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  • Articles  (55)
  • 21
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    Investigation on genetic structure of Cobia (Rachycentron canadum) using Microsatellite Markers (2018)
    Details
    Publication Date: 2021-05-19
    Description: The genetic diversity of Cobia, Rachycentron canadum populations in the Persian Gulf and Oman Sea were assessed using microsatellite technique. We removed about 3-5g of pectoral and dorsal fin tissue from 184 samples in winter 2006 and spring 2007, and stored it in pure ethylic alcohol (96%). Polymerase chain reactions (PCR) were conducted on the target DNA using 10 paired microsatellite primers. The dendrogram was constructed and drawn using MEGA software package version 4. Based on the analysis of molecular variance, the highest Fst (0.063) was observed when comparing specimens from Dayer Port and Pozm zones. Significant differences (P〈0.01) were not observed between R5t recorded for the specimens studied in the same region but were observed between RSA recorded for different regions. The dendrogram of genetic distance showed two major clusters: the Bushehr and Dayer populations were in one cluster, and the remaining four populations in the other. The second cluster was further separated into two sub-clusters: the Lengeh and Bandar Abbas populations composed one cluster and the Pozm and the Beris populations were in the other cluster. The present study showed that at least three different populations of R. canadum are living in the Persian Gulf and Oman Sea. The populations include Bushehr, Bandar Abbas and Chabahar populations.
    Description: Published
    Keywords: Fins ; Primers ; Rachycentron canadum ; Population genetics ; DNA ; Genetic diversity ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.67-78
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  • 22
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    Genetic variation of Barbus Capito in the southern Caspian Sea by PCR-RFLP method (2018)
    Details
    Publication Date: 2021-05-19
    Description: In this study 60 samples were collected from the southern Caspian Sea and some rivers of Mazandaran and Guilan provinces. Genetic variation and probable population differentiation of Barbus capito were studied based on the mitochondrial cytochrom-b gene. The mitochondrial DNA was extracted from fish fin using phenol-chlorophorm method. The specific primers were designed for B. capito and the PCR experiments were done on 60 samples. 11 restriction endonuclease enzymes were applied for RFLP analysis (ALuI, AvaI, AvaII, HinfI, DdeI, HincI, HpaII, RsaI, Sau3AI, HaeIII, TaqI). PCR products (1062 pb) and DNA digests were subjected to agarose and polyacrilamid gel electrophoresis to seperate fragments according to their molecular weight. These patterns were identified similar fpr all samples. Regarding to this patterns, it can be seen that polymorphysm phenomena cannot be observed by above mentioned enzymes and cytochrome-b gene, and there is no seperate population of B. capito in the southern Caspian Sea.
    Description: Published
    Keywords: mtDNA ; RFLP ; PCR ; Barbus capito ; Genetic variation ; Samples ; Mitochondrial cytochrom-b ; DNA ; Phenol-chlorophorm ; ALuI ; AvaI ; AvaII ; HinfI ; DdeI ; B. capito
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.117-130
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  • 23
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    Population genetic molecular study of Penaeus semisulcatus from Persian Gulf and Oman Sea by using of Cytochrome oxidized COI and RFLP method (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Goals: Determine of barcode of DNA in green tiger prawn, Penaeus semisulcatus, in the Gen bank of the species. Material and methods: In these study 30 specimens of Penaeus semisulcatus from each region in the Persian Gulf and Sea of Oman were sampled and preserved in ethanol 96%. The total DNA was extracted, COI gene was first amplified and then sequenced for each species. Finally the collected data were analyzed with the specific phylogenetic software. Result and discussion: Molecular analysis revealed some degree of interpopulation differences within two areas. Also for population study molecular data of species Penaeus semisulcatus were analysed base on COI RFLP and 16SrRNA sequences respectively. The results indicated that COI gen is a good marker for shrimp species differentiation that would be helpful to protect shrimp species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Genetic ; Molecular ; Population ; Cytochrome oxidized COI ; Penaeus semisulcatus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 24pp.
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  • 24
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    Characterization and isolation of microsatellite in Persian sturgeon (Acipenser persicus, Borodine, 1897) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In order to have a sustainable management on Persian sturgeon (Acipenser persicus) as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Persian sturgeon ; Acipenser persicus ; Microsatellite ; Population ; Genetic ; Species ; Diversity ; Russian Sturgeon ; A. gueldenstaedtii ; A. stellatus ; A.nudiventris
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 160pp.
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  • 25
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    An investigation on possibility of Nile Tilapia (Oreochromis niloticus) feminization under the condition of brackish water in Bafgh (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was aimed to investigate the effect of different doses of oral diethylstilbestrol (DES) on feminization of Nile tilapia (Oreochromis niloticus) larvae under the condition of brackish water in Bafgh fisheries research center. This Experiment was comprised of two control groups in two replicates and two experimental ones in three replicates. At the beginning of the trial, a number of 2150 larvae were randomly distributed among 10 replicates that were under treatment for 45 days. The diets in two control groups, i.e. non-treated, C1, and ethanol treated feed, C2, and the ones in two experimental groups, i.e. 200 and 400 mg DES/k of feed, E1 and E2, were administrated to 7 day post fertilization (dpf) larvae for a period of 35 days and then until day 45, the larvae were fed with ordinary feed. At the end of the trial, the gender ratio of larvae in different groups was determined by squash test in 50 randomly selected individuals from each replicate. The results showed no significant differences for gender ratios within control groups (respective male to female ratio in C1 and C2 was 47:53 and 57:43). However, the corresponding ratios within experimental ones, fed with 200 and 400 mg DES/k of feed respectively, was significantly different (P〈0.05). Noteworthy that the percent of females between two experimental groups (E1 and E2) was not significantly different (70.0 and 64.7 respectively) but it was significantly different with ratios in experimental groups (P〈0.05). The total body length and live body weight were determined at the begging and then at day 30 and 45, it was randomly measured in 20 larvae from each replicate. At the beginning of the experiment, the mean body weight and total length of larvae was 0.012 gram and 1.03 centimeter respectively. The respective averages at day 30 and 45 were significantly different among groups (P〈0.05). Survival rate was significantly different among groups (P〈0.05), being highest in E1 and E2 respectively whereas lowest in control ones (56.51 and 58.84 in C1 and C2 respectively).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Experimental ; Nile tilapia ; Feminization ; Diethylstilbestrol ; Brackish Water ; Oreochromis niloticus ; Fisheries ; Fertilization ; Larvae ; Females ; Survival rate
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 34pp.
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  • 26
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    Introducing of genetic indicators for identification of three commercial shrimps from Persian Gulf and Oman Sea by using PCR-RFLP analysis (2018)
    Details
    Publication Date: 2021-05-19
    Description: Some bottom trawls were carried out in the Persian Gulf and Oman Sea (Hormozgan and Bushehr provinces) regarding to study the main three commercial species shrimp, namely: Penaeus indicus, P. merguiensis and P. semisulcatus. Six samples or each species were collected for further examinations. Total DNA Of each sample was extracted using the phenol and chlorophorm method. mtDNA was ampliphied by using a pair of primers from sequencing of two Of Cytocrom Oxydase unit . The PCR products were digested using nine restriction endonuclease enzyme. Seven enzymes showed polymorphic patterns among different species, and 3 enzymes of Hinf , Hinc  and Rsa  were recognized as genetic markers for species identification or shrimps.
    Description: Published
    Keywords: Commercially ; Genetic ; Shrimp ; PCR-RFLP ; Species ; Penaeus indicus ; P. merguiensis ; P. semisulcatus ; DNA ; Phenol ; Chlorophorm ; Cytocrom Oxydase ; Enzymes ; Identification
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.11-20
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  • 27
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    Genetic relationships of Iranian coastline ship sturgeon (Acipenser nudiventris) samples and Ural population based on microsatellite DNA (2021)
    In:  http://aquaticcommons.org/id/eprint/22458 | 18721 | 2018-04-09 17:47:06 | 22458 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: Genetic relationships among 96 specimens of ship sturgeon, Acipenser nudiventris, collected from six locations along the Iranian coastline as well as 8 specimens from northern population (Ural River) were analysed by the restricted maximum likelihood method, as well as two distance analysis method (Nei's and Cavalli-Sforza's distance) of gene frequencies. We have used four SSR markers that produced three polymorphic and two monomorphic loci. Overall results showed that Iranian coastline samples form a monophyletic group (clade) which was different from the northern (Ural) samples. Iranian coastline samples are divided into two groups (clades): Anzali-Kiashahr clade and Sefidrud-Babolsar-Noushahr-Gorgan clade. Bootstrapping test showed monophyleticity of Sefidrud, Babolsar, Noushahr, Gorgan with high (Bootstrap support=93) and Anzali-Kiashahr with middle (Bootstrap support=65) confirmation. Topology of reconstructed trees was in correspondence with geographical distributions of samples.
    Keywords: Biology ; Fisheries ; Ship sturgeon ; Acipenser nudiventris ; Genetic relationships ; Microsatellite DNA ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 229-240
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  • 28
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    Molecular study of the populations of Artemia partenogenetica in Iran using PCR-RFLP Method (2021)
    In:  http://aquaticcommons.org/id/eprint/23679 | 18721 | 2018-07-18 06:36:39 | 23679 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Considering the importance of genetic studies to manifest inter population differences in species, samples of Artemia partenogenetica were collected from seven inland lakes including Shoor and Inche-Borun lakes in Golestan Province, Hoze-Soltan and Namak lakes in Qom Province, Maharloo and Bakhteghan lakes in Fars Province and Mighan pool in Markazi Province. A total of 210 samples were subjected to DNA extraction by phenol-chloroform method. Primers were designed on a ribosomal fragment (16SrRNA) of the species' mtDNA sequence and the PCR was conducted on the samples. Digestion of the PCR product with approximately 1584bp lengths by 10 restriction endonuclease (AluI, EcoRI, Eco47I, HaeIII, HindIII, HinfI, MboI, MspI, RsaI, TaqI) showed 12 different haplotypes: 4 haplotypes in Shoor and Inche-Borun, 1 in Namak and Hoze-Soltan, 3 in Mighan pool, 1 in Bakhtegan and Maharloo and 3 in Maharloo. Haplotype diversity values within collected samples varied from zero in Hoze-Soltan, Namak and Bakhteghan samples to 0.7425 in Inche-Borun and Shoor while nucleotide diversity varied from zero in Hoze-Soltan, Namak and Bakhteghan, to 0.0077 in Mighan. The minimum nucleotide diversity among samples was zero between Hoze-Soltan vs. Namak and the maximum was 0.1700 between Inche-Borun and Shoor vs. Mighan. Nucleotide divergences among samples were least in Inche-Borun vs. Shoor (%-0.02) and most in Inche-Borun and Shoor vs. Mighan (%16.18), averaging to %3.40. The evolutionary distances between 12 haplotype showed that the maximum value belonged to Mighan haplotypes vs. Inche-Borun and Shoor haplotypes. Regarding the digestive patterns produced by each enzyme in the studied region, Eco47I is introduced as the population-specific marker of A. partenogenetica in Iran. Test of population differentiation based on haplotype frequencies were statistically significant (P≤0.001) with the exception of Hoze-Soltan vs. Namak and Inche-Borun vs. Shoor. We conclude that there are enough evidences in haplotypic level for dividing A. partenogenetica in Iran into five populations: Hoze-Soltan and Namak, Mighan, Maharloo, Bakhtegan, Incheh-Borun and Shoor.
    Keywords: Biology ; Population Genetics ; Genetics ; DNA ; Enzymes ; Marine ; RNA ; Nucleotide sequence ; Primers ; Nucleotides ; Artemia partenogenetica ; ISW ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 53-68
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  • 29
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    Molecular evaluation of the population of Silver Pomfret (Pampus argenteus) in the Persian Gulf and Oman Sea (Iranian &Kuwaiti waters) (2021)
    In:  http://aquaticcommons.org/id/eprint/23749 | 18721 | 2018-07-25 05:46:40 | 23749 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: A total of 162 specimens of Silver Pomfret (Pampus argenteus) including 38 samples from Kuwait and 124 samples from Iran (24 from Khouzestan, 24 from Chabahar and 38 from Bushehr) were examined for their DNA structure. DNA from all specimens was extracted using the Phenol-chloroform and amplified using PCR method with a pair of primers with ND sub(2) gene sequence. The PCR products were about 1300 (bp) for all samples. For RFLP analysis 16 restriction enzymes Bcl I, Pst I, Acc II, BseN I, Tru I, Dra I, Alw26 I, Hin6 I, Tas I, Alu I, EcoR I ,Hae III, Hinc II, Hind III, Hinf I and Hpa II were used. DNA bands were visualized by Gel electrophoresis (polyacrylamid) and staining with silver nitrate. Out of 16 enzymes, four showed polymorphism that includedg Hinf I, Alu I, Acc II, and Hpa II. Of the total 162 samples, 13 showed polymorphic patterns. Six haplotypes were rare occurring only once, but others which were only four different kinds occurred more than once. The REAP test gave no significant result for the examined regions (P〉0.05). Therefore, our results revealed that the Pampus argenteus stocks in the Persian Gulf and Oman Sea of Iranian and Kuwaiti waters constitute a unique population.
    Keywords: Fisheries ; PCR-RFLP ; Pampus argenteus ; Persian Gulf ; Bushehr ISW ; Oman ISW ; Kuwait ISW ; Oman Sea ; Iran ; ISW
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 45-56
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  • 30
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    Identification and extraction of streptococcusis gene from streptococcus iniae and recording in Gene Bank (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25458 | 18721 | 2018-09-26 09:47:40 | 25458 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: S. iniae is an important pathogen in both marin animals and humans, causing systemic infections in these hosts. The symptoms of infections are very similar to symptoms of infections caused by human¬specific streptococcal pathogens. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in s. iniae. We aim to isolate S. iniae and cloning phosphoglucomutase gene. S. iniae grew on sheep blood agar as mucoid and beta- hemolytic colonies after 24 h of incubation at 37C. Then confirmed it by sensitive and rapid method of PCR. The pgm gene was amplified successfully and cloned in pTZ57R cloning vector.The recombinant plasmid was sub cloned into pETDuet-l expression vector by rectriction enzymes and confirmed by PCR. Although S. iniae is a pathogen of economic importance, relatively little is known regarding mechanisms of its pathogenesis and few specific virulence determinants have been established. These include the enzyme phosphoglucomutase which contributes to cell wall integrity and resistance to antimicrobial peptides. Thus, this enzyme is the best choice to product the vaccine and more investigations.
    Keywords: Biology ; Iran ; Streptococcusis ; Gene ; Streptococcus iniae ; Gene Bank ; S. iniae ; Pathogen ; Enzyme ; Phosphoglucomutase ; PCR
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 33
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