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  • 1
    Electronic Resource
    Electronic Resource
    Trends in the fertilization ofStreptomyces coelicolor A3(2) (1984)
    Springer
    Archives of microbiology 139 (1984), S. 83-86 
    Details
    ISSN: 1432-072X
    Keywords: Plasmids ; Fertility factors ; Recombination ; Chromosome transfer ; Melozygotes ; Streptomyces coelicolor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract SCP1 and SCP2 (in the SCP2* state) fertility plasmids ofStreptomyces coelicolor A3(2) elicit recombination in SCP1+×SCP1- or SCP2*×SCP2- crosses. The rate is essentially constant (c. 10-4) if referred to the plasmid-less parent, irrespective of extreme variations in the parent balance. In ‘interrupted matings’ the alleles of the plasmid-less parent gradually increase in frequency in successive samples. The mobilization of the chromosome of the plasmid-less strain appears to be the primary event in merozygote formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692717
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  • 2
    Electronic Resource
    Electronic Resource
    Mutagenesis of the cyanobacterium Spirulina platensis by UV and nitrosoguanidine treatment (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 83 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The production of Spirulina platensis cells resistant to 8-azaguanine or β-(2-thienyl)-dl-alanine following mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) and UV-irradiation is described. The conditions for the mutagenesis were determined by monitoring cell viability and the appearance of the two types of mutants as a function of the stage of growth of the tricomes and the length and the conditions of the treatment. The optimal conditions for UV and MNNG mutagenesis were found to be 1–3 min irradiation and 30 min incubation with 50 μg MNNG/ml of tricomes derived from cultures entering stationary phase sonicated for 10 s and 5 s respectively. Under these conditions β-(2-thienyl)-dl-alanine-resistant mutants appeared at a frequency ≥10−4 and ≥10−5 following UV- and MNNG-mutagenesis, respectively. Mutants resistant to 8-azaguanine were found at a frequency approx. 10−5 only after MNNG mutagenesis. A few chlorate-resistant mutants were also obtained following UV treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04394.x
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  • 3
    Electronic Resource
    Electronic Resource
    Production and regeneration of spheroplasts from the cyanobacterium Spirulina platensis (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 59 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The development of a micro-method for the production and regeneration of spheroplasts starting from S. platensis trichomes is presented. The influence of the growth stage along with different treatments and conditions on the efficiency of spheroplast formation and regeneration are analyzed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03098.x
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  • 4
    Electronic Resource
    Electronic Resource
    Chloramphenicol resistance in Streptomyces coelicolor A3(2): Possible involvement of a transposable element (1978)
    Springer
    Molecular genetics and genomics 164 (1978), S. 99-103 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The transfer of a Chl element, causing resistance to chloramphenicol in Streptomyces coelicolor A3(2), was studied in NF x SCP1− superfertile crosses. When the Chl element is on the donor side (NF) its transfer to the recombinant cells was virtually total as if the element acted as a second concomitant transfer origin. When the Chl element was on the recipient side (SCP1−) it was never displaced by the immigrant chromosome even when the region facing chl + was selected for. A fraction of the original Chl− mutants presented a requirement for arginine (ArgB−). A Chl− mutant gave rise spontaneously to ArgB− derivatives at high frequency. The same ArgB− requirement come out at high frequency among Chl− derivatives from a cross NFChl− x SCP1−Chl+ in which neither parent required arginine or produced spontaneously arginineless derivatives. It is suggested that the Chl element is a “transposable element” (Tn) presumably associated with “insertion sequences” (IS). The insertional inactivation of the Chl element may be accompanied or followed by a deletion in the adjacent ArgB gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267604
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  • 5
    Electronic Resource
    Electronic Resource
    Evidence of heterokaryosis in Streptomyces coelicolor A3(2) (1984)
    Springer
    Molecular genetics and genomics 194 (1984), S. 541-544 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hyphae from mixed cultures of complementary auxotrophs of Streptomyces coelicolor A3(2) did not grow on minimal media (MM) when fertility plasmids (SCP1 and SCP2) were missing in both strains. The addition of one part per cent of complete medium (CM) to MM allowed growth of vigorous colonies among the tiny colonies of the parental types. The former, amounting to 1%–10% of the total population, turned out to be heterokaryons. They could be propagated on the same medium by plating of hyphal fragments. When five parts per cent of CM were added to MM, beside the heterokaryotic colonies vigorous ‘spindles’ of aerial mycelium were formed whenever complementary colonies overlapped. When the SCP1 and SCP2 plasmids were present in one or both parents a clear constraint on the outburst of heterokaryotic aerial mycelium was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425573
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  • 6
    Electronic Resource
    Electronic Resource
    Properties of transposon SCTn1 of Streptomyces coelicolor A3(2) (1983)
    Springer
    Molecular genetics and genomics 191 (1983), S. 158-161 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloramphenicol resistance (Cmlr) of Streptomyces coelicolor A3(2) behaves like a transposon locus, not being localisable in any region of the map and yet being transferable in crosses at a rate comparable to that of chromosomal markers. It can, also be transposed onto a plasmid (SCP1) and back to the chromosome. Some traits, such as arginino-succinate synthase production (ArgG), aerial mycelium formation (AmyA), resistance to tetracycline and to rifamycin C appear to be joined to Cml in three processes: co-mutation, i.e. simultaneous loss, post-mutation, i.e. spontaneous loss at high, frequency in subclones from Cmls strains, co-transfer, i.e. joint transfer with the cml locus in crosses or during infection by the aggregate SCP1::SCTn1 plasmid. All these processes have been consistently observed with special attention to the argG locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330904
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  • 7
    Electronic Resource
    Electronic Resource
    Cloning and characterization of the gene encoding an esterase from Spirulina platensis (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 124-126 
    Details
    ISSN: 1617-4623
    Keywords: Microalgae ; Cyanobacteria ; Esterase ; Gene cloning ; DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene encoding a 23 kDA serine esterase from the cyanobacterium Spirulina platensis has been identified, cloned, characterized and expressed in Escherichia coli. The primary structure of the esterase deduced from the DNA sequence displayed 32% sequence identity with the carboxylesterase (esterase II) encoded by estB of Pseudomonas fluorescens; the highest degree of homology is found in a stretch of 11 identical or highly conserved amino acid residues corresponding to the GXSXG consensus motif found in the catalytic site of many serine proteases, lipases and esterases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283885
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  • 8
    Electronic Resource
    Electronic Resource
    A jumping gene in Streptomyces coelicolor A3(2) (1980)
    Springer
    Molecular genetics and genomics 177 (1980), S. 453-458 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The difficulty in mapping the gene for chloramphenicol resistance (cml R) in Streptomyces coelicolor A3(2) stock strains is possibly due to its location on different sites of the chromosome in various mixed subelones. Fresh isolates from CmlR strains show single unequivocal locations of cml R. The same holds for CmlR strains derived as revertants from CmlS variants. The two best established sites for cml R are one between cys A and met A, the other at right of arg A, possibly in the right empty arc of the map (Fig. 2). The cml R gene was assumed to be on a transposon (SCTn1), together with a gene for arginine-succinate synthase (argG), a gene for chromosome transfer (tra) and a gene for aereal mycelium formation (amy). In a CmlR revertant, the cml R gene appears disjoined from argG (Fig. 5), thus showing the ability of SCTnl to be split and partially transposed. The possible wide occurrence of transposons in the genus Streptomyces is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271484
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  • 9
    Electronic Resource
    Electronic Resource
    The electrophoretic karyotype of two strains of Candida albicans by transverse alternate field electrophoresis reveals higher number of chromosomes ranging from 1 to 3·5 Mb (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 9 (1993), S. 1213-1218 
    Details
    ISSN: 0749-503X
    Keywords: Candida albicans ; chromosome size ; karotype ; pulsed field gel electrophoresis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The advent of the powerful electrophoretic technique, pulsed field gel electrophoresis, first developed on the yeast Saccharomyces cerevisiæ, has brought a vital impulse to the genetic study on the opportunistic pathogen Candida albicans. We report here on sizing and numbering of Candida chromosomes using transverse alternate field electrophoresis. Our results indicate the occurrence of nine to ten electrophoretic bands (depending on type of Candida strain), that range in approximate size from 1 to 3·5 Mbp, and may account for a higher overall chromosome number, because at least two of these bands appear to be doublets. This number of bands, with smaller size, is considerably higher than previously reported.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320091108
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  • 10
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    Trends in the fertilization ofStreptomyces coelicolor A3(2) (1984)
    Springer
    Details
    Publication Date: 1984-09-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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