Skip to main content
SpringerLink
Log in

Isolation and properties of a Lyt-2.1-negative mutant of a Lyt-2.1/Lyt-2.2 CTL line

  • Brief Communications
  • Published:
Immunogenetics Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Barber, E. K., Dasgupta, J. D., Schlossman, S. F., Trevillyan, J. M., and Rudd, C.: The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex. Proc Natl Acad Sci USA 86: 3277–3281, 1989

    Google Scholar 

  • Boyse, E. A., Itakura, K., Stockert, E., Iritani, C. A., and Miura, M.: LyC: a third locus specifying alloantigens exposed only on thymocytes and lymphocytes. Transplantation 11: 351–352, 1971

    Google Scholar 

  • Connolly, J. M., Potter, T. A., Wormstall, E.-M., and Hansen, T. H.: The Lyt-2 molecule recognizes residues in the class I a3 domain in the allogeneic cytotoxic T-cell response. J Exp Med 168: 325–341, 1988

    Google Scholar 

  • Dembic, Z., Haas, W., Zamoyska, R., Parnes, J., Steinmetz, M., and von Boehmer, H.: Transfection of the CD8 gene enhances T-cell recognition. Nature 326: 510–511, 1987

    Google Scholar 

  • Dialynas, D. P. and Fitch, F. W.: Lyt-2-/Lyt-3-variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis. J Exp Med 153: 595, 1981

    Google Scholar 

  • Emmrich, F., Kanz, L., and Eichmann, K.: Cross-linking of the T-cell receptor complex with the subset-specific differentiation antigen stimulates interleukin 2 receptor expression in human CD4 and CD8 T-cells. Eur J Immunol 17: 529–534, 1987

    Google Scholar 

  • Evans, G. A., Margulies, D. H., Shykind, B., Seidman, J. G., and Ozato, K.: Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens. Nature 300: 755–757, 1982

    Google Scholar 

  • Fleischer, B., Schrezenmeier, H., and Wagner, H.: Function of the CD4 and CD8 molecules on human cytotoxic T lymphocytes: regulation of cell triggering. J Immunol 136: 1625–1628, 1986

    Google Scholar 

  • Gabert, J., Langlet, C., Zamoyska, R., Parnes, J. R., Pchmitt-Verhulst, A.-M., and Malissen, B.: Reconstitution of the MHC class I specificity by transfer of the T-cell receptor and Lyt-2 genes. Cell: 50: 545–554, 1987

    Google Scholar 

  • Glasebrook, A. L. and Fitch, F. W.: Alloreactive cloned T-cell lines. I. Interactions between cloned amplifier and cytolytic T-cell lines. J Exp Med 151: 876, 1980

    Google Scholar 

  • Glasebrook, A. L., Wild, D. B., Marrack, P., Pierres, A., Wall, K. A., Havran, W., Otten, G., Loken, M. R., Pierres, M., Kappler, J., and Fitch, F. W.: Characteristics of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK 1.5: expression of L3T4a by functional T-cell clones appears to correlate primarily with class II MHC antigen reactivity. Immunol Rev 74: 29, 1983

    Google Scholar 

  • Gottlieb, P. D., Marshak-Rothstein, A., Auditore-Hargreaves, K., Berkoben, D. B., August, D. A., Roche, R. M., and Benedetto, J. D.: Construction and properties of new Lyt-congenic strains and anti-Lyt-2.2 and anti-Lyt-3.1 monoclonal antibodies. Immunogenetics 10: 545–555, 1980

    Google Scholar 

  • Hasenkrug, K. J., Cory, J. M., and Stimpfling, J. H.: Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region. Immunogenetics 25: 136–139, 1987

    Google Scholar 

  • Hunig, T.: Monoclonal anti-Lyt-2.2 antibody blocks lectin-dependent cellular cytotoxicity of H-2 negative target cells. J Exp Med 159: 537, 1984

    Google Scholar 

  • Klein, J., Figueroa, F., and David, C. S.: H-2 haplotypes, genes and antigens: second listing. Immunogenetics 17: 553–596, 1983

    Google Scholar 

  • Ledbetter, J. A. and Herzenberg, L. A.: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 63–90, 1979

    Google Scholar 

  • Ledbetter, J. A., Seaman, W. E., Tsu, T. T., and Herzenberg, L. A.: Lyt-2 and Lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytotoxic activity. J Exp Med 153: 1503–1516, 1981

    Google Scholar 

  • Ledbetter, J. A., June, C. H., Grosmaire, L. S., and Rabinovitch, P. S.: Crosslinking of surface antigens causes mobilization of intracellular ionized calcium in T lymphocytes. Proc Natl Acad Sci USA 84: 1384–1388, 1987

    Google Scholar 

  • Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., and Bluestone, J. A.: Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci USA 84: 1374–1378, 1987

    Google Scholar 

  • Liaw, C. W., Zamoyska, R., and Parnes, J. R.: Structure, sequence and polymorphism of the Lyt-2 T cell differentiation gene. J Immunol 137: 1037–1043, 1986

    Google Scholar 

  • Littman, D. R.: The structure of the CD4 and CD8 genes. Annu Rev Immunol 5: 561–584, 1987

    Google Scholar 

  • MacDonald, H. R., Glasebrook, A. L., and Cerottini, J.-C.: Clonal heterogeneity in the functional requirement for Lyt-2/3 molecules on cytolytic T lymphocytes: Analysis by antibody blocking and selective trypsinization. J Exp Med 156: 1711–1722, 1982

    Google Scholar 

  • Munakata, T., Schmidt-Ullrich, R., Bertschmann, M., and Eichmann, K.: Role of CD8 (Lyt-2) in cytotoxic T-cell function: Analysis of variants of a cytotoxic T-cell clone with reduced Lyt-2 expression. Scand J Immunol 28: 75–80, 1988

    Google Scholar 

  • Norment, A. M., Salter, R. D., Parham, P., Englehard, V. H., and Littman, D. R.: Cell-cell adhesion mediated by CD8 and MHC class I molecules. Nature 336: 79, 1988

    Google Scholar 

  • Ozato, K., Hansen, T. H., and Sachs, D. H.: Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polypeptide locus of the mouse major histocompatibility complex. J Immunol 125: 2473–2477, 1980

    Google Scholar 

  • Palladino, M. A., Ranges, G. E., Oettgen, H. F., and Old, L. J.: Functional Relationships of Lyt-2 and Lyt-3 expression and T-cell cytotoxicity: a new model system. Adv Exp Med Biol 184: 355–364, 1984

    Google Scholar 

  • Potter, T. A., Rajan, T. V., Dick, R. F., II, and Bluestone, J. A.: Substitution at residue 227 of H-2 class I molecules abrogates recognition by CD8-dependent, but not CD8-independent, cytotoxic T lymphocytes. Nature 337: 73–75, 1989

    Google Scholar 

  • Rajan, T. V. and Halay, E. D.: Anti-H-2 hybridoma antibodies as immunoselective agents. I. Isolation of forward and reverse mutations for reactivity with a monoclonal antibody. Immunogenetics 14: 253–262, 1981

    Google Scholar 

  • Rajan, T. V. and Halay, E. D.: H-2 antigen variants in a cultures heterozygous mouse leukemia cell line. Immunogenetics 10: 411–422, 1980

    Google Scholar 

  • Saizawa, K., Rojo, J., and Janeway, C. A.: Evidence for a physical association of CD4 and the CD3: a, b T-cell receptor. Nature 328: 260–263, 1987

    Google Scholar 

  • Salter, R. D., Norment, A. M., Chen, B. P., Clayberger, C., Krensky, A. M., Littman, D. R., and Parham, P.: Polymorphism in the α3 domain of HLA-A molecules affects binding to CD8. Nature 338: 345–347, 1989

    Google Scholar 

  • Sarmiento, M., Glasebrook, A., and Fitch, W.: IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement. J Immunol 125: 2665–2672, 1980

    Google Scholar 

  • Shinohara, N. and Sachs, D.: Mouse alloantibodies capable of blocking cytolytic T-cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus.J Exp Med 150: 432–444, 1979

    Google Scholar 

  • Swain, S. L.: T cell subsets and the recognition of MHC class. Immunol Rev 74: 129–142, 1983

    Google Scholar 

  • Takada, S. E. and Engleman, E. G.: Evidence for an association between CD8 molecules and the T-cell receptor complex on cytotoxic T cells. J Immunol 139: 3231–3235, 1987

    Google Scholar 

  • Van Seventer, G. A. V., van Lier, R. A. W., Spits, H., Ivanyi, P., and Melief, C. J. M.: Evidence for a regulatory role of the T8 (CD8) antigen in ntigen-specific and anti-T3-(CD3-)induced lytic activity of allospecific and anti-T3-(CD3-)induced lytic activity of allospecific CTL clone. Eur J Immunol 16: 1363–1371, 1986

    Google Scholar 

  • Veillette, A., Bookman, M. A., Horak, E. M., and Bolen, J. B.: The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase. Cell 55: 301–308, 1988

    Google Scholar 

  • Youn, Hyun J., Harriss, June V., and Gottlieb, Paul D.: Nucleotide sequence analysis of the C.AKR Lyt-2 agene: Structural polymorphism is alleles encoding the Lyt-2.1 T-cell surface alloantigen. Immunogenetics 28: 345–352, 1988a

    Google Scholar 

  • Youn, H. J., Harriss, J. V., and Gottlieb, P. D.: Structure and expression of the Lyt-3 agene of C.AKR mice. Immunogenetics 28: 353–361, 1988b

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Texas at Austin, 78712, Austin, TX, USA

    Zy-Ting E. Chu & Paul D. Gottlieb

  2. Departments of Microbiology, University of Texas Health Sciences Center at San Antonio, 78284, San Antonio, TX, USA

    John T. Kung & Charles Thomas III

  3. Departments of Biochemistry, University of Texas Health Sciences Center at San Antonio, 78284, San Antonio, TX, USA

    Katherine A. Wall

Authors
  1. Zy-Ting E. Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John T. Kung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Charles Thomas III
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Katherine A. Wall
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Paul D. Gottlieb
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chu, ZT.E., Kung, J.T., Thomas, C. et al. Isolation and properties of a Lyt-2.1-negative mutant of a Lyt-2.1/Lyt-2.2 CTL line. Immunogenetics 34, 42–51 (1991). https://doi.org/10.1007/BF00212311

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00212311

Keywords

Search

Navigation