Publication Date:
2007-06-09
Description:
We describe a sensitive mRNA profiling technology, PMAGE (for "polony multiplex analysis of gene expression"), which detects messenger RNAs (mRNAs) as rare as one transcript per three cells. PMAGE incorporates an improved ligation-based method to sequence 14-nucleotide tags derived from individual mRNA molecules. One sequence tag from each mRNA molecule is amplified onto a separate 1-micrometer bead, denoted as a polymerase colony or polony, and about 5 million polonies are arrayed in a flow cell for parallel sequencing. Using PMAGE, we identified early transcriptional changes that preceded pathological manifestations of hypertrophic cardiomyopathy in mice carrying a disease-causing mutation. PMAGE provided a comprehensive profile of cardiac mRNAs, including low-abundance mRNAs encoding signaling molecules and transcription factors that are likely to participate in disease pathogenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Jae Bum -- Porreca, Gregory J -- Song, Lei -- Greenway, Steven C -- Gorham, Joshua M -- Church, George M -- Seidman, Christine E -- Seidman, J G -- New York, N.Y. -- Science. 2007 Jun 8;316(5830):1481-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cardiovascular Division, Brigham and Women's Hospital, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17556586" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cardiomyopathy, Hypertrophic/*genetics/pathology/physiopathology
;
DNA, Complementary
;
Fibrosis/genetics/pathology
;
Gene Expression Profiling/*methods
;
*Gene Expression Regulation
;
Gene Library
;
Heart Ventricles/metabolism
;
Mice
;
Mutation
;
Myocardial Contraction
;
Myocardium/*metabolism
;
Myosin Heavy Chains/genetics
;
RNA, Messenger/genetics/metabolism
;
Reproducibility of Results
;
Sensitivity and Specificity
;
Sequence Analysis, DNA
;
Templates, Genetic
;
Transcription Factors/genetics
;
*Transcription, Genetic
;
Ventricular Myosins/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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