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  • Other Sources  (32)
  • Cell & Developmental Biology
  • Deutschland
  • Life Sciences (General)
  • 1975-1979  (27)
  • 1965-1969  (5)
  • 1
    facet.materialart.
    Unknown
    In:  Bundesgesundheitsblatt 22, 8-13
    Publication Date: 1979
    Description: einheimische Malaria als man-made-disease KATASTER-BESCHREIBUNG: KATASTER-DETAIL:
    Keywords: Deutschland ; Umweltmedizin ; Infektionskrankheiten
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  • 2
    Publication Date: 2011-08-24
    Description: The third in a series of Spacelab Mission Development tests was conducted at the Johnson (correction of Johnston) Space Center as a part of the development of Life Sciences experiments for the Space Shuttle era. The latest test was a joint effort of the Ames Research and Johnson Space Centers and utilized animals and men for study. The basic objective of this test was to evaluate the operational concepts planned for the Space Shuttle life science payloads program. A three-man crew (Mission Specialist and two Payload Specialists) conducted 26 experiments and 12 operational tests, which were selected for this 7-day mission simulation. The crew lived on board a simulated Orbiter/Spacelab mockup 24 hr a day. The Orbiter section contained the mid deck crew quarters area, complete with sleeping, galley and waste management provisions. The Spacelab was identical in geometry to the European Space Agency Spacelab design, complete with removable rack sections and stowage provisions. Communications between the crewmen and support personnel were configured and controlled as currently planned for operational shuttle flights. For this test a Science Operations Remote Center was manned at the Ames Research Center and was managed by simulated Mission Control and Payload Operation Control Centers at the Johnson Space Center. This paper presents the test objectives, description of the facilities and test program, and the results of this test.
    Keywords: Life Sciences (General)
    Type: Acta astronautica (ISSN 0094-5765); Volume 6; 10; 1239-49
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  • 3
    Publication Date: 2013-08-31
    Description: At the age of 30 days female Sprague-Dawley rats were placed on a 3.66 m radius centrifuge and subsequently exposed almost continuously for 810 days to either 2.76 or 4.15 G. An age-matched control group of rats was raised near the centrifuge facility at earth gravity. Three further control groups of rats were obtained from the animal colony and sacrificed at the age of 34, 72 and 102 days. A total of 16 variables were simultaneously factor analyzed by maximum-likelihood extraction routine and the factor loadings presented after-rotation to simple structure by a varimax rotation routine. The variables include the G-load, age, body mass, femoral length and cross-sectional area, inner and outer radii, density and strength at the mid-length of the femur, dry weight of gluteus medius, semimenbranosus and triceps surae muscles. Factor analyses on A) all controls, B) all controls and the 2.76 G group, and C) all controls and centrifuged animals, produced highly similar loading structures of three common factors which accounted for 74%, 68% and 68%. respectively, of the total variance. The 3 factors were interpreted as: 1. An age and size factor which stimulates the growth in length and diameter and increases the density and strength of the femur. This factor is positively correlated with G-load but is also active in the control animals living at earth gravity. 2. A growth inhibition factor which acts on body size, femoral length and on both the outer and inner radius at mid-length of the femur. This factor is intensified by centrifugation.
    Keywords: Life Sciences (General)
    Type: Anatomy and Embryology (ISSN 0340-2061); Volume 156; 89-101
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  • 4
    Publication Date: 2019-07-17
    Description: Many pharmacological studies have implicated the biogenic amines in the hypothalamus as playing a role in the production of fever, but few investigations of endogenous neurochemicals have been made during fever. Turnover rates of transmitters utilizing radioactive precursors may be one of the most accurate measurements of activity in brain regions. The present study was designed to measure the turnover of 5-hydroxytryptamine (5-HT) norepinephrine (NE) and dopamine (DA) in the hypothalamus of rats during pyrogen fever. Salmonella typhosa (Wyeth, 8 units) was previously found in our laboratory to produce a significant hyperthermia in most rats by 2.5 hours. This pyrogen (N = l2) or saline control (N = 8) was injected intraperitoneally and the rats killed 2.75 hours later. Rectal temperatures (Tr) were monitored continuously with thermocouples taped to the tail and recorded automatically every 3 minutes. Half of each group received an injection of radioactive precursors, (3)H-tryptophan (0.5 mCi) and (3)H-tryptophan (1.0 mCi), via an indwelling jugular catheter 60 minutes before killing, and the other half at 90 minutes. The rats were killed by near freezing in liquid nitrogen and the brains dissected in the cold. Turnover was measured by the method of Lane (Life Sci 21, 1101, 1977). At the time of killing most of the pyrogen group showed a significant (p 〈 .02) increase (mean +/- s.e.m.) in Tr above pre injection levels (0.75 +/- 0.13 C, N = 10). The saline group showed no change (-0.025 +/- 0.16, N = 8), and the difference between groups was also significant. No significant differences were found in the levels of the amines between the pyrogen and saline groups. A significant difference was found in the specific activity of NE between the 60 minute pyrogen and saline groups (4.41 +/- 0.41 vs 2.6 +/- 0.51 dpm/pmole) but no change in turnover. This suggests an increased accumulation of (3)H-NE in the pyrogen group, but no change in utilization. An increased turnover of DA for the pyrogen group (44.5 vs 19.2 pmole/mg protein/hr) was found. However, DA is mainly a precursor in the hypothalamus and measurement was near the limit of sensitivity for the assay; these limitations Must be considered in interpreting this data. The most significant finding was an increase in the turnover of 5-HT in the pyrogen group (41.3 vs 7.3 pmole/mg protein/hr), indicating a resynthetization rate of 77% of the total pool per hour. These results suggest that at the time point measured, an increase in the utilization of 5-HT in the hypothalamus is-correlated with pyrogen fever.
    Keywords: Life Sciences (General)
    Type: Society for Neuroscience Abstracts; 3
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  • 5
    Publication Date: 2019-07-13
    Description: Chronic centrifugation of 85- to 92-day-old Beagles at 2.0 x g and 2.6 x g for 26 weeks during the time of active skeletal growth caused skeletal abnormalities in the radius and the ulna of ten of 11 dogs. The pattern of change mimicked that found in naturally occurring and experimentally induced premature distal ulnar physeal closure or delayed growth at this physis. Minimal changes in bone density were detected by sensitive photon absorptiometric techniques. Skeletal abnormalities also were found in five of the six cage-control dogs, although the run-control dogs were radiographically normal.
    Keywords: Life Sciences (General)
    Type: American Journal of Veterinary Research; 40; 3; 346-350
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  • 6
    Publication Date: 2019-07-13
    Description: The environmental factors which affect humans and other animals also influence the microorganisms which are such an important part of our ecology. Some of the microorganisms are very closely associated with animals, living in the digestive tract and synthesizing essential nutrients for the host. For these microbes, most external physical changes are of little consequence, because they are well shielded by the animals' homeostatic systems. The vast majority of microorganisms, however, live free in nature, especially in the soil and oceans. It has been estimated that the upper 15 cm of a fertile soil may contain over 4000 kg of bacteria and fungi per hectare. These organisms are responsible for degrading the complex molecules of plants and animals when they die, eventually producing simple organics, carbon dioxide, and inorganics, which are then used for the next cycle of plant growth. It is believed that over 90 % of the biologically produced carbon dioxide results from the metabolic activity of bacteria and fungi. In addition to recycling plant nutrients, soil bacteria also provide new nutrients through 'fixation' of atmospheric nitrogen into ammonia and nitrate, the forms which can be used by plants. Microorganisms so have an enormous capacity for detoxifying both natural and man-made poisons. All of these functions of microorganisms are essential to the operation of the material cycles on Earth. This is true of all locations on the planet, regardless of the climate or other environmental factors. In fact, one of the most impressive attributes of microorganisms is their ability to adapt to every stable environment on Earth. These include such extremes as polar regions, hot springs, water saturated with salt, mountain tops, ocean depths, acid and alkaline waters, deserts, intense radioactivity, soil and water contaminated with toxic chemicals or petroleum, and areas devoid of oxygen.
    Keywords: Life Sciences (General)
    Type: NASA/TM-1979-207891 , NAS 1.15:207891 , Biometeorological Survey, Part A: Human Biometeology; 1; 162-169
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  • 7
    Publication Date: 2019-07-13
    Description: A mechanism whereby sound is generated by the motion of vortices in the human lung is described. This mechanism is believed to be responsible for most of the sound which is generated both on inspiration and expiration in normal lungs. Mathematical expressions for the frequencies of sound generated, which depend only upon the axial flow velocity and diameters of the bronchi, are derived. This theory allows the location within the bronchial tree from which particular sounds emanate to be determined. Redistribution of pulmonary blood volume following transition from Earth gravity to the weightless state probably alters the caliber of certain airways and doubtless alters sound transmission properties of the lung. We believe that these changes can be monitored effectively and non-invasively by spectral analysis of pulmonary sound.
    Keywords: Life Sciences (General)
    Type: Acta astronautica (ISSN 0094-5765); 6; 9; 1137-51
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  • 8
    Publication Date: 2019-07-13
    Description: Conditioned aversions to colored, flavored water were established in Squirrel monkeys (Saimiri sciureus) by following consumption with 90 min of simultaneous rotational and vertical stimulation. The experimental group (N= 13) drank significantly less of the green, almond-flavored test solution than did the control group (N=14) during three post-treatment preference testing days. Individual differences were noted in that two experimental monkeys readily drank the test solution after rotational stimulation. Only two of the experimental monkeys showed emesis during rotation, yet 10 monkeys in this group developed an aversion. These results suggest that: (1) motion sickness can be readily induced in Squirrel monkeys with simultaneous rotational and vertical stimulation, and (2) that conditioned food aversions are achieved in the absence of emesis in this species.
    Keywords: Life Sciences (General)
    Type: NASA-CR-203358 , NAS 1.26:203358 , Physiology & Behavior (ISSN 0031-9384); 23; 39-41
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  • 9
    Publication Date: 2019-08-17
    Description: Small, round, bio-compatible microspheres capable of covalently bonding proteins and having a uniform diameter below about 3500 A are prepared by substantially instantaneously initiating polymerization of an aqueous emulsion containing no more than 35% total monomer including an acrylic monomer substituted with a covalently bondable group such a hydroxyl, amino or carboxyl and a minor amount of a cross-linking agent.
    Keywords: Life Sciences (General)
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  • 10
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    Unknown
    In:  Kartoffelbau 29 (1):15-17.
    Publication Date: 1978
    Description: Zusammenhang Kartoffelertrag und -qualität und Witterungsverlauf KATASTER-BESCHREIBUNG: Einfluss der Witterung (Temperatur, Niederschlag) auf den Ernteertrag KATASTER-DETAIL: Delta T (Mai, Juni) + und Delta Nied (Mai, Juni) -, dann Pflanzenentwicklung -; Delta Bodenfeuchte -, dann Wachstum -; Delta T (März, April) 〈 0°C, dann Schäden +;
    Keywords: Deutschland ; 1975-77 ; Kartoffeln ; Ertrag ; Landwirtschaft ; Niederschlag ; Temperatur ; Witterung
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  • 11
    facet.materialart.
    Unknown
    In:  Other Sources
    Publication Date: 2011-08-23
    Description: The three experimental approaches incorporated into the Viking biology instrument have yielded results that are most readily explained as nonbiological phenomena. The predominant view among investigators trying to simulate the Mars results is that the surface material of Mars contains strongly oxidizing compounds which would account for many of the more intense reactions seen on Mars. Other mechanisms are also currently being proposed and studied.
    Keywords: Life Sciences (General)
    Type: Origins of Life; Volume 9; 157-160
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  • 12
    Publication Date: 2019-07-17
    Description: The activities of about 30 enzymes concerned with carbohydrate and lipid metabolism and the levels of glycogen and of individual fatty acids were measured in livers of rats ex- posed to prolonged space flight (18.5 days) aboard COSMOS 986 Biosatellite. When flight stationary, (FS) and flight centrifuged (FC) rats were compared at recovery (R(sub 0)), decrceases in the activities of glycogen phosphorylase, alpha glycerphosphate, acyl transferase, diglyceride acyl transferase, acconitase and Epsilon-phosphogluconate dehydrogenase were noted in the weightless group (FS). The significance of these findings was strengthened since all activities, showing alterations at R(sub 0), returned to normal 25 days post-flight. Differences were also seen in levels of two liver constituents. When glycogen and total fatty acids of the two groups of flight animals were determined, differences that could be attributed to reduced gravity were observed, the FS group at R(sub 0) contained, on the average, more than twice the amount of glycogen than did controls ad a remarkable shift in the ratio of palmitate to palmitoleate were noted. These metabolic alterations appear to be unique to the weightless condition. Our data justify the conclusion that centrifugation during space flight is equivalent to terrestrial gravity.
    Keywords: Life Sciences (General)
    Type: Physiologist; 21; 4
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  • 13
    Publication Date: 2019-07-17
    Description: Although cumulative effects an retina from low-dose radiation during prolonged spaceflight are not known, ary impairment of vision could set limits for spaceflight duration. Cosmic rays are now considered to be the cause of the "light flashes" seen during spaceflight by activating retina cells as they pass through the photoreceptors. Previous studies have also shown retinal cellular alterations and cell necrosis from high-energy, particle (HZE) radiation. Ten rats, 5 centrifuged during flight (FC) to simulate gravity and 5 in-flight stationary (FS) experiencing hypogravity, orbited Earth for 18.5 days on Cosmos 936. The animals were sacrificed 25 days post-recovery and the eyes flown to Ames Res. Ctr. The pattern of cell necrosis in the retinas from the FC group showed the same response to radiation as the FS. This would indicate that hypogravity was not a factor in the observed results. Also the cellular response in the retinas exposed in the Berkeley accelerator again matched both the FC and FS eyes. Thus all three conditions provide comparable changes and indicate HZE particles as the possible cause of the cellular alterations, channels, and breakdown.
    Keywords: Life Sciences (General)
    Type: Physiologist; 21; 4
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  • 14
    Publication Date: 2011-08-24
    Description: Until recently, human +Gz acceleration tolerance has relied solely on subjective criteria relating to loss of vision. By use of newly developed noninvasive instrumentation using a transcutaneous Doppler flow system, objective end point criteria have been developed based on measured blood flow to the head. The system consists of miniature 8 MHz Doppler sensors (2 x 1 x 0.5 cm) placed on the forehead over both frontal branches of the temporal arteries to detect blood flow velocity from back scattered ultrasound. Its use has allowed for correlation of altered, decreased and actual reversal of eye level blood flow with subsequent central light loss. Over 100 subjects have now been studied during more than 2,000 centrifuge runs. Objective changes in temporal artery flow velocity consistently preceded visual degradation for each subject during all acceleration profiles. No subject has gone unconscious without first exhibiting a minimum 6 sec of total flow cessation. Retrograde flow followed by complete flow cessation always preceded central light loss. Results indicate that this method can be successfully used with a wide variety of tasks during exposure to +Gz acceleration. It is recommended for use during evaluation of protective maneuvers or devices on the centrifuge or during actual flight in high performance aircraft. It may also serve as a potential safety monitor during space Shuttle re-entry if there is doubt about a passenger's cardiovascular status.
    Keywords: Life Sciences (General)
    Type: Acta astronautica (ISSN 0094-5765); Volume 4; 5-6; 541-53
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  • 15
    Publication Date: 2011-08-23
    Description: Cell envelope vesicles prepared from H. halobium contain bacteriorhodopsin and upon illumination protons are ejected. Coupled to the proton motive force is the efflux of Na(+). Measurements of Na-22 flux, exterior pH change, and membrane potential, Delta(psi) (with the dye 3,3'-dipentyloxadicarbocyanine) indicate that the means of Na(+) transport is sodium/proton exchange. The kinetics of the pH changes and other evidence suggests that the antiport is electrogenic (H(+)/Na(++ greater than 1). The resulting large chemical gradient for Na(+) (outside much greater than inside), as well as the membrane potential, will drive the transport of 18 amino acids. The I9th, glutamate, is unique in that its accumulation is indifferent to Delta(psi): this amino acid is transported only when a chemical gradient for Na(+) is present. Thus, when more and more NaCl is included in the vesicles glutamate transport proceeds with longer and longer lags. After illumination the gradient of H+() collapses within 1 min, while the large Na(+) gradient and glutamate transporting activity persists for 10- 15 min, indicating that proton motive force is not necessary for transport. A chemical gradient of Na(+), arranged by suspending vesicles loaded with KCl in NaCl, drives glutamate transport in the dark without other sources of energy, with V(sub max) and K(sub m) comparable to light-induced transport. These and other lines of evidence suggest that the transport of glutamate is facilitated by symport with Na(+), in an electrically neutral fashion, so that only the chemical component of the Na(+) gradient is a driving force.
    Keywords: Life Sciences (General)
    Type: Journal of Supramolecular Structure; Volume 6; 169-177
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  • 16
    Publication Date: 2011-08-24
    Description: The light-intensity threshold for humans is not known. In past space flights owing to power restrictions, light intensities have been minimal and reported to be as low as 15 ft. c. This study was conducted to determine whether the light (L)/dark (D) environment of 16L : 8D at the relatively low light intensity of 15 ft. c. was adequate for the maintenance of circadian synchrony in human subjects. Six healthy male subjects aged 20-23 years were exposed for 21 days to a 16L : 8D photoperiod. During the first 7 days the light intensity was 100 ft. c.; it was reduced to 15 ft. c. during the next 7 days and increased again to 100 ft. c. during the last 7 days of the study. Rectal temperature (RT) and heart rate (HR) were recorded continuously throughout the 21 days of the study. In the 100 ft. c. 16L : 8D the RT and HR rhythms remained stable and circadian throughout. When the light intensity was decreased to 15 ft. c. the periodicity of the HR rhythm was significantly decreased and this rhythm showed marked instability. In contrast the period of the RT rhythm did not change but a consistent phase delay occurred due to a delay in the lights-on associated rise in RT. These divergent effects on these two rhythms in internal desynchronization and performance decrement during the 15 ft. c. exposure. The data emphasize the need for establishing accurately the minimal lighting requirements for the maintenance of circadian rhythms of humans in confined environments.
    Keywords: Life Sciences (General)
    Type: Life sciences and space research (ISSN 0075-9422); Volume 15; 233-7
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  • 17
    Publication Date: 2019-07-13
    Description: The accumulation of 20 commonly occurring L-amino acids by cell envelope vesicles of Halobacterium halobium, in response to light-induced membrane potential and an artificially created sodium gradient, has been studied. Nineteen of these amino acids are actively accumulated under either or both of these conditions. Glutamate is unique in that its uptake is driven only by a chemical gradient for sodium. Amino acid concentrations at half-maximal uptake rates (Km) and maximal transport rates (V(sub max) have been determined for the uptake of all 19 amino acids. The transport systems have been partially characterized with respect to groups of amino acids transported by common carriers, cation effects, and relative response to the electrical and chemical components of the sodium gradient, the driving forces for uptake. The data presented clearly show that the carrier systems, which are responsible for uptake of individual amino acids, are as variable in their properties as those found in other organisms, i. e., some are highly specific for individual amino acids, some transport several amino acids competitively, some are activated by a chemical gradient of sodium only, and some function also in the complete absence of such a gradient. For all amino acids, Na(+) and K(+) are both required for maximal rate of uptake. The carriers for L-leucine and L-histidine are symmetrical in that these amino acids are transported in both directions across the vesicle membrane. It is suggested that coupling of substrate transport to metabolic energy via transient ionic gradients may be a general phenomenon in procaryotes.
    Keywords: Life Sciences (General)
    Type: Biochemistry; 16; 14; 3227-3235
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  • 18
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    Unknown
    In:  CASI
    Publication Date: 2019-08-17
    Description: Linear ionene polyquaternary cationic polymeric segments are bonded by means of the Menshutkin reaction (quaternization) to biocompatible, extremely small, porous particles containing halide or tertiary amine sites which are centers for attachment of the segments. The modified beads in the form of emulsions or suspensions offer a large, positively-charged surface area capable of irreversibly binding polyanions such as heparin, DNA, RNA or bile acids to remove them from solution or of reversibly binding monoanions such as penicillin, pesticides, sex attractants and the like for slow release from the suspension.
    Keywords: Life Sciences (General)
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  • 19
    Publication Date: 2019-07-13
    Description: This bibliography is the sixth annual supplement to the comprehensive bibliography on the same subject which was published in Space Life Sci.We would like to draw attention to a recently published cumulative bibliography on this same subject: Biochemical Origin of Life: Chemistry and Life. Soil and Water and Its Relationship to Origin of Life. MR - Studies of Prebiotic Polypeptides. Energy, Matter, and Life. Prospects for the Future Orientation of Scientific Research. Photochemical Formation of Self Sustaining Coacervates. Photochemical Formation of Self-Sustaining Coacervates. Comparative Study of Abiogenesis of Cysteine and Other Amino Acids Catalyzed by Various Metal Ions. Protein Structure and the Molecular Evolution of Biological Energy Conversion. Origin of Life. Clues from Relations Between Chemical Compositions of Living Organisms and Natural Environments. Shock Synthesis of Amino Acids II.', Origins of Life 6(1-2). Dynamics of the Chemical Evolution of Earth's Primitive Atmosphere. The Mechanisms of Amino Acids Synthesis by High Temperature Shock-Waves. Theory of Chemical Evolution. Physical Foundations of Probability of Biogenesis.
    Keywords: Life Sciences (General)
    Type: Origins of Life; 8; 59-66
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  • 20
    Publication Date: 2019-08-17
    Description: Ionene polymers of the structure ##STR1## WHERE X AND Y ARE INTEGERS FROM 3 TO 16, Z.sup.- is an anion such as a halogen and n is an integer from 50 to 150 are found to bind negatively charged mammalian cells such as malignant cells and can be utilized to selectively inhibit the growth of malignant cells in vitro.
    Keywords: Life Sciences (General)
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  • 21
    Publication Date: 2004-12-03
    Description: Effects of thyroxine on temperature and metabolism during exercise were studied in dogs after beta-adrenergic blockade. Dogs performed 60 min treadmill exercise of moderate intensity 5 and 72 h following thyroxine injected s. c. in a single dose of 0.1 mg/kg b.w. Thyroxine increased significantly the lipolytic response to exercise as well as blood lactate (LA) concentrations and rectal temperature (T(sub re)) during exercise as early as 5 h following the hormone administration. The changes became more pronounced 72 h after the injection. At rest T(sub re), blood FFA (free fatty acid) and LA levels in the thyroxine-treated dogs did not differ from the control values, and blood glucose was slightly, but significantly higher. Propranolol given intravenously in a dose of 0.25 mg/kg at 30 min of the exercise performed 72 h following thyroxine injection abolished the plasma FFA rise, and inhibited to a certain extent increases in T(sub re) and blood LA concentrations during the next 30 min of exercise.
    Keywords: Life Sciences (General)
    Type: Acta Physiol. Pol.; Volume 27; No. 1; 33-38
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  • 22
    Publication Date: 2019-07-17
    Description: Specific mechanisms underlying weightless and hypodynamic bone loss are obscure. A principal relationship which must be affected is the balance between bone formation and bone resorption rates. In order to better define the influence of those parameters on bone loss, and also to develop measurements in other species as a useful adjunct to human research, studies were undertaken with experimental monkeys. Tests were conducted with a total of 6 adult male monkeys, weighing 10-13 kg, and approximately 10-12 yrs. of age to evaluate specifically bone formation rate during the development of disuse osteoporosis and osteopenia. Three animals were restrained in a semi-recumbent position for six months; three animals served as normal caged controls. Food intake (Purina) was held relatively constant at 200g/day for each animal. Using a Norland Bone Mineral Analyzer, bone mineral losses of 3.5 to 6% were seen in the mid-shaft of the tibia and in the distal radius. Bone loss was confirmed radiographically, with observation of thinning of the proximal tibial cortex and trabeculae in the calcaneus. Bone formation rate was determined using standard Ca-47 kinetics under metabolic balance conditions. After six months of restraint, accretion was 7.2-13.2 mg Ca/kg/day, compared to 3.2-4.1 mg Ca/kg/day in caged controls and 3-8 mg Ca/kg/day in normal adult humans. Fecal and urine calcium was 25-40% higher in restrained animals than in controls. Dietary calcium absorption decreases during restraint, and calcium turnover increases, implying a rise in bone resorption rate concommitant with the observed rise in bone accretion rate. Further studies dealing specifically with bone resorption are underway to define this more fully.
    Keywords: Life Sciences (General)
    Type: Physiologist; 19; 3
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  • 23
    Publication Date: 2019-07-12
    Description: A process for determining the susceptibility of microorganisms to antibiotics involves introducing a diluted specimen into discrete quantities of a selective culture medium which favors a specific microorganism in that the microorganism is sustained by the medium and when so sustained will change the optical characteristics of the medium. Only the specific microorganism will alter the optical characteristics. Some of the discrete quantities are blended with known antibiotics, while at least one is not. If the specimen contains the microorganisms favored by the selective medium, the optical characteristics of the discrete quantity of pure selective medium, that is the one without antibiotics, will change. If the antibiotics in any of the other discrete quantities are ineffective against the favored microorganisms, the optical characteristics of those quantities will likewise change. No change in the optical characteristics of a discrete quantity indicates that the favored microorganism is susceptible to the antibiotic in the quantity.
    Keywords: Life Sciences (General)
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  • 24
    Publication Date: 2019-07-17
    Description: During the purification of the "aerobic" acetyl-CoA synthetase (ACS) of Saccharomyces cerevisiae, strain LK2Gl2, it was noted that stronge at 4 C resulted in the loss of enzyme activity within 24 hr. Similar losses were observed during column chromatography. Addition of boiled extracts from either aerobic or anerobic cells completely prevents this. The stabilizing factor (SF) in these extracts is non-dialyzable and organic in nature. SF is excluded on G-25 and G-50 Sephadex columns and is slightly retarded on G-75 columns. On G-100 columns, SF elutes as a peak exactly coincident with that of cytochrome c, indicating a molecular weight of 13,000. SF activity was not destroyed by Pronase treatment, was adsorbed onto Norite, and absorbed in the UV with a single maximum at 260 nm. The action of SF could be replaced by a number of nucleotides. At 0.01 M, the order of effectiveness was: ATP〉ADP〉AMP〉GTP〉CTP〉/=UTP〉XTP. Even at 2 x 10(exp -4) M, ATP and ADP, but not AMP, cyclic AMP, adenosine or adenine, were effective in stabilizing this ACS. The mechanism of stabilization by ATP and AMP appears to be the same, since AMP competitively inhibited the ACS with respect to ATP in in vitro assays, while ADP gave a mixed type of inhibition, thus indicating a different mechanism. ACS from nonaerobic cells is also unstable in the absence of SF but, unlike aerobic ACS, is not affected by ATP or other nucleotides.
    Keywords: Life Sciences (General)
    Type: Federation Proceedings; 35; 7
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  • 25
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    Universität Göttingen,Abteilung Bodenphysik
    In:  Universität Göttingen
    Publication Date: 2024-04-01
    Description: research
    Keywords: Humus ; Bodenanalyse ; Basalt ; Ausgangsgestein ; Braunerde ; Türkei ; Deutschland
    Language: German
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  • 26
    Publication Date: 2011-08-23
    Description: Blockade of beta receptors inhibited thyroxine-induced increases in T(sub re) blood FFA and LA levels during exercise in dogs.
    Keywords: Life Sciences (General)
    Type: ; 68-69
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  • 27
    Publication Date: 2019-07-13
    Description: We have discovered a quantitative aging pattern in mouse urine vapor. The diagnostic power of the pattern has been found to be high. We hope that this pattern will eventually allow quantitative estimates of physiological age and some insight into the biochemistry of aging.
    Keywords: Life Sciences (General)
    Type: Exp. Geronl.; 11; 11-16
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  • 28
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    Unknown
    In:  Other Sources
    Publication Date: 2019-08-14
    Description: Biosatellite B was launched from Cape Kennedy, Florida, on a two-stage DELTA launch vehicle at 6:04 p.m. on 7 September, 1967. Approximately nine minutes later the 435 kg spacecraft biological laboratory was placed into a satisfactory 315 km near-circular earth orbit, successfully separated from the launch vehicle's second stage and was designated Biosatellite II. The scientific payload consisting of thirteen selected general biology and radiation experiments were subjected to planned, carefully controlled environmental conditions during 45 hours of earth-orbital flight. The decision was made to abbreviate the scheduled 3-day mission by approximately one day because of a threatening tropical storm in the recovery area, and a problem of communication with the spacecraft from the tracking stations. Highest priority was placed on recovery which was essential to obtain the scientific results on all the experiments. The operational phase of the mission came to a successful conclusion with the deorbit of the recovery capsule, deployment of the parachute system and air recovery by the United States Air Force. The 127 kg recovery capsule was returned to biology laboratories at Hickam Air Force Base, Hawaii, for disassembly and immediate inspection and analysis of the biological materials by the experimenters. It was evident immediately that the quality of the biology was excellent and this fact gave promise of a high return of scientific data. The environmental conditions provided to the experimental material in the spacecraft, provisions for experimental controls, and operational considerations are presented as they relate to interpretation of the experimental results.
    Keywords: Life Sciences (General)
    Type: Life sciences and space research (ISSN 0075-9422); 7; 49-61
    Format: text
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  • 29
    Publication Date: 2015-08-22
    Description: Spore forming and nonspore forming bacteria survival under diurnal freeze-thaw conditions, determining possibility of earth organism contamination of Mars environment
    Keywords: Life Sciences (General)
    Type: Space Life Sciences; Volume 1; p. 113-117
    Format: text
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  • 30
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    Unknown
    In:  CASI
    Publication Date: 2019-06-27
    Description: No abstract available
    Keywords: Life Sciences (General)
    Type: NASA-TM-X-61984
    Format: application/pdf
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  • 31
    Publication Date: 2019-08-14
    Description: Four female and two male chimpanzees were secured in various positions on a Daisy Decelerator and subjected to forces ranging from 54 to 180 G. It was found that forces in excess of 54 G may rupture the tympanic membranes and cause subepithelial hemorrhages in the middle ear. The majority of cases showed proteinaceous material, with or without cells in the petrous air spaces. When exposed to forces above 119 G, there was engorgement and often rupture of the pericarotid venous plexus. When supine, distortion of both superior and posterior semicircular canals was found. With forces in excess of 54 G, the cupulae of the cristae ampullaris were either elevated or destroyed. The hair processes were also often broken off. The otolithic membranes, especially of the maculae utriculi were also elevated or otherwise distorted and the saccule was often partially collapsed. In several instances, there was an overabundance of a proteinaceous substance in the lumina of the vestibular apparatus and in the cochlear ducts with their associated scalae. In half the cases, the cochlear duct was narrowed by the depression of the vestibular membrane. Although there seems to be considerable individual variation in ability to withstand these forces, neither age, sex nor weight appear to directly influence the results. The possible sources for the materials found both in the air cells and labyrinth are discussed.
    Keywords: Life Sciences (General)
    Type: NASA-CR-80719 , ARL-TR-66-15
    Format: application/pdf
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  • 32
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    Unknown
    In:  FAO Plant protection Bulletin, 13(5), p.97-108
    Publication Date: 1965
    Description: Einordnung einiger Epidemien von Schwarzrost, Braunrost, Gelbrost und Charakterisierung der allgemeinen Witterungsansprüche der Krankheiten KATASTER-BESCHREIBUNG: KATASTER-DETAIL:
    Keywords: Deutschland ; ca. 1920-1955 (keine Vollständigkeit, nur einzelne Jahre!) ; Getreide ; Pflanzenkrankheit ; Weizen
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