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  • epididymal epithelial cells  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Primary culture of ram epididymal epithelial cells (1989)
    Springer
    Methods in cell science 12 (1989), S. 77-81 
    Details
    ISSN: 1573-0603
    Keywords: primary culture ; ram lamb epididymitis ; epididymal epithelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A procedure for the primary culture of epithelial cells harvested from the epididymides of young virgin ram lambs is described. A confluent monolayer of epitheial cells can be produced from cell aggregates isolated from the caput and cauda segments of ram lamb epididymides after trypsin and collagenase digestions. A culture of enriched epididymal epithelial cells is obtained using a selective attachment procedure. Characteristic epithelial cell morphology can be confirmed by phase contrast and indirect immunofluorescent microscopies. Cultured epithelial cells can be passed 4 times and maintained for 1 mo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404648
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