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  • 1
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    Proximate and fatty acid composition of liver and fatty tissue of patincatfish (Pangasianodon hypophthalmus) (2018)
    Details
    Publication Date: 2021-05-19
    Description: The visceral storage fat and liver of patin catfish (Pangasianodon hypophthalmus) are normally discarded, which incurs cost and can cause environmental pollution. However, these may be potential sources to extract fish oil. The proximate and fatty acid compositions of liver and fatty tissue of patin catfish were investigated to evaluate the suitability of these by-products for extracting fish oil. Fat was extracted using a low temperature solvent extraction method. The average fat content of fatty tissue and liver of females were 77.64 and 11.71%, respectively, whereas in males this was73.23 and 9.59%, respectively. Fatty acids found in the extracted oil of these byproducts were C12:0, C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C18:4, C20:0, C20:1, C20:4, C20:5, and C22:6.The major fatty acids presented in these tissues were palmitic (C16:0), oleic (C18:1n-9), and linoleic acid (C18:2 n-6). The total amount of polyunsaturated fatty acids of liver from male and female patin catfish were 13.31 and 13.30%, respectively, whereas in the fatty tissue these were11.64 and 12.09%, respectively. The n-3 to n-6 ratios of liver and fatty tissue of females were 1.61and 0.95, respectively, whereas in male fish these were 1.31 and 1.05, respectively. Results of this study indicated that the liver and fatty tissues of patin catfish are suitable sources of fish oil specifically due to the presence of monounsaturated and n-3 polyunsaturated fatty acids.
    Description: Published
    Keywords: Biology ; Physiology ; Pangasianodon hypophthalmus ; Catfish ; Fatty acid ; Liver ; Fatty tissue ; Tissues ; Liver
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.541-549
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  • 2
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    A simple and rapid method for blood collection from walking catfish, Clarias batrachus (Linneaus, 1758) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance of the caudal vein. Unfortunately, all these procedures are practically found to be slow and stressful to Clarias batrachus, including the popular caudal vein approach, likely due to the small size of caudal veins relative to the size of the species. In line with the universal ethical recommendations for taking blood from small research animals, we propose an alternative one-operator approach for C. batrachus that is simple, rapid and without the need to sacrifice the fish as with other methods. This procedure targets the dorsal aorta (a relatively larger blood vessel) in a sedated fish, punctured by inserting a needle directly from the anterior part of the anal fin about 2-5 mm behind the genital papilla, to draw the desired amount of blood. The technique is a one-operator procedure not requiring the help of an assistant or any special equipment to restrain the fish. The operation of the protocol is unique since it permits the continuous collection of blood from the same experimental fish over a varied time course and reduces the need for a large number of replicate animals. The advantages of the proposed protocol are also highlighted and discussed in detail.
    Description: Published
    Keywords: Haematology ; Catfish ; Aquaculture ; Blood sampling ; Clarias batrachus
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.935-944
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  • 3
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    A simple and rapid method for blood collection from walking catfish, Clarias batrachus (Linneaus, 1758) (2021)
    In:  http://aquaticcommons.org/id/eprint/23130 | 18721 | 2018-06-18 17:16:30 | 23130 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-13
    Description: Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance of the caudal vein. Unfortunately, all these procedures are practically found to be slow and stressful to Clarias batrachus, including the popular caudal vein approach, likely due to the small size of caudal veins relative to the size of the species. In line with the universal ethical recommendations for taking blood from small research animals, we propose an alternative one-operator approach for C. batrachus that is simple, rapid and without the need to sacrifice the fish as with other methods. This procedure targets the dorsal aorta (a relatively larger blood vessel) in a sedated fish, punctured by inserting a needle directly from the anterior part of the anal fin about 2-5 mm behind the genital papilla, to draw the desired amount of blood. The technique is a one-operator procedure not requiring the help of an assistant or any special equipment to restrain the fish. The operation of the protocol is unique since it permits the continuous collection of blood from the same experimental fish over a varied time course and reduces the need for a large number of replicate animals. The advantages of the proposed protocol are also highlighted and discussed in detail.
    Keywords: Biology ; Fisheries ; Haematology ; Catfish ; Aquaculture ; Blood sampling ; Clarias batrachus ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 935-944
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  • 4
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    Feeding characteristics of Neogobius caspius in the south west coastline of the Caspian Sea (Gilan Province) (2021)
    In:  http://aquaticcommons.org/id/eprint/22360 | 18721 | 2018-03-27 22:34:32 | 22360 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-04
    Description: Neogobius caspius is an endemic species of the Caspian Sea which plays an important role in food chain as a predatory fish. The main aim of this study was to investigate selected feeding characteristics of Neogobius caspius in the south west coasts of the Caspian Sea. Monthly sampling was carried out using a bottom trawler at three stations (Astara, Anzali and Chabuksar) in three depths (0-5, 5-10 and 10-15m) on a monthly basis from October 2004 to September 2005. Relative gut length (RLG) was less than one suggesting that this species is carnivorous. RLG was significantly lower in older fish but gut length was longer (P〈0.05). Intensity of fullness was below the favorite degree (IF〈400) from October to March, however, it was higher (400〈IF〈900) from April to October at all stations, indicating that feeding conditions for this species is suitable in the study area. Study showed that N. caspius mainly fed on molluscs (Food preference, FP=100%), worms (FP=89%) and crustaceans (FP=74%), hence, this species is considered as euryphagus species.
    Description: Article includes abstract in Farsi on last page.
    Keywords: Biology ; Ecology ; Fisheries ; Neogobius caspius ; Feeding characteristics ; Relative gut length ; Food preference ; Caspian Sea ; Gilan Province ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 127-140
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  • 5
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    Proximate and fatty acid composition of liver and fatty tissue of patin catfish (Pangasianodon hypophthalmus) (2021)
    In:  http://aquaticcommons.org/id/eprint/22707 | 18721 | 2018-05-16 19:00:07 | 22707 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-09
    Description: The visceral storage fat and liver of patin catfish (Pangasianodon hypophthalmus) are normally discarded, which incurs cost and can cause environmental pollution. However, these may be potential sources to extract fish oil. The proximate and fatty acid compositions of liver and fatty tissue of patin catfish were investigated to evaluate the suitability of these by-products for extracting fish oil. Fat was extracted using a low temperature solvent extraction method. The average fat content of fatty tissue and liver of females were 77.64 and 11.71%, respectively, whereas in males this was 73.23 and 9.59%, respectively. Fatty acids found in the extracted oil of these byproducts were C12:0, C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C18:4, C20:0, C20:1, C20:4, C20:5, and C22:6. The major fatty acids presented in these tissues were palmitic (C16:0), oleic (C18:1 n-9), and linoleic acid (C18:2 n-6). The total amount of polyunsaturated fatty acids of liver from male and female patin catfish were 13.31 and 13.30%, respectively, whereas in the fatty tissue these were 11.64 and 12.09%, respectively. The n-3 to n-6 ratios of liver and fatty tissue of females were 1.61 and 0.95, respectively, whereas in male fish these were 1.31 and 1.05, respectively. Results of this study indicated that the liver and fatty tissues of patin catfish are suitable sources of fish oil specifically due to the presence of monounsaturated and n-3 polyunsaturated fatty acids.
    Keywords: Biology ; Fisheries ; Catfish ; Fatty acid ; Liver ; Fatty tissue ; Biology ; physiology ; Malaysia ; Pangasianodon hypophthalmus ; patin catfish
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 541-549
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