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  • 1
    Electronic Resource
    Electronic Resource
    Flavanone and Nootkatone Levels in Different Varieties of Grapefruit and Pummelo (1995)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 43 (1995), S. 1-5 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00049a001
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of benzylaminopurine on the flavanones hesperidin, hesperetin 7-O-glucoside and prunin in tangelo nova fruits. (1995)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 43 (1995), S. 2030-2034 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00056a014
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  • 3
    Electronic Resource
    Electronic Resource
    Distribution of indole-3-acetic acid in relation to the growth of etiolated Lupinus albus hypocotyls (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 66 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The free indole-3-acetic acid (IAA) in methanolic extracts of etiolated hypocotyls of lupin (Lupinus albus L., from Bari, Italy) was determined by fluorimetry. The distribution of IAA along the hypocotyls was parallel to the growth, but when growth ceased oscillations occurred in the auxin level. These oscillations could be related to processes of differentiation mediated by IAA. The oscillations did not obey any impulses from the apex, since the application of [1-14C]-IAA to decapitated plants gives a distribution of radioactivity which also presents an undulatory pattern. Our results support the hypothesis that morphogenesis can be regulated by information transmitted by the translocation of waves of auxin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb05959.x
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in the concentration of indole-3-acetic acid during the growth of etiolated lupin hypocotyls (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 78 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The longitudinal distribution of unaltered radioactive indole-3-acetic acid (IAA), after application of [5-3H]-IAA to decapitated etiolated lupin hypocotyls. exhibited a wave-like pattern similar to that obtained with endogenous IAA. Waves of radioactive IAA were localizated both in the elongation zone and in the non-growing basal region of the hypocotyl. These IAA waves were transient because of basipetal polar transport and metabolism of IAA.The level of endogenous IAA in different zones of the hypocotyl varied with age, following a wave-like pattern. During the elongation period of each zone, IAA was parallel to the bell-shaped curve of the growth rate. In addition, a role in secondary cell wall deposition is suggested for the other IAA wave that appeared after the cell elongation period, since an electron microscopic morphometric analysis of the cell wall showed that the cell wall thickness increased once the cell elongation ceased.As the oscillation of endogenous IAA level occured in both space (distribution along the hypocotyl) and time (variation with age), it is suggested that the level of IAA really depended on the growth status of the cells. The response of the cells to the positional information submitted by the auxin waves as regards the growth status of the cell is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb02083.x
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  • 5
    Electronic Resource
    Electronic Resource
    Influence of ethylene on apex development and mobilization of soluble saccharides in the corm of Liatris (1997)
    Springer
    Biologia plantarum 39 (1997), S. 197-206 
    Details
    ISSN: 1573-8264
    Keywords: ethephon ; flower initiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the influence of ethephon (2-chloroethylphosphonic acid) on the first stages of development of the main apex of Liatris spicata cv. Callilepis and its relation to the mobilization of soluble saccharides in the corm. Ethephon enhanced cell expansion during the first weeks of cultivation but did not affect the timing of flower initiation. Soluble saccharide concentration in the corm reached a maximum after sprouting, probably due to rapid growth of the shoot. There was no change in the concentrations of soluble saccharides immediately prior to flower initiation, which suggested that quantitative changes in these saccharides were not responsible for activating flower initiation. When corms were treated with ethephon there was a greater degree of soluble saccharide mobilization during the first weeks of cultivation than in untreated corms. However, this did not seem to be the direct result of the addition of this phytohormone and was more probably the consequence of cell growth being encouraged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000332617753
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  • 6
    Electronic Resource
    Electronic Resource
    Bioproduction of neohesperidin and naringin in callus cultures of Citrus aurantium (1992)
    Springer
    Plant cell reports 11 (1992), S. 592-596 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The accumulation of both neohesperidin and naringin as major flavonoids in callus cultures of bitter orange (Citrus aurantium) was demonstrated using high performance liquid chromatography with a diode-array detector. The identity of both compounds was confirmed by their corresponding nuclear magnetic resonance spectra. The levels of neohesperidin are higher than those of naringin in callus culture, as they are in immature fruit, and high concentrations of both are found in young tissues such as immature fruits and the outer zone of calli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233099
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  • 7
    Electronic Resource
    Electronic Resource
    Accumulation of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus paradisi, Citrus limonia and Citrus aurantium (1991)
    Springer
    Plant cell reports 10 (1991), S. 410-413 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The production of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus species is described. The levels of these compounds were examined by gas chromatography-mass spectrometry and their yields were compared with the amounts found in mature fruits. A simultaneous increase and decrease in the levels of nootkatone and valencene, respectively, were observed with the aging of callus cultures of Citrus paradisi. These results suggest that valencene might be a possible precursor of nootkatone in this species. The high level of nootkatone detected in 9-month-old callus cultures of Citrus paradisi might be associated with the corresponding cell morphological changes observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232613
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  • 8
    Electronic Resource
    Electronic Resource
    Lateral diffusion of polarly transported indoleacetic acid and its role in the growth of Lupinus albus L. hypocotyls (1991)
    Springer
    Planta 185 (1991), S. 391-396 
    Details
    ISSN: 1432-2048
    Keywords: Auxin metabolism, transport ; Elongation growth ; Cortex-stele auxin distribution ; Lupinus (auxin transport) ; Polar auxin transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The transport and metabolism of indole-3-acetic acid (IAA) was studied in etiolated lupin (Lupinus albus L, cv. Multolupa) hypocotyls, following application of dual-isotope-labelled indole-3-acetic acid, [5-3H]IAA plus [1-14C]IAA, to decapitated plants. To study the radial distribution of the transported and metabolized IAA, experiments were carried out with plants in which the stele was separated from the cortex by a glass capillary. After local application of labelled IAA to the cortex, radioactivity remained immobilized in the cortex, near the application point, showing that polar transport cannot occur in the outer tissues. However, following application of IAA to the stele, radioactivity appeared in the cortex in those hypocotyl sections below the first 1 cm (in which the capillary was inserted), and the basipetal IAA movement was similar to that observed after application of IAA to the complete cut surface. In both assays, longitudinal distribution of 14C and 3H in the stele outside the first 1 cm was positively correlated with that of cortex, indicating that there was a lateral migration of IAA from the transport pathway (in the stele) to the outer tissues and that this migration depended on the amount of IAA in the stele. Both tissues (stele and cortex) exhibited intensive IAA metabolism, decarboxylation being higher in the stele than in the cortex while IAA conjugation was the opposite. Decapitation of the seedlings caused a drastic reduction of hypocotyl growth in the 24 h following decapitation, unless the hypocotyls were treated apically with IAA. Thus, exogenous IAA, polarly transported, was able to substitute the endogenous source of auxin (cotyledons plus meristem) to permit hypocotyl growth. It is proposed that IAA escapes from the transporting cells (in the stele) to the outer tissues in order to reach the growth-responsive cells. The IAA metabolism in the outer tissues could generate the IAA gradient necessary for the maintenance of its lateral flow, and consequently the auxin-induced cell elongation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201062
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  • 9
    Electronic Resource
    Electronic Resource
    Uptake and decarboxylation of indole-3-acetic acid during auxin-induced growth in lupin hypocotyl segments (1994)
    Springer
    Planta 193 (1994), S. 224-231 
    Details
    ISSN: 1432-2048
    Keywords: Acid-growth theory ; ATPase ; auxin (uptake, decarboxylation) ; Growth (auxin induced) ; Lupinus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elongation growth of etiolated hypocotyl segments of lupin (Lupinus albus L.) was stimulated by acid pH (4.6 versus 6.5) and by IAA for periods of up to 4 h. After this time, the segments were unable to grow further. In the presence of an optimal IAA concentration (10 μM), acid pH increased the growth rate but had no effect on final growth. With suboptimal IAA (0.1 μM), however, acid pH increased growth in a more than additive way, suggesting a synergistic action between the two factors. This synergism may be explained by the increased IAA uptake and decarboxylation seen at an acid pH. These results reinforce the view that the effects of low pH and IAA on growth are not independent. Vanadate inhibited growth and also IAA uptake and decarboxylation. This inhibitor, therefore, probably inhibits growth not only by decreasing ATPase-mediated acidification but also by decreasing H+-dependent IAA uptake from the apoplasm. This dependence of IAA uptake on ATPase may be mediated by apoplasmic acidification. The amount of IAA decarboxylated increased when the assay conditions favored the growth of segments, indicating that IAA could be destroyed by decarboxylation during the auxin-induced growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192534
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  • 10
    Electronic Resource
    Electronic Resource
    Identification of the metabolites of Indole-3-acetic acid in growing hypocotyls of Lupinus albus (1990)
    Springer
    Plant growth regulation 9 (1990), S. 315-327 
    Details
    ISSN: 1573-5087
    Keywords: IAA conjugation ; IAA decarboxylation ; HPLC ; Lupinus albus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The products of indole-3-acetic acid (IAA) metabolism by incubating hypocotyl sections and decapitated seedlings of Lupinus albus were investigated. Single treatments using [1-14C]-IAA, [2-14C]-IAA or [5-3H]-IAA and double treatments using [1-14C]-IAA+[5-3H]-IAA were carried out. Extracts from treated plant material were analyzed by paper chromatography (PC), Thin layer chromatography (TLC), and high performance liquid chromatography (HPLC). When hypocotyl sections were incubated in [2-14C]-IAA, several IAA decarboxylation products including indole-3-aldehyde (IA1), indole-3-methanol (IM), 3-hydroxymethyloxindole (HMOx), methyleneoxindole (MOx) and 3,3′-bisindolylmethane (BIM) were detected in the 95% ethanol extract; a latter extraction with 1M NaOH rendered IAA, IM and BIM, suggesting that conjugated auxins were formed in addition to conjugated IM. In sections incubated with [1-14C]-IAA, the 1M NaOH extraction also produced IAA so confirming the formation of conjugated auxins. The same decarboxylation products and two conjugated auxins, indole-3-acetylaspartic acid (IAAsp) and 1-O-(indole-3-acetyl)-β-D-glucose (IAGlu), were detected in the acetonitrile extracts from decapitated seedlings treated with [5-3H]-IAA. After a double isotope treatment ([1-14C]-IAA+[5-3H]-IAA) of decapitated seedlings, the ratio 14C/3H measured in the HPLC fractions of the acetonitrile extracts confirmed the presence of decarboxylation products as well as conjugated auxins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024917
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