Publication Date:
2005-05-28
Description:
MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid-modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wienholds, Erno -- Kloosterman, Wigard P -- Miska, Eric -- Alvarez-Saavedra, Ezequiel -- Berezikov, Eugene -- de Bruijn, Ewart -- Horvitz, H Robert -- Kauppinen, Sakari -- Plasterk, Ronald H A -- New York, N.Y. -- Science. 2005 Jul 8;309(5732):310-1. Epub 2005 May 26.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, the Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15919954" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blotting, Northern
;
Embryo, Nonmammalian/*metabolism
;
Embryonic Development
;
*Gene Expression
;
In Situ Hybridization
;
MicroRNAs/*genetics/*metabolism
;
Multigene Family
;
Oligonucleotide Array Sequence Analysis
;
Oligonucleotide Probes
;
Organ Specificity
;
Time Factors
;
Zebrafish/*embryology/*genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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