Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate

Science. 1982 Sep 24;217(4566):1252-5. doi: 10.1126/science.7112126.

Abstract

n-Prop]yl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Fluorescein
  • Fluoresceins*
  • Free Radicals
  • Gallic Acid / analogs & derivatives*
  • Microscopy, Fluorescence / methods*
  • Photochemistry
  • Propyl Gallate*
  • Rhodamines*
  • Xanthenes*

Substances

  • Fluoresceins
  • Free Radicals
  • Rhodamines
  • Xanthenes
  • Gallic Acid
  • Propyl Gallate
  • Fluorescein