Publication Date:
2003-07-05
Description:
Escherichia coli entry into the bladder is met with potent innate defenses, including neutrophil influx and epithelial exfoliation. Bacterial subversion of innate responses involves invasion into bladder superficial cells. We discovered that the intracellular bacteria matured into biofilms, creating pod-like bulges on the bladder surface. Pods contained bacteria encased in a polysaccharide-rich matrix surrounded by a protective shell of uroplakin. Within the biofilm, bacterial structures interacted extensively with the surrounding matrix, and biofilm associated factors had regional variation in expression. The discovery of intracellular biofilm-like pods explains how bladder infections can persist in the face of robust host defenses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Anderson, Gregory G -- Palermo, Joseph J -- Schilling, Joel D -- Roth, Robyn -- Heuser, John -- Hultgren, Scott J -- AI29549/AI/NIAID NIH HHS/ -- AI48689/AI/NIAID NIH HHS/ -- DK51406/DK/NIDDK NIH HHS/ -- DK64540/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2003 Jul 4;301(5629):105-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12843396" target="_blank"〉PubMed〈/a〉
Keywords:
*Adhesins, Bacterial
;
Adhesins, Escherichia coli
;
Animals
;
*Antigens, Bacterial
;
Bacterial Outer Membrane Proteins/analysis
;
*Biofilms
;
Colony Count, Microbial
;
Epithelial Cells/microbiology/ultrastructure
;
Escherichia coli/growth & development/immunology/*pathogenicity/ultrastructure
;
Escherichia coli Infections/immunology/*microbiology/pathology
;
*Escherichia coli Proteins
;
Female
;
Fimbriae, Bacterial/physiology/ultrastructure
;
Freeze Fracturing
;
Immunity, Innate
;
Membrane Glycoproteins/analysis
;
Mice
;
Mice, Inbred C3H
;
Microscopy, Electron
;
Microscopy, Electron, Scanning
;
Polysaccharides, Bacterial/analysis
;
Urinary Bladder/immunology/*microbiology/ultrastructure
;
Urinary Bladder Diseases/immunology/*microbiology/pathology
;
Urinary Tract Infections/immunology/*microbiology/pathology
;
Urothelium/microbiology/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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