxidation, which is also observed in the palladium-catalyzed acetoxylation of 3. It has been shown that this is the result of a 1,2-trans addition (acryloxy-palladation), followed by a β-hydride elimination, which yields an enol acrylate or acetate. A second Markovnikov-oriented 1,2-trans-acryloxy-palladation on the latter yields a palladium complex, rearrangement of which gives 4.
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  • 92
    ISSN: 1434-193X
    Keywords: Amino acids ; coupling ; N-Substituted amide ; Cyclizations ; Cyclic dipeptides ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---A short synthesis of new, functionalized seven-membered ring cyclic dipeptides is described. After the coupling of N-protected β-amino acids to N-substituted α-amino tert-butyl esters, the protective groups of the terminal functions were removed and the cyclization took place diastereoselectively in the presence of the coupling agent BOP. Amide substitution was found to be effective in promoting the cyclization of linear dipeptides.
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  • 93
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 2000 (2000), S. 275-280 
    ISSN: 1434-193X
    Keywords: Radical reactions ; Substituent effects ; Nitrogen heterocycles ; Dihydropyridines ; Pyrrolines ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---A set of allene-tethered benzoyloximes (5) has been treated with nBu3SnH. Depending on their substitution pattern, a wide range of compounds has been obtained. If the stannyl radical adds on the allene, the C-centred radical thus formed undergoes either a 5-exo ring closure to give the cyclopentene derivatives 7 or a 6-endo ring closure onto the N atom to give the dihydropyridines 8. If the stannyl radical adds on the benzoyl moiety, an iminyl radical is formed which leads to the 3H-pyrroles 9 and the alkylidene-pyrrolines 10. Steric effects as well as polar effects are the factors governing the reaction course.Supporting information for this article is available on the WWW under //http://www.wiley-vch.de/contents/jc_2046/2000/99373_s.pdf or from the author.
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  • 94
    ISSN: 1434-193X
    Keywords: Antibiotics ; Configuration determination ; Heterocycles ; Hongoquercin A ; Terpenoids ; Total synthesis ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---(±)-Hongoquercin A (1), the racemate of an antibacterial fungal metabolite, has been synthesized starting from geranylacetone (2) and ethyl orsellinate (ethyl 2,4-dihydroxy-6-methylbenzoate, 5). The structure (±)-1 has been confirmed by X-ray analysis of its ethyl ester (±)-10. Synthesis of the naturally occurring (+)-hongoquercin A from (-)-sclareol (11) established its configuration as depicted in 1.
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  • 95
    ISSN: 1434-193X
    Keywords: Carbohydrates ; Amino sugars ; Protecting groups ; Glycosylations ; Trichloroacetimidates ; Oligosaccharides ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The N-DMM-Protected lactosamine derivative 2 was readily transformed into the corresponding glycosyl donor 4 and into acceptor 5. A TMSOTf-catalyzed glycosidation afforded the derived tetrasaccharide 6 which led to glycosyl donor 9. Reaction of 9 with lactose derivative 10 as acceptor gave the desired hexasaccharide 11. Cleavage of all protective groups and N-acetylation afforded the target molecule 1b (lacto-N-neohexaose). Glycosylation of acceptor 10 with donor 4 furnished tetrasaccharide 16 which, employing standard procedures, gave acceptor 18. Glycosylation of 18 with donor 9 furnished, under standard conditions, octasaccharide 19. Cleavage of all protective groups and N-acetylation afforded the target molecule 1c (lacto-N-neooctaose). Both 1b and 1c were obtained in good overall yields.
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  • 96
    ISSN: 1434-193X
    Keywords: Sharpless asymmetric dihydroxylation ; Asimilobin ; Tetrahydrofuran ring ; Wittig reactions ; Annonaceous acetogenins ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The most efficient method for the synthesis of the trans/threo/trans-bis(tetrahydrofuran) (THF) ring unit was established, and the first total synthesis of (-)-asimilobin and its diastereomer was then accomplished in twelve and fourteen steps, respectively, from trans-1,5,9-decatriene, by a convergent route with a Wittig reaction as the key step. By virtue of these synthetic results, the absolute configuration of the bis(THF) unit in naturally occurring asimilobin should be corrected.
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  • 97
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 2000 (2000), S. 381-385 
    ISSN: 1434-193X
    Keywords: Enynes ; Macrocycles ; Coordination chemistry ; Crown compounds ; Radicals ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The complex molecular structure and interesting activation mechanisms of naturally occurring enediynes have inspired the synthesis of a variety of simpler model systems to mimic their properties. While in most model compounds nucleophilic attack, isomerization or bioreduction are used to activate the unsaturated system for diradical cyclization, some attempts have been made to employ metal-ion coordination for this purpose. Significant enhancement of the thermal reactivity has been achieved by metal-ion induced conformational and electronic changes of suitably substituted enediynes, such as 1, 5, 6, 17 and 18. Enediyne activation by stoichiometric or catalytic formation of vinylidene complexes, such as 22, from terminal alkynes has also been investigated. This paper summarizes recent results pursuing the activation of enediyne diradical cyclization by metal ions.
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  • 98
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 2000 (2000), S. 419-423 
    ISSN: 1434-193X
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The first enantioselective synthesis of enantiomerically pure (-)-wine lactone, (-)-1a, a fragrance constituent of various white wines, and its epimer (+)-1b, was carried out. The key steps are allylic substitution of (±)-2-cyclohexen-1-yl acetate (2) with dimethylmalonate using palladium complexes of phosphanyldihydrooxazol L1 or of the phosphanylcarboxylic acid L2 as catalyst, subsequent decarboxylation, iodolactonization and elimination, furnishing enantiomerically pure bicyclic lactone (+)-7 in 47% overall yield. The diastereoselective introduction of methyl groups by SN2′-type substitution with an organocopper compound and by enolate alkylation gave lactone (-)-1a in 43% overall yield from (+)-7.
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  • 99
    ISSN: 1434-193X
    Keywords: Aplykurodins ; Marine steroids ; C-C coupling ; Cyclic peroxides ; Lactonization ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---An approach to the synthesis of aplykurodins, ichthyotoxic marine lactones, is presented. The carbon framework was derived from vitamin D3 by conversion of the readily accessible allyl alcohol 13 to the protected Grundmann's hydroxy ketone 22 and subsequent introduction of the C2 side chain through a Pd0-promoted coupling. Highly stereoselective hetero Diels-Alder reaction with O21 produced the key intermediate peroxide 25. Functional group transformations, coupled with a series of chemo- and stereoselective reactions, finally resulted in the synthesis of the unnatural analogue 17,18-dihydro-3,9-di-epi-aplykurodinone B (6).
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  • 100
    ISSN: 1434-193X
    Keywords: Zeolites ; Radical ions ; Ring opening ; Electron transfer ; Oxidations ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Incorporation of trans-1,2-diphenylcyclopropane (1) and its 3,3-D2-isotopomer into the channels of a redox-active pentasil zeolite (Na-ZSM-5) resulted in the formation of exo,exo-1,3-diphenylallyl radical (2•) and its 2-D1 isotopomer, respectively. This conversion requires oxidation, ring opening, and deprotonation, in an unspecified sequence. The allyl radical 2• is also formed upon incorporation of trans-1,3-diphenylpropene (3). A comparison with the solution photochemistry, in the presence or absence of added base, shows the conversion of 1 into 2• to be a zeolite-specific reaction. Incorporation of arylcyclopropanes 9 (R = H, OCH3) into ZSM-5 generated trans-propenylbenzene radical cations 10•+ (R = H, OCH3); the 2,2-D2 isotopomer of 9 (R = OCH3) gave rise to three different isotopomers of 10•+ (R = OCH3).
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