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  • 1
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    Development of single-locus DNA microsatellite markers using 5′anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia (2011)
    Springer
    Details
    Publication Date: 2011-07-10
    Print ISSN: 0301-4851
    Electronic ISSN: 1573-4978
    Topics: Biology
    Published by Springer
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  • 2
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    Reproductive characteristics of Neogobius caspius from the southwest coasts of the Caspian Sea, Guilan Province of Iran (2018)
    Details
    Publication Date: 2021-05-19
    Description: Neogobius caspius is an endemic fish species in the Caspian Sea belonging to the family Gobiidae that ecologically play important role as a predator fish in the food chain .The main objective of this research was determination of some reproductive properties of N. caspius in the southwest coasts of the Caspian Sea. Sampling was carried out with a bottom trawler in three stations (Astara, Anzali and Chabuksar) from October 2004 until September 2005. The species was identified based on meristic parameters. The maximum gonadosomatic index (GSI) was recorded at 7.52% for male and 100.12% for female fish in June. A sharp decrease in GSI value from June to the end of September in both sexes was observed and we found that the spawning of the species takes place several times each year. Hence, the species is considered a late spawner. Female to male sex ratio was 1.47:1. Absolute fecundity was in the range 212-1234 eggs and the relative fecundity fluctuated between 18.73 and 29.89 eggs per each gram of the body weight. The correlation between absolute and relative fecundity with age was positive and with total length and full weight was negative. Egg diameter was in the range 0.06 to 0.23mm. The correlation between egg diameter, age and total length was positive.
    Description: Published
    Keywords: Food chains ; Endemic species ; Brackishwater fish ; Neogobius ; Gobiidae ; Neogobius caspius ; Brackish ; Biological surveys ; Sex ratio ; Males ; Gonadosomatic index ; Fecundity ; Fish physiology ; Reproductive cycle ; Body size ; Reproduction ; Females
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.87-98
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  • 3
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    Feeding characteristics of Neogobis caspius in the south west coastline of the Caspian Sea (Gilan Province) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Neogobius caspius is an endemic species of the Caspian Sea which plays an important role in food chain as a predatory fish. The main aim of this study was to investigate selected feeding characteristics of Neogobius caspius in the south west coasts of the Caspian Sea. Monthly sampling was carried out using a bottom trawler at three stations (Astara, Anzali and Chabuksar) in three depths (0-5, 5-10 and 10-15m) on a monthly basis from October 2004 to September 2005. Relative gut length (RLG) was less than one suggesting that this species is carnivorous. RLG was significantly lower in older fish but gut length was longer (P〈0.05). I ntensity of fullness was below the favorite degree (IF〈400) from October to March, however, it was higher (400N. caspius mainly fed on molluscs (Food preference, FP=100%), worms (FP=89%) and crustaceans (FP=74%), hence, this species is considered as euryphagus species.
    Description: Published
    Keywords: Caspius ; Feeding characteristics ; Relative gut length ; Food preference
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.127-140
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  • 4
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    Proximate and fatty acid composition of liver and fatty tissue of patincatfish (Pangasianodon hypophthalmus) (2018)
    Details
    Publication Date: 2021-05-19
    Description: The visceral storage fat and liver of patin catfish (Pangasianodon hypophthalmus) are normally discarded, which incurs cost and can cause environmental pollution. However, these may be potential sources to extract fish oil. The proximate and fatty acid compositions of liver and fatty tissue of patin catfish were investigated to evaluate the suitability of these by-products for extracting fish oil. Fat was extracted using a low temperature solvent extraction method. The average fat content of fatty tissue and liver of females were 77.64 and 11.71%, respectively, whereas in males this was73.23 and 9.59%, respectively. Fatty acids found in the extracted oil of these byproducts were C12:0, C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C18:4, C20:0, C20:1, C20:4, C20:5, and C22:6.The major fatty acids presented in these tissues were palmitic (C16:0), oleic (C18:1n-9), and linoleic acid (C18:2 n-6). The total amount of polyunsaturated fatty acids of liver from male and female patin catfish were 13.31 and 13.30%, respectively, whereas in the fatty tissue these were11.64 and 12.09%, respectively. The n-3 to n-6 ratios of liver and fatty tissue of females were 1.61and 0.95, respectively, whereas in male fish these were 1.31 and 1.05, respectively. Results of this study indicated that the liver and fatty tissues of patin catfish are suitable sources of fish oil specifically due to the presence of monounsaturated and n-3 polyunsaturated fatty acids.
    Description: Published
    Keywords: Biology ; Physiology ; Pangasianodon hypophthalmus ; Catfish ; Fatty acid ; Liver ; Fatty tissue ; Tissues ; Liver
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.541-549
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  • 5
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    Effect of using various amounts of patin (Pangasianodon hypophthalmus) fish oil on physical and chemical properties of moisturizing hand cream (2019)
    Details
    Publication Date: 2021-05-19
    Description: Hand cream is used for rehydrating and improving skin properties. The skin is the first point of contact with the environment. Thus having healthy skin with proper barrier properties is quite important. The beneficial effects of fish oil on skin make it a favorable ingredient to be used in hand care products. In the present study, the efficacy and stability of various hand cream formulations using 0, 1.0, 2.5 and 5.0% of fish oil were investigated and compared. The appearance, odor, texture, and pH of formulations containing 1 and 2.5% of fish oil remained stable in very good qualities during the storage, whereas the related values to the appearance and texture of formulation containing 5.0% of fish oil at the end of 6 months of storage at 45°C were significantly (p〈0.05) lower than other formulations and previous evaluations of this formulation. Weight loss was not observed in all formulations under study.
    Description: Published
    Keywords: Pangasianodon hypophthalmus ; Physical ; Chemical ; Stability ; Cosmetic ; Formulation ; Hand cream ; Fish oil
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.215-223
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  • 6
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    Effects of stocking density on growth performance and survival of three male morphotypes in all-male culture of Macrobrachium rosenbergii (De Man) (2018)
    Details
    Publication Date: 2021-05-19
    Description: A study was conducted to appraise the effect of different stocking densities on three male morphotypes named blue claw (BC), orange claw (OC) and small male (SM) at harvest in all-male culture and to assess the growth performance of BC, OC and SM in each isolated culture. Trials involving three stocking densities of all-male prawn viz., 20, 30 and 40 juvenile m-2 were carried out in replicates. After 4 months of culture, BC, OC and SM were sorted from all tanks and restocked at 5 m-2 in treatments BC, OC and SM respectively for 80 days. A difference in prawn density significantly (p〈0.05) affected adversely on morphotypes. The highest survival rate combined with good yield performance was from 20 juvenile m-2 stocking density with 21% BC, 62.5% OC and 16.5% SM, respectively. In isolation culture, the average specific growth rate of the SM population (1.22) was significantly higher than that of the OC (1.01) and the BC (0.43) population. The survival of the SM population was 100% while for others it was 72%. Absolute weight of prawn was significantly greater in the male OC (23.87 g) than the SM (19.57 g) and the BC males (6.31 g). Impacts of isolated culture on population structure were much more pronounced in the SM population than others.
    Description: Published
    Keywords: Aquaculture ; Freshwater prawn ; Macrobrachium rosenbergii ; Heterogeneous individual growth ; Male morphotypes ; Stocking density ; Isolation culture
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.738-750
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  • 7
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    A simple and rapid method for blood collection from walking catfish, Clarias batrachus (Linneaus, 1758) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance of the caudal vein. Unfortunately, all these procedures are practically found to be slow and stressful to Clarias batrachus, including the popular caudal vein approach, likely due to the small size of caudal veins relative to the size of the species. In line with the universal ethical recommendations for taking blood from small research animals, we propose an alternative one-operator approach for C. batrachus that is simple, rapid and without the need to sacrifice the fish as with other methods. This procedure targets the dorsal aorta (a relatively larger blood vessel) in a sedated fish, punctured by inserting a needle directly from the anterior part of the anal fin about 2-5 mm behind the genital papilla, to draw the desired amount of blood. The technique is a one-operator procedure not requiring the help of an assistant or any special equipment to restrain the fish. The operation of the protocol is unique since it permits the continuous collection of blood from the same experimental fish over a varied time course and reduces the need for a large number of replicate animals. The advantages of the proposed protocol are also highlighted and discussed in detail.
    Description: Published
    Keywords: Haematology ; Catfish ; Aquaculture ; Blood sampling ; Clarias batrachus
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.935-944
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  • 8
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    A putative probiotic isolated from hatchery reared juvenile Penaeus monodon (2018)
    Details
    Publication Date: 2021-05-19
    Description: Probiotic is considered as a remedy to solve disease problem in shrimp aquaculture. Shrimp body and environment bacterial flora are recommended as reliable source for extracting of beneficial bacteria. Therefore, 5000 pieces of post larvae of Penaeus monodon were distributed equally in 4 tanks in probiotic and antibiotic free condition up to juvenile. Then microbiological samples from digestive system, muscles, body surface, rearing water and tank sediment were inoculated onto the selective media plates. Incident of total bacteria, Vibrio, Pseudomonas and Entrobacteriaceae were evaluated in different body parts and rearing environment of shrimp. The highest bacteria count for total bacteria, Vibrio, Pseudomonas was occurred in sediment followed by digestive system. No Entrobacteriaceae was observed from all samples. Isolated bacteria were identified and placed in 7 genera consisted of Vibrio, Shewanella, Burkholderia, Clavibacter, Staphylococcus, Brevibacterium, Corynebacterium, while one genus remained unidentified. Genus Shewanella exhibited highest frequent bacteria in digestive system of P. monodon which may be indicated its ability to colonization in digestive system. Shewanella algae was selected from whole isolated bacteria as candidate probiotic via well plate diffusion technique and cross streak antagonistic test against Vibrio harveyi, V. parahaemolyticus and V. alginolyticus. It was able to digest lipid and protein in tributyrin agar and skim milk agar.
    Description: Published
    Keywords: Biotechnology ; Entrobacteriaceae ; Pseudomonas ; Vibrio ; Shewanella ; Burkholderia ; Clavibacter ; Staphylococcus ; Brevibacterium ; Corynebacterium ; Vibrio harveyi ; Probiotic ; Shewanella algae ; Penaeus monodon ; Shrimp ; Juvenile ; Hatchery
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.849-866
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  • 9
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    A simple and rapid method for blood collection from walking catfish, Clarias batrachus (Linneaus, 1758) (2021)
    In:  http://aquaticcommons.org/id/eprint/23130 | 18721 | 2018-06-18 17:16:30 | 23130 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-13
    Description: Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance of the caudal vein. Unfortunately, all these procedures are practically found to be slow and stressful to Clarias batrachus, including the popular caudal vein approach, likely due to the small size of caudal veins relative to the size of the species. In line with the universal ethical recommendations for taking blood from small research animals, we propose an alternative one-operator approach for C. batrachus that is simple, rapid and without the need to sacrifice the fish as with other methods. This procedure targets the dorsal aorta (a relatively larger blood vessel) in a sedated fish, punctured by inserting a needle directly from the anterior part of the anal fin about 2-5 mm behind the genital papilla, to draw the desired amount of blood. The technique is a one-operator procedure not requiring the help of an assistant or any special equipment to restrain the fish. The operation of the protocol is unique since it permits the continuous collection of blood from the same experimental fish over a varied time course and reduces the need for a large number of replicate animals. The advantages of the proposed protocol are also highlighted and discussed in detail.
    Keywords: Biology ; Fisheries ; Haematology ; Catfish ; Aquaculture ; Blood sampling ; Clarias batrachus ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 935-944
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  • 10
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    Effect of using various amounts of patin (Pangasianodon hypophthalmus) fish oil on physical and chemical properties of moisturizing hand cream (2021)
    In:  http://aquaticcommons.org/id/eprint/26457 | 18721 | 2019-05-06 09:22:48 | 26457 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-24
    Description: Hand cream is used for rehydrating and improving skin properties. The skin is the first point of contact with the environment. Thus having healthy skin with proper barrier properties is quite important. The beneficial effects of fish oil on skin make it a favorable ingredient to be used in hand care products. In the present study, the efficacy and stability of various hand cream formulations using 0, 1.0, 2.5 and 5.0% of fish oil were investigated and compared. The appearance, odor, texture, and pH of formulations containing 1 and 2.5% of fish oil remained stable in very good qualities during the storage, whereas the related values to the appearance and texture of formulation containing 5.0% of fish oil at the end of 6 months of storage at 45°C were significantly (p〈0.05) lower than other formulations and previous evaluations of this formulation. Weight loss was not observed in all formulations under study.
    Keywords: Biology ; Chemistry ; Fisheries ; Fish oil ; Hand cream ; Formulation ; Cosmetic ; Stability ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 215-223
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