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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Applied Soil Ecology 1 (1994), S. 291-298 
    ISSN: 0929-1393
    Keywords: Earthworms ; Food location ; Microscolex ; Population density
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 18 (1994), S. 241-244 
    ISSN: 1432-0789
    Keywords: Earthworms ; Food location ; Population density ; Aporrectodea ; Microscolex ; Organic manure Cocoon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was established to assess the effects of additional organic matter on earthworm populations. Bags of soil (15 cm deep) were placed in a pasture at Balhannah, South Australia, at the beginning of autumn 1991. The bags were initially seeded with five individuals ofAporrectodea trapezoides, and 250 g dried sheep manure was added to most bags. Manure was added either in pellet or milled form, and applied either on the surface, in the 5–10 cm layer, or evenly dispersed over 15 cm. In harvests during weeks 7,9, and 11 after the start of the experiment,A. trapezoides and three other species,A. caliginosa, A. rosea, andMicroscolex dubius, were recovered from the bags. Bags with added manure had significantly higher numbers of each species than bags with no manure. During the 4-week sampling period (weeks 7–11) the numbers ofM. dubius recovered per bag decreased, whileA. rosea increased. Total earthworm numbers were not influenced by either the form or the location of application of the manure. Earthworms were sampled from three depths, 0–5, 5–10, and 10–15 cm. Both numbers and biomass of earthworms were positively correlated with the location of the manure. Cocoons ofAporrectodea spp. were more abundant when the manure was milled and evenly dispersed, and were consistently located in the lower soil layers, regardless of the form or location of manure.
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  • 3
    ISSN: 1432-0789
    Keywords: Key words Earthworms ; Burrowing behaviour ; Food location ; Aporrectodea Microscolex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A series of experiments was conducted over 96 h in 240-mm-deep soil microcosms, to assess the effect of the presence and distribution of sheep manure over the soil surface on the vertical and horizontal distribution of burrows and numbers of the earthworms Aporrectodea trapezoides and Microscolex dubius. Within some microcosms the dung was placed on half of the soil surface and this caused aggregation, with over two-thirds of the earthworms being found in the soil directly under the manure. The presence of surface-applied sheep manure caused both species to aggregate in the surface soil. In contrast, without manure, A. trapezoides was evenly distributed throughout the soil profile while M. dubius aggregated in the deeper soil. The pattern of burrow construction was also influenced by the presence of surface manure. In the absence of manure, burrows of both species were evenly distributed through the soil, but in the presence of surface manure M. dubius constructed proportionally more burrows close to the surface. Both species constructed approximately twice the burrow area in the absence than in the presence of surface manure. For both species the daily rate of burrow construction decreased over the experimental period. From these data we inferred that there was more widespread and active foraging behaviour in both species when organic food material was scarce. M. dubius differed from A. trapezoides in that it more strongly concentrated foraging activity in the vicinity of the manure food source.
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  • 4
    ISSN: 1432-0789
    Keywords: Earthworms ; Burrowing behaviour ; Food location ; Aporrectodea Microscolex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A series of experiments was conducted over 96 h in 240-mm-deep soil microcosms, to assess the effect of the presence and distribution of sheep manure over the soil surface on the vertical and horizontal distribution of burrows and numbers of the earthworms Aporrectodea trapezoides and Microscolex dubius. Within some microcosms the dung was placed on half of the soil surface and this caused aggregation, with over two-thirds of the earthworms being found in the soil directly under the manure. The presence of surface-applied sheep manure caused both species to aggregate in the surface soil. In contrast, without manure, A. trapezoides was evenly distributed throughout the soil profile while M. dubius aggregated in the deeper soil. The pattern of burrow construction was also influenced by the presence of surface manure. In the absence of manure, burrows of both species were evenly distributed through the soil, but in the presence of surface manure M. dubius constructed proportionally more burrows close to the surface. Both species constructed approximately twice the burrow area in the absence than in the presence of surface manure. For both species the daily rate of burrow construction decreased over the experimental period. From these data we inferred that there was more widespread and active foraging behaviour in both species when organic food material was scarce. M. dubius differed from A. trapezoides in that it more strongly concentrated foraging activity in the vicinity of the manure food source.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 49 (1996), S. 412-420 
    ISSN: 0006-3592
    Keywords: Amycolatopsis orientalis ; vancomycin production ; chemostat culture ; phosphate inhibition ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Production of the glycopeptide antibiotic vancomycin by two Amycolatopsis orientalis strains was examined in batch shake flask culture in a semidefined medium with peptone as the nitrogen source. Different growth and production profiles were observed with the two strains; specific production (Yp/x) was threefold higher with strain ATCC 19795 than with strain NCIMB 12945. A defined medium with amino acids as the nitrogen source was developed by use of the Plackett-Burman statistical screening method. This technique identified certain amino acids (glycine, phenylalanine, tyrosine, and arginine) that gave significant increased specific production, whereas phosphate was identified as inhibitory for high specific vancomycin production. Experiments made with the improved medium and strain ATCC 19795 showed that vancomycin production kinetics were either growth dissociated or growth associated, depending on the amino acid concentration. In chemostat culture at a constant dilution rate (0.087 h-1), specific vancomycin production rate (qvancomycin) decreased linearly as the medium phosphate concentration was increased from 2 to 8 mM. In both phosphate and glucose limited chemostats, qvancomycin was a function of specific growth rate; the maximum value was observed at D = 0.087 h-1 (52% of the maximum specific growth rate). Under phosphate limited growth conditions, qvancomycin was threefold higher (0.37 mg/g dry weight/h) than under glucose limitation (0.12 mg/g dry weight/h). © 1996 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 12 (1970), S. 273-290 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The concept of a “critical oxygen concentration” is conventionally considered to hold for the submerged aerobic fermentation of glucose to gluconic acid. Above the critical level the fermentation rate is supposedly independent of oxygen concentration. In this work it is shown that, at a given agitation rate, the fermentation is independent of dissolved oxygen when above the critical. However, an increase in the agitation rate results in an increase in the fermentation rate. This increase was shown to be accompanied by an increase in the gluconolactone concentration in the broth. Gluconolactone, an intermediate in the reaction pathway, is hydrolyzed nonenzymatically to gluconic acid. Evidence is presented to suggest that the increased gas-liquid interfacial area brought about by increased agitation causes an increased net rate of lactone formation. This in turn results in an increased rate of hydrolysis of the lactone to gluconic acid. A model is presented hypothesizing that negatively charged cells adsorb at the gas-liquid interface. These cells attract hydrogen ions, causing a lowering of the pH in the film around the bubbles. It is this lowered pH which is considered to bring about increased fermentation rates when the interfacial area is increased. Supporting evidence is presented.
    Additional Material: 4 Ill.
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  • 7
    ISSN: 0006-3592
    Keywords: chemostat ; glucose limitation ; glycosylation ; CHO cells ; interferon-γ ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The physiology of a recombinant Chinese hamster ovary cell line in glucose-limited chemostat culture was studied over a range of dilution rates (D = 0.008 to 0.20 h-1). The specific growth rate (μ) deviated from D at low dilution rates due to an increased specific death rate. Extrapolation of these data suggested a minimum specific growth rate of 0.011 h-1 (μmax = 0.025 h-1) The metabolism at each steady state was characterized by determining the metabolic quotients for glucose, lactate, ammonia, amino acids, and interferon-γ (IFN-γ). The specific rate of glucose uptake increased linearly with μ, and the saturation constant for glucose (Ks) was calculated to be 59.6 μM. There was a linear increase in the rate of lactate production with a higher yield of lactate from glucose at high growth rates. The decline in the rate of production of lactate, alanine, and serine at low growth rate was consistent with the limitation of the glycolytic pathway by glucose. The specific rate of IFN-γ production increased with μ in a manner indicative of a growth-related product. Despite changes in the IFN-γ production rate and cell physiology, the pattern of IFN-γ glycosylation was similar at all except the lowest growth rates where there was increased production of nonglycosylated IFN-γ. © 1993 John Wiley & Sons, Inc.
    Additional Material: 10 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 785-804 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The physiology of Aspergillus nidulans strain 224 has been studied under conditions of batch- and glucose-limited chemostat-culture and the effect of different steady state growth rates and dissolved oxygen tensions (DOT) examined. Measurements of the specific activities of selected glucose enzymes, the extent of oxygen uptake inhibition by glycolytic inhibitors, and radiorespirometric analyses were made in order to follow the variations in glucose catabolism, which occurred under these conditions. Greatly increased activity of the hexosemonophosphate (HMP) pathway was found during: (i) exponential growth of batch cultures; (ii) at near maximum specific growth rates (μ = 0.072 hr-1) (DOT = 156 mm Hg); and (iii) at low DOT levels (〈30 mm Hg) (μ = 0.050 hr-1) in chemostat cultures. These changes in glucose eatabolism have been discussed in terms of the biosynthetic demands of the fungus under the influence of changing growth pressures. Preliminary studies also have been made of transition state behavior following stepwise alteration of the DOT. A new steady state was established after 4-5 culture doublings during which period an “overshoot” in HMP pathway activity occurred; these kinetics are indicative of a derepression of certain glucose enzymes. Low molecular weight phenols are synthesized during the exponential phase in batch cultures and these are further metabliized to a major secondary metabolite, melanin, at the onset of stationary phase conditions. The kinetics of tyrosinase production in steady state chemostats differs from those that might be predicted for an enzyme associated solely with secondary metabolism. A primary physiological role for this oxidase in Aspergillus nidulans has been postulated.
    Additional Material: 11 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 1054-1065 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Four fluidized bed reactors were used to evaluate single-and separated-phase anaerobic treatments of a high strength wastewater. Two reactors were fed with a synthetic wastewater, containing glucose as the primary carbon source, with a COD of 1.2 × 104 mg/L while the remaining pair were fed with a wastewater with a COD of 6000 mg/L. AT each influent strength, one fluidized bed reactor was operated as a single-phase system while the other was operated as a methanogenic reactor which was preceded by an acidification reactor in a separatedphase system. The reactors were operated under steady-state and variable process conditions. The separated-phase system consistently gave a better quality effluent with lower effluent suspended solids and total COD, and the methane yield was also improved. Under variable process conditions, the separated-phase system was inherently more stable and recovered more rapidly following a shock loading. Propionate and acetate degradation studies indicated that the biomass in the methanogenic fluidized beds of the two-phase systems was more adapted to volatile acid degradation than the biomass in the single-phase fluidized beds.
    Additional Material: 9 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 827-827 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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