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  • RFLP  (55)
  • Springer  (55)
  • 2005-2009
  • 1990-1994  (55)
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  • Springer  (55)
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  • 1
    Digitale Medien
    Digitale Medien
    Chromosomal rearrangements in the rye genome relative to that of wheat (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 673-680 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Translocations ; Rye ; RFLP ; Genetic maps ; Comparative mapping ; Co-linearity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An RFLP-based genetic map of Secale Cereale has provided evidence for multiple evolutionary translocations in the rye genome relative to that of hexaploid wheat. DNA clones which have previously been mapped in wheat indicated that chromosome arms 2RS, 3RL, 4RL, 5RL, 6RS, 6RL, 7RS and 7RL have all been involved in at least one translocation. A possible evolutionary pathway, which accounts for the present day R genome relative to the A, B and D genomes of wheat, is presented. The relevance of these results for strategies designed to transfer useful genes from rye, and probably other related species, to wheat is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225004
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  • 2
    Digitale Medien
    Digitale Medien
    RFLP mapping of genes affecting plant height and growth habit in rye (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 1049-1054 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Rye ; Dwarfism ; Vernalisation ; Genetic mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary RFLP mapping of chromosome 5R in the F3 generation of a rye (Secale cereale L.) cross segregating for gibberellic acid (GA3)-insensitive dwarfness (Ct2/ct2) and spring growth habit (Sp1/sp1) identified RFLP loci close to each of these agronomically important genes. The level of RFLP in the segregating population was high, and thus allowed more than half of the RFLP loci to be mapped, despite partial homozygosity in the parental F2 plant. Eight further loci were mapped in an unrelated F2 rye population, and a further two were placed by inference from equivalent genetic maps of related wheat chromosomes, allowing a consensus map of rye chromosome 5R, consisting of 29 points and spanning 129 cM, to be constructed. The location of the ct2 dwarfing gene was shown to be separated from the segment of the primitive 4RL translocated to 5RL, and thus the gene is probably genetically unrelated to the major GA-insensitive Rht genes of wheat located on chromosome arms 4BS and 4DS. The map position of Sp1 is consistent both with those of wheat Vrn1 and Vrn3, present on chromosome arms 5AL and 5DL, respectively, and with barley Sh2 which is distally located on chromosome arm 7L (= 5HL).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215046
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  • 3
    Digitale Medien
    Digitale Medien
    A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 705-712 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Mapping ; Barley ; Genome ; Centromeres
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222660
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  • 4
    Digitale Medien
    Digitale Medien
    RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 174-178 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Rye ; Genetic mapping ; RFLP ; Storage protein ; Isozyme
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A map of chromosome 1R of rye was constructed using 16 molecular and biochemical loci. From long arm to short arm, known-function loci were placed in the order: XAdh — XLee — Glu-R1[Sec-3] — XPpdk-1R — XEm-1R-1 — XEm-1R-2 — Centromere — XNor-R1 —Gpi-R1 — XGli-R1 [Sec-1a] along with six anonymous genomic and cDNA clones from wheat. The map, which spans 106 cM with 12 loci clustered in a 15-cM region around the centromere, shows reasonably good agreement with previously published maps for the centromeric region, whereas the XNor-R1 — Gpi-R1 region gives a much larger distance than previously reported.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226209
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  • 5
    Digitale Medien
    Digitale Medien
    RFLP maps of potato and their alignment with the homoeologous tomato genome (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 49-57 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Potato ; Tomato ; Genetic maps
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An RFLP linkage map of the potato is presented which comprises 304 loci derived from 230 DNA probes and one morphological marker (tuber skin color). The self-incompatibility locus of potato was mapped to chromosome I, which is homoeologous to tomato chromosome I. By mapping chromosome-specific tomato RFLP markers in potato and, vice versa, potato markers in tomato, the different potato and tomato RFLP maps were aligned to each other and the similarity of the potato and tomato genome was confirmed. The numbers given to the 12 potato chromosomes are now in accordance with the established tomato nomenclature. Comparisons between potato RFLP maps derived from different genetic backgrounds revealed conservation of marker order but differences in chromosome and total map length. In particular, significant reduction of map length was observed in interspecific compared to intraspecific crosses. The distribution of regions with distorted segregation ratios in the genome was analyzed for four potato parents. The most prominent distortion of recombination was found to be caused by the self-incompatibility locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00229225
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  • 6
    Digitale Medien
    Digitale Medien
    Mapping genes for resistance to barley stripe rust (Puccinia striiformis f. sp. hordei) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 215-219 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Barley stripe rust ; RFLP ; QTL mapping ; Molecular marker-assisted backcrossing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two genes conferring resistance to the barley stripe rust found in Mexico and South America, previously identified as race 24, were mapped to the M arms of barley chromosomes 7 and 4 in a doubled haploid population using molecular markers and the quantitative trait loci (QTL) mapping approach. The resistance gene on chromosome 7 had a major effect, accounting for 57% of the variation in disease severity. The resistance gene on chromosome 4 had a minor effect, accounting for 10% of the variation in trait expression. Two pairs of restriction fragment length polymorphism markers are being used to introgress the resistance genes to North American spring barley using molecular marker-assisted backcrossing.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225900
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  • 7
    Digitale Medien
    Digitale Medien
    Comparative RFLP-based genetic maps of barley chromosome 5 (1H) and rye chromosome 1R (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 339-344 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Barley ; Genetic mapping ; RFLP ; Storage proteins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A genetic map of barley chromosome 5 (1H) was constructed using DNA markers. Seventeen loci were mapped to 15 locations, and these included the known-function loci (in order from the most distal on the long arm) XAdh (alcohol dehydrogenase), XLec (homologous to wheat germ agglutinin), XHor3 (D-hordein), XPpdk (pyruvate orthophosphate dikinase), centromere, XIcal (chymotrypsin inhibitor), and 6 loci in the B- and C-hordein cluster towards the end of the short arm. The gene order on the barley map agreed closely with that of chromosome 1 of rye. Intervarietal comparisons showed that single-copy cDNA and genomic DNA probes revealed about twice the level of RFLPs found in wheat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00229492
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  • 8
    Digitale Medien
    Digitale Medien
    Analyses of Phaseolus vulgaris L. and P. coccineus Lam. hybrids by RFLP: preferential transmission of P. vulgaris alleles (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 703-709 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Phaseolus interspecific hybrid ; RFLP ; Beans
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Restriction fragment length polymorphism (RFLP) was determined among P. vulgaris genotypes and Phaseolus species using 19 probes. The incidence of polymorphism was high (70–86%) between species, but relatively low (22–26%) between genotypes of P. vulgaris. Suitable probes were identified for the analysis of P. vulgaris and P. coccineus hybrids. The segregation pattern in F2 populations was Mendelian for two probes (LHB and VEE20) and non-Mendelian for GS-g, CHS, and CHI. Statistical analyses indicated gametic selection with preferential transmission of the P. vulgaris alleles, which may account for the selective recovery of P. vulgaris progeny types observed earlier. The available hybrids of P. vulgaris and P. coccineus and the high degree of interspecific RFLP will facilitate the construction of a linkage map for Phaseolus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226740
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  • 9
    Digitale Medien
    Digitale Medien
    Nonhomoeologous translocations between group 4, 5 and 7 chromosomes within wheat and rye (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 305-312 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Rye ; RFLP ; Isozymes ; Evolutionary translocations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genetic maps of wheat chromosome 4A and rye chromosome arm 5RL, and the chromosomal locations of 70 sets of isozyme and molecular homoeoloci have been used to further define the structure of wheat chromosomes 4A, 5A and 7B, and rye chromosomes 4R, 5R and 7R. We provide evidence, for the first time, which is consistent with the presence of an interstitial segment on 4AL originating from 5AL, and of a segment originally from 5RL on 7RS. The evolutionary origins of the present chromosomes are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224276
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  • 10
    Digitale Medien
    Digitale Medien
    Molecular taxonomic relationships in the genus Vigna based on RFLP analysis (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 97-104 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Vigna ; Numerical taxonomy ; RFLP ; Asiatic grams ; Cowpea ; Bambara groundnut
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The taxonomy of the genus Vigna has been primarily based on morphological attributes. We have used 27 genomic clones from soybean, common bean, mungbean and cowpea to examine restriction fragment length polymorphism (RFLP) among 44 accessions of different species belonging to four subgenera of the genus Vigna. One accession each of common bean (Phaseolus vulgaris) and soybean (Glycine max) was included in the study. Total DNA from the various genotypes was digested with one restriction enzyme (Eco RV). Results of a numerical taxonomic analysis showed a high level of genetic variation within the genus with a remarkably higher amount of variation associated with Vigna sp. from Africa relative to those from Asia. The distinctness of the Asiatic grams in subgenus Ceratotropis, cowpea in section Catiang, bambara groundnut (V. subterranean) and members of the subgenus Plectotropis was elucidated by this study. Members of the subgenus Plectotropis were closer in genome homology to those of subgenus Vigna section Catiang than to those of subgenus Ceratotropis. The relative positions of some genotypes to one another on the dendrogram and minimum spanning tree were discussed in regard to hybridisations aimed generating well-saturated genomic maps and interspecies transfer of desirable genes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223813
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