Publikationsdatum:
1998-06-11
Beschreibung:
The mouse Clock gene encodes a bHLH-PAS protein that regulates circadian rhythms and is related to transcription factors that act as heterodimers. Potential partners of CLOCK were isolated in a two-hybrid screen, and one, BMAL1, was coexpressed with CLOCK and PER1 at known circadian clock sites in brain and retina. CLOCK-BMAL1 heterodimers activated transcription from E-box elements, a type of transcription factor-binding site, found adjacent to the mouse per1 gene and from an identical E-box known to be important for per gene expression in Drosophila. Mutant CLOCK from the dominant-negative Clock allele and BMAL1 formed heterodimers that bound DNA but failed to activate transcription. Thus, CLOCK-BMAL1 heterodimers appear to drive the positive component of per transcriptional oscillations, which are thought to underlie circadian rhythmicity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gekakis, N -- Staknis, D -- Nguyen, H B -- Davis, F C -- Wilsbacher, L D -- King, D P -- Takahashi, J S -- Weitz, C J -- New York, N.Y. -- Science. 1998 Jun 5;280(5369):1564-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neurobiology, Harvard Medical School, Boston MA 02115, USA. 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9616112" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
ARNTL Transcription Factors
;
Animals
;
Basic Helix-Loop-Helix Transcription Factors
;
Biological Clocks
;
CLOCK Proteins
;
Cell Cycle Proteins
;
Circadian Rhythm/genetics/*physiology
;
Cloning, Molecular
;
Cricetinae
;
DNA/metabolism
;
Dimerization
;
Feedback
;
Gene Expression
;
Helix-Loop-Helix Motifs
;
Male
;
Mesocricetus
;
Mice
;
Mutation
;
Nuclear Proteins/*genetics/metabolism
;
Period Circadian Proteins
;
Promoter Regions, Genetic
;
Retina/metabolism
;
Suprachiasmatic Nucleus/metabolism
;
Trans-Activators/genetics/*metabolism
;
Transcription Factors/genetics/*metabolism
;
*Transcriptional Activation
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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