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  • 1985-1989  (23)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 42 (1989), S. 682-686 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 97 (1986), S. 309-316 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract We describe a radioimmunoassay procedure for human somatotropin using second antibody — polyethylene glycol (PEG) combination for the separation of antibody bound and free antigen. The assay is done by a single incubation of 18 hours or with one hour pre-incubation and 3 hours post-incubation at room temperature (25 °C) and uses 0.1 mℓ of serum sample. The assay covers a range of 0–40 μg/ℓ and has a sensitivity of 0.6 μg/ℓ of somatotropin. The assay is validated by inter-assay and intra-assay variations, recovery and parallelism tests.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 32 (1987), S. 293-302 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Rain water samples were regularly collected from three sites, namely, Netaji Nagar, Moti Nagar and Town Hall within the Delhi city area from July, 1980 to June, 1982. The pesticide residues were adsorbed on polyurethane foam coated with 0.5 % DC-200 and subsequently extracted and analyzed for DDT, HCH and their metabolites/isomers. The concentration of total DDT ranged from 0.22 to 108 μg L−1 with a mean value of 12.5 μg L−1. The samples of rain water contained varying levels of 4,4-DDT, 2,4-DDT, 4,4-DDE, and 4,4-DDD. The 4,4-DDT, 4,4-DDE and 2,4-DDT were the main components of total DDT. The range of HCH residues in rain water was from 0.08 to 43 μg L−1 with a mean of 5.3 μg L−1. The residues of HCH consisted mainly of α- and γ-isomers with traces of β and δ-isomers. The α- and γ-isomers accounted for 76 and 24% of total HCH, respectively. The concentrations of DDT and HCH in rain water were generally less than 10 μg L−1 and exceeded 10 in only 4 and 3 cases, respectively. The residues of these insecticides were generally higher during October to December. Residues of DDT were higher at Moti Nagar which is near a DDT factory. Residues of HCH were maximum at Town Hall, a commercial area of the city.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 28 (1986), S. 89-104 
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract DDT residues in water, bottom sediments and certain non-target organisms from four different sites of the river Jamuna in Delhi were monitored periodically from 1976 to 1978. All the samples contained DDT residues in varying concentrations. The concentrations of total DDT residues ranged from 0.04 to 3.42 μg L−1 in water, 0.007 to 5.63 mg kg−1 in bottom sediments, 0.05 to 15.24 mg kg−1 in various invertebrates and 0.54 to 56.31 mg kg−1 in different fish. The total DDT concentration was comparatively higher at the downstream Wazirabad site where there is a mixing of river water with the discharge from the Najafgarh drain which also carries effluents of a DDT factory along with that of other industries. No significant correlation was found between total DDT concentration obtained in water with those found in sediments collected from the same sites. Among the DDT residues, p,p′-DDT and p,p′-DDE were more predominant, being detected in most of the samples while p,p′-DDD and o,p′-DDT were detected in a lesser number of samples.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A single reagent radioimmunoassay for thyroxine in blood samples absorbed on filter paper for the mass screening of neonatal hypothyroidism is described. Blood samples were collected by pricking the heel of newborn babies (3 days old) and pressing Whatman 3 filter paper against the wound. 6 mm diameter blood spots were punched out at the time of assay and incubated with 0.4 ml of a preincubated antigen-antibody complex for six hours at 37 °C. 1 ml of 22% polyethylene glycol is used for the precipitation of antigen-antibody complex. The assay has a sensitivity of 2.2 ng/ml. 500 samples collected from newborns were analyzed in the assay and gave a mean of 117.6±31.9 ng/ml.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 102 (1986), S. 435-441 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract We discribe the development of a simplified radioimmunoassay for triiodothyronine (T3) using pre-incubated labelled T3 and antibody. The assay is carried out by adding 50 μl of standard or sample to 0.4 ml of pre-incubated reagent dispensed in assay tubes. The reaction is allowed to proceed for about four hours and the antigen-antibody complex precipitated by the addition of 1 cm3 of 22% polyethylene glycol solution. Due to the high dissociation constant of T3-antibody complex at 37° C (2.83·10−4 S−1), the labelled antigen-antibody complex dissociates and thereby the unlabelled antigen binds with the antibody. With a four hour incubation the sensitivity of this assay is comparable to an assay done by the equilibrium method using the same antibody. Sixty serum samples were analyzed using this method and compared with the equilibrium assay (Y=0.94x+0.046 ng/cm3, r=0.98).
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Radioiodinated insulin and thyroid stimulating hormones were prepared using 1,3,4,6-tetrachloro-3α, 6α-diphenylglycouril (Iodogen). Conditions of iodination like concentration of iodogen, reaction time, etc., were optimized to get maximum yield. Stability studies of iodogen coated tubes were carried out over a period of time. The dependence of iodination yield on varying amounts of activity and protein concentration were investigated. Iodination yield over a range of pH was also studied. The radiolabelled hormones prepared by this method were used in radioimmunoassay and were compared with tracers prepared by the Chloramine-T method.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 102 (1986), S. 429-434 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A solid phase radioimmunoassay for triiodothyronine (T3) has been developed using antibody-immobilized serum albumin microspheres. Antibody albumin microspheres were prepared using a “spinning disc aerosol generator”. The low density of the antibody-albumin microspheres gives greater mobility for the particles there by ensuring better kinetics to the antigen-antibody reaction. The assay has a single incubation of one hour at 37° C and the separation of the antigen-antibody complex is accomplished by centrifugation. The sensitivity of this assay is 0.3 ng/cm3 and has a range of 0.3–4 ng/cm3.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 97 (1986), S. 45-49 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A simple and reliable technique for the simultaneous estimation of serum triiodothyronine (T3) and thyroxine (T4) is discussed. T3 assay was done by the solid phase technique using antibody coated Eppendorf pipette tips. T4 assay was done by the polyethylene glycol separation system. The assay used 50 μl of serum sample. Inter-assay and intra-assay coefficient of variation are less than 12% throughout the assay range, for both assays.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 116 (1987), S. 317-323 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The paper describes a solid-phase radioimmunoassay for triiodothyronine (T3) using antibody coupled to carboxymethylcellulose powder. The free carboxylic acid groups of cellulose are covalently coupled to the amino groups of the antibody using 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride. Immobilized antibody thus prepared was used in a solid-phase radioimmunoassay of T3. The assay has a sensitivity of 0.18 μg/l, and a range of 0.18–4 μg/l. Satisfactory correlation was obtained when this assay was compared with a T3 assay based on dextran coated charcoal separation system (Y=0.95X+0.15 μg/L; r=0.98).
    Type of Medium: Electronic Resource
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