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  • 1
    Electronic Resource
    Electronic Resource
    Monitoring the rate of oxygen consumption in plant cell suspensions (1997)
    Springer
    Plant cell, tissue and organ culture 51 (1997), S. 111-117 
    Details
    ISSN: 1573-5044
    Keywords: Nicotiana tabacum ; oxidative burst ; oxygen electrode ; Pseudomonas solanacearum ; Pseudomonas syringae ; respiratory burst ; Solanumtuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described that allows the rate of oxygen consumption to be monitored in plant cell suspensions. The method utilized oxygen electrodes placed in beakers of plant cells subjected to various treatments. The voltage readings from calibrated electrodes were converted to % oxygen (100% equals air equilibration) and the rate of oxygen consumption was estimated by calibration graphs made with no cells present. This system simultaneously monitors one to sixteen or more samples, allowing comparison of treatments on identically treated cells. We have used this method to study the respiratory burst of plant cells produced in response to viable or heat-killed bacteria. Because the system was computer-monitored and open to the atmosphere, data could be collected over several hours. Various factors that affected the measurement of dissolved oxygen concentration with this technique were explored and considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005997126293
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  • 2
    Electronic Resource
    Electronic Resource
    An improved method for monitoring cell death in cell suspension and leaf disc assays using evans blue (1994)
    Springer
    Plant cell, tissue and organ culture 39 (1994), S. 7-12 
    Details
    ISSN: 1573-5044
    Keywords: cell viability ; hypersensitive response ; Pseudomonas syringae ; soybean ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell viability or cell death is an important variable to monitor in many studies of host/pathogen interactions. However for studies that focus on events within the first few hours of the interaction, many of the viability assays currently being used are either too laborious and time consuming or measure the cell's temporary metabolic state rather than irreversible cell death. Evans blue has proven over the years to be a dependable stain for microscopic determination of cell death. We have used this stain to develop a spectrophotometric procedure that allows rapid, reproducible quantification of the stain retained by dead cells. This spectrophotometric procedure was used to compare plant/bacteria interactions involving either soybean/Pseudomonas syringae pv. glycinea or tobacco/P. syringae pv. syringae. Relative increases in cell death during these interactions in suspension cell systems were measured by both the spectrophotometric and microscopic technique and found to be similar. The spectrophotometric procedure was also adapted for leaf disc assays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037585
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  • 3
    Electronic Resource
    Electronic Resource
    Bubble formation at vibrated orifices: Medium-chamber-volume region (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 37-44 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The bubble formation process at small, single, circular orifices at low gas flow rates is modified when the system is vertically vibrated in a sinusoidal fashion.For systems with significant gas chamber volume, bubble volume in the vibrated case is smaller than in the nonvibrated case. The vibrations increase the amount of liquid weeping through the orifice into the gas chamber. A simple inviscid model adequately predicts bubble formation in the medium-chamber-volume region at low values of amplitude and frequency of vibration and low viscosities. The boundaries of the transition from the FR ≫ 1 to the FR = 1 regions are described in terms of “the acceleration number/Eötvös number”.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690300107
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  • 4
    Electronic Resource
    Electronic Resource
    Determination of Molecular Weight of High Polymers by Measurement of Osmotic Pressures at Low Concentrations (1951)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 6 (1951), S. 585-600 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: An equilibrium osmometer for the measurement of very low osmotic pressures has been designed. It is constructed of metal and employs a large horizontal membrane. A rapid approach to equilibrium is obtained by the use of a fine capillary and a bacterial cellulose membrane (Masson and Melville) in conjunction with complete thermostatting. To avoid displacement, the membrane is stretched in a frame and held over a grooved domed support of the Carter-Record type. Details of procedure, including concentration estimation and the preparation of membranes, are given.Measurements of the osmotic pressure of a fractionated sample of polystyrene, of molecular weight approximately one million, have served to determine the precision of the osmometer. At a pressure of 0.026 cm. of chloroform, the standard deviation was found to be 0.002 cm. The importance of correcting for the density of the solution, when measuring high molecular weights, is discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1951.120060508
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  • 5
    Electronic Resource
    Electronic Resource
    VII. Umlagerung der βγ-Hexensäure (Hydrosorbinsäure) (1894)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 283 (1894), S. 117-128 
    Details
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.18942830110
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  • 6
    Electronic Resource
    Electronic Resource
    Polymerization of vinyl monomers stabilized withp-methoxyphenol (1960)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 47 (1960), S. 498-498 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1960.1204714952
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  • 7
    Electronic Resource
    Electronic Resource
    An apple II based datasystem for AES depth profiling on a varian spectrometer (1985)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 7 (1985), S. 150-154 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A description is given of an Apple II based Auger spectroscopy datasystem specifically designed for depth profiling applications. Software features which are not available on commercial systems are emphasized. In particular, the utility of peak overlap and noise threshold subtraction routines are demonstrated with reference to two examples of practical profiling.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740070307
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  • 8
    Electronic Resource
    Electronic Resource
    X-ray diffraciton studies of the melting of linear polyethyleneThis work was supported in part by a grant from the Army Research Office (Durham) and a contract with the Division of Biology, Atomic Energy Commission. (1965)
    New York : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Letters 3 (1965), S. 339-341 
    Details
    ISSN: 0449-2986
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1965.110030418
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