ISSN:
1432-2048
Keywords:
Alcohol (long chain)
;
Ester synthetase
;
Hordeum (wax esters)
;
Wax ester biosynthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Experimental evidence for a membranebound microsomal ester synthetase from Bonus barley primary leaves is reported. The results are consistent with at least two mechanisms for the synthesis of barley wax esters: an acyl-CoA-fattyalcohol-transacylase-type reaction and an apparent direct esterification of alcohols with fatty acids. Biosynthesis of wax esters was not specific with regard to the chain length of the tested alcohols. The microsomal preparation readily catalyzed the esterification of C16-, C18-, C22- or C24-labelled alcohols with fatty acids of endogenous origin. Exogenous long-chain alcohols were exclusively incorporated into the alkyl moieties of the esters. Addition of ATP, CoA and-or free fatty acids was not effective in stimulating or depressing the esterifying activity of the microsomal fraction. Partial solubilization of the ester synthetase was obtained using phosphate-buffered saline.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00399913
Permalink