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A solid phase radioimmunoassay for triiodothyronine using antibody-immobilized serum albumin microspheres

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Abstract

A solid phase radioimmunoassay for triiodothyronine (T3) has been developed using antibody-immobilized serum albumin microspheres. Antibody albumin microspheres were prepared using a “spinning disc aerosol generator”. The low density of the antibody-albumin microspheres gives greater mobility for the particles there by ensuring better kinetics to the antigen-antibody reaction. The assay has a single incubation of one hour at 37° C and the separation of the antigen-antibody complex is accomplished by centrifugation. The sensitivity of this assay is 0.3 ng/cm3 and has a range of 0.3–4 ng/cm3.

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Authors and Affiliations

  1. ISOPHARM, Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Turbhe Complex, 400 705, New Bombay, India

    M. R. A. Pillai, J. H. Gupte & R. S. Mani

Authors
  1. M. R. A. Pillai
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  2. J. H. Gupte
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  3. R. S. Mani
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Pillai, M.R.A., Gupte, J.H. & Mani, R.S. A solid phase radioimmunoassay for triiodothyronine using antibody-immobilized serum albumin microspheres. Journal of Radioanalytical and Nuclear Chemistry, Articles 102, 429–434 (1986). https://doi.org/10.1007/BF02047917

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  • DOI: https://doi.org/10.1007/BF02047917

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