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Solid phase radioimmunoassay of triiodothyronine (T3) using antibody coupled carboxymethylcellulose powder

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Abstract

The paper describes a solid-phase radioimmunoassay for triiodothyronine (T3) using antibody coupled to carboxymethylcellulose powder. The free carboxylic acid groups of cellulose are covalently coupled to the amino groups of the antibody using 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride. Immobilized antibody thus prepared was used in a solid-phase radioimmunoassay of T3. The assay has a sensitivity of 0.18 μg/l, and a range of 0.18–4 μg/l. Satisfactory correlation was obtained when this assay was compared with a T3 assay based on dextran coated charcoal separation system (Y=0.95X+0.15 μg/L; r=0.98).

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Authors and Affiliations

  1. Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, 400 085, Bombay, (India)

    M. R. A. Pillai, Jyotsna Narayanan, J. H. Gupte & R. S. Mani

Authors
  1. M. R. A. Pillai
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  2. Jyotsna Narayanan
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  3. J. H. Gupte
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  4. R. S. Mani
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Pillai, M.R.A., Narayanan, J., Gupte, J.H. et al. Solid phase radioimmunoassay of triiodothyronine (T3) using antibody coupled carboxymethylcellulose powder. Journal of Radioanalytical and Nuclear Chemistry, Articles 116, 317–323 (1987). https://doi.org/10.1007/BF02035775

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  • DOI: https://doi.org/10.1007/BF02035775

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