ISSN:
1432-2242
Keywords:
Key words Alfalfa
;
Repetitive DNA
;
FISH
;
Parentage identification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A highly repeated sequence (C300) was cloned from Medicago coerulea and its organization in the M. sativa-coerulea-falcata complex, M. arborea, and three somatic hybrids involving M. sativa, was investigated. Southern-blot analysis revealed a tandemly repeated array and a species-specificity of the sequence to those species belonging to the complex. Various degrees of amplification of C300 were detected among the species of the complex and the outcome in the somatic hybrids was dependent on parental composition. Sequence analysis revealed strong homology (96%) of C300 with a clone (E180) previously isolated from M. sativa. As FISH analysis showed that C300 was dispersed along the chromosomes of Medicago spp., it should prove a valid tool for establishing the chromosome origin of somatic hybrids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050619
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