Abstract
The mouseIgK-VSer gene encodes an immunoglobulin κ light chain variable region which gives rise to two phenotypic polymorphisms of mouse κ chains. The nucleotide sequences of coding and flanking regions of theIgk-VSer c andIgk-VSer d alleles found in recently inbred strains of wild mice are compared with those of theIgk-VSer a andIgk-VSer b alleles described previously. Results suggest that the gene is evolving randomly and that framework 2 and complentarity determining region 2 are preserved, presumably for overall light chain structure. Results indicate that all four allels have an octamer motif upstream of the gene which should be functional and allow prediction of whether or not the product of the germ line gene will be detectable as either the IB-peptide or Ef1a phenotypic polymorphism. Southern hybridization of genomic DNA using as probe a 1-kbXba I-Xba I fragment located approximately 4 kb upstream of the BALB/cIgk-VSer b coding region demonstrated the presence of homologous DNA in mice bearing theIgk-VSer a allele and absence from mice bearing theIgk-VSer c andIgk-VSer d alleles. Nucleotide sequence comparison of BALB/c and SK/CamRk (Igk-VSer d) DNA in this region demonstrated that BALB/c contained an insertion 2.4 kb in length which was absent from SK/CamRk. Both strains contain DNA homologous to the reverse complement of the mouse Bam5 repetitive element at the point of the insertion, with BALB/c containing approximately 70 nucleotides more of the element than SK/CamRk. Surprisingly, the strains containing DNA related to theXba I-Xba I probe are not those determined to be the most similar by nucleotide sequence comparisons and by the Phylogenetic Analysis Using Parsimony program. The evolutionary relationship of the alleles and a possible basis for the inconsistency presented by theXba I-Xba I fragment-related DNA are discussed.
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Ponath, P.D., Hillis, D.M. & Gottlieb, P.D. Structural and evolutionary comparisons of four alleles of the mouse immunoglobulin kappa chain gene,Igk-VSer . Immunogenetics 29, 249–257 (1989). https://doi.org/10.1007/BF00717909
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DOI: https://doi.org/10.1007/BF00717909