Abstract
The elongation growth of etiolated hypocotyl segments of lupin (Lupinus albus L.) was stimulated by acid pH (4.6 versus 6.5) and by IAA for periods of up to 4 h. After this time, the segments were unable to grow further. In the presence of an optimal IAA concentration (10 μM), acid pH increased the growth rate but had no effect on final growth. With suboptimal IAA (0.1 μM), however, acid pH increased growth in a more than additive way, suggesting a synergistic action between the two factors. This synergism may be explained by the increased IAA uptake and decarboxylation seen at an acid pH. These results reinforce the view that the effects of low pH and IAA on growth are not independent. Vanadate inhibited growth and also IAA uptake and decarboxylation. This inhibitor, therefore, probably inhibits growth not only by decreasing ATPase-mediated acidification but also by decreasing H+-dependent IAA uptake from the apoplasm. This dependence of IAA uptake on ATPase may be mediated by apoplasmic acidification. The amount of IAA decarboxylated increased when the assay conditions favored the growth of segments, indicating that IAA could be destroyed by decarboxylation during the auxin-induced growth.
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Abbreviations
- AIG:
-
auxin (IAA)-induced growth
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We are grateful to Prof. R. Serrano (Dept. de Biotecnología, Universidad Politécnica, Valencia, Spain) for his critical comments and helpful suggestions, and to P. Thomas for proofreading the manuscript.
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Botía, J.M., Ortuno, A., Sabater, F. et al. Uptake and decarboxylation of indole-3-acetic acid during auxin-induced growth in lupin hypocotyl segments. Planta 193, 224–231 (1994). https://doi.org/10.1007/BF00192534
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DOI: https://doi.org/10.1007/BF00192534