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  • 1
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    Polarization of chemoattractant receptor signaling during neutrophil chemotaxis (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2000-02-11
    Description: Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell's leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from Clostridium difficile, indicating that it requires activity of one or more Rho guanosine triphosphatases.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822871/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822871/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Servant, G -- Weiner, O D -- Herzmark, P -- Balla, T -- Sedat, J W -- Bourne, H R -- CA-54427/CA/NCI NIH HHS/ -- GM-25101/GM/NIGMS NIH HHS/ -- GM-27800/GM/NIGMS NIH HHS/ -- R01 GM027800-27/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Feb 11;287(5455):1037-40.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94143, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10669415" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/metabolism ; *Bacterial Proteins ; Bacterial Toxins/pharmacology ; Cell Membrane/enzymology ; *Cell Polarity ; Chemotactic Factors/pharmacology ; Chemotaxis, Leukocyte/*physiology ; Chromones/pharmacology ; Complement C5a/pharmacology ; Cytoplasm/enzymology ; Enzyme Inhibitors/pharmacology ; HL-60 Cells ; Humans ; Insulin/pharmacology ; Morpholines/pharmacology ; N-Formylmethionine Leucyl-Phenylalanine/pharmacology ; Neutrophils/enzymology/*physiology/ultrastructure ; Phosphatidylinositol 3-Kinases/antagonists & inhibitors/metabolism ; *Protein-Serine-Threonine Kinases ; Proto-Oncogene Proteins/*metabolism ; Proto-Oncogene Proteins c-akt ; Pseudopodia/enzymology ; Receptors, Formyl Peptide ; Receptors, Immunologic/*metabolism ; Receptors, Peptide/*metabolism ; Recombinant Fusion Proteins/metabolism ; *Signal Transduction ; rho GTP-Binding Proteins/antagonists & inhibitors/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Mitosis in living budding yeast: anaphase A but no metaphase plate (1997)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1997-07-25
    Description: Chromosome movements and spindle dynamics were visualized in living cells of the budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by expression of a protein fusion between green fluorescent protein (GFP) and the Lac repressor, which bound to an array of Lac operator binding sites integrated into the chromosome. Spindle microtubules were detected by expression of a protein fusion between GFP and Tub1, the major alpha tubulin. Spindle elongation and chromosome separation exhibited biphasic kinetics, and centromeres separated before telomeres. Budding yeast did not exhibit a conventional metaphase chromosome alignment but did show anaphase A, movement of the chromosomes to the poles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Straight, A F -- Marshall, W F -- Sedat, J W -- Murray, A W -- New York, N.Y. -- Science. 1997 Jul 25;277(5325):574-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, Box 0444, School of Medicine, University of California at San Francisco, San Francisco, CA 94143-0444, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9228009" target="_blank"〉PubMed〈/a〉
    Keywords: *Anaphase ; Bacterial Proteins/metabolism ; Centromere/chemistry/physiology ; Chromatids/physiology ; Chromosomes, Fungal/chemistry/*physiology ; *Escherichia coli Proteins ; Green Fluorescent Proteins ; Lac Repressors ; Luminescent Proteins ; *Metaphase ; Microscopy, Fluorescence ; Microtubules/ultrastructure ; *Mitosis ; Movement ; Operator Regions, Genetic ; Recombinant Fusion Proteins ; Repressor Proteins/metabolism ; Saccharomyces cerevisiae/*cytology ; Spindle Apparatus/physiology/ultrastructure ; Telomere/physiology ; Tubulin/analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    Block in anaphase chromosome separation caused by a telomerase template mutation (1997)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1997-03-07
    Description: Telomeres are essential for chromosome stability, but their functions at specific cell-cycle stages are unknown. Telomeres are now shown to have a role in chromosome separation during mitosis. In telomeric DNA mutants of Tetrahymena thermophila, created by expression of a telomerase RNA with an altered template sequence, division of the germline nucleus was severely delayed or blocked in anaphase. The mutant chromatids failed to separate completely at the midzone, becoming stretched to up to twice their normal length. These results suggest a physical block in mutant telomere separation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kirk, K E -- Harmon, B P -- Reichardt, I K -- Sedat, J W -- Blackburn, E H -- GM26259/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1997 Mar 7;275(5305):1478-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143-0414, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9045613" target="_blank"〉PubMed〈/a〉
    Keywords: *Anaphase ; Animals ; Base Sequence ; Chromatids/physiology ; Chromosomes/*physiology/ultrastructure ; DNA, Protozoan/genetics ; Micronucleus, Germline/ultrastructure ; Microscopy, Fluorescence ; Mitotic Index ; Mutation ; Phenotype ; RNA, Protozoan/genetics ; Repetitive Sequences, Nucleic Acid ; Telomerase/genetics/*metabolism ; Telomere/genetics/*physiology ; Templates, Genetic ; Tetrahymena thermophila/*cytology/genetics ; Transformation, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy (2008)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2008-06-07
    Description: Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916659/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916659/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Schermelleh, Lothar -- Carlton, Peter M -- Haase, Sebastian -- Shao, Lin -- Winoto, Lukman -- Kner, Peter -- Burke, Brian -- Cardoso, M Cristina -- Agard, David A -- Gustafsson, Mats G L -- Leonhardt, Heinrich -- Sedat, John W -- GM-2501-25/GM/NIGMS NIH HHS/ -- R01 GM025101/GM/NIGMS NIH HHS/ -- R01 GM025101-25/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2008 Jun 6;320(5881):1332-6. doi: 10.1126/science.1156947.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18535242" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Line ; Cell Nucleus/*ultrastructure ; Chromatin/*ultrastructure ; Fluorescent Dyes ; Heterochromatin/ultrastructure ; Imaging, Three-Dimensional/instrumentation/*methods ; Indoles ; Interphase ; Lamins/ultrastructure ; Mice ; Microscopy, Confocal ; Microscopy, Fluorescence/instrumentation/*methods ; Myoblasts ; Nuclear Envelope/*ultrastructure ; Nuclear Lamina/ultrastructure ; Nuclear Pore/ultrastructure ; Optics and Photonics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-09-24
    Description: n-Prop]yl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Giloh, H -- Sedat, J W -- GM25101/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1982 Sep 24;217(4566):1252-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7112126" target="_blank"〉PubMed〈/a〉
    Keywords: Fluorescein ; *Fluoresceins ; Free Radicals ; Gallic Acid/*analogs & derivatives ; Microscopy, Fluorescence/*methods ; Photochemistry ; *Propyl Gallate ; *Rhodamines ; *Xanthenes
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    The use of a charge-coupled device for quantitative optical microscopy of biological structures (1987)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1987-10-02
    Description: The properties of a charge-coupled device (CCD) and its application to the high-resolution analysis of biological structures by optical microscopy are described. The CCD, with its high resolution, high sensitivity, wide dynamic range, photometric accuracy, and geometric stability, can provide data of such high quality that quantitative analysis on two- and three-dimensional microscopic images is possible. For example, the three-dimensional imaging properties of an epifluorescence microscope have been quantitatively determined with the CCD. This description of the imaging properties of the microscope, and the high-quality image data provided by the CCD, allow sophisticated computational image processing methods to be used that greatly improve the effective resolution obtainable for biological structures. Image processing techniques revealed fine substructures in Drosophila embryonic diploid chromosomes in two and three dimensions. The same approach can be extended to structures as small as yeast chromosomes or to other problems in structural cell biology.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hiraoka, Y -- Sedat, J W -- Agard, D A -- GM25101-09/GM/NIGMS NIH HHS/ -- GM31627/GM/NIGMS NIH HHS/ -- GM32803-03/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1987 Oct 2;238(4823):36-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3116667" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Chromosomes/ultrastructure ; Drosophila melanogaster ; *Image Processing, Computer-Assisted ; Microscopy/*instrumentation ; Video Recording
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio (2013)
    National Academy of Sciences
    Details
    Publication Date: 2013-10-08
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 8
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    Computational adaptive optics for live three-dimensional biological imaging (2001)
    National Academy of Sciences
    Details
    Publication Date: 2001-03-27
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 9
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    Fast live simultaneous multiwavelength four-dimensional optical microscopy (2010)
    National Academy of Sciences
    Details
    Publication Date: 2010-08-12
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 10
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    Ultralow SNR 3D fluorescent image deconvolution [Cell Biology] (2013)
    National Academy of Sciences
    Details
    Publication Date: 2013-10-23
    Description: Four-dimensional fluorescence microscopy—which records 3D image information as a function of time—provides an unbiased way of tracking dynamic behavior of subcellular components in living samples and capturing key events in complex macromolecular processes. Unfortunately, the combination of phototoxicity and photobleaching can severely limit the density or duration of sampling, thereby...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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