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  • 1
    ISSN: 1432-1432
    Keywords: Chloroplasts ; Evolution ; Rubisco ; Picoplankton ; Prochloron ; Prochlorothrix ; Prochlorococcus ; Prochlorales
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The prochlorophytes, oxygenic photosynthetic prokaryotes having no phycobiliprotein but possessing chlorophylls a and b, have been proposed to have a common ancestry with green chloroplasts, yet this is still controversal. We report here that partial sequence comparisons of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, including sequence data from two prochlorophytes, Prochlorococcus and Prochloron, indicate that Prochlorococcus is more closely related to a photosynthetic bacterium, Chromatium vinosum (γ-purple bacteria), than to cyanobacteria, while Prochloron is closely related to the prochlorophyte Prochlorothrix and to cyanobacteria. The molecular phylogenetic tree indicates that a common ancestor of Prochlorococcus and γ-purple bacteria branched off from the land plant lineage earlier than Prochloron, Prochlorothrix, and cyanobacteria.
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  • 2
    ISSN: 1432-072X
    Keywords: Prochloron ; Carbonic anhydrase ; Chloride inhibition ; Sullonamide inhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The prokaryotic algal symbiont of ascidians, Prochloron sp., was found to exhibit carbonic anhydrase activity which is largely associated with the cell surface. This extracellular carbonic anhydrase activity was inhibited, while the intracellular activity was not affected, by chloride or bromide. Acetazolamide and ethoxyzolamide inhibited carbonic anhydrase activity with I50 values of 7×10-4 and 3×10-4M, respectively. These I50 values are similar to those observed for intracellular carbonic anhydrases of Synechococcus sp. PCC7942, Chlamydomonas reinhardii and spinach.
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Aeropyrum pernix is the first strictly aerobic hyperthermophile known to grow heterotrophically at neutral pH and at temperatures up to 100°C. Using a simple and sensitive frit-fast atom bombardment liquid chromatography/mass spectrometry quinone analysis method, we analyzed the quinones in A. pernix. This organism contained demethylmenaquinone analogs (DMK-6(Hn)) and methionaquinone analogs (MTK-6(Hn)) when it was grown under vigorous shaking in the presence of air. The quinones were partially or fully saturated with six isoprenyl units. Although DMK and MTK are the quinones found in eubacteria, this is the first report to demonstrate the simultaneous occurrence of DMK and MTK in archaea. The effect of Na2S2O3 on the quinone composition was studied at concentrations of 0, 0.1 and 0.5% under aerobic growth conditions with shaking. The total quinone content was highest (83.4 μg g−1 dry cell weight) at 0.1% Na2S2O3. In the absence of Na2S2O3, only DMK-6 analogs were detected. While DMK analogs such as DMK-6(H12), DMK-6(H10) and DMK-6(H8) were the major quinones at 0.1% Na2S2O3, MTK analogs such as MTK-6(H12) and MTK-6(H10) were also detected. When the Na2S2O3 concentration was increased to 0.5%, both DMK-6(H8) and MTK-6(H10) disappeared, while MTK-6(H12) increased to approximately 20% of the total quinone content. When A. pernix was grown under oxygen limitation in a tightly closed bottle without gas phase, MK-6(H10) appeared.
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  • 4
    ISSN: 1440-1738
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Field surveys and trench excavation investigations revealed that there were at least four large seismic events produced by slips on the Gosukebashi fault in the Holocene in the southeastern Rokko Mountains of Japan. The characteristics of deformed topographies and three-dimensionally excavated exposures show that this fault is a right-lateral strike–slip fault having an average slip rate of 1.0 mm/year, with a reverse displacement component. The principle indicators of past faulting events are: (i) termination of secondary faults; (ii) sedimentary deposits related to faulting; and (iii) injection veins of fault gouge related to seismic faulting in the fractured zone. Radiocarbon dates indicate that the events occurred pre-1660 BC, 1660 BC–220 AD, from ∼ 30–220 to 600 AD and 15th century AD. The youngest event is probably associated with the large 1596 AD Keicho-Fushimi earthquake which occurred in the area around Kyoto and Kobe Cities. The second younger event is probably correlated with the 416 AD Yamato earthquake, which is the oldest historic earthquake in Japanese historic records. The results of trench surveys show that the horizontal displacement produced by an individual event is ∼ 1.5 m, and the recurrence of seismic event intervals is ∼ 1200 years in the Gosukebashi fault.
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  • 5
    ISSN: 1440-1738
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Abstract Cataclastic rocks found in the Disaster Prevention Research Institute, Kyoto University (DPRI) 500 m drill core and outcrops along the Nojima Fault zone on Awaji Island, southwest Japan, were examined at mesoscopic and microscopic scales. The damaged zone of this fault in granitic rocks, observed on the southeast side of the fault, is 50–60 m wide and is composed of fractured host rocks and cataclastic rocks including cataclasite, fault breccia, and fault gouge. The fault breccia and gouge of small scales are scattered in the damaged zone. Fault core (zone of extremely concentrated shearing deformation along a fault) consists of fault gouge measuring several tens to approximately 150 mm in width, as recognized both in the drill core and at outcrops of the Nojima Fault along which surface ruptures formed during the 1995 Kobe earthquake. Fault breccia, measuring a few meters wide, has developed pervasively in the damaged zone, just next to the fault core. Pseudotachylyte has been found interlayered with fault gouge within the fault core only at outcrops at Hirabarashi, not in the DPRI 500 m core. Petrological studies and powder X-ray diffraction analysis show that the pseudotachylyte and fault gouge are composed mainly of fine-grained angular clasts of the host granitic rocks, suggesting the pseudotachylyte is of ‘crush origin’. Foliated cataclasite is characterized by the preferred orientation of elongated biotite clasts and granular aggregates of quartz and feldspar clasts, and by the development of cataclastic shear bands. Unlike cataclastically deformed quartz and feldspar in the cataclasite, biotite in the foliated cataclasite shows combinations of brittle and plastic deformation, such as biotite ‘fish’, cleavage steps, bending and kinking. These textures suggest that the foliated cataclasite formed at a deeper level than the cataclasite, fault breccia and gouge, possibly before the Quaternary period during which the Nojima Fault has moved as a dextral strike–slip fault with some reverse movement resulting in the uplifting of Awaji Island. Examination of fault rocks from surface outcrops can yield similar results to those obtained from drill cores with regard to the internal structures of a fault zone.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 32 (1985), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In order to answer the question of how two dissimilar flagellar motions, retraction and undulation, of the longitudinal flagellum in Ceratium tripos are regulated, the effects of cationic milieu, calcium ionophore, calcium channel blockers and some anesthetics on the motion of the longitudinal flagellum were studied. The flagellum retracted and was installed in the sulcus in high K+, high Ca2+, low Na+-ASW (artificial sea water), and low Mg2+-ASW. Although Ca2+ ionophore X537A induced the retraction, it also induced disintegration of the flagellum. The Ca2+ channel blocker, La3+, prevented the retraction effectively in high K+-ASW and in low Mg2+-ASW but did not affect it in low Na−-ASW or in high Ca2+-ASW. Ruthenium red (RR), on the other hand, prevented the retraction in high Ca2+-ASW, low Na+-ASW, and low Mg2+-ASW but did not suppress the retraction induced in high K+-ASW. The organic Ca2+ antagonist, verapamil, or the local anesthetics, dibucaine and papaveline, did not prevent the retraction effectively in any ASW. These data suggest that the flagellum retracts when external Ca2+ enters into the flagellum. The dissimilar actions of La3+ and RR suggest that there may be two different sites for Ca2+ influx which have different affinity for La3+ or RR.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The longitudinal flagellum of Ceratium tripos moves in two dissimilar ways: undulation and retraction. The undulatory wave is planar and has a wavelength of 74.3 ± 9.6 μm and an amplitude of 14.2 ± 2.3 μm in sea water. The beat frequency is 30 Hz at 20°C, pH 8.0. The retractile motion is unique to Ceratium and is triggered by mechanical stimulation on the cell body, especially at the tip of the apical horn. When it retracts, the longitudinal flagellum folds every 4–5 μm along the flagellum. Cinematographic study showed that the flagellum folded from tip to base and was finally installed into the sulcus, a groove on the ventral side of the cell. This motion is completed in sea water within 28 msec. The retracted flagellum then re-extends and restores the undulation within a few seconds. The flagellum unfolds in the proximal portion first, then the distal, and finally the middle portion. Fixation always triggers the retraction. Scanning electron microscopy showed that the flagellum is folded and secondarily twisted in a helix. A new fiber in addition to the flagellar axoneme was found in the retracted flagellum by phase microscopy. This fiber (R-fiber) seems to contract during the retraction to fold the flagellum.
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  • 8
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and enolase are enzymes essential for glycolysis and gluconeogenesis. Dinoflagellates possess several types of both GAPDH and enolase genes. Here, we identify a novel cytosolic GAPDH–enolase fusion protein in several dinoflagellate species. Phylogenetic analyses revealed that the GAPDH moiety of this fusion is weakly related to a cytosolic GAPDH previously reported in dinoflagellates, ciliates, and an apicomplexan. The enolase moiety has phylogenetic affinity with sequences from ciliates and apicomplexans, as expected for dinoflagellate genes. Furthermore, the enolase moiety has two insertions in a highly conserved region of the gene that are shared with ciliate and apicomplexan homologues, as well as with land plants, stramenopiles, haptophytes, and a chlorarachniophyte. Another glycolytic gene fusion in eukaryotes is the mitochondrion-targeted triose-phosphate isomerase (TPI) and GAPDH fusion in stramenopiles (i.e. diatoms and oomycetes). However, unlike the mitochondrial TPI–GAPDH fusion, the GAPDH–enolase fusion protein appears to exist in the same compartment as stand-alone homologues of each protein, and the metabolic reactions they catalyze in glycolysis and gluconeogenesis are not directly sequential. It is possible that the fusion is post-translationally processed to give separate GAPDH and enolase products, or that the fusion protein may function as a single bifunctional polypeptide in glycolysis, gluconeogenesis, or perhaps more likely in some previously unrecognized metabolic capacity.
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  • 9
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The thermostability of the recombinant α- and β-subunit homo-oligomers (α16mer and β16mer) and of natural chaperonins purified from cultured Thermococcus strain KS-1 cells was measured to understand the mechanism for the thermal acclimatization of T. KS-1. The β-subunit content of the natural chaperonin from cells grown at 90°C was higher than that at 80°C. The optimum temperature for ATPase activity of the natural chaperonins was 80–90°C, whereas that for α16mer and β16mer was 60°C and over 90°C respectively. Judging from the ATPase activity, β16mer was more thermostable than α16mer. The thermostabilities of the natural chaperonins were intermediate between α16mer and β16mer, whereas the natural chaperonin with a higher β-subunit content was more stable than that with a lower β-subunit content. Native polyacrylamide gel electrophoresis (PAGE) revealed that the chaperonin oligomers thermally dissociated to their ATPase-inactive monomers. The thermal denaturation process monitored by circular dichroism showed that the free β-subunit was more stable than the free α-subunit, and that the secondary structure of the chaperonin monomer in the oligomer was more stable than that in the free monomer. These results suggest that the structure of these subunits was stabilized in the oligomer, and that an increase in the β-subunit content conferred higher thermostability to the natural hetero-oligomeric chaperonin.
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  • 10
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: To study the difference in expression of the chaperonin α- and β-subunits in Thermococcus strain KS-1 (T. KS-1), we measured their intracellular contents at various growth temperatures using subunit-specific antibodies. The β-subunit was significantly more abundant with increasing temperature (maximum at 93°C), whereas the α-subunit was not. Native PAGE with Western blot analysis indicated that the natural chaperonins in the crude extracts of T. KS-1 cells grown between 65°C and 95°C migrate as single bands with different mobility. The recombinant α- and β-subunit homo-oligomers migrated differently from each other and from natural chaperonins. Immunoprecipitation also showed that the natural chaperonin was the hetero-oligomer. These results indicate that chaperonin in T. KS-1 formed a hetero-oligomer with variable subunit composition, and that the β-subunit may be adapted to a higher temperature than the α-subunit. T. KS-1 probably changes its chaperonin subunit composition to acclimatize to the ambient temperature.
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