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  • RFLP  (126)
  • Wheat  (89)
  • phosphorus  (83)
  • Springer  (294)
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  • 1
    Digitale Medien
    Digitale Medien
    Chromosomal rearrangements in the rye genome relative to that of wheat (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 673-680 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Translocations ; Rye ; RFLP ; Genetic maps ; Comparative mapping ; Co-linearity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An RFLP-based genetic map of Secale Cereale has provided evidence for multiple evolutionary translocations in the rye genome relative to that of hexaploid wheat. DNA clones which have previously been mapped in wheat indicated that chromosome arms 2RS, 3RL, 4RL, 5RL, 6RS, 6RL, 7RS and 7RL have all been involved in at least one translocation. A possible evolutionary pathway, which accounts for the present day R genome relative to the A, B and D genomes of wheat, is presented. The relevance of these results for strategies designed to transfer useful genes from rye, and probably other related species, to wheat is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225004
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  • 2
    Digitale Medien
    Digitale Medien
    RFLP mapping of genes affecting plant height and growth habit in rye (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 1049-1054 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Rye ; Dwarfism ; Vernalisation ; Genetic mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary RFLP mapping of chromosome 5R in the F3 generation of a rye (Secale cereale L.) cross segregating for gibberellic acid (GA3)-insensitive dwarfness (Ct2/ct2) and spring growth habit (Sp1/sp1) identified RFLP loci close to each of these agronomically important genes. The level of RFLP in the segregating population was high, and thus allowed more than half of the RFLP loci to be mapped, despite partial homozygosity in the parental F2 plant. Eight further loci were mapped in an unrelated F2 rye population, and a further two were placed by inference from equivalent genetic maps of related wheat chromosomes, allowing a consensus map of rye chromosome 5R, consisting of 29 points and spanning 129 cM, to be constructed. The location of the ct2 dwarfing gene was shown to be separated from the segment of the primitive 4RL translocated to 5RL, and thus the gene is probably genetically unrelated to the major GA-insensitive Rht genes of wheat located on chromosome arms 4BS and 4DS. The map position of Sp1 is consistent both with those of wheat Vrn1 and Vrn3, present on chromosome arms 5AL and 5DL, respectively, and with barley Sh2 which is distally located on chromosome arm 7L (= 5HL).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215046
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  • 3
    Digitale Medien
    Digitale Medien
    A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 705-712 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Mapping ; Barley ; Genome ; Centromeres
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222660
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  • 4
    Digitale Medien
    Digitale Medien
    Genomic mapping of defense response genes in wheat (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 226-233 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Molecular mapping ; Wheat ; Resistance ; Defense response genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Defense response (DR) genes are a broad class involved in plant defense. In this study we mapped 36 probes representing seven classes of defense response genes. This collection of probes represents genes involved in the hypersensitive response (HR), pathogenesis-related (PR) genes, genes for the flavonoid metabolic pathway, genes encoding proline/glycine-rich proteins, ion channel regulators, lipoxygenase, lectin, and others. Using nullisomic-tetrasomic lines of ‘Chinese Spring’, we were able to assign at least 167 loci to the 21 chromosomes of wheat. Homoeologous group 7 chromosomes possessed the most DR loci followed by group 2. Sixty-two loci were placed on existing genetic linkage maps of wheat. Map locations indicated that the DR gene loci are not randomly distributed throughout the wheat genome, but rather are located in clusters and/or in distal gene-rich regions of the chromosomes. Knowledge of the chromosomal locations and genome organization of DR genes will be useful for candidate gene analysis of quantitative trait loci.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051062
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  • 5
    Digitale Medien
    Digitale Medien
    Early post-pollination events in hexaploid wheat × maize crosses (1990)
    Springer
    Sexual plant reproduction 3 (1990), S. 70-76 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Wheat ; Maize ; Hybridization ; Fertilization ; Karyogamy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Cytological events in the first 12 h after pollination were studied in crosses between the hexaploid wheat genotype Chinese Spring and the maize genotype Seneca 60. A pollen tube was first observed in the embryo sac 4 h after pollination, and maize sperm nuclei were first observed in the embryo sac after 5 h. On 29 occasions two, and on 1 occasion three, pollen tubes penetrated the embryo sac. Four categories of aberration limiting the frequency of fertilization were identified: (1) in 20% of florets no pollen tube reached the embryo sac; (2) in at least 1.9% the pollen tube severely damaged the wheat egg cell and polar nuclei; (3) in 33% the maize sperm nuclei were not released from the pollen tube; and (4) in 16% the sperm nuclei were released into the embryo sac but failed to move to either of the wheat gametes. In the remaining 29% sperm nuclei were more often found in the egg cell than at the polar nuclei. The results suggest that karyogamy occurs with very high efficiency when a sperm nucleus reaches the egg cell, but with only about 50% efficiency when a sperm nucleus reaches the polar nuclei.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00189954
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  • 6
    Digitale Medien
    Digitale Medien
    The production of haploid wheat plants from wheat x maize crosses (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 393-397 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Maize ; Wide-crosses ; Embryo culture ; Haploids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Hybrid embryos from hexaploid wheat x maize crosses rapidly lose the maize chromosomes to produce haploid wheat embryos. Such embryos almost always aborted when left to develop on the plant, and only 1 was recovered from 2440 florets (0.17% of the expected number). Embryos had greater viability in spikelet culture, 47 (26.5% of the expected number) being recovered from 706 ovaries. Thirty-two of these embryos germinated to give green plants, 31 of which were haploid (21 wheat chromosomes) and 1 of which was euploid (42 wheat chromosomes). Spikelet culture enabled 17.1% of the expected number of embryos to be recovered as haploid plants, a 100-fold improvement on allowing embryos to develop in vivo. Ten haploid plants of ‘Chinese Spring’ (kr1, kr2), 13 plants of ‘Chinese Spring (Hope 5A)’ (kr1, Kr2), and 8 of ‘Hope’ (Kr1, Kr2) were recovered. The potential of wheat x maize crosses for wheat haploid production and for gene transfer from maize to wheat is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00265339
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  • 7
    Digitale Medien
    Digitale Medien
    Genetic characterisation of a further homoeoallelic series of grain esterase loci,Est-6, in wheat (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 294-296 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Grain esterase ; Wheat ; Isoelectric focussing ; Isozymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Isoelectric focussing in alkaline pH gels has permitted the identification of a new homoeoallelic series of genes,Est-6, encoding grain esterases in bread wheat,Triticum aestivum. Nullisomic analysis located these genes to the short arms of the homoeologous group 2 chromosomes. A search for polymorphism withinEst-6 revealed null alleles at each ofEst-A6,Est-B6 andEst-D6. A further homoeolocus,Est-M6, is present on chromosome arm2MS ofAegilops comosa.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01186070
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  • 8
    Digitale Medien
    Digitale Medien
    RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 174-178 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Rye ; Genetic mapping ; RFLP ; Storage protein ; Isozyme
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A map of chromosome 1R of rye was constructed using 16 molecular and biochemical loci. From long arm to short arm, known-function loci were placed in the order: XAdh — XLee — Glu-R1[Sec-3] — XPpdk-1R — XEm-1R-1 — XEm-1R-2 — Centromere — XNor-R1 —Gpi-R1 — XGli-R1 [Sec-1a] along with six anonymous genomic and cDNA clones from wheat. The map, which spans 106 cM with 12 loci clustered in a 15-cM region around the centromere, shows reasonably good agreement with previously published maps for the centromeric region, whereas the XNor-R1 — Gpi-R1 region gives a much larger distance than previously reported.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226209
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  • 9
    Digitale Medien
    Digitale Medien
    RFLP maps of potato and their alignment with the homoeologous tomato genome (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 49-57 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Potato ; Tomato ; Genetic maps
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An RFLP linkage map of the potato is presented which comprises 304 loci derived from 230 DNA probes and one morphological marker (tuber skin color). The self-incompatibility locus of potato was mapped to chromosome I, which is homoeologous to tomato chromosome I. By mapping chromosome-specific tomato RFLP markers in potato and, vice versa, potato markers in tomato, the different potato and tomato RFLP maps were aligned to each other and the similarity of the potato and tomato genome was confirmed. The numbers given to the 12 potato chromosomes are now in accordance with the established tomato nomenclature. Comparisons between potato RFLP maps derived from different genetic backgrounds revealed conservation of marker order but differences in chromosome and total map length. In particular, significant reduction of map length was observed in interspecific compared to intraspecific crosses. The distribution of regions with distorted segregation ratios in the genome was analyzed for four potato parents. The most prominent distortion of recombination was found to be caused by the self-incompatibility locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00229225
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  • 10
    Digitale Medien
    Digitale Medien
    Mapping genes for resistance to barley stripe rust (Puccinia striiformis f. sp. hordei) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 215-219 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Barley stripe rust ; RFLP ; QTL mapping ; Molecular marker-assisted backcrossing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two genes conferring resistance to the barley stripe rust found in Mexico and South America, previously identified as race 24, were mapped to the M arms of barley chromosomes 7 and 4 in a doubled haploid population using molecular markers and the quantitative trait loci (QTL) mapping approach. The resistance gene on chromosome 7 had a major effect, accounting for 57% of the variation in disease severity. The resistance gene on chromosome 4 had a minor effect, accounting for 10% of the variation in trait expression. Two pairs of restriction fragment length polymorphism markers are being used to introgress the resistance genes to North American spring barley using molecular marker-assisted backcrossing.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225900
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