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  • Springer  (31)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation and properties of a Lyt-2.1-negative mutant of a Lyt-2.1/Lyt-2.2 CTL line (1991)
    Springer
    Immunogenetics 34 (1991), S. 42-51 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212311
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  • 2
    Electronic Resource
    Electronic Resource
    Immunoglobulin classes and subclasses in alloantisera to mouse thymocyte surface antigens (1979)
    Springer
    Immunogenetics 8 (1979), S. 517-528 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A variety of cytotoxic alloantisera directed against murine cell surface components were analyzed for their content of specific IgG1, IgG2a, and IgG2b antibodies by radioimmunoassay, and specific IgM antibodies by 2-mercaptoethanol inhibition of cytotoxicity. Results indicate: (1) that the IgM and IgG subclass content of specific antibodies in anti-Lyt antisera produced at M.I.T. and Sloan-Kettering using the same donor and recipient strains are similar; (2) that specific antibodies produced concurrently in the same mice against at least two different alloantigens (Lyt-3.1 and H-2k) can differ in IgG subclass content; and (3) that preparation of alloantisera against a given antigen (Lyt-3.1) using different combinations of donor and recipient strains can yield specific antibodies which differ in IgG subclass and cytotoxic IgM content. Thus, it appears that humoral immune responses to cell surface alloantigens are not random, but reflect both the antigens being recognized and the strains employed for the immunization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01561461
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  • 3
    Electronic Resource
    Electronic Resource
    Nucleotide sequence analysis of the C.AKR Lyt-2 agene: structural polymorphism in alleles encoding the Lyt-2.1 T-cell surface alloantigen (1988)
    Springer
    Immunogenetics 28 (1988), S. 345-352 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The Lyt-2 aallele of the C.AKR strain of mice (genotype Lyt-2 a, Lyt-3 a) was cloned, and its complete nucleotide sequence as well as that of 2 kb of 5′ flanking DNA was determined. The sequence was comapred with the partial sequence of the Lyt-2 aallele of DBA/2 (genotype Lyt-2 a, Lyt-3 b) and the nearly complete sequence of the B10.CAS2 Lyt-2 ballele reported by Liaw and coworkers (1986). The coding regions of the two Lyt-2 aalleles differ from each other by two nucleotide substitutions in the three exons over which they could be compared, resulting in two amino acid substitutions in the leader and transmembrane segments. The coding region of the C.AKR Lyt-2 aallele differs from the Lyt-2 ballele by two nucleotide substitutions in the extracellular V-like domain, one of which is silent and the second of which leads to substitution of valine for methionine at amino acid position 78 giving rise to the Lyt-2.1 allotypic specificity. The coding region of the DBA/2 Lyt-2 aallele shares with C.AKR the allotypic substitution at position 78 and differs from Lyt-2 bby three additional nucleotide substitutions in the coding regions, two of which lead to amino acid substitutions in the leader and transmembrane segments. It would therefore appear that the Lyt-2 alleles of the three strains analyzed are distinct, and the nomenclature Lyt-2 a1 and Lyt-2 a2 is suggested to distinguish the alleles of C.AKR and DBA/2, respectively. These alleles share a common difference from the Lyt-2 bgene product at position 78, and since the amino acid substitutions which distinguish them from each other are in the leader and transmembrane segments, their mature Lyt-2 gene products appear antigenically identical.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364233
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  • 4
    Electronic Resource
    Electronic Resource
    Structure and expression of the Lyt-3 agene of C.AKR mice (1988)
    Springer
    Immunogenetics 28 (1988), S. 353-361 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mouse Lyt-3 agene, which encodes the Lyt-3.1 T-cell surface alloantigen of the C.AKR strain, has been cloned, and the nucleotide sequence of its exons and more than 2 kb of 5′ flanking sequence have been determined. The gene extends over approximately 16 kb of DNA and consists of six exons encoding leader, leader plus V-like domain, membrane-proximal, transmembrane, and cytoplasmic domains. The only difference between the coding region of the Lyt-3 agene and the cDNA sequences reported for Lyt-3 b(Nakauchi et al. 1987, Panaccio et al. 1987) is at position 77 of the mature protein where Lyt-3 aencodes serine and Lyt-3 bencodes arginine. This substitution must therefore be the basis for the serological distinction between the Lyt-3.1 and Lyt-3.2 alloantigens. Potential TATA and CAAT sequences, two Sp1 protein binding sites, two extended repeats of the dinucleotide, CA, a number of short inverted repeats, and an inverted segment of the mouse B1 repetitive sequence are found 5′ to the Lyt-3 agene. Two consensus poly-A addition signals and a complete copy of the mouse B1 sequence are found 3′ to the gene. Both B1-related regions are flanked by short direct repeats suggesting that they arose by an insertional mechanism. Cotransfection of the Lyt-3 agene together with a cloned Lyt-2 agene resulted in expression of both Lyt-2 and Lyt-3.1 on the surface of Ltk− and BW5147 cells. Transfection of the Lyt-3 agene without Lyt-2 aled to expression of Lyt-3-related cellular RNA but did not result in surface expression of Lyt-3.1, suggesting that the Lyt-3 glycoprotein is not expressed on the cell surface in the absence of Lyt-2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364234
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  • 5
    Electronic Resource
    Electronic Resource
    Structural and evolutionary comparisons of four alleles of the mouse immunoglobulin kappa chain gene,Igk-VSer (1989)
    Springer
    Immunogenetics 29 (1989), S. 249-257 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mouseIgK-VSer gene encodes an immunoglobulin κ light chain variable region which gives rise to two phenotypic polymorphisms of mouse κ chains. The nucleotide sequences of coding and flanking regions of theIgk-VSer c andIgk-VSer d alleles found in recently inbred strains of wild mice are compared with those of theIgk-VSer a andIgk-VSer b alleles described previously. Results suggest that the gene is evolving randomly and that framework 2 and complentarity determining region 2 are preserved, presumably for overall light chain structure. Results indicate that all four allels have an octamer motif upstream of the gene which should be functional and allow prediction of whether or not the product of the germ line gene will be detectable as either the IB-peptide or Ef1a phenotypic polymorphism. Southern hybridization of genomic DNA using as probe a 1-kbXba I-Xba I fragment located approximately 4 kb upstream of the BALB/cIgk-VSer b coding region demonstrated the presence of homologous DNA in mice bearing theIgk-VSer a allele and absence from mice bearing theIgk-VSer c andIgk-VSer d alleles. Nucleotide sequence comparison of BALB/c and SK/CamRk (Igk-VSer d ) DNA in this region demonstrated that BALB/c contained an insertion 2.4 kb in length which was absent from SK/CamRk. Both strains contain DNA homologous to the reverse complement of the mouse Bam5 repetitive element at the point of the insertion, with BALB/c containing approximately 70 nucleotides more of the element than SK/CamRk. Surprisingly, the strains containing DNA related to theXba I-Xba I probe are not those determined to be the most similar by nucleotide sequence comparisons and by the Phylogenetic Analysis Using Parsimony program. The evolutionary relationship of the alleles and a possible basis for the inconsistency presented by theXba I-Xba I fragment-related DNA are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00717909
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  • 6
    Electronic Resource
    Electronic Resource
    Structural and evolutionary comparisons of four alleles of the mouse Igk-J locus which encodes immunoglobulin kappa light chain joining (J K ) segments (1989)
    Springer
    Immunogenetics 29 (1989), S. 389-396 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The Igk-J locus of the mouse encodes the immunoglobulin κ light chain joining (J) segments. Four Igk-J alleles have been described on the basis of restriction enzyme length polymorphisms. The nucleotide sequences of the Igk-J a allele (type strain, C.C58), Igk-J c allele (type strain, SJL/J), and Igk-J d allele (type strain, SK/CamRk) have been determined and are compared with the previously reported Igk-J b allele sequence (type strain, BALB/c). The mouse sequences are also compared with published sequences for rat and human J k sequences. Far more differences were found between the Igk-J a allele and the other mouse alleles than between any two of the latter. These result in two amino acid substitutions which distinguish the J2 and J3 1 segments of the Igk-J a allele from the other three alleles. Use of the Phylogenetic Analysis Using Parsimony program to generate a phylogenetic tree strongly indicates that after divergence from the rat ancestor, there appears to have been an early split between the Igk-J a allele and the evolutionary precursor of the other mouse alleles. There also appears to have been far less divergence from the ancestral condition in the Igk-J a allele than in the other alleles. Also, the presence of only one convergent mutation among the four mouse alleles provides strong evidence against any crossing over within the Igk-J locus during the history of these alleles. Finally, the differences in rates of evolution of the Igk-J alleles are in marked contrast to the relatively uniform rates of divergence of four alleles of a mouse V k gene, Igk-VSer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00375867
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  • 7
    Electronic Resource
    Electronic Resource
    Production of congenic Anti-Lyt-3.1 sera (1978)
    Springer
    Immunogenetics 7 (1978), S. 165-168 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01844002
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  • 8
    Electronic Resource
    Electronic Resource
    Control of the antibody response of C57BL/6 Lyt-congenic strains to the Lyt-3.1 alloantigen by a gene linked to theLyt-2 locus (1980)
    Springer
    Immunogenetics 10 (1980), S. 227-234 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Congenic anti-Lyt-3.1 sera have recently been produced by immunizing B6-Lyt-2a mice with thymocytes from either B6-Lyt-2a, Lyt-3a or B6-Lyt-2a, Lyt-3a, H-2k mice (Boos et al. 1978). Surprisingly, mice of the congenic strain B6 failed to produce either anti-Lyt-2.1 or anti-Lyt-3.1 cytotoxic antibodies after identical immunizations. To determine the genetic basis for the difference in response to Lyt-3.1, (B6 × B6-Lyt-2a)Fa mice and progeny of the backcross, (B6 × B6-Lyt-2a)F1 × B6-Lyt-2a, were immunized with B6-Lyt-2a, Lyt-3a, H-2k thymocytes. In addition, thymic biopsies of backcross progeny were performed and thymocytes tested for the Lyt-2.2 antigenic specificity. Results indicate that gene(s) governing the immune response to Lyt-3.1 is (are) linked to theLyt-2 locus, and that the responder allele (linked toLyt-2 a ) shows very poor penetrance in Lyt-2a/Lyt-2b mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01561571
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  • 9
    Electronic Resource
    Electronic Resource
    Construction and properties of new Lyt-congenic strains and anti-Lyt-2.2 and anti-Lyt-3.1 monoclonal antibodies (1980)
    Springer
    Immunogenetics 10 (1980), S. 545-555 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Hybridomas producing mouse monoclonal IgM antibodies specific for Lyt-2.2 and Lyt-3.1 T-cell surface alloantigens have been constructed. Cytotoxic titers of ascites fluids were found to be 10−6 or greater and no lysis of thymocytes of congenic strains bearing the alternative allele was observed at the lowest dilutions tested (1∶2). The anti-Lyt-2.2 monoclonal antibody (HO-2.2) specifically precipiated from extracts of Lyt-2.2-positive thymocytes molecular species indistinguishable from those precipitated by conventional anti-Lyt-2.2 sera. However, by immunoprecipitation criteria (though not by cytotoxicity), the anti-Lyt-3.1 antibody (HO-3.1) demonstrated some cross-reactivity with similar molecular species from Lyt-3.1-negative thymocytes. In addition, three new strains of mice differing from existing strains in the region of theLyt-2 and4Lyt-3 loci have been constructed. They are: C.C58-Lyt-2a, Lyt-3a and C.AKR-Lyt-2a, Lyt-3a, congenic with Balb/cAn and bearingLyt-2 a andLyt-3 a alleles of C58/J and AKR/J, respectively; and AKR.C-Lyt-2b, Lyt-3b, congenic with AKR/J and bearing theLyt-2 b andLyt-3 b alleles of Balb/cJ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01572589
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  • 10
    Electronic Resource
    Electronic Resource
    Linkage of a 7S RNA sequence and kappa light chain genes in the mouse (1985)
    Springer
    Immunogenetics 22 (1985), S. 471-481 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A mouse 7S RNA cDNA plasmid clone was employed to identify and map DNA restriction fragment variants using recombinant inbred (RI) and congenic mouse strains. More than a dozen such restriction variants were identified and mapped to different regions of the mouse genome. One such variant, designated Rn7s-6, showed close linkage to the Ly-2,3-Igk-V (T lymphocyte antigens 2 and 3, kappa immunoglobulin variable region) cluster of markers on chromosome 6. No recombinants were detected among three of these markers in 59 RI strains. On the basis of these data, the Rn7s-6 sequence may be placed within 1.3 centimorgans of Ly-3 and one of the Igk-V-region markers, Igk-Efl. Two mouse stocks with previously identified crossovers within the Ly2,3-Igk-V region were used to sublocalize Rn7s-6. The results are consistent with the gene order (Ly-2, Ly-3)-(Rn7s-6, Igk-Efl)-Igk-Ef2. Several mouse plasmacytomas, known to have various parts of the kappa chain complex deleted, retain the Rn7s-6 sequence. The Rn7s-6 variant is a plus/minus variant; no sequence allelic to Rn7s-6 is found in inbred strains that share the Ly-3 a-Igk-Efla haplotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00418092
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