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  • Species  (18)
  • Management
Collection
Keywords
Years
  • 1
    Publication Date: 2021-05-19
    Description: Two water bodies of the Persian Gulf and Oman Sea located in the South of Iran and different vertebrate aquatics inhabiting there consist of fishes (Osteichthyes and Chondricthyes), marine mammals, sea snakes and reptiles. A total of 860 fish species belongs to 31 orders and 142 families have been identified and reported. Amongst them, some important species have been studied from point of molecular genetics and population genetics such as croaker, silver pomfret, cobia, sardines, lanternfishes…. . On the other hand, from marine mammals a total of 10 species of dolphins, 14 species of whales and one species of dugong were identified.Also, 9 species of sea snakes have been reported from the Persian Gulf and Oman Sea belongs to 6 genus and 2 subfamilies of Hydrophidae family. It should be mentioned that no genetic studies have been done on marine mammals and sea snakes till now. In addition, a total of 5 species of sea turtles have been reported from the study area and there are few background on genetic studies for main species in the Persian Gulf.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Diversity ; Distribution ; Species ; Genetic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 46pp.
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  • 2
    Publication Date: 2021-05-19
    Description: The fishery stocks of most commercial aquatic stocks in the world have shown declining trends in the past two decades. Several factors have been responsible for the decline of stocks the most important of which over fishing and over-exploitation, pollution, loss of natural habitats and natural spawning grounds, construction of dams and bridges across the important rivers which restrict the migratory routes of spawners, decrease in natural reproduction and rehabilitation of stocks through artificial breeding programs. Over-exploitation of stocks and pollution directly affect decreasing stocks in an ecosystem. Not differentiating between different populations and stocks of a species found distributed in an aquatic ecosystem is considered one of the main factors which causes the depletion of stocks in most ecosystems in the world. In most cases this is because genetic variations in aquatic stocks in the wild are not taken into consideration. Six species of sturgeons are found living in the Caspian Sea and its drainage basin which produce more than 85-90% of the world caviar. The Persian sturgeon (Acipenser persicus) and the stellate sturgeon (Acipenser stellatus) are the main sturgeon species of the Caspian Sea. The Persian sturgeon is mainly found in the south Caspian Sea while the stellate sturgeon stocks are considered shared stocks by the five Caspian littoral states. Due to over fishing in the past two decades the legal catch figures for sturgeon stocks in the Caspian Sea dropped from 28500 tons in 1985 to less than 1500 tons in 2004. Similarly caviar production also dropped from 3000 tons to 110 tons in 2005. With regard to the severe reduction in sturgeon stocks it is necessary to take essential steps before these valuable species are totally wiped out. The fisheries management of the five Caspian littoral states should focus their efforts on identifying the different populations and stocks found in the Caspian Sea. Concerted measures should be taken to study the distribution and biomass of the different populations in order to develop a scientific solution for the sustainable use of these endangered species and to secure the long term conservation of sturgeon stocks. The aim of present study was to evaluate the genetic structure of the population of two species Acipenser persicus and Acipenser stellatus and to develop molecular markers to identify and differentiate different populations of these two species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Population ; Genetic ; Persian Sturgeon ; Acipencer persicus ; Stellate Sturgeon ; Acipencer stellatus ; Microsatellite markers ; Fishery ; Pollution ; Spawning ; Species ; Aquatic Ecosystem ; Genetic ; Molecular
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 70pp.
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  • 3
    Publication Date: 2021-05-19
    Description: In order to have a sustainable management on Persian sturgeon as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea.
    Description: Published
    Keywords: Russian sturgeon ; A. gueldenstaedtii ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Population genetic ; Genetics ; Population ; Structure ; Species
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.548-558
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  • 4
    Publication Date: 2021-05-19
    Description: The phylogenetic relationship of five sturgeon species in the South Caspian Sea was investigated using mtDNA molecule. Sequence analysis of mtDNA D-loop region of five sturgeon species [Great sturgeon (Huso huso), Russian sturgeon (Acipenser gueldenstaedtii), Persian sturgeon (Acipenser persicus), Ship sturgeon (Acipenser nudiventris), Stellate sturgeon (Acipenser stellatus)] and DNA sequencing of two Escherichia coli cloned DNA fragments sized 758 bp and 922 bp was carried out. Sequence comparison of the mtDNA D-loop region between the five sturgeon species showed 100 % sequence identity. However comparison between a short (758 bp) and long (922bp) mtDNA D-loop region within Russian sturgeon showed a 5 bp differences. On the contrary a partial sequence of the mtDNA ND5 gene region showed high sequence and organic base variability in the five species. These results indicate that direct sequencing of ND5 gene provide more information for phylogenetic or population level studies in sturgeon species. The present study revealed a distinct classification among the five sturgeon species. On the basis of the phylogeny tree it is clear that a close evolutionary relationship exists between the great sturgeon and ship sturgeon that was verified in five phylogenetic trees. On the other hand a close resemblance was also seen between Persian and Russian sturgeon indicates that these two species have a closer evolutionary relationship. This study also showed a 2.2 % sequence divergence in sequence data for ND5 gene between Persian sturgeon and Russian sturgeon which indicated that these two species diverged from each other approximately one million years ago. However the verification of this calls for DNA sequencing of different genes.
    Description: Published
    Keywords: Acipenser nudiventris ; Acipenser stellatus ; Acipenser persicus ; Acipenser gueldenstaedtii ; Huso huso ; Sturgeon ; Phylogenetic ; PCR ; Species
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.1-12
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  • 5
    Publication Date: 2021-05-19
    Description: Karyotype and number of chromosomes of silver carp (Hypophthalmichthys molitrix) were determined by using tissue-squashing method and gimsa staining. In this experiment 80 larvae and 10 fingerlings (weighting 1-8 g) were examined and totally 30 chromosomal slides were prepared. The obtain results indicated that the number of chromosomes in this species was found 2n=48 (with 88 chromosomal arms). Consist of 6 pairs metacentric (M), 14 pairs submetacentric (SM) and 4 pairs of Acrocentric (A). The karyotype formula can be stated as: (6M + 14SM + 4A).
    Description: Published
    Keywords: Karyotyping ; Chromosome ; Silver Carp ; Hypophthalmichthys molitrix ; Tissue-squashing method ; larvae ; Fingerlings ; Species
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.107-115
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  • 6
    Publication Date: 2021-05-19
    Description: Schizothorax zarndnyi is one of the most economic and endemic species, which are found in rivers and triple lagoons of Hamoon. In this study, reproduction cycle, type, season and optimum temperature of spawning period was studied from July 1998 to June 1999. The maximum GSI was 7.9-9.6 in March and April with 14-18°C mean temperatures. The minimum GSI was 0.92 in May. Variation in GSI values shows that reproduction has annual cycle which begins in May and ends in April of the next year. From October significant increase of oocyte diameter and GSI were observed in 2 different groups, while oocyte distribution observed only in one group in May. The rate of HSI had flucatuation, during resting period of ovule (from May to September) and from October simultaneously with significant increase of GSI, the HSI increased and continued to January and decreased in March and April. The condition factor, was changed during reproduction cycle and it was minimum in March (0.92). Concerning these investigation, Sch. zarndnyi is a total spawner and showing a synchronous ovary.
    Description: Published
    Keywords: Schizothorax zarudnyi ; Reproduction ; Species ; Temperature ; Spawning ; GSI ; Oocyte ; Ovary
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.41-56
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  • 7
    Publication Date: 2021-05-19
    Description: Determination of the number of ovum micropyle in three sturgeon species had been the main goal of this research. The study was conducted on 44 specimens of female Persian sturgeon (Acipenser Persicus), 13 specimens of female Stellate sturgeon (A.stellatus) and 8 specimens of female beluga (Huso huso). 50 ova were randomly collected and evaluated from each specimen and totally the micropyles of 3250 ova from three sturgeon species were studied. From the 44 specimens of Persian sturgeon, 13 belonging to the South-East area of Caspian Sea; Golestan province, Shahid Marjani center and 31 of them from the South-West area of Caspian Sea; Guilan province, Shahid Beheshti center; including 21 specimens from the sea and 10 ones from Sefid-rud River. The entire stellate sturgeon specimens and beluga specimens were collected from Guilan province and Golestan province respectively. Based on achieved from results, the average number of micropyls of Persian sturgeon, inhabitant of the eastern part of Caspian sea, was 7.7±1.59 for the western part was 8.7±1.75 and for Persian sturgeon catched from Sefid-rud was 8.3 ± 1.6 in each ovum. Based on evaluation and statistic comparison, no significant difference (P〉0.95) was observed between Persian sturgeon of the East and West part, in terms of micropyle number. The average number of micropyle of stellate sturgeon was 4.7±1.51 and for beluga was 5.19±1.59 in each ovum. The average diameters of outer membrane micropyle of ovum in A.Persicus, A.Stellaus and Huso huso were 21.97±1.05 micron, 21.5±1.2 micron and 22.1±0.7 micron respectively; and the average diameters of inner membrane micropyle of ovum were 18.82±1.1 micron, 17.4±1.6 micron and 18.6±1.7 micron respectively.
    Description: Published
    Keywords: Micropyle ; Ovum ; Sturgeon ; Species ; Specimens ; Acipenser Persicus ; A.stellatus ; beluga ; Huso huso ; Beluga ; A.Persicus
    Repository Name: AquaDocs
    Type: Journal Contribution , Not Known
    Format: pp.35-48
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  • 8
    Publication Date: 2021-05-19
    Description: We studied genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii using Random Amplified Polymorphic DNA (RAPD) technique. The DNA of tail fin tissue of three A. persicus and A. gueldenstaedtii were extracted using phenol-chloroform method. After electrophoresis of the samples by agarose gel, their concentrations were regulated and Polymerase Chain Reaction (PCR) was conducted by 53 primers. PCR products were electrophoresed on polyacrylamide gel and silver staining was done to reveal the DNA bands of the samples. Among 53 primers, 17 had no site on genomic DNA of A. persicus and A. gueldenstaedtii and did not produce any bands while the remaining 36 primers showed band pattern. Analyzing the PCR products data using RAPD PLOT program showed that the maximum and minimum genetic distance between species were 73% and 65% respectively. Also, the mean difference between the species was 70% and the maximum and minimum genetic resemblance between the two species were 35% and 27% respectively. Based on the results, we conclude that A. persicus is an independent species from A. gueldenstaedtii.
    Description: Published
    Keywords: Acipenser persicus ; Acipenser gueldenstaedtii ; RAPD technique ; Genetic ; Tissue ; Polymerase Chain Reaction ; Polyacrylamide ; A. persicus ; A. gueldenstaedtii ; DNA ; Species
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.91-102
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  • 9
    Publication Date: 2021-05-19
    Description: Genetic variation of ship sturgeon (Acipenser nudiventris) from the Caspian Sea was investigated using NADH5/6 gene and PCR-RFLP analysis. A total of 80 specimens of the fish were collected from the south Caspian Sea and the Ural River from Kazakhstan. mtDNA ND5/6 gene was amplified by polymerase chain reaction (PCR) digested using 39 Endonucleases Restriction Enzyme. Of the 39 enzymes, five showed polymorphism. Totally, ten composite haplotypes among 80 specimens were detected. Haplotype AAAAA showed maximum frequency (57.5%) whereas haplotypes BBAAA and BABAA showed minimum frequency (1.2%). Haplotype AAAAB was recognized specifically in Ural River specimens. Average haplotype and nucleotide diversity was 0.8516 and 0.007 respectively. Compared to other sturgeon species living in the Caspian Sea, nucleotide diversity of Ship Sturgeon was much lower (0.007). This may be due to smaller population size of this species. Monte-Carol simulation using 1000 interaction did not show any significant differences between haplotype distribution of the fish sampled in the south Caspian Sea (X2=35.48 , P=0.74). However, we detected a significant difference between haplotype of Ship Sturgeon from Ural River and the south Caspian Sea. We conclude that Ship Sturgeon from Ural River is different from the fish in the south Caspian Sea and suggest Cfr131 enzyme as a molecular marker for population differentiation in the Caspian Sea.
    Description: Published
    Keywords: Ship sturgeon ; Acipenser nudiventris ; Ural River ; PCR-RFLP ; ND5/6 gene ; Enzyme ; Diversity ; Population ; Species ; Genetic ; Specimens
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.151-164
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  • 10
    Publication Date: 2021-05-19
    Description: Sturgeon are considered one of the most valuable fishes of the world that migrate to the rivers leading to the Caspian Sea, including the Sefirud River, to spawn. In the post this river was known as the reservoir for millions of sturgeon larvae, but at present due to illegal fishing in the river and the construction of the Tarik Dam, Manjil Dam and the Sangar Dam in the migratory route of these fishes and so also the decrease in the water flow of this river, it is losing its popularity. In order to study the natural reproduction of sturgeon in the river, an attempt to find larvae of different species of sturgeon was made from 19 May, 1996 to 13 June 1996. Six sampling stations were located from the beginning of the Sangar Dam as far as the sea shore (about 40 Km) for this purpose. Different methods of catch were employed including beach seine nets (mesh size 1, 4 and 8mm). “CALO” a local fishing effort (mesh size 4mm) and conical nets (mesh size 1 and 4 mm). On the whole out of 268 times that nets were cast, 1853200 Km of the study area was survey and totally 514500 fishes belonging to the fallowing species were caught. Liza auratus, Chalcalburnus chalcoides, Varicorhinus capoeta, Cobitis sp., Esox lucius, Silurus glanis, Abramis baranta, Carassius auratus, Cyprinus carpio, Tinca tinca, Stizostedion lucioperca , Alosa caspia , Syngnathus nigrolineatus, Barbus sp. , Anthemia boyeri, Pungitius platygaster and Gobius sp. No larvae of sturgeons were observed during this study. Although 218 brood fishes were caught in the Sefirud River in 1996, due to fishing pressure, illegal fishing, deterioration of spawning grounds and restrictions in the migratory route resulting from the construction of dams, no signs of natural reproduction were observed in the Sefirud River. Therefore it can be concluded that even if natural reproduction did take place in the Sefidrud River, the incidences were very low.
    Description: Published
    Keywords: Sturgeon ; Larvae ; Species ; Conical nets ; Survey ; Liza auratus ; Chalcalburnus chalcoides ; Varicorhinus capoeta ; Cobitis sp . ; Esox lucius ; Silurus glanis ; Abramis baranta ; Carassius auratus ; Cyprinus carpio ; Tinca tinca ; Stizostedion lucioperca ; Alosa caspia ; Syngnathus nigrolineatus ; Barbus sp. ; Anthemia boyeri ; Pungitius platygaster ; Gobius sp. ; Spawning ; Migratory
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.69-82
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