Abstract
The production of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus species is described. The levels of these compounds were examined by gas chromatography-mass spectrometry and their yields were compared with the amounts found in mature fruits. A simultaneous increase and decrease in the levels of nootkatone and valencene, respectively, were observed with the aging of callus cultures of Citrus paradisi. These results suggest that valencene might be a possible precursor of nootkatone in this species. The high level of nootkatone detected in 9-month-old callus cultures of Citrus paradisi might be associated with the corresponding cell morphological changes observed.
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Abbreviations
- BAP:
-
benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- FID:
-
flame-ionisation detector
- FW:
-
fresh weight
- GLC:
-
gas liquid chromatography
- K:
-
Kinetin
- NAA:
-
naphthalene acetic acid
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Communicated by N. Amrhein
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del Río, J.A., Ortuño, A., Puig, D.G. et al. Accumulation of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus paradisi, Citrus limonia and Citrus aurantium . Plant Cell Reports 10, 410–413 (1991). https://doi.org/10.1007/BF00232613
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DOI: https://doi.org/10.1007/BF00232613