Publication Date:
1998-06-25
Description:
Long-term potentiation (LTP) at the Schaffer collateral-CA1 synapse involves interacting signaling components, including calcium (Ca2+)/calmodulin-dependent protein kinase II (CaMKII) and cyclic adenosine monophosphate (cAMP) pathways. Postsynaptic injection of thiophosphorylated inhibitor-1 protein, a specific inhibitor of protein phosphatase-1 (PP1), substituted for cAMP pathway activation in LTP. Stimulation that induced LTP triggered cAMP-dependent phosphorylation of endogenous inhibitor-1 and a decrease in PP1 activity. This stimulation also increased phosphorylation of CaMKII at Thr286 and Ca2+-independent CaMKII activity in a cAMP-dependent manner. The blockade of LTP by a CaMKII inhibitor was not overcome by thiophosphorylated inhibitor-1. Thus, the cAMP pathway uses PP1 to gate CaMKII signaling in LTP.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Blitzer, R D -- Connor, J H -- Brown, G P -- Wong, T -- Shenolikar, S -- Iyengar, R -- Landau, E M -- DK52054/DK/NIDDK NIH HHS/ -- GM54508/GM/NIGMS NIH HHS/ -- NS33646/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1998 Jun 19;280(5371):1940-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Bronx VA Medical Center and Department of Psychiatry, Mount Sinai School of Medicine, New York, NY 10029, USA. rb2@doc.mssm.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9632393" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Calcium-Calmodulin-Dependent Protein Kinase Type 2
;
Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
;
*Carrier Proteins
;
Cyclic AMP/analogs & derivatives/*metabolism/pharmacology
;
Cyclic AMP-Dependent Protein Kinases/metabolism
;
Electric Stimulation
;
Enzyme Inhibitors/metabolism/pharmacology
;
Hippocampus/*metabolism
;
In Vitro Techniques
;
*Intracellular Signaling Peptides and Proteins
;
*Long-Term Potentiation
;
Male
;
Phosphoprotein Phosphatases/antagonists & inhibitors/*metabolism
;
Phosphorylation
;
Protein Phosphatase 1
;
RNA-Binding Proteins/metabolism/pharmacology
;
Rats
;
Rats, Sprague-Dawley
;
Signal Transduction
;
Synapses/*metabolism
;
Thionucleotides/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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