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  • wheat  (55)
  • DSC  (48)
  • Fisheries
  • General Chemistry
  • Theoretical, Physical and Computational Chemistry
  • Springer  (105)
  • 1995-1999  (105)
  • 1945-1949
  • 1940-1944
  • 1996  (105)
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  • 1995-1999  (105)
  • 1945-1949
  • 1940-1944
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  • 1
    ISSN: 1573-0832
    Keywords: Fusarium ; mycotoxins ; occurrence ; trichothecenes ; wheat ; zearalenone ; deoxynivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Wheat for human consumption (140 samples) was collected after harvest from all regions of Bulgaria. The 1995 crop year was characterized by heavy rainfall in the spring and summer months. The internal mycoflora of wheat samples was dominated by Fusarium spp. and Alternaria spp., and storage fungi were rarely present. The samples were analysed for contamination with Fusarium mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), T-2 Toxin (T-2), diacetoxyscirpenol (DAS), and zearalenone (ZEA), using enzyme immunoassay methods. DON and ZEA were the predominant toxins, with a contamination frequency of 67% and 69%, respectively. The average levels of these toxins in positive samples were 180 μg/kg (DON) and 17 μg/kg (ZEA), maximum concentrations were 1800 μg kg−1 and 120 μg kg−1, respectively. Acetyl derivatives of DON, namely 3-AcDON and 15-AcDON, were found in 2.1 % and 0.7% of the samples, at at maximum level of about 100 μg kg−1. Only one sample was positive for T-2 (55 μg/kg), DAS was not detected. This is the first report about the natural occurrence of a range of Fusarium mycotoxins in wheat for human consumption in Bulgaria.
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  • 2
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    Nutrient cycling in agroecosystems 46 (1996), S. 225-234 
    ISSN: 1573-0867
    Keywords: long-term experiment ; maize ; wheat ; fertilizers ; farm yard manure ; weedicide application ; yield sustainability ; zinc deficiency ; nutrient uptake ; cropping sequence ; organic carbon build-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term field experiments play an important role in understanding the complex interactions of plants, soils, climate and management and their effects on sustainable crop production. A long-term fertilizer experiment with maize-wheat-cowpea (fodder) is in progress since 1971 at Punjab Agricultural University farm Ludhiana, India. The experimental result for the first 21 years showed that application of N alone or in combination with P did not produce as much maize and wheat grains as the application of N, P and K together. Eight years after the start of the experiment, the optimal levels of N, P and K application (100% NPK) were unable to sustain the similar (maize) yield level as before because of Zn deficiency. Whereas in FYM amended plots the Zn deficiency did not appear and the higher crop yields could be sustained. The chemical control of weeds could not sustain the maize productivity at the same level as the manual removal of weeds. It was concluded that the high level of crop production can be sustained with the application of N, P and K under intensive cropping system provided deficiency of any of the micronutrient does not crop up. The deficiency of Zn is most likely to occur in semi-arid light textured alluvial soils under intensive cropping without the addition of farm yard manure/organic manures. In maize based cropping systems, manual control of weeds may be preferred to the chemical one. Addition of FYM in conjunction with 100% NPK is most beneficial both from bio-physical and economic point of view.
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  • 3
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    European biophysics journal 24 (1996), S. 293-299 
    ISSN: 1432-1017
    Keywords: Phase transition ; NMR ; DSC ; X-ray diffraction ; Bonnet Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Ternary systems of palmitoyl-oleoyl-phosphatidylcholine (POPC) and the non-ionic surfactant C12EO2 (di-ethylene-oxide-mono-dodecyl-ether) in water have been studied with optical microscopy, NMR, DSC and X-rays from ambient temperatures to 45 °C. Below 29 °C the system is in the lamellar liquid crystalline state. Between 30 and 32 °C it transforms into a cubic Ia3d structure which converts into the cubic Pn3m phase at 39 °C. The transitions are fully reversible. An epitaxial relationship between all three phases was found, which is an elegant and convenient way to rearrange molecules from lamellar bilayers to a network of curved surfaces. The la3d (Q230) to Pn3m (Q224) transition occurs without measurable enthalpy change. This, together with the metric relation of 1.60 between the cubic lattice constants is strong evidence for a Bonnet transformation, where the structural changes occur without change in curvature. The potential significance of the cubic phases as intermediate structures for biological processes, e. g. transport across a bilayer or fusion of membranes, are discussed.
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  • 4
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    Environmental management 20 (1996), S. 523-539 
    ISSN: 1432-1009
    Keywords: Water control ; Floodplain ; Fisheries ; Bangladesh ; Chandpur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Bangladesh is a very flat delta built up by the Ganges—Brahmaputra—Meghna/Barak river systems. Because of its geographical location, floods cause huge destruction of lives and properties almost every year. Water control programs have been undertaken to enhance development through mitigating the threat of disasters. This structural approach to flood hazard has severely affected floodplain fisheries that supply the major share of protein to rural Bangladesh, as exemplified by the Chandpur Irrigation Project. Although the regulated environment of the Chandpur project has become favorable for closed-water cultured fish farming, the natural open-water fishery loss has been substantial. Results from research show that fish yields were better under preproject conditions. Under project conditions per capita fish consumption has dropped significantly, and the price of fish has risen beyond the means of the poor people, so that fish protein in the diet of poor people is gradually declining. Bangladesh is planning to expand water control facilities to the remaining flood-prone areas in the next 15–20 years. This will cause further loss of floodplain fisheries. If prices for closed-water fish remain beyond the buying power of the poor, alternative sources of cheap protein will be required.
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  • 5
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    Journal of thermal analysis and calorimetry 47 (1996), S. 35-49 
    ISSN: 1572-8943
    Keywords: bone cement ; DSC ; kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Bone cements are widely used for the fixation of metallic prostheses in orthopaedics and to form replacements for skull defects in neurosurgery. Acrylic bone cements are based on a mixture of methyl methacrylate (MMA) and a fine powder of polymethyl methacrylate (PMMA). The polymerization of the bone cement occurs in contact with the bone and the prosthesis which act as the boundaries of a bulk polymerization reactor. The kinetic behaviour of the bone cement plays a fundamental role for the final performance of the implant. In this paper, the isothermal and non-isothermal polymerization behaviour of a commercial bone cement is described. A simple phenomenological model, accounting for the autoacceleration ffect, for a diffusion controlled termination mechanism and for the reaction between inhibitor and initiator, is proposed. The reaction kinetics is analysed by DSC. DSC data are used for the determination of the rates of polymerization under isothermal and non-isothermal conditions. The experimental data are processed to calculate the parameters of the proposed phenomenological kinetic model. The analytical and numerical details related to the integration of the model are discussed.
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  • 6
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    Journal of thermal analysis and calorimetry 47 (1996), S. 181-194 
    ISSN: 1572-8943
    Keywords: DSC ; gelatinization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Data obtained by dynamic mechanical analysis and DSC analysis of durum wheat dough are presented and discussed. Doughs with water contents ranging from 45 to 55% (w/w) were subjected to sinusoidal shearing by means of a dynamic mechanical spectrometer (Rheometrics, RFS2) equipped with parallel plate geometry, 0.1 strain amplitude and 1 rad/min frequency. The tests were carried on in temperature sweep mode at a heating rate of 2°C min−1. Wheat samples with water contents in the range between 7.5 and 37.5% and doughs with 37.5% moisture content were mixed for different times and subjected to DSC analysis (Perkin-Elmer, DSC-7) at a heating rate of 20°C min−1. Dynamic mechanical analysis revealed that the relationship between the dynamic properties of the dough and the temperature was modified as the water content of the dough increased and was quite different from that for gluten. A similar response was observed in the course of temperature scans made by means of DSC. These experimental findings suggest that the water-starch interaction in the presence of a protein matrix is affected by the availability of water and that the protein system is a competitor with respect to starch.
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  • 7
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    Journal of thermal analysis and calorimetry 47 (1996), S. 543-557 
    ISSN: 1572-8943
    Keywords: compensation effect ; DSC ; kinetics ; TG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Four computer programs as well as one demo-version for non-linear evaluation of kinetic data in thermal analysis and calorimetry, were presented. The multi-task program TA-kin meets all mathematical requirements for solving the numerical assignments. It is shown that the so-called compensation effect is due to the mathematical structure of the Arrhenius equation. Several applications of TA-kin to a lot of DSC- and TG-measurements and isoperibolic batch experiments as well as adiabatic semi batch experiments realized by precision calorimetry have been discussed.
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  • 8
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    Journal of thermal analysis and calorimetry 47 (1996), S. 685-695 
    ISSN: 1572-8943
    Keywords: DSC ; polymers ; resins ; TG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The paper describes the co-curing studies of ethynyl and ethenyl end-capped imide resins. The effect of composition and chemical structure of ethenyl end-capped resins (nadicimides) on thermal behavior of ethynyl end-capped resins was evaluated using DSC and thermogravimetric analysis. An increase in char yield was observed on co-curing of few resin formulations. A mechanism has been proposed to account for this observation.
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  • 9
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1213-1228 
    ISSN: 1572-8943
    Keywords: cassava starch ; DSC ; starch thermal properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Order-disorder transitions were investigated in native cassava starch at intermediate moisture contents (35 to 60% wt. water), using Differential Scanning Calorimetry (DSC) and dynamic Wide Angle X-ray Diffractometry (WAXS) with a synchrotron radiation source. The gelatinization of granules occurs as a cooperative process, due to constraints induced in crystallites by the amorphous areas. Variations of water content (water volume fraction from 0.28 to 0.86) and heating rate (0.2–10
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  • 10
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1179-1200 
    ISSN: 1572-8943
    Keywords: DSC ; isothermal microcalorimetry ; retrogradation ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In the present study, isothermal microcalorimetry was introduced as a tool to investigate properties of starch retrogradation during the first 24 h. The study was made on purified amylose and amylopectin from corn, as well as on native starches, such as wheat, potato, maize, waxy maize and amylomaize, differing in their amylose content. The results were obtained in the form ofP-t traces (thermal powervs. time), and integration of these traces gave a net exothermic enthalpy of reaction, caused by the crystallization of amylose and amylopectin. TheP-t traces reflected the quantities of amylose and amylopectin in the starch studied. Depending on the amylose content and the botanical source of the starch, the rate of crystallization of amylose was high and predominated over that of amylopectin during the first 5–10 h. The contribution from amylose crystallization to the measured exothermic enthalpy was very substantial during this period. After ∼10 h, amylose crystallized at a lower constant rate. During the first 24 h, amylopectin crystallized at a low steady rate. The exothermic enthalpies obtained by the isothermal microcalorimetric investigations during the first 24 h of retrogradation were generally low in relation to the endothermic melting enthalpies observed by differential scanning calorimetry (DSC) measurements after 24 h of storage. The discrepancies in enthalpy values between the two methods are discussed in relation to phase separation and the endothermic effects owing to the decrease in polymer-water interactions when polymer-rich regions in the starch gel separate. Besides the exothermic enthalpies obtained, theP-t traces also made it possible to study the initial gelation properties of amylose from different botanical sources. The present study further demonstrated that isothermal microcalorimetry can provide a possible way to investigate the antistaling effect of certain polar lipids, such as sodium dodecylsulphate (SDS) and 1-monolauroyl-rac-glycerol (GML), when added to starches of different botanical origin. The net exothermic heat of reaction for starch retrogradation during the first 24 h was decreased when GML or SDS was added to the starch gels. The recordedP-t traces also showed how the effect of the added lipid influenced different periods during the first 24 h of starch retrogradation, and that the effect depended mainly on the amylose content, the botanical source of the starch, and the type of lipid used. When GML or SDS was added to waxy maize, the isothermal microcalorimetric studies clearly indicated some interaction between amylopectin and the polar lipids. These results concerning the action of anti-staling agents are further discussed in relation to the helical inclusion complexes formed between amylose-polar lipid and amylopectin-polar lipid.
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  • 11
    ISSN: 1572-8943
    Keywords: cooling/heating rate ; DSC ; fragility parameter ; glass transition temperature ; sorbitol-fructose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The glass transition temperatures of sorbitol and fructose were characterized by four points determined on DSC heating thermograms (onset, mid-point, peak and end-point), plus the limit fictive temperature. The variations of these temperature values, observed as functions of cooling and heating rates, were used to determine the fragility parameter, as defined by Angell [1] to characterize the temperature dependence of the dynamic behavior of glass-forming liquids in the temperature range above the glass transition. The apparent activation energy values, determined for the different temperatures studied, were similar for fructose and sorbitol. These values were compared to data obtained from other techniques, such as mechanical spectroscopy. The variations of the apparent activation values, observed in experiments involving cooling and heating at the same rate, slow cooling followed by rate-heating, or rate-cooling followed by fast heating, were explained by aging effects occurring during the heating step.
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  • 12
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1545-1565 
    ISSN: 1572-8943
    Keywords: cocoa butter ; fat crystallization ; DSC ; fat polymorphism ; fat structure ; fats ; lard ; milk fat ; triacylglycerols ; X-ray diffraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The thermal behavior of three ural fats (displaying very different composition), cocoa butter (CB)2, lard, and a stearin obtained from anhydrous milk-fat (AMF) fractionation, were studied by both DSC and X-ray diffraction as a function of temperature (XRDT). To perform temperature explorations between −30
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  • 13
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    Journal of thermal analysis and calorimetry 46 (1996), S. 5-14 
    ISSN: 1572-8943
    Keywords: cadmium phenoxyacetate ; complexes ; DSC ; mercury(II) phenoxyacetate ; TG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Solid cadmium and mercury(II) phenoxyacetates were prepared and investigated by DSC and TG techniques. The cadmium salt decomposed in two steps with the loss of 1.5 water molecules at first and the successive formation of CdCO3 as final product. Δdeh H * associated with the loss of one water molecule was compared with the corresponding values obtained for other phenoxyacetates previously studied and the obtained results were discussed. Anhydrous mercury(II) phenoxyacetate gave, on heating, HgCO3 which successively decomposed with the formation of gaseous products and a little amount of solid residue.
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  • 14
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    Journal of thermal analysis and calorimetry 46 (1996), S. 177-186 
    ISSN: 1572-8943
    Keywords: activation energy ; chalcogenide glass ; DSC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Results of differential scanning calorimetry (DSC) at different heating rates on Se2Ge0.2Sb0.8 chalcogenide glass are reported and discussed. As the heating rate (α) changed, also the glass transition temperature (T g) and onset temperature of crystallization (T c) changed. As the value of the transition activation energyE t changed, the crystallization fraction (χ), heat flow (Δq and the crystallization peak temperature (T p) also changed. The value of the effective activation energy of crystallizationE c was calculated by means of six different methods. The Se2Ge0.2Sb0.8 chalcogenide glass has two crystallization mechanisms, a one-dimensional and an other surface crystallization growth. The average value ofE t for Se2Ge0.2Sb0.8 is equal to 194.95±3.9 kJ·mol−1 and the average value ofE c is equal to 164±3.3 kJ·mol−1.
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  • 15
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    Journal of thermal analysis and calorimetry 46 (1996), S. 237-243 
    ISSN: 1572-8943
    Keywords: DSC ; DTG ; polyacetylene films ; TMA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Polyacetylene films irradiated byγ-rays up to 100 MRad were studied by means of TMA, DTG and DSC methods. It is shown that as the irradiation dose increases the concentration of topological branching knots into the polymer chains and theT g values decrease, the total mass loss and the enthalpy of the thermal isomerization reaction also decrease.
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  • 16
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    Journal of thermal analysis and calorimetry 46 (1996), S. 619-626 
    ISSN: 1572-8943
    Keywords: crystallization ; DSC ; filled polypropylene ; nucleation ; structural modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The influence of high concentration of BaSO4 as nucleating agent on crystallization of fiber-forming polypropylene was studied by DSC. The work presents experimental and calculated values of melting and crystallization enthalpies of filled polypropylene and the influence on the formation of interface interactions between filler and polymers. These results show minimal interactions of components (BaSO4 and polypropylene) under experimental conditions.
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  • 17
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    Journal of thermal analysis and calorimetry 46 (1996), S. 643-679 
    ISSN: 1572-8943
    Keywords: DDTA ; DSC ; macromolecules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract This review traces the development of thermal analysis over the last 40 years as it was experienced and contributed to by the author. The article touches upon the beginning of calorimetry and thermal analysis of polymers, the development of differential scanning calorimetry (DSC), single run DSC and other special instrumentations, up to the recent addition of modulation to calorimetry. Many new words and phrases have been introduced to the field by the author and his students, leaving a trail of the varied interests one can have over 40 years. It began with “cold crystallization” and most recently the term “oriented, intermediate phase” was coined, creating in-between: “extended chain crystals,” the “irreversible thermodynamics of melting of polymer crystals,” “dynamic differential thermal analysis” (DDTA), “the rule of constant increase ofC p per mobile bead within a molecule at the glass transition temperature,” “superheating of polymer crystals,” “melting kinetics,” “crystallization during polymerization,” the “chain-folding principle, “molecular nucleation,” “rigid amorphous phase,” a “system of classifying molecules,” “macroconformations,” “amorphous defects,” “rules for the entropy of fusion based on molecular shape and flexibility,” “single-molecule single-crystals,” “a system of classifying phases and mesophases,” and “condis phase.”
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  • 18
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    Journal of thermal analysis and calorimetry 46 (1996), S. 795-808 
    ISSN: 1572-8943
    Keywords: DSC ; kinetics ; liquid crystalline polymer ; optical transmittance ; polycarbosilane ; side-chain mesogen ; transition parameters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract This paper is concerned with an analysis of the thermodynamics and kinetics of mesophase formation by cooling from the isotropic state of side-chain liquid crystalline polycarbosilanes containing spacers in the range from 3 to 11 CH2-groups. The polymers are characterized by their thermotropic behaviour as far as temperature, enthalpy and entropy of the transitions are concerned. The kinetics was followed by optical and calorimetric methods. Longer spacer length leads to more perfect ordering in the mesophase, higher isotropization temperatures, and lower glass transition temperatures. The Avrami and Ozawa formalism to describe the transition kinetics to the mesophase from the isotropic state cannot be interpreted as the nucleation and growth mechanism known from crystallization.
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  • 19
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1201-1212 
    ISSN: 1572-8943
    Keywords: aging ; amylopectin ; DSC ; gels ; glass transition ; recrystallization ; retrogradation ; rice starch ; sugars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Thermal characterization of gelatinized binary rice starch-water and ternary starch-sugar-water gels before and after aging was carried out using differential scanning calorimetry. The glass transition temperature of the maximally freeze-concentrated solution (T′g) in both fresh and aged gels was observed to decrease progressively with increasing sugar concentration. Aging of the gels generally shiftedT′g to higher temperatures, but had little or no effect on the ice melting peak temperature (T m). The presence of various sugars could either accelerate or retard starch (amylopectin) recrystallization, depending on the type and concentration of sugar, as well as on starch/water ratio. A hypothesis based on the dual antiplasticizing-plasticizing effects of sugars was postulated to explain the observed effects. Of the sugars studied, xylose and fructose appeared to display exceptional retardative and accelerative effects, respectively, on retrogradation.
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  • 20
    ISSN: 1572-8943
    Keywords: DSC ; gelatinisation ; potato starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Gelatinisation temperatures as a function of moisture content were determined for potato starch. The native starch was then hydrothermally treated at a temperature 3% (Kelvin degrees) below the gelatinisation peak temperature and at moisture levels varying from 20 to 67% (by weight). Gelatinisation temperatures, temperature ranges and enthalpy values were affected for all treated samples. However, two sample populations could be distinguished: those samples treated under ‘limited’ moisture conditions and other samples treated in the presence of ‘extragranular’ moisture. A two-step hydrothermal treatment further increased the gelatinisation temperature, but the effect of the second step was small in comparison to that of the first.
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  • 21
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1299-1314 
    ISSN: 1572-8943
    Keywords: baked goods ; cookies ; crackers ; DSC ; pretzels ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract We describe an application of DSC as an analytical ‘fingerprinting’ method that has been used to characterize the thermal properties of wheat starch in low-moisture, wheat-flour-based baked products, including cookies, crackers, and pretzels. This use of DSC has enabled us to relate starch thermal properties, on the one hand, to starch structure, and on the other hand, to starch functionality, in terms of baking performance and finished-product quality.
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  • 22
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1339-1360 
    ISSN: 1572-8943
    Keywords: bread crumb ; bread dough ; DSC ; hydrocolloid ; mechanical properties ; pentosans ; protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The effects of hydrocolloids (guar and locust bean gums), soluble pentosans, and whey proteins on staling of bread crumb were investigated by means of DSC, rheometry, and image analyis. One current hypothesis, that these ingredients would behave as “water binders” and, at least the former two, as anti-staling agents, was indeed confirmed, although this action might be indirect. All the samples considered showed an exothermic DSC peak preceding the endotherm of the amylopectin fusion. According to a previous work, this signal was attributed to a water-dependent cross-linking process that would involve next-neighbouring polymer chains. To check the effect produced by molecular modifications that were expected to increase the water uptake of these ingredients, doughs containing added succinylated pentosans and whey proteins, and a polycarboxylate polymer, PEMULEN TR-1, were examined. These modifications enhanced starch retrogradation and yielded a firmer crumb. It was tentatively concluded that some direct interaction between these modified molecules and the crumb polymers might have taken place. In line with the food polymer science approach, the use of Time-Temperature-Transformation (TTT) diagrams is also discussed.
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  • 23
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1513-1525 
    ISSN: 1572-8943
    Keywords: Β-lactoglobulin ; DSC ; protein modification ; thermal properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The thermal properties of Β-lactoglobulin (Β-LG) were studied by differential scanning calorimetry (DSC) under different medium conditions.pH, neutral salts, protein perturbants, and polyols all affected the DSC characteristics of Β-LG. Acylation with fatty acids also changed the thermal properties, particularly peak width at half-height. The results suggest that the structural stability of Β-LG is controlled by non-covalent forces, particularly electrostatic and hydrophobic interactions. Disulfide bonds did not contribute to the thermal response of Β-LG. Fatty N-acyl-amino acids caused marked increases in thermal stability and decreases in denaturation enthalpy, and additional peaks were observed in the presence of some palmitoyl derivatives.
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  • 24
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    Journal of thermal analysis and calorimetry 46 (1996), S. 347-352 
    ISSN: 1572-8943
    Keywords: DSC ; optical densities ; thermal stability ; three-component interpolymer complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new type of three-component interpolymer complexes (3IPCM) formed by two similarly charged polyelectrolytes and an oppositely charged low molar mass compound was studied by DSC, NMR and X-ray methods. The low molar mass monobasic compounds in these complexes act as mediators. This type of complexes differs from earlier-obtained 3IPCM, which contained a dibasic low molar mass mediator. The present 3IPCM were obtained from two polymers (polyacrylic acid and sodium polyphosphate) and bases such as 4-vinylpyridine and 2-methyl-5-vinylpyridine.
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  • 25
    ISSN: 1572-8943
    Keywords: cure kinetics ; DSC ; epoxy resins ; gelation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract By employing differential scanning calorimetry (DSC) we have studied the kinetic of the cure reaction for a system containing a diglycidyl ether of bisphenol A (DGEBA) and 1,3-bisaminomethylcyclohexane (1,3-BAC) as a curing agent, using an isothermal approach over the temperature range of 60–110°C. We have determined the reached conversions at several cure temperatures and the reaction rates. The results showed that this cure reaction is autocatalytic. The experimental data were compared with the autocatalytic model proposed by Kamal, which includes two rate constants and two reaction orders. This model gives a good description of cure kinetics up to vitrification point. The activation energies for these rate constants were 44-57 kJ mol−1. From the gel time measurements the value obtained for the overall activation energy was 49.5 kJ mol−1.
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  • 26
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    Journal of thermal analysis and calorimetry 46 (1996), S. 465-470 
    ISSN: 1572-8943
    Keywords: ABS polymer ; DSC ; lifetime estimation ; thermooxidation
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The service life of ABS polymer, stabilized by 2-(3,5-di-tert-butyl-4-hydroxyanilino)-4,6-bis(octylthio)-1,3,5-triazine and containing 50% of a modifying rubber component, was estimated from oxidative induction times measured by DSC in isothermal mode in the temperature interval 140–170°C. The lifetime of ABS powder at the actual temperature of drying was predicted by linear extrapolation according to Arrhenius. However, the extrapolated value was much longer than the real lifetime determined from the long-term oven aging tests at 70 and 90°C, simulating the industrial drying process. The effect of changes in the apparent activation energy of oxidation due to antioxidant consumption during polymer aging is discussed.
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  • 27
    ISSN: 1572-8943
    Keywords: complexes ; DSC ; guest-host interactions ; X-ray
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The stoichiometry of thermal decomposition and the thermochemistry were studied for [NiL4(NCS)2] (I) as a host complex, and for its clathrates of type [NiL4(NCS)2]·2G, where L=4-ethylpyridine and guest molecule G=1-methylnaphthalene in clathrate (II), 1-chloronaphthalene in (III) or 1-bromonaphthalene in (IV). For I, the loss of volatile components proceeds in three steps (−2L, −L, −L); the first steps for II–IV also involve the release of G (−2G, −2L). DSC and X-ray powder measurements indicated a phase transition in the host lattice, and allowed differentiation of the escape of G and L molecules. The enthalpy changes give the following sequence of thermodynamic stability for the studied chlathrates: I〉II〉III.
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  • 28
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    Journal of thermal analysis and calorimetry 46 (1996), S. 753-771 
    ISSN: 1572-8943
    Keywords: crystal-crystal transitions ; crystal forms ; DMA ; DSC ; Nylon M5T ; X-ray
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Poly(2-methylpentamethylene terephthalamide) (Nylon M5T) is a new high temperature aromatic polyamide developed by Hoechst Celanese. In this paper thermal properties of Nylon M5T chips, as well as as-spun and drawn fibers were studied by DSC, DMA, hot stage microscopy and WAXS.T g of the fully amorphous Nylon M5T is 143°C when measured by DSC;T g increases with crystallinity to 151°C. The temperature dependence of the solid and melt specific heat capacities has also been determined. The heat capacity increase at the glass transition of the amorphous polymer is 103.9 J °C−1 mol−1.T g by DMA for the as-spun fiber is 155°C, for a drawn fiber is 180°C. Three secondary transitions were observed by DMA in addition to the glass transition. These correspond to a local mode relaxation of the methylene groups at −120°C, onset of rotation of the amide-groups at −65°C and the onset of the rotation of the phenylenegroups (at 63°C). The crystallinity of Nylon M5T strongly depends on the rate of cooling from the melt. The isothermal crystallization data are melt temperature dependent: two-dimensional crystallization takes place when the samples are crystallized from higher melt temperatures, and this phase changes into a spherulitic structure during cooling to room temperature. Spherulitic crystallization occurs when lower melt temperatures are used. This polymer has three crystal forms as indicated by DSC, DMA and WAXS data. The crystal to crystal transitions are clearly visible when amorphous samples are heated in the DSC, or the DMA curves of as-spun fibers are recorded. It is experimentally shown that a considerable melting of the lower temperature crystal forms takes place during the crystal to crystal transitions. The equilibrium melting point as measured by the Hoffman-Weeks method, has been determined to be 339°C.
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  • 29
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    Journal of thermal analysis and calorimetry 46 (1996), S. 871-878 
    ISSN: 1572-8943
    Keywords: DSC ; melting point ; polyethylene ; Raman-active longitudinal acoustical mode ; semi-crystalline polymers
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    Topics: Chemistry and Pharmacology
    Notes: Abstract This is a study for criteria to judge the melting point of semi-crystalline polymers from the DSC endotherm for polymer melting. Beyond standard indium DSC melting results an evaluation has been made on a series of polyethylenes for which crystal sizes were measured and predicted from Raman LAM analysis. The results confirm the conclusion of Prof. Wunderlich that the DSC content of melting is the proper basis of reporting melting points.
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  • 30
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    Journal of thermal analysis and calorimetry 46 (1996), S. 893-903 
    ISSN: 1572-8943
    Keywords: cold crystallization ; DSC ; heat capacity ; modulated temperature DSC ; poly(ethylene terephtalate)
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The modulated temperature differential scanning calorimetric method (MT-DSC) yields three temperature dependent signals, an underlying heat capacity curve from the underlying heat flow rate (corresponding to the conventional DSC signal), and a complex heat capacity curve with a real part (storage heat capacity) and an imaginary part (loss heat capacity). These curves have been measured in the cold crystallization region for poly(ethylene terephtalate) with a modified Perkin-Elmer DSC-7. The underlying curve shows the well known large exothermic crystallization peak. The storage heat capacity shows a step change which reproduces the change in heat capacity during crystallization. This curve may be used as baseline, to separate the crystallization heat flow rate from the underlying heat flow rate curve. The loss heat capacity curve exhibits a small exothermic peak at the temperature of the step change of the storage curve. It could be caused by changes of the molecular mobility during crystallization.
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  • 31
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    Journal of thermal analysis and calorimetry 46 (1996), S. 1251-1258 
    ISSN: 1572-8943
    Keywords: boiling points ; DSC ; TGA ; vapour pressure
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    Topics: Chemistry and Pharmacology
    Notes: Abstract A TGA instrument has been adapted for rapid measurement of boiling points and vapour pressure at temperatures from ambient up to 400°C and pressures from ambient down to 20 mm Hg. Samples were contained in sealed holders having a laser-drilled aperture. Several organic liquids in the 100 to 300 gMW range showed good agreement with reference vapour pressure data. Sample mass, heating rate, and use of inert diluents were important variables affecting accuracy of vapour pressure measurements.
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  • 32
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    Journal of thermal analysis and calorimetry 47 (1996), S. 957-973 
    ISSN: 1572-8943
    Keywords: DSC ; Gibbs energy ; liquid crystalline polyethers
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    Topics: Chemistry and Pharmacology
    Notes: Abstract A series of polyethers have been synthesized from 1-(4-hydroxy-4′-biphenyl)-2-(4-hydroxyphenyl)propane and α, Ω-dibromoalkanes having different numbers of methylene units [TPPs]. Both odd- and even-numbered TPPs [TPP(n=odd)s and TPP(n=even)s) exhibit multiple transitions during cooling and heating and they show little supercooling dependence, indicating close-to-equilibrium nature of these transitions. Combining the structural characterization obtainedvia wide angle X-ray diffraction powder and fiber patterns at different temperatures and the morphological observations from microscopy techniques, not only the nematic liquid crystalline phase but also highly ordered smecticF, smectic crystalG andH phases have been identified. The phase diagrams for both TPP(n=odd)s and TPP(n=even)s have been constructed [1–3]. Thermodynamic properties (enthalpy and entropy changes) during these transitions are studied based on differential scanning calorimetry experiments. The contributions of the mesogenic groups and methylene units to each ordering process can be separated and they indicate the characteristics of these processes thereby providing estimations of the transition types.
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  • 33
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1743-1753 
    ISSN: 1572-8943
    Keywords: DSC ; HSM ; oxazepam ; PEG 4000 ; solid dispersion
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    Topics: Chemistry and Pharmacology
    Notes: Abstract A thermal study using DSC and Hot Stage Microscopy (HSM) was carried out to investigate the interaction in solid state of the binary system PEG 4000 — oxazepam, and to establish their phase diagram. The eutectic composition, which melting occurs at lower temperature as compared with the pure components, has been determined. The results obtained by DSC and HSM have indicated that PEG 4000 — oxazepam mixtures displays no obvious incompatibilities, and that the system shows a typical eutectic behaviour. However because of the closeness of the melting of PEG 4000 to the eutectic temperature, it was difficult to determine precisely the eutectic composition and temperature on the basis of DSC measurements alone. The use of heats of fusion corresponding to physical mixtures allowed an estimation of the eutectic composition at 6% w/w oxazepam. Additional information of temperature (57.6
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  • 34
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1755-1758 
    ISSN: 1572-8943
    Keywords: combustion calorimetry ; DSC ; sulphamide type compounds
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Two compounds of sulphamide type:p-amino-benzene sulphonamide (I) and 3,4-dimethylisoxazol 5-sulphanylamide (II) were studied by combustion calorimetry and by differential scanning calorimetry (DSC). The enthalpies in solid state at 298,15 K of combustion, δc H m o (I)=-2788,5±1,6 kJ mol−1, δc H m o (II)=-5036±3,8 kJ mol−1 and of formation, δf H m o (I)=-458,3±1,6 kJ mol−1, δfH m o (II)=-180,1±3,8 kJ mol−1 were determined. The thermal effects concerning the melting and phase transition of this compounds were also measured.
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  • 35
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    Journal of thermal analysis and calorimetry 47 (1996), S. 1787-1803 
    ISSN: 1572-8943
    Keywords: DSC ; food components ; food microbiology ; food quality
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Differential scanning calorimetry (DSC) is the most widely used thermal analytical technique in food research and it has a great utility in quality assurance of food. Proteins are the most studied food components by thermal analysis including studies on conformation changes of food proteins as affected by various environmental factors, thermal denaturation of tissue proteins, food enzymes and enzyme preparations for the food industry, as well as effects of various additives on their thermal properties. Freezing-induced denaturation of food proteins and the effect of cryoprotectants are also monitored by DSC. Polymer characterization based on DSC of polysaccharides, gelatinization behaviour of starches and interaction of starch with other food components can be determined, and phase transitions during baking processes can be studied by DSC. Studies on crystallization and melting behaviour of fats observed by DSC indicate changes in lipid composition or help characterizing products. Thermal oxidative decomposition of edible oils examined by DSC can be used for predicting oil stability. Using DSC in the freezing range has a great potential for measuring and modelling frozen food thermal properties, and to estimate the state of water in foods and food ingredients. Research in food microbiology utilizes DSC in better understanding thermoadaptive mechanisms or heat killing of food-borne microorganisms. Isothermic microcalorimetric techniques provide informative data regarding microbial growth and microbial metabolism.
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  • 36
    ISSN: 1572-8943
    Keywords: DSC ; pharmaceutical technology ; polymer
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    Topics: Chemistry and Pharmacology
    Notes: Abstract In the present study, the effect of the molecular weight and thermal treatments on commercial polyethylene glycols (PEG) samples used in the pharmaceutical processing technology, has been analyzed using DSC and HSM. The molecular weight of these polymers range from 1500 to 200000. Thermal investigations on the melting behavior of original PEG samples (as received from the manufacturer) showed only one single melting DSC endotherm effect before 373 K. This fact was associated to the presence of only one type of polymeric chain. Using standard conditions, PEG samples were solidified from the melt at 373 K, either by flash cooling (using liquid nitrogen and an ice bath) and by slow cooling, soaked and by slow cooling at room temperature. They were further studied by DSC. It was found that after cooling, PEG with molecular weight 1500 and 15000 showed DSC thermograms with a single endothermic peak. However, thermograms for PEG 4000 and 6000 produced a splitted melting endotherm. This fact was attributed to the presence of two types of chains, that are the folded and extended chains. Ageing time influences also the shape of the DSC endothermal effects. It was concluded that the endotherms obtained after heating these PEG indicate that the thermal history determine the structure (extended or folded chain type forms) and the degree of crystallinity, as evidenced by changes in heat of fusion values, melting points and structures after crystallization. The relationships between melting enthalpies and melting points, as deduced from DSC diagrams, with molecular weight of the polymers are also presented.
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  • 37
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    Journal of thermal analysis and calorimetry 46 (1996), S. 1081-1092 
    ISSN: 1572-8943
    Keywords: chemical ionization MS ; constrained dye ; DSC ; dye-amylose inclusion complex ; Tandem MS ; TGA ; Thermal Desorption MS
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The thermal properties of a dye molecule (guest) inside the cavity of a host amylose helix were studied by TGA, DSC, and Thermal Desorption MS. The results show that the degradation temperature of dye shifts to a higher temperature by approximately 20°C.
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  • 38
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    Journal of thermal analysis and calorimetry 46 (1996), S. 1093-1111 
    ISSN: 1572-8943
    Keywords: conformational disorder ; crystal ; DSC ; glass ; glass transition ; heat capacity ; melting transition ; tetra[methyleneoxycarbonyl (2,4,4-trimethyl) pentyl] methane
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Quantitative thermal analysis was carried out for tetra[methyleneoxycarbonyl(2,4,4-trimethyl)pentyl]methane. The ester has a glass transition temperature of 219 K and a melting temperature of 304 K. The heat of fusion is 51.3 kJ mol−1, and the increase in heat capacity at the glass transition is 250 J K−1 mol−1. The measured and calculated heat capacities of the solid and liquid states from 130 to 420 K are reported and a discussion of the glass and melting transitions is presented. The computation of the heat capacity made use of the Advanced Thermal Analysis System, ATHAS, using an approximate group-vibration spectrum and a Tarasov treatment of the skeletal vibrations. The experimental and calculated heat capacities of the solid ester were compared over the whole temperature range to detect changes in order and the presence of large-amplitude motion. An addition scheme for heat capacities of this and related esters was developed and used for the extrapolation of the heat capacity of the liquid state for this ester. The liquid heat capacity for the title ester is well represented by 691.1+1.668T [J K−1 mol−1]. A deficit in the entropy and enthalpy of fusion was observed relative to values estimated from empirical addition schemes, but no gradual disordering was noted outside the transition region. The final interpretation of this deficit of conformational entropy needs structure and mobility analysis by solid state13C NMR and X-ray diffraction. These analyses are reported in part II of this investigation.
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  • 39
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    Journal of thermal analysis and calorimetry 46 (1996), S. 1113-1132 
    ISSN: 1572-8943
    Keywords: chiral molecule ; conformational disorder and motion ; crystal ; DSC ; heat capacity ; γ-gauche effect ; glass ; glass transition ; melting transition ; molecular mechanics computations ; tetra[methyleneoxycarbonyl (2,4,4-trimethyl) pentyl] methane ; solid state13C NMR ; X-ray diffraction
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    Notes: Abstract The symmetric neopolyol ester tetra[methyleneoxycarbonyl(2,4,4-trirnethyl)pentyl]methane (MOCPM) has been studied by variable-temperature solid-state13C NMR and X-ray powder diffraction and compared to molecular mechanics calculations of the molecular structure. Between melting and glass transition temperatures the material is semicrystalline, consisting of two conformationally and motionally distinguishable phases. The more mobile phase is liquid-like and is, thus attributed to an amorphous phase (≈16%). The branches of the molecules in the crystal exhibit two conformationally distinguishable behaviors. In one, the branches are well ordered (≈56%), in the other, the branches are conformationally disordered (≈28%). Different branches of the same molecule may show different conformational order. This unique character of the rigid phase is the reason for the deficit of the entropy of fusion observed earlier by DSC. In the melt, solid state NMR can identify two bonds that are rotationally immobile, even though the molecules as a whole have liquid-like mobility. This partial rigidity of the branches accounts quantitatively for the observed increase in heat capacity at the glass transition. The reason for this unique behavior of MOCPM, a small molecule, is the existence of one chiral centers in each of the four arms of the molecule. A statistical model assuming that at least two of the chiral centers must fit into the order of the crystal can explain the crystallization behavior and would require 12.5% amorphous phase, 28.1% conformational disorder, and 59.4% crystallinity, close to the observed maximum perfection.
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  • 40
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    Journal of thermal analysis and calorimetry 46 (1996), S. 1177-1189 
    ISSN: 1572-8943
    Keywords: DSC ; mercury porosimetry ; pore size distribution ; porous glass ; thermoporosimetry ; water
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The pore size distributions (PSDs) of microporous glass, which were controlled by acid leaching subsequent to phase separation of CaO-Al2O3-B2O3-SiO2 glass, were determined via both mercury porosimetry and thermoporosimetry (thermal porosimetry). As a result, the pore radii, the cumulative pore volumes, and the surface areas determined via thermoporosimetry were in good agreement with those determined via mercury porosimetry. It was revealed that thermoporosimetry could be applied to pore structure analysis for porous materials having pore sizes at least up to 58 nm in radius.
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  • 41
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    Journal of thermal analysis and calorimetry 46 (1996), S. 1339-1347 
    ISSN: 1572-8943
    Keywords: boehmite ; DSC ; gibbsite
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The dehydroxylation of gibbsite into boehmite was investigated by means of DSC analysis under non-isothermal conditions in the temperature range 453–673 K at heating rates from 2.5 to 20.0 K min−1. Mathematical analysis of the experimental DSC curves revealed the mechanism and kinetics of the gibbsite dehydroxylation process. The kinetic curvesα=f(t) andα=f(T) are sigmoidal in shape; their inflection points and the νm point of the curvesν=f(T) andν=f(T) are interrelated and are defined by the concept of a stationary point. The activation energy for the first stage of gibbsite dehydroxylation in the temperature range 453–673 K is 132.92±8.33–142.26±8.33 kJ mol−1.
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  • 42
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    Journal of thermal analysis and calorimetry 46 (1996), S. 1541-1550 
    ISSN: 1572-8943
    Keywords: DSC ; FTIR ; grafting ; IDSC ; kinetic parameters ; polymerization ; TG ; thermodynamic parameters
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The thermal polymerization of pentabromobenzyl (mono)acrylate (PBB-MA) on the surface of the inorganic fillers Mg(OH)2 and CaCO3 was studied. FTIR spectroscopy and extraction of the polymer in bromobenzene show that polypentabromobenzyl acrylate (PBB-PA) was mostly grafted on the surface of Mg(OH)2. Thermal analysis (TG, DSC, isothermal DSC (IDSC)) demonstrated an increase in polymerization starting temperature, and differences in polymerization enthalpy and apparent activation energy when an inorganic filler is added. These differences depend on the chemical composition of the filler used.
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  • 43
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    Journal of thermal analysis and calorimetry 47 (1996), S. 117-121 
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    Keywords: baseline ; DSC ; heat of fusion ; polyethylene
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    Topics: Chemistry and Pharmacology
    Notes: Abstract A technique is proposed for improving the accuracy of the heat of fusion of semicrystalline polymers by DSC. The results of three commercially available instruments are compared.
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  • 44
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    Journal of thermal analysis and calorimetry 47 (1996), S. 195-213 
    ISSN: 1572-8943
    Keywords: DMA ; DSC ; rheological impacts ; thermomechanical properties
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Thermomechanical properties of bread components can be used to characterize various events that have direct rheological impacts. The objective is to observe changes that occur during staling and toughening of a bread or similar products. In this article, characterization of bread polymers, starch and gluten, were examined by differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA).
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  • 45
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    Journal of thermal analysis and calorimetry 47 (1996), S. 445-452 
    ISSN: 1572-8943
    Keywords: catalysts ; catalyst activity ; catalyst deactivation ; DSC ; gas reactions ; kinetics
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Gas reactions, catalyzed by solid catalysts, can be measured by DSC. In the experimental set-up an open sample pan with catalyst (powder or pellet) is placed on the sample side of the DSC sensor. The reactive gas mixture flows through the cell and reacts on the catalyst surface. The heat effect, caused by this reaction, results into a DSC signal. The calibration procedure is described for quantitative evaluation of the DSC measurements. For illustration four different reaction systems are discussed.
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  • 46
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    Journal of thermal analysis and calorimetry 47 (1996), S. 503-514 
    ISSN: 1572-8943
    Keywords: cardiac myosin ; DSC ; flexibility of myosin heads ; spin-labelling
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Conventional and saturation transfer electron paramagnetic resonance spectroscopy (EPR and ST EPR) and differential scanning calorimetry (DSC) were used to study the motional dynamics and segmental flexibility of cardiac myosins. Cardiac myosins isolated from bovine and human heart muscle were spin-labelled with isothiocyanate- or maleimide-based probe molecules at the reactive sulfhydryl sites (Cys-697 and Cys-707) of the motor domain. The maleimide probe molecules attached to human cardiac myosin rotated with an effective rotational correlation time of 33 ns which was at least eight times shorter than the rotational correlation time of the same label on skeletal myosin (260 ns). In the presence of MgADP and MgADP plus orthovanadate, flexibility changes in the multisubunit structure of myosins were detected, but this did not lead to changes of the overall rotational property of the myosin heads. Significant difference in the internal flexibility was detected on myosin samples isolated from ischemic tissue, the rotational correlation time decreased to 25 ns. DSC measurements supported the view that addition of nucleotides produced additional loosening in the multisubunit structure of cardiac myosin. It is postulated that there is an intersite communication between the nucleotide binding domain and the 20 kDa subunit where the reactive thiol sites are located.
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  • 47
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    Journal of thermal analysis and calorimetry 47 (1996), S. 765-774 
    ISSN: 1572-8943
    Keywords: calcium aluminates ; cement ; DSC
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Differential scanning calorimeter (DSC) has been used to study the dehydration characteristics of hydrated calcium aluminates such as CA, CA2 and C12A7 where C and A stand for CaO and Al2O3 respectively. Dehydration of CAH10 and C2AH8 (whereH=H2O) occur ∼ at 160–180°C and 200–280°C respectively. These two phases are unstable and ultimately get transformed to AH3 and C3AH6. Dehydration of AH3 and C3AH6 occur between 290 and 350°C and overlap at lower scanning rate. The activation energy for dehydration of the stable AH3 and C2AH6 phases has been found to be 107.16 and 35.58 kJ mol−1 respectively. The compressive strength of the hydrated calcium aluminates has been determined. The result shows that in the case of CA, almost 90% of ultimate strength has been attained in 1 day whereas in CA2, ultimate strength has been attained in 14 days and in C12A7 in 1 day. DSC results have been correlated with the rate of strength developments.
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  • 48
    ISSN: 1572-8943
    Keywords: crown-ethers (CE) ; 12-C-4 ; 15-C-5 ; DC 18-C-6anti ; DC 18-C-6syn ; DSC ; LiAlH4-CE complexes ; glass-transition ; solvates
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The thermal behaviour of complexes [Li+-EC](AlH4)− withEC=12-C-4, 15-C-5, DC 18-C-6 (cis-anti-cis andcis-syn-cis isomers) was investigated by Differential Scanning Calorimetry (DSC). These complexes were prepared as solids from benzene solutions. Pure EC and several solvated species [Li+-EC](AlH4)−·nC6H6 (EC=15-C-5, DC 18-C-6syn) were also studied. DSC has revealed various phenomena. Solid-solid transitions were observed before melting for [Li+-EC](AlH4)− withEC=12-C-4 and 15-C-5. They are probably explained by small molecular modifications strongly dependent on the thermal history of the sample. A glass-transition was found for the pure crown-ether DC 18-C-6anti, the complex [Li+-EC](A1H4)− withEC=DC-18-C-6anti and the two solvates mentioned above.
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  • 49
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    Journal of thermal analysis and calorimetry 47 (1996), S. 887-896 
    ISSN: 1572-8943
    Keywords: compensation temperature ; DSC ; fictive temperature ; poly(ethylene terephthalate) ; thermally stimulated depolarization current
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    Topics: Chemistry and Pharmacology
    Notes: Abstract This work deals with a comparison of data obtained from differential scanning calorimetry (DSC) and thermally stimulated depolarization current (TSDC) investigations. Measurements were performed on various poly(ethylene terephthalate) films: a wholly amorphous, a thermally crystallized and drawn samples. For each specimen, the TSDC complex spectra, resolved into elementary ones, led to the determination of the classical compensation temperature (T c ). The glass transition temperature (T g) and the fictive equilibrium temperature (T f) were determined by means of DSC. It appears thatT c is different fromT g and very close toT f.
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  • 50
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    Journal of thermal analysis and calorimetry 47 (1996), S. 931-939 
    ISSN: 1572-8943
    Keywords: DSC ; isothermal melt crystallization ; kinetic parameters ; Mathematica® ; new kinetic model ; poly(L-lactic acid)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A DSC study was carried out of the isothermal melt crystallization kinetics of poly(L-lactic acid), PLLA, at 110, 115, 120, 125 and 130
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  • 51
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    Plant molecular biology 30 (1996), S. 1301-1306 
    ISSN: 1573-5028
    Keywords: abscisic acid ; glycine-rich ; ribonucleoprotein ; RNA-binding protein ; RNP motif ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings. whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
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  • 52
    ISSN: 1573-5028
    Keywords: differential display ; heat shock proteins ; non-coding regions ; PCR ; reverse transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific probes from 3′ non-coding regions of different members can assist in the fast retrieval and characterization of individual members of a multigene family. We used the recent technique of differential display for the same purpose. As an example of a multigene family in plants, we selected a heat shock protein gene family, HSP16.9 from wheat, with estimated 12 members. We modified the original differential display technique for selective amplification of the 3′ non-coding regions of different wheat HSP16.9 genes by replacing the random 10-mer in the original method with a conserved HSP16.9 gene family-specific primer. Sixteen cDNA fragments from these experiments were sequenced and they represent 8 different members of a 12 member gene family. Our succes can be attributed to shorter 3′ non-coding regions that are typical of higher-plant genes and use of highly conserved gene family-specific primer in these experiments. This modified differential display technique can be of general application to other plant systems where cloning of the different members of a gene family is desired.
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  • 53
    ISSN: 1573-5028
    Keywords: male sterility ; ovary ; parthenogenetic lines ; pollen allergen ; subtractive hybridization ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To isolate genes specifically expressed at the initiation of plant embryo development we have applied a sensitive subtractive hybridization technique for three isogenic wheat lines of the so-called ‘Salmon system’ with either zygotic or autonomous embryo development. Here we present a gene sequence showing a high homology to grass pollen allergens of type II/III thought to be expressed in pollen tissue only. Surprisingly, the pollen allergen-like sequence, designated Tri a III, is also expressed in gynoecia of the sexual, male fertile wheat line ‘(aestivum)-Salmon’, whereas the two parthenogenetic and male sterile wheat lines ‘(caudata)-Salmon’ and ‘(kotschyi)-Salmon’ completely lack any Tri a III transcript. Our data suggest a positive correlation between the expression of this clone and the manifestation of male fertility. Northern and in situ hybridization analysis revealed that, in addition to its presence in pollen, Tri a III is expressed in the parenchymatous tissue of ‘(aestivum)-Salmon’ ovaries exclusively at the day of anthesis. This precise temporal and spatial expression pattern suggests a more general function of the pollen allergen-like sequence Tri a III not limited to the exhibition of allergens in pollen grains.
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  • 54
    ISSN: 1573-5028
    Keywords: cDNA ; expression ; FKBP ; PPIase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family.
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  • 55
    ISSN: 1432-203X
    Keywords: Triticum aestivum L. ; wheat ; transformation ; biolistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
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  • 56
    ISSN: 1618-2545
    Keywords: consecutive planting ; Phoma sp. ; plant growth-promotion ; potting medium ; soybean ; wheat ; zoysiagrass rhizosphere fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several isolates ofPhoma sp., certain nonsporulating fungi, as well asPenicillium andTrichoderma, all isolated from zoysiagrass rhizosphere, promoted growth of wheat and soybean under greenhouse conditions. However, the ability of these rhizosphere fungi to enhance plant growth varied with the crop tested. For example, most of the fungi effectively promoted the growth of wheat, whereas only a few fungi were effective on soybean. In consecutive plantings of wheat and soybean grown in soil previously infested with these zoysiagrass rhizosphere fungi, the growth promotion ability of the fungi was lowered. However, addition of fresh potting medium appeared to restore their growth-promotive effects. It appears that the activation of plant growth-promoting fungi in soil might depend on the availability of organic substrates to colonize, as evidenced by the promotion of plant growth.
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  • 57
    ISSN: 1573-6849
    Keywords: in situ hybridization ; intermediate wheatgrass ; translocation ; virus resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination.
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  • 58
    ISSN: 1573-5036
    Keywords: ammonium ; heterogeneity ; inflow ; localised nutrient ; mineralisation ; 15N ; nitrate ; organic residue ; proliferation ; roots ; soil ; uptake ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To obtain nutrients mineralised from organic matter in the soil, plants have to respond to its heterogeneous distribution. We measured the timing of nitrogen uptake by wheat from a localised, 15N labelled organic residue in soil, as well as the timing of changes in root length density. We calculated the rates of N uptake per unit root length (inflows) for roots growing through the residue and for the whole root system. A stimulated local inflow appeared to be the main mechanism of exploitation of the residue N during the first five days of exploitation. 8% of the N that the plants would ultimately obtain from the residue was captured in this period. Roots then proliferated in the residue. This, together with a rapidly declining N inflow, contributed to the capture, over the next seven days, of 63% of the N that the plants derived from the residue. After that time, massive root proliferation occurred in the residue, but relatively little further N was captured.
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  • 59
    ISSN: 1573-5036
    Keywords: evapotranspiration ; grain yield ; irrigation ; plant density ; water use efficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Yield of eight wheat cultivars was evaluated under rainfed and irrigated conditions in a Mediterranean environment. Variation in grain yield resulted from variation in both aboveground biomass production and in harvest index. Under rainfed compared to irrigated conditions, grain yield, biomass and days to heading were decreased, whereas harvest index was increased. Grain yield of the different cultivars under rainfed conditions correlated with that under irrigated conditions in one of the two years. Among cultivars, harvest index under rainfed and irrigated conditions were correlated in both years. Water was used more efficiently for biomass production, and equally efficiently for grain production, under irrigated compared to rainfed conditions. Under rainfed conditions, crop water use efficiency was higher for cultivars developed for rainfed environments than for those developed for high-rainfall or irrigated environments. Cultivars with low-rainfall target environments had the lowest evapotranspiration under rainfed conditions. Under rainfed conditions, differences between the cultivar groups in crop water use efficiency corresponded with trends in water use efficiency of individual plants and with the ratio of photosynthesis to transpiration, measured on plants grown in a growth room. Early in the season, water was used more efficiently for biomass production at high sowing densities than at low sowing densities. Through faster biomass production and ground cover a smaller proportion of the evapotranspired water was lost in soil evaporation and a larger proportion was transpired. However, the net effect was a greater water use in the early phases of growth and consequently a lower water availability later in the season, leading to similar yields regardless of sowing density.
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  • 60
    ISSN: 1573-5036
    Keywords: crown rot ; Fusarium ; fungal disease ; genotypes ; wheat ; zinc ; zinc efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A crown rot disease in wheat caused by the fungusFusarium graminearum Schw. Group 1 is a widespread problem in chronically Zn-deficient Australian soils. A link between crown rot and Zn deficiency was established by Sparrow and Graham (1988). This paper reports a test of a further hypothesis, that wheat genotypes more efficient at extracting zinc from low-zinc soils are more resistant to infection by this pathogen. Three wheat cultivars (Excalibur, Songlen and Durati) of differential Zn efficiency were tested at three zinc levels (0.05, 0.5 and 2.0 mg Zn kg−1 of soil) and three levels ofF. graminearum S. Group 1 inoculum (0.1 g and 0.3 g kg−1 live chaff-inoculum and control having 0.1 g kg−1 dead chaff inoculum). Six weeks after sowing dry matter production of shoots and roots was decreased byFusarium inoculation at 0.05 mg and 0.5 mg kg−1 applied Zn.Fusarium inoculum at 0.1 g was as effective as 0.3 g kg−1 for infection and decreasing dry matter. The infection at the basal part of culm decreased significantly by increasing the rate of Zn application. Excalibur, a Zn-efficient cultivar (tolerant to Zn deficiency) produced significantly more shoot and root dry matter, and showed less disease infection compared with Zn-inefficient cultivars (Durati and Songlen) at low (0.05 mg Zn kg−1 soil) and medium (0.5 mg Zn kg−1 soil) Zn fertilization rates. Higher rate of Zn fertilization (2.0 mg Zn kg−1 soil) reduced the disease level in Durati to the level of Excalibur but the disease level of Songlen was still high, indicating its high Zn requirement and or sensitivity to crown rot. The data on Zn uptake show that Excalibur, being Zn-efficient, was able to scavenge enough Zn from Zn-deficient soil, we suggest that besides sustaining growth Excalibur was able to build and maintain resistance to the pathogen; inefficient cultivars needed extra Zn fertilization to achieve performance comparable to that of Excalibur. The present study indicates that growing Zn-efficient cultivars of wheat along with judicious use of Zn fertilizer in Zn-deficient areas where crown rot is a problem may sustain wheat production by reducing the severity of the disease as well as by increasing the plant vigour through improved Zn nutrition. ei]Section editor: R Rodriques-Kalana
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  • 61
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    Euphytica 88 (1996), S. 165-174 
    ISSN: 1573-5060
    Keywords: wheat ; tritordeum ; Hordeum chilense ; Fusarium culmorum ; Septoria nodorum ; scab ; deoxynivalenol ; ergosterol ; resistance ; Triticum spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
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  • 62
    ISSN: 1573-5060
    Keywords: Aegilops markgrafii ; aneuploids ; chromosome specific library ; DOP-PCR ; microdissection ; molecular markers ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5α. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.
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  • 63
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    Euphytica 89 (1996), S. 1-10 
    ISSN: 1573-5060
    Keywords: aneuploidy ; chromosome substitutions ; height ; wheat ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The difficulties in developing inter-varietal chromosome substitution lines in wheat are reviewed. The use of genetical, cytological and molecular markers is suggested as a way of overcoming them. These difficulties and the use of markers, as well as the need to develop duplicate lines to detect background variation, are described using the development and analysis of the Cappelle-Desprez (Bezostaya 1) chromosome substitution set as an example. The effects of substituting Bezostaya 1 chromosomes on final plant height and adult-plant resistance to yellow rust are reported. The large number of aneuploids and substitution lines available in wheat provides a tremendous, international resource, which should be exploited in the future.
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  • 64
    ISSN: 1573-5060
    Keywords: Bread-making quality ; chromosome effects ; grain hardness ; SDS-sedimentation volume ; substitution lines ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The group 1 and 6 inter-varietal chromosome substitution lines of Cappelle-Desprez (Bezostaya 1) were intercrossed along with the donor and recipient varieties, Cappelle-Desprez and Bezostaya 1, to give 36 genetically different families. The analysis of the means of these families showed that variation in SDS-sedimentation volume fitted a predominantly additive model. There were no significant within or between chromosome interactions among the group 1 and 6 chromosomes. Nor was there any evidence for interactions between these chromosomes and those of the background. Significant dominance/within chromosome interactions amongst the background chromosomes were however detected. Some of the positive effects on SDS-sedimentation were associated with increased grain hardness. Chromosome effects on % grain protein were not correlated with SDS-sedimentation.
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  • 65
    ISSN: 1573-5060
    Keywords: agronomic characters ; QTL ; RFLP maps ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The advent of molecular marker systems has made it possible to develop comparative genetic maps of the genomes of related species in the Triticeae. These maps are being applied to locate and evaluate allelic and homoeoallelic variation for major genes and quantitative trait loci within wheat, and to establish the pleiotropic effects of genes. Additionally, the known locations of genes in related species can direct searches for homoeologous variation in wheat and thus facilitate the identification of new genes. Examples of such analyses include the validation of the effects of Vrn1 on chromosome 5A on flowering time in different crosses within wheat; the indication of pleiotropic effects for stress responses by the Fr1 locus on chromosome 5A; the detection of homoeologous variation for protein content on the homoeologous Group 5 chromosomes; and the detection of a new photoperiod response gene Ppd-H1 in barley from homoeology with Ppd2 of wheat.
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  • 66
    ISSN: 1573-5060
    Keywords: aneuploids ; automated DNA sequencer ; chromosomal location ; microsatellite marker ; PCR ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is described for bread wheat (Triticum aestivum). The distribution over the different chromosomes and chromosome arms appears to be random. The highest proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified unique fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, amplify fragments on homoeologous chromosomes. In some cases, more than one fragment on a single chromosome or fragments on non-homoeologous chromosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to differentiate between fragments, amplified by a single primer pair, with size differences as small as two base pairs.
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  • 67
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    Euphytica 89 (1996), S. 49-57 
    ISSN: 1573-5060
    Keywords: adaptability ; earliness per se ; photoperiod sensitivity ; vernalization sensitivity ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In order to obtain high levels of environmental adaptability in wheat varieties it is essential they flower at times appropriate to particular environmental conditions. The influence of three distinct genetic systems that together determine time of flowering is reviewed here. Vernalization genes are seen to be particularly important to winter wheats for their direct or indirect effects on winter hardiness. Vernalization genes play a minor role in determining flowering time in autumn sown winter wheats but insensitivity is essential if spring sown wheats are to flower. Day length sensitive photoperiod genes play a major role in determining flowering time and adaptability of autumn sown wheats. Insensitivity can promote yield advantages of over 35% in Southern European environments. 15% in Central Europe and offers benefits even in the UK. At present only a single allele of Ppd1 appears to have been introduced into commercial European wheat varieties. The merits of alternative Ppd1 alleles or different loci are discussed. The influence of earliness per se genes that determine flowering time independently of environmental stimuli is less well documented than the effect of photoperiod and vernalization genes. It is likely that genes on chromosomes belonging to groups 2, 3, 4, 6 and 7 may act to modify flowering time independently of environmental stimuli probably by determining numbers of vegetative and floral primordia being initiated or the rate of initiation of the primordia. Earliness per se genes appear to be widespread in European wheats and play a significant role in determining the exact time plants flower.
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  • 68
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    Euphytica 89 (1996), S. 69-75 
    ISSN: 1573-5060
    Keywords: dwarfing genes ; gene mapping ; GA insensitivity ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The improvement of lodging resistance by introducing major dwarfing genes, classified either as GA insensitive or GA sensitive, is one of the main strategies chosen by cereal breeders. In the present paper the current knowledge about the genetics, chromosomal localisation and the homoeoallelic relationships of the dwarfing genes in wheat and rye is reviewed. The confusing system of the symbolisation of the GA insensitive dwarfing genes/alleles in wheat is discussed and a nomenclature based on rules for gene symbolisation in wheat is proposed.
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  • 69
    ISSN: 1573-5060
    Keywords: Chromosomal location ; grain size ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Grain size in wheat is the most stable yield component and has a favorable effect on flour yield. To identify the chromosomes associated with the large grains of line G603-86, (grain weight over 60 mg and grain length of about 9 mm), F3 lines, extracted from F2 populations obtained from F1 monosomics of crosses between G603-86 (P1) and the monosomic set of Favorit (P2) were tested in the field. ANOVA showed significant differences among parents for grain weight and grain length, but not for grain width or the factor expressing the difference in grain form and density. Homoeologous groups had significant effects on grain weight and on all components of grain weight, while genomes were not significantly different for any of these characters. Grain weight was significantly increased by chromosomes 6D and 4A of G603-86. Grain length was significantly increased by chromosomes 4A, 4B, 2B, 3A and 1B, grain width by chromosomes 1A and 1B, and the factor form-density by chromosomes 6D and 6A. The high grain size in G603-86 results from the effects of genes located on many chromosomes which affect grain dimensions, form and density.
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  • 70
    ISSN: 1573-5060
    Keywords: chromosome substitutions ; hybrid dwarfness ; photoperiodic response ; tissue culture response ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat varieties Chinese Spring, Marquis and Thatcher and five intervarietal ‘Chinese Spring’ substitution lines for chromosomes 2B (2 lines), 2D (2 lines) and 4A*, differing from the recipient variety in alleles for hybrid dwarfing genes and/or the photoperiodic response genes were analysed for tissue culture response (TCR). It could be demonstrated that only chromosome 2B has a major effect on TCR. Allelic variation at the hybrid dwarfing loci seems to have no effect on tissue culture performance, even in the combination D1D2D3 which gives the grass dwarf phenotype. Also the allelic constitution at the Ppd loci, gave no indication for a direct major effect of those alleles, however there seems to exist genetical factors for TCR on the homoeologous group 2 chromosomes which may be closely linked to the Ppd loci.
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  • 71
    ISSN: 1573-5060
    Keywords: Triticum miguschovae ; wheat ; leaf rust resistance ; transfer lines ; monosomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Triticum miguschovae, a genome addition synthetic, was used as a source for transfer of leaf rust (Puccinia recondita tritici) resistance to common wheat. This synthetic, developed from two wild species Triticum militinae and Aegilops squarrosa, proves a valuable donor of the genes for leaf rust resistance. Leaf rust resistance was transferred from T. miguschovae by both dominant and recessive genes. Stable lines phenotypically similar to their recurrent parents Kavkaz and Bezostaya 1 but differing from them in a high level of leaf rust resistance were obtained. The genes for resistance in 3 selected lines differed from each other and from the known effective genes Lr9, Lr19, and Lr24. The resistance of one of them (line 1229) is controlled by two complementary interacting genes located on chromosome 7B and 1D was revealed by monosomic analysis.
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  • 72
    ISSN: 1573-5060
    Keywords: Aneuploidy ; dwarfing genes ; Triticum aestivum ; preferential transmission ; Aegilops sharonensis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4S l from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4S l L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.
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  • 73
    ISSN: 1573-5060
    Keywords: C-banding ; FISH ; heterochromatin ; in situ hybridization ; rye ; Secale cereale ; Secale montanum ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The sequential combination of C-banding and in situ hybridization techniques applied in this or in a reverse order, are used to recognize targeted chromosomal regions in cereals. Both methods are described whereby standard chromosome squash preparations are followed by: i) C-banding technique using Leishman stain and a slightly modified in situ hybridization technique using biotin-labeled DNA probes, or ii) fluorescence in situ hybridization technique and C-banding. Both approaches have been successfully used onto mitotic chromosomes of rye and wheat resulting suitable for both their identification and detection of targeted sites.
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  • 74
    ISSN: 1573-5060
    Keywords: alien introduction ; chromosome pairing ; fluorescent in situ hybridization ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fluorescent in situ hybridization (FISH) of DNA to plant chromosomes has proved to be a powerful cytogenetic tool. The value of fluorescent in situ hybridization of total genomic DNA (GISH) of related species is demonstrated in the determination of wheat/alien chromosome pairing in hybrids. Its use for assessing the relative merits of the various genes that affect chromosome pairing is also shown. The ability of GISH to identify the presence in wheat of whole alien chromosomes or alien chromosome segments is illustrated. The potential of FISH for detecting repeated DNA sequences, low copy sequences and single copy genes is discussed.
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  • 75
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    Euphytica 89 (1996), S. 215-221 
    ISSN: 1573-5060
    Keywords: agronomic characteristics ; breeding trends ; quality ; year of release ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat breeding efforts for a half century in Japan were investigated by using of 129 varieties registered in MAFF from 1929 to 1984 in the view point of case history for three years. Varieties released in each breeding station are classified apparently by growth habit associated closely with duration of the cold requirement. Heading date is earlier in varieties released in southwest breeding stations (southern varieties) than in varieties released in northern breeding stations (northern varieties). Culm length is higher and the pre-harvest sprouting is more sensitive in northern varieties. In quality characters, northern varieties has the higher milling rate, lower apparent amylose content and larger particle size of flour. In varieties released in the Tohoku district, trend of breeding direction with time is smaller ear numbers and larger 1,000-grain weight and greater resistance to powdery mildew. In varieties released in the Kyushu district, a significant correlation with released year was clearly observed with regard to early maturity, short culm length, less grain crude protein content, less apparent amylose content and higher milling rate on breeding advancement.
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  • 76
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; stripe rust ; epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten spring bread wheat cultivars with a susceptible seedling reaction to race 14E14 of yellow rust were tested at three locations to assess the level and stability of quantitative resistance. Quantitative resistance was expressed in terms of disease severity (DS), area under the disease progress curve (AUDPC), apparent infection rate (r), infection type (IT) and infection density. Large genotypic differences were observed for all variables measured. Morocco was the most susceptible cultivar. Based on its high infection type (IT=9) throughout the epidemics, it most likely does not possess any resistance. All other cultivars carry quantitative resistance. The levels ranged from very low (Taichung 23) to very high (Parula). Resistance levels were lower in Quito, Ecuador than at the other locations. Most likely, the lower temperatures in Quito resulted in a reduced expression of quantitative resistance to yellow rust and to obtain the same protection as at the other two locations, more resistance genes are needed. Therefore, to accumulate genes for quantitative resistance, Quito is considered to be the better location. Though significant cultivar-location interactions were detected, they were small compared to the cultivar and location effect. Therefore, they are considered of little importance and it is concluded that quantitative resistance is a stable trait, in the sense that cultivar rankings are hardly affected by environment. The contribution of infection growth to the development of yellow rust was demonstrated. Between 29 and 66% of the increase in disease severity could be contributed to growth of infections. These figures are probably an underestimation of the real contribution as new infections are very small, thus reducing the average size of infections and their contribution to the increase of disease severity.
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  • 77
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    Euphytica 91 (1996), S. 31-35 
    ISSN: 1573-5060
    Keywords: aluminum ; linkage map ; marker ; RFLP mapping ; Triticum aestivum ; Triticum turgidum ; wheat ; durum wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The tolerance of aluminum (Al) of disomic substitution lines having the chromosomes of the D genome of Triticum aestivum L. cv. Chinese Spring individually substituted for their homoeologues in T. turgidum L. cv. Langdon was investigated by the hematoxylin method. The disomic substitution lines involving chromosome 4D were more Al tolerant than Langdon. The tolerance was found to be controlled by a single dominant gene, designated Alt2, that is in the proximal region of the long arm of chromosome 4D. The locus was mapped relative to molecular markers utilizing a population of recombinant chromosomes from homoeologous recombination between Chinese Spring chromosome 4D and T. turgidum chromosome 4B. Comparison of the location of Alt2 in this map with a consensus map of chromosomes 4B and 4D based on homologous recombination indicated that Alt2 is in a vicinity of a 4 cM interval delineated by markers Xpsr914 and Xpsr1051. The Alt2 locus is distal to marker Xpsr39 and proximal to XksuC2. The Altw locus is also proximal to the Knal locus on chromosome 4D that controls K+/Na+ selectivity and salt tolerance. In two lines, Alt 2 and Knal were transferred on a single 4D segment into the long arm of T. turgidum chromosome 4B.
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  • 78
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    Euphytica 91 (1996), S. 127-140 
    ISSN: 1573-5060
    Keywords: In vitro selection ; salt tolerance ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Embryogenic calli isolated from immature embryos of four wheat cultivars were subjected to three in vitro selection methods for salt tolerance. The effect of NaCl on the selected and unselected cell lines has been investigated. The results indicated that the relative growth rate of callus decreased as the concentration of NaCl increased in both callus lines. The selected callus line gave a higher growth weight in the presence of NaCl in the medium and was highly significant as compared with unselected callus line across medium protocols in all wheat cultivars. The dry weight of both kinds of callus lines of all wheat cultivars increased markedly with increasing NaCl concentration in most cases. The Na+ and Cl- contents of both callus lines were increased with increasing salinity levels while K+ content was decreased. The selected callus line of each cultivar at the same salinity level produced significant amounts of Na+, K+ and Cl- higher than the unselected callus line in most salinity levels. However, the unselected callus lines of the cultivars Giza-157 and Sakha-90 at the same salinity level produced significant amounts of K+ higher than the selected callus line in most salinity levels. The proline content of both kinds of callus lines for all wheat cultivars was increased with increasing salinity level. However, the selected callus line gave a significantly higher proline content than the unselected callus line in all wheat cultivars at the same Salinity level. Results from the in vitro selection for NaCl tolerance showed that the stepwise method of increasing NaCl in the medium was more effective for plant regeneration than other methods.
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  • 79
    ISSN: 1573-5060
    Keywords: adaptation ; intrinsic earliness ; Triticum aestivum ; vernalization ; wheat ; Syria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study sought to identify factors that influence wheat development in the transitional wheat growing zone of northern Syria. Three development factors were studied, intrinsic earliness, and responses to vernalization and to photoperiod. Two sets of wheat were studied, each composed of lines with differing combinations of development factors. Set 1 comprised 20 parental and breeding lines utilized by the CIMMYT/ICARDA facultative and winter wheat breeding program based at Tel Hadya. Set 2 comprised 19 parental and breeding lines utilized by an Australian winter wheat breeding program based at Temora. Field development was recorded in greatest detail at one site. Tel Hadya, using the state of differentiation of the apex of the main tiller of sampled plants. To extend findings, development was also recorded as the time from sowing to ear emergence for later sowings of wheat at Tel Hadya, and in sowings at four other regional sites. The significance of each development factor was tested in multiple regressions that predicted either stage of apical development at Tel Hadya, or time to ear emergence in all trials. It was found that intrinsic earliness was the major factor associated with development, in both sets of wheat. Response to photoperiod had a much smaller and less consistent effect. Response to vernalization had least effect on development, possibly because low temperature in winter delayed development for a longer period than was required to fully vernalize winter wheats. Our results suggested it may not be directly relevant whether spring or winter wheats are grown in the transitional zone of northern Syria. The desired phenotype for the region, of slow development prior to double ridge, then fast development to ear emergence, cannot be simply achieved from combinations of the three development factors. Selection for improved adaptation to the region must continue to rely on direct field observations.
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  • 80
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    Euphytica 92 (1996), S. 147-153 
    ISSN: 1573-5060
    Keywords: breeding methodology ; drought patterns ; input-efficiency ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary About 32% of the 99 million ha wheat grown in developing countries experiences varying levels of drought stress. Three major drought types have been identified: Late drought (LD) is common in the Mediterranean region, early drought (ED) is found in Latin America and wheat is produced on residual soil moisture (RM) in the Indian subcontinent and part of Australia. Until 1983, CIMMYT selected all germplasm under near optimum conditions for its yield potential and tested only advanced lines under drought. In spite of many critics, this approach proved to be successful, since in the mid 80's CIMMYT germplasm was grown on 45% of the wheat area in LC with annual rainfall from 300–500 mm and on 21% in areas with less than 300 mm. Since 1983, CIMMYT's drought breeding methodology is to alternate segregating populations between drought stressed and fully irrigated conditions (FI) and to test advanced lines under a line source irrigation system. To compare the efficiency of these approach, yield of four, mostly leading varieties, from each of the regions with LD, ED, RM, and FI and twelve recent CIMMYT cultivars selected for high yield under FI and RM conditions (ALT) were compared under four different moisture regimes (FI, LD, ED, and RM) in 89–90 and 90–91 in Yaqui Valley, Mexico. Genotypic correlation between yield and days to flowering, days to maturity, height, grains m-2, TKW, test weight and grain fill period were calculated. Mean grain yield of the four best lines in the ALT group was highest under all moisture stress regimes, followed by the FI-group. However, the highest yielding cultivar within each moisture regime was from the FI-group under FI, from the LD-group under LD, and from the ALT-group under ED and RM conditions. Estimates for genetic advance suggest that FI is the best environment for increasing grain yield even in all three drought environments. This indicates that yield potential per se is beneficial also in drought environments. The highest yield in drought environments was realized by the CIM cultivars selected under FI and RM. Simultaneous evaluation of the germplasm under near optimum conditions, to utilize high heritabilities and identify lines with high yield potential, and under stress conditions to preserve alleles for drought tolerance seem at present the best strategy.
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  • 81
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    Euphytica 92 (1996), S. 221-224 
    ISSN: 1573-5060
    Keywords: hybrid lines ; nitrogen efficiency ; variation ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The new European Common Agricultural Policy and environmental considerations are certainly to change agricultural practices toward low input cultivation systems. Nitrogen is one of the main inputs of winter wheat in northern France and it contributes highly to phreatic water pollution. A research programme has then been set up in order to study whether it is possible to breed for winter wheat cultivars using more efficiently N fertilisers. Less nitrogen would be applied, decreasing pollution risks and operational costs. It has been shown that a large variation exists for N related traits and for the resistance against N deficiency. On the one hand the cv Arche is very resistant to N deficiency, its yield on low N conditions (with no N fertiliser) is on average 89% of its yield on high N conditions (with a high N application). On the other hand, cv Récital is very susceptible to N deficiency as this same percentage is only 61%. A study on 10 hybrids showed that heterosis for grain yield was higher at low N level than at high N level. This was due to a higher number of grains per m2.
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  • 82
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    Plant and soil 178 (1996), S. 223-230 
    ISSN: 1573-5036
    Keywords: barley ; cultivar ; acquisition efficiency ; rhizosphere ; root hairs ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To assess the extent of variation in phosphorus acquisition efficiency of some winter wheat (Triticum aestivum L.), winter and spring barley (Hordeum vulgare L.) genotypes, depletion of inorganic phosphorus (P) extractable with 0.5 M NaHCO3 (NaHCO3-Pi) from the rhizosphere soil was studied. Nutrients supply, rhizosphere soil pH and soil water content was kept equal for all the genotypes with the aim to reduce the confounding variation due to these factors. The experimental set up implied that no difference in the relative growth rates, nitrogen, potassium and calcium content of shoot dry matter occurred among the genotypes. The winter wheat, winter barley and spring barley genotypes differed significantly (p〉0.05) in their efficiency to acquire NaHCO3-Pi from the rhizosphere soil. The efficiency of the winter wheat genotypes to acquire NaHCO3-Pi from rhizosphere soil ranked Kraka 〉 Gawain 〉 Foreman 〉 Sleipner = Obelisk 〉 Kosack 〉 Pepital 〉 Arum. Winter wheat genotypes differed in extent of P depletion profiles in the rhizosphere, indicating variation in root hair length. The winter barley and spring barley genotypes also showed significant differences in their P depletion profiles near roots. The efficiency of the winter barley genotypes to acquire soil P in the rhizosphere ranked Hamu 〉 Frost 〉 Marinka 〉 Astrid 〉 Clarine = Angora. The efficiency of spring barley genotypes to acquire NaHCO3-Pi in the rhizosphere ranked Canut 〉 Etna ≅ Riga 〉 Digger 〉 Peel 〉 Semal 〉 Alexis. The rhizosphere pH remained unchanged, suggesting that additional mechanisms such as root hair formation and root exudates play a significant role in causing variation in P acquisition among the genotypes.
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  • 83
    ISSN: 1573-5036
    Keywords: genotypes ; phytosiderophores ; wheat ; zinc deficiency ; zinc efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of the zinc (Zn) nutritional status on the rate of phytosiderophore release was studied in nutrient solution over 20 days in four bread wheat (Triticum aestivum cvs. Kiraç-66, Gerek-79, Aroona and Kirkpinar) and four durum wheat (Triticum durum cvs. BDMM-19, Kunduru-1149, Kiziltan-91 and Durati) genotypes differing in Zn efficiency. Visual Zn deficiency symptoms, such as whitish-brown necrosis on leaves and reduction in plant height appeared first and more severe in Zn-inefficient durum wheat genotypes Kiziltan-91, Durati and Kunduru-1149. Compared to the bread wheat genotypes, all durum wheat genotypes were more sensitive to Zn deficiency. BDMM-19 was the least affected durum wheat genotype. Among the bread wheat genotypes, Kirkpinar was the most sensitive genotype. In all genotypes well supplied with Zn, the rate of phytosiderophore release was very low and did not exceed 1 μmol 32 plants-1 3h-1, or 0.5 μmol g-1 root dry wt 3h-1. However, under Zn deficiency, with the onset of visual Zn deficiency symptoms, the release of phytosiderophores was enhanced in bread wheat genotypes up to 7.5 μmol 32 plants-1 3h-1, or 9 μmol g-1 root dry wt 3h-1, particularly in Zn-efficient Kiraç-66, Gerek-79 and Aroona. In contrast to bread wheat genotypes, phytosiderophore release in Zn-deficient durum wheat genotypes remained at a very low rate. Among the durum wheat genotypes BDMM-19 had highest rate of phytosiderophore release. HPLC analysis of root exudates showed that 2′-deoxymugineic acid (DMA) is the dominating phytosiderophore released from roots of Zn-efficient genotypes. In root extracts concentration of DMA was also much higher in Zn-efficient than in inefficient genotypes. The results demonstrate that enhanced synthesis and release of phytosiderophores at deficient Zn supply is involved in Zn efficiency in wheat genotypes. It is suggested that the expression of Zn efficiency mechanism is causally related to phytosiderophore-mediated enhanced mobilization of Zn from sparingly soluble Zn pools and from adsorption sites, both in the rhizosphere and plants.
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  • 84
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; gene action ; heritability ; wheat ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Estimates of gene actions were obtained for five in vitro traits of immature wheat (Triticum aestivum L.) embryo cultures from a cross of two wheat cultivars and the resulting reciprocal, F1, F2 and backcross populations. The contribution of additive gene effects to in vitro traits was not as important as the dominance gene effects. Epistatic gene effects were relatively more important than either additive or dominance gene effects. Of the individual types of digenic epistatic effects, the dominance x dominance estimates were relatively larger in magnitude for all in vitro culture traits measured. The maternal effect played a minor role in the inheritance of the in vitro studied traits since the difference among the reciprocal values was not significant. It is shown from the generation mean method that epistasis played a major role in the inheritance of most of the traits under study. The negative values of additive and dominance genetic variance were estimates of zero. Heritability estimates, in broad sense, were relatively high for the in vitro studied traits. In some cases, heritability estimates in broad and narrow senses are almost equal since the estimation of dominance genetic variance led to negative values. According to the results of the gene effects, dominance and epistasis were important for the shoot formation trait. Selection would be effective among the isolated genotypes on individual basis.
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  • 85
    ISSN: 1573-5060
    Keywords: Secale cereale ; Triticum aestivum ; yield components ; 1AL/1RS translocation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of the 1AL/1RS chromosome translocation on grain yield and other agronomic characteristics of 85 random F2-derived F6 bulks from three 1AL 1RS × 1A bread wheat crosses was determined under optimum and reduced irrigation conditions at CIANO, Yaqui Valley, Sonora, Mexico, during the 1991–1992 and 1992–1993 crop production cycles. Harvest plots of 5.0 m2 were arranged in an alpha lattice design with three replications. The 1AL/1RS translocation increased grain yield, above-ground biomass, spikes/m2, and test weight under both irrigated and dryland conditions. Homozygous chromosome 1A lines, on the other hand, possessed longer spikes with more grains. The 1AL/1RS cultivars had an advantage in 1000-grain weight, which was detected only under optimum irrigation. The translocation lines showed later maturity and longer grainfilling period than the 1A genotypes under one irrigation treatment. A significant relationship between grain yield and test weight was detected only among the 1AL/1RS genotypes, indicating that they possess heavier and plumper grains than the 1A genotypes. These results encourage the continued use of the 1AL/1RS translocation in wheat improvement.
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  • 86
    ISSN: 1573-5060
    Keywords: embryogenesis ; wheat ; maize ; Triticum aestivum ; Zea mays ; haploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Embryogenesis was analyzed in wheat × maize hybrids using paraffin sectioning. Embryogenesis in wheat × maize hybrids is different from that in self-pollinated wheat plants. Development of the embryo is not accompanied by the formation of an endosperm. The endosperm nuclei remain free in the cytoplasm, fail to advance into the cellular stage, and degenerate at a later time. The antipodal cells quickly degenerate in the fertilized ovaries of wheat × maize hybrids similar to self-pollinated ovaries. The antipodal cells remain normal in unpollinated ovaries. The pre-embryo will abort if it is allowed to develop on the plant, because of a nutritional shortage in the absence of an endosperm. Therefore, embryo rescue is necessary for haploid production from a wheat × maize hybrids. Haploid polyembryos were obtained from spikelet culture of wheat × maize hybrids. The formation of polyembryos is due to the cleavage of the pre-embryo and the effect of 2,4-D. The frequency of haploid embryo production and plant regeneration is affected significantly by maize genotypes, but not by wheat genotypes. The concentration of 2,4-D affects only the size of the embryo.
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  • 87
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    Euphytica 92 (1996), S. 95-103 
    ISSN: 1573-5060
    Keywords: rusts ; virulence genes ; wheat ; resistance genes ; Czech Republic ; Slovak Republic ; Triticum aestivum ; Thalictrum species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes of resistance genes in the grown cultivars. Unnecessary genes for virulence had no negative effect on the fitness of the pathogen. All tested samples of aeciospores from barberries attacked rye, not wheat. None of Thalictrum species occurring in the Czech and Slovak Republics was found to host wheat leaf rust. However, the sexual stage of wheat stem rust and wheat leaf rust could be induced on Berberis vulgaris and Thalictrum speciosissimum, respectively. General epidemiological conclusions are drawn from the results and experience of the last 35 years.
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  • 88
    ISSN: 1573-5109
    Keywords: genetic diversity ; HMW glutenin subunits ; landraces ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sixty hexaploid wheat landraces collected from five regions of Pakistan were assessed for genetic variability in terms of high molecular weight (HMW) glutenin subunits as revealed by SDS-PAGE. The germplasm appeared to be diverse and unique on the basis of HMW glutenin subunit compositions. Out of 24 alleles detected at all the Glu-1 loci, four belonged to Glu-A1, 12 to Glu-B1 and eight to Glu-D1 locus. The number of novel HMW glutenin subunits detected were 1, 4 and 6 at the three loci (Glu-A1, Glu-B1, Glu-D1), respectively. The frequency distribution patterns of 24 allelic variants detected at the three Glu-1 loci in 1080 samples analysed for 60 accessions were determined both on the basis of individual accessions and on the basis of regions (accessions pooled across the regions). One allele (“null”) at the Glu-A1 locus, three alleles (17+18, 7+8, 14) at the Glu-B1 locus and, two alleles (2+12 and 2**+12′) at the Glu-D1 locus were found most frequently distributed in the 60 populations. Maximum variation was observed in the Baluchistan and Gilgit regions of Pakistan in terms of distribution of novel Glu-1 alleles. A higher gene diversity was observed between the populations as compared to the gene diversity within the populations while, a reverse pattern of gene diversity was observed when populations were pooled across the regions (higher within the regions than between the regions). A data base has been generated in this study which could be expanded and usefully exploited for cultivar development or management of gene bank accessions.
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  • 89
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    Environmental biology of fishes 45 (1996), S. 219-235 
    ISSN: 1573-5133
    Keywords: Ecology ; Behaviour ; Evolution ; Cichlids ; Fisheries ; Conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis Ecological conditions in tropical lacustrine systems are considered by focusing on the evolution, maintenance, exploitation and vulnerability of fish communities in the African Great Lakes. The exceptionally high biodiversities in the littoral/sublittoral zones of the very ancient, deep, clear, permanently stratified rift lakes Tanganyika and Malawi, are contrasted with the simpler systems in their pelagic zones, also with biodiversity in the much younger, shallower Victoria, the world's largest tropical lake.
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  • 90
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    Genetica 97 (1996), S. 243-254 
    ISSN: 1573-6857
    Keywords: durable resistance ; induced resistance ; leaf rust ; rust resistance ; stem rust ; stripe rust ; wheat ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It is known that few wheat cultivars maintain their resistance to rust diseases for a long period of time, particularly when crop populations become genetically more uniform. A number of genetically diverse, so far unexploited, sources of rust resistance in the natural as well as mutagenized population of wheat cultivars were identified. Several of these genes were placed in agronomically superior well-adapted backgrounds so that they could be used as pre-breeding stocks for introducing genetic diversity for resistance in a crop population. Some of these stocks when employed as parents in several cross combinations in a breeding programme have generated a number of promising cultivars with diversity for resistance. Many presently grown wheats in India, near-isogenic lines each with Lr14b, Lr14ab, Lr30 and certain international cultivars were identified as possessing diverse sources of adult plant resistance (APR) to leaf rust. Prolonged leaf rust resistance in some of the Indian cultivars was attributed to the likely presence of Lr34 either alone or in combination with other APR components. Tests of allelism carried out in certain cultivars that continue to show adequate levels of field resistance confirm the presence of Lr34, which explains the role that this gene has played in imparting durability for resistance to leaf rust. Also, Lr34 in combination with other APR components increases the levels of resistance, which suggests that combination of certain APR components should be another important strategy for breeding cultivars conferring durable and adequate levels of resistance. A new adult plant leaf rust resistance source that seems to be associated with durability in ‘Arjun’ has been postulated. Likewise, cultivars possessing Sr2 in combination with certain other specific genes have maintained resistance to stem rust. Further, non-specific resistances that were transferred across widely different genotypes into two of the popular Indian wheats provided easily usable materials to the national breeding programmes for imparting durable resistance to stripe rust.
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  • 91
    ISSN: 1573-0867
    Keywords: China ; Loess Plateau ; maize ; 15N ; Nitrogen ; urea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field trials were carried out to study the fate of15N-labelled urea applied to summer maize and winter wheat in loess soils in Shaanxi Province, north-west China. In the maize experiment, nitrogen was applied at rates of 0 or 210 kg N ha−1, either as a surface application, mixed uniformly with the top 0.15 m of soil, or placed in holes 0.1 m deep adjacent to each plant and then covered with soil. In the wheat experiment, nitrogen was applied at rates of 0, 75 or 150 kg N ha−1, either to the surface, or incorporated by mixing with the top 0.15 m, or placed in a band at 0.15 m depth. Measurements were made of crop N uptake, residual fertilizer N and soil mineral N. The total above-ground dry matter yield of maize varied between 7.6 and 11.9 t ha−1. The crop recovery of fertilizer N following point placement was 25% of that applied, which was higher than that from the surface application (18%) or incorporation by mixing (18%). The total grain yield of wheat varied between 4.3 and 4.7 t ha−1. In the surface applications, the recovery of fertilizer-derived nitrogen (25%) was considerably lower than that from the mixing treatments and banded placements (33 and 36%). The fertilizer N application rate had a significant effect on grain and total dry matter yield, as well as on total N uptake and grain N contents. The main mechanism for loss of N appeared to be by ammonia volatilization, rather than leaching. High mineral N concentrations remained in the soil at harvest, following both crops, demonstrating a potential for significant reductions in N application rates without associated loss in yield.
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  • 92
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    Plant growth regulation 18 (1996), S. 201-206 
    ISSN: 1573-5087
    Keywords: paclobutrazol ; protection ; stress ; waterlogged ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (Triticum aestivum L.) seeds were imbibed for 18 h in 40 mM KCl solution containing either distilled water or 100 mg l−1 paclobutrazol (PBZ, a triazole) and then air dried. Seeds were germinated in a greenhouse and one set of seedlings was grown under non-stressed conditions. Another three sets were stressed by waterlogging at three different stages when the PBZ-treated seedlings were 3, 6, or 10 cm above the soil surface. During waterlogging, the soil was saturated by maintaining the water level 3 cm above the soil surface. For waterlogged plants, the first visible symptoms of chlorosis and wilting occurred 2 to 4 days after stress was initiated. There was a concomitant decrease in chlorophyll (a+b) and carotenoid levels. A decrease in fluorescence and increase in leakiness indicated photoinhibitory damage, lower photosynthetic efficiency, and a loss of membrane integrity. These symptoms of stress induced by waterlogging at different stages were reduced by PBZ.
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  • 93
    ISSN: 1573-5060
    Keywords: gramineae ; polyembryony ; somatic embryogenesis ; Triticum aestivum ; wheat ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to developing spikes, it was found that three cultivars showed a different ability to produce polyembryos (Thatcher 20.19%, Chris 7.06%, Dollar 0%). This behaviour was related to their capacity to form somatic embryos. Diploid immature embryos cultured in vitro after 2,4-D treatment, gave a higher frequency of embryogenic callus in Thatcher and Chris than in Dollar. As the common factor in both experiments was the 2,4-D treatment we propose that the three cultivars showed a differential sensitivity to 2,4-D.
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  • 94
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    Euphytica 92 (1996), S. 175-183 
    ISSN: 1573-5060
    Keywords: adaptation ; durable resistance ; N-use efficiency ; stability ; wheat ; yield potential ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat area in developing countries, including China, is around 100 million ha. To address the needs of these very diverse wheat growing areas, CIMMYT has defined 12 wheat mega-environments (ME). A ME is defined as broad, not necessarily continuous often transcontinental area with similar biotic and abiotic stresses, cropping systems and consumer preferences. The factors describing each ME are presented. CIMMYT's breeding methodology is centered around the development of widely adapted germplasm with high and stable yield across a wide range of environments. Segregating populations are alternating screened in two diverse environments in Mexico. One key requirement is that all germplasm is tested under near optimum conditions for its yield potential. The second one is multi-locational testing of advanced lines at sites that represent a given ME (key locations) and careful screening of germplasm for tolerance to abiotic and biotic stresses specific to that environment. This methodology has permitted the pyramiding of a large number of multiple resistance genes for use against a wide spectrum of diseases and tolerance to abiotic stresses within each ME. In addition, the widespread testing of lines allows the identification of traits which are beneficial in several environments. Data from international nurseries are used to further delineate environments within an ME. This approach has proven to be successful since around 70% of the spring wheat area in developing countries (excluding China) is planted to varieties derived directly or indirectly from CIMMYT germplasm. The performance of the bread wheat cultivar Pastor in international trials is given as an example for a wide adaptation.
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  • 95
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    Springer
    Euphytica 88 (1996), S. 69-76 
    ISSN: 1573-5060
    Keywords: dwarfing genes ; late maturity α-amylase ; gibberellic acid insensitivity ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two wheat cultivars, Spica and Lerma 52, which consistently produce high levels of α-amylase during the later stages of grain development (late maturity α-amylase), were crossed with a set of four near-isogenic lines carrying the tall (rht) allele or one of the dwarfing genes Rht1, Rht2 or Rht3 (GA-insensitive alleles). The F1 and F2 populations were developed and analysed for grain α-amylase and plant height. The Rht3 gene exhibited the strongest influence on plant height and strongly inhibited new α-amylase synthesis during the later part of grain ripening. By comparison, Rht1 and Rht2 had a less pronounced effect but still significantly reduced the expression of late maturity α-amylase. These observations suggest that gibberellic acid is involved either directly or indirectly in this phenomenon. The implications of the effect of dwarfing genes on expression of late maturity α-amylase are discussed in relation to cultivar improvement and to the identification and control of high α-amylase germplasm.
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  • 96
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 88 (1996), S. 61-67 
    ISSN: 1573-5060
    Keywords: inheritance ; late maturity α-amylase ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two wheat cultivars that consistently show high levels of grain α-amylase at harvest ripeness, in the absence of preharvest sprouting, were crossed with a control, low α-amylase cultivar, and F1, F2 and BC1 populations were developed. Grain of these populations was analysed for α-amylase activity at harvest ripeness. Distribution and segregation patterns were consistent with control at a single locus with high α-amylase the recessive allele. This mode of inheritance would make it extremely difficult to differentiate homozygous low α-amylase lines from heterozygotes (low α-amylase phenotype but carriers of high α-amylase) and has important implications for wheat breeders. High α-amylase, termed late maturity α-amylase, was not linked with the awned inhibitor gene, B2, located on the long arm of chromsome 6B.
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  • 97
    ISSN: 1573-5060
    Keywords: crossability ; kr1 gene ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The recessive of crossability allele kr1 was transferred from the spring wheat variety Chinese Spring (CS) into the winter wheat variety Martonvásári 9 (Mv9) by backcrossing the Mv9 × CS hybrids with Mv9. The Mv9 variety possesses dominant Kr1 alleles and is heterogeneous at the kr2 locus, so that some individual plants carry recessive kr2 alleles. The selection of plants possessing the recessive kr alleles from the (Mv9 × CS)Mv9 BC1 generation was carried out according to the seed set achieved when pollinated with rye (Secale cereale L. cv. Mercator). The partial dominance of the Kr alleles made it possible to differentiate between plants heterozygous at the Kr1 locus and Kr1Kr1 homozygous dominant plants. Two selfed consecutive progenies were tested by pollination with rye to select the homozygous recessive kr1kr1kr2kr2 plants and to check the result of the selection after each backcross. As a result of three backcrosses with Mv9 and two selfings after each backcross the selected progenies had 61.6% seed set with rye tested on sixty individual plants. These data confirm that after the third backcross the selected Mv9 kr1 line carries necessive crossability alleles Kr1 and Kr2, but the genotype is 93.75% Mv9.
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  • 98
    ISSN: 1573-5060
    Keywords: electrophoresis ; gluten strength ; prolamins ; Triticum aestivum ; variation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Allelic variation of prolamin loci was examined in the F2 from crosses between the hexaploid wheat varieties: ‘Cajeme 71’, ‘Yécora 70’, ‘Ablaca’, ‘Anza’, ‘Pané 247’ and ‘Axona’. Different allelic blocks for gliadins and LMW glutenin subunits were determined in Gli-1, Gli-2 and Glu-3 loci. A percentage of recombination of 1.5 ± 0.3 was determined between Gli-A1 and Glu-3 in the F2 progeny of ‘Yécora 70’ x ‘Axona’. A significant positive association was found between gluten strength, measured by SDS-sedimentation volume, and the prolamins coded by ‘Anza’ Gli-D1/Glu-D3 loci and ‘Yécora 70’ Gli-A1/Glu-A3 loci. Interactions between non homeologous loci Glu-1 and Gli-1/Glu-3 were also found.
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  • 99
    ISSN: 1573-5060
    Keywords: chromosomal arm location ; chromosome location ; ditelosomic analysis ; monosomic analysis ; Septoria glume blotch ; Stagonospora nodorum ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Septoria glume blotch, caused by Stagonospora nodorum, is an important disease of wheat (Triticum aestivum). Separate genetic mechanisms were found to control flag leaf and spike resistance. Genes for resistance to S. nodorum were located on different chromosomes in the few wheat cultivars studied. These studies only partially agree on the chromosome locations of gene in wheat for resistance to S. nodorum, and chromosomal arm locations of such genes are not known. The objectives of this study were to determine the chromosome and chromosomal arm locations of genes that significantly influence resistance to S. nodorum in wheat cultivar Cotipora. Monosomic analysis showed that flag leaf resistance was controlled by genes on chromosomes 3A, 4A, and 3B whereas the spike resistance was controlled by genes on chromosomes 3A, 4A, 7A, and 3B (P=0.01). Additionally, genes on chromosomes 6B and 5A influenced the susceptibility of the flag leaf and spike reactions, respectively (P=0.01). Telocentric analysis showed that genes on both arms of chromosome 3A, and the long arms of chromosomes 4A and 3B were involved in the flag leaf resistance whereas genes on both arms of chromosome 4A, the short arm of chromosome 3A, and the long arm of chromosome 3B conferred spike resistance.
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  • 100
    ISSN: 1573-5060
    Keywords: Fusarium resistance ; Fusarium spp. ; wheat ; Triticum aestivum ; culture filtrate ; in vitro selection ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Calli of resistant, intermediary and susceptible wheat (Triticum aestivum L.) varieties were selected using culture filtrates of Fusarium graminearum and F. culmorum and the regenerants were evaluated for resistance up to R3. Czapek-Dox broth medium was inoculated with mycelia of Fusarium isolates and incubated for 2–6 weeks. Filtrates were added to MS callus growing medium, then 5 weeks-old calli were transferred onto this medium (MST) for 4–5 weeks. MST containing 30% filtrate was found to be suitable for selection. Resistant calli were transferred again to fresh MST for further two selection cycles. The surviving calli produced less fertile regenerated lines (R0) than the non-selected ones. Among 18 R1 lines tested for Fusarium-resistance in the seedling stage by artificial inoculation in the greenhouse, two (11.1%) were significantly more resistant, one (5.6%) was more susceptible than the original cultivar and the rest (83.3%) behaved similarly to the donor plants. Among unselected R3 lines of three varieties, practically the same number of resistant plants were found as among the related selected ones. When the R3 selfed generations obtained through double-layer and culture filtrate selection techniques were tested for Fusarium-resistance, 35.7% of the lines were found to be more resistant than the original cultivars, none was more susceptible and 64.3% had a reaction similar to that of the source materials. Thus, inheritance of the disease reaction was not stable in all cases. Success of in vitro selection for Fusarium-resistance depended also on the genotype, and toxin analysis showed that although being effective, the selective media contained deoxynivalenol only exceptionally. In selecting wheat for Fusarium-resistance in vitro, the culture filtrate technique proved better than the double-layer procedure.
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