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  • Articles  (134)
  • Articles: DFG German National Licenses  (134)
  • Blackwell Verlag GmbH  (134)
  • 2005-2009  (134)
  • 1
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Leaf area, length and width affect the photosynthetic capability of a plant and so increasing the photosynthetic rate per unit leaf area may improve seed yield in soybean. In this study, simple sequence repeat (SSR) markers were used to identify the genomic regions significantly associated with the quantitative trait locus (QTL) that controls length, width and the length/width ratio of the terminal and lateral leaflet in two segregating F2:10 recombinant inbred line (RIL) populations, ‘Keounolkong’ × ‘Shinpaldalkong’ (K/S) and ‘Keounolkong’ × ‘Iksan10’ (K/I). In the K/S population, one QTL was identified for terminal leaflet length (TLL), two for lateral leaflet length (LLL), four for terminal leaflet width (TLW), four for lateral leaflet width (LLW), two for terminal leaflet length/width ratio (TLR) and four for lateral leaflet length/width ratio (LLR), with total phenotypic variations of 7.43, 10.9, 26.57, 23.46, 20.25 and 23.31%, respectively. In the K/I population, two QTLs were identified for TLL, two for LLL, three for TLW, and two for LLW, four for TLR and two for LLR with total phenotypic variations of 29.89, 22.77, 18.5, 12.15, 22.96 and 17.85%, respectively. Only a few QTLs coincided among the leaflet traits and no relationships were observed between the two populations. Many QTLs were associated with leaflet traits but each single QTL made only a minimal contribution. Thus, pyramiding the favourable alleles for leaflet traits in soybean breeding programmes may accelerate vegetative growth and perhaps lead to higher yields by maximizing total photosynthetic performance.
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  • 2
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Artificial hybridization in chickpea (Cicer arietinum L.) is a tedious operation with known low rate of success. A study conducted at the University of Saskatchewan showed that crossing success was influenced by parental identity in this crop. Percent pod set to the total flowers pollinated in chickpea crosses was significantly increased when the smaller-seeded parent was used as the female. Also, success rate increased with the size of the leaf subtending female parent's flowers at the time of crossing.
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  • 3
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The tendency of unpaired meiotic chromosomes to undergo centric misdivision was exploited to translocate leaf rust and stripe rust resistance genes from an Aegilops kotschyi addition chromosome to a group 2 chromosome of wheat. Monosomic and telosomic analyses showed that the translocation occurred to wheat chromosome arm 2DL. The introgressed region did not pair with the corresponding wheat 2DL telosome during meiosis suggesting that a whole arm may have been transferred. Female transmission of the resistance was about 55% whereas male transmission was strongly preferential (96%). The symbols Lr54 and Yr37 are proposed to designate the new resistance genes.
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  • 4
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The presence of gossypol and its derivatives above the WHO/FAO standards (0.02–0.04%) in cotton seed oil and meal limits its usage as food and feed. To the contrary, the presence of pigment glands filled with gossypol and its derivatives helps to protect cotton plants from phytophageous pests. Thus a desirable cultivar would have glandless seeds on a glanded plant. This paper describes results on the successful introgression of this trait from Gossypium bickii into cultivated upland cotton. Five different tri-specific hybrids (ABH1, ABH2, ABH3, ABH4 and ABH5) were obtained by crossing the amphidiploid F1 (G. arboreum × G. bickii) with different gland genotypes of G. hirsutum as male parent. The hybrids were highly sterile, and their chromosome configuration at meiosis metaphase 1 (M1) in pollen mother cell (PMC) was 2n = 52 = 41.04 I + 4.54 II + 0.57 III + 0.04 IV. All five hybrids were similar in morphological characters, except for the gland expression and gossypol contents. The hybrid (ABH3) derived from genotype Gl2Gl2gl3gl3 of upland cotton (a single gene dominant line) had completely introgressed the target trait of G. bickii. While ABH1 and ABH2, which derived from recessive (gl2gl2gl3gl3) or dominant (Gl〈inlineGraphic alt="inline image" href="urn:x-wiley:01799541:PBR1151:PBR_1151_mu1" location="equation/PBR_1151_mu1.gif"/〉Gl〈inlineGraphic alt="inline image" href="urn:x-wiley:01799541:PBR1151:PBR_1151_mu2" location="equation/PBR_1151_mu2.gif"/〉) glandless upland cotton genotypes, had glandless seeds too, but the density and size of the glands on the plant were reduced significantly.
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  • 5
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The objective of this work was to develop homozygous common bean lines carrying angular leaf spot resistance genes derived from the cultivars ‘Mexico 54’, ‘MAR 2’ and ‘BAT 332’ through marker-assisted selection. Molecular markers SCAR OPN02890, RAPD OPE04500 and OPAO12950 linked to the resistance genes of ‘Mexico 54’, ‘MAR 2’ and ‘BAT 332’, respectively, were used in segregating backcross-derived populations to selection. DNA fingerprinting was used to select homozygous BC2F3 and BC1F3 resistant plants genetically closer to the recurrent parent. Two homozygous BC2F2:3 and two and five BC1F2:3 families derived from ‘Ruda’ vs. ‘Mexico 54’ (RM), ‘MAR 2’ (RMA) and ‘BAT 332’ (RB) crosses were selected, respectively. After only one (RMA, RB) or two backcrosses (RM), five and eight BC1F3 lines derived from RMA and RB, respectively, and seven BC2F3 lines derived from RM, with 14.9–16.6, 16.9–18.6 and 9.3–11.1% of relative genetic distances to the recurrent parent were selected. This is the first report of lines resistant to angular leaf spot carrying genes of the cultivars ‘Mexico 54’, ‘MAR 2’ and ‘BAT 332’ developed with the aid of molecular markers.
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  • 6
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four barley doubled haploid populations were produced by anther culture from the reciprocal crosses between two six-row barley cultivars, ‘Plaisant’ and ‘Orria’; the doubled haploid lines (DHLs) derived from each cross were subsequently assigned to weak or vigorous populations according to the weak or vigorous nature of the originating embryos. Well-formed embryos at day 25 on the induction medium were considered vigorous, whereas embryos maturing later were considered weak. The classification of vigorous and weak was closely associated with the ratio of green to albino plantlets regenerated. A random set of 25 DHLs from each of the four populations were selected for field testing in a replicated trial. Furthermore, a second set consisting of a total of 454 unreplicated DHLs from the four populations were also field assessed for grain yield. Distortion during in vitro culture may impede regeneration of a random array of microspores from a given cross, and may bias genetic estimates of specific trait/marker association in genetic studies. However, no significant differences were detected in this study among the four populations for days to heading, height, grain yield and thousand-kernel-weight when measured on the replicated trial of 100 DHLs, nor for grain yield in the second collection of 454 entries. This suggests that the likelihood of producing improved agronomic pure lines is independent of the direction of crossing and, more importantly, independent of the time when embryos matured in the induction media, at least for these particular six-row cultivars and for the anther culture method used.
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  • 7
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 8
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    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To verify the compatibility behaviour of the almond cultivar ‘Francolí’ and to clarify its S genotype a combination of pollination tests, stylar ribonuclease and allele specific PCR analysis was used. ‘Francolí’ was released from IRTA's breeding programme in 1994, having been putatively raised from the cross ‘Cristomorto’ (S1S2) × ‘Gabaix’ (S10S25). This cultivar was also reported to be self-incompatible but revealing only one S band in the zymograms after S-RNases analysis. ‘Francolí’ sets nuts after test crossing with two S1S25 cultivars, having a different genotype from that earlier reported. ‘Francolí’ was also observed to be self-compatible after selfing flowers in the field and in the laboratory. ‘Francolí’ was re-assigned the S1Sf genotype after test crossing, stylar ribonuclease and PCR data analysis. After microsatellite analysis, the self-compatible ‘Tuono’ (S1Sf) cultivar is suggested as the male parent of ‘Francolí’ instead of the earlier reported ‘Gabaix’.
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  • 9
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The genetic control of water-use and photosynthetic traits in Brassica oleracea is resolved by genetic analysis of quantitative trait loci (QTL). Variations in leaf conductance, photosynthetic assimilation rate, leaf thickness and leaf nitrogen content were assessed in a segregating population of F1-derived doubled haploid (DH) B. oleracea lines. In addition, stable carbon isotope ratios in leaf organic material were used as a surrogate measure of plant water-use efficiency. Analysis of an existing linkage map for the population revealed significant QTL on seven linkage groups. Single significant QTL explained between 3.4% and 36.6% of the phenotypic variance in each of the traits measured. The locations of QTL for several traits were found to coincide in a physiologically meaningful way; stable carbon isotope discrimination had QTL co-locating with leaf level water-use efficiency, photosynthetic capacity with leaf thickness and nitrogen content and stomatal density with leaf thickness. Taken together, these results suggest that single genes or clusters of genes at these loci may have an influence on the expression of physiologically related traits controlling water-use and photosynthesis.
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  • 10
    Electronic Resource
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 11
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A method was developed to estimate the relative contribution of increased biomass, harvest index and yield components to seed yield gain of soybean. Data from four field experiments conducted in three soybean production regions in China were used to measure the role of different traits (such as harvest index) in seed yield improvement by applying this method. The results indicated that harvest index was a larger contributor to the progress of soybean yield improvements than biomass in China. However, the estimates of improvements of yield components suggested that there could be several strategies for obtaining high yield. High yielding would be achieved by improving all of the three yield components (pods per plant, seeds per pod and seed weight) simultaneously, or significantly improving one or two of the three components.
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  • 12
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A rapid and efficient microspore culture protocol was applied to produce homozygous progeny of crosses between low erucic canola and high erucic resynthesized rapeseed (Brassica napus L.). Microspores of Canadian cultivars ‘Excel’ and ‘Profit’ as well as three F1 hybrids with the resynthetic line ‘RS239’ were treated with colchicine immediately after isolation. Flow cytometry was applied for early identification of doubled haploid (DH) regenerants. The diploidization rate was subsequently verified by scoring flower morphology. In vitro colchicine treatment had a positive effect on induced diploidization, and was associated with the frequency of preliminary spontaneous diploidization which was, however, determined by the genotype. In addition, the effects of colchicine treatment on embryoid formation and regeneration have been evaluated. The method presented is feasible for commercial large-scale production of DHs in rapeseed as the genotype-specific diploidization can be efficiently balanced by in vitro colchicine treatment. In addition, the use of flow cytometry immediately after in vitro culture allows efficient selection for DHs, thus saving labour and cost and in the laboratory and subsequent greenhouse phase.
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  • 13
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The Acph1 locus of Aegilops tauschii encodes a new electrophoretically ‘fast’ acid phosphatase, whose allelic variation could well be involved in intraspecies differentiation. Genetic mapping via microsatellite (simple sequence repeat) analysis revealed that Acph1 is tightly linked with the marker Xgwm157 near the centromere region of chromosome 2.
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  • 14
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Genomic in situ hybridization (GISH) was used to characterize the chromosome constitutions of individual plants from a set of tetraploid and hexaploid cultivars of Festulolium developed and released in the Czech Republic from hybrids of Lolium multiflorum with Festuca pratensis and F. arundinacea. A simplified GISH protocol readily discriminated parental genomes in the hybrids and facilitated the screening of large numbers of plants per accession. The contribution of parental genomes in the cultivars tested ranged from predominance of chromatin from one of the parents to a more balanced contribution from both parents. However, in none of the cultivars were equal proportions of chromatin from both parents present. The parental contribution to the hybrids was both in the form of complete chromosomes or as chromosome translocations. In hexaploid cultivars from (L. multiflorum × F. arundinacea) × F. arundinacea hybrids the average numbers of complete L. multiflorum chromosomes ranged from 4.95 to 7.5 and the numbers of translocations from 6.33 to 10.21. Two tetraploid cultivars from (L. multiflorum × F. arundinacea) × L. multiflorum hybrids showed a strong prevalence of L. multiflorum chromatin and intergeneric translocations were rare. In the tetraploid cultivar ‘Perun’ of the L. multiflorum × F. pratensis hybrid there were 11.7 chromosomes of L. multiflorum and 14.7 recombined chromosomes on average. Reasons for the domination of one of the parental genomes in hybrid cultivars are not clear and are only partially explained by breeding history. Recombination rates of individual genomes in hybrids involving F. arundinacea were evaluated in double hybridization experiments. The results indicated a strong affinity of the L. multiflorum genome for the F. pratensis genome present in F. arundinacea and little affinity for the F. glaucescens genome. This suggests that introgressions from F. arundinacea into L. multiflorum are primarily limited to the F. pratensis genome which can be more readily accessed in L. multiflorum × F. pratensis hybrids.
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  • 15
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Nicotiana glauca, a wild relative of Nicotiana tabacum, is an attractive potential source of black root rot (Thielaviopsis basicola)-resistant germplasm. Moreover, it shows a resistance or tolerance to PVY, TEV, anthracnose and powdery mildew. In this study its potential as a source of resistance to black root rot caused by Th. basicola was investigated. Nicotiana glauca GG (2n = 2x = 24) was crossed as male with two N. tabacum TT (2n = 4x = 48) flue-cured cultivars: ‘BY103’ and ‘K 326’, both of which are susceptible to black root rot. Amphihaploid F1 TG (2n = 3x = 36), amphidiploid TTGG (2n = 6x = 72) and sesquidiploid TTG (2n = 5x = 60) hybrids were obtained. The resultant sesquidiploid hybrids were used as maternal components in backcrossing to N. tabacum and a segregating post-sesquidiploid TTg (2n = 5x = 52–54) offspring was obtained. Amphihaploids exhibited a level of resistance to black root rot characteristic of N. glauca. The expression of resistance varied in the sesquidiploid generation, possibly reflecting cytological instabilities in that progeny. A wide variation in response to black root rot was found for post-sesquidiploids a clear reflection of extensive chromosome segregation in that hybrid generation.
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  • 16
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The genetic diversity of 66 Phaseolus genotypes was investigated, which included 14 local varieties of Phaseolus vulgaris and nine local varieties of P. coccineus, collected in Marche, central Italy. Their genetic diversity was assessed using three types of molecular marker: inter simple sequence repeats (ISSRs), nuclear gene-tagged simple sequence repeats (SSRs) and chloroplast simple sequence repeats (CpSSRs). Phaseolus vulgaris shows a higher genetic diversity than P. coccineus for the SSRs and CpSSRs, but not for the putative neutral ISSR markers. These data suggest that selection by farmers and adaptation to heterogeneous environments has maintained the diversity in landraces of the common bean. Comparing genetic diversity in Marche with that of the American controls reveals that 71% of the local P. vulgaris varieties in Marche are of Andean origin. The two gene pools of the common bean can be found on the same farm, and there is some evidence of past hybridization events between these two gene pools.
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  • 17
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The gene Lr34 has contributed to durable resistance to leaf rust caused by Puccinia triticina in wheat worldwide. The closely associated leaf tip necrosis is generally used as the gene's marker. Lr34 has been postulated in many Indian bread wheat cultivars including ‘C 306’, based on the associated leaf tip necrosis and a few other field and glasshouse observations. The present study showed monogenic control of adult-plant resistance in ‘C 306’ to leaf rust pathotype 77-5 (121R63-1). The F2 segregation in the crosses between ‘C 306’ and the two known carriers of Lr34, ‘Line 897’ and ‘Jupateco 73’‘R’ fitted a digenic ratio. The F3 families derived from the susceptible F2 segregants were true breeding for susceptibility, proving the absence of Lr34 in ‘C 306’. The cross between ‘Line 897’ and ‘Jupateco 73’‘R’ did not segregate for susceptibility. Resistance in the cross ‘Agra Local’ (susceptible) × ‘C 306’ was associated with leaf tip necrosis, showing that the leaf rust resistance gene in ‘C 306’ was associated with leaf tip necrosis, but was different from Lr34. This gene is being temporarily designated as Lr‘C 306’. Hence, leaf tip necrosis cannot be considered as an exclusive marker for selecting Lr34 in wheat improvement.
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  • 18
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: ‘Ketan Nangka’, the donor of the wide compatibility gene (WCG) showed typical hybrid sterility when crossed to a landrace, ‘Bai Mi Fen’, of Yunnan province in China. A genome-wide analysis was performed for a backcrossed population of ‘Ketan Nangka’/‘Bai Mi Fen’//‘Ketan Nangka’ using a total of 143 simple sequence repeat markers and an expressed sequence tagged marker to cover the entire rice linkage map. As a result, two independent loci were found to cause hybrid sterility via female gamete abortion. The locus on chromosome 4 may correspond to S9, but the other, on chromosome 2, was different from all the previously reported hybrid sterility loci and was designated as S29(t) following the hybrid sterility nomenclature. On the basis of allelic interaction which causes female gamete abortion, two alleles were found: S29kn(t) in ‘Ketan Nangka’ and S29bi(t) in ‘Bai Mi Fen’. In the heterozygote, S29kn(t)/S29bi(t), which was semi-sterile, female gametes carrying S29bi(t) were aborted. An Aus variety from the Indian subcontinent, ‘Dular’, was found to have a neutral allele, S29n(t). Two molecular markers, RM185 and RM425, linked to S9 and S29(t), respectively, will be useful for marker-aided transfer of WCGs in hybrid rice breeding.
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  • 19
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To identify markers for the everbearing gene in strawberries, 199 F1 progeny plants were produced from a cross between ‘Ever Berry’ (a Japanese everbearing strawberry) and ‘Toyonoka’ (a Japanese Junebearing strawberry) as the experimental population. The results of flowering tests produced 97 everbears and 102 Junebears. The chi-square test gave a goodness of fit for the expected ratio of 1 : 1 for everbears to Junebears, suggesting the inheritance of the everbearing trait is controlled by a monogenic dominant gene. RAPD analyses on this trait were carried out using ‘Ever Berry’ and ‘Toyonoka’. Seventy-one primers, which produced 89 polymorphic fragments between the two parents, were identified from a total of 175 primers. Five markers relating to the everbearing trait were selected from 26 of the 199 progeny plants. The remaining 173 seedlings were analysed with these five markers and a linkage map was constructed using all of the 199 F1 progeny plants. The length of this linkage group is 39.7 cM. The closest markers found, OPE07-1 and OPB05-1, are respectively mapped at 11.8 and 15.8 cM on each side of the everbearing gene.
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  • 20
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Significant progress has been made in understanding the structure of high molecular weight (HMW) glutenin subunits and their role in determining the end use quality of wheat grains. However, few reports have dealt with the development and characterization of knock out mutants for HMW glutenin subunit genes. Here, the molecular analysis of MB14, a mutant derived from an elite Chinese wheat variety Xiaoyan 54 through chemical mutagenesis is described. SDS-PAGE and Western blot experiments revealed that, in the seeds of homozygous MB14 plants, the expression of the 1Bx14 subunit was specifically blocked whereas the remaining four subunits (1Ax1, 1By15, 1Dx2, 1Dy12) accumulated to levels comparable to those in the wild type plants. The 5′-flanking region and the open reading frame (ORF) of the mutant 1Bx14 allele were amplified and compared to the corresponding regions of wild type 1Bx14. The nucleotide sequences of the 5′-flanking regions from the mutant and wild type 1Bx14 alleles were identical. However, the ORF of the mutant allele differed from that of the wild type 1Bx14 by three point substitutions, one of which resulted in a premature stop codon in the mutant ORF. Interestingly, the mutant 1Bx14 allele was still transcribed in the developing seeds, but no truncated translation product could be detected by Western blot analysis. Potential application of the 1Bx14 knock out mutant in studying the biological function of 1Bx14 and its contribution to the end use quality control in hexaploid wheat is discussed.
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  • 21
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    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Safflower (Carthamus tinctorius L.) seeds have a tocopherol fraction dominated by α-tocopherol, which accounts for more than 95% of the tocopherols. α-Tocopherol exerts a high vitamin E activity, but a low in vitro antioxidant action. For non-food applications, replacement of high α-tocopherol by γ-tocopherol is preferred. Because of the limited variability found in germplasm of cultivated safflower, the objective of the present research was to search for variability for tocopherol profile in germplasm of wild safflower species. Bulk seed samples of 77 accessions from six species were analysed for total tocopherol content and profile. One accession of C. oxyacanthus showed increased γ-tocopherol content (36%). Single-seed analysis showed the existence of a bimodal distribution, which included high α-tocopherol (〉90%) and high γ-tocopherol (HGT) seeds (〉85%). Plants from the HGT seeds bred true for the trait and showed introgression of cultivated safflower, which allowed a rapid selection for cultivated safflower traits. Genetic analysis revealed that the HGT trait was controlled by partially recessive alleles at a single locus Tph1, which will facilitate the transference of the novel trait to diverse safflower germplasms.
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  • 22
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Male fertility restoration in new types of sorghum cytoplasmic male sterility-inducing cytoplasms (A4, ‘9E’, ‘M35’), characterized by the formation of non-dehiscent anthers, is difficult. Lines with fertility-restorer genes for these unique cytoplasms do occur, but rarely, and when found tend to be unstable in their inheritance and expression. The aim of this research was to explore reasons for this instability. Seven lines in three unique cytoplasms, ‘9E’, A4 and ‘M35’, and six lines that restore with these cytoplasms were grown at the Agricultural Research Institute for South-East Region in Saratov, Russia from 1993 to 2004. Levels of male fertility restoration and various environmental factors were recorded. It is reported that for sorghum hybrids in the A4, ‘9E’ and ‘M35’ male-sterile cytoplasms, the level of plant male fertility is determined by the level of water available to plants during anther and pollen formation that which ‘switches on’ the expression of fertility-restoring genes, and is possibly involved in an unusual type of male fertility inheritance in these cytoplasms. The creation of reliable line-fertility restorers capable of the restoration of male fertility of F1 hybrids in ‘M35’ cytoplasm under conditions of water stress is also reported. Current research explore mechanisms involved possible in responses to water levels at various growth stages and their influence on fertility within these cytoplasms.
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  • 23
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In Brassica rapa L., intraspecific variation in the ultraviolet (UV) colour proportion invisible to humans but visible to insect pollinators, was investigated by UV-photographing. After digitization of each photograph, the UV-absorbing area (UVA) and total flower area (FA) were evaluated by means of image analysis. The ratio of UVA to FA represented the UV colour proportion (UVP). Nested anova indicated that the proportion of the variance caused by the genotypic effect was largest for UVP, and anova indicated that the F-value for UVP was larger than that for FA. These results indicate that UVP is very stable within a genotype. That is, there was almost no additional variation caused by plants and branches. In contrast, UVP varies widely between genotypes with respect to FA. The large differences in the UVP of B. rapa flowers are likely to be distinguishable by insect pollinators, which might use these differences as visual cues in their foraging flight.
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  • 24
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Hessian fly [Mayetiola destructor (Say)] is one of the major insect pests of wheat (Triticum aestivum L.) worldwide. Hessian fly resistance gene H9 was previously reported to condition resistance to Hessian fly biotype L that is prevalent in many wheat-growing areas of eastern USA and an RAPD marker, OPO051000, linked to H9 in wheat was developed using wheat near-isogenic lines (NILs). However, marker-assisted selection (MAS) with RAPD markers is not always feasible. One of the objectives in this study was to convert an RAPD marker linked to the gene H9 into a sequence characterized amplified region (SCAR) marker to facilitate MAS and to map H9 in the wheat genome. The RAPD fragment from OPO051000 was cloned, sequenced, and converted into a SCAR marker SOPO05909, whose linkage relationship with H9 was subsequently confirmed in two F2 populations segregating for H9. Linkage analysis identified one sequence tagged site (STS) marker, STS-Pm3, and the eight microsatellite markers Xbarc263, Xcfa2153, Xpsp2999, Xgwm136, Xgdm33, Xcnl76, Xcnl117 and Xwmc24 near the H9 locus on the distal region of the short arm of chromosome 1A, contrary to the previously reported location of H9 on chromosome 5A. Locus Xbarc263 was 1.2 cM distal to H9, which itself was 1.7 cM proximal to loci Xcfa2153, Xpsp2999 and Xgwm136. The loci Xgwm136, Xcfa2153 and SOPO05909 were shown to be specific to H9 and not diagnostic to several other Hessian fly resistance genes, and therefore should be useful for pyramiding H9 with other Hessian fly resistance genes in a single genotype.
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  • 25
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: This study was undertaken to estimate the level of variation among and within 33 local populations belonging to seven Italian common bean landraces, by analysing the polymorphism of seed storage proteins, simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) molecular markers. The nutritional seed quality of the landraces studied was also investigated. Results showed that the landraces retain a considerable level of heterogeneity. Use of both biochemical and molecular approaches provided a picture of the genetic diversity of each landrace. Cluster analyses based on Nei's genetic distances and Jaccard's similarity index as defined by SSR and AFLP markers, respectively, showed that all populations clustered into two groups corresponding to the Andean and the Mesoamerican gene pools. Knowledge of the genetic structure of a landrace is fundamental in elaborating strategies, which involve the local farmers, allowing us to improve and, at the same time, safeguard the genetic integrity of landrace genetic resources.
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  • 26
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Diallel crosses (without reciprocals) were made among 10 different barley genotypes with genetic variability for spot blotch resistance. Forty-five F1 hybrids and their parents were assessed for their combining abilities for the disease resistance. Three experiments, two in a growth chamber on detached leaf and seedlings tests and one in the field on adult plant stages, were undertaken using a randomized complete block design with five replicates. A mixed conidial suspension of nine virulent isolates of the pathogen was used for inoculation. Statistical analysis showed genetic variability for spot blotch resistance. Results showed that the cultivar Banteng, the Ethiopian line CI-5791 and the Syrian line 79-SIO-9 had partial resistance in all experiments. General combining ability was significant, with either positive or negative values. Resistant genotypes show favourable GCA-effects, and they could therefore be successfully used for breeding purposes.
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  • 27
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isolation and characterization of microsatellites was analysed in Bambusa arundinacea and cross species amplification studied in other bamboos. Microsatellites, tandem repeats of short nucleotide (1–6 bp) sequences, are the DNA marker of choice because of their highly polymorphic, ubiquitous distribution within the genome, ease of genotyping through Polymerase chain reaction, selectively neutral, co-dominant and multiallelic nature. Six microsatellites, three polymorphic and three monomorphic have been characterized for the first time in a bamboo species, Bambusa arundinacea belonging to the family Poaceae. The numbers of alleles per locus ranged from 2 to 13. Cross species amplification was tested in 18 other bamboo species. Monomorphic simple sequence repeats (SSRs) were found to be cross amplified in most of the species tested and polymorphic ones in only three to four species. The utility of SSR loci in a genetic diversity study of B. arundinacea and other cross-amplified bamboo species has been discussed. This study will help in population genetic studies in bamboo species.
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  • 28
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Crown rot, caused by Fusarium pseudograminearum, is an important disease of wheat in Australia and elsewhere. In order to identify molecular markers associated with partial seedling resistance to this disease, bulked segregant analysis and quantitative trait loci (QTL) mapping approaches were undertaken using a population of 145 doubled haploid lines constructed from ‘2-49’ (partially resistant) × ‘Janz’ (susceptible) parents. Phenotypic data indicated that the trait is quantitatively inherited. The largest QTLs were located on chromosomes 1D and 1A, and explained 21% and 9% of the phenotypic variance, respectively. Using the best markers associated with five QTLs identified by composite interval mapping, the combined effect of the QTLs explained 40.6% of the phenotypic variance. All resistance alleles were inherited from ‘2-49’ with the exception of a QTL on 2B, which was inherited from ‘Janz’. A minor QTL on 4B was loosely linked (19.8 cM) to the Rht1 locus in repulsion. None of the QTLs identified in this study were located in the same region as resistance QTLs identified in other populations segregating for Fusarium head blight, caused by Fusarium graminearum.
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  • 29
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The Russian wheat aphid (RWA), is a serious threat to wheat production worldwide. The identification of a new RWA biotype in the USA virulent to all commercially grown winter wheats poses new challenges to wheat breeders. Wheat germplasm was evaluated to identify accessions resistant to the new virulent RWA isolate (biotype 2). Eleven biotype 1-resistant wheats and one susceptible check were challenged with RWA biotype 2. Two resistant wheat entries were identified (one highly resistant and one moderately resistant). This information is useful to wheat breeders searching for sources of resistance to the new RWA biotype to incorporate into their breeding programmes.
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  • 30
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Stylosanthes guianensis, belonging to the genus Stylosanthes, is one of the most important tropical forage legumes and is native to South and Central America and Africa. Anthracnose, caused by the fungus Colletotrichum gloeosporioides (Penz.) Sacc., is a major constraint to the extensive use of Stylosanthes as tropical forage. Forty-two accessions of S. guianensis were assessed with amplified fragment length polymorphism (AFLP) for genetic diversity and for resistance to anthracnose. In AFLP analysis, four selective primer combinations screened from 96 primer combinations were used to analyse these accessions, and a total of 225 clear bands were used for genetic similarity (GS) analysis, showing a 95.5% level of polymorphism on average. GS from 31.0% to 95.0% among the accessions was calculated with ntsys-pc software. The dendrogram was constructed with unweighted pair group method of averages (UPGMA) based on the AFLP data, and five clusters were defined at 48% GS. Two typical strains of C. gloeosporioides from Stylosanthes in China were used for anthracnose resistance screening. Most of the plant accessions showed variation in the reaction to two strains, and the correlation of resistance had a value of 0.904 (P 〈 0.01), suggesting common resistance to the two strains. The resistance accessions were randomly distributed in different groups of UPGMA clustering. These results demonstrate that AFLP analysis is an efficient method for evaluating the genetic diversity among S. guianensis accessions.
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  • 31
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: General knowledge of the closed flowering trait, or cleistogamy, of barley is still limited. The relationship between cleistogamy and spike morphology characters was studied and linkage of cleistogamy genes with a highly significant quantitative trait locus (QTL) for rachis internode length on the long arm of chromosome 2H was detected. The mapping populations consisted of 129 doubled haploid lines of ‘Mikamo Golden’ × ‘Harrington’ and 150 F2 plants of ‘Misato Golden’ × ‘Satsuki Nijo’. The phenotypic variance explained by this QTL accounted for 77.5% and 82.6% of the variance in rachis internode lengt, respectively, in these two populations. The peaks of the QTL coincided with the positions of the cleistogamy gene loci.
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Among many species in the genus Tobamovirus, Kyuri green mottle mosaic virus (KGMMV) is known as a major seed-borne virus infecting cucurbit plants, including melon, which possesses no known resistance. The identification of genetic resistance to KGMMV in Indonesia (KGMMV-YM) in melons is of economic importance. Thus, 45 melon cultigens collected from 17 Asian countries were screened by manual virus inoculation and resistance to KGMMV-YM was examined by visual observation, enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction (RT-PCR). The results show that resistance to KGMMV-YM was found in ‘Mawatauri’, ‘Kohimeuri’, PI 161375 and PI 371795, and analysis by RT-PCR showed the presence of KGMMV-YM in susceptible cultigens as early as 3 days after leaf inoculation.
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  • 34
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two genetic linkage maps of Zea mays were constructed: one population comprised 94 F2 individuals of a dent ‘B64’ × teosinte (Z. mays ssp. huehuetenangensis) cross while the second consisted of 94 F2 individuals of a ‘B64’ × Caribbean flint ‘Na4’ cross. The level of polymorphism was higher in the ‘B64’ × teosinte combination than the ‘B64’ × ‘Na4’ combination. In the ‘B64’ × teosinte cross, a total of 338 amplified fragment length polymorphism (AFLP) and 75 simple sequence repeat (SSR) markers were mapped to 10 chromosomes, which covered 1402.4 cM. In the ‘B64’ × ‘Na4’ cross, a total of 340 AFLP and 97 SSR markers were mapped to 10 chromosomes, covering 1662.8 cM. Segregation distortion regions were found on chromosomes 4, 5 and 8 in the ‘B64’ × teosinte cross and on chromosome 9 in the ‘B64’ × ‘Na4’ cross. Comparison of the two maps revealed that the maize × teosinte map was 11.5% shorter than the maize × maize map. The maps generated in this study may be useful to identify genes controlling flooding tolerance.
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  • 35
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Camelina is an alternative oilseed crop species with limited information about the origin and diversity of available germplasm. Therefore, a set of 130 camelina accessions from a world collection was evaluated for oil content, protein content and 1000-seed weight in field experiments grown in three macro-environments in Austria. Based on phenotypic data, accessions were categorized into four groups with different seed characteristics using k-means cluster analysis or principal component extraction. Subsequently, a representative set of 41 accessions was subjected to random amplified polymorphic DNA (RAPD) analysis. Of 24 primers, 15 were polymorphic producing a total of 30 marker loci. Genetic distance estimates between the 41 accessions were calculated, based both on RAPD polymorphism and on seed quality characteristics, and dendrograms were generated for comparison. Similarities were found between the two different clustering approaches, and grouping was partly in agreement with pedigree information or geographic origin. However, as the two estimates of diversity sampled different segments of the genome, i.e. regions coding for seed characteristics or phenotypically neutral genomic regions highlighted by discrete markers, the correlation between the two distance matrices was low.
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  • 36
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Recessively inherited gene Sr2 has provided the basis of durable resistance to stem rust (caused by Puccinia graminis tritici) in wheat (Triticum aestivum L.) worldwide. The associated earhead and stem melanism or ‘pseudo-black chaff’ is generally used as a marker for this gene. Sr2 has been postulated in many wheat cultivars of India including ‘Lok 1’, based on associated pseudo-black chaff in adult plants, and leaf chlorosis in seedlings. However, dominant inheritance of the resistance factor operating in ‘Lok 1’, and a 13 : 3 (resistant : susceptible) F2 segregation in the ‘Sr2-line’ (‘Chinese Spring’6 × ‘Hope’ 3B) × ‘Lok 1’ cross confirmed that Sr2 was absent in ‘Lok 1’. Susceptible plants with a pseudo-black chaff phenotype were observed in F2 populations of ‘Agra Local’ (susceptible) × ‘Lok 1’, and the ‘Sr2-line’ × ‘Lok 1’ crosses. Most of the F3 families derived from the susceptible F2 segregants with pseudo-black chaff phenotypes were true breeding for the expression of pseudo-black chaff with susceptibility to stem rust. Thus, linkage of pseudo-black chaff with Sr2 in wheat can be broken, and hence, caution may be exercised in using pseudo-black chaff as a marker for selecting Sr2 in breeding programmes.
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  • 37
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The bread wheat cultivar ‘Thatcher’ is documented to carry the gene Lr22b for adult-plant resistance to leaf rust. Seedling-resistance to leaf rust caused by Puccinia triticina in the bread wheat cultivar ‘Thatcher’, the background parent of the near-isogenic lines for leaf rust resistance genes in wheat, is rare and no published information could be found on its genetic basis. The F2 and F3 analysis of the cross ‘Agra Local’ (susceptible) × ‘Thatcher’ showed that an apparently incompletely dominant gene conditioned seedling-resistance in ‘Thatcher’ to the three ‘Thatcher’-avirulent Indian leaf rust pathotypes – 0R8, 0R8-1 and 0R9. Test of allelism revealed that this gene (temporarily designated LrKr1) was derived from ‘Kanred’, one of the parents of ‘Thatcher’. Absence of any susceptible F2 segregants in a ‘Thatcher’ × ‘Marquis’ cross confirmed that an additional gene (temporarily designated LrMq1) derived from ‘Marquis’, another parent of ‘Thatcher’, was effective against pathotype 0R9 alone. These two genes as well as a second gene in ‘Kanred’ (temporarily designated LrKr2), which was effective against all the three pathotypes, but has not been inherited by ‘Thatcher’, seem to be novel, undocumented leaf rust resistance genes.
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  • 38
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Interspecific hybridization is known to improve productivity and resistance to diseases in many crops. Therefore, an attempt was made to introgress productivity and disease resistance into chickpea from wild Cicer species. The true F1 hybrids of cultivated chickpea genotypes ‘L550’ and ‘FGK45’ with C. reticulatum were backcrossed twice to their cultivated female parents to minimize the linkage drag of undesirable wild traits. The pedigree method was followed to advance the segregating populations from straight crosses (without backcross) and BC1/BC2 generations to F5–F7. The interspecific derivatives recorded up to a 16.9% increase over the check cultivars and a 25.2% increase over the female parent in a preliminary yield evaluation trial. Of the 22 interspecific derivatives thus derived, four desi and two kabuli lines were further evaluated for seed yield in replicated trials at three diverse locations. These lines possess a high degree of resistance to wilt, foot rot and root rot diseases, and recorded a 6.1–17.0% seed yield increase over the best check cultivars.
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  • 39
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sixteen sweet potato varieties were evaluated for fresh storage root yield in 20 trials during 2000–2001 for three seasons in four locations in Uganda. Of the 16 varieties, 11 were developed by farmers and five by a central breeding programme. The behaviour of the varieties was quantified in terms of wide adaptation (genotypic mean across trials), specific adaptation (genotypic predictions for specific locations) and stability (Shukla stability variance). With respect to all three aspects of yield behaviour, farmer varieties performed on average better than the official varieties. The results illustrate the potential that farmer varieties can have in the improvement of sweet potato in Uganda and other regions where high diversity of sweet potato landraces exists.
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  • 40
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    Plant breeding 124 (2005), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A compressed lamina mutant at a locus named COLA was selected in an inbred population of the cultivated carrot Daucus carota sativus Hoffm. Whereas segregation analyses of F2 progenies, as well as corresponding F3 families, indicated a monogenic recessive inheritance, detailed morphological and histological analyses revealed pleiotropic effects of the COLA gene. The mutant plants exhibited a semi-dwarf phenotype, shortened epidermal and leaf parenchyma cells and significantly reduced lamina dimensions. Furthermore, the mutant developed stipules and hypogynous flowers, contrary to the stipule-less leaves and epigynous flowers of the wild type. Using bulked segregant analyses, thirty-nine marker candidates were detected using 180 RAPD primer and 56 AFLP primer pairs. Twelve primers were linked to the COLA locus and mapped in a linkage group with a total length of 44.2 cM. Potential application of the compressed lamina mutant in carrot research is discussed.
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  • 41
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three near-isogenic lines (NILs) of wheat involving Glu-B1 and Glu-D1 alleles were used to study the genetic contribution of high molecular weight glutenin subunits (HMW-GS) to gluten strength. The HMW-GS composition of each NILs was determined by SDS-PAGE. No significant differences were found in grain protein contents among the NILs. Gluten strength and dough-mixing properties were measured by the Farinograph, the Extensograph, and SDS-sedimentation (SDS-SE). Results indicated that line 2, containing the Glu-1B 14 + 15 and Glu-1D 5 + 10 combination of subunits, had higher values for flour quality, dough rheological parameters, and bread-baking quality when compared with lines 8 and 13. Line 8, containing Glu-1B 7 + 9 and Glu-1D 5 + 10, was better than line 13 with the Glu-1B 14 + 15 and Glu-1D 10 combination. Some major parameters appeared significantly different. The presence of Glu-1B 14 + 15 was associated with higher dough strength based on SDS-SE volume and several rheological parameters when compared with Glu-1B 7 + 9. Lines with subunit 10 at Glu-D1 performed significantly worse than those with 5 + 10 in gluten index, SDS-SE volume, Farinograph stability time, Extensograph area and bread-baking quality.
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  • 42
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The monthly variation in reproductive cycle, condition factor (fatness), gonad index (GI), ovarian egg diameter and biochemical composition [RNA, DNA content and their ratio RNA/DNA (R/D)] were compared to determine the spawning season of the purplish Washington clam, Saxidomus purpuratus, collected from the west sea of Taean, Korea. All these factors were correlated to the spawning season from May to October. Monthly variation and concentration of the R/D ratio especially represented indicative information about the processes of gonad development and spawning patterns. The increases in R/D ratio in the ovaries corresponded with increases in ovarian egg diameters and the GI during the spawning season. The peak of R/D and RNA content in females was a good indicator of sexual maturation and the DNA content in males. During winter between November and January, the value of fatness, GI, egg size, RNA and DNA content were lower than in other seasons, indicating depletion of energy reserves and lower metabolic demands because of colder temperatures.
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  • 43
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Several USA state, federal, and Canadian agencies study lake sturgeon (Acipenser fulvescens) within the St Clair River and Lake St Clair, collectively referred to hereafter as the St Clair River (SCR) system. Previously, there has been no set standard for determining condition for SCR system lake sturgeon. Condition measures the variation from the expected weight for length as an indicator of fatness, general well-being, gonad development, etc. The aim of this project was to determine the length–weight relationship of lake sturgeon caught from the SCR system, from which a relative condition factor (Kn) equation could be derived. Total length (TL, mm) and weight (W, kg) were measured for 1074 lake sturgeon (101 males and 16 females were identifiable) collected by setline and bottom trawl from the SCR system in May–September, 1997–2002. Analysis of covariance found no difference in the length–weight relationship between sampling gear or sex. Least-squares regression of log10W × log10TL produced the overall equation logW = 3.365logTL − 9.320. Using the exponential form of the slope and y-intercept, relative condition factor for lake sturgeon from the SCR system can be calculated as Kn = W/[(4.786 × 10−10)(TL3.365)]. Equations for males and females were also developed. Overall, body condition was significantly correlated with both age and girth; no significant difference in Kn by sex was found. In general, the SCR lake sturgeon population was near the upper ends of growth and condition ranges listed in the literature, comparable with those populations that are at similar latitudes. Although condition factors should be interpreted with caution, proper use of a standard equation provides a non-lethal measure of overall fish health that can be used by biologists and managers in ongoing efforts to restore lake sturgeon throughout the Great Lakes.
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  • 44
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Samples of Paralonchurus brasiliensis Steindachner, 1875 (627 females; no males) were taken monthly from June 1997 to May 1998 at the fishing pier of Cidreira – Rio Grande do Sul (30°08′S; 50°11′W) in southern Brazil. The fish were captured by sport fishermen using different sizes of hook-and-line. The reproductive period of P. brasiliensis extends from August to March, presenting greater activity from October to February. Females reach first maturation at 15.4 cm total length (TL). Of the 466 scales analyzed, one to six annuli were found. Growth annuli are formed between June and August, corresponding to the winter period (southern hemisphere), with the first visible annulus being formed when the fish are 1.5 years old. The length growth curve for P. brasiliensis females is described as: Lt = 20.0 (1 − e−0.564×t), TL in centimeters and age in years. The undersize asymptotic length may be a result of excessive fishing mortality on the studied population.
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  • 45
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Offshore production offers a new perspective for mussel aquaculture in the German Bight (North Sea) as no expansion is possible in the intertidal and subtidal zone of the Wadden Sea because of restrictions on the number of licenses. The development of offshore wind farms offers a unique opportunity because of the associated infrastructure. Service platforms, as well as the pylons themselves, offer perfect structures for mooring longlines and other culture units. One of the advantages of offshore culture may be a lower parasite load in offshore mussels compared with mussels produced under traditional inshore bottom culture. By sampling mussel spat from offshore suspended buoys or collectors, we simulated an offshore culture situation and compared parasite infestation rates with those in mussel spat obtained from suspended inshore buoys or collectors, in mussels from inshore benthic subtidal beds and from inshore benthic intertidal mussel beds. Mussels from offshore sites were free of trematodes and shell-boring polychaetes. Parasitic copepods only occurred at a single offshore site, on a 20-year-old research platform, but not on buoys or collectors exposed for shorter time periods. All three monitored parasite taxa were present at all other sites. The highest prevalence was found for trematodes in inshore benthic intertidal mussels (78.7 ± 6.4%) and locally reached 100%. Through a variety of detrimental effects, trematodes, parasitic copepods and shell-boring polychaetes are known to affect growth performance and product quality. We therefore propose that offshore mussel production could be a promising culture procedure because it seems to result in lower parasite burden than at traditional culture sites. Whether offshore production also results in better survival and growth, compared with inshore mussel culture on a commercial scale, needs to be investigated further.
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  • 46
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The distribution and abundance patterns as well as the feeding ecology of two flatfishes, Platichthys flesus (Linnaeus, 1758) and Solea solea (Linnaeus, 1758), were studied in the Douro estuary and adjacent coastal waters (Portugal) in order to evaluate the niche overlap between these species. Fish were collected during sampling surveys conducted every 2 months between November 2000 and March 2002 using a 12-m otter-trawl with 18 mm stretched mesh size, at 12 sites within the estuary and six sites in the adjacent coastal area. The highest densities of P. flesus were registered in the middle estuary; a peak density of 9.12 ind 1000 m−2 was obtained in October 2001. Solea solea densities were low compared with P. flesus; the highest values were also recorded in the middle estuary in October 2001. For both species, the proportion of juveniles was above 90% within the estuary. Highest densities of P. flesus and S. solea were recorded at sites with low water depth and high densities of crustaceans and polychaetes, the main food items in the diet of these species. High values of spatial and diet niche overlap were found between these two flatfishes (0.97 and 0.92, respectively).
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  • 47
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The diet of seven fish species from the Río de la Plata was studied to evaluate their behaviour as predators on the invasive bivalves Corbicula fluminea and Limnoperna fortunei. It was concluded that the teleosts Pterodoras granulosus, Pimelodus maculatus, Paraloricaria vetula and Ricola macrops prey upon C. fluminea, whilst Leporinus obtusidens, Rhinodoras dorbignyi and Brochiloricaria chauliodon feed on Lim. fortunei. These fish species had altered their diets to consume large amounts of these molluscs. Three of the fishes recorded here were previously unknown to feed on molluscs. The fishes studied here prey upon molluscs that ingest trace metals with their food items, thus monitoring the situation is suggested as several of these fish species are caught for human consumption.
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  • 48
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The growth performance of juvenile sturgeon (Acipenser gueldenstaedtii Brandt & Ratzenburg, 1833) was studied in experimental net cages (2 m × 2 m × 2 m) in freshwater Sapanca Lake. Fertilized eggs were imported from Krasnodar, Russia in January 2001. Two experimental stocking densities were employed (12 and 8 ind. m−3). Sturgeon were hand-fed a pelleted commercial trout feed twice daily throughout the 203-day experiment period. Fish were grown at ambient temperature (12.7–28.5°C) and natural photoperiod. Initial and final mean body weights were 279.5 g (±31.27) and 1112.8 g (±234.77) in the first group, and 271.1 g (±28.21) and 1140.5 g (±213.31) in the second group, respectively. Results were a fourfold biomass density increase in both trials from an initial 3.48 to 12.4 kg m−3 (high density) and 2.2 to 8.8 kg m−3 (low density). Mean specific growth rate (SGR) was determined as 2.69% day−1 in both groups. Mean condition factor (CF) at the end of the experiment was 0.36 ± 0.05 in the high-density group and 0.41 ± 0.05 in the low-density group. Mean feed conversion ratio (FCR) was calculated to be 5.7 and 5.8, respectively. Survival was 92.7% in the first group (high density) and 96.1% in the second group (low density). The results demonstrated that sturgeon juveniles can be raised in cages and that growth performance (CF, SGR and FCR) and mortality of sturgeon were not significantly different at the two stocking densities (P 〉 0.05) tested.
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  • 49
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: This study reports weight–length relationships (WLR) for Salmo trutta (Linnaeus, 1758), Phoxinus phoxinus (Linnaeus, 1758), Chondrostoma Chondrostoma miegii (Steindachner, 1866), Barbus graellsii (Steindachner, 1866), Barbatula barbatula (Linnaeus, 1758) and Gobio gobio (Linnaeus, 1758). Specimens were collected by electrofishing during summer between 1996 and 1998. The b values in the WLR W = aLb varied between 2.97 and 3.42. To the best knowledge of the authors, this study presents the first reference on WLRs for four of the species in Spanish waters.
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Total length (TL) at first sexual maturity was estimated for Atlantoraja castelnaui and Dipturus chilensis from the south-western Atlantic Ocean. Total length at which 50% of the females were captured (50% c) was less than the TL at first sexual maturity (ML) in both species. Immature females are being captured and thus will not become sexually mature to reproduce because of early fishing mortality. Females of A. castelnaui and D. chilensis reach sexual maturity at TLs of 110–114 cm and 102–106 cm, respectively. Males mature with TL between 91 and 95 cm for A. castelnaui and between 83 and 87 cm for D. chilensis.
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  • 51
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A laboratory experiment was carried out to quantify and compare the physical damage (measured as scale loss), recovery and survival of two size categories [small: 48–85 mm total length (TL); large: 78–148 mm TL] of 0-group mulloway, Argyrosomus japonicus, after simulated escape through square-shaped mesh (bar length of 21.5 mm). Regardless of their size, fish that were fatigued to exhaustion and forced through square meshes sustained significantly more (i.e. 〉1.8 times) scale loss than did control fish that were only fatigued. However, the total scale loss incurred was 〈5% and significantly improved 7 days after treatment. Survival rates over a 2-week observation period were 100 and 〉97% for treatment and control fish, respectively. The results support the utility of square mesh for reducing the prawn-trawl fishing mortality of unwanted bycatch.
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  • 52
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Investigations on the reproduction biology of cod in the Baltic Sea (Gadus morhua L. and G. morhua callarias L.) were carried out beginning in 1993, with yearly sampling of gonads to estimate the potential absolute and relative individual fecundity in the Belt Sea (subdivision, SD: 22) and in the Arkona Sea (SD: 24). Additional samples were taken in the Bornholm Sea (SD: 25). The potential absolute fecundity of the total area of investigation is described by the regression models: Fa = 6.89 × TL3.09 and Fa = −40 586 + 977.7 × W. As a further result the mean relative fecundity was 978 oocytes per gram body weight. Furthermore, it was shown that there was a difference in development of the potential individual fecundity between the Belt Sea cod and the original eastern Baltic cod stock. In contrast to the stable fecundity of cod in the Belt Sea, the fecundity of the large females in the Bornholm Sea significantly increased from 1993 to 1999. This change is related to a decrease in the dry weight of oocytes in the ovaries. In the same period this cod stock dramatically decreased.
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  • 53
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Growth, productivity and potential for exploitation of the clam Tawera gayi from shallow waters (3–5 m) of Ushuaia Bay, Beagle Channel were investigated. Mean abundance and biomass in the study area were 1091 ± 737 ind. m−2 and 901.83 g SFWM m−2 (shell-free wet mass), respectively. Individual growth was described best by the von Bertalanffy growth model with the parameter values H∞ = 28.03 mm, K = 0.288 year−1, t0 = −0.34 (r2 = 0.83). Annual production of the population was estimated to be 120.45 g SFWM m−2 year−1, corresponding to a production-to-biomass ratio (P/B) of 0.134 year−1. The single negative exponential mortality model does not fit the population mortality pattern, but predation by gastropods (Xymenopsis muriciformis, Trophon geversianus, Natica sp.) appears to be the major cause of mortality. These highly mobile predators together with the comparatively slow growth and low turnover of T. gayi in Ushuaia Bay limit its potential for sustainable commercial exploitation.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Instantaneous growth rates of 0-group European flounder, Platichthys flesus, obtained from two 9-day enclosure experiments were used to assess the relative quality of three eelgrass and three bare sand habitats in the Bay of Aarhus, Denmark. Measurements of temperature, dissolved oxygen, salinity, sediment structure and biomass of benthic macrofauna were made during the experiments. Gut content analysis was performed after retrieval of the fish. Growth rates of flounder at two bare sand sites were higher or equal to growth rates at the three eelgrass sites, and at a muddy bare sand site in both experiments. The variation in gut fullness of flounder between vegetated and bare sand sites followed the pattern observed in growth rates, but diverged from the spatial variation in prey abundance, which indicates that there were differences in the feeding efficiency between sites. Overall, habitat complexity, i.e. the presence or absence of vegetation seems to be the most important factor determining the growth rates and, by corollary, habitat quality for juvenile European flounder in the Bay of Aarhus.
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  • 56
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Published reports describing the normal embryo development of Oryzias latipes Temminck and Schlegel, 1846, have relied on exhaustive descriptive processes supported by a series of detailed line drawn figures representing the ideal condition for each stage. Other attempts to trace its development have reported microphotographic descriptions but either did not follow the entire development or lacked clarity and gave only a vague description. In this report, we provide a complete descriptive in vivo color atlas guide of O. latipes normal embryo development. Using the orange–red strain, intact chorionated eggs were incubated under a 16 h light, 8 h dark cycle at 25 ± 1°C. A total of 39 stages were differentiated, from the unfertilized egg to the 24-h-old fry. Our contribution is such that each stage has been photographed from different perspectives with a description made based on key features for rapid identification. This atlas is a practical tool for in vivo visualization and identification of each stage in O. latipes embryo development useful for further developmental and toxicological studies with this fish. This may as well be of help in the standardization of future protocols in which these early moments in the life of the fish may be involved.
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  • 57
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Cross-species amplifications of microsatellite locus Spl-106, which was originally screened from the genome of shovelnose sturgeon (Scaphirhynchus platorynchus) with a perfect TAGA repeat motif, were carried out in four other species of the genera Acipenser. A total of 34 polymerase chain reaction (PCR) products representing 16 different alleles of this locus was sequenced. Sequence analysis results showed that besides the number changes of repeat units, many mutational events, such as single-base substitutions and various insertion/deletion (indels) occurred not only at species level but also at individual level, even among the different alleles within the same individual. The repeat motifs varied from perfect (TAGA)n array to perfect compound (TAAA)m (GAAA)n and perfect or imperfect compound (TAAA)m (TAGA)n (TAAA)x arrays in different species and different individuals. The evolution dynamics of this locus in sturgeons was inferred in that it may evolve from a single perfect to different perfect or imperfect compounds.
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  • 58
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    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Journal of applied ichthyology 21 (2005), S. 0 
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Rabbitfish are currently an important component of the eastern Mediterranean inshore fishery. This study estimates age and calculates growth of Siganus rivulatus and S. luridus from the Lebanese coastal waters based on otolith analysis and compares results with available data from different geographical locations. Annual periodicity of alternating zones was confirmed for S. rivulatus, in which one opaque and one translucent zone were observed to deposit each year, but not for S. luridus. Both species appear to have a maximum lifespan of 6 years. The von Bertalanffy growth functions were Lt = 318.9(1 − e−0.225(t+1.307)) for S. rivulatus and Lt = 274.9(1 − e−0.330(t+1.039)) for S. luridus and length–weight relationships were estimated as 〈inlineGraphic alt="inline image" href="urn:x-wiley:01758659:JAI619:JAI_619_mu1" location="equation/JAI_619_mu1.gif"/〉 for S. rivulatus and 〈inlineGraphic alt="inline image" href="urn:x-wiley:01758659:JAI619:JAI_619_mu2" location="equation/JAI_619_mu2.gif"/〉 for S. luridus. The rabbitfish catches of the Lebanese fishery consist mainly of individuals which have not reached their first maturity. Rabbitfish from the eastern Mediterranean coastal waters appear to grow rapidly most of the year and are thus able to maintain relatively important population size despite intensive fishing pressure.
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    Journal of applied ichthyology 21 (2005), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fish collections on the cooling water filter screens of the Doel power plant were used to analyse temporal trends in estuarine fish populations of the upper Scheldt estuary, Belgium. Between 1991 and 2001, 62 species shared the estuary, with 15 species present at any one time. Identified were 23 marine stragglers, 16 freshwater species and four diadromous species, but their contribution to the total catch was insignificant. In terms of numbers, marine estuarine opportunists (17 species) mainly occurring as juveniles and estuarine residents (two species) were the dominant life cycle categories. Six fish species contributed to 〉97% of the total catch: three Pomatoschistus species [P. minutus (32%), P. microps (30%) and P. lozanoi (6%)], two Clupeidae [Clupea harengus (16%) and Sprattus sprattus (8%)] and Syngnathus rostellatus (5%). Abundance of almost all species in the estuary was highly seasonal and characterized by pronounced abundance peaks. Ordination of the data showed that variability in the temporal structure of estuarine fish community was first determined by differences in annual recruitment and then by predictable, cyclical patterns of species abundance.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The paper presents the results of investigations on the feeding of bream larvae reared in illuminated cages in a mesotrophic lake. The same species of zooplankton were found in both the lake and the fish cages. Copepoda predominated in abundance and biomass. During the mixed feeding the bream larvae diet consisted of rotifers (45.8%), small cladocerans (41.6%) and phytoplankton (13.6%). According to Ivlev's selectivity indices (E), the most important food species were: Keratella cochlearis (E = +9.94), Kellicotia longispina (E = +0.93), K. quadrata (E = +0.92), Bosmina longirostris (E = +0.92), Trichocerca sp. (E = +0.89), and Chydorus sphaericus (E = +0.88). The total length (TL) of bream larvae consuming their first nauplii and copepodites of Copepoda was 8.9 mm. As bream larvae grew, cladocerans were more often selected than rotifers. Copepods usually made up an insignificant component of the diet. Bream larvae in illuminated cages generally fed at night; their alimentary tracts were filled to the maximum in the period from sunset to sunrise. Indices thereof reached 755.6 at night and 278.0 ? in the daytime.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Early life-stage tests over 21 days were performed with zebrafish (origin group 1), environmentally relevant concentrations of the genotoxicant benzo[a]pyrene (BaP), and the solvent dimethylsulfoxide (DMSO). As survival did not correspond to BaP or DMSO, zebrafish from another origin (group 2) were compared with group 1 fish. The outcome was that survival in group 1 fish varied from 26 to 84%, whereas survival in group 2 fish was at least 94% and not affected by either of the two chemicals. This result is likely to be caused by endogenous factor(s) in group 1 fish. The study supports the need for establishing a reliable zebrafish culture prior to toxicity testing.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Population dynamics of 1482 specimens of painted comber, Serranus scriba (Linnaeus, 1758), caught in the Trogir Bay area (eastern mid-Adriatic) from June 2001 to May 2002 were studied to provide information on age, growth, length–weight relationship, mortality and exploitation rate of the species’ stock. Annuli on otholiths indicated the presence of seven age-classes. Obtained von Bertalanffy growth parameters were: L∞ = 30.36 cm, K = 0.192 year−1 and t0 = −0.366.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Experiments were conducted to evaluate the efficacy of five live organisms (Artemia, Brachionus calyciflorus, Chironomus plumosus, Moina macrocopa and Tubifex sp.) and an artificial diet (40% protein) in the larval rearing of Asian catfish Clarias macrocephalus. The larvae were fed three times daily starting at the onset of exogenous feeding. Results showed that the catfish larvae utilized the live organisms more efficiently than the artificial diet. The Tubifex-fed larvae consistently showed the highest growth rate. In trial 1, length increment (64.9 mm), weight gain (3192 mg) and specific growth rate (13.1%) after 8 weeks of feeding were significantly higher (P 〈 0.05) in catfish larvae given Tubifex than those in all other treatments. In trial 2, length increment after 4 weeks of feeding was highest in larvae fed Tubifex (22.9 mm) although it did not significantly differ from that of larvae given Moina (21.0 mm). However, weight gain of larvae fed Tubifex (253.0 mg) was significantly higher than that of larvae fed Moina (171.6 mg). The specific growth rate was highest for larvae fed Tubifex (15.0%) followed by larvae fed Artemia (14.5%), Moina (14.4%) and Chironomus (12.0%). Survival rates of the catfish larvae ranged from 9 to 39% after 8 weeks in trial 1 and from 26 to 83% after 4 weeks in trial 2. The present results suggest that Tubifex is an excellent food and a potential substitute for Artemia in the rearing of catfish larvae.
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    Journal of applied ichthyology 21 (2005), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The stomach contents of 532 adult specimens of the Argentine anchovy, Engraulis anchoita, caught in coastal waters of the Argentine Sea from 1994 to 1996, were analyzed. Larger amounts of food were found in the stomachs of anchovies collected in the northern surveys (35–40°S), with the highest values attained in 1994. Main diet items were copepods, appendicularians, cladocerans, fish eggs, and pteropods. Anchovies caught in the southern surveys (40–45°S) ingested less food, whereby the main items were copepods and appendicularians. Plankton samples collected simultaneously with the fishing trawls were also analyzed. Except for Fritillaria borealis, which occurred only in plankton samples, the same appendicularian species (Oikopleura dioica and O. fusiformis) were found in both stomach contents and in plankton samples. The Ivlev selectivity index calculated for O. dioica and O. fusiformis and for the different maturity stages of both species were in all cases ∼0, supporting the hypothesis of a non-selective feeding.
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    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Endocrine disruption and the feminization of male barbel Barbus barbus was examined during a 4-year investigation in the River Jihlava (Danube basin, Czech Republic) that consisted of temporal monitoring of sexual status in 993 tagged barbel and histological verification of sex change in 149 dissected specimens. In 343 recaptured barbel, six specimens had altered (protandric) gamete production, which is a rather exceptional phenomenon (2.8% of the total number of recaptured original tagged males) and does not explain the unbalanced (biased) sexual and size structure of barbel populations that is often observed. Histological gonadal examination revealed intersexuality to be sporadic (2.0%) and more frequent (14.8%) in river stretches of relatively lower and greater organic pollution loads, respectively. The screening of vitellogenin (Vtg) in blood plasma revealed elevated, strongly fluctuating concentrations in males, unrelated to fish size or age, which reached a median of 0.190 μg ml−1 in the moderately polluted stretches, whereas a significantly higher value of 0.732 μg ml−1 was observed in strongly polluted stream stretches. These levels were apparently a result of elevated levels of ambient xenoestrogens.
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To examine the role of humans in the non-native fish introductions, we measured the frequency of occurrence and density of non-native fishes in ponds (Epping Forest, Essex, England) that had been restored (drained of water and voided of fish or treated with rotenone) on a known date and into which no piscivorous or non-native fishes had subsequently been stocked intentionally. For each pond, the period of time since pond restoration, pond area, distance to nearest residential housing, distance to nearest footpath, distance to nearest water body or stream, and the proportion of pond vegetated were measured. The occurrence of both non-native and unexpected native fish species was non-random, and the number of ornamental varieties was found to increase as pond distance from the nearest road decreased. Variety richness of each of three categories of fish (non-native, goldfish Carassius auratus and native) was significantly correlated with at least two of the following variables: distance from nearest road, nearest footpath and nearest pond. The rate of non-native fish introductions (adjusted variety richness per year) could also be estimated from pond distance to the nearest road, being about 3.5 ornamental varieties introduced per year in ponds adjacent to roads, but the rate appears to be much greater in ponds that had recently (〈1.5 years) undergone restoration. Implications for conservation and management, as well as the potential role of societal issues such as recreational activities, cultural and religious practices, are discussed.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The arrival of non-native fishes in the Levant Basin began in the late 19th century. Whereas the presence of most of the 40 non-native freshwater fishes stem from intentional introductions, either for aquaculture or pest control, the 62 species of non-native marine fishes arrived by natural dispersal via the Suez Canal. Of the non-native freshwater species, five have established successful breeding populations (mosquitofish Gambusia affinis, common carp Cyprinus carpio, crucian carp Carassius carassius, swordtail Xiphophorus hellerii and rainbow trout Oncorhynchus mykiss), and seven are regularly stocked in natural habitats (thinlip mullet Liza ramada, flathead mullet Mugil cephalus, European eel Anguilla anguilla, grass carp Ctenopharyngodon idella, Asian silver carp Hypophthalmichthys molitrix, bighead carp Aristichthys nobilis, black carp Mylopharyngodon piceus). Some non-native species appear to have out-competed native species. Gambusia affinis may have caused the extirpation of two native cyprinid fishes from the Qishon River basin (Levant silver carp Hemigrammocapoeta nana and common garra Garra rufa) and the southern Dead Sea (endemic Sodom's garra G. ghoerensis). The opening of the Suez Canal in 1869 allowed entry into the eastern Mediterranean of Indo-Pacific and Erythrean biota, with the latter now dominating the community structure (50–90% of fish biomass) and function (altered native food web) of the Levantine littoral and infra-littoral zones. The process has accelerated in recent years concurrent with a warming trend of the seawater. Record numbers of newly discovered non-native species is leading to the creation of a human-assisted Erythrean biotic province in the eastern Mediterranean.
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  • 71
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Initial studies on the reproductive parameters and spawning behaviour of racer goby Neogobius gymnotrachelus, one of three Ponto-Caspian gobiid species recently invading Polish waters, were carried out in Włocławski Reservoir (lower River Vistula, Poland), where spawning lasted from April to August. Males were on average about 23.3% longer and 53.8% heavier than females. The smallest female with mature gonads was 58 mm TL (estimated age 2+ years), and males were sexually mature at 59 mm TL (age of 1+ years). Gonado-somatic index at the beginning of spawning was seven times larger in females (mean = 8.4) than that in males (mean = 1.2). Mean absolute fecundity was 952 eggs per female (361–2236). Egg diameter ranged from 0.140 to 1.558 mm, being of bi-modal distribution, which suggests two or three spawning bouts. Length–frequency analysis of juveniles revealed a multimodal distribution. Specialized reproductive behaviour (nest construction and parental care of eggs) and an extended spawning period increase the probability that racer goby can successfully establish self-sustaining populations in novel environments.
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  • 72
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In the previous decade, four species of non-native goby have invaded the middle section of the River Danube. To understand the problems associated with these invasions better, we examined the external morphology of 184 round goby Neogobius melanostomus specimens using both triple regression (distance-based measurements) and geometrical (coordinates-based measurements) analysis within an ontogenetical and epigenetical aspect. Using geometrical analysis of distance-based characters, we found that the external body shape in four size groups of round goby remained essentially unchanged. Such direct development represents a strongly precocial (i.e. specialized) life-history, whereas earlier maturation in this non-native population suggests a shift back towards more altricial (i.e. less specialized, more generalized) life history. This combination of altricial–precocial trajectories seems to be typical for invasive round gobies and may be one of key factors for their successful colonization of new environments.
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  • 73
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ladigesocypris ghigii (Gianferrari, 1927), commonly called gizani, is an endangered freshwater fish endemic to the Greek island of Rhodes. The spawning behaviour of gizani was studied in aquaria, to develop an artificial breeding technique for this endangered species. Spawning in captivity was recorded over 41 spawning days between 15 February and 14 July 2001, under constant temperature (18 or 21°C) and photoperiod 14L/10D. The number of spawning acts in a day was 82 ± 28. No aggressiveness and no territoriality were developed. The mating system of the fish was polygamous, with females spawning more than once. In captivity, eggs are laid either on aquatic plants or on gravel, while in nature fish spawn mainly on algae and plants. The latter suggests that, as far as breeding substrate is concerned, gizani is an opportunistic species using different substrates when necessary.
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  • 74
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The aim of this study was determination of metabolical changes in neutrophil granulocytes of common carp (Cyprinus carpio L.) after acute exposure to cadmium chloride. Cytochemical examination of neutrophil granulocytes was performed on 20 control and 20 experimental specimens of 1- to 2-year-old common carp after 96 h of exposure to cadmium chloride in a concentration of 12.5 mg L−1. We detected an increase of glycogen and lipid content and a decrease of peroxidase and alkaline phosphatase content in neutrophil granulocytes of the experimental group of carp when compared with the control group.
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  • 75
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The Illex illecebrosus fishery in the northwestern Atlantic Ocean is a trawl-based fishery. Illex illecebrosus normally lives 〈1 year, thus managing the fishery using a standard stock assessment approach is difficult. Real-time management is an attractive option. The purpose of this study was to investigate the utility of data on size composition reported by commercial vessels for application in real-time management in the I. illecebrosus fishery. Observations were collected from fish processors for 1996–98 and from fishing vessels directly reporting in 1999–2001. Mean size of I. illecebrosus increased more or less steadily through the fishing season in each year. Apparent growth rates, determined by evaluating the change in mean size in the catch, varied considerably between years. Apparent growth rates appear to be reasonable expressions of true growth rates because either the bulk of the squid entered the fishery at the beginning of the fishery and remained with the fishery for the duration of the season, or the squid on the outer shelf, available to the fishery, represented an emigrated portion of a larger coherent offshore population. Depth exerted only a minor influence on the size of squid caught, whereas the size–frequency varied significantly with latitude. Largest squid were caught at the southern and northern extremes of the study domain. Refrigerated seawater (RSW) trawlers consistently caught smaller squid than did freezer trawlers. The complexity of I. illecebrosus size structure revealed by this study indicates the necessity of obtaining a dispersed data set, in space, with a degree of temporal detail to elucidate trends. Such a data set is conceivably obtainable from fishing vessels reporting information as they fish providing that the sampling design addresses issues concerning the dispersion of vessels across latitude, the appropriate composition of the reporting fleet, and the collection of sufficient observations per tow to adequately resolve the range in size as well as the mean.
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  • 76
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In recent years, much progress has been made in the rearing of fish larvae fed only artificial diets. A preliminary study was made in an attempt to evaluate the effects of live food and formulated diets on survival, growth and body protein content of first-feeding larvae of Plelteobagrus fulvidraco. Three test diets varying in protein level were formulated: Feed 1 containing 45% protein, Feed 2 with 50% protein and Feed 3 with 55% protein. Larvae fed live food (newly hatched Artemia, unenriched) were the control. The experiment started 3 days post-hatch and lasted for 23 days. At the end of the 23-day trial, survival was best in the control group (65.6%) whereby the final body weight and specific growth rate (SGR) were significantly lower than those in the test feed groups. At the same time, coefficients of variation for SGR and final body weight in the test groups were significantly higher than those in the control. Whole body protein content in all treatments showed a similar tendency during development: significantly higher 3 days post-hatch, then decreasing significantly, and then increasing unstatistically 10 days post-hatch. All results suggest that live food is still better for first-feeding larvae of P. fulvidraco, since live food leads to healthier larvae growth.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A microsatellite locus, MFW1, originating from common carp is highly conserved in flanking nucleotides but variable in repeat length in some fishes from different families of the Cypriniformes. This orthologous locus is polymorphic in approximately 58% of the species tested in the order and is inherited by Mendelian law. It proved to be a potentially good marker in population genetics and in the cyprinid species-breeding programme in which no microsatellite markers were available.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Lake sturgeon larval drift is not uniform in time or space and subsequent efforts to determine the relative abundance have suffered because of the lack of information during this early life history period. The purpose of this study was to obtain information about the early life history of lake sturgeon, determine the extent and duration of lake sturgeon larval drift, and examine this relationship to water flow and temperature in the Upper Black River, Michigan. This study also compares the results of other studies to further evaluate the dispersion of larvae. Larval production was quantified using drift nets anchored to the stream bottom from May to June in 2000–2002. Larval drift nets captured 780 larvae in 2000; 2975 larvae in 2001; and 2041 larvae in 2002. For the 2000, 2001, and 2002 spawning season, we estimated that 7107 (95% CL: ± 1470), 17 409 (95% CL: ± 5163), and 15 820 (95% CL: ± 3168) larval lake sturgeon were produced in the Upper Black River (UBR), respectively. Catch per unit effort values of drifting larvae were greatest after peak water flows, with most larvae captured in the middle of the river channel. A mean daily water temperature above 16°C was an important environmental stimulus that influenced peak larval dispersion away from spawning sites. The results of this study suggested that natural reproduction was still occurring in the Black Lake system.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three types of bilateral asymmetry [antisymmetry (AS), directional asymmetry (DA) and fluctuating asymmetry (FA)] have long been recognized in the literature. It is often assumed that DA and AS have a larger genetic basis than FA, which therefore provides a measure of developmental stability. To examine changes in bilateral asymmetry of the Lusitanian toadfish, Halobatrachus didactylus (Bloch & Schneider, 1801), collected in 10 localities along the Portuguese coast, the distribution of left–right values of three external and two internal morphometric variables and one meristic variable were evaluated for normality and deviations of the mean to 0; kurtosis and skewness were also determined. AS was found for the majority of variables in all locations, although DA was also very frequent. FA was observed in only four locations for three variables. A general pattern of negative and low Spearman correlations between asymmetry levels and concentrations of heavy metals and pollutants and mean heterozygosity was found. However, exceptions to this pattern indicate that different asymmetry types can occur across the distribution range in a single trait and that the three types of asymmetry are dynamically interrelated, the shifts from FA to the other two types possibly being due to environmental stress.
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three groups of Oreochromis mossambicus, progeny of a single couple, acclimated to freshwater, seawater and iso-osmotic water, respectively, were confined for 4 h following the same experimental procedures. Confinement stress evoked a ‘simple’ stress response that produced a significant rise in cortisol and glucose and slight osmotic disturbances for each tested salinity. It was speculated that the mechanisms securing osmotic homeostasis in this extremely euryhaline species favour it from a wide osmotic stress-evoked disturbance.
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  • 81
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Amplified fragment length polymorphism (AFLP) was used to analyse the genetic structure of 45 individuals of Gymnocypris przewalskii (Kessler, 1876), an endangered and state-protected rare fish species, from three areas [the Heima (HM), Buha (BH) and Shaliu rivers (SL), all draining into Qinghai Lake]. A total of 563 polymorphic loci were detected. The HM, BH and SL populations have 435, 433 and 391 loci, respectively (Zhu and Wu, 1975), which account for 77.26%, 76.91% and 69.45% of the total number of polymorphic loci of each population, respectively. The Nei indices of genetic diversities (H) of the three populations were calculated to be 0.2869 (HM), 0.2884 (BH) and 0.2663 (SL), respectively. Their Shannon informative indices are 0.4244, 0.4251 and 0.3915, respectively. Research results show that the mean genetic distance between HM and BH is the smallest (0.0511), between BH and SL is the second shortest (0.0608), and between HM and SL is the largest (0.0713), with the mean genetic distance among the three populations being over 0.05. Data mentioned above indicate that the three populations have a certain genetic differentiation. The total genetic diversity (Ht = 0.3045) and the mean value of genetic diversity within the population (Hs = 0.2786) indicate that the variations have mainly come from within the population.
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  • 82
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A survey of three mitochondrial DNA regions (control region, NADH5, cytochrome b) and comprehensive sequencing of the control region (631–646 bps) was conducted to examine whether subspecies and geographic populations within three species of Eurasian sturgeons, Acipenser gueldenstaedtii, A. stellatus, and Huso huso, are genetically distinct. Neither subspecies nor populations exhibited diagnostic distinction or reciprocal monophyly in any gene region examined. For the control region, molecular variance analyses (amova) indicate that most of the variance is because of differences among haplotypes within subspecies (H. huso: 99.6%; A. stellatus: 95.0%; A. gueldenstaedtii: 81.0%) and populations (A. gueldenstaedtii: 76.1%). Significant pairwise F-values were found for all pairwise comparisons except for Sea of Azov and Caspian Sea A. gueldenstaedtii and Caspian Sea and Black Sea A. stellatus and H. huso. Only weak genetic differentiation is apparent between select subspecies and populations, reflective of biogeographic and management history. High genetic diversity within A. gueldenstaedtii suggests the possibility of additional population structure. Future research and management projects should consider these results.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Gymnocharacinus bergi (Pisces, Characidae), an endemic fish in the headwaters of the Valcheta stream in northern Patagonia, displays a fright reaction. As this species inhabits a rapid stream, we hypothesized that the alarm reaction might be socially transmitted through visual information, because the detection of alarm pheromone would be restricted to those fish located downstream from the releaser. Alarm substances elicited a decrease in the swimming activity and a tendency to remain at the bottom of the aquarium. Individuals that observed the fright reaction, but were not exposed to the pheromone, spent significantly more time at the bottom of the aquarium imitating the alarm response, and tended to approach the others. Gregarious behaviour was observed; however, interactions (number of approaches) were stronger between those fish held prior to testing in the same aquarium; smaller fish in particular tended to get closer to bigger ones. Greater group cohesion was found between individuals that were exposed to the alarm pheromone.
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  • 84
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Spatial, seasonal and diel distribution patterns were analysed over a 2-year period for Diapterus auratus and Eucinostomus melanopterus in Pueblo Viejo lagoon, a tropical coastal lagoon in Veracruz, Mexico. A total of 216 samples were collected with 329 individuals of D. auratus and 170 of E. melanopterus. Diapterus auratus individuals were captured throughout a wider range of environmental conditions, and consequently its spatial niche breadth was significantly greater (P 〈 0.001) than that of E. melanopterus. In general, more individuals were captured at localities with submerged vegetation (Ruppia maritima), with 70% for D. auratus and 87.2% for E. melanopterus, although only the latter species showed significant spatial differences. A spatial segregation influenced by distance from the lagoon inlet and salinity was observed between the species. It is considered that this segregation may allow resource partitioning and minimize interspecific competition. A relatively low niche overlap (4.9%) was observed for the two species. Seasonal capture peaks of D. auratus were related to rainfall and the productivity pattern in the lagoon system. Seasonal fluctuations in monthly mean number of D. auratus were correlated with preceding variations in local rainfall (r = 0.86, P 〈 0.02). Moreover, seasonal capture peaks of D. auratus coincided with ecosystem primary production peaks. Although E. melanopterus exhibited no significant seasonal differences, its maximum numbers also coincided with an ecosystem primary production peak and recruitment patterns. In 24-h cycle analysis, both species were captured mainly around mid-day, with minor peaks at dawn and/or at dusk. Generally, few individuals were collected during hours of darkness. It was proposed that diel abundance variation of gerreids might be related to both light penetration in the water column and feeding activity. Food availability within Pueblo Viejo lagoon appears to be an important factor influencing fish abundance.
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  • 85
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The aim of the present study was to investigate possible stressful effects on European sea bass Dicentrarchus labrax reared under constant darkness (0L-24D) and to examine the possible anti-stressful effect of dietary tryptophan (TRP) supplementation. Juvenile European sea bass (initial body weight 4.23 ± 0.032 g) were reared for 10 weeks under 0L-24D and 12L-12D and fed either a commercial diet (0.47% TRP) or the same diet supplemented with L-TRP (2.47% TRP). Results showed that lighting conditions had no significant effect on fish growth, while a depressive effect by the TRP supplementation was obvious. All fish populations reared under 0L-24D exhibited reduced body protein, lipid and ash content and increased food consumption. Reduced body lipids, food consumption and nutrient utilization were observed in TRP-supplemented fed fish, along with lower liver lipids. Dietary TRP enrichment significantly lowered liver saturated and monounsaturated acids and increased poly- and highly-unsaturated fatty acids, especially in combination with 0L-24D. These changes were also considerably reflected in carcass fatty acid composition.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The non-specific immune parameters are useful to determine the health status of fish and to evaluate the immunomodulatory substances for fish farming as markers of pollution and disease resistance. Some of the important parameters, viz. superoxide production by neutrophils through nitroblue tetrazolium (NBT) assay; haemagglutination (HA), haemolysin (HLY) and bacterial agglutination titres; myeloperoxidase (MPO), and lysozyme activities, and alternative complement levels in serum of the juveniles of three Indian major carp species (Cirrhinus mrigala, Catla catla and Labeo rohita) were measured to establish their physiological normal range. A wide variation among the individuals within a species in the ranges of the most of the immune parameters was recorded. Significantly higher levels in the mean values of HA, HLY, bacterial agglutination titres; superoxide production by neutrophils in nitroblue tetrazolium assay; serum MPO and lysozyme activities, i.e. 371.20, 4.60, 18.80, 0.40, 0.62 and 6.55 μg ml−1, respectively, were obtained in L. rohita except a much lower alternative haemolytic complement activity (29.06 units ml−1) compared with the other two species. In most of the parameters, L. rohita showed the highest value, possibly indicating its more natural resistance compared with the other two species.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A rice–fish culture experiment was conducted to determine the effects of different fertilizer treatments on water quality parameters, i.e. dissolved oxygen, temperature, pH and conductivity, as well as the nutrients nitrogen, potassium and phosphorus. Data gathered were subjected to analysis of variance. Dissolved oxygen, nitrogen and potassium concentrations showed significant differences (P〈0.5) among the various rice-cum-fish management systems, with the highest mean concentration in the fish-only management system. The insignificant difference (P〉0.05) in the oxygen concentration between rice-only and rice-cum-fish management systems was probably due to the narrow spacing of rice cultivars (20 × 20 cm). Under different fertilizer treatments, dissolved oxygen, nitrogen and potassium concentrations were not significantly different (P〉0.05), indicating that different fertilizer treatments had no effect on their concentrations. Mean temperature, pH, conductivity and phosphorus concentrations in all rice-cum-fish management systems and fertilizer treatments did not differ significantly (P〉0.05) from each other. This might possibly be due to the hydrogeology and climatic conditions of the area. However, all levels of studied water quality parameters were tolerable to the fish (Oreochromis niloticus) in the rice–fish ecosystem. There was no significant difference (P〉0.05) in rice yield between the rice–fish management system and the rice-only management system. However, there was a significant difference (P〈0.05) under different fertilizer treatments, with nitrogen/phosphorus/potassium (NPK) (20 : 10 : 10) giving the highest production of an estimated 14.36 kg ha−1 year−1, indicating that fertilizer treatments had an effect on the rice yield. Fish growth was not significantly different (P〉0.05) under different rice–fish management systems, but was significant (P〈0.05) under different fertilizer treatments. Chicken manure gave the best growth with 17.7±5.97 g; estimated fish yields were 343 and 602 kg ha−1 year−1 in fish monoculture and rice–fish culture respectively. It is recommended that farmers who are more inclined to fish or rice production in integrated rice-cum-fish management systems could use chicken manure and NPK (20 : 10 : 10) respectively.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The capacity of hybrid tilapia Oreochromis mossambicus × O. niloticus [23.2 ± 0.2 g (mean ± SE)] to show compensatory growth was assessed in an 8-week experiment. Fish were deprived of feed for 1, 2 and 4 weeks, and then fed to satiation for 4 weeks; fish fed to satiation during the experiment served as control. Water temperature gradually declined from 28.1 to 25.5°C throughout the experiment. Specific growth rate (SGR) decreased with progressive food deprivation. At the end of deprivation, body weight was lower in the deprived fish than in the control. Fish deprived for 4 weeks exhibited lower contents of lipids and energy in whole body, and higher moisture content and ratio of protein to energy (P/E) than those of the control; they also consumed feed faster than the control when normal feeding was resumed. All deprived fish showed higher food intake (FI) than that of the control during re-alimentation; however, enhanced SGR was only observed in the fish deprived for 4 weeks. There were no significant differences in digestibility of protein and energy, food efficiency (FE) or energy retention efficiency between the control and deprived fish. At the end of re-alimentation, deprived fish failed to catch up in body weight with the control, while content of moisture, lipids and energy, and P/E in whole body of the deprived fish did not significantly differ from that of the control. The results of the experiment revealed that the hybrid tilapia reared in freshwater showed partial capacity for compensatory growth following food deprivation of 4 weeks, and that growth compensation was due mainly to increased FI, rather than to improved FE.
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    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four microsatellites were used to examine the genetic variability of the spawning stocks of Chinese sturgeon, Acipenser sinensis, from the Yangtze River sampled over a 3-year period (1999–2001). Within 60 individuals, a total of 28 alleles were detected over four polymorphic microsatellite loci. The number of alleles per locus ranged from 4 to 15, with an average allele number of 7. The number of genotypes per locus ranged from 6 to 41. The genetic diversity of four microsatellite loci varied from 0.34 to 0.67, with an average value of 0.54. For the four microsatellite loci, the deviation from the Hardy–Weinberg equilibrium was mainly due to null alleles. The mean number of alleles per locus and the mean heterozygosity were lower than the average values known for anadromous fishes. Fish were clustered according to their microsatellite characteristics using an unsupervised ‘Artificial Neural Networks’ method entitled ‘Self-organizing Map’. The results revealed no significant genetic differentiation considering genetic distance among samples collected during different years. Lack of heterogeneity among different annual groups of spawning stocks was explained by the complex age structure (from 8 to 27 years for males and 12 to 35 years for females) of Chinese sturgeon, leading to formulate an hypothesis about the maintenance of genetic diversity and stability in long-lived animals.
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  • 90
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The ovaries of 501 female eastern Atlantic bluefin tuna (Thunnus thynnus Linnaeus, 1758) captured in the Mediterranean Sea from May to September between 1998 and 2004 were analysed histologically. Body size at median sexual maturity (L50) was 103.6 cm fork length (FL), while 100% maturity was reached above 135 cm FL. The age analysis, based on the count of the translucent zones of the first spiniform ray of the first dorsal fin, showed that most of the specimens with FL = L50 were 3 years old while 100% maturity was reached between 4 to 5 years. The reported evidence indicates that for the eastern Atlantic bluefin tuna stock, the size and age of first sexual maturity of females was lower than in the western Atlantic stock.
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  • 91
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The feeding habits of the horse mackerel, Trachurus trachurus, from the central Adriatic Sea were investigated with respect to fish size and season. Stomach contents of 1200 specimens 12.9–37.6 cm total length (TL) taken at monthly intervals (January–December 1995) were analyzed. Of the total number of stomachs examined, 597 were empty (49.7%). This percentage varied significantly with season; the maximum number of empty stomachs was observed during January (80%) and February (82%) and a minimum number observed during July (15%) and August (19%). A total of 30 different prey species belonging to five major groups: Crustacea (Euphausiacea, Mysidacea, Decapoda), Cephalopoda, and Teleostei was identified in the stomach contents. Euphausiids were the most important ingested prey in all seasons as well as in small- and medium-size classes (〈28 cm TL). Two species of euphausiids, Nyctiphanes couchii (%IRI = 36.0) and Euphausia krohni (%IRI = 12.9), were the most frequent prey. The proportion of teleosts in stomach contents increased with the increasing size class of horse mackerel and was 15.5% IRI in 〉30 cm TL individuals. The mean weight of stomach contents increased significantly for fish larger than 24.1 cm TL, while the mean number of prey items significantly declined in the large-size classes.
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  • 92
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Toxicity of four insecticides commonly used in rice pest management, chlorpyrifos, dimethoate, carbaryl and carbosulfan, to the fry of common carp was assessed through median lethal concentrations (LC50) and in vivo inhibition of the brain acetylcholinesterase (AChE) enzyme at sublethal concentrations. The 96-h LC50 values for these four insecticides were determined to be 0.008, 26.11, 7.85 and 0.60 mg L−1 respectively. Exposure of fish to a series of sublethal concentrations (0.5–5% LC50) of each insecticide for 14 days resulted in concentration-dependent inhibition in AChE activity in comparison with the controls. AChE activity was greatly inhibited in the fish exposed to sublethal concentrations of chlorpyrifos. Upon transfer to insecticide-free water, AChE activities in fry exposed to 0.5 and 1% LC50 concentrations of carbaryl and carbosulfan were restored to the control level within 7–21 days whereas the fish exposed to chlorpyrifos or dimethoate did not fully recover from the insecticide-induced anticholinesterase action. Of the four insecticides tested, chlorpyrifos was the most toxic for the fry of common carp. Although dimethoate was least toxic for the fish under acute exposure, the restoration level of normal AChE activity was slower under chronic exposure in comparison with carbaryl and carbosulfan. Hence, the use of carbamates, especially carbaryl, to control insect pests of rice in rice-cum-carp culture systems is recommended when considering survival, restoration of the normal AChE activity and stamina of the cultured fish.
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  • 93
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Formaldehyde is one of the shortlist substances permitted for use in aquaculture. Toxicity of formaldehyde and therapeutical success is influenced by water parameters. However, there is a lack of information on the interaction of organic carbon and calcium (Ca2+) on the toxicity of formaldehyde. Laboratory experiments were conducted to test the combined effects of Ca2+ and natural organic matter (NOM) on formaldehyde toxicity. Zebrafish embryos were exposed to five concentrations of formaldehyde for 144 h. Juveniles exposed to high concentrations died; the embryos exposed to low concentrations were unable to hatch, particularly visible in the groups without NOM. The toxicity-reducing effects of NOM are not attributable to the presence of Ca2+.
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  • 94
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The locations and habitat features of freshwater holding areas and the timing of fall migration are undocumented for Gulf sturgeon in the Pascagoula River drainage, Mississippi. Our objectives were to identify and characterize holding areas for Gulf sturgeon (Acipenser oxyrinchus desotoi), document their summer and fall movement patterns, and determine migration cues. To do this we captured, radio-tagged, and monitored movement of Gulf sturgeon in the Pascagoula River drainage and analyzed these data using geographic information systems. From May to November Gulf sturgeon congregate in a holding area in the lower portion of the Pascagoula River and Big Black Creek [river kilometers (rkm) 57–68] and near Cumbest Bluff (rkm 40), before they return to the Gulf of Mexico. While in the holding area, Gulf sturgeon were typically found in deep locations, either in or downstream from river bends. As found in other rivers, Gulf sturgeon in the Pascagoula River showed little movement within the holding area and often stayed within a single river bend; although we observed local movements by some individuals (under 10 rkm). In the Pascagoula River, Gulf sturgeon initiated their migration out of fresh water from late-September to mid-October, coincident with shorter day length, falling water temperature (mean = 23.7°C, range 21–26°C), and elevated river flow. Our work demonstrates that the lower Pascagoula River serves as a vital area for Gulf sturgeon.
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  • 95
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Common carp, Cyprinus carpio (40 ± 10 g) were inoculated through intramuscular route (2.5 × 105 cfu ml−1) with the fungal pathogen, Aphanomyces invadans. In the infected group, the total erythrocyte count (RBC), total leucocyte count (WBC), haematocrit (Hct), haemoglobin (Hb), lymphocyte (LYM), monocyte (MON), neutrophil (NEU) and eosinophil (EOS) deviated significantly (P 〈 0.05) from control values on days 24 and 36. Beginning on day 12 of infection, the fishes were dip treated for 24 days for 5 min daily in water incorporated with 1% aqueous leaf extract of Azadirachta indica (neem); the induced lesion gradually healed and the haematological values attained were near normal (P 〉 0.05) when compared with the control group.
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  • 96
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 97
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Throughout Europe in the last decade there has been a steady shift away from more technical fish pass designs to more nature-like passes, such as nature-like bypass channels. Upstream fish passage in a nature-like bypass channel was investigated in a lowland river, the Lima River, for 117 days from March 2000 to May 2002. Fish passage was recorded using an automatic video recording system. Electrofishing samples within the bypass and below the weir were compared with species abundance found on the tape recordings. More than 7500 individuals of eight species passed through the bypass channel. Species composition was dominated by striped mullet (65.3%) and potamodromous species (34.3%), which used the bypass mainly at night. Of the environmental variables considered, bypass discharge explained most of the variation in the number of cyprinids, whereas water temperature was more important for diadromous species. Comparing species composition below the weir using passage recordings provided a useful tool to assess species efficacy of the bypass, although biological requirements should also be taken into account. This study proved the efficacy of the bypass for passage of almost all occurring species and life stages and also for providing suitable habitat for fish fauna, highlighting the use of these facilities for river restoration schemes.
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  • 98
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    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A rapid, reproducible method is described for extracting and comparing levels of the ether-soluble fish androgen 11-ketotestosterone (11-KT) in blood serum, muscle tissue and surface mucus. Because widely different volumes of extract were recovered after centrifugation from the three sources, it was important to express androgen levels as pg 11-KT/mg of total soluble protein (TSP). For six male and four female sexually-staged freshwater Koi (Cyprinus carpio), the method yielded similar pg 11-KT/mg TSP ratios in blood serum and extracts of muscle tissue and surface mucus, with the strongest correlation between blood serum and surface mucus. While male Koi were distinguishable from females based on the magnitude of 11-KT levels, reproductive stage and gonadosomatic index levels were not correlated with the 11-KT levels of either sex. Similar pg 11-KT/mg TSP ratios were also found for autologous muscle tissue and surface mucus extracts of 37 captured and sexed wild marine fishes representing seven genera. However, high 11-KT levels were not restricted to mature males. Collectively, results suggest that surface mucus collection (followed by 11-KT assay) is a useful alternative to more invasive methods of determining systemic hormone levels in fish. Without knowledge of seasonal variation in levels of this and other sex hormones, however, reliance on 11-KT levels alone may lead to spurious identification of gender, let alone reproductive stage.
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  • 99
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The primary objective of this study was to identify and characterize juvenile white sturgeon (Acipenser transmontanus) habitat in the Lower Fraser River downstream of Hope, BC, Canada. A secondary objective was to estimate the juvenile white sturgeon population in the Lower Fraser River. A total of 1867 white sturgeon was captured with gill nets at 26 sites in the Lower Fraser River. The greatest numbers of sturgeon were caught in three sloughs; all but three sturgeon were captured in the June to August period. These three sloughs all had water deeper than 5 m and current that was multidirectional. Turbidity ranged greatly from 1.5 NTU (Nephelometric Units) to 67.0 NTU and the substrate of most sites was fine sediments, fine sand, silt and clay. Stomach contents were mysid shrimp (Mysidacea), midge larvae (Chironomidae) and peamouth chub (Mylocheilus caurinus). We identified three of 26 sites with appreciable numbers of juvenile sturgeon, identified water quality parameters of these sites, identified any incidental species that might be prey and also determined that between 1985 and 1993 the juvenile white sturgeon population had declined.
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  • 100
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    Berlin, Germany : Blackwell Verlag GmbH
    Journal of applied ichthyology 21 (2005), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Zander (Sander lucioperca) is one of the most important fish species in the German inland fishery. As a pelagic predator, zander is able to regulate populations of small cyprinids and perch and is therefore used in ‘top-down’ projects to directly control the food web. At present, natural waters are mainly stocked with one-summer-old zander from pond hatcheries; however, such fish are poor stocking material due to low survival rates, presumably because of their small size. Two groups of 50-day-old zander fingerlings reared in a recirculation system (T = 23 °C) were used. For 90 days one group (FG 1) was fed with a commercial dry feed (Trouvit Pro Aqua Brut), and the other group (FG 2) was fed with chironomides (Chironomus spp.). The fat content of the dry feed was increased with fish oil supplemented up to 22%. After the rearing period the FG 1 fishes attained the largest size and fat content. A pond with a surface of 667 m2 and a mean depth of 0.8 m was stocked with 24-tagged zander (12 of each feeding group) for wintering. After the winter period of 176 days, survival rate of each feeding group was 83.3%. The fatty acid composition in the membrane lipids of group 2 changed dramatically. In contrast to previous investigations, presented results indicate that the survival rate is not influenced by fish size (weight and length). The crucial factors for high survival rates of zander during wintering are the crude tissue fat content (〉5%) and its fatty acid composition.
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